Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0014721

Physical Location & Seq

Genomic Coordinates Forward (+)

54065538 .. 54068960

Gene Structure 3423 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G027540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2475 bp

ATGTTGGGTGAGGAAGAAGAGTATGGCCCTGCCGCCGGAAATACTACCCGGAAAAGTTATCTCCGGTCCATGTCATGGAGTGACCGTTCACCTTCCAAACCTAATCCTTATCCAAAGCCTCAGCTCAATAGTAAAGCAAGGTCTTGCTTGCCTCCACTTCAGCCTCTTTCAATAGCCAAGAGTAATGTTAAGGAGTGGCCAAAGGCGGGTTCTGATGATCTTGGTGTCTGGCCTCAGCCCCCAACCCCTAGAGGGTCGGTTAAACCCTTTCCGGCTTGCTTTTTTGATAAAGAGTGCTCCAGAATTGCAGAGCATATATACTTGGGGAGTGATGCTGTGGCTAAGAACCGTGAGGTTTTGAGGAAGAATGGGATCACCCATGTGCTCAACTGCGTTGGGTTTGTGTCTCCTGAGTATTTCAAGAATGATCTTGTGTACAAGACGCTCTGGTTGAAAGATAGCCCCTCGGAGGACATTACGAGCATTCTGTATGATGTGTTTGATTATTTTGAAGATGTGAGGAAGCAAAGTGGGAGAGTTCTTGTGCATTGTTGTCAGGGAGTGTCGCGGTCAACGGCTCTCGTGATTGCATACCTTATGTGGAGGGAGGGCCAGAGCTTTGAAGATGCATTTCAGTATGTCAAGGCAGCAAGAGGGGTGACAAACCCAAATATGGGTTTTGCCTGTCAACTTCTGCAGTGCCAGAAGCGGGTGCATGCTGTGCCTGCAAGCCCCAGTTCTGTGTTAAGGATGTACAGGATGGCCCCGCATTCATCGTATGATCCTCTTCATCTGGTGCCAAAGATGTTAAACCATCCAGGTCCACAAGGACTTGACTCTCGTGGTGCGTTTGTTGTGCATGTTCCATCTGCTATATATGTCTGGATTGGAAAGGATTGCAACACAATGATGTCAGACAACGCAAGAGCAGCTGCCCTTCAGGTCATCCAGTATGAGAGGGCAAAGGGTCCAATTATGGACATCAATGAAGGCAAGGAGCCACCAGAATTTTGGGACGCTCTTTCCAGTGGTCTTTCAGCAGAGGATTGCGGCAAGGGAGATGTCCAAAAGGAAGAAACTCTTTCTTCTGGAGGCGATAAGGTTGCTGCAGTGACTTGTATTCCGGTGGGTGAAAGAAAGGTTGGTGAATATGATTTGGATTTCGAAATTTTCAGTAAGGCACTTGCAGGTGGGGTTGTTCCACCATTTTCTGTATCAAATACTGAGTCGGAAACTTGCCTTCCGGCCAGAGAAAATGGATGGGGCAGATTGCGGCAAAAGTTTGCTAGTGGAATTATGAAGGATTTGGTCACATCTTCTGAGCTGAACTGTAAAACTACCCCATCCAGTGATGGATTGGATATGGTTGTGGAAGTTAACAAAGAAGCAGAAGACCCTGTTTCCTTGATTGAGCCTTTATCACCATTGTCAGCGCCAAGCCACTTTTGTGGTTCACCAGATTCCTTTGAGTGTTATCCAATTAGTAGCCCAAGTAGGATAAGAGATACATGTCTGGAAGTAGGACACTCTGTTGCTCTTACCAATCCATTATCGTTTCCGACAACTGACTGTGGGTCACCCAATTCTTGTTCTTGGTTTCCTGATAACAATCTTAAGTTTAGCTCAAAATCCCCAACACTCTCCCCTTCAACCTCTGAATACTCTTGTTCATCATTTTCCTTTTCACCCTCATCTTCTAATTGGTCGGACTTGTCATACTTGTCTTCTCAACAGCCTTCACCTTCAGGCTTGGATTCTGCAGATCCATTATGTATTAAGCATAGTTCCATAGCCGATAGCTCACAGTTACCTTTTAAAGAATCTCTTTCTTCACCTAGAGAGGCATTTTCAGCTAATCGTAGTTTGGAAGTGACAAATACATGTTCGACATGTAAAGGGATTTCCCCTTCTATTGCAGAGCGTAGAGGGAGTAAACCTCCACCTCTAATGTTGCTACCCTCAGTTGAAGAATCACCTAAAGTTTCGAGGAATTTGGTACGCTCATGGTCGTTTTCCTTACCTGACTTGGATGATGATCTGATGGACACTGATTGCAACCAAGCCGAAAATGTGAGCAACAGAGAAGAGCTAATGTTGGATGTTGACATTGGTAACCCTGAAAATGAATTACAATCTGAAGCGACCAGGGAAGCTGCAGAGGTGATTAACCCTGTTTTGTACCAGTGGCCTTCTTTGAACAAAGTGGTGATGCATCAGTCTGCCAAACTTCATTCTACATCAGCATATCTTTTGCTAGCTCCAGATGCGACTGTGGGTGCAAATAAACCTGGTGTCTTATTTAAAGGACAGAATGCAACATTTGAGGATAGCCATCTTCACTGGGAGACCATAGGACGCTGTTTCCTTGAGCATATGGGTTTGCCTATGAGTGCCACTGTAAAGATAGTAAGAGAAGGTGAGGAGCCAGAGCAGTTCCTGATCCATCTCAGCTGTAAATCAATACAGAACACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000188 GO:0001704 GO:0001706 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007369 GO:0007492 GO:0008150 GO:0008152 GO:0008330 GO:0009653 GO:0009790 GO:0009888 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033549 GO:0033673 GO:0035335 GO:0035970 GO:0036211 GO:0042325 GO:0042326 GO:0042578 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043407 GO:0043408 GO:0043409 GO:0043412 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045936 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048598 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0070372 GO:0070373 GO:0071704 GO:0071900 GO:0071901 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902532 GO:1990439

KEGG Pathways

Metabolic & Signaling

ko04010 ko04726 ko05418 map04010 map04726 map05418

Pfam Domains

Protein Families

DSPc Gelsolin

Protein Analysis

824

Amino Acids

90.39

Weight (kDa)

5.35

Isoelectric Point (pI)

56.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	106 - 236	3.3e-28	Dual specificity phosphatase, catalytic domain
MPK1_gelsolin_C	PF25466	728 - 816	1.1e-17	MPK1 gelsolin domain C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014721)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25430 FvH4_4g25430
malus_domestica	MD13G1103000.v1.1 MD16G1103200.v1.1
prunus_persica	Prupe.1G242800_v2.0.a1
pyrus_communis	pycom16g08800
rosa_chinensis	RchiOBHm_Chr4g0432741
rosa_laevigata	RLG00000006816
rosa_multiflora	Rmu_co8494851.1_g000001 Rmu_sc0012180.1_g000009
rosa_roxburghii	Rroxscaffold_5G00374200
rosa_rugosa	Rorug04G0262100
rosa_samantha	Rh4AG316600 Rh4BG324700 Rh4CG339700 Rh4DG319900
rosa_wichuraiana	Rw4G027540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1178
Acc36I	ACCTGC	1 cut(s)	1178
AccB1I	GGYRCC	1 cut(s)	796
AccB7I	CCANNNNNTGG	1 cut(s)	75
AccII	CGCG	1 cut(s)	568
AciI	CCGC	7 cut(s)	33, 206, 568, 709, 767, 1050, 1273
AclWI	GGATC	4 cut(s)	380, 776, 1757, 2434
AcoI	YGGCCR	2 cut(s)	197, 1245
AcsI	RAATTY	3 cut(s)	1007, 1167, 1990
AcuI	CTGAAG	4 cut(s)	143, 923, 1728, 2157
AfaI	GTAC	4 cut(s)	437, 755, 1998, 2180
AfiI	CCNNNNNNNGG	8 cut(s)	35, 75, 206, 252, 469, 673, 674, 709
AflII	CTTAAG	1 cut(s)	1613
AflIII	ACRYGT	3 cut(s)	1508, 1880, 1889
AgsI	TTSAA	8 cut(s)	171, 421, 454, 512, 623, 1650, 1967, 2197
AhdI	GACNNNNNGTC	1 cut(s)	1573
AjnI	CCWGG	3 cut(s)	817, 2144, 2287
AjuI	GAANNNNNNNTTGG	4 cut(s)	193, 225, 1125, 1157
AleI	CACNNNNGTG	2 cut(s)	380, 1446
AloI	GAACNNNNNNTCC	2 cut(s)	70, 102
Alw21I	GWGCWC	2 cut(s)	299, 387
Alw26I	GTCTC	2 cut(s)	411, 2339
AlwI	GGATC	4 cut(s)	380, 776, 1757, 2434
AlwNI	CAGNNNCTG	1 cut(s)	2437
AoxI	GGCC	7 cut(s)	25, 197, 230, 610, 762, 1245, 2186
ApeKI	GCWGC	5 cut(s)	647, 929, 932, 1106, 2153
ApoI	RAATTY	3 cut(s)	1007, 1167, 1990
ArsI	GACNNNNNNTTYG	2 cut(s)	1211, 1243
AspLEI	GCGC	1 cut(s)	1435
AspS9I	GGNCC	6 cut(s)	26, 66, 610, 763, 821, 968
AsuC2I	CCSGG	1 cut(s)	49
AsuII	TTCGAA	1 cut(s)	1164
AsuNHI	GCTAGC	1 cut(s)	2254
AvaII	GGWCC	3 cut(s)	66, 821, 968
BalI	TGGCCA	1 cut(s)	199
BanI	GGYRCC	1 cut(s)	796
BauI	CACGAG	2 cut(s)	581, 840
BbsI	GAAGAC	2 cut(s)	1398, 1716
Bbv12I	GWGCWC	2 cut(s)	299, 387
BbvCI	CCTCAGC	2 cut(s)	120, 234
BbvI	GCAGC	5 cut(s)	659, 919, 941, 1093, 2140
BccI	CCATC	9 cut(s)	754, 822, 874, 1254, 1346, 1351, 2035, 2340, 2451
BceAI	ACGGC	1 cut(s)	591
BcgI	CGANNNNNNTGC	2 cut(s)	1085, 1119
BciT130I	CCWGG	3 cut(s)	819, 2146, 2289
BcnI	CCSGG	1 cut(s)	49
BcoDI	GTCTC	2 cut(s)	411, 2339
BfaI	CTAG	4 cut(s)	249, 1287, 1836, 2255
BfmI	CTRYAG	4 cut(s)	695, 1107, 1758, 2154
BfoI	RGCGCY	1 cut(s)	1436
BfrI	CTTAAG	1 cut(s)	1613
BfuAI	ACCTGC	1 cut(s)	1178
BisI	GCNGC	8 cut(s)	33, 648, 930, 933, 1051, 1107, 1274, 2154
BlsI	GCNGC	8 cut(s)	34, 649, 931, 934, 1052, 1108, 1275, 2155
Bme1390I	CCNGG	4 cut(s)	49, 819, 2146, 2289
Bme18I	GGWCC	3 cut(s)	66, 821, 968
BmeRI	GACNNNNNGTC	1 cut(s)	1573
BmgT120I	GGNCC	6 cut(s)	26, 66, 610, 763, 821, 968
BmiI	GGNNCC	5 cut(s)	765, 798, 969, 999, 2424
BmrFI	CCNGG	4 cut(s)	49, 819, 2146, 2289
BmrI	ACTGGG	2 cut(s)	729, 2350
BmsI	GCATC	5 cut(s)	322, 616, 2199, 2221, 2254
BmtI	GCTAGC	1 cut(s)	2258
BmuI	ACTGGG	2 cut(s)	729, 2350
BpiI	GAAGAC	2 cut(s)	1398, 1716
BplI	GAGNNNNNCTC	2 cut(s)	1921, 1953
BpmI	CTGGAG	3 cut(s)	283, 1110, 2244
Bpu10I	CCTNAGC	2 cut(s)	120, 234
Bpu14I	TTCGAA	1 cut(s)	1164
BpuEI	CTTGAG	1 cut(s)	2387
BpuMI	CCSGG	1 cut(s)	49
BsaBI	GATNNNNATC	3 cut(s)	1608, 2034, 2331
BsaI	GGTCTC	1 cut(s)	2339
BsaJI	CCNNGG	2 cut(s)	465, 2145
BsaWI	WCCGGW	2 cut(s)	63, 1123
BsaXI	ACNNNNNCTCC	2 cut(s)	70, 100
Bsc4I	CCNNNNNNNGG	8 cut(s)	35, 75, 206, 252, 469, 673, 674, 709
Bse1I	ACTGG	6 cut(s)	735, 949, 1026, 1347, 2182, 2345
Bse8I	GATNNNNATC	3 cut(s)	1608, 2034, 2331
BseBI	CCWGG	3 cut(s)	819, 2146, 2289
BseDI	CCNNGG	2 cut(s)	465, 2145
BseGI	GGATG	8 cut(s)	756, 765, 814, 945, 1265, 1343, 2035, 2104
BseJI	GATNNNNATC	3 cut(s)	1608, 2034, 2331
BseLI	CCNNNNNNNGG	8 cut(s)	35, 75, 206, 252, 469, 673, 674, 709
BseMII	CTCAG	7 cut(s)	134, 248, 402, 1215, 1311, 1974, 2461
BseNI	ACTGG	6 cut(s)	735, 949, 1026, 1347, 2182, 2345
BseRI	GAGGAG	1 cut(s)	2435
BseXI	GCAGC	5 cut(s)	659, 919, 941, 1093, 2140
Bsh1236I	CGCG	1 cut(s)	568
BshFI	GGCC	7 cut(s)	27, 199, 232, 612, 764, 1247, 2188
BshNI	GGYRCC	1 cut(s)	796
BsiHKAI	GWGCWC	2 cut(s)	299, 387
BsiSI	CCGG	6 cut(s)	36, 49, 64, 272, 1124, 1244
BslFI	GGGAC	1 cut(s)	1028
BslI	CCNNNNNNNGG	8 cut(s)	35, 75, 206, 252, 469, 673, 674, 709
BsmAI	GTCTC	2 cut(s)	411, 2339
BsmFI	GGGAC	1 cut(s)	1028
BsmI	GAATGC	3 cut(s)	483, 769, 2317
BsnI	GGCC	7 cut(s)	27, 199, 232, 612, 764, 1247, 2188
Bso31I	GGTCTC	1 cut(s)	2339
Bsp119I	TTCGAA	1 cut(s)	1164
Bsp1286I	GDGCHC	2 cut(s)	299, 387
Bsp1407I	TGTACA	2 cut(s)	435, 753
Bsp143I	GATC	7 cut(s)	217, 372, 427, 781, 1762, 2035, 2439
BspACI	CCGC	7 cut(s)	33, 206, 568, 709, 767, 1050, 1273
BspANI	GGCC	7 cut(s)	27, 199, 232, 612, 764, 1247, 2188
BspCNI	CTCAG	7 cut(s)	133, 247, 403, 1216, 1312, 1973, 2460
BspFNI	CGCG	1 cut(s)	568
BspLI	GGNNCC	5 cut(s)	765, 798, 969, 999, 2424
BspMAI	CTGCAG	4 cut(s)	699, 1111, 1762, 2158
BspMI	ACCTGC	1 cut(s)	1178
BspOI	GCTAGC	1 cut(s)	2258
BspPI	GGATC	4 cut(s)	380, 776, 1757, 2434
BspQI	GCTCTTC	1 cut(s)	2079
BspT104I	TTCGAA	1 cut(s)	1164
BspT107I	GGYRCC	1 cut(s)	796
BspTI	CTTAAG	1 cut(s)	1613
BspTNI	GGTCTC	1 cut(s)	2339
BsrGI	TGTACA	2 cut(s)	435, 753
BsrI	ACTGG	6 cut(s)	735, 949, 1026, 1347, 2182, 2345
BssECI	CCNNGG	2 cut(s)	465, 2145
BssMI	GATC	7 cut(s)	217, 372, 427, 781, 1762, 2035, 2439
BssSI	CACGAG	2 cut(s)	581, 840
Bst2BI	CACGAG	2 cut(s)	581, 840
Bst2UI	CCWGG	3 cut(s)	819, 2146, 2289
Bst4CI	ACNGT	7 cut(s)	86, 350, 1331, 1571, 1806, 2272, 2398
Bst6I	CTCTTC	3 cut(s)	12, 792, 2079
BstAFI	CTTAAG	1 cut(s)	1613
BstAPI	GCANNNNNTGC	1 cut(s)	721
BstAUI	TGTACA	2 cut(s)	435, 753
BstBI	TTCGAA	1 cut(s)	1164
BstC8I	GCNNGC	6 cut(s)	149, 277, 717, 726, 730, 2256
BstDEI	CTNAG	8 cut(s)	120, 234, 342, 411, 1224, 1320, 1960, 2447
BstEII	GGTNACC	2 cut(s)	1575, 2111
BstF5I	GGATG	8 cut(s)	756, 765, 814, 945, 1265, 1343, 2035, 2104
BstFNI	CGCG	1 cut(s)	568
BstH2I	RGCGCY	1 cut(s)	1436
BstHHI	GCGC	1 cut(s)	1435
BstKTI	GATC	7 cut(s)	220, 375, 430, 784, 1765, 2038, 2442
BstMAI	GTCTC	2 cut(s)	411, 2339
BstMBI	GATC	7 cut(s)	217, 372, 427, 781, 1762, 2035, 2439
BstMWI	GCNNNNNNNGC	5 cut(s)	721, 725, 929, 1850, 2264
BstNI	CCWGG	3 cut(s)	819, 2146, 2289
BstNSI	RCATGY	5 cut(s)	719, 863, 1512, 1884, 1893
BstPI	GGTNACC	2 cut(s)	1575, 2111
BstSCI	CCNGG	4 cut(s)	47, 817, 2144, 2287
BstSFI	CTRYAG	4 cut(s)	695, 1107, 1758, 2154
BstUI	CGCG	1 cut(s)	568
BstV1I	GCAGC	5 cut(s)	659, 919, 941, 1093, 2140
BstV2I	GAAGAC	2 cut(s)	1398, 1716
BstX2I	RGATCY	1 cut(s)	1762
BstXI	CCANNNNNNTGG	2 cut(s)	1011, 1448
BstYI	RGATCY	1 cut(s)	1762
BsuRI	GGCC	7 cut(s)	27, 199, 232, 612, 764, 1247, 2188
BtsCI	GGATG	8 cut(s)	756, 765, 814, 945, 1265, 1343, 2035, 2104
BtsI	GCAGTG	2 cut(s)	704, 1116
BtsIMutI	CAGTG	8 cut(s)	704, 1033, 1116, 1354, 2046, 2189, 2338, 2394
BveI	ACCTGC	1 cut(s)	1178
Cac8I	GCNNGC	6 cut(s)	149, 277, 717, 726, 730, 2256
CaiI	CAGNNNCTG	1 cut(s)	2437
CfoI	GCGC	1 cut(s)	1435
Cfr13I	GGNCC	6 cut(s)	26, 66, 610, 763, 821, 968
CseI	GACGC	3 cut(s)	451, 1025, 2364
CsiI	ACCWGGT	1 cut(s)	2287
Csp6I	GTAC	4 cut(s)	436, 754, 1997, 2179
CviAII	CATG	9 cut(s)	70, 75, 380, 716, 860, 1509, 1881, 1890, 2004
CviQI	GTAC	4 cut(s)	436, 754, 1997, 2179
DdeI	CTNAG	8 cut(s)	120, 234, 342, 411, 1224, 1320, 1960, 2447
DpnI	GATC	7 cut(s)	219, 374, 429, 783, 1764, 2037, 2441
DpnII	GATC	7 cut(s)	217, 372, 427, 781, 1762, 2035, 2439
DraI	TTTAAA	2 cut(s)	1816, 2302
DriI	GACNNNNNGTC	1 cut(s)	1573
EaeI	YGGCCR	2 cut(s)	197, 1245
Eam1104I	CTCTTC	3 cut(s)	12, 792, 2079
Eam1105I	GACNNNNNGTC	1 cut(s)	1573
EarI	CTCTTC	3 cut(s)	12, 792, 2079
Eco31I	GGTCTC	1 cut(s)	2339
Eco47I	GGWCC	3 cut(s)	66, 821, 968
Eco57I	CTGAAG	4 cut(s)	143, 923, 1728, 2157
Eco91I	GGTNACC	2 cut(s)	1575, 2111
EcoO65I	GGTNACC	2 cut(s)	1575, 2111
EcoRII	CCWGG	3 cut(s)	817, 2144, 2287
EcoT22I	ATGCAT	2 cut(s)	631, 2214
FaeI	CATG	9 cut(s)	73, 78, 383, 719, 863, 1512, 1884, 1893, 2007
FalI	AAGNNNNNCTT	4 cut(s)	1065, 1097, 1809, 1841
FaqI	GGGAC	1 cut(s)	1028
FatI	CATG	9 cut(s)	69, 74, 379, 715, 859, 1508, 1880, 1889, 2003
FauI	CCCGC	3 cut(s)	199, 702, 774
FauNDI	CATATG	1 cut(s)	2373
Fnu4HI	GCNGC	8 cut(s)	33, 648, 930, 933, 1051, 1107, 1274, 2154
FokI	GGATG	8 cut(s)	763, 772, 801, 932, 1272, 1330, 2042, 2111
Fsp4HI	GCNGC	8 cut(s)	33, 648, 930, 933, 1051, 1107, 1274, 2154
FspBI	CTAG	4 cut(s)	249, 1287, 1836, 2255
GlaI	GCGC	1 cut(s)	1434
GluI	GCNGC	8 cut(s)	33, 648, 930, 933, 1051, 1107, 1274, 2154
GsuI	CTGGAG	3 cut(s)	283, 1110, 2244
HaeII	RGCGCY	1 cut(s)	1436
HaeIII	GGCC	7 cut(s)	27, 199, 232, 612, 764, 1247, 2188
HapII	CCGG	6 cut(s)	36, 49, 64, 272, 1124, 1244
HgaI	GACGC	3 cut(s)	451, 1025, 2364
HhaI	GCGC	1 cut(s)	1435
Hin1II	CATG	9 cut(s)	73, 78, 383, 719, 863, 1512, 1884, 1893, 2007
Hin6I	GCGC	1 cut(s)	1433
HinP1I	GCGC	1 cut(s)	1433
HincII	GTYRAC	4 cut(s)	573, 689, 1378, 2104
HindII	GTYRAC	4 cut(s)	573, 689, 1378, 2104
HinfI	GANTC	6 cut(s)	836, 1226, 1460, 1754, 1820, 1970
HpaI	GTTAAC	1 cut(s)	1378
HpaII	CCGG	6 cut(s)	36, 49, 64, 272, 1124, 1244
Hpy166II	GTNNAC	9 cut(s)	89, 436, 573, 689, 824, 1378, 1454, 1934, 2104
Hpy8I	GTNNAC	9 cut(s)	89, 436, 573, 689, 824, 1378, 1454, 1934, 2104
HpyCH4III	ACNGT	7 cut(s)	86, 350, 1331, 1571, 1806, 2272, 2398
HpyF10VI	GCNNNNNNNGC	5 cut(s)	721, 725, 929, 1850, 2264
HpyF3I	CTNAG	8 cut(s)	120, 234, 342, 411, 1224, 1320, 1960, 2447
Hsp92II	CATG	9 cut(s)	73, 78, 383, 719, 863, 1512, 1884, 1893, 2007
HspAI	GCGC	1 cut(s)	1433
KspAI	GTTAAC	1 cut(s)	1378
Kzo9I	GATC	7 cut(s)	217, 372, 427, 781, 1762, 2035, 2439
LguI	GCTCTTC	1 cut(s)	2079
LmnI	GCTCC	4 cut(s)	302, 997, 2263, 2422
Lsp1109I	GCAGC	5 cut(s)	659, 919, 941, 1093, 2140
LweI	GCATC	5 cut(s)	322, 616, 2199, 2221, 2254
MabI	ACCWGGT	1 cut(s)	2287
MaeI	CTAG	4 cut(s)	249, 1287, 1836, 2255
MaeIII	GTNAC	8 cut(s)	80, 658, 1111, 1309, 1575, 1806, 1870, 2111
MalI	GATC	7 cut(s)	219, 374, 429, 783, 1764, 2037, 2441
MboI	GATC	7 cut(s)	217, 372, 427, 781, 1762, 2035, 2439
MflI	RGATCY	1 cut(s)	1762
MhlI	GDGCHC	2 cut(s)	299, 387
MlsI	TGGCCA	1 cut(s)	199
MluNI	TGGCCA	1 cut(s)	199
MlyI	GAGTC	2 cut(s)	830, 1235
MmeI	TCCRAC	4 cut(s)	1209, 1583, 1686, 2076
Mox20I	TGGCCA	1 cut(s)	199
Mph1103I	ATGCAT	2 cut(s)	631, 2214
MscI	TGGCCA	1 cut(s)	199
MslI	CAYNNNNRTG	3 cut(s)	380, 908, 1446
Msp20I	TGGCCA	1 cut(s)	199
MspA1I	CMGCKG	2 cut(s)	932, 2451
MspCI	CTTAAG	1 cut(s)	1613
MspI	CCGG	6 cut(s)	36, 49, 64, 272, 1124, 1244
MspR9I	CCNGG	4 cut(s)	49, 819, 2146, 2289
Mva1269I	GAATGC	3 cut(s)	483, 769, 2317
MvaI	CCWGG	3 cut(s)	819, 2146, 2289
MvnI	CGCG	1 cut(s)	568
MwoI	GCNNNNNNNGC	5 cut(s)	721, 725, 929, 1850, 2264
NciI	CCSGG	1 cut(s)	49
NdeI	CATATG	1 cut(s)	2373
NdeII	GATC	7 cut(s)	217, 372, 427, 781, 1762, 2035, 2439
NheI	GCTAGC	1 cut(s)	2254
NlaIII	CATG	9 cut(s)	73, 78, 383, 719, 863, 1512, 1884, 1893, 2007
NlaIV	GGNNCC	5 cut(s)	765, 798, 969, 999, 2424
NmuCI	GTSAC	6 cut(s)	80, 658, 1111, 1309, 1575, 1870
NsiI	ATGCAT	2 cut(s)	631, 2214
NspI	RCATGY	5 cut(s)	719, 863, 1512, 1884, 1893
NspV	TTCGAA	1 cut(s)	1164
OliI	CACNNNNGTG	2 cut(s)	380, 1446
PaeI	GCATGC	1 cut(s)	719
PaqCI	CACCTGC	1 cut(s)	1178
PciI	ACATGT	3 cut(s)	1508, 1880, 1889
PciSI	GCTCTTC	1 cut(s)	2079
PctI	GAATGC	3 cut(s)	483, 769, 2317
PfeI	GAWTC	4 cut(s)	1460, 1754, 1820, 1970
PflMI	CCANNNNNTGG	1 cut(s)	75
PkrI	GCNGC	8 cut(s)	34, 649, 931, 934, 1052, 1108, 1275, 2155
PleI	GAGTC	2 cut(s)	830, 1234
PpsI	GAGTC	2 cut(s)	830, 1234
PscI	ACATGT	3 cut(s)	1508, 1880, 1889
Psp6I	CCWGG	3 cut(s)	817, 2144, 2287
PspEI	GGTNACC	2 cut(s)	1575, 2111
PspGI	CCWGG	3 cut(s)	817, 2144, 2287
PspN4I	GGNNCC	5 cut(s)	765, 798, 969, 999, 2424
PspPI	GGNCC	6 cut(s)	26, 66, 610, 763, 821, 968
PstI	CTGCAG	4 cut(s)	699, 1111, 1762, 2158
PstNI	CAGNNNCTG	1 cut(s)	2437
PsuI	RGATCY	1 cut(s)	1762
PvuII	CAGCTG	2 cut(s)	932, 2451
RsaI	GTAC	4 cut(s)	437, 755, 1998, 2180
RsaNI	GTAC	4 cut(s)	436, 754, 1997, 2179
RseI	CAYNNNNRTG	3 cut(s)	380, 908, 1446
SapI	GCTCTTC	1 cut(s)	2079
SatI	GCNGC	8 cut(s)	33, 648, 930, 933, 1051, 1107, 1274, 2154
Sau3AI	GATC	7 cut(s)	217, 372, 427, 781, 1762, 2035, 2439
Sau96I	GGNCC	6 cut(s)	26, 66, 610, 763, 821, 968
SchI	GAGTC	2 cut(s)	830, 1235
ScrFI	CCNGG	4 cut(s)	49, 819, 2146, 2289
SduI	GDGCHC	2 cut(s)	299, 387
SexAI	ACCWGGT	1 cut(s)	2287
SfaNI	GCATC	5 cut(s)	322, 616, 2199, 2221, 2254
SfcI	CTRYAG	4 cut(s)	695, 1107, 1758, 2154
SfuI	TTCGAA	1 cut(s)	1164
SinI	GGWCC	3 cut(s)	66, 821, 968
SmiMI	CAYNNNNRTG	3 cut(s)	380, 908, 1446
SmlI	CTYRAG	2 cut(s)	1613, 2366
SmoI	CTYRAG	2 cut(s)	1613, 2366
SphI	GCATGC	1 cut(s)	719
SsiI	CCGC	7 cut(s)	33, 206, 568, 709, 767, 1050, 1273
SspMI	CTAG	4 cut(s)	249, 1287, 1836, 2255
StyD4I	CCNGG	4 cut(s)	47, 817, 2144, 2287
TaaI	ACNGT	7 cut(s)	86, 350, 1331, 1571, 1806, 2272, 2398
TaqI	TCGA	3 cut(s)	1164, 1886, 1985
TatI	WGTACW	2 cut(s)	435, 753
TauI	GCSGC	3 cut(s)	35, 1053, 1276
TfiI	GAWTC	4 cut(s)	1460, 1754, 1820, 1970
TscAI	CASTG	8 cut(s)	704, 1033, 1116, 1354, 2053, 2189, 2345, 2401
TseFI	GTSAC	6 cut(s)	80, 658, 1111, 1309, 1575, 1870
TseI	GCWGC	5 cut(s)	647, 929, 932, 1106, 2153
Tsp45I	GTSAC	6 cut(s)	80, 658, 1111, 1309, 1575, 1870
TspDTI	ATGAA	7 cut(s)	762, 779, 1002, 1313, 1659, 2139, 2219
TspRI	CASTG	8 cut(s)	704, 1033, 1116, 1354, 2053, 2189, 2345, 2401
Van91I	CCANNNNNTGG	1 cut(s)	75
Vha464I	CTTAAG	1 cut(s)	1613
VpaK11BI	GGWCC	3 cut(s)	66, 821, 968
XapI	RAATTY	3 cut(s)	1007, 1167, 1990
XceI	RCATGY	5 cut(s)	719, 863, 1512, 1884, 1893
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1008, 1354
XspI	CTAG	4 cut(s)	249, 1287, 1836, 2255
Zsp2I	ATGCAT	2 cut(s)	631, 2214

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.