Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014721

Physical Location & Seq

Genomic Coordinates Reverse (-)

7227469 .. 7230356

Gene Structure 2888 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1103200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGTTAGGGGAAGAAGAGAAGGACCGGCTCGCCGGAAATACTACCCGGAAAACCTATCTGCGGTCGGTTTCGTGGTCTGACCGGTCACCCTCTAAGCCCAACAATCCATATCCGAGGCCACAACTGAATACTAAGGTGAGGTCTTGCCTACCTCCACTTCAGCCCCTTTCAATAGCCAGAAATACCGTTAAGGAGTGGCCACGGGCAGGTTCTGATGATCTCGGGGTCTGGCCTCAACCGCAGACCCCGAGAGGGTCAGCTAAACCGCTTCCGAACTCGAATCCAGAAGAACCTGGGAGAGAATTTGAGTTTAAGAAGGATAAGCTTGCTTTTTTCGATAAAGAGTGCTCGAGAATTGCTGATCATATATACTTGGGAAGTGATGCGGTGGCTAAGAACCGCGAGGTTTTGAGGCAGAATGGGATCACTCATGTGCTGAACTGTGTTGGGTTTGTTTCTCCCGAGTATTTCAGAAATGATCTTGTGTACAAGACACTTTGGTTGCGAGATAGCACATCGGAGGACATTACGAGTATACTATATGATGTGTTTGATTATTTTGAAGATGTTCGAAAGCAAGGCGGTCGAGTGTTTGTGCATTGTTGTCAGGGAGTGTCTCGGTCCAACTCTCTGGTCATTGCATACCTCATGTGGAGGGAGGGCCACAGCTTTGAAGATGCGTTCCAGTATGTGAAGGCAGCACGAGGGGTGACTAACCCGAATATAGGTTTTGCTTGTCAACTCCTTCAGTGCCAGAAGAGGGTACATGCTGTGCCTGCAAGCCCAAATTCCATGTTAAGGATGTATCGGATGGCCCCACATTCACCATATGATCCTCTTCATCTGGTGCCAAAGACGTTGGGACATCCAGGTTCACAAGGACTCGACTCTCGTGGGGCGTTCGTTGTGCATGTTCCATCTGCTATATACGTCTGGATTGGAAAGGATTGCAACACAAGGATGTCAGATAATGCCAGGGCAGCTGCCTTTCAGGTCATCCGGTATGAGAGGGCAAGGGGTCCAATTGTGGACATCAATGAAGGTGAAGAACCGTTGGAGTTTTGGGATGCTCTTTCCAGTAGAATATTAGCAGAAGATTCCAGCAAGATGGATGCCAAAAAGGTAGAAACTTTTTCTTCCGCAGGTGATAAGGTTTCTGCAGCGAGCTGTGTTCCGGTGGGTGAAAGGAAGGTTGCTGAATATGATTTGGATTTCGAAATTTTCCATAAGGCACTTGCAGGTGGGGTTGTTCCACCTTTTTCAGTATCAAATACCGAATCAGAAACTTGCCTTCCAGCCAGAGAAAATGGATGGGGTAGATTGCGTCAAAAGTTTGCCAGCGGAATTATGAAAGAATTGATCACATCTGAGGTGAACCGTAACCCTACCCCATCTGATGTATCTGATATGGTTGTGGAAATTCATAAAGAAGCAGAAACCTCTGTTTCCCCGATTGAGCCTTCATCGCCATTATCAGCATCACGTCATTTTTGTGGTTCACCAGATTCCTTTGAATGTTATCCAAATAAAAGCCCACGTAGGGTAACAGATACTTTTAGAGAGGTAGAATGCTTCGTTCCTCTAACCGATAAGTTACTGTTACCTGCAACTCTTTGTGGTTCACCAGATTCTTTTTCTTGTTTTCCTGACAGAAGTCCTAAGTTTAGCTCCAAATCCCCAACACTCTCTCCTTCAACCTCTGATTACTCCAGTTCATTTACCTTTTCACCCTCATCTTCTAATTGGTCGGACTTGTCATATTTGTCTTCTCGACAGCCTTCACCTTCTGGCTTCGATTCCACAGATCCATCTCATGTTAAGAATATTTCTTTGGCAGATAACTCAAGCTTACTTTTCAAAAAATCTCCCTCTTCGCCTACAGAGGCCTTTTCGCCTAATTGTACTTTGGAAGTGACAAATACAGGCTTGCCATGTAAGGGGATTTCCCCCTCTATTGCGGAGCGTAGAGGCAGTAATCCTCCACCTCGAATGTTGGTACCTCCGGTAGATGAATCACCGAAAGTTCCGAGTAATCTGGTACGCTCATGGTCCTTCTCCTTACCTGACTTGGGTGATGATGCAATGGACACTGATTGCAACCAATCTGAAAATGGGAACAACGGAGAAGAGCTTATGTTAGATGTGGACAGTAGTCATCATGGAAATGATTTGAAATGTGAAAGGATCACGCAAGCTGCAGAAGCGATCAACCCAGTTTTGTACCAGTGGCCTTCTTTGAATAAAGTGGAGATGCATCGGTCTCACAAACTTCATTCTGGATCAGCGTATCTTTTGCCAGCTCCAGATATGAGTGCAGGCGCAAGTAATCCTGGTATCTTATTTGTCTGGTTGGGACGCGAAGTTTTGCATGAGGAAGGGCAAAGTCCGACATGTGAGGATAGACATCTTCACTGGGAGGCCACAGGACACAACTTCCTTGATCGCATGGGTTTGCCGATGAATGCCCCTGTACAGATAGTAAGAGAAGGTGAGGAGCCAGAGCAGTTCCTGATCCATCTCAGCCGTATATCAATACAGGAAACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000188 GO:0001704 GO:0001706 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007369 GO:0007492 GO:0008150 GO:0008152 GO:0008330 GO:0009653 GO:0009790 GO:0009888 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033549 GO:0033673 GO:0035335 GO:0035970 GO:0036211 GO:0042325 GO:0042326 GO:0042578 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043407 GO:0043408 GO:0043409 GO:0043412 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045936 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048598 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0070372 GO:0070373 GO:0071704 GO:0071900 GO:0071901 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902532 GO:1990439

KEGG Pathways

Metabolic & Signaling

ko04010 ko04726 ko05418 map04010 map04726 map05418

Pfam Domains

Protein Families

DSPc Gelsolin

Protein Analysis

849

Amino Acids

94.1

Weight (kDa)

5.8

Isoelectric Point (pI)

59.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	123 - 253	1.7e-27	Dual specificity phosphatase, catalytic domain
MPK1_gelsolin_C	PF25466	742 - 840	5.4e-29	MPK1 gelsolin domain C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014721)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25430 FvH4_4g25430
malus_domestica	MD13G1103000.v1.1 MD16G1103200.v1.1
prunus_persica	Prupe.1G242800_v2.0.a1
pyrus_communis	pycom16g08800
rosa_chinensis	RchiOBHm_Chr4g0432741
rosa_laevigata	RLG00000006816
rosa_multiflora	Rmu_co8494851.1_g000001 Rmu_sc0012180.1_g000009
rosa_roxburghii	Rroxscaffold_5G00374200
rosa_rugosa	Rorug04G0262100
rosa_samantha	Rh4AG316600 Rh4BG324700 Rh4CG339700 Rh4DG319900
rosa_wichuraiana	Rw4G027540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1133, 1229
Acc36I	ACCTGC	4 cut(s)	197, 1133, 1229, 1612
Acc65I	GGTACC	1 cut(s)	1996
AccB1I	GGYRCC	2 cut(s)	847, 1996
AccI	GTMKAC	1 cut(s)	535
AccII	CGCG	2 cut(s)	402, 2358
AciI	CCGC	9 cut(s)	61, 239, 266, 386, 400, 582, 1140, 1341, 1958
AclWI	GGATC	6 cut(s)	431, 827, 1799, 2192, 2287, 2506
AcoI	YGGCCR	1 cut(s)	197
AcsI	RAATTY	4 cut(s)	302, 787, 1218, 1419
AcuI	CTGAAG	2 cut(s)	143, 731
AfaI	GTAC	7 cut(s)	488, 765, 1903, 1998, 2040, 2222, 2472
AfiI	CCNNNNNNNGG	9 cut(s)	60, 206, 252, 725, 760, 798, 1540, 1937, 2069
AflIII	ACRYGT	1 cut(s)	2390
AgeI	ACCGGT	1 cut(s)	81
AgsI	TTSAA	8 cut(s)	171, 563, 674, 1514, 1695, 1858, 2173, 2239
AjiI	CACGTC	1 cut(s)	1484
AjnI	CCWGG	4 cut(s)	292, 868, 974, 2329
AjuI	GAANNNNNNNTTGG	2 cut(s)	1814, 1846
AleI	CACNNNNGTG	1 cut(s)	431
Alw21I	GWGCWC	1 cut(s)	350
Alw26I	GTCTC	2 cut(s)	621, 2265
AlwI	GGATC	6 cut(s)	431, 827, 1799, 2192, 2287, 2506
AlwNI	CAGNNNCTG	2 cut(s)	212, 2509
Ama87I	CYCGRG	4 cut(s)	221, 247, 349, 461
AoxI	GGCC	8 cut(s)	116, 197, 230, 661, 813, 1884, 2228, 2418
ApeKI	GCWGC	5 cut(s)	698, 980, 983, 1160, 2195
ApoI	RAATTY	4 cut(s)	302, 787, 1218, 1419
AsiGI	ACCGGT	1 cut(s)	81
Asp700I	GAANNNNTTC	1 cut(s)	567
Asp718I	GGTACC	1 cut(s)	1996
AspLEI	GCGC	1 cut(s)	2321
AspS9I	GGNCC	6 cut(s)	22, 621, 661, 814, 1019, 2049
AsuC2I	CCSGG	1 cut(s)	46
AsuII	TTCGAA	2 cut(s)	571, 1215
AvaI	CYCGRG	4 cut(s)	221, 247, 349, 461
AvaII	GGWCC	4 cut(s)	22, 621, 1019, 2049
BalI	TGGCCA	1 cut(s)	199
BanI	GGYRCC	2 cut(s)	847, 1996
BauI	CACGAG	2 cut(s)	702, 891
BbsI	GAAGAC	1 cut(s)	1758
Bbv12I	GWGCWC	1 cut(s)	350
BbvI	GCAGC	5 cut(s)	710, 970, 992, 1172, 2182
BccI	CCATC	7 cut(s)	805, 925, 1102, 1305, 1399, 1816, 2523
BceAI	ACGGC	1 cut(s)	2508
BcgI	CGANNNNNNTGC	2 cut(s)	566, 600
BciT130I	CCWGG	4 cut(s)	294, 870, 976, 2331
BclI	TGATCA	2 cut(s)	361, 1359
BcnI	CCSGG	1 cut(s)	46
BcoDI	GTCTC	2 cut(s)	621, 2265
BfmI	CTRYAG	3 cut(s)	1158, 1878, 2196
BfuAI	ACCTGC	4 cut(s)	197, 1133, 1229, 1612
BisI	GCNGC	5 cut(s)	699, 981, 984, 1161, 2196
BlsI	GCNGC	5 cut(s)	700, 982, 985, 1162, 2197
Bme1390I	CCNGG	5 cut(s)	46, 294, 870, 976, 2331
Bme18I	GGWCC	4 cut(s)	22, 621, 1019, 2049
BmeT110I	CYCGRG	4 cut(s)	221, 247, 349, 461
BmgBI	CACGTC	1 cut(s)	1484
BmgT120I	GGNCC	6 cut(s)	22, 621, 661, 814, 1019, 2049
BmiI	GGNNCC	5 cut(s)	816, 849, 1020, 1998, 2496
BmrFI	CCNGG	5 cut(s)	46, 294, 870, 976, 2331
BmrI	ACTGGG	2 cut(s)	2207, 2422
BmsI	GCATC	8 cut(s)	373, 667, 1057, 1102, 1487, 2068, 2241, 2263
BmuI	ACTGGG	2 cut(s)	2207, 2422
BoxI	GACNNNNGTC	2 cut(s)	1653, 2151
BpiI	GAAGAC	1 cut(s)	1758
BpmI	CTGGAG	2 cut(s)	1693, 2286
Bpu14I	TTCGAA	2 cut(s)	571, 1215
BpuEI	CTTGAG	1 cut(s)	1828
BpuMI	CCSGG	1 cut(s)	46
BsaAI	YACGTR	1 cut(s)	1538
BsaBI	GATNNNNATC	1 cut(s)	2403
BsaI	GGTCTC	1 cut(s)	2265
BsaJI	CCNNGG	4 cut(s)	113, 200, 293, 975
BsaWI	WCCGGW	4 cut(s)	81, 999, 1174, 2002
BsaXI	ACNNNNNCTCC	2 cut(s)	1672, 1702
Bsc4I	CCNNNNNNNGG	9 cut(s)	60, 206, 252, 725, 760, 798, 1540, 1937, 2069
Bse118I	RCCGGY	2 cut(s)	24, 81
Bse1I	ACTGG	6 cut(s)	685, 1077, 1710, 2213, 2224, 2417
Bse3DI	GCAATG	2 cut(s)	636, 2088
Bse8I	GATNNNNATC	1 cut(s)	2403
BseBI	CCWGG	4 cut(s)	294, 870, 976, 2331
BseDI	CCNNGG	4 cut(s)	113, 200, 293, 975
BseGI	GGATG	8 cut(s)	807, 816, 865, 966, 996, 1072, 1117, 1316
BseJI	GATNNNNATC	1 cut(s)	2403
BseLI	CCNNNNNNNGG	9 cut(s)	60, 206, 252, 725, 760, 798, 1540, 1937, 2069
BseMI	GCAATG	2 cut(s)	636, 2088
BseMII	CTCAG	2 cut(s)	1359, 2533
BseNI	ACTGG	6 cut(s)	685, 1077, 1710, 2213, 2224, 2417
BseRI	GAGGAG	1 cut(s)	2507
BseXI	GCAGC	5 cut(s)	710, 970, 992, 1172, 2182
BsgI	GTGCAG	1 cut(s)	2334
Bsh1236I	CGCG	2 cut(s)	402, 2358
Bsh1285I	CGRYCG	2 cut(s)	65, 586
BshFI	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1886, 2230, 2420
BshNI	GGYRCC	2 cut(s)	847, 1996
BshTI	ACCGGT	1 cut(s)	81
BsiEI	CGRYCG	2 cut(s)	65, 586
BsiHKAI	GWGCWC	1 cut(s)	350
BsiHKCI	CYCGRG	4 cut(s)	221, 247, 349, 461
BsiSI	CCGG	7 cut(s)	25, 33, 46, 82, 1000, 1175, 2003
BslFI	GGGAC	2 cut(s)	876, 2367
BslI	CCNNNNNNNGG	9 cut(s)	60, 206, 252, 725, 760, 798, 1540, 1937, 2069
BsmAI	GTCTC	2 cut(s)	621, 2265
BsmFI	GGGAC	2 cut(s)	876, 2367
BsmI	GAATGC	2 cut(s)	1574, 2467
BsnI	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1886, 2230, 2420
Bso31I	GGTCTC	1 cut(s)	2265
BsoBI	CYCGRG	4 cut(s)	221, 247, 349, 461
Bsp119I	TTCGAA	2 cut(s)	571, 1215
Bsp1286I	GDGCHC	1 cut(s)	350
Bsp1407I	TGTACA	2 cut(s)	486, 2470
BspACI	CCGC	9 cut(s)	61, 239, 266, 386, 400, 582, 1140, 1341, 1958
BspANI	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1886, 2230, 2420
BspCNI	CTCAG	2 cut(s)	1360, 2532
BspFNI	CGCG	2 cut(s)	402, 2358
BspLI	GGNNCC	5 cut(s)	816, 849, 1020, 1998, 2496
BspMAI	CTGCAG	2 cut(s)	1162, 2200
BspMI	ACCTGC	4 cut(s)	197, 1133, 1229, 1612
BspPI	GGATC	6 cut(s)	431, 827, 1799, 2192, 2287, 2506
BspQI	GCTCTTC	1 cut(s)	2121
BspT104I	TTCGAA	2 cut(s)	571, 1215
BspT107I	GGYRCC	2 cut(s)	847, 1996
BspTNI	GGTCTC	1 cut(s)	2265
BsrDI	GCAATG	2 cut(s)	636, 2088
BsrFI	RCCGGY	2 cut(s)	24, 81
BsrGI	TGTACA	2 cut(s)	486, 2470
BsrI	ACTGG	6 cut(s)	685, 1077, 1710, 2213, 2224, 2417
BssAI	RCCGGY	2 cut(s)	24, 81
BssECI	CCNNGG	4 cut(s)	113, 200, 293, 975
BssNAI	GTATAC	1 cut(s)	536
BssSI	CACGAG	2 cut(s)	702, 891
Bst1107I	GTATAC	1 cut(s)	536
Bst2BI	CACGAG	2 cut(s)	702, 891
Bst2UI	CCWGG	4 cut(s)	294, 870, 976, 2331
Bst4CI	ACNGT	6 cut(s)	187, 443, 1053, 1379, 1599, 2150
Bst6I	CTCTTC	5 cut(s)	9, 752, 843, 1876, 2121
BstAUI	TGTACA	2 cut(s)	486, 2470
BstBAI	YACGTR	1 cut(s)	1538
BstBI	TTCGAA	2 cut(s)	571, 1215
BstDEI	CTNAG	6 cut(s)	93, 132, 393, 1368, 1659, 2519
BstDSI	CCRYGG	1 cut(s)	200
BstEII	GGTNACC	1 cut(s)	84
BstF5I	GGATG	8 cut(s)	807, 816, 865, 966, 996, 1072, 1117, 1316
BstFNI	CGCG	2 cut(s)	402, 2358
BstHHI	GCGC	1 cut(s)	2321
BstMAI	GTCTC	2 cut(s)	621, 2265
BstMCI	CGRYCG	2 cut(s)	65, 586
BstMWI	GCNNNNNNNGC	5 cut(s)	238, 776, 980, 1465, 2201
BstNI	CCWGG	4 cut(s)	294, 870, 976, 2331
BstNSI	RCATGY	3 cut(s)	770, 914, 2394
BstPAI	GACNNNNGTC	2 cut(s)	1653, 2151
BstPI	GGTNACC	1 cut(s)	84
BstSCI	CCNGG	5 cut(s)	44, 292, 868, 974, 2329
BstSFI	CTRYAG	3 cut(s)	1158, 1878, 2196
BstUI	CGCG	2 cut(s)	402, 2358
BstV1I	GCAGC	5 cut(s)	710, 970, 992, 1172, 2182
BstV2I	GAAGAC	1 cut(s)	1758
BstX2I	RGATCY	1 cut(s)	1804
BstXI	CCANNNNNNTGG	2 cut(s)	631, 1108
BstYI	RGATCY	1 cut(s)	1804
BstZ17I	GTATAC	1 cut(s)	536
BsuRI	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1886, 2230, 2420
BtgI	CCRYGG	1 cut(s)	200
BtgZI	GCGATG	1 cut(s)	1449
BtrI	CACGTC	1 cut(s)	1484
BtsCI	GGATG	8 cut(s)	807, 816, 865, 966, 996, 1072, 1117, 1316
BtsIMutI	CAGTG	4 cut(s)	755, 2088, 2231, 2410
BveI	ACCTGC	4 cut(s)	197, 1133, 1229, 1612
CaiI	CAGNNNCTG	2 cut(s)	212, 2509
CfoI	GCGC	1 cut(s)	2321
Cfr10I	RCCGGY	2 cut(s)	24, 81
Cfr13I	GGNCC	6 cut(s)	22, 621, 661, 814, 1019, 2049
CseI	GACGC	2 cut(s)	1313, 2364
Csp6I	GTAC	7 cut(s)	487, 764, 1902, 1997, 2039, 2221, 2471
CspAI	ACCGGT	1 cut(s)	81
CviQI	GTAC	7 cut(s)	487, 764, 1902, 1997, 2039, 2221, 2471
DdeI	CTNAG	6 cut(s)	93, 132, 393, 1368, 1659, 2519
EaeI	YGGCCR	1 cut(s)	197
Eam1104I	CTCTTC	5 cut(s)	9, 752, 843, 1876, 2121
EarI	CTCTTC	5 cut(s)	9, 752, 843, 1876, 2121
Eco147I	AGGCCT	1 cut(s)	1886
Eco31I	GGTCTC	1 cut(s)	2265
Eco47I	GGWCC	4 cut(s)	22, 621, 1019, 2049
Eco57I	CTGAAG	2 cut(s)	143, 731
Eco88I	CYCGRG	4 cut(s)	221, 247, 349, 461
Eco91I	GGTNACC	1 cut(s)	84
EcoO65I	GGTNACC	1 cut(s)	84
EcoRII	CCWGG	4 cut(s)	292, 868, 974, 2329
EcoT22I	ATGCAT	1 cut(s)	2256
FaqI	GGGAC	2 cut(s)	876, 2367
FauNDI	CATATG	1 cut(s)	829
FbaI	TGATCA	2 cut(s)	361, 1359
FblI	GTMKAC	1 cut(s)	535
Fnu4HI	GCNGC	5 cut(s)	699, 981, 984, 1161, 2196
FokI	GGATG	8 cut(s)	814, 823, 852, 973, 983, 1079, 1124, 1323
Fsp4HI	GCNGC	5 cut(s)	699, 981, 984, 1161, 2196
GlaI	GCGC	1 cut(s)	2320
GluI	GCNGC	5 cut(s)	699, 981, 984, 1161, 2196
GsuI	CTGGAG	2 cut(s)	1693, 2286
HaeIII	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1886, 2230, 2420
HapII	CCGG	7 cut(s)	25, 33, 46, 82, 1000, 1175, 2003
HgaI	GACGC	2 cut(s)	1313, 2364
HhaI	GCGC	1 cut(s)	2321
Hin6I	GCGC	1 cut(s)	2319
HinP1I	GCGC	1 cut(s)	2319
HincII	GTYRAC	1 cut(s)	740
HindII	GTYRAC	1 cut(s)	740
HindIII	AAGCTT	2 cut(s)	323, 1846
HinfI	GANTC	9 cut(s)	280, 882, 887, 1097, 1277, 1505, 1628, 1796, 2012
HpaII	CCGG	7 cut(s)	25, 33, 46, 82, 1000, 1175, 2003
Hpy166II	GTNNAC	9 cut(s)	487, 536, 740, 875, 1030, 1375, 1499, 1622, 2146
Hpy188III	TCNNGA	9 cut(s)	284, 351, 461, 934, 1646, 1770, 2277, 2303, 2509
Hpy8I	GTNNAC	9 cut(s)	487, 536, 740, 875, 1030, 1375, 1499, 1622, 2146
HpyCH4III	ACNGT	6 cut(s)	187, 443, 1053, 1379, 1599, 2150
HpyCH4IV	ACGT	5 cut(s)	857, 930, 1483, 1537, 2543
HpyF10VI	GCNNNNNNNGC	5 cut(s)	238, 776, 980, 1465, 2201
HpyF3I	CTNAG	6 cut(s)	93, 132, 393, 1368, 1659, 2519
HpySE526I	ACGT	5 cut(s)	857, 930, 1483, 1537, 2543
HspAI	GCGC	1 cut(s)	2319
KpnI	GGTACC	1 cut(s)	2000
Ksp22I	TGATCA	2 cut(s)	361, 1359
LguI	GCTCTTC	1 cut(s)	2121
LmnI	GCTCC	4 cut(s)	1673, 1960, 2305, 2494
Lsp1109I	GCAGC	5 cut(s)	710, 970, 992, 1172, 2182
LweI	GCATC	8 cut(s)	373, 667, 1057, 1102, 1487, 2068, 2241, 2263
MaeII	ACGT	5 cut(s)	857, 930, 1483, 1537, 2543
MaeIII	GTNAC	7 cut(s)	84, 709, 1379, 1543, 1593, 1599, 1912
MfeI	CAATTG	1 cut(s)	1023
MflI	RGATCY	1 cut(s)	1804
MhlI	GDGCHC	1 cut(s)	350
MlsI	TGGCCA	1 cut(s)	199
MluNI	TGGCCA	1 cut(s)	199
MlyI	GAGTC	2 cut(s)	876, 881
MmeI	TCCRAC	4 cut(s)	648, 1035, 1728, 2411
Mox20I	TGGCCA	1 cut(s)	199
Mph1103I	ATGCAT	1 cut(s)	2256
MroXI	GAANNNNTTC	1 cut(s)	567
MscI	TGGCCA	1 cut(s)	199
MseI	TTAA	4 cut(s)	189, 312, 797, 1818
MslI	CAYNNNNRTG	4 cut(s)	431, 959, 1491, 2163
Msp20I	TGGCCA	1 cut(s)	199
MspA1I	CMGCKG	2 cut(s)	983, 1341
MspI	CCGG	7 cut(s)	25, 33, 46, 82, 1000, 1175, 2003
MspR9I	CCNGG	5 cut(s)	46, 294, 870, 976, 2331
MunI	CAATTG	1 cut(s)	1023
Mva1269I	GAATGC	2 cut(s)	1574, 2467
MvaI	CCWGG	4 cut(s)	294, 870, 976, 2331
MvnI	CGCG	2 cut(s)	402, 2358
MwoI	GCNNNNNNNGC	5 cut(s)	238, 776, 980, 1465, 2201
NciI	CCSGG	1 cut(s)	46
NdeI	CATATG	1 cut(s)	829
NlaIV	GGNNCC	5 cut(s)	816, 849, 1020, 1998, 2496
NmuCI	GTSAC	3 cut(s)	84, 709, 1912
NsiI	ATGCAT	1 cut(s)	2256
NspI	RCATGY	3 cut(s)	770, 914, 2394
NspV	TTCGAA	2 cut(s)	571, 1215
OliI	CACNNNNGTG	1 cut(s)	431
PaeR7I	CTCGAG	1 cut(s)	349
PaqCI	CACCTGC	2 cut(s)	1133, 1229
PceI	AGGCCT	1 cut(s)	1886
PciI	ACATGT	1 cut(s)	2390
PciSI	GCTCTTC	1 cut(s)	2121
PctI	GAATGC	2 cut(s)	1574, 2467
PdmI	GAANNNNTTC	1 cut(s)	567
PfeI	GAWTC	7 cut(s)	280, 1097, 1277, 1505, 1628, 1796, 2012
PinAI	ACCGGT	1 cut(s)	81
PkrI	GCNGC	5 cut(s)	700, 982, 985, 1162, 2197
PleI	GAGTC	2 cut(s)	876, 881
PpsI	GAGTC	2 cut(s)	876, 881
Ppu21I	YACGTR	1 cut(s)	1538
PscI	ACATGT	1 cut(s)	2390
PshAI	GACNNNNGTC	2 cut(s)	1653, 2151
Psp6I	CCWGG	4 cut(s)	292, 868, 974, 2329
PspEI	GGTNACC	1 cut(s)	84
PspGI	CCWGG	4 cut(s)	292, 868, 974, 2329
PspN4I	GGNNCC	5 cut(s)	816, 849, 1020, 1998, 2496
PspPI	GGNCC	6 cut(s)	22, 621, 661, 814, 1019, 2049
PstI	CTGCAG	2 cut(s)	1162, 2200
PstNI	CAGNNNCTG	2 cut(s)	212, 2509
PsuI	RGATCY	1 cut(s)	1804
PvuII	CAGCTG	1 cut(s)	983
RsaI	GTAC	7 cut(s)	488, 765, 1903, 1998, 2040, 2222, 2472
RsaNI	GTAC	7 cut(s)	487, 764, 1902, 1997, 2039, 2221, 2471
RseI	CAYNNNNRTG	4 cut(s)	431, 959, 1491, 2163
SapI	GCTCTTC	1 cut(s)	2121
SaqAI	TTAA	4 cut(s)	189, 312, 797, 1818
SatI	GCNGC	5 cut(s)	699, 981, 984, 1161, 2196
Sau96I	GGNCC	6 cut(s)	22, 621, 661, 814, 1019, 2049
SchI	GAGTC	2 cut(s)	876, 881
ScrFI	CCNGG	5 cut(s)	46, 294, 870, 976, 2331
SduI	GDGCHC	1 cut(s)	350
SfaNI	GCATC	8 cut(s)	373, 667, 1057, 1102, 1487, 2068, 2241, 2263
SfcI	CTRYAG	3 cut(s)	1158, 1878, 2196
Sfr274I	CTCGAG	1 cut(s)	349
SfuI	TTCGAA	2 cut(s)	571, 1215
SinI	GGWCC	4 cut(s)	22, 621, 1019, 2049
SlaI	CTCGAG	1 cut(s)	349
SmiMI	CAYNNNNRTG	4 cut(s)	431, 959, 1491, 2163
SmlI	CTYRAG	2 cut(s)	349, 1843
SmoI	CTYRAG	2 cut(s)	349, 1843
SseBI	AGGCCT	1 cut(s)	1886
SsiI	CCGC	9 cut(s)	61, 239, 266, 386, 400, 582, 1140, 1341, 1958
SspI	AATATT	2 cut(s)	1086, 1825
StuI	AGGCCT	1 cut(s)	1886
StyD4I	CCNGG	5 cut(s)	44, 292, 868, 974, 2329
TaaI	ACNGT	6 cut(s)	187, 443, 1053, 1379, 1599, 2150
TaiI	ACGT	5 cut(s)	860, 933, 1486, 1540, 2546
TaqII	GACCGA	2 cut(s)	609, 2247
TatI	WGTACW	3 cut(s)	486, 1901, 2470
TfiI	GAWTC	7 cut(s)	280, 1097, 1277, 1505, 1628, 1796, 2012
Tru1I	TTAA	4 cut(s)	189, 312, 797, 1818
Tru9I	TTAA	4 cut(s)	189, 312, 797, 1818
TscAI	CASTG	4 cut(s)	755, 2095, 2231, 2417
TseFI	GTSAC	3 cut(s)	84, 709, 1912
TseI	GCWGC	5 cut(s)	698, 980, 983, 1160, 2195
Tsp45I	GTSAC	3 cut(s)	84, 709, 1912
TspDTI	ATGAA	9 cut(s)	830, 1053, 1364, 1412, 1452, 1704, 2025, 2261, 2474
TspGWI	ACGGA	1 cut(s)	2136
TspRI	CASTG	4 cut(s)	755, 2095, 2231, 2417
VpaK11BI	GGWCC	4 cut(s)	22, 621, 1019, 2049
XapI	RAATTY	4 cut(s)	302, 787, 1218, 1419
XceI	RCATGY	3 cut(s)	770, 914, 2394
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2108
XhoI	CTCGAG	1 cut(s)	349
XmiI	GTMKAC	1 cut(s)	535
XmnI	GAANNNNTTC	1 cut(s)	567
Zsp2I	ATGCAT	1 cut(s)	2256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.