Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014209

Physical Location & Seq

Genomic Coordinates Reverse (-)

7551592 .. 7552905

Gene Structure 1314 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1105300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGAGGTTCCGATCCTGGCTAGCCATCTCCTCCACCACCCGCCACTCCACCGTCCCATTCTCCAACCCTGAGACGTCACCGTCTCGGGCGAAACTCAAACCCTCCGATACCAGCAGCTCCTCCGATACTCCCAGCACTAGTACCAGCGCAAGCGACAACGGCAGTAGCAGCCTCCAAAGCAACCTCTCCCTCCAGACGCTGCCATCTGTCCTCTCCCTCCAGAAACTCTCCCCTCTAGACCCCCTCGACCTCTCCGTCTCCCACCTCTGCTTCGCCACTCTCCCGCCCCGCCGTCCCTCCCTCCCAATCACATGCCTCGCCGTCCACCACAACCTCCTCTACGCCGCATCGGGCCACGAGATCAACGTCTACGATCGTACGGACCACACGCTTCTCGATTCATTCAACGCCCACGATGCCTCCTCGGGATCCGTTAAGTCCGTTAATTTTTCCGACGGTAATATCTTCACTTCGCACCAGGACTCCAAAATCCGAGTCTGGCAGTTGACGGCGGGAAAACAGCACAAGCTTTTCAACTCCCTCCCCACCGTCAACGACCGTTTGCGCCGCTTCGTCCTCCCCAAGAACTACGTGACCGTCCGCCGCCACAAGAAATGCCTCTGGATCCAACACGCTGACGCCGTCACCGGCATCGCCGTCAACAACGGCCTGATTTACTCAGTATCCTGGGACAAGAGCCTGAAGATTTGGCGGGACTCGGATCTCCGCTGCGTCGAGTCAGTCAAGGCGCACGAGGACGCCGTGAACGCAGTGGTGGTGTCGAACGACGGGACGGTGTACACCGGGTCGGCGGATTGTCGGATCCGGGTGTGGTCCAAACCGTTCGGGGAGAAGCGCCACGTGTTGGTGGCGACGCTGGAGAAGCACAAGTCGGCAGTGAACGCTTTGGCCATAAACGACGACGGATCGGTGCTGTTTTCGGGGGCTTGCGACCGTTCAATCCTGGTGTGGGAGAGGGAGGACAGCGCCAACCATATGGCGGTGACGGGGGCGCTGAGAGGTCACCGGAAGGCTATACTGTGTATGATCAACGTCGGGGATTTGTTGTTCAGTGGGTCGGCTGATCGGACGGTGAGGGTGTGGCAACGCGGCGAGGACGGGAGTTTTTGTTGTTTGGCGGTTTTGGAGGGGCACGTGAAGCCGGTGAAGTCGTTAGTGGCGGTTAAAGAGGAGCAGTCGAACGGCGTCGTTACGGTTTACAGTGGGAGCTTGGATGGAGAAGTGAAGGTGTGGCACGTCTCGGTTTCGAATGTGAACAGTACGCCGGGCCCACTGTCTCAGTTGAAAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007049 GO:0008092 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016905 GO:0017022 GO:0017076 GO:0018107 GO:0018193 GO:0018210 GO:0019538 GO:0022402 GO:0022411 GO:0030029 GO:0030036 GO:0030554 GO:0030863 GO:0030864 GO:0031033 GO:0031035 GO:0031037 GO:0031038 GO:0032153 GO:0032155 GO:0032553 GO:0032555 GO:0032559 GO:0032984 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045159 GO:0046777 GO:0051301 GO:0061640 GO:0070938 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0099568 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

438

Amino Acids

47.78

Weight (kDa)

8.99

Isoelectric Point (pI)

42.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_2nd	PF25172	90 - 372	1.2e-19	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	210 - 279	9e-08	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	210 - 289	1.4e-09	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	211 - 370	1.9e-12	WDHD1 first WD40 domain
NBCH_WD40	PF20426	218 - 279	5.1e-07	Neurobeachin beta propeller domain
WD40_Prp19	PF24814	220 - 306	1.4e-09	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	223 - 349	1.2e-08	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	223 - 421	1.5e-10	WDR55
WD40_CDC20-Fz	PF24807	230 - 336	5.4e-10	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	240 - 429	2.8e-15	TEP-1 second beta-propeller
WD40	PF00400	243 - 279	1.5e-06	WD domain, G-beta repeat
Beta-prop_EML	PF23409	252 - 329	3.3e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR75_1st	PF23869	261 - 330	7.5e-07	WD repeat-containing protein 75 first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	264 - 386	9.3e-08	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	269 - 378	2.7e-06	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	289 - 424	2e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	289 - 425	1.8e-18	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	289 - 420	1.1e-17	WDR3 first beta-propeller domain
WD40	PF00400	289 - 324	2.6e-07	WD domain, G-beta repeat
WD40_Prp19	PF24814	306 - 392	1.2e-08	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	353 - 423	2e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24530
fragaria_vesca	FvH4_4g25271
malus_domestica	MD13G1105300.v1.1 MD16G1105400.v1.1
prunus_persica	Prupe.1G241300_v2.0.a1
pyrus_communis	pycom13g09130 pycom16g08970
rosa_chinensis	RchiOBHm_Chr4g0432531
rosa_laevigata	RLG00000006834
rosa_multiflora	Rmu_sc0009980.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373980
rosa_rugosa	Rorug04G0260000.1
rosa_samantha	Rh4AG314800 Rh4BG322500 Rh4CG337700 Rh4DG318500
rosa_wichuraiana	Rw4G027370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	79, 254
AatII	GACGTC	1 cut(s)	77
AccB7I	CCANNNNNTGG	1 cut(s)	865
AccI	GTMKAC	1 cut(s)	369
AccII	CGCG	1 cut(s)	1110
AclWI	GGATC	9 cut(s)	6, 423, 436, 619, 632, 729, 817, 830, 934
AcoI	YGGCCR	1 cut(s)	909
AcuI	CTGAAG	1 cut(s)	722
AcvI	CACGTG	2 cut(s)	862, 1156
AcyI	GRCGYC	4 cut(s)	74, 639, 759, 1206
AfaI	GTAC	4 cut(s)	142, 379, 800, 1282
AfiI	CCNNNNNNNGG	3 cut(s)	865, 970, 1000
AflIII	ACRYGT	1 cut(s)	861
AgsI	TTSAA	4 cut(s)	406, 535, 960, 1306
AhlI	ACTAGT	1 cut(s)	137
AjiI	CACGTC	1 cut(s)	1258
AjnI	CCWGG	4 cut(s)	14, 477, 686, 963
AloI	GAACNNNNNNTCC	2 cut(s)	1052, 1084
AluBI	AGCT	3 cut(s)	117, 529, 1230
AluI	AGCT	3 cut(s)	117, 529, 1230
Alw26I	GTCTC	5 cut(s)	65, 87, 262, 1264, 1302
AlwI	GGATC	9 cut(s)	6, 423, 436, 619, 632, 729, 817, 830, 934
AlwNI	CAGNNNCTG	1 cut(s)	199
Ama87I	CYCGRG	2 cut(s)	84, 424
AoxI	GGCC	4 cut(s)	352, 667, 909, 1288
ApaI	GGGCCC	1 cut(s)	1292
ApeKI	GCWGC	4 cut(s)	114, 168, 199, 729
ArsI	GACNNNNNNTTYG	2 cut(s)	480, 512
AspLEI	GCGC	6 cut(s)	149, 567, 751, 858, 989, 1015
AspS9I	GGNCC	5 cut(s)	352, 382, 834, 1288, 1289
AsuC2I	CCSGG	3 cut(s)	805, 827, 1287
AsuHPI	GGTGA	6 cut(s)	69, 637, 1015, 1016, 1105, 1177
AsuII	TTCGAA	1 cut(s)	1268
AsuNHI	GCTAGC	1 cut(s)	19
AvaI	CYCGRG	2 cut(s)	84, 424
AvaII	GGWCC	2 cut(s)	382, 834
BaeGI	GKGCMC	2 cut(s)	1155, 1292
BalI	TGGCCA	1 cut(s)	911
BamHI	GGATCC	3 cut(s)	428, 624, 822
BanII	GRGCYC	1 cut(s)	1292
BauI	CACGAG	2 cut(s)	356, 752
BbrPI	CACGTG	2 cut(s)	862, 1156
BbvI	GCAGC	4 cut(s)	126, 180, 186, 716
BccI	CCATC	3 cut(s)	32, 211, 1229
BceAI	ACGGC	9 cut(s)	175, 276, 305, 525, 626, 641, 682, 746, 1219
BciT130I	CCWGG	4 cut(s)	16, 479, 688, 965
BciVI	GTATCC	1 cut(s)	694
BclI	TGATCA	1 cut(s)	1047
BcnI	CCSGG	3 cut(s)	805, 827, 1287
BcoDI	GTCTC	5 cut(s)	65, 87, 262, 1264, 1302
BcuI	ACTAGT	1 cut(s)	137
BfaI	CTAG	3 cut(s)	20, 138, 236
BfoI	RGCGCY	3 cut(s)	859, 990, 1016
BfuI	GTATCC	1 cut(s)	694
BisI	GCNGC	8 cut(s)	115, 169, 200, 345, 568, 604, 730, 1111
BlsI	GCNGC	8 cut(s)	116, 170, 201, 346, 569, 605, 731, 1112
Bme1390I	CCNGG	7 cut(s)	16, 479, 688, 805, 827, 965, 1287
Bme18I	GGWCC	2 cut(s)	382, 834
BmeT110I	CYCGRG	2 cut(s)	84, 424
BmgBI	CACGTC	1 cut(s)	1258
BmgT120I	GGNCC	5 cut(s)	352, 382, 834, 1288, 1289
BmiI	GGNNCC	5 cut(s)	8, 430, 626, 824, 1290
BmrFI	CCNGG	7 cut(s)	16, 479, 688, 805, 827, 965, 1287
BmsI	GCATC	3 cut(s)	356, 406, 660
BmtI	GCTAGC	1 cut(s)	23
BpmI	CTGGAG	3 cut(s)	176, 203, 899
Bpu14I	TTCGAA	1 cut(s)	1268
BpuMI	CCSGG	3 cut(s)	805, 827, 1287
BsaAI	YACGTR	3 cut(s)	592, 862, 1156
BsaHI	GRCGYC	4 cut(s)	74, 639, 759, 1206
BsaJI	CCNNGG	2 cut(s)	423, 687
BsaWI	WCCGGW	1 cut(s)	1026
BsaXI	ACNNNNNCTCC	6 cut(s)	404, 434, 467, 497, 1114, 1144
Bsc4I	CCNNNNNNNGG	3 cut(s)	865, 970, 1000
Bse118I	RCCGGY	2 cut(s)	647, 1162
BseBI	CCWGG	4 cut(s)	16, 479, 688, 965
BseDI	CCNNGG	2 cut(s)	423, 687
BseGI	GGATG	1 cut(s)	1240
BseLI	CCNNNNNNNGG	3 cut(s)	865, 970, 1000
BseMII	CTCAG	4 cut(s)	60, 693, 1007, 1313
BseRI	GAGGAG	5 cut(s)	19, 109, 326, 412, 1205
BseSI	GKGCMC	2 cut(s)	1155, 1292
BseXI	GCAGC	4 cut(s)	126, 180, 186, 716
BseYI	CCCAGC	1 cut(s)	131
Bsh1236I	CGCG	1 cut(s)	1110
Bsh1285I	CGRYCG	3 cut(s)	376, 559, 955
BshFI	GGCC	4 cut(s)	354, 669, 911, 1290
BsiEI	CGRYCG	3 cut(s)	376, 559, 955
BsiHKCI	CYCGRG	2 cut(s)	84, 424
BsiSI	CCGG	6 cut(s)	648, 804, 826, 1027, 1163, 1286
BsiWI	CGTACG	1 cut(s)	377
BslFI	GGGAC	5 cut(s)	38, 279, 704, 728, 805
BslI	CCNNNNNNNGG	3 cut(s)	865, 970, 1000
BsmAI	GTCTC	5 cut(s)	65, 87, 262, 1264, 1302
BsmBI	CGTCTC	4 cut(s)	65, 87, 262, 1264
BsmFI	GGGAC	5 cut(s)	38, 279, 704, 728, 805
BsnI	GGCC	4 cut(s)	354, 669, 911, 1290
BsoBI	CYCGRG	2 cut(s)	84, 424
Bsp119I	TTCGAA	1 cut(s)	1268
Bsp120I	GGGCCC	1 cut(s)	1288
Bsp1286I	GDGCHC	2 cut(s)	1155, 1292
Bsp1407I	TGTACA	1 cut(s)	798
BspANI	GGCC	4 cut(s)	354, 669, 911, 1290
BspCNI	CTCAG	4 cut(s)	61, 692, 1008, 1312
BspFNI	CGCG	1 cut(s)	1110
BspLI	GGNNCC	5 cut(s)	8, 430, 626, 824, 1290
BspOI	GCTAGC	1 cut(s)	23
BspPI	GGATC	9 cut(s)	6, 423, 436, 619, 632, 729, 817, 830, 934
BspT104I	TTCGAA	1 cut(s)	1268
BsrFI	RCCGGY	2 cut(s)	647, 1162
BsrGI	TGTACA	1 cut(s)	798
BssAI	RCCGGY	2 cut(s)	647, 1162
BssECI	CCNNGG	2 cut(s)	423, 687
BssNI	GRCGYC	4 cut(s)	74, 639, 759, 1206
BssSI	CACGAG	2 cut(s)	356, 752
Bst2BI	CACGAG	2 cut(s)	356, 752
Bst2UI	CCWGG	4 cut(s)	16, 479, 688, 965
BstACI	GRCGYC	4 cut(s)	74, 639, 759, 1206
BstAUI	TGTACA	1 cut(s)	798
BstBAI	YACGTR	3 cut(s)	592, 862, 1156
BstBI	TTCGAA	1 cut(s)	1268
BstC8I	GCNNGC	3 cut(s)	21, 151, 949
BstDEI	CTNAG	4 cut(s)	69, 679, 1016, 1299
BstEII	GGTNACC	1 cut(s)	1022
BstF5I	GGATG	1 cut(s)	1240
BstFNI	CGCG	1 cut(s)	1110
BstH2I	RGCGCY	3 cut(s)	859, 990, 1016
BstHHI	GCGC	6 cut(s)	149, 567, 751, 858, 989, 1015
BstMAI	GTCTC	5 cut(s)	65, 87, 262, 1264, 1302
BstMCI	CGRYCG	3 cut(s)	376, 559, 955
BstMWI	GCNNNNNNNGC	8 cut(s)	159, 168, 177, 416, 767, 883, 902, 1159
BstNI	CCWGG	4 cut(s)	16, 479, 688, 965
BstNSI	RCATGY	1 cut(s)	315
BstPI	GGTNACC	1 cut(s)	1022
BstSCI	CCNGG	7 cut(s)	14, 477, 686, 803, 825, 963, 1285
BstSLI	GKGCMC	2 cut(s)	1155, 1292
BstUI	CGCG	1 cut(s)	1110
BstV1I	GCAGC	4 cut(s)	126, 180, 186, 716
BstX2I	RGATCY	4 cut(s)	428, 624, 721, 822
BstXI	CCANNNNNNTGG	1 cut(s)	997
BstYI	RGATCY	4 cut(s)	428, 624, 721, 822
BsuI	GTATCC	1 cut(s)	694
BsuRI	GGCC	4 cut(s)	354, 669, 911, 1290
BtgZI	GCGATG	1 cut(s)	637
BtrI	CACGTC	1 cut(s)	1258
BtsCI	GGATG	1 cut(s)	1240
BtsI	GCAGTG	2 cut(s)	777, 903
BtsIMutI	CAGTG	5 cut(s)	777, 903, 1078, 1228, 1292
Cac8I	GCNNGC	3 cut(s)	21, 151, 949
CaiI	CAGNNNCTG	1 cut(s)	199
CfoI	GCGC	6 cut(s)	149, 567, 751, 858, 989, 1015
Cfr10I	RCCGGY	2 cut(s)	647, 1162
Cfr13I	GGNCC	5 cut(s)	352, 382, 834, 1288, 1289
CseI	GACGC	6 cut(s)	205, 647, 721, 767, 883, 1195
Csp6I	GTAC	4 cut(s)	141, 378, 799, 1281
CviAII	CATG	1 cut(s)	312
CviQI	GTAC	4 cut(s)	141, 378, 799, 1281
DdeI	CTNAG	4 cut(s)	69, 679, 1016, 1299
DrdI	GACNNNNNNGTC	2 cut(s)	79, 254
DseDI	GACNNNNNNGTC	2 cut(s)	79, 254
EaeI	YGGCCR	1 cut(s)	909
EciI	GGCGGA	2 cut(s)	590, 827
Eco24I	GRGCYC	1 cut(s)	1292
Eco47I	GGWCC	2 cut(s)	382, 834
Eco57I	CTGAAG	1 cut(s)	722
Eco72I	CACGTG	2 cut(s)	862, 1156
Eco88I	CYCGRG	2 cut(s)	84, 424
Eco91I	GGTNACC	1 cut(s)	1022
EcoO65I	GGTNACC	1 cut(s)	1022
EcoRII	CCWGG	4 cut(s)	14, 477, 686, 963
EcoT38I	GRGCYC	1 cut(s)	1292
Esp3I	CGTCTC	4 cut(s)	65, 87, 262, 1264
FaeI	CATG	1 cut(s)	315
FaiI	YATR	6 cut(s)	313, 914, 996, 998, 1037, 1046
FaqI	GGGAC	5 cut(s)	38, 279, 704, 728, 805
FatI	CATG	1 cut(s)	311
FauI	CCCGC	5 cut(s)	47, 291, 296, 505, 705
FauNDI	CATATG	1 cut(s)	996
FbaI	TGATCA	1 cut(s)	1047
FblI	GTMKAC	1 cut(s)	369
Fnu4HI	GCNGC	8 cut(s)	115, 169, 200, 345, 568, 604, 730, 1111
FokI	GGATG	1 cut(s)	1247
FriOI	GRGCYC	1 cut(s)	1292
Fsp4HI	GCNGC	8 cut(s)	115, 169, 200, 345, 568, 604, 730, 1111
FspBI	CTAG	3 cut(s)	20, 138, 236
GlaI	GCGC	6 cut(s)	148, 566, 750, 857, 988, 1014
GluI	GCNGC	8 cut(s)	115, 169, 200, 345, 568, 604, 730, 1111
GsaI	CCCAGC	1 cut(s)	135
GsuI	CTGGAG	3 cut(s)	176, 203, 899
HaeII	RGCGCY	3 cut(s)	859, 990, 1016
HaeIII	GGCC	4 cut(s)	354, 669, 911, 1290
HapII	CCGG	6 cut(s)	648, 804, 826, 1027, 1163, 1286
HgaI	GACGC	6 cut(s)	205, 647, 721, 767, 883, 1195
HhaI	GCGC	6 cut(s)	149, 567, 751, 858, 989, 1015
Hin1I	GRCGYC	4 cut(s)	74, 639, 759, 1206
Hin1II	CATG	1 cut(s)	315
Hin6I	GCGC	6 cut(s)	147, 565, 749, 856, 987, 1013
HinP1I	GCGC	6 cut(s)	147, 565, 749, 856, 987, 1013
HincII	GTYRAC	3 cut(s)	507, 553, 661
HindII	GTYRAC	3 cut(s)	507, 553, 661
HindIII	AAGCTT	1 cut(s)	527
HinfI	GANTC	5 cut(s)	398, 482, 495, 716, 737
HpaII	CCGG	6 cut(s)	648, 804, 826, 1027, 1163, 1286
HphI	GGTGA	6 cut(s)	69, 637, 1015, 1016, 1105, 1177
Hpy188I	TCNGA	8 cut(s)	11, 106, 124, 454, 494, 721, 822, 1089
Hpy188III	TCNNGA	6 cut(s)	193, 220, 236, 395, 426, 622
Hpy99I	CGWCG	8 cut(s)	458, 737, 791, 877, 923, 926, 1058, 1211
HpyAV	CCTTC	2 cut(s)	1024, 1240
HpyCH4IV	ACGT	7 cut(s)	74, 366, 591, 861, 1053, 1155, 1257
HpyF10VI	GCNNNNNNNGC	8 cut(s)	159, 168, 177, 416, 767, 883, 902, 1159
HpyF3I	CTNAG	4 cut(s)	69, 679, 1016, 1299
HpySE526I	ACGT	7 cut(s)	74, 366, 591, 861, 1053, 1155, 1257
Hsp92I	GRCGYC	4 cut(s)	74, 639, 759, 1206
Hsp92II	CATG	1 cut(s)	315
HspAI	GCGC	6 cut(s)	147, 565, 749, 856, 987, 1013
Ksp22I	TGATCA	1 cut(s)	1047
LmnI	GCTCC	3 cut(s)	122, 1192, 1227
Lsp1109I	GCAGC	4 cut(s)	126, 180, 186, 716
LweI	GCATC	3 cut(s)	356, 406, 660
MaeI	CTAG	3 cut(s)	20, 138, 236
MaeII	ACGT	7 cut(s)	74, 366, 591, 861, 1053, 1155, 1257
MaeIII	GTNAC	6 cut(s)	75, 592, 643, 1003, 1022, 1210
MboII	GAAGA	2 cut(s)	457, 715
MflI	RGATCY	4 cut(s)	428, 624, 721, 822
MhlI	GDGCHC	2 cut(s)	1155, 1292
MlsI	TGGCCA	1 cut(s)	911
MluCI	AATT	1 cut(s)	445
MluNI	TGGCCA	1 cut(s)	911
MlyI	GAGTC	4 cut(s)	476, 504, 710, 746
MmeI	TCCRAC	4 cut(s)	87, 477, 652, 800
Mox20I	TGGCCA	1 cut(s)	911
MscI	TGGCCA	1 cut(s)	911
MseI	TTAA	3 cut(s)	435, 444, 1185
Msp20I	TGGCCA	1 cut(s)	911
MspA1I	CMGCKG	1 cut(s)	729
MspI	CCGG	6 cut(s)	648, 804, 826, 1027, 1163, 1286
MspR9I	CCNGG	7 cut(s)	16, 479, 688, 805, 827, 965, 1287
MvaI	CCWGG	4 cut(s)	16, 479, 688, 965
MvnI	CGCG	1 cut(s)	1110
MwoI	GCNNNNNNNGC	8 cut(s)	159, 168, 177, 416, 767, 883, 902, 1159
NciI	CCSGG	3 cut(s)	805, 827, 1287
NdeI	CATATG	1 cut(s)	996
NheI	GCTAGC	1 cut(s)	19
NlaIII	CATG	1 cut(s)	315
NlaIV	GGNNCC	5 cut(s)	8, 430, 626, 824, 1290
NmuCI	GTSAC	5 cut(s)	75, 592, 643, 1003, 1022
NspI	RCATGY	1 cut(s)	315
NspV	TTCGAA	1 cut(s)	1268
PcsI	WCGNNNNNNNCGW	4 cut(s)	252, 363, 639, 759
PfeI	GAWTC	1 cut(s)	398
Pfl23II	CGTACG	1 cut(s)	377
PflFI	GACNNNGTC	1 cut(s)	641
PflMI	CCANNNNNTGG	1 cut(s)	865
PkrI	GCNGC	8 cut(s)	116, 170, 201, 346, 569, 605, 731, 1112
Ple19I	CGATCG	1 cut(s)	376
PleI	GAGTC	4 cut(s)	476, 503, 710, 745
PmaCI	CACGTG	2 cut(s)	862, 1156
PmlI	CACGTG	2 cut(s)	862, 1156
PpsI	GAGTC	4 cut(s)	476, 503, 710, 745
Ppu21I	YACGTR	3 cut(s)	592, 862, 1156
Psp6I	CCWGG	4 cut(s)	14, 477, 686, 963
PspCI	CACGTG	2 cut(s)	862, 1156
PspEI	GGTNACC	1 cut(s)	1022
PspFI	CCCAGC	1 cut(s)	131
PspGI	CCWGG	4 cut(s)	14, 477, 686, 963
PspLI	CGTACG	1 cut(s)	377
PspN4I	GGNNCC	5 cut(s)	8, 430, 626, 824, 1290
PspOMI	GGGCCC	1 cut(s)	1288
PspPI	GGNCC	5 cut(s)	352, 382, 834, 1288, 1289
PstNI	CAGNNNCTG	1 cut(s)	199
PsuI	RGATCY	4 cut(s)	428, 624, 721, 822
PsyI	GACNNNGTC	1 cut(s)	641
PvuI	CGATCG	1 cut(s)	376
RsaI	GTAC	4 cut(s)	142, 379, 800, 1282
RsaNI	GTAC	4 cut(s)	141, 378, 799, 1281
SaqAI	TTAA	3 cut(s)	435, 444, 1185
SatI	GCNGC	8 cut(s)	115, 169, 200, 345, 568, 604, 730, 1111
Sau96I	GGNCC	5 cut(s)	352, 382, 834, 1288, 1289
SchI	GAGTC	4 cut(s)	476, 504, 710, 746
ScrFI	CCNGG	7 cut(s)	16, 479, 688, 805, 827, 965, 1287
SduI	GDGCHC	2 cut(s)	1155, 1292
SfaNI	GCATC	3 cut(s)	356, 406, 660
SfuI	TTCGAA	1 cut(s)	1268
SinI	GGWCC	2 cut(s)	382, 834
SpeI	ACTAGT	1 cut(s)	137
Sse9I	AATT	1 cut(s)	445
SspMI	CTAG	3 cut(s)	20, 138, 236
StyD4I	CCNGG	7 cut(s)	14, 477, 686, 803, 825, 963, 1285
TaiI	ACGT	7 cut(s)	77, 369, 594, 864, 1056, 1158, 1260
TaqI	TCGA	6 cut(s)	246, 396, 735, 782, 1199, 1268
TasI	AATT	1 cut(s)	445
TatI	WGTACW	1 cut(s)	798
TauI	GCSGC	4 cut(s)	347, 570, 606, 1113
TfiI	GAWTC	1 cut(s)	398
Tru1I	TTAA	3 cut(s)	435, 444, 1185
Tru9I	TTAA	3 cut(s)	435, 444, 1185
TscAI	CASTG	5 cut(s)	777, 903, 1078, 1228, 1299
TseFI	GTSAC	5 cut(s)	75, 592, 643, 1003, 1022
TseI	GCWGC	4 cut(s)	114, 168, 199, 729
Tsp45I	GTSAC	5 cut(s)	75, 592, 643, 1003, 1022
TspDTI	ATGAA	1 cut(s)	390
TspGWI	ACGGA	5 cut(s)	244, 395, 421, 430, 939
TspRI	CASTG	5 cut(s)	777, 903, 1078, 1228, 1299
Tth111I	GACNNNGTC	1 cut(s)	641
Van91I	CCANNNNNTGG	1 cut(s)	865
VpaK11BI	GGWCC	2 cut(s)	382, 834
XbaI	TCTAGA	1 cut(s)	235
XceI	RCATGY	1 cut(s)	315
XmiI	GTMKAC	1 cut(s)	369
XspI	CTAG	3 cut(s)	20, 138, 236
ZraI	GACGTC	1 cut(s)	75

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.