Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014209

Physical Location & Seq

Genomic Coordinates Reverse (-)

7381199 .. 7382512

Gene Structure 1314 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1105400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGAGGGTCCGATCCTGGCTAGCCATCTGCTCCGCCAACAGCAAATCCACCATCCCATTCTCCAAACCTGAGACGTCACCATCTCCGACGAAACTCGAAACTTCCGATACGAGCAGCACCTCTGATGTTCCCAGCACTAGCACTAGCGCTAGCGACACCAGCCGTAGCAGCCTCCAAAGCAACCTCTCCCTCCAGACGCTGCCATCTGTCGTCTCTCTCCAGAAACTCTCCCCTCTAGACGCCCTCGACCTCTCCGTCTCCCACCTCTGCTTCGCCACTCTCAGGCCCCGCCGCCCCTCCCTCCCCATCACCTGCCTCGCCATCCACCACAACCTCCTCTACGCTGCATCCGGCCACGAGATCAACGTCTATGATCGGACGACCCACGCGCTTCACGATTCATTCAACGCCGACGATGCCTCCTCGGGCTCCGTTAAGTCCGTTAATTTTTCCAACAGCAACATATTCACTTCACACCAGGACTCCAAAATACGAGTCTGGCAGTTGACGGTGGGCAAACGGCACAAGCTATTAAACTCCCTCCCCACCGTCAATGATCGTTTGCGCCGTTTCGTACTCCCCAAGAACTACGTCACCGTCCGCCGCCACAAGAAATGCCTCTGGATCCAGCACGCCGACGCCGTCACCGGCATCGCCGTCAACAATGGCCTGATTTACTCCGTCTCCTGGGACAAGAGCCTGAAGATTTGGCGGGACTCGGATCTCCGCTGCGTCGAGTCCGTCAAGGCACACGAGGACGCCGTGAACGCGGTGGCGGTATCGAACGACGGGACGGTATACACCGGATCGGCGGATTGTCGGATCCGGGTATGGGCCAAACCGTTCGGGGAGAAGCGCCACGTGTTGGTGGCGACTCTGGAGAAGCACAAGTCAGCGGTGAACGCTTTGGCCCTAAACGACGACGGATCGGTGCTGTTTTCAGGGGCTTGCGACCGTTCGATCCTGGTGTGGGAGAGGGAGGACAGCGCGAACCACATGGCGGTGACGGGGGCTCTGAGAGGTCACCGGAAGGCTATACTGTGTATGATCAACGTCGGGGATTTGTTATTCAGTGGATCGGCTGATCGGACAGTGAGGGTGTGGCAGCGTGGCGAGGACGGGAGTTTTTGTTGTTTGGCGGTTTTGGAGGGGCACGTGAAGCCGGTGAAGTCGTTGGTGGCGGTTAAAGACGAAGAGTCAAGCGGCGTCGTTACGGTTTATAGTGGGAGCTTGGATGGAGAAGTGAAGGTGTGGCATGTCTCGGTTTCGAACGTGAACAGTTCGCCGGGCTTACCGTCTGAGTTGCAAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007049 GO:0008092 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016905 GO:0017022 GO:0017076 GO:0018107 GO:0018193 GO:0018210 GO:0019538 GO:0022402 GO:0022411 GO:0030029 GO:0030036 GO:0030554 GO:0030863 GO:0030864 GO:0031033 GO:0031035 GO:0031037 GO:0031038 GO:0032153 GO:0032155 GO:0032553 GO:0032555 GO:0032559 GO:0032984 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045159 GO:0046777 GO:0051301 GO:0061640 GO:0070938 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0099568 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

438

Amino Acids

47.7

Weight (kDa)

8.85

Isoelectric Point (pI)

45.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_2nd	PF25172	99 - 372	3.2e-19	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	100 - 179	6.1e-06	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	103 - 327	5.7e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_SCAP	PF24017	108 - 332	7.9e-06	SCAP Beta-propeller
Beta-prop_WDR5	PF25175	210 - 289	5e-10	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	210 - 279	6.6e-08	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	211 - 369	3.9e-12	WDHD1 first WD40 domain
NBCH_WD40	PF20426	218 - 279	1.3e-07	Neurobeachin beta propeller domain
WD40_Prp19	PF24814	220 - 322	1.2e-11	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	223 - 349	5.9e-09	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	223 - 421	2.4e-10	WDR55
WD40_CDC20-Fz	PF24807	231 - 369	3.4e-13	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	231 - 421	4.3e-16	TEP-1 second beta-propeller
WD40	PF00400	243 - 278	6.3e-07	WD domain, G-beta repeat
Beta-prop_WDR75_1st	PF23869	253 - 330	3.4e-07	WD repeat-containing protein 75 first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	261 - 386	6.4e-08	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	261 - 346	2e-07	Prp19 WD40 domain
WD40_Gbeta	PF25391	265 - 377	7.2e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	270 - 424	1.6e-20	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	289 - 424	4.2e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	289 - 420	1.3e-17	WDR3 first beta-propeller domain
WD40	PF00400	289 - 324	3.9e-07	WD domain, G-beta repeat
EIF3I	PF24805	293 - 380	4.7e-06	EIF3I
WD40_Prp19	PF24814	308 - 392	1.9e-08	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	353 - 423	3.1e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24530
fragaria_vesca	FvH4_4g25271
malus_domestica	MD13G1105300.v1.1 MD16G1105400.v1.1
prunus_persica	Prupe.1G241300_v2.0.a1
pyrus_communis	pycom13g09130 pycom16g08970
rosa_chinensis	RchiOBHm_Chr4g0432531
rosa_laevigata	RLG00000006834
rosa_multiflora	Rmu_sc0009980.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373980
rosa_rugosa	Rorug04G0260000.1
rosa_samantha	Rh4AG314800 Rh4BG322500 Rh4CG337700 Rh4DG318500
rosa_wichuraiana	Rw4G027370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	320
AasI	GACNNNNNNGTC	1 cut(s)	254
AatII	GACGTC	1 cut(s)	77
Acc36I	ACCTGC	1 cut(s)	320
AccB7I	CCANNNNNTGG	1 cut(s)	865
AccI	GTMKAC	1 cut(s)	798
AccII	CGCG	3 cut(s)	389, 770, 989
AcoI	YGGCCR	1 cut(s)	352
AcuI	CTGAAG	1 cut(s)	722
AcvI	CACGTG	2 cut(s)	862, 1156
AcyI	GRCGYC	5 cut(s)	74, 240, 639, 759, 1206
AfaI	GTAC	1 cut(s)	576
AfeI	AGCGCT	1 cut(s)	148
AfiI	CCNNNNNNNGG	6 cut(s)	282, 687, 832, 865, 970, 1000
AflIII	ACRYGT	1 cut(s)	861
AgsI	TTSAA	1 cut(s)	406
AhdI	GACNNNNNGTC	1 cut(s)	1195
AjnI	CCWGG	4 cut(s)	14, 477, 686, 963
AluBI	AGCT	2 cut(s)	529, 1230
AluI	AGCT	2 cut(s)	529, 1230
Alw26I	GTCTC	5 cut(s)	65, 217, 262, 688, 1264
AlwNI	CAGNNNCTG	1 cut(s)	199
Ama87I	CYCGRG	1 cut(s)	424
Aor51HI	AGCGCT	1 cut(s)	148
AoxI	GGCC	5 cut(s)	284, 352, 667, 834, 909
ApeKI	GCWGC	6 cut(s)	114, 168, 199, 344, 729, 1105
ArsI	GACNNNNNNTTYG	2 cut(s)	480, 512
AspLEI	GCGC	5 cut(s)	149, 391, 567, 858, 989
AspS9I	GGNCC	4 cut(s)	7, 285, 834, 910
AsuC2I	CCSGG	2 cut(s)	827, 1287
AsuHPI	GGTGA	8 cut(s)	69, 301, 586, 637, 910, 1015, 1016, 1177
AsuII	TTCGAA	1 cut(s)	1268
AsuNHI	GCTAGC	2 cut(s)	19, 149
AvaI	CYCGRG	1 cut(s)	424
AvaII	GGWCC	1 cut(s)	7
BaeGI	GKGCMC	1 cut(s)	1155
BamHI	GGATCC	2 cut(s)	624, 822
BanII	GRGCYC	2 cut(s)	431, 1015
BauI	CACGAG	2 cut(s)	356, 752
BbrPI	CACGTG	2 cut(s)	862, 1156
BbvI	GCAGC	6 cut(s)	126, 180, 186, 331, 716, 1117
BccI	CCATC	7 cut(s)	32, 59, 88, 211, 314, 329, 1229
BceAI	ACGGC	6 cut(s)	147, 536, 552, 626, 641, 746
BciT130I	CCWGG	4 cut(s)	16, 479, 688, 965
BclI	TGATCA	1 cut(s)	1047
BcnI	CCSGG	2 cut(s)	827, 1287
BcoDI	GTCTC	5 cut(s)	65, 217, 262, 688, 1264
BfaI	CTAG	5 cut(s)	20, 138, 144, 150, 236
BfoI	RGCGCY	2 cut(s)	150, 859
BfuAI	ACCTGC	1 cut(s)	320
BisI	GCNGC	9 cut(s)	115, 169, 200, 292, 345, 604, 730, 1106, 1204
BlsI	GCNGC	9 cut(s)	116, 170, 201, 293, 346, 605, 731, 1107, 1205
Bme1390I	CCNGG	6 cut(s)	16, 479, 688, 827, 965, 1287
Bme18I	GGWCC	1 cut(s)	7
BmeRI	GACNNNNNGTC	1 cut(s)	1195
BmeT110I	CYCGRG	1 cut(s)	424
BmgT120I	GGNCC	4 cut(s)	7, 285, 834, 910
BmiI	GGNNCC	5 cut(s)	8, 287, 430, 626, 824
BmrFI	CCNGG	6 cut(s)	16, 479, 688, 827, 965, 1287
BmsI	GCATC	3 cut(s)	356, 406, 660
BmtI	GCTAGC	2 cut(s)	23, 153
BpmI	CTGGAG	3 cut(s)	176, 203, 899
Bpu14I	TTCGAA	1 cut(s)	1268
BpuMI	CCSGG	2 cut(s)	827, 1287
BsaAI	YACGTR	2 cut(s)	862, 1156
BsaHI	GRCGYC	5 cut(s)	74, 240, 639, 759, 1206
BsaJI	CCNNGG	2 cut(s)	423, 687
BsaWI	WCCGGW	2 cut(s)	803, 1026
BsaXI	ACNNNNNCTCC	8 cut(s)	404, 434, 467, 497, 668, 698, 1114, 1144
Bsc4I	CCNNNNNNNGG	6 cut(s)	282, 687, 832, 865, 970, 1000
Bse118I	RCCGGY	2 cut(s)	647, 1162
BseBI	CCWGG	4 cut(s)	16, 479, 688, 965
BseDI	CCNNGG	2 cut(s)	423, 687
BseGI	GGATG	4 cut(s)	51, 321, 347, 1240
BseLI	CCNNNNNNNGG	6 cut(s)	282, 687, 832, 865, 970, 1000
BseMII	CTCAG	4 cut(s)	60, 295, 1007, 1290
BseRI	GAGGAG	2 cut(s)	326, 412
BseSI	GKGCMC	1 cut(s)	1155
BseXI	GCAGC	6 cut(s)	126, 180, 186, 331, 716, 1117
BseYI	CCCAGC	1 cut(s)	131
Bsh1236I	CGCG	3 cut(s)	389, 770, 989
Bsh1285I	CGRYCG	1 cut(s)	955
BshFI	GGCC	5 cut(s)	286, 354, 669, 836, 911
BsiEI	CGRYCG	1 cut(s)	955
BsiHKCI	CYCGRG	1 cut(s)	424
BsiSI	CCGG	7 cut(s)	351, 648, 804, 826, 1027, 1163, 1286
BslFI	GGGAC	3 cut(s)	704, 728, 805
BslI	CCNNNNNNNGG	6 cut(s)	282, 687, 832, 865, 970, 1000
BsmAI	GTCTC	5 cut(s)	65, 217, 262, 688, 1264
BsmBI	CGTCTC	4 cut(s)	65, 217, 262, 688
BsmFI	GGGAC	3 cut(s)	704, 728, 805
BsnI	GGCC	5 cut(s)	286, 354, 669, 836, 911
BsoBI	CYCGRG	1 cut(s)	424
Bsp119I	TTCGAA	1 cut(s)	1268
Bsp1286I	GDGCHC	3 cut(s)	431, 1015, 1155
BspANI	GGCC	5 cut(s)	286, 354, 669, 836, 911
BspCNI	CTCAG	4 cut(s)	61, 294, 1008, 1291
BspFNI	CGCG	3 cut(s)	389, 770, 989
BspLI	GGNNCC	5 cut(s)	8, 287, 430, 626, 824
BspMI	ACCTGC	1 cut(s)	320
BspOI	GCTAGC	2 cut(s)	23, 153
BspT104I	TTCGAA	1 cut(s)	1268
BsrFI	RCCGGY	2 cut(s)	647, 1162
BssAI	RCCGGY	2 cut(s)	647, 1162
BssECI	CCNNGG	2 cut(s)	423, 687
BssNAI	GTATAC	1 cut(s)	799
BssNI	GRCGYC	5 cut(s)	74, 240, 639, 759, 1206
BssSI	CACGAG	2 cut(s)	356, 752
Bst1107I	GTATAC	1 cut(s)	799
Bst2BI	CACGAG	2 cut(s)	356, 752
Bst2UI	CCWGG	4 cut(s)	16, 479, 688, 965
Bst6I	CTCTTC	1 cut(s)	1188
BstACI	GRCGYC	5 cut(s)	74, 240, 639, 759, 1206
BstBAI	YACGTR	2 cut(s)	862, 1156
BstBI	TTCGAA	1 cut(s)	1268
BstC8I	GCNNGC	4 cut(s)	21, 151, 633, 949
BstDEI	CTNAG	4 cut(s)	69, 281, 1016, 1299
BstEII	GGTNACC	1 cut(s)	1022
BstF5I	GGATG	4 cut(s)	51, 321, 347, 1240
BstFNI	CGCG	3 cut(s)	389, 770, 989
BstH2I	RGCGCY	2 cut(s)	150, 859
BstHHI	GCGC	5 cut(s)	149, 391, 567, 858, 989
BstMAI	GTCTC	5 cut(s)	65, 217, 262, 688, 1264
BstMCI	CGRYCG	1 cut(s)	955
BstMWI	GCNNNNNNNGC	7 cut(s)	159, 168, 177, 416, 767, 902, 1159
BstNI	CCWGG	4 cut(s)	16, 479, 688, 965
BstNSI	RCATGY	1 cut(s)	1259
BstPI	GGTNACC	1 cut(s)	1022
BstSCI	CCNGG	6 cut(s)	14, 477, 686, 825, 963, 1285
BstSLI	GKGCMC	1 cut(s)	1155
BstUI	CGCG	3 cut(s)	389, 770, 989
BstV1I	GCAGC	6 cut(s)	126, 180, 186, 331, 716, 1117
BstX2I	RGATCY	3 cut(s)	624, 721, 822
BstYI	RGATCY	3 cut(s)	624, 721, 822
BstZ17I	GTATAC	1 cut(s)	799
BsuRI	GGCC	5 cut(s)	286, 354, 669, 836, 911
BtgZI	GCGATG	1 cut(s)	637
BtsCI	GGATG	4 cut(s)	51, 321, 347, 1240
BtsIMutI	CAGTG	2 cut(s)	1078, 1098
BveI	ACCTGC	1 cut(s)	320
Cac8I	GCNNGC	4 cut(s)	21, 151, 633, 949
CaiI	CAGNNNCTG	1 cut(s)	199
CfoI	GCGC	5 cut(s)	149, 391, 567, 858, 989
Cfr10I	RCCGGY	2 cut(s)	647, 1162
Cfr13I	GGNCC	4 cut(s)	7, 285, 834, 910
CseI	GACGC	6 cut(s)	205, 248, 647, 721, 767, 1195
Csp6I	GTAC	1 cut(s)	575
CviAII	CATG	2 cut(s)	997, 1256
CviQI	GTAC	1 cut(s)	575
DdeI	CTNAG	4 cut(s)	69, 281, 1016, 1299
DrdI	GACNNNNNNGTC	1 cut(s)	254
DriI	GACNNNNNGTC	1 cut(s)	1195
DseDI	GACNNNNNNGTC	1 cut(s)	254
EaeI	YGGCCR	1 cut(s)	352
Eam1104I	CTCTTC	1 cut(s)	1188
Eam1105I	GACNNNNNGTC	1 cut(s)	1195
EarI	CTCTTC	1 cut(s)	1188
EciI	GGCGGA	3 cut(s)	22, 590, 827
Eco24I	GRGCYC	2 cut(s)	431, 1015
Eco47I	GGWCC	1 cut(s)	7
Eco47III	AGCGCT	1 cut(s)	148
Eco57I	CTGAAG	1 cut(s)	722
Eco72I	CACGTG	2 cut(s)	862, 1156
Eco88I	CYCGRG	1 cut(s)	424
Eco91I	GGTNACC	1 cut(s)	1022
EcoO109I	RGGNCCY	1 cut(s)	285
EcoO65I	GGTNACC	1 cut(s)	1022
EcoRII	CCWGG	4 cut(s)	14, 477, 686, 963
EcoT38I	GRGCYC	2 cut(s)	431, 1015
Esp3I	CGTCTC	4 cut(s)	65, 217, 262, 688
FaeI	CATG	2 cut(s)	1000, 1259
FaiI	YATR	9 cut(s)	372, 464, 799, 832, 998, 1037, 1046, 1221, 1257
FaqI	GGGAC	3 cut(s)	704, 728, 805
FatI	CATG	2 cut(s)	996, 1255
FauI	CCCGC	2 cut(s)	296, 705
FbaI	TGATCA	1 cut(s)	1047
FblI	GTMKAC	1 cut(s)	798
Fnu4HI	GCNGC	9 cut(s)	115, 169, 200, 292, 345, 604, 730, 1106, 1204
FokI	GGATG	4 cut(s)	38, 308, 334, 1247
FriOI	GRGCYC	2 cut(s)	431, 1015
Fsp4HI	GCNGC	9 cut(s)	115, 169, 200, 292, 345, 604, 730, 1106, 1204
FspBI	CTAG	5 cut(s)	20, 138, 144, 150, 236
GlaI	GCGC	5 cut(s)	148, 390, 566, 857, 988
GluI	GCNGC	9 cut(s)	115, 169, 200, 292, 345, 604, 730, 1106, 1204
GsaI	CCCAGC	1 cut(s)	135
GsuI	CTGGAG	3 cut(s)	176, 203, 899
HaeII	RGCGCY	2 cut(s)	150, 859
HaeIII	GGCC	5 cut(s)	286, 354, 669, 836, 911
HapII	CCGG	7 cut(s)	351, 648, 804, 826, 1027, 1163, 1286
HgaI	GACGC	6 cut(s)	205, 248, 647, 721, 767, 1195
HhaI	GCGC	5 cut(s)	149, 391, 567, 858, 989
Hin1I	GRCGYC	5 cut(s)	74, 240, 639, 759, 1206
Hin1II	CATG	2 cut(s)	1000, 1259
Hin6I	GCGC	5 cut(s)	147, 389, 565, 856, 987
HinP1I	GCGC	5 cut(s)	147, 389, 565, 856, 987
HincII	GTYRAC	2 cut(s)	507, 661
HindII	GTYRAC	2 cut(s)	507, 661
HinfI	GANTC	7 cut(s)	398, 482, 495, 716, 737, 874, 1196
HpaII	CCGG	7 cut(s)	351, 648, 804, 826, 1027, 1163, 1286
HphI	GGTGA	8 cut(s)	69, 301, 586, 637, 910, 1015, 1016, 1177
Hpy166II	GTNNAC	6 cut(s)	507, 661, 766, 799, 901, 1276
Hpy188III	TCNNGA	6 cut(s)	193, 220, 236, 395, 622, 878
Hpy8I	GTNNAC	6 cut(s)	507, 661, 766, 799, 901, 1276
Hpy99I	CGWCG	9 cut(s)	91, 416, 641, 737, 791, 923, 926, 1058, 1211
HpyAV	CCTTC	2 cut(s)	1024, 1240
HpyCH4IV	ACGT	7 cut(s)	74, 366, 591, 861, 1053, 1155, 1272
HpyCH4V	TGCA	2 cut(s)	347, 1306
HpyF10VI	GCNNNNNNNGC	7 cut(s)	159, 168, 177, 416, 767, 902, 1159
HpyF3I	CTNAG	4 cut(s)	69, 281, 1016, 1299
HpySE526I	ACGT	7 cut(s)	74, 366, 591, 861, 1053, 1155, 1272
Hsp92I	GRCGYC	5 cut(s)	74, 240, 639, 759, 1206
Hsp92II	CATG	2 cut(s)	1000, 1259
HspAI	GCGC	5 cut(s)	147, 389, 565, 856, 987
Ksp22I	TGATCA	1 cut(s)	1047
LmnI	GCTCC	3 cut(s)	35, 434, 1227
Lsp1109I	GCAGC	6 cut(s)	126, 180, 186, 331, 716, 1117
LweI	GCATC	3 cut(s)	356, 406, 660
MaeI	CTAG	5 cut(s)	20, 138, 144, 150, 236
MaeII	ACGT	7 cut(s)	74, 366, 591, 861, 1053, 1155, 1272
MaeIII	GTNAC	6 cut(s)	75, 592, 643, 1003, 1022, 1210
MboII	GAAGA	2 cut(s)	715, 1205
MflI	RGATCY	3 cut(s)	624, 721, 822
MhlI	GDGCHC	3 cut(s)	431, 1015, 1155
MluCI	AATT	1 cut(s)	445
MlyI	GAGTC	6 cut(s)	476, 504, 710, 746, 868, 1205
MmeI	TCCRAC	3 cut(s)	110, 477, 800
MseI	TTAA	4 cut(s)	435, 444, 533, 1185
MslI	CAYNNNNRTG	1 cut(s)	1001
MspA1I	CMGCKG	2 cut(s)	729, 896
MspI	CCGG	7 cut(s)	351, 648, 804, 826, 1027, 1163, 1286
MspR9I	CCNGG	6 cut(s)	16, 479, 688, 827, 965, 1287
MvaI	CCWGG	4 cut(s)	16, 479, 688, 965
MvnI	CGCG	3 cut(s)	389, 770, 989
MwoI	GCNNNNNNNGC	7 cut(s)	159, 168, 177, 416, 767, 902, 1159
NciI	CCSGG	2 cut(s)	827, 1287
NheI	GCTAGC	2 cut(s)	19, 149
NlaIII	CATG	2 cut(s)	1000, 1259
NlaIV	GGNNCC	5 cut(s)	8, 287, 430, 626, 824
NmuCI	GTSAC	5 cut(s)	75, 592, 643, 1003, 1022
NspI	RCATGY	1 cut(s)	1259
NspV	TTCGAA	1 cut(s)	1268
PaqCI	CACCTGC	1 cut(s)	320
PcsI	WCGNNNNNNNCGW	7 cut(s)	102, 252, 363, 393, 565, 639, 759
PfeI	GAWTC	1 cut(s)	398
PflFI	GACNNNGTC	1 cut(s)	641
PflMI	CCANNNNNTGG	1 cut(s)	865
PkrI	GCNGC	9 cut(s)	116, 170, 201, 293, 346, 605, 731, 1107, 1205
PleI	GAGTC	6 cut(s)	476, 503, 710, 745, 868, 1204
PmaCI	CACGTG	2 cut(s)	862, 1156
PmlI	CACGTG	2 cut(s)	862, 1156
PpsI	GAGTC	6 cut(s)	476, 503, 710, 745, 868, 1204
Ppu21I	YACGTR	2 cut(s)	862, 1156
Psp6I	CCWGG	4 cut(s)	14, 477, 686, 963
PspCI	CACGTG	2 cut(s)	862, 1156
PspEI	GGTNACC	1 cut(s)	1022
PspFI	CCCAGC	1 cut(s)	131
PspGI	CCWGG	4 cut(s)	14, 477, 686, 963
PspN4I	GGNNCC	5 cut(s)	8, 287, 430, 626, 824
PspPI	GGNCC	4 cut(s)	7, 285, 834, 910
PstNI	CAGNNNCTG	1 cut(s)	199
PsuI	RGATCY	3 cut(s)	624, 721, 822
PsyI	GACNNNGTC	1 cut(s)	641
RsaI	GTAC	1 cut(s)	576
RsaNI	GTAC	1 cut(s)	575
RseI	CAYNNNNRTG	1 cut(s)	1001
SaqAI	TTAA	4 cut(s)	435, 444, 533, 1185
SatI	GCNGC	9 cut(s)	115, 169, 200, 292, 345, 604, 730, 1106, 1204
Sau96I	GGNCC	4 cut(s)	7, 285, 834, 910
SchI	GAGTC	6 cut(s)	476, 504, 710, 746, 868, 1205
ScrFI	CCNGG	6 cut(s)	16, 479, 688, 827, 965, 1287
SduI	GDGCHC	3 cut(s)	431, 1015, 1155
SfaNI	GCATC	3 cut(s)	356, 406, 660
SfuI	TTCGAA	1 cut(s)	1268
SinI	GGWCC	1 cut(s)	7
SmiMI	CAYNNNNRTG	1 cut(s)	1001
Sse9I	AATT	1 cut(s)	445
SspMI	CTAG	5 cut(s)	20, 138, 144, 150, 236
StyD4I	CCNGG	6 cut(s)	14, 477, 686, 825, 963, 1285
TaiI	ACGT	7 cut(s)	77, 369, 594, 864, 1056, 1158, 1275
TaqI	TCGA	6 cut(s)	96, 246, 735, 782, 959, 1268
TasI	AATT	1 cut(s)	445
TauI	GCSGC	3 cut(s)	294, 606, 1206
TfiI	GAWTC	1 cut(s)	398
Tru1I	TTAA	4 cut(s)	435, 444, 533, 1185
Tru9I	TTAA	4 cut(s)	435, 444, 533, 1185
TscAI	CASTG	2 cut(s)	1078, 1098
TseFI	GTSAC	5 cut(s)	75, 592, 643, 1003, 1022
TseI	GCWGC	6 cut(s)	114, 168, 199, 344, 729, 1105
Tsp45I	GTSAC	5 cut(s)	75, 592, 643, 1003, 1022
TspDTI	ATGAA	1 cut(s)	390
TspGWI	ACGGA	6 cut(s)	244, 421, 430, 670, 730, 939
TspRI	CASTG	2 cut(s)	1078, 1098
Tth111I	GACNNNGTC	1 cut(s)	641
Van91I	CCANNNNNTGG	1 cut(s)	865
VpaK11BI	GGWCC	1 cut(s)	7
XbaI	TCTAGA	1 cut(s)	235
XceI	RCATGY	1 cut(s)	1259
XmiI	GTMKAC	1 cut(s)	798
XspI	CTAG	5 cut(s)	20, 138, 144, 150, 236
ZraI	GACGTC	1 cut(s)	75

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.