Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0014209

Physical Location & Seq

Genomic Coordinates Forward (+)

53849000 .. 53850612

Gene Structure 1613 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G027370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGAAGCTCCGATCGTGGTTCTCCGCCACGTGCTCCTCCCACGAATCCACCGTCTCCATCCCCCAACCCCAACCCTCATACCAAAAACGACACGTCGTCTCCGACTCCAGCAGCTCCTCCGATAACCCCAGCAGCTCCTCCAGTACTAGCGACACCTGCAGCACCAGCCTCCATTCCAACCTCTCCCTCCTGACGCTGCCATCTGTCCCCTCCCTTCAGAAGCTCATCTCCCAACTCGACGCCCCAAACCTCTCCGTCTCTCACCTCTGCTTCACCACCCTCACTCCCACCCCCTCCTCCTCCTCCTCTCTCCCGATCACCTGCCTCGCCGTCCACCACAACCGCCTCTACGCCGCCTCCGGCCACCAAATCAACGTCTACGATCGGGCCGACTTCTCCCACCTCGACTCATTCAACGCTCACGATCCCTCCTCGGGCTCCGTGAAGTCCGTCTCGTTTTCCGACGGCAAGGTGTTCACCGCGCACCAGGACTGTAAAATCAAAGTCTGGGAGATAATCCCGGGTAAACGGCACAGACTCCTAACCACCCTCCCCACCGTCAACGACCGTTTGCTTCGTTTTGCGCTCCCCAAGAACTACGTCAGCATACGCCGGCACGTGAAAAGCCTCTGGATCCAGCACGCAGACGCCGTTACCGGCATCGCCGTCAACAACGGCCTCGTCTACTCTGTTTCCTGGGACAAGACCTTGAAGATTTGGCGGGACTCCAACCTCCGCTGCGTGGAGTCCGTCAAGGCGCACGAGGACGCCGTGAGCGCGGTGGCGGTGGCGAAGGACGGGACAGTCTACACCGGGTCGGCGGACCGTCGGATCCGGGTCTGGGCGAGACCGCAAGACGAGAAGCGGCACGTGTTGGTGGCGACGCTGGAGAAGCACAAGTCGGCGGTGAACGCTTTGGCTCTAAACGACGACGGATCGGTGCTGTTCTCGGGGGCCTGTGACCGTTCGATCCTGGTGTGGGAGAGAGAGGACAGCGCGAACCACATGGCGGTTACGGGAGCGTTGAGGGGGCACGGTAAGGCGATACTGTGTTTATTCAACGTCGGGGATTTGCTGCTGAGTGGGTCCGCTGATCGGACGGTGAGGATGTGGCGGCGAGGGGAGGATGGCAGTTATTGCTGTTTGGGGGTTTTGGAGGGACACGTGAAACCGGTGAAGGCGCTGGTGGCGGTTAAGGGAGAGAGTGGTGGCGGCGTCGTTTCGGTTGTGAGTGGGAGTTTAGATGGAGTGGTGAAGGTGTGGCATGTGTCGGTTTCGGAGGCGATTAAAGAACAGAAGATATGTTATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007049 GO:0008092 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016905 GO:0017022 GO:0017076 GO:0018107 GO:0018193 GO:0018210 GO:0019538 GO:0022402 GO:0022411 GO:0030029 GO:0030036 GO:0030554 GO:0030863 GO:0030864 GO:0031033 GO:0031035 GO:0031037 GO:0031038 GO:0032153 GO:0032155 GO:0032553 GO:0032555 GO:0032559 GO:0032984 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045159 GO:0046777 GO:0051301 GO:0061640 GO:0070938 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0099568 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

437

Amino Acids

47.47

Weight (kDa)

8.88

Isoelectric Point (pI)

41.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	105 - 285	7.5e-13	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	214 - 372	6.2e-12	WDHD1 first WD40 domain
WD40_Gbeta	PF25391	214 - 372	2.5e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	214 - 282	1.1e-08	WDR3 first beta-propeller domain
NBCH_WD40	PF20426	221 - 283	7.2e-07	Neurobeachin beta propeller domain
Beta-prop_WDR3_2nd	PF25172	221 - 331	3.4e-14	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	223 - 324	9.2e-12	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	225 - 352	1.1e-08	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	227 - 424	2.3e-10	WDR55
WD40_CDC20-Fz	PF24807	234 - 371	3.2e-13	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	249 - 425	6.2e-14	TEP-1 second beta-propeller
Beta-prop_WDR75_1st	PF23869	253 - 333	2.6e-07	WD repeat-containing protein 75 first beta-propeller
Beta-prop_EML	PF23409	255 - 331	2.4e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	267 - 349	1.4e-07	Prp19 WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	270 - 375	3.4e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5	PF25175	271 - 430	8.2e-23	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	288 - 430	6.8e-17	WDR3 first beta-propeller domain
WD40	PF00400	292 - 327	3.9e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	299 - 371	3.9e-10	THOC3 beta-propeller domain
WD40_Prp19	PF24814	305 - 376	2.9e-06	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24530
fragaria_vesca	FvH4_4g25271
malus_domestica	MD13G1105300.v1.1 MD16G1105400.v1.1
prunus_persica	Prupe.1G241300_v2.0.a1
pyrus_communis	pycom13g09130 pycom16g08970
rosa_chinensis	RchiOBHm_Chr4g0432531
rosa_laevigata	RLG00000006834
rosa_multiflora	Rmu_sc0009980.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373980
rosa_rugosa	Rorug04G0260000.1
rosa_samantha	Rh4AG314800 Rh4BG322500 Rh4CG337700 Rh4DG318500
rosa_wichuraiana	Rw4G027370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	164, 329
AasI	GACNNNNNNGTC	1 cut(s)	803
Acc36I	ACCTGC	2 cut(s)	164, 329
AccI	GTMKAC	3 cut(s)	378, 684, 807
AccII	CGCG	3 cut(s)	482, 779, 998
AclWI	GGATC	7 cut(s)	419, 628, 641, 826, 839, 943, 964
AcoI	YGGCCR	1 cut(s)	361
AcuI	CTGAAG	1 cut(s)	200
AcvI	CACGTG	4 cut(s)	30, 619, 871, 1165
AcyI	GRCGYC	4 cut(s)	240, 648, 768, 1215
AfaI	GTAC	1 cut(s)	145
AfiI	CCNNNNNNNGG	6 cut(s)	434, 742, 841, 979, 1009, 1095
AflIII	ACRYGT	3 cut(s)	91, 870, 1162
AgeI	ACCGGT	1 cut(s)	1171
AgsI	TTSAA	3 cut(s)	415, 712, 1060
AhdI	GACNNNNNGTC	1 cut(s)	95
AjiI	CACGTC	1 cut(s)	94
AjnI	CCWGG	3 cut(s)	486, 695, 972
AluBI	AGCT	4 cut(s)	7, 114, 135, 223
AluI	AGCT	4 cut(s)	7, 114, 135, 223
Alw21I	GWGCWC	1 cut(s)	35
Alw26I	GTCTC	5 cut(s)	58, 103, 262, 457, 841
AlwI	GGATC	7 cut(s)	419, 628, 641, 826, 839, 943, 964
Ama87I	CYCGRG	3 cut(s)	433, 520, 949
AoxI	GGCC	4 cut(s)	361, 387, 676, 954
ApeKI	GCWGC	6 cut(s)	111, 132, 159, 196, 738, 1075
ArsI	GACNNNNNNTTYG	2 cut(s)	36, 68
AsiGI	ACCGGT	1 cut(s)	1171
AspLEI	GCGC	6 cut(s)	484, 586, 760, 779, 998, 1183
AspS9I	GGNCC	4 cut(s)	387, 823, 954, 1086
AsuC2I	CCSGG	4 cut(s)	521, 522, 814, 836
AsuHPI	GGTGA	8 cut(s)	254, 265, 310, 469, 919, 1114, 1186, 1264
AvaI	CYCGRG	3 cut(s)	433, 520, 949
AvaII	GGWCC	2 cut(s)	823, 1086
BaeGI	GKGCMC	1 cut(s)	1035
BamHI	GGATCC	2 cut(s)	633, 831
BanII	GRGCYC	1 cut(s)	440
BauI	CACGAG	1 cut(s)	761
BbrPI	CACGTG	4 cut(s)	30, 619, 871, 1165
Bbv12I	GWGCWC	1 cut(s)	35
BbvI	GCAGC	6 cut(s)	123, 144, 171, 183, 725, 1062
BccI	CCATC	4 cut(s)	65, 208, 1121, 1238
BceAI	ACGGC	7 cut(s)	314, 481, 545, 635, 650, 691, 755
BciT130I	CCWGG	3 cut(s)	488, 697, 974
BcnI	CCSGG	4 cut(s)	521, 522, 814, 836
BcoDI	GTCTC	5 cut(s)	58, 103, 262, 457, 841
BfaI	CTAG	1 cut(s)	147
BfmI	CTRYAG	1 cut(s)	157
BfoI	RGCGCY	1 cut(s)	1184
BfuAI	ACCTGC	2 cut(s)	164, 329
BmcAI	AGTACT	1 cut(s)	145
Bme1390I	CCNGG	7 cut(s)	488, 521, 522, 697, 814, 836, 974
Bme18I	GGWCC	2 cut(s)	823, 1086
BmeRI	GACNNNNNGTC	1 cut(s)	95
BmeT110I	CYCGRG	3 cut(s)	433, 520, 949
BmgBI	CACGTC	1 cut(s)	94
BmgT120I	GGNCC	4 cut(s)	387, 823, 954, 1086
BmiI	GGNNCC	5 cut(s)	439, 635, 833, 955, 1087
BmrFI	CCNGG	7 cut(s)	488, 521, 522, 697, 814, 836, 974
BmsI	GCATC	1 cut(s)	669
BpmI	CTGGAG	3 cut(s)	91, 124, 908
BpuMI	CCSGG	4 cut(s)	521, 522, 814, 836
BsaAI	YACGTR	4 cut(s)	30, 619, 871, 1165
BsaHI	GRCGYC	4 cut(s)	240, 648, 768, 1215
BsaI	GGTCTC	1 cut(s)	841
BsaJI	CCNNGG	3 cut(s)	432, 520, 696
BsaWI	WCCGGW	1 cut(s)	1171
BsaXI	ACNNNNNCTCC	8 cut(s)	83, 113, 268, 298, 341, 371, 413, 443
Bsc4I	CCNNNNNNNGG	6 cut(s)	434, 742, 841, 979, 1009, 1095
Bse118I	RCCGGY	3 cut(s)	612, 656, 1171
Bse1I	ACTGG	1 cut(s)	141
BseBI	CCWGG	3 cut(s)	488, 697, 974
BseDI	CCNNGG	3 cut(s)	432, 520, 696
BseGI	GGATG	3 cut(s)	57, 1113, 1132
BseLI	CCNNNNNNNGG	6 cut(s)	434, 742, 841, 979, 1009, 1095
BseMII	CTCAG	1 cut(s)	1070
BseNI	ACTGG	1 cut(s)	141
BseRI	GAGGAG	8 cut(s)	25, 106, 127, 286, 289, 292, 295, 421
BseSI	GKGCMC	1 cut(s)	1035
BseXI	GCAGC	6 cut(s)	123, 144, 171, 183, 725, 1062
BseYI	CCCAGC	1 cut(s)	128
Bsh1236I	CGCG	3 cut(s)	482, 779, 998
Bsh1285I	CGRYCG	3 cut(s)	14, 385, 568
BshFI	GGCC	4 cut(s)	363, 389, 678, 956
BshTI	ACCGGT	1 cut(s)	1171
BsiEI	CGRYCG	3 cut(s)	14, 385, 568
BsiHKAI	GWGCWC	1 cut(s)	35
BsiHKCI	CYCGRG	3 cut(s)	433, 520, 949
BsiSI	CCGG	7 cut(s)	360, 521, 613, 657, 813, 835, 1172
BslFI	GGGAC	5 cut(s)	191, 713, 737, 814, 1173
BslI	CCNNNNNNNGG	6 cut(s)	434, 742, 841, 979, 1009, 1095
BsmAI	GTCTC	5 cut(s)	58, 103, 262, 457, 841
BsmBI	CGTCTC	4 cut(s)	58, 103, 262, 457
BsmFI	GGGAC	5 cut(s)	191, 713, 737, 814, 1173
BsnI	GGCC	4 cut(s)	363, 389, 678, 956
Bso31I	GGTCTC	1 cut(s)	841
BsoBI	CYCGRG	3 cut(s)	433, 520, 949
Bsp1286I	GDGCHC	3 cut(s)	35, 440, 1035
Bsp143I	GATC	9 cut(s)	11, 315, 382, 424, 633, 831, 935, 969, 1093
BspANI	GGCC	4 cut(s)	363, 389, 678, 956
BspCNI	CTCAG	1 cut(s)	1071
BspFNI	CGCG	3 cut(s)	482, 779, 998
BspLI	GGNNCC	5 cut(s)	439, 635, 833, 955, 1087
BspMAI	CTGCAG	1 cut(s)	161
BspMI	ACCTGC	2 cut(s)	164, 329
BspPI	GGATC	7 cut(s)	419, 628, 641, 826, 839, 943, 964
BspTNI	GGTCTC	1 cut(s)	841
BsrFI	RCCGGY	3 cut(s)	612, 656, 1171
BsrI	ACTGG	1 cut(s)	141
BssAI	RCCGGY	3 cut(s)	612, 656, 1171
BssECI	CCNNGG	3 cut(s)	432, 520, 696
BssMI	GATC	9 cut(s)	11, 315, 382, 424, 633, 831, 935, 969, 1093
BssNI	GRCGYC	4 cut(s)	240, 648, 768, 1215
BssSI	CACGAG	1 cut(s)	761
Bst2BI	CACGAG	1 cut(s)	761
Bst2UI	CCWGG	3 cut(s)	488, 697, 974
BstACI	GRCGYC	4 cut(s)	240, 648, 768, 1215
BstAPI	GCANNNNNTGC	1 cut(s)	1137
BstBAI	YACGTR	4 cut(s)	30, 619, 871, 1165
BstC8I	GCNNGC	2 cut(s)	614, 642
BstDEI	CTNAG	1 cut(s)	1079
BstF5I	GGATG	3 cut(s)	57, 1113, 1132
BstFNI	CGCG	3 cut(s)	482, 779, 998
BstH2I	RGCGCY	1 cut(s)	1184
BstHHI	GCGC	6 cut(s)	484, 586, 760, 779, 998, 1183
BstKTI	GATC	9 cut(s)	14, 318, 385, 427, 636, 834, 938, 972, 1096
BstMAI	GTCTC	5 cut(s)	58, 103, 262, 457, 841
BstMBI	GATC	9 cut(s)	11, 315, 382, 424, 633, 831, 935, 969, 1093
BstMCI	CGRYCG	3 cut(s)	14, 385, 568
BstMWI	GCNNNNNNNGC	7 cut(s)	156, 165, 776, 892, 911, 1137, 1187
BstNI	CCWGG	3 cut(s)	488, 697, 974
BstNSI	RCATGY	1 cut(s)	1268
BstSCI	CCNGG	7 cut(s)	486, 519, 520, 695, 812, 834, 972
BstSFI	CTRYAG	1 cut(s)	157
BstSLI	GKGCMC	1 cut(s)	1035
BstUI	CGCG	3 cut(s)	482, 779, 998
BstV1I	GCAGC	6 cut(s)	123, 144, 171, 183, 725, 1062
BstX2I	RGATCY	2 cut(s)	633, 831
BstYI	RGATCY	2 cut(s)	633, 831
BsuRI	GGCC	4 cut(s)	363, 389, 678, 956
BtgZI	GCGATG	1 cut(s)	646
BtrI	CACGTC	1 cut(s)	94
BtsCI	GGATG	3 cut(s)	57, 1113, 1132
BveI	ACCTGC	2 cut(s)	164, 329
Cac8I	GCNNGC	2 cut(s)	614, 642
CfoI	GCGC	6 cut(s)	484, 586, 760, 779, 998, 1183
Cfr10I	RCCGGY	3 cut(s)	612, 656, 1171
Cfr13I	GGNCC	4 cut(s)	387, 823, 954, 1086
Cfr9I	CCCGGG	1 cut(s)	520
CpoI	CGGWCCG	1 cut(s)	823
CseI	GACGC	6 cut(s)	202, 248, 656, 776, 892, 1204
Csp6I	GTAC	1 cut(s)	144
CspAI	ACCGGT	1 cut(s)	1171
CspI	CGGWCCG	1 cut(s)	823
CviAII	CATG	2 cut(s)	1006, 1265
CviQI	GTAC	1 cut(s)	144
DdeI	CTNAG	1 cut(s)	1079
DpnI	GATC	9 cut(s)	13, 317, 384, 426, 635, 833, 937, 971, 1095
DpnII	GATC	9 cut(s)	11, 315, 382, 424, 633, 831, 935, 969, 1093
DrdI	GACNNNNNNGTC	1 cut(s)	803
DriI	GACNNNNNGTC	1 cut(s)	95
DseDI	GACNNNNNNGTC	1 cut(s)	803
EaeI	YGGCCR	1 cut(s)	361
Eam1105I	GACNNNNNGTC	1 cut(s)	95
EciI	GGCGGA	2 cut(s)	13, 836
Eco24I	GRGCYC	1 cut(s)	440
Eco31I	GGTCTC	1 cut(s)	841
Eco47I	GGWCC	2 cut(s)	823, 1086
Eco57I	CTGAAG	1 cut(s)	200
Eco72I	CACGTG	4 cut(s)	30, 619, 871, 1165
Eco88I	CYCGRG	3 cut(s)	433, 520, 949
EcoO109I	RGGNCCY	1 cut(s)	954
EcoRII	CCWGG	3 cut(s)	486, 695, 972
EcoT38I	GRGCYC	1 cut(s)	440
Esp3I	CGTCTC	4 cut(s)	58, 103, 262, 457
FaeI	CATG	2 cut(s)	1009, 1268
FaiI	YATR	6 cut(s)	79, 608, 1007, 1266, 1303, 1308
FaqI	GGGAC	5 cut(s)	191, 713, 737, 814, 1173
FatI	CATG	2 cut(s)	1005, 1264
FauI	CCCGC	1 cut(s)	714
FblI	GTMKAC	3 cut(s)	378, 684, 807
FokI	GGATG	3 cut(s)	44, 1120, 1139
FriOI	GRGCYC	1 cut(s)	440
FspBI	CTAG	1 cut(s)	147
GlaI	GCGC	6 cut(s)	483, 585, 759, 778, 997, 1182
GsaI	CCCAGC	1 cut(s)	132
GsuI	CTGGAG	3 cut(s)	91, 124, 908
HaeII	RGCGCY	1 cut(s)	1184
HaeIII	GGCC	4 cut(s)	363, 389, 678, 956
HapII	CCGG	7 cut(s)	360, 521, 613, 657, 813, 835, 1172
HgaI	GACGC	6 cut(s)	202, 248, 656, 776, 892, 1204
HhaI	GCGC	6 cut(s)	484, 586, 760, 779, 998, 1183
Hin1I	GRCGYC	4 cut(s)	240, 648, 768, 1215
Hin1II	CATG	2 cut(s)	1009, 1268
Hin6I	GCGC	6 cut(s)	482, 584, 758, 777, 996, 1181
HinP1I	GCGC	6 cut(s)	482, 584, 758, 777, 996, 1181
HincII	GTYRAC	2 cut(s)	562, 670
HindII	GTYRAC	2 cut(s)	562, 670
HinfI	GANTC	6 cut(s)	44, 104, 407, 537, 725, 746
HpaII	CCGG	7 cut(s)	360, 521, 613, 657, 813, 835, 1172
HphI	GGTGA	8 cut(s)	254, 265, 310, 469, 919, 1114, 1186, 1264
Hpy166II	GTNNAC	9 cut(s)	334, 379, 477, 527, 562, 670, 685, 808, 910
Hpy188I	TCNGA	8 cut(s)	11, 103, 121, 219, 463, 831, 1098, 1279
Hpy188III	TCNNGA	4 cut(s)	190, 313, 422, 631
Hpy8I	GTNNAC	9 cut(s)	334, 379, 477, 527, 562, 670, 685, 808, 910
Hpy99I	CGWCG	9 cut(s)	98, 242, 467, 831, 886, 932, 935, 1067, 1220
HpyAV	CCTTC	4 cut(s)	224, 787, 1171, 1249
HpyCH4IV	ACGT	8 cut(s)	29, 93, 375, 600, 618, 870, 1062, 1164
HpyCH4V	TGCA	1 cut(s)	159
HpyF10VI	GCNNNNNNNGC	7 cut(s)	156, 165, 776, 892, 911, 1137, 1187
HpyF3I	CTNAG	1 cut(s)	1079
HpySE526I	ACGT	8 cut(s)	29, 93, 375, 600, 618, 870, 1062, 1164
Hsp92I	GRCGYC	4 cut(s)	240, 648, 768, 1215
Hsp92II	CATG	2 cut(s)	1009, 1268
HspAI	GCGC	6 cut(s)	482, 584, 758, 777, 996, 1181
KroI	GCCGGC	1 cut(s)	612
KroNI	GCCGGC	1 cut(s)	614
Kzo9I	GATC	9 cut(s)	11, 315, 382, 424, 633, 831, 935, 969, 1093
LmnI	GCTCC	7 cut(s)	12, 38, 119, 140, 443, 591, 1019
Lsp1109I	GCAGC	6 cut(s)	123, 144, 171, 183, 725, 1062
LweI	GCATC	1 cut(s)	669
MaeI	CTAG	1 cut(s)	147
MaeII	ACGT	8 cut(s)	29, 93, 375, 600, 618, 870, 1062, 1164
MaeIII	GTNAC	3 cut(s)	652, 959, 1012
MalI	GATC	9 cut(s)	13, 317, 384, 426, 635, 833, 937, 971, 1095
MboI	GATC	9 cut(s)	11, 315, 382, 424, 633, 831, 935, 969, 1093
MboII	GAAGA	2 cut(s)	724, 1309
MflI	RGATCY	2 cut(s)	633, 831
MhlI	GDGCHC	3 cut(s)	35, 440, 1035
MlyI	GAGTC	5 cut(s)	98, 401, 531, 719, 755
MmeI	TCCRAC	5 cut(s)	126, 201, 486, 753, 809
MroNI	GCCGGC	1 cut(s)	612
MseI	TTAA	2 cut(s)	1194, 1287
MspA1I	CMGCKG	2 cut(s)	738, 1091
MspI	CCGG	7 cut(s)	360, 521, 613, 657, 813, 835, 1172
MspR9I	CCNGG	7 cut(s)	488, 521, 522, 697, 814, 836, 974
MvaI	CCWGG	3 cut(s)	488, 697, 974
MvnI	CGCG	3 cut(s)	482, 779, 998
MwoI	GCNNNNNNNGC	7 cut(s)	156, 165, 776, 892, 911, 1137, 1187
NaeI	GCCGGC	1 cut(s)	614
NciI	CCSGG	4 cut(s)	521, 522, 814, 836
NdeII	GATC	9 cut(s)	11, 315, 382, 424, 633, 831, 935, 969, 1093
NgoMIV	GCCGGC	1 cut(s)	612
NlaIII	CATG	2 cut(s)	1009, 1268
NlaIV	GGNNCC	5 cut(s)	439, 635, 833, 955, 1087
NmuCI	GTSAC	1 cut(s)	959
NspI	RCATGY	1 cut(s)	1268
PaqCI	CACCTGC	2 cut(s)	164, 329
PcsI	WCGNNNNNNNCGW	5 cut(s)	48, 99, 387, 648, 768
PdiI	GCCGGC	1 cut(s)	614
PfeI	GAWTC	1 cut(s)	44
PinAI	ACCGGT	1 cut(s)	1171
Ple19I	CGATCG	2 cut(s)	14, 385
PleI	GAGTC	5 cut(s)	98, 401, 531, 719, 754
PmaCI	CACGTG	4 cut(s)	30, 619, 871, 1165
PmlI	CACGTG	4 cut(s)	30, 619, 871, 1165
PpsI	GAGTC	5 cut(s)	98, 401, 531, 719, 754
Ppu21I	YACGTR	4 cut(s)	30, 619, 871, 1165
Psp6I	CCWGG	3 cut(s)	486, 695, 972
PspCI	CACGTG	4 cut(s)	30, 619, 871, 1165
PspFI	CCCAGC	1 cut(s)	128
PspGI	CCWGG	3 cut(s)	486, 695, 972
PspN4I	GGNNCC	5 cut(s)	439, 635, 833, 955, 1087
PspPI	GGNCC	4 cut(s)	387, 823, 954, 1086
PstI	CTGCAG	1 cut(s)	161
PsuI	RGATCY	2 cut(s)	633, 831
PvuI	CGATCG	2 cut(s)	14, 385
RsaI	GTAC	1 cut(s)	145
RsaNI	GTAC	1 cut(s)	144
Rsr2I	CGGWCCG	1 cut(s)	823
RsrII	CGGWCCG	1 cut(s)	823
SaqAI	TTAA	2 cut(s)	1194, 1287
Sau3AI	GATC	9 cut(s)	11, 315, 382, 424, 633, 831, 935, 969, 1093
Sau96I	GGNCC	4 cut(s)	387, 823, 954, 1086
ScaI	AGTACT	1 cut(s)	145
SchI	GAGTC	5 cut(s)	98, 401, 531, 719, 755
ScrFI	CCNGG	7 cut(s)	488, 521, 522, 697, 814, 836, 974
SduI	GDGCHC	3 cut(s)	35, 440, 1035
SfaNI	GCATC	1 cut(s)	669
SfcI	CTRYAG	1 cut(s)	157
SinI	GGWCC	2 cut(s)	823, 1086
SmaI	CCCGGG	1 cut(s)	522
SspMI	CTAG	1 cut(s)	147
StyD4I	CCNGG	7 cut(s)	486, 519, 520, 695, 812, 834, 972
TaiI	ACGT	8 cut(s)	32, 96, 378, 603, 621, 873, 1065, 1167
TaqI	TCGA	3 cut(s)	237, 405, 968
TatI	WGTACW	1 cut(s)	143
TauI	GCSGC	4 cut(s)	356, 868, 1117, 1215
TfiI	GAWTC	1 cut(s)	44
Tru1I	TTAA	2 cut(s)	1194, 1287
Tru9I	TTAA	2 cut(s)	1194, 1287
TseFI	GTSAC	1 cut(s)	959
TseI	GCWGC	6 cut(s)	111, 132, 159, 196, 738, 1075
Tsp45I	GTSAC	1 cut(s)	959
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	5 cut(s)	244, 430, 439, 739, 948
TspMI	CCCGGG	1 cut(s)	520
VpaK11BI	GGWCC	2 cut(s)	823, 1086
XceI	RCATGY	1 cut(s)	1268
XmaI	CCCGGG	1 cut(s)	520
XmiI	GTMKAC	3 cut(s)	378, 684, 807
XspI	CTAG	1 cut(s)	147
ZrmI	AGTACT	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.