MD13G1121300.v1.1

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017879
Physical Location & Seq
Forward (+)
8930668 .. 8934006
3339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1121300.v1.1.491

Sequence Viewer

Length: 387 bp
ATGTTGGACTGGGGCCCGGTTTTCGTGGCGGTGGTGCTGTTCGTTTTGTTATCTCCGGGCTTGCTGTTCCAGTTGCCCGGCCGCCATGGCTTTGTGGAGTTTGGCAGCTTCCACACAAGCGGTGCAGCCATCATGATTCACTCCCTCCTCTACTTTGGTCTCATTTGTGTCTTTTTAATGGCGGATTGGGGGCCGGTGGTGATAGCTGTGGTGCTGTTCGTGCTTCTGAGTCCGGGGCTGCTGTTCCAACTGCCGGGAAGGGGCAAGGTGGTGGAGTTTGGGAACATGCATACGAGCGGGATATCGATCCTCGTCCACACCATCATCTTCTTCGGCCTCCTCACCATCTTCCTCATCGCTGTTGGCGTTCACATCTACACTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

129

Amino Acids

13.87

Weight (kDa)

6.56

Isoelectric Point (pI)

24.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 1 - 61 7.1e-29 Protein of unknown function (DUF3339)
DUF3339 PF11820 60 - 126 2.7e-35 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08391
malus_domestica MD13G1121300.v1.1 MD16G1121600.v1.1
prunus_persica Prupe.1G223900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0429951
rosa_laevigata RLG00000007018
rosa_multiflora Rmu_co8441175.1_g000001 Rmu_ssc0000150.1_g000039
rosa_roxburghii Rroxscaffold_5G00371780
rosa_rugosa Rorug04G0239600
rosa_wichuraiana Rw4G025640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 297
AciI CCGC 5 cut(s) 29, 82, 120, 182, 297
AclWI GGATC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 79
AfiI CCNNNNNNNGG 2 cut(s) 253, 260
AluBI AGCT 2 cut(s) 108, 206
AluI AGCT 2 cut(s) 108, 206
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 301
AoxI GGCC 4 cut(s) 13, 79, 191, 334
ApaI GGGCCC 1 cut(s) 17
ApeKI GCWGC 3 cut(s) 105, 125, 238
AspS9I GGNCC 3 cut(s) 13, 14, 191
AsuC2I CCSGG 5 cut(s) 17, 57, 78, 234, 255
AsuHPI GGTGA 2 cut(s) 211, 334
BaeGI GKGCMC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 17
BbvI GCAGC 3 cut(s) 117, 137, 225
BccI CCATC 3 cut(s) 137, 329, 353
BcnI CCSGG 5 cut(s) 17, 57, 78, 234, 255
BcoDI GTCTC 1 cut(s) 164
BglI GCCNNNNNGGC 1 cut(s) 87
BisI GCNGC 4 cut(s) 82, 106, 126, 239
BlsI GCNGC 4 cut(s) 83, 107, 127, 240
Bme1390I CCNGG 5 cut(s) 17, 57, 78, 234, 255
BmgT120I GGNCC 3 cut(s) 13, 14, 191
BmiI GGNNCC 3 cut(s) 14, 15, 192
BmrFI CCNGG 5 cut(s) 17, 57, 78, 234, 255
BmrI ACTGGG 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 19
BpuMI CCSGG 5 cut(s) 17, 57, 78, 234, 255
Bsa29I ATCGAT 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 85, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 260
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 3 cut(s) 14, 70, 385
Bse8I GATNNNNATC 1 cut(s) 305
BseCI ATCGAT 1 cut(s) 305
BseDI CCNNGG 2 cut(s) 85, 233
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 2 cut(s) 253, 260
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 3 cut(s) 14, 70, 385
BseRI GAGGAG 2 cut(s) 137, 329
BseSI GKGCMC 1 cut(s) 17
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 3 cut(s) 117, 137, 225
BsgI GTGCAG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 4 cut(s) 15, 81, 193, 336
BshVI ATCGAT 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 82
BsiSI CCGG 6 cut(s) 17, 56, 78, 194, 233, 254
BslI CCNNNNNNNGG 2 cut(s) 253, 260
BsmAI GTCTC 1 cut(s) 164
BsnI GGCC 4 cut(s) 15, 81, 193, 336
Bso31I GGTCTC 1 cut(s) 164
Bsp120I GGGCCC 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 1 cut(s) 306
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 5 cut(s) 29, 82, 120, 182, 297
BspANI GGCC 4 cut(s) 15, 81, 193, 336
BspCNI CTCAG 1 cut(s) 219
BspDI ATCGAT 1 cut(s) 305
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 3 cut(s) 14, 15, 192
BspPI GGATC 1 cut(s) 301
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 3 cut(s) 14, 70, 385
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 85, 233
BssMI GATC 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 62
BstDEI CTNAG 1 cut(s) 227
BstDSI CCRYGG 1 cut(s) 85
BstKTI GATC 1 cut(s) 309
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 1 cut(s) 306
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 2 cut(s) 87, 220
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 5 cut(s) 15, 55, 76, 232, 253
BstSLI GKGCMC 1 cut(s) 17
BstV1I GCAGC 3 cut(s) 117, 137, 225
BstZI CGGCCG 1 cut(s) 79
Bsu15I ATCGAT 1 cut(s) 305
BsuRI GGCC 4 cut(s) 15, 81, 193, 336
BsuTUI ATCGAT 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 85
BtgZI GCGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 62
CciI TCATGA 1 cut(s) 132
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 3 cut(s) 13, 14, 191
ClaI ATCGAT 1 cut(s) 305
CviAII CATG 3 cut(s) 86, 133, 286
DdeI CTNAG 1 cut(s) 227
DpnI GATC 1 cut(s) 308
DpnII GATC 1 cut(s) 306
EaeI YGGCCR 1 cut(s) 79
EagI CGGCCG 1 cut(s) 79
EciI GGCGGA 1 cut(s) 197
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 17
Eco31I GGTCTC 1 cut(s) 164
Eco32I GATATC 1 cut(s) 303
Eco52I CGGCCG 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 13
EcoRV GATATC 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 3 cut(s) 89, 136, 289
FaiI YATR 4 cut(s) 87, 134, 287, 291
FatI CATG 3 cut(s) 85, 132, 285
FauI CCCGC 1 cut(s) 290
Fnu4HI GCNGC 4 cut(s) 82, 106, 126, 239
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 4 cut(s) 82, 106, 126, 239
GluI GCNGC 4 cut(s) 82, 106, 126, 239
HaeIII GGCC 4 cut(s) 15, 81, 193, 336
HapII CCGG 6 cut(s) 17, 56, 78, 194, 233, 254
Hin1II CATG 3 cut(s) 89, 136, 289
HinfI GANTC 2 cut(s) 136, 229
HpaII CCGG 6 cut(s) 17, 56, 78, 194, 233, 254
HphI GGTGA 2 cut(s) 211, 334
Hpy166II GTNNAC 2 cut(s) 316, 370
Hpy188I TCNGA 1 cut(s) 228
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 2 cut(s) 316, 370
HpyAV CCTTC 1 cut(s) 252
HpyCH4V TGCA 2 cut(s) 125, 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 220
HpyF3I CTNAG 1 cut(s) 227
Hsp92II CATG 3 cut(s) 89, 136, 289
Kzo9I GATC 1 cut(s) 306
LpnPI CCDG 8 cut(s) 30, 69, 83, 91, 207, 246, 267, 366
Lsp1109I GCAGC 3 cut(s) 117, 137, 225
MalI GATC 1 cut(s) 308
MbiI CCGCTC 1 cut(s) 297
MboI GATC 1 cut(s) 306
MboII GAAGA 3 cut(s) 319, 322, 340
MhlI GDGCHC 1 cut(s) 17
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 6 cut(s) 155, 158, 320, 347, 350, 362
Mph1103I ATGCAT 1 cut(s) 291
MseI TTAA 1 cut(s) 176
MspI CCGG 6 cut(s) 17, 56, 78, 194, 233, 254
MspR9I CCNGG 5 cut(s) 17, 57, 78, 234, 255
MwoI GCNNNNNNNGC 2 cut(s) 87, 220
NciI CCSGG 5 cut(s) 17, 57, 78, 234, 255
NcoI CCATGG 1 cut(s) 85
NdeII GATC 1 cut(s) 306
NlaIII CATG 3 cut(s) 89, 136, 289
NlaIV GGNNCC 3 cut(s) 14, 15, 192
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 1 cut(s) 289
PagI TCATGA 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 4 cut(s) 83, 107, 127, 240
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 14, 15, 192
PspOMI GGGCCC 1 cut(s) 13
PspPI GGNCC 3 cut(s) 13, 14, 191
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 4 cut(s) 82, 106, 126, 239
Sau3AI GATC 1 cut(s) 306
Sau96I GGNCC 3 cut(s) 13, 14, 191
SchI GAGTC 1 cut(s) 238
ScrFI CCNGG 5 cut(s) 17, 57, 78, 234, 255
SduI GDGCHC 1 cut(s) 17
SetI ASST 3 cut(s) 110, 208, 270
SsiI CCGC 5 cut(s) 29, 82, 120, 182, 297
StyD4I CCNGG 5 cut(s) 15, 55, 76, 232, 253
StyI CCWWGG 1 cut(s) 85
TaqI TCGA 1 cut(s) 305
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 1 cut(s) 385
TseI GCWGC 3 cut(s) 105, 125, 238
TspRI CASTG 1 cut(s) 385
XceI RCATGY 1 cut(s) 289
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.