Rw4G025640

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017879
Physical Location & Seq
Reverse (-)
51797918 .. 51799944
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025640.1

Sequence Viewer

Length: 417 bp
ATGTCTGACTGGGGACCGGTGTTTATTGCGGTGGTGCTGTTCGTGCTGTTGTCGCCAGGTCTGCTGTTCCAGGTGCCGGGGCAGCACGGGTGCGTGGAGTTTGGAAGCTTTCACACGAGCGGCGCAGCCATAATGGTTCACTCCCTCCTCTACTTTGCTCTCATTTGCGTCTTCTTGTTGGCCATTAAGGTCCACTTTTACCTTGTAATGGCAGATTGGGGACCAGTGGTGATAGCGGTGGTGTTGTTTGTGCTGTTAAGTCCGGGGCTACTGTTTCAGCTGCCGGGGAAGAGCAGAGTGGTGGAGTTTGGCAACATGCAAACCAGTGGACTATCCATCCTCGTCCACACCATCATCTTCTTTGGCCTCATCACCATCTTCCTCATCGCCATTGGCGTTCACATCTACACTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

138

Amino Acids

14.99

Weight (kDa)

6.56

Isoelectric Point (pI)

23.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 1 - 67 8.1e-33 Protein of unknown function (DUF3339)
DUF3339 PF11820 70 - 136 2.6e-36 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0017879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08391
malus_domestica MD13G1121300.v1.1 MD16G1121600.v1.1
prunus_persica Prupe.1G223900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0429951
rosa_laevigata RLG00000007018
rosa_multiflora Rmu_co8441175.1_g000001 Rmu_ssc0000150.1_g000039
rosa_roxburghii Rroxscaffold_5G00371780
rosa_rugosa Rorug04G0239600
rosa_wichuraiana Rw4G025640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 3 cut(s) 29, 120, 236
AcoI YGGCCR 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 76, 208
AgeI ACCGGT 1 cut(s) 16
AjnI CCWGG 2 cut(s) 55, 69
AluBI AGCT 2 cut(s) 108, 280
AluI AGCT 2 cut(s) 108, 280
AoxI GGCC 2 cut(s) 180, 364
ApeKI GCWGC 3 cut(s) 82, 125, 280
AsiGI ACCGGT 1 cut(s) 16
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 14, 190, 221
AsuC2I CCSGG 3 cut(s) 78, 264, 285
AsuHPI GGTGA 2 cut(s) 241, 364
AvaII GGWCC 3 cut(s) 14, 190, 221
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 73
BauI CACGAG 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 163
BbvI GCAGC 3 cut(s) 94, 137, 267
BccI CCATC 3 cut(s) 344, 359, 383
BciT130I CCWGG 2 cut(s) 57, 71
BcnI CCSGG 3 cut(s) 78, 264, 285
BisI GCNGC 4 cut(s) 83, 121, 126, 281
BlsI GCNGC 4 cut(s) 84, 122, 127, 282
Bme1390I CCNGG 5 cut(s) 57, 71, 78, 264, 285
Bme18I GGWCC 3 cut(s) 14, 190, 221
BmgT120I GGNCC 3 cut(s) 14, 190, 221
BmiI GGNNCC 3 cut(s) 15, 75, 222
BmrFI CCNGG 5 cut(s) 57, 71, 78, 264, 285
BmrI ACTGGG 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 163
BpuMI CCSGG 3 cut(s) 78, 264, 285
BsaJI CCNNGG 3 cut(s) 77, 263, 284
BsaWI WCCGGW 1 cut(s) 16
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 208
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 4 cut(s) 14, 224, 324, 415
BseBI CCWGG 2 cut(s) 57, 71
BseDI CCNNGG 3 cut(s) 77, 263, 284
BseGI GGATG 1 cut(s) 336
BseLI CCNNNNNNNGG 2 cut(s) 76, 208
BseNI ACTGG 4 cut(s) 14, 224, 324, 415
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 3 cut(s) 94, 137, 267
BshFI GGCC 2 cut(s) 182, 366
BshNI GGYRCC 1 cut(s) 73
BshTI ACCGGT 1 cut(s) 16
BsiSI CCGG 4 cut(s) 17, 77, 263, 284
BslFI GGGAC 2 cut(s) 27, 234
BslI CCNNNNNNNGG 2 cut(s) 76, 208
BsmFI GGGAC 2 cut(s) 27, 234
BsnI GGCC 2 cut(s) 182, 366
BspACI CCGC 3 cut(s) 29, 120, 236
BspANI GGCC 2 cut(s) 182, 366
BspLI GGNNCC 3 cut(s) 15, 75, 222
BspQI GCTCTTC 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 73
BsrBI CCGCTC 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 4 cut(s) 14, 224, 324, 415
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 77, 263, 284
BssSI CACGAG 1 cut(s) 115
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 2 cut(s) 57, 71
Bst4CI ACNGT 1 cut(s) 273
Bst6I CTCTTC 1 cut(s) 284
BstF5I GGATG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 125
BstMWI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
BstNI CCWGG 2 cut(s) 57, 71
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 5 cut(s) 55, 69, 76, 262, 283
BstV1I GCAGC 3 cut(s) 94, 137, 267
BstV2I GAAGAC 1 cut(s) 163
BsuRI GGCC 2 cut(s) 182, 366
BtgZI GCGATG 1 cut(s) 370
BtsCI GGATG 1 cut(s) 336
BtsIMutI CAGTG 3 cut(s) 231, 331, 408
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 14, 190, 221
CseI GACGC 1 cut(s) 157
CspAI ACCGGT 1 cut(s) 16
CviAII CATG 1 cut(s) 316
CviJI RGCY 6 cut(s) 108, 128, 182, 268, 280, 366
CviKI_1 RGCY 6 cut(s) 108, 128, 182, 268, 280, 366
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco47I GGWCC 3 cut(s) 14, 190, 221
EcoRII CCWGG 2 cut(s) 55, 69
FaeI CATG 1 cut(s) 319
FaiI YATR 2 cut(s) 131, 317
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 2 cut(s) 27, 234
FatI CATG 1 cut(s) 315
Fnu4HI GCNGC 4 cut(s) 83, 121, 126, 281
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 4 cut(s) 83, 121, 126, 281
GlaI GCGC 1 cut(s) 124
GluI GCNGC 4 cut(s) 83, 121, 126, 281
HaeIII GGCC 2 cut(s) 182, 366
HapII CCGG 4 cut(s) 17, 77, 263, 284
HgaI GACGC 1 cut(s) 157
HhaI GCGC 1 cut(s) 125
Hin1II CATG 1 cut(s) 319
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 106
HpaII CCGG 4 cut(s) 17, 77, 263, 284
HphI GGTGA 2 cut(s) 241, 364
Hpy166II GTNNAC 5 cut(s) 139, 193, 329, 346, 400
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 5 cut(s) 139, 193, 329, 346, 400
HpyCH4III ACNGT 1 cut(s) 273
HpyCH4V TGCA 1 cut(s) 319
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
Hsp92II CATG 1 cut(s) 319
HspAI GCGC 1 cut(s) 123
LguI GCTCTTC 1 cut(s) 284
Lsp1109I GCAGC 3 cut(s) 94, 137, 267
MbiI CCGCTC 1 cut(s) 120
MboII GAAGA 4 cut(s) 163, 301, 349, 370
MlsI TGGCCA 1 cut(s) 182
MluNI TGGCCA 1 cut(s) 182
MnlI CCTC 5 cut(s) 155, 158, 350, 377, 392
Mox20I TGGCCA 1 cut(s) 182
MscI TGGCCA 1 cut(s) 182
MseI TTAA 2 cut(s) 186, 257
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 4 cut(s) 17, 77, 263, 284
MspR9I CCNGG 5 cut(s) 57, 71, 78, 264, 285
MvaI CCWGG 2 cut(s) 57, 71
MwoI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
NciI CCSGG 3 cut(s) 78, 264, 285
NlaIII CATG 1 cut(s) 319
NlaIV GGNNCC 3 cut(s) 15, 75, 222
NspI RCATGY 1 cut(s) 319
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PinAI ACCGGT 1 cut(s) 16
PkrI GCNGC 4 cut(s) 84, 122, 127, 282
Psp6I CCWGG 2 cut(s) 55, 69
PspGI CCWGG 2 cut(s) 55, 69
PspN4I GGNNCC 3 cut(s) 15, 75, 222
PspPI GGNCC 3 cut(s) 14, 190, 221
PvuII CAGCTG 1 cut(s) 280
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 2 cut(s) 186, 257
SatI GCNGC 4 cut(s) 83, 121, 126, 281
Sau96I GGNCC 3 cut(s) 14, 190, 221
ScrFI CCNGG 5 cut(s) 57, 71, 78, 264, 285
SetI ASST 6 cut(s) 61, 75, 110, 192, 204, 282
SinI GGWCC 3 cut(s) 14, 190, 221
SsiI CCGC 3 cut(s) 29, 120, 236
StyD4I CCNGG 5 cut(s) 55, 69, 76, 262, 283
TaaI ACNGT 1 cut(s) 273
TauI GCSGC 1 cut(s) 123
Tru1I TTAA 2 cut(s) 186, 257
Tru9I TTAA 2 cut(s) 186, 257
TscAI CASTG 3 cut(s) 231, 331, 415
TseI GCWGC 3 cut(s) 82, 125, 280
TspRI CASTG 3 cut(s) 231, 331, 415
VpaK11BI GGWCC 3 cut(s) 14, 190, 221
XceI RCATGY 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.