Rroxscaffold_5G00371780

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017879
Physical Location & Seq
Reverse (-)
53325353 .. 53327403
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371780.1

Sequence Viewer

Length: 468 bp
ATGTCTGACTGGGGACCGGTGTTTATTGCGGTGGTGCTGTTCGTGCTGTTGTCGCCGGGTCTGCTGTTCCAGGTGCCGGGGCGGCACGGGTGCGTGGAGTTTGGAAGCTTTCACACGAGCGGCGCAGCCATAATGGTTCACTCCCTCCTCTACTTTGCTCTCATTTGCGTCTTCTTGTTGGCCATTAAGGTCCACTTTTACCTTGTTAAACTTGTTTTGATCACCAATTTGTGTCCTTTGCTGAATGAAGTAGTAGTAATGGCAGATTGGGGACCGGTGGTGGTAGCGGTGGTGTTGTTTGTGCTGTTAAGTCCGGGGCTACTGTTTCAGCTGCCGGGGAAGAGCAGAGTGGTGGAGTTTGGCAACATGCAAACCAGTGGACTATCCATCCTCGTCCACACCATCATCTTCTTTGGCCTCATCACCATCTTCCTCATCGCCATTGGCGTTCACATCTACACTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

155

Amino Acids

16.87

Weight (kDa)

7.0

Isoelectric Point (pI)

22.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 1 - 67 5.8e-33 Protein of unknown function (DUF3339)
DUF3339 PF11820 87 - 153 4.3e-36 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08391
malus_domestica MD13G1121300.v1.1 MD16G1121600.v1.1
prunus_persica Prupe.1G223900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0429951
rosa_laevigata RLG00000007018
rosa_multiflora Rmu_co8441175.1_g000001 Rmu_ssc0000150.1_g000039
rosa_roxburghii Rroxscaffold_5G00371780
rosa_rugosa Rorug04G0239600
rosa_wichuraiana Rw4G025640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 4 cut(s) 29, 82, 120, 287
AcoI YGGCCR 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 76
AgeI ACCGGT 2 cut(s) 16, 274
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 2 cut(s) 108, 331
AluI AGCT 2 cut(s) 108, 331
AoxI GGCC 2 cut(s) 180, 415
ApeKI GCWGC 2 cut(s) 125, 331
AsiGI ACCGGT 2 cut(s) 16, 274
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 14, 190, 272
AsuC2I CCSGG 4 cut(s) 57, 78, 315, 336
AsuHPI GGTGA 2 cut(s) 214, 415
AvaII GGWCC 3 cut(s) 14, 190, 272
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 73
BauI CACGAG 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 137, 318
BccI CCATC 3 cut(s) 395, 410, 434
BciT130I CCWGG 1 cut(s) 71
BclI TGATCA 1 cut(s) 219
BcnI CCSGG 4 cut(s) 57, 78, 315, 336
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 4 cut(s) 83, 121, 126, 332
BlsI GCNGC 4 cut(s) 84, 122, 127, 333
Bme1390I CCNGG 5 cut(s) 57, 71, 78, 315, 336
Bme18I GGWCC 3 cut(s) 14, 190, 272
BmgT120I GGNCC 3 cut(s) 14, 190, 272
BmiI GGNNCC 3 cut(s) 15, 75, 273
BmrFI CCNGG 5 cut(s) 57, 71, 78, 315, 336
BmrI ACTGGG 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 163
BpuMI CCSGG 4 cut(s) 57, 78, 315, 336
BsaJI CCNNGG 3 cut(s) 77, 314, 335
BsaWI WCCGGW 2 cut(s) 16, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 2 cut(s) 16, 274
Bse1I ACTGG 3 cut(s) 14, 375, 466
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 3 cut(s) 77, 314, 335
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 1 cut(s) 76
BseNI ACTGG 3 cut(s) 14, 375, 466
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 2 cut(s) 137, 318
BshFI GGCC 2 cut(s) 182, 417
BshNI GGYRCC 1 cut(s) 73
BshTI ACCGGT 2 cut(s) 16, 274
BsiSI CCGG 6 cut(s) 17, 56, 77, 275, 314, 335
BslFI GGGAC 2 cut(s) 27, 285
BslI CCNNNNNNNGG 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 27, 285
BsnI GGCC 2 cut(s) 182, 417
Bsp143I GATC 1 cut(s) 219
BspACI CCGC 4 cut(s) 29, 82, 120, 287
BspANI GGCC 2 cut(s) 182, 417
BspLI GGNNCC 3 cut(s) 15, 75, 273
BspQI GCTCTTC 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 73
BsrBI CCGCTC 1 cut(s) 120
BsrFI RCCGGY 2 cut(s) 16, 274
BsrI ACTGG 3 cut(s) 14, 375, 466
BssAI RCCGGY 2 cut(s) 16, 274
BssECI CCNNGG 3 cut(s) 77, 314, 335
BssMI GATC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 115
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 324
Bst6I CTCTTC 1 cut(s) 335
BstF5I GGATG 1 cut(s) 387
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 1 cut(s) 222
BstMBI GATC 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 5 cut(s) 55, 69, 76, 313, 334
BstV1I GCAGC 2 cut(s) 137, 318
BstV2I GAAGAC 1 cut(s) 163
BsuRI GGCC 2 cut(s) 182, 417
BtgZI GCGATG 1 cut(s) 421
BtsCI GGATG 1 cut(s) 387
BtsIMutI CAGTG 2 cut(s) 382, 459
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 2 cut(s) 16, 274
Cfr13I GGNCC 3 cut(s) 14, 190, 272
CseI GACGC 1 cut(s) 157
CspAI ACCGGT 2 cut(s) 16, 274
CviAII CATG 1 cut(s) 367
CviJI RGCY 6 cut(s) 108, 128, 182, 319, 331, 417
CviKI_1 RGCY 6 cut(s) 108, 128, 182, 319, 331, 417
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
Eco47I GGWCC 3 cut(s) 14, 190, 272
EcoRII CCWGG 1 cut(s) 69
FaeI CATG 1 cut(s) 370
FaiI YATR 2 cut(s) 131, 368
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 2 cut(s) 27, 285
FatI CATG 1 cut(s) 366
FbaI TGATCA 1 cut(s) 219
Fnu4HI GCNGC 4 cut(s) 83, 121, 126, 332
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 4 cut(s) 83, 121, 126, 332
GlaI GCGC 1 cut(s) 124
GluI GCNGC 4 cut(s) 83, 121, 126, 332
HaeIII GGCC 2 cut(s) 182, 417
HapII CCGG 6 cut(s) 17, 56, 77, 275, 314, 335
HgaI GACGC 1 cut(s) 157
HhaI GCGC 1 cut(s) 125
Hin1II CATG 1 cut(s) 370
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 106
HpaII CCGG 6 cut(s) 17, 56, 77, 275, 314, 335
HphI GGTGA 2 cut(s) 214, 415
Hpy166II GTNNAC 5 cut(s) 139, 193, 380, 397, 451
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 5 cut(s) 139, 193, 380, 397, 451
HpyCH4III ACNGT 1 cut(s) 324
HpyCH4V TGCA 1 cut(s) 370
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
Hsp92II CATG 1 cut(s) 370
HspAI GCGC 1 cut(s) 123
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 1 cut(s) 219
LguI GCTCTTC 1 cut(s) 335
Lsp1109I GCAGC 2 cut(s) 137, 318
MalI GATC 1 cut(s) 221
MbiI CCGCTC 1 cut(s) 120
MboI GATC 1 cut(s) 219
MboII GAAGA 4 cut(s) 163, 352, 400, 421
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 1 cut(s) 226
MluNI TGGCCA 1 cut(s) 182
MnlI CCTC 5 cut(s) 155, 158, 401, 428, 443
Mox20I TGGCCA 1 cut(s) 182
MscI TGGCCA 1 cut(s) 182
MseI TTAA 3 cut(s) 186, 207, 308
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 331
MspI CCGG 6 cut(s) 17, 56, 77, 275, 314, 335
MspR9I CCNGG 5 cut(s) 57, 71, 78, 315, 336
MvaI CCWGG 1 cut(s) 71
MwoI GCNNNNNNNGC 4 cut(s) 43, 52, 61, 82
NciI CCSGG 4 cut(s) 57, 78, 315, 336
NdeII GATC 1 cut(s) 219
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 3 cut(s) 15, 75, 273
NspI RCATGY 1 cut(s) 370
PciSI GCTCTTC 1 cut(s) 335
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PinAI ACCGGT 2 cut(s) 16, 274
PkrI GCNGC 4 cut(s) 84, 122, 127, 333
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 3 cut(s) 15, 75, 273
PspPI GGNCC 3 cut(s) 14, 190, 272
PvuII CAGCTG 1 cut(s) 331
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 3 cut(s) 186, 207, 308
SatI GCNGC 4 cut(s) 83, 121, 126, 332
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 3 cut(s) 14, 190, 272
ScrFI CCNGG 5 cut(s) 57, 71, 78, 315, 336
SetI ASST 5 cut(s) 75, 110, 192, 204, 333
SinI GGWCC 3 cut(s) 14, 190, 272
Sse9I AATT 1 cut(s) 226
SsiI CCGC 4 cut(s) 29, 82, 120, 287
StyD4I CCNGG 5 cut(s) 55, 69, 76, 313, 334
TaaI ACNGT 1 cut(s) 324
TasI AATT 1 cut(s) 226
TauI GCSGC 2 cut(s) 85, 123
Tru1I TTAA 3 cut(s) 186, 207, 308
Tru9I TTAA 3 cut(s) 186, 207, 308
TscAI CASTG 2 cut(s) 382, 466
TseI GCWGC 2 cut(s) 125, 331
TspDTI ATGAA 1 cut(s) 261
TspRI CASTG 2 cut(s) 382, 466
VpaK11BI GGWCC 3 cut(s) 14, 190, 272
XceI RCATGY 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.