MD13G1123800.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015015
Physical Location & Seq
Reverse (-)
9196717 .. 9198304
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1123800.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGATCCCATTATTGCGTTTCTCCTTCTTCTTGCATCTCTTGCCATCCGCCCACCTGCTCTGGCTGCTCGATCAACACCACCAACCGCTCGGATCACCGTTGTGGGAGCAGTTTACTGTGATACCTGTTTAAGCAACAGTTTCTCGAGGAGCAGCTACTTTCTCCCAGGTGTGGACGTCCTCATAAAATGCAAATTCGGAGCAAAATCCCACAAAACAGCATCACAGATGAGCTTCCAGGTCAACAAAACCACAGACAAGTACGGAGTCTACAAACTCGAAATCCCCTCTGTCGATGGAGTCAACTGCATCAGTGGCATTGCCATGAAGTCAATGTGCCAGGCAACCTTGATAGGCAGCTCCTCCTCCGACTGCAATGTCCCCGCCTTAACAACCACCGCAGACGAAATCTCAGTCAAATCGAAACAAGACAATCTCTGCATCTACAGCATGAACGCCTTGAGTTTCCGACCACGTAAGAAAAATCTCACGCTCTGCGGAAACAGGAAGGTGGAGGGTGAATTGGCAACTTCCTTCAATTCCTCCAAGTTCTTCCTCCCTTACTTCCCACCATACGGGTTCTTCCCATGGCCTCGGCCGCCGCCATTTTCTTCCCTGCCCTTTCCTCCTCTGCCTCCATTGCCATTTTTGCCGCCATTCCCGTTCCCGTTCCGGGGCCCGCCATCTTTGCCTTTCCCATTCCCGCAGTTCCCTCCGGCACCAAATCCTTTTGCCCCAAATGCTCCGCCGGCGTTTAATTTGGGGGATCCGAGGACTTGGATTCCTTATATTCCTTCAGTTTTTTCTCCTCCTCCGCCGCCTGCGTTCAGCCTGGGAGATCCCAGAACTTGGATACCATACGTTCCTCCTCCGTCCCCTCTGAACACCCAACAAAACCAGAATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

303

Amino Acids

32.97

Weight (kDa)

9.37

Isoelectric Point (pI)

55.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 34 - 131 5e-19 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13140
fragaria_vesca FvH4_4g23200
malus_domestica MD13G1123800.v1.1 MD16G1124400.v1.1
prunus_persica Prupe.1G221100_v2.0.a1
pyrus_communis pycom13g10830
rosa_chinensis RchiOBHm_Chr4g0429561
rosa_laevigata RLG00000007061
rosa_multiflora Rmu_sc0013305.1_g000002
rosa_roxburghii Rroxscaffold_5G00371260
rosa_rugosa Rorug04G0237100
rosa_samantha Rh4AG292900 Rh4BG298600 Rh4CG314100 Rh4DG295600
rosa_wichuraiana Rw4G025350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 64
AasI GACNNNNNNGTC 1 cut(s) 377
AatII GACGTC 1 cut(s) 180
Acc36I ACCTGC 1 cut(s) 64
AccB1I GGYRCC 1 cut(s) 718
AccB7I CCANNNNNTGG 1 cut(s) 849
AccBSI CCGCTC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 270
AclWI GGATC 5 cut(s) 12, 101, 761, 774, 833
AcoI YGGCCR 1 cut(s) 596
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 780
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 5 cut(s) 172, 575, 673, 674, 849
AgsI TTSAA 1 cut(s) 538
AjnI CCWGG 4 cut(s) 166, 237, 339, 831
AleI CACNNNNGTG 1 cut(s) 101
AluBI AGCT 3 cut(s) 156, 234, 360
AluI AGCT 3 cut(s) 156, 234, 360
AlwI GGATC 5 cut(s) 12, 101, 761, 774, 833
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 3 cut(s) 590, 596, 676
ApaI GGGCCC 1 cut(s) 680
ApeKI GCWGC 3 cut(s) 65, 153, 357
ApoI RAATTY 1 cut(s) 194
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
AspS9I GGNCC 2 cut(s) 676, 677
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 2 cut(s) 88, 530
AvaI CYCGRG 1 cut(s) 145
BaeGI GKGCMC 1 cut(s) 680
BamHI GGATCC 2 cut(s) 4, 766
BanI GGYRCC 1 cut(s) 718
BanII GRGCYC 1 cut(s) 680
BbvI GCAGC 3 cut(s) 52, 165, 369
BccI CCATC 3 cut(s) 53, 290, 691
BciT130I CCWGG 4 cut(s) 168, 239, 341, 833
BciVI GTATCC 1 cut(s) 846
BcnI CCSGG 1 cut(s) 674
BfmI CTRYAG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 64
BfuI GTATCC 1 cut(s) 846
BisI GCNGC 7 cut(s) 66, 154, 358, 599, 602, 653, 818
BlsI GCNGC 7 cut(s) 67, 155, 359, 600, 603, 654, 819
Bme1390I CCNGG 5 cut(s) 168, 239, 341, 674, 833
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 676, 677
BmiI GGNNCC 5 cut(s) 6, 677, 678, 720, 768
BmrFI CCNGG 5 cut(s) 168, 239, 341, 674, 833
BmsI GCATC 4 cut(s) 44, 230, 318, 450
BpuEI CTTGAG 1 cut(s) 481
BpuMI CCSGG 1 cut(s) 674
BsaAI YACGTR 1 cut(s) 476
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 6 cut(s) 166, 587, 593, 673, 770, 832
Bsc4I CCNNNNNNNGG 5 cut(s) 172, 575, 673, 674, 849
Bse118I RCCGGY 1 cut(s) 748
Bse3DI GCAATG 3 cut(s) 318, 382, 638
BseBI CCWGG 4 cut(s) 168, 239, 341, 833
BseDI CCNNGG 6 cut(s) 166, 587, 593, 673, 770, 832
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 5 cut(s) 172, 575, 673, 674, 849
BseMI GCAATG 3 cut(s) 318, 382, 638
BseMII CTCAG 1 cut(s) 426
BseRI GAGGAG 7 cut(s) 163, 352, 355, 618, 798, 801, 858
BseSI GKGCMC 1 cut(s) 680
BseX3I CGGCCG 1 cut(s) 596
BseXI GCAGC 3 cut(s) 52, 165, 369
Bsh1285I CGRYCG 1 cut(s) 599
BshFI GGCC 3 cut(s) 592, 598, 678
BshNI GGYRCC 1 cut(s) 718
BsiEI CGRYCG 1 cut(s) 599
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 3 cut(s) 673, 716, 749
BslFI GGGAC 2 cut(s) 365, 859
BslI CCNNNNNNNGG 5 cut(s) 172, 575, 673, 674, 849
BsmFI GGGAC 2 cut(s) 365, 859
BsnI GGCC 3 cut(s) 592, 598, 678
BsoBI CYCGRG 1 cut(s) 145
Bsp120I GGGCCC 1 cut(s) 676
Bsp1286I GDGCHC 1 cut(s) 680
Bsp143I GATC 5 cut(s) 4, 71, 93, 766, 838
Bsp19I CCATGG 1 cut(s) 587
BspANI GGCC 3 cut(s) 592, 598, 678
BspCNI CTCAG 1 cut(s) 425
BspLI GGNNCC 5 cut(s) 6, 677, 678, 720, 768
BspMI ACCTGC 1 cut(s) 64
BspPI GGATC 5 cut(s) 12, 101, 761, 774, 833
BspT107I GGYRCC 1 cut(s) 718
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 3 cut(s) 318, 382, 638
BsrFI RCCGGY 1 cut(s) 748
BssAI RCCGGY 1 cut(s) 748
BssECI CCNNGG 6 cut(s) 166, 587, 593, 673, 770, 832
BssMI GATC 5 cut(s) 4, 71, 93, 766, 838
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 587
Bst2UI CCWGG 4 cut(s) 168, 239, 341, 833
Bst4CI ACNGT 3 cut(s) 100, 119, 140
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 1 cut(s) 41
BstBAI YACGTR 1 cut(s) 476
BstC8I GCNNGC 3 cut(s) 680, 750, 822
BstDEI CTNAG 1 cut(s) 412
BstDSI CCRYGG 1 cut(s) 587
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 5 cut(s) 7, 74, 96, 769, 841
BstMBI GATC 5 cut(s) 4, 71, 93, 766, 838
BstMCI CGRYCG 1 cut(s) 599
BstNI CCWGG 4 cut(s) 168, 239, 341, 833
BstSCI CCNGG 5 cut(s) 166, 237, 339, 672, 831
BstSFI CTRYAG 1 cut(s) 445
BstSLI GKGCMC 1 cut(s) 680
BstV1I GCAGC 3 cut(s) 52, 165, 369
BstX2I RGATCY 3 cut(s) 4, 766, 838
BstYI RGATCY 3 cut(s) 4, 766, 838
BstZI CGGCCG 1 cut(s) 596
BsuI GTATCC 1 cut(s) 846
BsuRI GGCC 3 cut(s) 592, 598, 678
BtgI CCRYGG 1 cut(s) 587
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 319
BveI ACCTGC 1 cut(s) 64
Cac8I GCNNGC 3 cut(s) 680, 750, 822
Cfr10I RCCGGY 1 cut(s) 748
Cfr13I GGNCC 2 cut(s) 676, 677
Csp6I GTAC 1 cut(s) 262
CviAII CATG 3 cut(s) 325, 451, 588
CviJI RGCY 8 cut(s) 65, 156, 234, 360, 592, 598, 678, 831
CviKI_1 RGCY 8 cut(s) 65, 156, 234, 360, 592, 598, 678, 831
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 1 cut(s) 412
DpnI GATC 5 cut(s) 6, 73, 95, 768, 840
DpnII GATC 5 cut(s) 4, 71, 93, 766, 838
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 596
EagI CGGCCG 1 cut(s) 596
EciI GGCGGA 3 cut(s) 38, 735, 804
EclXI CGGCCG 1 cut(s) 596
Eco130I CCWWGG 1 cut(s) 587
Eco24I GRGCYC 1 cut(s) 680
Eco52I CGGCCG 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 780
Eco88I CYCGRG 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 4 cut(s) 166, 237, 339, 831
EcoT14I CCWWGG 1 cut(s) 587
EcoT38I GRGCYC 1 cut(s) 680
ErhI CCWWGG 1 cut(s) 587
FaeI CATG 3 cut(s) 328, 454, 591
FaiI YATR 8 cut(s) 185, 326, 452, 574, 589, 789, 859, 907
FaqI GGGAC 2 cut(s) 365, 859
FatI CATG 3 cut(s) 324, 450, 587
FauI CCCGC 3 cut(s) 391, 687, 711
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 7 cut(s) 66, 154, 358, 599, 602, 653, 818
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 680
Fsp4HI GCNGC 7 cut(s) 66, 154, 358, 599, 602, 653, 818
GluI GCNGC 7 cut(s) 66, 154, 358, 599, 602, 653, 818
HaeIII GGCC 3 cut(s) 592, 598, 678
HapII CCGG 3 cut(s) 673, 716, 749
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 328, 454, 591
HincII GTYRAC 2 cut(s) 244, 304
HindII GTYRAC 2 cut(s) 244, 304
HinfI GANTC 4 cut(s) 267, 300, 781, 901
HpaII CCGG 3 cut(s) 673, 716, 749
HphI GGTGA 2 cut(s) 88, 530
Hpy166II GTNNAC 5 cut(s) 115, 175, 244, 271, 304
Hpy188I TCNGA 6 cut(s) 93, 200, 370, 470, 771, 882
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 5 cut(s) 115, 175, 244, 271, 304
HpyAV CCTTC 4 cut(s) 35, 502, 544, 804
HpyCH4III ACNGT 3 cut(s) 100, 119, 140
HpyCH4IV ACGT 3 cut(s) 177, 475, 861
HpyCH4V TGCA 5 cut(s) 35, 192, 309, 375, 441
HpyF3I CTNAG 1 cut(s) 412
HpySE526I ACGT 3 cut(s) 177, 475, 861
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 328, 454, 591
KroI GCCGGC 1 cut(s) 748
KroNI GCCGGC 1 cut(s) 750
Kzo9I GATC 5 cut(s) 4, 71, 93, 766, 838
LmnI GCTCC 5 cut(s) 107, 150, 200, 365, 748
Lsp1109I GCAGC 3 cut(s) 52, 165, 369
LweI GCATC 4 cut(s) 44, 230, 318, 450
MaeII ACGT 3 cut(s) 177, 475, 861
MalI GATC 5 cut(s) 6, 73, 95, 768, 840
MbiI CCGCTC 1 cut(s) 89
MboI GATC 5 cut(s) 4, 71, 93, 766, 838
MboII GAAGA 4 cut(s) 20, 544, 574, 603
MflI RGATCY 3 cut(s) 4, 766, 838
MhlI GDGCHC 1 cut(s) 680
MluCI AATT 4 cut(s) 194, 521, 538, 757
MlyI GAGTC 2 cut(s) 276, 309
MmeI TCCRAC 2 cut(s) 393, 493
MreI CGCCGGCG 1 cut(s) 748
MroNI GCCGGC 1 cut(s) 748
MseI TTAA 3 cut(s) 131, 389, 756
MslI CAYNNNNRTG 2 cut(s) 101, 323
MspI CCGG 3 cut(s) 673, 716, 749
MspR9I CCNGG 5 cut(s) 168, 239, 341, 674, 833
MvaI CCWGG 4 cut(s) 168, 239, 341, 833
NaeI GCCGGC 1 cut(s) 750
NciI CCSGG 1 cut(s) 674
NcoI CCATGG 1 cut(s) 587
NdeII GATC 5 cut(s) 4, 71, 93, 766, 838
NgoMIV GCCGGC 1 cut(s) 748
NlaIII CATG 3 cut(s) 328, 454, 591
NlaIV GGNNCC 5 cut(s) 6, 677, 678, 720, 768
NmeAIII GCCGAG 1 cut(s) 574
OliI CACNNNNGTG 1 cut(s) 101
PaeR7I CTCGAG 1 cut(s) 145
PaqCI CACCTGC 1 cut(s) 64
PdiI GCCGGC 1 cut(s) 750
PfeI GAWTC 2 cut(s) 781, 901
PflMI CCANNNNNTGG 1 cut(s) 849
PkrI GCNGC 7 cut(s) 67, 155, 359, 600, 603, 654, 819
PleI GAGTC 2 cut(s) 275, 308
PpsI GAGTC 2 cut(s) 275, 308
Ppu21I YACGTR 1 cut(s) 476
Psp6I CCWGG 4 cut(s) 166, 237, 339, 831
PspGI CCWGG 4 cut(s) 166, 237, 339, 831
PspN4I GGNNCC 5 cut(s) 6, 677, 678, 720, 768
PspOMI GGGCCC 1 cut(s) 676
PspPI GGNCC 2 cut(s) 676, 677
PsuI RGATCY 3 cut(s) 4, 766, 838
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 2 cut(s) 101, 323
SaqAI TTAA 3 cut(s) 131, 389, 756
SatI GCNGC 7 cut(s) 66, 154, 358, 599, 602, 653, 818
Sau3AI GATC 5 cut(s) 4, 71, 93, 766, 838
Sau96I GGNCC 2 cut(s) 676, 677
SchI GAGTC 2 cut(s) 276, 309
ScrFI CCNGG 5 cut(s) 168, 239, 341, 674, 833
SduI GDGCHC 1 cut(s) 680
SfaNI GCATC 4 cut(s) 44, 230, 318, 450
SfcI CTRYAG 1 cut(s) 445
Sfr274I CTCGAG 1 cut(s) 145
SgrAI CRCCGGYG 1 cut(s) 748
SlaI CTCGAG 1 cut(s) 145
SmiMI CAYNNNNRTG 2 cut(s) 101, 323
SmlI CTYRAG 2 cut(s) 145, 460
SmoI CTYRAG 2 cut(s) 145, 460
Sse9I AATT 4 cut(s) 194, 521, 538, 757
StyD4I CCNGG 5 cut(s) 166, 237, 339, 672, 831
StyI CCWWGG 1 cut(s) 587
TaaI ACNGT 3 cut(s) 100, 119, 140
TaiI ACGT 3 cut(s) 180, 478, 864
TaqI TCGA 5 cut(s) 70, 146, 279, 294, 422
TasI AATT 4 cut(s) 194, 521, 538, 757
TauI GCSGC 4 cut(s) 601, 604, 655, 820
TfiI GAWTC 2 cut(s) 781, 901
Tru1I TTAA 3 cut(s) 131, 389, 756
Tru9I TTAA 3 cut(s) 131, 389, 756
TscAI CASTG 1 cut(s) 319
TseI GCWGC 3 cut(s) 65, 153, 357
TspDTI ATGAA 2 cut(s) 341, 467
TspGWI ACGGA 2 cut(s) 279, 861
TspRI CASTG 1 cut(s) 319
Van91I CCANNNNNTGG 1 cut(s) 849
XapI RAATTY 1 cut(s) 194
XhoI CTCGAG 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 270
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.