Rw4G025350

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015015
Physical Location & Seq
Forward (+)
51405145 .. 51406600
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025350.1

Sequence Viewer

Length: 963 bp
ATGCACTCAATGGAGTACCATATATGCTTTGGTCGAAGCTACTTTCGTACCTCTCCCATGGATGCTATTATTTTTCACCTTCTTCTTCTTCTTACATGTTTCCTCACCCACCCAATTGCTCCGGTAGCTCATAAAGCAACAACCCCAACCGCTCGGATCACCGTCGTGGGAGCGGTTTATTGTGATACTTGTTCAAGCAACACATTCTCAAGAAGCAGCTACTTTCTCCCAGGTGTGGATGTTGTGATACAATGCAAGTTCAGAGGGAACTCGGCCAAAGCCTCAGAGGATATGAATTTCTCAGTCAACAAAACCACAAACAAGTATGGAGTTTACAAACTGGAAATACCTTCTGTTGATGGGGTCAACTGCTTAAGTGGTGTGTCAATTAAGTCAATGTGCCAAGCAAGTTTGATATGGAGCTCATCCAAAAGTTGCAATGTTCCTGGTTTAAGAACCACAGCAGATGAGATTTCAGTCAAGTCAAAACAAGATAACCTCTGTATTTACAGCATGAATGCCTTGAGTTACAGGCCACCAAAGAAGAATCTTACCCTTTGTGGAAAGAGGAAGGAAGAGGAATTGGCAAGTTCTTTCAATTCCTCCAAGTTTTTTCTCCCATACTTTCCACCATTCTATCAATTCCCATGGCCTCCTTTGCCTCAATTCCCTTTCCCTCCTCTTCCACCTCTACCATCCTTGCCCCCATTTCCATCTTTTCCATTCCCTCCATCTTTGCCTTTCCCATTTCCAAGTCTACCTCCTTTTCCATCCCCAAATCCTCTTGCCCCAAACACTCCACCTCCATTTAATTTAGGAGACCCAAGAACTTGGATACCTTATATCCCTTCTGTCTTTCCTCCACCTCCTCCCCCACCAGGATTCAATCTCAGAGACCCCAGAACCTGGATACCATATATTCCTCCATCCCCTCCCAATAGGCCTCAAAACCAGAACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

320

Amino Acids

35.67

Weight (kDa)

9.21

Isoelectric Point (pI)

64.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 55 - 150 4.4e-19 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13140
fragaria_vesca FvH4_4g23200
malus_domestica MD13G1123800.v1.1 MD16G1124400.v1.1
prunus_persica Prupe.1G221100_v2.0.a1
pyrus_communis pycom13g10830
rosa_chinensis RchiOBHm_Chr4g0429561
rosa_laevigata RLG00000007061
rosa_multiflora Rmu_sc0013305.1_g000002
rosa_roxburghii Rroxscaffold_5G00371260
rosa_rugosa Rorug04G0237100
rosa_samantha Rh4AG292900 Rh4BG298600 Rh4CG314100 Rh4DG295600
rosa_wichuraiana Rw4G025350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 906
AccBSI CCGCTC 2 cut(s) 152, 173
AccI GTMKAC 1 cut(s) 757
AciI CCGC 2 cut(s) 150, 173
AclWI GGATC 1 cut(s) 164
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 1 cut(s) 295
AfaI GTAC 2 cut(s) 17, 49
AfiI CCNNNNNNNGG 3 cut(s) 235, 878, 906
AflII CTTAAG 1 cut(s) 373
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 3 cut(s) 195, 598, 886
AjnI CCWGG 4 cut(s) 229, 445, 877, 905
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 4 cut(s) 39, 128, 219, 423
AluI AGCT 4 cut(s) 39, 128, 219, 423
Alw21I GWGCWC 1 cut(s) 425
Alw26I GTCTC 2 cut(s) 813, 888
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 906
AoxI GGCC 4 cut(s) 273, 533, 650, 941
ApeKI GCWGC 1 cut(s) 216
ApoI RAATTY 1 cut(s) 295
AsuHPI GGTGA 3 cut(s) 68, 97, 151
BaeI ACNNNNGTAYC 2 cut(s) 31, 64
BanII GRGCYC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 425
BbvI GCAGC 1 cut(s) 228
BccI CCATC 6 cut(s) 353, 703, 721, 739, 778, 934
BciT130I CCWGG 4 cut(s) 231, 447, 879, 907
BciVI GTATCC 2 cut(s) 828, 903
BcoDI GTCTC 2 cut(s) 813, 888
BfrI CTTAAG 1 cut(s) 373
BfuI GTATCC 2 cut(s) 828, 903
BisI GCNGC 1 cut(s) 217
BlsI GCNGC 1 cut(s) 218
Bme1390I CCNGG 4 cut(s) 231, 447, 879, 907
BmrFI CCNGG 4 cut(s) 231, 447, 879, 907
BmsI GCATC 1 cut(s) 52
BpuEI CTTGAG 2 cut(s) 193, 544
BsaI GGTCTC 2 cut(s) 813, 888
BsaJI CCNNGG 3 cut(s) 57, 229, 647
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 2 cut(s) 787, 817
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 878, 906
Bse1I ACTGG 1 cut(s) 345
Bse3DI GCAATG 1 cut(s) 445
BseBI CCWGG 4 cut(s) 231, 447, 879, 907
BseDI CCNNGG 3 cut(s) 57, 229, 647
BseGI GGATG 6 cut(s) 67, 244, 425, 695, 770, 926
BseLI CCNNNNNNNGG 3 cut(s) 235, 878, 906
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 3 cut(s) 297, 315, 904
BseNI ACTGG 1 cut(s) 345
BseRI GAGGAG 2 cut(s) 669, 858
BseXI GCAGC 1 cut(s) 228
BshFI GGCC 4 cut(s) 275, 535, 652, 943
BsiHKAI GWGCWC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 122
BslI CCNNNNNNNGG 3 cut(s) 235, 878, 906
BsmAI GTCTC 2 cut(s) 813, 888
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 4 cut(s) 275, 535, 652, 943
Bso31I GGTCTC 2 cut(s) 813, 888
Bsp1286I GDGCHC 1 cut(s) 425
Bsp143I GATC 1 cut(s) 156
Bsp19I CCATGG 2 cut(s) 57, 647
BspACI CCGC 2 cut(s) 150, 173
BspANI GGCC 4 cut(s) 275, 535, 652, 943
BspCNI CTCAG 3 cut(s) 296, 314, 903
BspPI GGATC 1 cut(s) 164
BspTI CTTAAG 1 cut(s) 373
BspTNI GGTCTC 2 cut(s) 813, 888
BsrBI CCGCTC 2 cut(s) 152, 173
BsrDI GCAATG 1 cut(s) 445
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 3 cut(s) 57, 229, 647
BssMI GATC 1 cut(s) 156
BssT1I CCWWGG 2 cut(s) 57, 647
Bst2UI CCWGG 4 cut(s) 231, 447, 879, 907
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 2 cut(s) 570, 687
BstAFI CTTAAG 1 cut(s) 373
BstDEI CTNAG 3 cut(s) 283, 301, 890
BstDSI CCRYGG 2 cut(s) 57, 647
BstF5I GGATG 6 cut(s) 67, 244, 425, 695, 770, 926
BstKTI GATC 1 cut(s) 159
BstMAI GTCTC 2 cut(s) 813, 888
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 3 cut(s) 125, 134, 658
BstNI CCWGG 4 cut(s) 231, 447, 879, 907
BstNSI RCATGY 1 cut(s) 99
BstSCI CCNGG 4 cut(s) 229, 445, 877, 905
BstV1I GCAGC 1 cut(s) 228
BstXI CCANNNNNNTGG 1 cut(s) 831
BsuI GTATCC 2 cut(s) 828, 903
BsuRI GGCC 4 cut(s) 275, 535, 652, 943
BtgI CCRYGG 2 cut(s) 57, 647
BtsCI GGATG 6 cut(s) 67, 244, 425, 695, 770, 926
CaiI CAGNNNCTG 1 cut(s) 906
Csp6I GTAC 2 cut(s) 16, 48
CviAII CATG 4 cut(s) 58, 96, 514, 648
CviJI RGCY 9 cut(s) 39, 128, 219, 275, 281, 423, 535, 652, 943
CviKI_1 RGCY 9 cut(s) 39, 128, 219, 275, 281, 423, 535, 652, 943
CviQI GTAC 2 cut(s) 16, 48
DdeI CTNAG 3 cut(s) 283, 301, 890
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 570, 687
EarI CTCTTC 2 cut(s) 570, 687
Ecl136II GAGCTC 1 cut(s) 423
Eco130I CCWWGG 2 cut(s) 57, 647
Eco147I AGGCCT 1 cut(s) 943
Eco24I GRGCYC 1 cut(s) 425
Eco31I GGTCTC 2 cut(s) 813, 888
Eco53kI GAGCTC 1 cut(s) 423
EcoICRI GAGCTC 1 cut(s) 423
EcoRII CCWGG 4 cut(s) 229, 445, 877, 905
EcoT14I CCWWGG 2 cut(s) 57, 647
EcoT38I GRGCYC 1 cut(s) 425
ErhI CCWWGG 2 cut(s) 57, 647
FaeI CATG 4 cut(s) 61, 99, 517, 651
FatI CATG 4 cut(s) 57, 95, 513, 647
FblI GTMKAC 1 cut(s) 757
Fnu4HI GCNGC 1 cut(s) 217
FokI GGATG 6 cut(s) 74, 251, 412, 682, 757, 913
FriOI GRGCYC 1 cut(s) 425
Fsp4HI GCNGC 1 cut(s) 217
GluI GCNGC 1 cut(s) 217
HaeIII GGCC 4 cut(s) 275, 535, 652, 943
HapII CCGG 1 cut(s) 122
Hin1II CATG 4 cut(s) 61, 99, 517, 651
HincII GTYRAC 2 cut(s) 307, 367
HindII GTYRAC 2 cut(s) 307, 367
HinfI GANTC 2 cut(s) 547, 882
HpaII CCGG 1 cut(s) 122
HphI GGTGA 3 cut(s) 68, 97, 151
Hpy166II GTNNAC 4 cut(s) 307, 334, 367, 758
Hpy188I TCNGA 4 cut(s) 156, 263, 286, 893
Hpy188III TCNNGA 1 cut(s) 210
Hpy8I GTNNAC 4 cut(s) 307, 334, 367, 758
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 4 cut(s) 89, 360, 565, 858
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4V TGCA 3 cut(s) 4, 255, 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 134, 658
HpyF3I CTNAG 3 cut(s) 283, 301, 890
Hsp92II CATG 4 cut(s) 61, 99, 517, 651
Kzo9I GATC 1 cut(s) 156
LmnI GCTCC 3 cut(s) 124, 170, 420
Lsp1109I GCAGC 1 cut(s) 228
LweI GCATC 1 cut(s) 52
MaeIII GTNAC 1 cut(s) 527
MalI GATC 1 cut(s) 158
MbiI CCGCTC 2 cut(s) 152, 173
MboI GATC 1 cut(s) 156
MboII GAAGA 6 cut(s) 74, 77, 80, 556, 587, 674
MfeI CAATTG 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 425
MluCI AATT 8 cut(s) 114, 295, 387, 581, 598, 641, 665, 811
MseI TTAA 4 cut(s) 374, 390, 452, 810
MslI CAYNNNNRTG 1 cut(s) 164
MspCI CTTAAG 1 cut(s) 373
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 4 cut(s) 231, 447, 879, 907
MunI CAATTG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 4 cut(s) 231, 447, 879, 907
MwoI GCNNNNNNNGC 3 cut(s) 125, 134, 658
NcoI CCATGG 2 cut(s) 57, 647
NdeII GATC 1 cut(s) 156
NlaIII CATG 4 cut(s) 61, 99, 517, 651
NmeAIII GCCGAG 1 cut(s) 251
NspI RCATGY 1 cut(s) 99
OliI CACNNNNGTG 1 cut(s) 164
PceI AGGCCT 1 cut(s) 943
PciI ACATGT 1 cut(s) 95
PctI GAATGC 1 cut(s) 523
PfeI GAWTC 2 cut(s) 547, 882
PflMI CCANNNNNTGG 1 cut(s) 906
PkrI GCNGC 1 cut(s) 218
PscI ACATGT 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 425
Psp6I CCWGG 4 cut(s) 229, 445, 877, 905
PspGI CCWGG 4 cut(s) 229, 445, 877, 905
PstNI CAGNNNCTG 1 cut(s) 906
RsaI GTAC 2 cut(s) 17, 49
RsaNI GTAC 2 cut(s) 16, 48
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 425
SaqAI TTAA 4 cut(s) 374, 390, 452, 810
SatI GCNGC 1 cut(s) 217
Sau3AI GATC 1 cut(s) 156
ScrFI CCNGG 4 cut(s) 231, 447, 879, 907
SduI GDGCHC 1 cut(s) 425
SfaNI GCATC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 3 cut(s) 208, 373, 523
SmoI CTYRAG 3 cut(s) 208, 373, 523
Sse9I AATT 8 cut(s) 114, 295, 387, 581, 598, 641, 665, 811
SseBI AGGCCT 1 cut(s) 943
SsiI CCGC 2 cut(s) 150, 173
SstI GAGCTC 1 cut(s) 425
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 4 cut(s) 229, 445, 877, 905
StyI CCWWGG 2 cut(s) 57, 647
TaaI ACNGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 34
TasI AATT 8 cut(s) 114, 295, 387, 581, 598, 641, 665, 811
TfiI GAWTC 2 cut(s) 547, 882
Tru1I TTAA 4 cut(s) 374, 390, 452, 810
Tru9I TTAA 4 cut(s) 374, 390, 452, 810
TseI GCWGC 1 cut(s) 216
TspDTI ATGAA 2 cut(s) 308, 530
Van91I CCANNNNNTGG 1 cut(s) 906
Vha464I CTTAAG 1 cut(s) 373
XapI RAATTY 1 cut(s) 295
XceI RCATGY 1 cut(s) 99
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.