Rh4AG292900

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0015015
Physical Location & Seq
Forward (+)
60648147 .. 60649405
1259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG292900.1

Sequence Viewer

Length: 906 bp
ATGGATGCTATTATTTTTCACCTTCTTCTTCTTCTTACATGTTTCCTCACCCACCCAATTGCTCCGGTAGCTCATAAAGCAACAACACCAACCGCTCGGATCACCGTCGTGGGAGCGGTTTATTGTGATACTTGTTCAAGCAACACATTCTCAAGAAGCAGCTACTTTCTCCCAGGTGTGGATGTTGTGATACAATGCAAGTTCAGAGGGAACTCGGCCAAAGCCTCAGAGGATATGAATTTCTCAGTCAACAAAACCACAAACAAGTATGGAGTTTACAAACTGGAAATACCTTCTGTTGATGGGGTCAACTGCTTAAGTGGTGTGTCAATTAAGTCAATGTGCCAAGCAAGTTTGATATGGAGCTCATCCAAAAGTTGCAATGTTCCTGGTTTAAGAACCACAGCAGATGAGATTTCAGTCAAGTCAAAACAAGATAACCTCTGTATTTACAGCATGAATGCCTTGAGTTACAGGCCACCAAAGAAGAATCTTACCCTATGTGGAAATAGGAAGGAAGAGGAATTGGCAAGTTCTTTCAATTCCTCCAAGTTTTTTCTCCCATACTTTCCACCATTCTATCGATTCCCATGGCCTCCTTTGCCTCAATTCCCTTTCCCTCCTCTTCCACCTCTACCATCCTTGCCCCCATTTCCATCTTTTCCATTCCCTCCATCTTTGCCTTTCCCATTTCCAAGTCTACCTCCTTTTCCATCCCCAAATCCTCTTGCCCCAAACACTCCACCTCCATTTAATTTAGGAGACCCAAGAACTTGGATACCTTATATCCCTTCTGTCTTTCCTCCACCTCCTCCCCCACCAGGATTCAATCTCAGAGACCCCAGAACCTGGATACCATATATTCCTCCATCCCCTCCCAATAGGCCTCAAAACCAGAACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

301

Amino Acids

33.35

Weight (kDa)

9.22

Isoelectric Point (pI)

60.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 36 - 131 3.9e-19 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13140
fragaria_vesca FvH4_4g23200
malus_domestica MD13G1123800.v1.1 MD16G1124400.v1.1
prunus_persica Prupe.1G221100_v2.0.a1
pyrus_communis pycom13g10830
rosa_chinensis RchiOBHm_Chr4g0429561
rosa_laevigata RLG00000007061
rosa_multiflora Rmu_sc0013305.1_g000002
rosa_roxburghii Rroxscaffold_5G00371260
rosa_rugosa Rorug04G0237100
rosa_samantha Rh4AG292900 Rh4BG298600 Rh4CG314100 Rh4DG295600
rosa_wichuraiana Rw4G025350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 849
AccBSI CCGCTC 2 cut(s) 95, 116
AccI GTMKAC 1 cut(s) 700
AciI CCGC 2 cut(s) 93, 116
AclWI GGATC 1 cut(s) 107
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 1 cut(s) 238
AfiI CCNNNNNNNGG 3 cut(s) 178, 821, 849
AflII CTTAAG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 38
AgsI TTSAA 3 cut(s) 138, 541, 829
AjnI CCWGG 4 cut(s) 172, 388, 820, 848
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 3 cut(s) 71, 162, 366
AluI AGCT 3 cut(s) 71, 162, 366
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 2 cut(s) 756, 831
AlwI GGATC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 849
AoxI GGCC 4 cut(s) 216, 476, 593, 884
ApeKI GCWGC 1 cut(s) 159
ApoI RAATTY 1 cut(s) 238
AsuHPI GGTGA 3 cut(s) 11, 40, 94
BanII GRGCYC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 171
BccI CCATC 6 cut(s) 296, 646, 664, 682, 721, 877
BciT130I CCWGG 4 cut(s) 174, 390, 822, 850
BciVI GTATCC 2 cut(s) 771, 846
BcoDI GTCTC 2 cut(s) 756, 831
BfrI CTTAAG 1 cut(s) 316
BfuI GTATCC 2 cut(s) 771, 846
BisI GCNGC 1 cut(s) 160
BlsI GCNGC 1 cut(s) 161
Bme1390I CCNGG 4 cut(s) 174, 390, 822, 850
BmrFI CCNGG 4 cut(s) 174, 390, 822, 850
BpuEI CTTGAG 2 cut(s) 136, 487
Bsa29I ATCGAT 1 cut(s) 583
BsaI GGTCTC 2 cut(s) 756, 831
BsaJI CCNNGG 2 cut(s) 172, 590
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 730, 760
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 821, 849
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 4 cut(s) 174, 390, 822, 850
BseCI ATCGAT 1 cut(s) 583
BseDI CCNNGG 2 cut(s) 172, 590
BseGI GGATG 6 cut(s) 10, 187, 368, 638, 713, 869
BseLI CCNNNNNNNGG 3 cut(s) 178, 821, 849
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 3 cut(s) 240, 258, 847
BseNI ACTGG 1 cut(s) 288
BseRI GAGGAG 2 cut(s) 612, 801
BseXI GCAGC 1 cut(s) 171
BshFI GGCC 4 cut(s) 218, 478, 595, 886
BshVI ATCGAT 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 3 cut(s) 178, 821, 849
BsmAI GTCTC 2 cut(s) 756, 831
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 4 cut(s) 218, 478, 595, 886
Bso31I GGTCTC 2 cut(s) 756, 831
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 1 cut(s) 99
Bsp19I CCATGG 1 cut(s) 590
BspACI CCGC 2 cut(s) 93, 116
BspANI GGCC 4 cut(s) 218, 478, 595, 886
BspCNI CTCAG 3 cut(s) 239, 257, 846
BspDI ATCGAT 1 cut(s) 583
BspPI GGATC 1 cut(s) 107
BspTI CTTAAG 1 cut(s) 316
BspTNI GGTCTC 2 cut(s) 756, 831
BsrBI CCGCTC 2 cut(s) 95, 116
BsrDI GCAATG 1 cut(s) 388
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 2 cut(s) 172, 590
BssMI GATC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 590
Bst2UI CCWGG 4 cut(s) 174, 390, 822, 850
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 2 cut(s) 513, 630
BstAFI CTTAAG 1 cut(s) 316
BstDEI CTNAG 3 cut(s) 226, 244, 833
BstDSI CCRYGG 1 cut(s) 590
BstF5I GGATG 6 cut(s) 10, 187, 368, 638, 713, 869
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 2 cut(s) 756, 831
BstMBI GATC 1 cut(s) 99
BstMWI GCNNNNNNNGC 3 cut(s) 68, 77, 601
BstNI CCWGG 4 cut(s) 174, 390, 822, 850
BstNSI RCATGY 1 cut(s) 42
BstSCI CCNGG 4 cut(s) 172, 388, 820, 848
BstV1I GCAGC 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 774
Bsu15I ATCGAT 1 cut(s) 583
BsuI GTATCC 2 cut(s) 771, 846
BsuRI GGCC 4 cut(s) 218, 478, 595, 886
BsuTUI ATCGAT 1 cut(s) 583
BtgI CCRYGG 1 cut(s) 590
BtsCI GGATG 6 cut(s) 10, 187, 368, 638, 713, 869
CaiI CAGNNNCTG 1 cut(s) 849
ClaI ATCGAT 1 cut(s) 583
CviAII CATG 3 cut(s) 39, 457, 591
CviJI RGCY 8 cut(s) 71, 162, 218, 224, 366, 478, 595, 886
CviKI_1 RGCY 8 cut(s) 71, 162, 218, 224, 366, 478, 595, 886
DdeI CTNAG 3 cut(s) 226, 244, 833
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 216
Eam1104I CTCTTC 2 cut(s) 513, 630
EarI CTCTTC 2 cut(s) 513, 630
Ecl136II GAGCTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 590
Eco147I AGGCCT 1 cut(s) 886
Eco24I GRGCYC 1 cut(s) 368
Eco31I GGTCTC 2 cut(s) 756, 831
Eco53kI GAGCTC 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 366
EcoRII CCWGG 4 cut(s) 172, 388, 820, 848
EcoT14I CCWWGG 1 cut(s) 590
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 590
FaeI CATG 3 cut(s) 42, 460, 594
FatI CATG 3 cut(s) 38, 456, 590
FblI GTMKAC 1 cut(s) 700
Fnu4HI GCNGC 1 cut(s) 160
FokI GGATG 6 cut(s) 17, 194, 355, 625, 700, 856
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 1 cut(s) 160
GluI GCNGC 1 cut(s) 160
HaeIII GGCC 4 cut(s) 218, 478, 595, 886
HapII CCGG 1 cut(s) 65
Hin1II CATG 3 cut(s) 42, 460, 594
HincII GTYRAC 2 cut(s) 250, 310
HindII GTYRAC 2 cut(s) 250, 310
HinfI GANTC 3 cut(s) 490, 585, 825
HpaII CCGG 1 cut(s) 65
HphI GGTGA 3 cut(s) 11, 40, 94
Hpy166II GTNNAC 4 cut(s) 250, 277, 310, 701
Hpy188I TCNGA 4 cut(s) 99, 206, 229, 836
Hpy188III TCNNGA 1 cut(s) 153
Hpy8I GTNNAC 4 cut(s) 250, 277, 310, 701
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 4 cut(s) 32, 303, 508, 801
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 2 cut(s) 198, 381
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 77, 601
HpyF3I CTNAG 3 cut(s) 226, 244, 833
Hsp92II CATG 3 cut(s) 42, 460, 594
Kzo9I GATC 1 cut(s) 99
LmnI GCTCC 3 cut(s) 67, 113, 363
Lsp1109I GCAGC 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 470
MalI GATC 1 cut(s) 101
MbiI CCGCTC 2 cut(s) 95, 116
MboI GATC 1 cut(s) 99
MboII GAAGA 6 cut(s) 17, 20, 23, 499, 530, 617
MfeI CAATTG 1 cut(s) 57
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 7 cut(s) 57, 238, 330, 524, 541, 608, 754
MseI TTAA 4 cut(s) 317, 333, 395, 753
MslI CAYNNNNRTG 1 cut(s) 107
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 4 cut(s) 174, 390, 822, 850
MunI CAATTG 1 cut(s) 57
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 4 cut(s) 174, 390, 822, 850
MwoI GCNNNNNNNGC 3 cut(s) 68, 77, 601
NcoI CCATGG 1 cut(s) 590
NdeII GATC 1 cut(s) 99
NlaIII CATG 3 cut(s) 42, 460, 594
NmeAIII GCCGAG 1 cut(s) 194
NspI RCATGY 1 cut(s) 42
OliI CACNNNNGTG 1 cut(s) 107
PceI AGGCCT 1 cut(s) 886
PciI ACATGT 1 cut(s) 38
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 3 cut(s) 490, 585, 825
PflMI CCANNNNNTGG 1 cut(s) 849
PkrI GCNGC 1 cut(s) 161
PscI ACATGT 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 368
Psp6I CCWGG 4 cut(s) 172, 388, 820, 848
PspGI CCWGG 4 cut(s) 172, 388, 820, 848
PstNI CAGNNNCTG 1 cut(s) 849
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 4 cut(s) 317, 333, 395, 753
SatI GCNGC 1 cut(s) 160
Sau3AI GATC 1 cut(s) 99
ScrFI CCNGG 4 cut(s) 174, 390, 822, 850
SduI GDGCHC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 3 cut(s) 151, 316, 466
SmoI CTYRAG 3 cut(s) 151, 316, 466
Sse9I AATT 7 cut(s) 57, 238, 330, 524, 541, 608, 754
SseBI AGGCCT 1 cut(s) 886
SsiI CCGC 2 cut(s) 93, 116
SstI GAGCTC 1 cut(s) 368
StuI AGGCCT 1 cut(s) 886
StyD4I CCNGG 4 cut(s) 172, 388, 820, 848
StyI CCWWGG 1 cut(s) 590
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 1 cut(s) 583
TasI AATT 7 cut(s) 57, 238, 330, 524, 541, 608, 754
TfiI GAWTC 3 cut(s) 490, 585, 825
Tru1I TTAA 4 cut(s) 317, 333, 395, 753
Tru9I TTAA 4 cut(s) 317, 333, 395, 753
TseI GCWGC 1 cut(s) 159
TspDTI ATGAA 2 cut(s) 251, 473
Van91I CCANNNNNTGG 1 cut(s) 849
Vha464I CTTAAG 1 cut(s) 316
XapI RAATTY 1 cut(s) 238
XceI RCATGY 1 cut(s) 42
XmiI GTMKAC 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.