MD16G1124400.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015015
Physical Location & Seq
Reverse (-)
9021142 .. 9022857
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1124400.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGATCCAATTATTTCATTTCTTCTGCTTCTTGCATCTCTTGCCATCCGCCCTCCTGCTCTGGCTGCTCGATCCATACAACCAACCGCTCGGATCACTGTTGTGGGAGCTGTTTACTGTGATACCTGTTTAAGCAACAGTTTCTCTAGGAGCAGCTACTTTCTCCCAGGTGCGGACGTCCTCATAAAATGCAAATTCGGAGCAAAATCCCCCAAGTCAGCATCACAGATGAACTTCCATGTCAACAAAACTACAGACAAGTACGGAATGTACAAACTTGAAATCCCTTCTGTCGATGGAGTCAACTGCATCAGTGGCTTTGCCCTGAAGTCAATGTGCCAGGCAACCTTAATAGGCAGCTCCTCCTCCGCCTGCAATGTCCCCGCCTTAAGAACCACCGCAGACGAAATCTCAGTCAAATCGAAACAAGACAACCTCTGCATCTACAACATGAACGCCTTGAGTTTCCGACCACCTAAGAAAAATCTCACATTGTGTGGAAACAGGAAGGTGGAGGGTGAATTGGTAACTTCCTTCAATTCCTCCAAATTCTTCCTCCCTTACTTCCCACCGTATGGGTTCTTCCCTTGGCCTCAGCTGCCGCCATTTTCTTCCCTGCCCTTTCCTCCTCTGCCTCCTTCGCCATTTTTTGCCCCCATTCCCATTCCCATTCCCATTCCGGGGCCCACCGTCTTTGCCTTTCCCATTCCCACAATTCCCTCCGGCACCGAACCCTTTTTCCCCAAATGCTCCGCCAGCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

255

Amino Acids

27.49

Weight (kDa)

9.24

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 34 - 130 1.7e-19 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13140
fragaria_vesca FvH4_4g23200
malus_domestica MD13G1123800.v1.1 MD16G1124400.v1.1
prunus_persica Prupe.1G221100_v2.0.a1
pyrus_communis pycom13g10830
rosa_chinensis RchiOBHm_Chr4g0429561
rosa_laevigata RLG00000007061
rosa_multiflora Rmu_sc0013305.1_g000002
rosa_roxburghii Rroxscaffold_5G00371260
rosa_rugosa Rorug04G0237100
rosa_samantha Rh4AG292900 Rh4BG298600 Rh4CG314100 Rh4DG295600
rosa_wichuraiana Rw4G025350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AccB1I GGYRCC 1 cut(s) 725
AccB7I CCANNNNNTGG 1 cut(s) 575
AccBSI CCGCTC 1 cut(s) 89
AciI CCGC 8 cut(s) 49, 87, 173, 369, 384, 399, 602, 753
AclWI GGATC 3 cut(s) 12, 66, 101
AcsI RAATTY 2 cut(s) 194, 548
AcuI CTGAAG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 495
AfaI GTAC 2 cut(s) 263, 272
AfiI CCNNNNNNNGG 4 cut(s) 172, 575, 680, 681
AflII CTTAAG 1 cut(s) 388
AgsI TTSAA 2 cut(s) 281, 538
AjnI CCWGG 2 cut(s) 166, 339
AleI CACNNNNGTG 1 cut(s) 101
AloI GAACNNNNNNTCC 2 cut(s) 723, 755
AluBI AGCT 4 cut(s) 110, 156, 360, 598
AluI AGCT 4 cut(s) 110, 156, 360, 598
AlwI GGATC 3 cut(s) 12, 66, 101
AoxI GGCC 2 cut(s) 590, 683
ApaI GGGCCC 1 cut(s) 687
ApeKI GCWGC 4 cut(s) 65, 153, 357, 598
ApoI RAATTY 2 cut(s) 194, 548
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
AspS9I GGNCC 2 cut(s) 683, 684
AsuC2I CCSGG 1 cut(s) 681
AsuHPI GGTGA 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 687
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 725
BanII GRGCYC 1 cut(s) 687
BarI GAAGNNNNNNTAC 2 cut(s) 566, 598
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 4 cut(s) 52, 165, 369, 585
BccI CCATC 2 cut(s) 53, 290
BciT130I CCWGG 2 cut(s) 168, 341
BcnI CCSGG 1 cut(s) 681
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 1 cut(s) 252
BfrI CTTAAG 1 cut(s) 388
BisI GCNGC 5 cut(s) 66, 154, 358, 599, 602
BlsI GCNGC 5 cut(s) 67, 155, 359, 600, 603
Bme1390I CCNGG 3 cut(s) 168, 341, 681
BmgT120I GGNCC 2 cut(s) 683, 684
BmiI GGNNCC 4 cut(s) 6, 684, 685, 727
BmrFI CCNGG 3 cut(s) 168, 341, 681
BmsI GCATC 4 cut(s) 44, 230, 318, 450
Bpu10I CCTNAGC 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 481
BpuMI CCSGG 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 166, 587, 680
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 575, 680, 681
Bse3DI GCAATG 1 cut(s) 382
BseBI CCWGG 2 cut(s) 168, 341
BseDI CCNNGG 3 cut(s) 166, 587, 680
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 4 cut(s) 172, 575, 680, 681
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 2 cut(s) 426, 608
BseRI GAGGAG 3 cut(s) 352, 355, 618
BseSI GKGCMC 1 cut(s) 687
BseXI GCAGC 4 cut(s) 52, 165, 369, 585
BshFI GGCC 2 cut(s) 592, 685
BshNI GGYRCC 1 cut(s) 725
BsiSI CCGG 2 cut(s) 680, 723
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 4 cut(s) 172, 575, 680, 681
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 2 cut(s) 592, 685
Bsp120I GGGCCC 1 cut(s) 683
Bsp1286I GDGCHC 1 cut(s) 687
Bsp1407I TGTACA 1 cut(s) 270
Bsp143I GATC 3 cut(s) 4, 71, 93
BspACI CCGC 8 cut(s) 49, 87, 173, 369, 384, 399, 602, 753
BspANI GGCC 2 cut(s) 592, 685
BspCNI CTCAG 2 cut(s) 425, 607
BspLI GGNNCC 4 cut(s) 6, 684, 685, 727
BspPI GGATC 3 cut(s) 12, 66, 101
BspT107I GGYRCC 1 cut(s) 725
BspTI CTTAAG 1 cut(s) 388
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 382
BsrGI TGTACA 1 cut(s) 270
BssECI CCNNGG 3 cut(s) 166, 587, 680
BssMI GATC 3 cut(s) 4, 71, 93
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 587
Bst2UI CCWGG 2 cut(s) 168, 341
Bst4CI ACNGT 5 cut(s) 100, 119, 140, 573, 691
BstACI GRCGYC 1 cut(s) 177
BstAFI CTTAAG 1 cut(s) 388
BstAPI GCANNNNNTGC 1 cut(s) 41
BstAUI TGTACA 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 373, 757
BstDEI CTNAG 3 cut(s) 412, 477, 594
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 3 cut(s) 7, 74, 96
BstMBI GATC 3 cut(s) 4, 71, 93
BstMWI GCNNNNNNNGC 6 cut(s) 41, 65, 315, 598, 640, 756
BstNI CCWGG 2 cut(s) 168, 341
BstSCI CCNGG 3 cut(s) 166, 339, 679
BstSFI CTRYAG 1 cut(s) 252
BstSLI GKGCMC 1 cut(s) 687
BstV1I GCAGC 4 cut(s) 52, 165, 369, 585
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 592, 685
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 96, 319
Cac8I GCNNGC 2 cut(s) 373, 757
Cfr13I GGNCC 2 cut(s) 683, 684
Csp6I GTAC 2 cut(s) 262, 271
CspCI CAANNNNNGTGG 2 cut(s) 676, 711
CviAII CATG 2 cut(s) 239, 451
CviJI RGCY 8 cut(s) 65, 110, 156, 318, 360, 592, 598, 685
CviKI_1 RGCY 8 cut(s) 65, 110, 156, 318, 360, 592, 598, 685
CviQI GTAC 2 cut(s) 262, 271
DdeI CTNAG 3 cut(s) 412, 477, 594
DpnI GATC 3 cut(s) 6, 73, 95
DpnII GATC 3 cut(s) 4, 71, 93
DraIII CACNNNGTG 1 cut(s) 495
EciI GGCGGA 3 cut(s) 38, 358, 742
Eco130I CCWWGG 1 cut(s) 587
Eco24I GRGCYC 1 cut(s) 687
Eco57I CTGAAG 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 683
EcoRII CCWGG 2 cut(s) 166, 339
EcoT14I CCWWGG 1 cut(s) 587
EcoT38I GRGCYC 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 587
FaeI CATG 2 cut(s) 242, 454
FaiI YATR 5 cut(s) 77, 185, 240, 452, 576
FaqI GGGAC 1 cut(s) 365
FatI CATG 2 cut(s) 238, 450
FauI CCCGC 1 cut(s) 391
Fnu4HI GCNGC 5 cut(s) 66, 154, 358, 599, 602
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 687
Fsp4HI GCNGC 5 cut(s) 66, 154, 358, 599, 602
FspBI CTAG 1 cut(s) 147
GluI GCNGC 5 cut(s) 66, 154, 358, 599, 602
HaeIII GGCC 2 cut(s) 592, 685
HapII CCGG 2 cut(s) 680, 723
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 2 cut(s) 242, 454
HincII GTYRAC 2 cut(s) 244, 304
HindII GTYRAC 2 cut(s) 244, 304
HinfI GANTC 1 cut(s) 300
HpaII CCGG 2 cut(s) 680, 723
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 3 cut(s) 115, 244, 304
Hpy188I TCNGA 3 cut(s) 93, 200, 470
Hpy8I GTNNAC 3 cut(s) 115, 244, 304
HpyAV CCTTC 4 cut(s) 297, 502, 544, 648
HpyCH4III ACNGT 5 cut(s) 100, 119, 140, 573, 691
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 5 cut(s) 35, 192, 309, 375, 441
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 65, 315, 598, 640, 756
HpyF3I CTNAG 3 cut(s) 412, 477, 594
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 2 cut(s) 242, 454
Kzo9I GATC 3 cut(s) 4, 71, 93
LmnI GCTCC 5 cut(s) 107, 150, 200, 365, 755
Lsp1109I GCAGC 4 cut(s) 52, 165, 369, 585
LweI GCATC 4 cut(s) 44, 230, 318, 450
MaeI CTAG 1 cut(s) 147
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 526
MalI GATC 3 cut(s) 6, 73, 95
MbiI CCGCTC 1 cut(s) 89
MboI GATC 3 cut(s) 4, 71, 93
MboII GAAGA 4 cut(s) 14, 544, 574, 603
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 6 cut(s) 9, 194, 521, 538, 548, 714
MlyI GAGTC 1 cut(s) 309
MmeI TCCRAC 1 cut(s) 493
MseI TTAA 4 cut(s) 131, 350, 389, 763
MslI CAYNNNNRTG 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 598
MspCI CTTAAG 1 cut(s) 388
MspI CCGG 2 cut(s) 680, 723
MspR9I CCNGG 3 cut(s) 168, 341, 681
MvaI CCWGG 2 cut(s) 168, 341
MwoI GCNNNNNNNGC 6 cut(s) 41, 65, 315, 598, 640, 756
NciI CCSGG 1 cut(s) 681
NdeII GATC 3 cut(s) 4, 71, 93
NlaIII CATG 2 cut(s) 242, 454
NlaIV GGNNCC 4 cut(s) 6, 684, 685, 727
OliI CACNNNNGTG 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 575
PkrI GCNGC 5 cut(s) 67, 155, 359, 600, 603
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 166, 339
PspGI CCWGG 2 cut(s) 166, 339
PspN4I GGNNCC 4 cut(s) 6, 684, 685, 727
PspOMI GGGCCC 1 cut(s) 683
PspPI GGNCC 2 cut(s) 683, 684
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 598
RsaI GTAC 2 cut(s) 263, 272
RsaNI GTAC 2 cut(s) 262, 271
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 4 cut(s) 131, 350, 389, 763
SatI GCNGC 5 cut(s) 66, 154, 358, 599, 602
Sau3AI GATC 3 cut(s) 4, 71, 93
Sau96I GGNCC 2 cut(s) 683, 684
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 3 cut(s) 168, 341, 681
SduI GDGCHC 1 cut(s) 687
SfaNI GCATC 4 cut(s) 44, 230, 318, 450
SfcI CTRYAG 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 388, 460
SmoI CTYRAG 2 cut(s) 388, 460
Sse9I AATT 6 cut(s) 9, 194, 521, 538, 548, 714
SsiI CCGC 8 cut(s) 49, 87, 173, 369, 384, 399, 602, 753
SspMI CTAG 1 cut(s) 147
StyD4I CCNGG 3 cut(s) 166, 339, 679
StyI CCWWGG 1 cut(s) 587
TaaI ACNGT 5 cut(s) 100, 119, 140, 573, 691
TaiI ACGT 1 cut(s) 180
TaqI TCGA 3 cut(s) 70, 294, 422
TasI AATT 6 cut(s) 9, 194, 521, 538, 548, 714
TatI WGTACW 1 cut(s) 270
TauI GCSGC 1 cut(s) 604
Tru1I TTAA 4 cut(s) 131, 350, 389, 763
Tru9I TTAA 4 cut(s) 131, 350, 389, 763
TscAI CASTG 2 cut(s) 103, 319
TseI GCWGC 4 cut(s) 65, 153, 357, 598
TspDTI ATGAA 3 cut(s) 6, 245, 467
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 2 cut(s) 103, 319
Van91I CCANNNNNTGG 1 cut(s) 575
Vha464I CTTAAG 1 cut(s) 388
XapI RAATTY 2 cut(s) 194, 548
XspI CTAG 1 cut(s) 147
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.