MD13G1131400.v1.1

Transaldolase/Fructose-6-phosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011006
Physical Location & Seq
Forward (+)
9986822 .. 9989749
2928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1131400.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGGCTTCCATTTCCAAGCTCTCCAATCCCACCCCCGCTTCCTCCTTGTCCTCCACCTTCAGCCCCAGAACGCCGCAGCCCAAGTGTTTGGTCCGCCTCCTCGCCACCCCCGCCGCCACCTTCAACTCCAAACTCTCCTCCAAGCTCTCCGTACGAACCAAGCCCTCTCTCCGGGGCTCTCTGGTGGTGAGGTGTTCTCAGAGTGATGGGAATGGAAATCCGGTGAAGAGAACTTATCTCCATGATCTGTATGAGAAGGAGGGACAGAGTCCGTGGTATGATAACCTCTGCCGGCCTGTGACGGATCTGCTTCCGCTTATCGCAAGCGGGGTTCGAGGTGTAACCAGTAACCCAGCGATTTTCCAGAAAGCAATCTCAACTTCAAATGCTTACAATGACCAATTCAGGGAACTTGTACAGTCAGGAAAAGACATTGAAAGTGCATATTGGGAACTTGTGGTGAAGGACATTCAAGATGCTTGCAAACTTTTTGAGGCTATCTATGATCAAACAGATGCTGGTGATGGCTACGTTTCTGTTGAAGTTTCTCCCCGACTTGCTGATGACACCCAAGGGACTGTGGATGCTGCAAAATGGCTTCATAAAGTAGTTGCCCGTCCTAATGTCTACATAAAAATTCCTGCCACTGCTCCATGCGTTCCTTCAATTAAGGAAGTTATAGCAAATGGCATAAGTGTCAATGTGACTCTTATATTCTCGCTCAGTAGATATGAGGAGGTGATTGATGCTTACTTGGACGGCCTTGAGGCTTCTGGACTAAGCGACCTCTCCAGAGTCACAAGTGTTGCTTCCTTCTTTGTCAGTAGGGTGGACACCCTCATTGACAAGTTGCTTGAAAAGATTGGAAATCCAGAGGCCCTTGACCTTCGAGGAAAGGCTGCAGTAGCTCAAGCTGCTTTGGCATACCAACTCTACCAAAAGAAATTCTCTGGCCCTAGATGGGAGGCTTTGGTGAAAAAAGGTGCTAAGAAGCAGAGGCTGCTTTGGGCCTCAACCAGCGTCAAGAATCCTGCCTACCCCGATACTTTATATGTTGCTCCTCTCATAGGCCCCGACACGGTTTCAACCATGCCAGACCAAGCTCTTCAAGCATTTGTCGACCATGGCACAGTTTCAAGGACAATTGATTCCAATGTATCCGAAGCTGAAGGCATTTACAGTGCACTGGAGAAGTTAGGAATCGACTGGAGCTACGTTGGGAATCAACTCGAACTTGAGGGAGTGGATTCTTTCAAGAAGAGTTTCGACAGCCTGCTAGACACCCTGCAAGAGAAGGCCAACTCTCTTAAATTGGTTAGTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00030 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00030 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TAL_FSA

Protein Analysis

442

Amino Acids

48.04

Weight (kDa)

6.38

Isoelectric Point (pI)

27.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAL_FSA PF00923 106 - 428 1.2e-77 Transaldolase/Fructose-6-phosphate aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13420
fragaria_vesca FvH4_4g22560 FvH4_4g22560 FvH4_4g22560
malus_domestica MD13G1131400.v1.1 MD16G1141700.v1.1
prunus_persica Prupe.1G214200_v2.0.a1 Prupe.1G214200_v2.0.a1
pyrus_communis pycom13g11470 pycom16g11220
rosa_chinensis RchiOBHm_Chr4g0428801
rosa_laevigata RLG00000007124
rosa_multiflora Rmu_ssc0000273.1_g000001
rosa_roxburghii Rroxscaffold_5G00370480
rosa_rugosa Rorug04G0229100 Rorug04G0229100
rosa_samantha Rh4AG286000 Rh4BG291800 Rh4CG307800
rosa_wichuraiana Rw4G024790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 627, 1119
AciI CCGC 7 cut(s) 36, 74, 94, 111, 114, 314, 327
AclWI GGATC 1 cut(s) 312
AcsI RAATTY 2 cut(s) 636, 944
AcuI CTGAAG 2 cut(s) 43, 1188
AfaI GTAC 2 cut(s) 153, 417
AfiI CCNNNNNNNGG 5 cut(s) 171, 406, 961, 1067, 1078
AluBI AGCT 7 cut(s) 19, 145, 908, 914, 1103, 1166, 1212
AluI AGCT 7 cut(s) 19, 145, 908, 914, 1103, 1166, 1212
Alw21I GWGCWC 1 cut(s) 1186
Alw44I GTGCAC 1 cut(s) 1182
AlwI GGATC 1 cut(s) 312
AlwNI CAGNNNCTG 2 cut(s) 518, 1000
AoxI GGCC 7 cut(s) 293, 760, 876, 952, 1008, 1069, 1296
ApaLI GTGCAC 1 cut(s) 1182
ApeKI GCWGC 5 cut(s) 76, 587, 899, 914, 1000
ApoI RAATTY 2 cut(s) 636, 944
Asp700I GAANNNNTTC 1 cut(s) 1262
AspS9I GGNCC 5 cut(s) 91, 877, 953, 1008, 1070
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 6 cut(s) 199, 235, 472, 533, 751, 985
AvaII GGWCC 1 cut(s) 91
BaeGI GKGCMC 1 cut(s) 1186
BanII GRGCYC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 1186
BbvI GCAGC 5 cut(s) 88, 574, 886, 901, 987
BccI CCATC 3 cut(s) 200, 518, 954
BceAI ACGGC 1 cut(s) 775
BciVI GTATCC 1 cut(s) 1168
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 173
BfaI CTAG 2 cut(s) 957, 1277
BfmI CTRYAG 1 cut(s) 900
BfuI GTATCC 1 cut(s) 1168
BisI GCNGC 7 cut(s) 74, 77, 114, 588, 900, 915, 1001
BlsI GCNGC 7 cut(s) 75, 78, 115, 589, 901, 916, 1002
Bme1390I CCNGG 1 cut(s) 173
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 5 cut(s) 91, 877, 953, 1008, 1070
BmiI GGNNCC 1 cut(s) 1072
BmrFI CCNGG 1 cut(s) 173
BmsI GCATC 4 cut(s) 466, 505, 574, 736
BplI GAGNNNNNCTC 2 cut(s) 181, 213
BpmI CTGGAG 3 cut(s) 775, 1208, 1228
BpuEI CTTGAG 3 cut(s) 785, 894, 1256
BpuMI CCSGG 1 cut(s) 173
BsaJI CCNNGG 4 cut(s) 172, 272, 571, 1123
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 406, 961, 1067, 1078
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 3 cut(s) 345, 1191, 1211
BseDI CCNNGG 4 cut(s) 172, 272, 571, 1123
BseGI GGATG 1 cut(s) 589
BseLI CCNNNNNNNGG 5 cut(s) 171, 406, 961, 1067, 1078
BseMII CTCAG 2 cut(s) 212, 736
BseNI ACTGG 3 cut(s) 345, 1191, 1211
BseRI GAGGAG 4 cut(s) 89, 127, 749, 1050
BseSI GKGCMC 1 cut(s) 1186
BseXI GCAGC 5 cut(s) 88, 574, 886, 901, 987
BseYI CCCAGC 1 cut(s) 352
BshFI GGCC 7 cut(s) 295, 762, 878, 954, 1010, 1071, 1298
BsiHKAI GWGCWC 1 cut(s) 1186
BsiSI CCGG 3 cut(s) 172, 221, 292
BsiWI CGTACG 1 cut(s) 151
BslFI GGGAC 2 cut(s) 276, 589
BslI CCNNNNNNNGG 5 cut(s) 171, 406, 961, 1067, 1078
BsmFI GGGAC 2 cut(s) 276, 589
BsnI GGCC 7 cut(s) 295, 762, 878, 954, 1010, 1071, 1298
Bsp1286I GDGCHC 2 cut(s) 179, 1186
Bsp1407I TGTACA 1 cut(s) 415
Bsp143I GATC 3 cut(s) 244, 304, 505
Bsp19I CCATGG 1 cut(s) 1123
BspACI CCGC 7 cut(s) 36, 74, 94, 111, 114, 314, 327
BspANI GGCC 7 cut(s) 295, 762, 878, 954, 1010, 1071, 1298
BspCNI CTCAG 2 cut(s) 211, 735
BspLI GGNNCC 1 cut(s) 1072
BspMAI CTGCAG 1 cut(s) 904
BspPI GGATC 1 cut(s) 312
BspQI GCTCTTC 1 cut(s) 1110
BsrFI RCCGGY 1 cut(s) 291
BsrGI TGTACA 1 cut(s) 415
BsrI ACTGG 3 cut(s) 345, 1191, 1211
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 4 cut(s) 172, 272, 571, 1123
BssMI GATC 3 cut(s) 244, 304, 505
BssT1I CCWWGG 2 cut(s) 571, 1123
Bst4CI ACNGT 5 cut(s) 420, 580, 1081, 1132, 1181
Bst6I CTCTTC 3 cut(s) 221, 1110, 1253
BstAPI GCANNNNNTGC 1 cut(s) 1000
BstAUI TGTACA 1 cut(s) 415
BstC8I GCNNGC 4 cut(s) 293, 325, 481, 1274
BstDEI CTNAG 4 cut(s) 198, 722, 779, 987
BstDSI CCRYGG 2 cut(s) 272, 1123
BstENI CCTNNNNNAGG 1 cut(s) 1065
BstF5I GGATG 1 cut(s) 589
BstKTI GATC 3 cut(s) 247, 307, 508
BstMBI GATC 3 cut(s) 244, 304, 505
BstMWI GCNNNNNNNGC 6 cut(s) 110, 905, 914, 920, 1000, 1109
BstSCI CCNGG 1 cut(s) 171
BstSFI CTRYAG 1 cut(s) 900
BstSLI GKGCMC 1 cut(s) 1186
BstV1I GCAGC 5 cut(s) 88, 574, 886, 901, 987
BstX2I RGATCY 1 cut(s) 304
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 1 cut(s) 304
BsuI GTATCC 1 cut(s) 1168
BsuRI GGCC 7 cut(s) 295, 762, 878, 954, 1010, 1071, 1298
BtgI CCRYGG 2 cut(s) 272, 1123
BtsCI GGATG 1 cut(s) 589
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 3 cut(s) 645, 1184, 1186
Cac8I GCNNGC 4 cut(s) 293, 325, 481, 1274
CaiI CAGNNNCTG 2 cut(s) 518, 1000
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 5 cut(s) 91, 877, 953, 1008, 1070
CseI GACGC 1 cut(s) 1009
Csp6I GTAC 2 cut(s) 152, 416
CviAII CATG 4 cut(s) 242, 654, 1090, 1124
CviQI GTAC 2 cut(s) 152, 416
DdeI CTNAG 4 cut(s) 198, 722, 779, 987
DpnI GATC 3 cut(s) 246, 306, 507
DpnII GATC 3 cut(s) 244, 304, 505
Eam1104I CTCTTC 3 cut(s) 221, 1110, 1253
EarI CTCTTC 3 cut(s) 221, 1110, 1253
EciI GGCGGA 1 cut(s) 83
Eco130I CCWWGG 2 cut(s) 571, 1123
Eco24I GRGCYC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 91
Eco57I CTGAAG 2 cut(s) 43, 1188
EcoNI CCTNNNNNAGG 1 cut(s) 1065
EcoO109I RGGNCCY 2 cut(s) 877, 1070
EcoT14I CCWWGG 2 cut(s) 571, 1123
EcoT38I GRGCYC 1 cut(s) 179
ErhI CCWWGG 2 cut(s) 571, 1123
FaeI CATG 4 cut(s) 245, 657, 1093, 1127
FalI AAGNNNNNCTT 2 cut(s) 793, 825
FaqI GGGAC 2 cut(s) 276, 589
FatI CATG 4 cut(s) 241, 653, 1089, 1123
FauI CCCGC 3 cut(s) 43, 118, 320
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 2 cut(s) 627, 1119
Fnu4HI GCNGC 7 cut(s) 74, 77, 114, 588, 900, 915, 1001
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 7 cut(s) 74, 77, 114, 588, 900, 915, 1001
FspBI CTAG 2 cut(s) 957, 1277
GluI GCNGC 7 cut(s) 74, 77, 114, 588, 900, 915, 1001
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 3 cut(s) 775, 1208, 1228
HaeIII GGCC 7 cut(s) 295, 762, 878, 954, 1010, 1071, 1298
HapII CCGG 3 cut(s) 172, 221, 292
HgaI GACGC 1 cut(s) 1009
Hin1II CATG 4 cut(s) 245, 657, 1093, 1127
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HinfI GANTC 8 cut(s) 268, 706, 795, 1027, 1148, 1200, 1222, 1247
HpaII CCGG 3 cut(s) 172, 221, 292
HphI GGTGA 6 cut(s) 199, 235, 472, 533, 751, 985
Hpy166II GTNNAC 4 cut(s) 628, 832, 1120, 1184
Hpy188I TCNGA 2 cut(s) 201, 1162
Hpy188III TCNNGA 8 cut(s) 364, 423, 473, 774, 792, 872, 1024, 1255
Hpy8I GTNNAC 4 cut(s) 628, 832, 1120, 1184
HpyAV CCTTC 9 cut(s) 67, 130, 250, 457, 672, 823, 896, 1163, 1288
HpyCH4III ACNGT 5 cut(s) 420, 580, 1081, 1132, 1181
HpyCH4IV ACGT 2 cut(s) 531, 1215
HpyCH4V TGCA 6 cut(s) 443, 483, 590, 902, 1184, 1288
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 905, 914, 920, 1000, 1109
HpyF3I CTNAG 4 cut(s) 198, 722, 779, 987
HpySE526I ACGT 2 cut(s) 531, 1215
Hsp92II CATG 4 cut(s) 245, 657, 1093, 1127
KroI GCCGGC 1 cut(s) 291
KroNI GCCGGC 1 cut(s) 293
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 3 cut(s) 244, 304, 505
LguI GCTCTTC 1 cut(s) 1110
LmnI GCTCC 3 cut(s) 655, 1063, 1209
Lsp1109I GCAGC 5 cut(s) 88, 574, 886, 901, 987
LweI GCATC 4 cut(s) 466, 505, 574, 736
MaeI CTAG 2 cut(s) 957, 1277
MaeII ACGT 2 cut(s) 531, 1215
MaeIII GTNAC 5 cut(s) 298, 340, 347, 703, 796
MalI GATC 3 cut(s) 246, 306, 507
MboI GATC 3 cut(s) 244, 304, 505
MboII GAAGA 3 cut(s) 238, 1097, 1270
MfeI CAATTG 1 cut(s) 1143
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 2 cut(s) 179, 1186
MluCI AATT 6 cut(s) 401, 636, 666, 944, 1143, 1310
MlyI GAGTC 3 cut(s) 277, 700, 804
MroNI GCCGGC 1 cut(s) 291
MroXI GAANNNNTTC 1 cut(s) 1262
MseI TTAA 2 cut(s) 669, 1308
MspI CCGG 3 cut(s) 172, 221, 292
MspR9I CCNGG 1 cut(s) 173
MunI CAATTG 1 cut(s) 1143
MwoI GCNNNNNNNGC 6 cut(s) 110, 905, 914, 920, 1000, 1109
NaeI GCCGGC 1 cut(s) 293
NciI CCSGG 1 cut(s) 173
NcoI CCATGG 1 cut(s) 1123
NdeII GATC 3 cut(s) 244, 304, 505
NgoMIV GCCGGC 1 cut(s) 291
NlaIII CATG 4 cut(s) 245, 657, 1093, 1127
NlaIV GGNNCC 1 cut(s) 1072
NmuCI GTSAC 3 cut(s) 298, 703, 796
PciSI GCTCTTC 1 cut(s) 1110
PdiI GCCGGC 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 1262
PfeI GAWTC 5 cut(s) 1027, 1148, 1200, 1222, 1247
Pfl23II CGTACG 1 cut(s) 151
PflFI GACNNNGTC 1 cut(s) 267
PkrI GCNGC 7 cut(s) 75, 78, 115, 589, 901, 916, 1002
PleI GAGTC 3 cut(s) 276, 700, 803
PpsI GAGTC 3 cut(s) 276, 700, 803
PspFI CCCAGC 1 cut(s) 352
PspLI CGTACG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 1072
PspPI GGNCC 5 cut(s) 91, 877, 953, 1008, 1070
PstI CTGCAG 1 cut(s) 904
PstNI CAGNNNCTG 2 cut(s) 518, 1000
PsuI RGATCY 1 cut(s) 304
PsyI GACNNNGTC 1 cut(s) 267
RsaI GTAC 2 cut(s) 153, 417
RsaNI GTAC 2 cut(s) 152, 416
SalI GTCGAC 1 cut(s) 1118
SapI GCTCTTC 1 cut(s) 1110
SaqAI TTAA 2 cut(s) 669, 1308
SatI GCNGC 7 cut(s) 74, 77, 114, 588, 900, 915, 1001
Sau3AI GATC 3 cut(s) 244, 304, 505
Sau96I GGNCC 5 cut(s) 91, 877, 953, 1008, 1070
SchI GAGTC 3 cut(s) 277, 700, 804
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 2 cut(s) 179, 1186
SfaNI GCATC 4 cut(s) 466, 505, 574, 736
SfcI CTRYAG 1 cut(s) 900
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 3 cut(s) 764, 909, 1235
SmoI CTYRAG 3 cut(s) 764, 909, 1235
Sse9I AATT 6 cut(s) 401, 636, 666, 944, 1143, 1310
SsiI CCGC 7 cut(s) 36, 74, 94, 111, 114, 314, 327
SspMI CTAG 2 cut(s) 957, 1277
StyD4I CCNGG 1 cut(s) 171
StyI CCWWGG 2 cut(s) 571, 1123
TaaI ACNGT 5 cut(s) 420, 580, 1081, 1132, 1181
TaiI ACGT 2 cut(s) 534, 1218
TaqI TCGA 6 cut(s) 334, 889, 1119, 1203, 1230, 1266
TasI AATT 6 cut(s) 401, 636, 666, 944, 1143, 1310
TatI WGTACW 1 cut(s) 415
TauI GCSGC 2 cut(s) 76, 116
TfiI GAWTC 5 cut(s) 1027, 1148, 1200, 1222, 1247
Tru1I TTAA 2 cut(s) 669, 1308
Tru9I TTAA 2 cut(s) 669, 1308
TscAI CASTG 3 cut(s) 652, 1186, 1191
TseFI GTSAC 3 cut(s) 298, 703, 796
TseI GCWGC 5 cut(s) 76, 587, 899, 914, 1000
Tsp45I GTSAC 3 cut(s) 298, 703, 796
TspDTI ATGAA 1 cut(s) 590
TspGWI ACGGA 3 cut(s) 139, 261, 317
TspRI CASTG 3 cut(s) 652, 1186, 1191
Tth111I GACNNNGTC 1 cut(s) 267
VneI GTGCAC 1 cut(s) 1182
VpaK11BI GGWCC 1 cut(s) 91
XagI CCTNNNNNAGG 1 cut(s) 1065
XapI RAATTY 2 cut(s) 636, 944
XmiI GTMKAC 2 cut(s) 627, 1119
XmnI GAANNNNTTC 1 cut(s) 1262
XspI CTAG 2 cut(s) 957, 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.