Rroxscaffold_5G00370480

Transaldolase/Fructose-6-phosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011006
Physical Location & Seq
Reverse (-)
52188960 .. 52192155
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00370480.1

Sequence Viewer

Length: 1329 bp
ATGGGTTCCATTTCCAAGCTCTCCAATCCCTGCCCTGCTTCTTCTCTCTCCTCCACCTTCAACCCAAAAGCTTCCAACCCCAAGTGCTTGATCGGCTTCCTCACCACCTCCCCCAACAGCTTCGCCTCCAAGGTCTCGTCATCCAAGCTCTCCGTACGAAGCAAGCCCTCTCTCAGAGGCCCTTTGATAGTGAGATGTGCTCAGGGTGAAGGAAATGGAAGCCCAACAAAGAAGACATATCTCCATGAGCTTTATGACAAGGAAGGGCAGAGTCCGTGGTATGATAACCTCTGCAGACCAGTTACAGATCTGCTTCCGCTCATTGATAGCGGGGTTAGAGGTGTTACTAGTAACCCAGCGATTTTCCAGAAAGCAATATCAACTTCTAATGCTTACAATGATCAATTCAGGGGACTTGTACAGTCAGGGAAAGACATTGAAACTGCATATTGGGAACTTGTGGTGAAGGACATTCAAGATGCTTGCAAGCTTTTTGAGTCAATCTATGATCAAACTGATGCTGCTGATGGCTATGTTTCTGTTGAAGTTTCTCCCAGACTTGCTGATGACACTGAAGGGACTGTAGAGGCTGCAAAATGGCTACATAAAGTTGTTTCCCGACCCAATGTCTACATAAAGATTCCCGCTACTGCTCCATGCATCCCTTCTATTAAGGAAGTTATAGCAAATGGCATTAGTGTCAATGTGACTCTTATATTCTCTCTCAGTAGATATGAGGCAGTCATTGATGCGTATTTGGATGGCCTTGAGGCTTCCGGCCTAAACGACCTCTCCAGAGTCACAAGTGTTGCTTCCTTCTTTGTTAGTAGGGTTGACACCCTTATTGACAAGTTGCTTGAAAAGATTGGAACTCCAGAGGCCCTTGACCTTCGAGGAAAGGCTGCAGTAGCTCAAGCCGCTTTAGCATACCAACTATACCAGAAGAAATTCTCAGGTCCCAGATGGGAGGCTTTGGTGAAGAAGGGTGCTAAGAAGCAGAGGCTGCTGTGGGCCTCAACCAGTGTCAAGAATCCCGCCTACCCTGATACTTTATATGTTGCTCCTCTCATTGGTCCTGACACGGTTTCAACCATGCCCGACCAAGCTCTGCAAGCCTTCCTCGATCATGGAACTGTTTCAAGGACAATTGATTCCAATGTATCAGAGGCTGAAGGCATTTACAGTGCCCTTGAGCATCTTGGAATTGACTGGAGCTATGTCGGTAACCAACTCGAACTTGAAGGAGTGGACTCTTTCAAGAAGAGTTTTGACAGCCTGCTTGATACTCTGCAAGAGAAGGCCAACTCTCTTAAATTGGTTAGTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00030 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00030 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TAL_FSA

Protein Analysis

442

Amino Acids

47.99

Weight (kDa)

5.94

Isoelectric Point (pI)

27.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAL_FSA PF00923 107 - 429 6.9e-78 Transaldolase/Fructose-6-phosphate aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13420
fragaria_vesca FvH4_4g22560 FvH4_4g22560 FvH4_4g22560
malus_domestica MD13G1131400.v1.1 MD16G1141700.v1.1
prunus_persica Prupe.1G214200_v2.0.a1 Prupe.1G214200_v2.0.a1
pyrus_communis pycom13g11470 pycom16g11220
rosa_chinensis RchiOBHm_Chr4g0428801
rosa_laevigata RLG00000007124
rosa_multiflora Rmu_ssc0000273.1_g000001
rosa_roxburghii Rroxscaffold_5G00370480
rosa_rugosa Rorug04G0229100 Rorug04G0229100
rosa_samantha Rh4AG286000 Rh4BG291800 Rh4CG307800
rosa_wichuraiana Rw4G024790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 319
AccI GTMKAC 1 cut(s) 630
AciI CCGC 5 cut(s) 317, 330, 645, 918, 1035
AcsI RAATTY 1 cut(s) 947
AcuI CTGAAG 2 cut(s) 594, 1191
AfaI GTAC 2 cut(s) 156, 420
AfiI CCNNNNNNNGG 1 cut(s) 1070
AgsI TTSAA 9 cut(s) 61, 440, 476, 545, 860, 1089, 1140, 1241, 1258
AhdI GACNNNNNGTC 1 cut(s) 626
AhlI ACTAGT 1 cut(s) 347
AluBI AGCT 9 cut(s) 19, 71, 120, 148, 250, 490, 911, 1106, 1215
AluI AGCT 9 cut(s) 19, 71, 120, 148, 250, 490, 911, 1106, 1215
Alw21I GWGCWC 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 139
AlwNI CAGNNNCTG 2 cut(s) 1003, 1169
AoxI GGCC 6 cut(s) 178, 763, 778, 879, 1011, 1299
ApeKI GCWGC 4 cut(s) 521, 590, 902, 1003
ApoI RAATTY 1 cut(s) 947
Asp700I GAANNNNTTC 2 cut(s) 947, 1135
AspS9I GGNCC 5 cut(s) 179, 880, 956, 1011, 1073
AsuHPI GGTGA 4 cut(s) 94, 218, 475, 988
AvaII GGWCC 2 cut(s) 956, 1073
BaeGI GKGCMC 1 cut(s) 1189
BbsI GAAGAC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 4 cut(s) 508, 577, 889, 990
BccI CCATC 3 cut(s) 521, 755, 957
BclI TGATCA 2 cut(s) 400, 508
BcoDI GTCTC 1 cut(s) 139
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 3 cut(s) 292, 582, 903
BglII AGATCT 1 cut(s) 307
BisI GCNGC 5 cut(s) 522, 591, 903, 918, 1004
BlsI GCNGC 5 cut(s) 523, 592, 904, 919, 1005
Bme18I GGWCC 2 cut(s) 956, 1073
BmeRI GACNNNNNGTC 1 cut(s) 626
BmgT120I GGNCC 5 cut(s) 179, 880, 956, 1011, 1073
BmiI GGNNCC 2 cut(s) 7, 958
BmsI GCATC 5 cut(s) 469, 508, 669, 739, 1204
BpiI GAAGAC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpmI CTGGAG 3 cut(s) 778, 858, 1231
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 3 cut(s) 788, 897, 1211
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 129, 275
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bsc4I CCNNNNNNNGG 1 cut(s) 1070
Bse1I ACTGG 3 cut(s) 299, 1020, 1214
BseDI CCNNGG 2 cut(s) 129, 275
BseGI GGATG 3 cut(s) 140, 660, 766
BseLI CCNNNNNNNGG 1 cut(s) 1070
BseMII CTCAG 4 cut(s) 187, 215, 739, 966
BseNI ACTGG 3 cut(s) 299, 1020, 1214
BseRI GAGGAG 2 cut(s) 40, 1053
BseSI GKGCMC 1 cut(s) 1189
BseXI GCAGC 4 cut(s) 508, 577, 889, 990
BseYI CCCAGC 1 cut(s) 355
BshFI GGCC 6 cut(s) 180, 765, 780, 881, 1013, 1301
BsiHKAI GWGCWC 1 cut(s) 202
BsiSI CCGG 1 cut(s) 777
BsiWI CGTACG 1 cut(s) 154
BslFI GGGAC 3 cut(s) 426, 592, 942
BslI CCNNNNNNNGG 1 cut(s) 1070
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 3 cut(s) 426, 592, 942
BsnI GGCC 6 cut(s) 180, 765, 780, 881, 1013, 1301
Bso31I GGTCTC 1 cut(s) 139
Bsp1286I GDGCHC 2 cut(s) 202, 1189
Bsp1407I TGTACA 1 cut(s) 418
Bsp143I GATC 5 cut(s) 90, 307, 400, 508, 1123
BspACI CCGC 5 cut(s) 317, 330, 645, 918, 1035
BspANI GGCC 6 cut(s) 180, 765, 780, 881, 1013, 1301
BspCNI CTCAG 4 cut(s) 186, 214, 738, 965
BspLI GGNNCC 2 cut(s) 7, 958
BspMAI CTGCAG 2 cut(s) 296, 907
BspTNI GGTCTC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 418
BsrI ACTGG 3 cut(s) 299, 1020, 1214
BssECI CCNNGG 2 cut(s) 129, 275
BssMI GATC 5 cut(s) 90, 307, 400, 508, 1123
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 5 cut(s) 423, 583, 1084, 1135, 1184
Bst6I CTCTTC 1 cut(s) 1256
BstAPI GCANNNNNTGC 1 cut(s) 1003
BstAUI TGTACA 1 cut(s) 418
BstC8I GCNNGC 5 cut(s) 164, 484, 488, 1113, 1277
BstDEI CTNAG 5 cut(s) 173, 201, 725, 952, 990
BstDSI CCRYGG 1 cut(s) 275
BstEII GGTNACC 1 cut(s) 1223
BstF5I GGATG 3 cut(s) 140, 660, 766
BstKTI GATC 5 cut(s) 93, 310, 403, 511, 1126
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 5 cut(s) 90, 307, 400, 508, 1123
BstMWI GCNNNNNNNGC 6 cut(s) 93, 908, 917, 923, 1003, 1112
BstPI GGTNACC 1 cut(s) 1223
BstSFI CTRYAG 3 cut(s) 292, 582, 903
BstSLI GKGCMC 1 cut(s) 1189
BstV1I GCAGC 4 cut(s) 508, 577, 889, 990
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 6 cut(s) 180, 765, 780, 881, 1013, 1301
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 3 cut(s) 140, 660, 766
BtsIMutI CAGTG 3 cut(s) 570, 1027, 1189
Cac8I GCNNGC 5 cut(s) 164, 484, 488, 1113, 1277
CaiI CAGNNNCTG 2 cut(s) 1003, 1169
Cfr13I GGNCC 5 cut(s) 179, 880, 956, 1011, 1073
Csp6I GTAC 2 cut(s) 155, 419
CviAII CATG 4 cut(s) 245, 657, 1093, 1127
CviQI GTAC 2 cut(s) 155, 419
DdeI CTNAG 5 cut(s) 173, 201, 725, 952, 990
DpnI GATC 5 cut(s) 92, 309, 402, 510, 1125
DpnII GATC 5 cut(s) 90, 307, 400, 508, 1123
DriI GACNNNNNGTC 1 cut(s) 626
Eam1104I CTCTTC 1 cut(s) 1256
Eam1105I GACNNNNNGTC 1 cut(s) 626
EarI CTCTTC 1 cut(s) 1256
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 956, 1073
Eco57I CTGAAG 2 cut(s) 594, 1191
Eco91I GGTNACC 1 cut(s) 1223
EcoO109I RGGNCCY 3 cut(s) 179, 880, 956
EcoO65I GGTNACC 1 cut(s) 1223
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 662
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 4 cut(s) 248, 660, 1096, 1130
FalI AAGNNNNNCTT 2 cut(s) 796, 828
FaqI GGGAC 3 cut(s) 426, 592, 942
FatI CATG 4 cut(s) 244, 656, 1092, 1126
FauI CCCGC 3 cut(s) 323, 652, 1042
FbaI TGATCA 2 cut(s) 400, 508
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 5 cut(s) 522, 591, 903, 918, 1004
FokI GGATG 3 cut(s) 127, 647, 773
Fsp4HI GCNGC 5 cut(s) 522, 591, 903, 918, 1004
FspBI CTAG 1 cut(s) 348
GluI GCNGC 5 cut(s) 522, 591, 903, 918, 1004
GsaI CCCAGC 1 cut(s) 359
GsuI CTGGAG 3 cut(s) 778, 858, 1231
HaeIII GGCC 6 cut(s) 180, 765, 780, 881, 1013, 1301
HapII CCGG 1 cut(s) 777
Hin1II CATG 4 cut(s) 248, 660, 1096, 1130
HincII GTYRAC 1 cut(s) 835
HindII GTYRAC 1 cut(s) 835
HindIII AAGCTT 2 cut(s) 69, 488
HinfI GANTC 8 cut(s) 271, 497, 640, 709, 798, 1030, 1151, 1250
HpaII CCGG 1 cut(s) 777
HphI GGTGA 4 cut(s) 94, 218, 475, 988
Hpy166II GTNNAC 3 cut(s) 631, 835, 1249
Hpy188I TCNGA 2 cut(s) 176, 1165
Hpy188III TCNNGA 8 cut(s) 367, 476, 618, 795, 875, 1027, 1076, 1258
Hpy8I GTNNAC 3 cut(s) 631, 835, 1249
HpyCH4III ACNGT 5 cut(s) 423, 583, 1084, 1135, 1184
HpyCH4V TGCA 8 cut(s) 294, 446, 486, 593, 660, 905, 1111, 1291
HpyF10VI GCNNNNNNNGC 6 cut(s) 93, 908, 917, 923, 1003, 1112
HpyF3I CTNAG 5 cut(s) 173, 201, 725, 952, 990
Hsp92II CATG 4 cut(s) 248, 660, 1096, 1130
Ksp22I TGATCA 2 cut(s) 400, 508
Kzo9I GATC 5 cut(s) 90, 307, 400, 508, 1123
LmnI GCTCC 3 cut(s) 658, 1066, 1212
Lsp1109I GCAGC 4 cut(s) 508, 577, 889, 990
LweI GCATC 5 cut(s) 469, 508, 669, 739, 1204
MaeI CTAG 1 cut(s) 348
MaeIII GTNAC 6 cut(s) 301, 343, 350, 706, 799, 1223
MalI GATC 5 cut(s) 92, 309, 402, 510, 1125
MbiI CCGCTC 1 cut(s) 319
MboI GATC 5 cut(s) 90, 307, 400, 508, 1123
MboII GAAGA 5 cut(s) 33, 244, 955, 991, 1273
MfeI CAATTG 1 cut(s) 1146
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 2 cut(s) 202, 1189
MluCI AATT 5 cut(s) 404, 947, 1146, 1203, 1313
MlyI GAGTC 5 cut(s) 280, 506, 703, 807, 1244
MmeI TCCRAC 1 cut(s) 99
Mph1103I ATGCAT 1 cut(s) 662
MroXI GAANNNNTTC 2 cut(s) 947, 1135
MseI TTAA 2 cut(s) 672, 1311
MspI CCGG 1 cut(s) 777
MunI CAATTG 1 cut(s) 1146
MwoI GCNNNNNNNGC 6 cut(s) 93, 908, 917, 923, 1003, 1112
NdeII GATC 5 cut(s) 90, 307, 400, 508, 1123
NlaIII CATG 4 cut(s) 248, 660, 1096, 1130
NlaIV GGNNCC 2 cut(s) 7, 958
NmuCI GTSAC 2 cut(s) 706, 799
NsiI ATGCAT 1 cut(s) 662
PdmI GAANNNNTTC 2 cut(s) 947, 1135
PfeI GAWTC 3 cut(s) 640, 1030, 1151
Pfl23II CGTACG 1 cut(s) 154
PkrI GCNGC 5 cut(s) 523, 592, 904, 919, 1005
PleI GAGTC 5 cut(s) 279, 505, 703, 806, 1244
PpsI GAGTC 5 cut(s) 279, 505, 703, 806, 1244
PpuMI RGGWCCY 1 cut(s) 956
Psp5II RGGWCCY 1 cut(s) 956
PspEI GGTNACC 1 cut(s) 1223
PspFI CCCAGC 1 cut(s) 355
PspLI CGTACG 1 cut(s) 154
PspN4I GGNNCC 2 cut(s) 7, 958
PspPI GGNCC 5 cut(s) 179, 880, 956, 1011, 1073
PspPPI RGGWCCY 1 cut(s) 956
PstI CTGCAG 2 cut(s) 296, 907
PstNI CAGNNNCTG 2 cut(s) 1003, 1169
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 2 cut(s) 156, 420
RsaNI GTAC 2 cut(s) 155, 419
SaqAI TTAA 2 cut(s) 672, 1311
SatI GCNGC 5 cut(s) 522, 591, 903, 918, 1004
Sau3AI GATC 5 cut(s) 90, 307, 400, 508, 1123
Sau96I GGNCC 5 cut(s) 179, 880, 956, 1011, 1073
SchI GAGTC 5 cut(s) 280, 506, 703, 807, 1244
SduI GDGCHC 2 cut(s) 202, 1189
SfaNI GCATC 5 cut(s) 469, 508, 669, 739, 1204
SfcI CTRYAG 3 cut(s) 292, 582, 903
SinI GGWCC 2 cut(s) 956, 1073
SmlI CTYRAG 3 cut(s) 767, 912, 1190
SmoI CTYRAG 3 cut(s) 767, 912, 1190
SpeI ACTAGT 1 cut(s) 347
Sse9I AATT 5 cut(s) 404, 947, 1146, 1203, 1313
SsiI CCGC 5 cut(s) 317, 330, 645, 918, 1035
SspMI CTAG 1 cut(s) 348
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 5 cut(s) 423, 583, 1084, 1135, 1184
TaqI TCGA 3 cut(s) 892, 1122, 1233
TasI AATT 5 cut(s) 404, 947, 1146, 1203, 1313
TatI WGTACW 1 cut(s) 418
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 3 cut(s) 640, 1030, 1151
Tru1I TTAA 2 cut(s) 672, 1311
Tru9I TTAA 2 cut(s) 672, 1311
TscAI CASTG 3 cut(s) 577, 1027, 1189
TseFI GTSAC 2 cut(s) 706, 799
TseI GCWGC 4 cut(s) 521, 590, 902, 1003
Tsp45I GTSAC 2 cut(s) 706, 799
TspGWI ACGGA 2 cut(s) 142, 264
TspRI CASTG 3 cut(s) 577, 1027, 1189
VpaK11BI GGWCC 2 cut(s) 956, 1073
XapI RAATTY 1 cut(s) 947
XmiI GTMKAC 1 cut(s) 630
XmnI GAANNNNTTC 2 cut(s) 947, 1135
XspI CTAG 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.