Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004891

Physical Location & Seq

Genomic Coordinates Reverse (-)

10720823 .. 10725344

Gene Structure 4522 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1139200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGCGCTCATCTGGACTTGTTGACCCTGGATGGGACCATGGCGTTGCTCAAGATGAGAGGAAGAAAAAGGTTAAATGCAATTACTGTGGAAAAATAGTTAGTGGTGGAATATACAGATTGAAGCAACATTTAGCCCGAGTTTCTGGAGAAGTTACTTATTGTGATAAAGCTCCAGACGATGTATACTTTCGTATGAAAGAAAATATGAAAGGAAGTCGTTCTCATAAGAAACCTAGGCTTTCCGAAGATATTGGCCAAGCATATCTGAATTTCCAATCCAACGATGACGAAGAAGATGTGCATGTTGGTTATAGAAGCAAAGGAAAGCAGTTGGTGGGTGAGAGGAATTTAGCTATGAAATTGACTCCTCTTCGGTCGTTAGGGTATGTTGATCCTGGGTGGGAACATGGCGTTGCTCAGGATGAGAAAAAGAAAAAGGTGAAATGCAATTATTGCGAGAAAATAGTTAGTGGTGGTATCAATCGGTTTAAACAACATTTAGCTAGAATTCCTGGAGAAGTAGCACCTTGTAAACATGCTCCTGATGATGTCTATGTTAAAATAAAGGAGAATATGAAATGGCATCGTACTGGAAGGAGACAGAGACAACCCGATTCCAAGGACATATCAAATTTTGATCCTCTGTCAGATAATGAAGGCCAAGACGATGACCAAATGGAAGCTGTTCTGCCACATATGAGCCAGGAAAGCTTCATCGATGGTGATAGGAGATTGGGCCAAAATTTGAGACATACATTCTTGTCGCTGCCTCCCAGCACTGGTTCTGAACCATTATTTAAAAGATCAAGGCTCGATTCTCTGTTCTTGACATCTCCTAAGAGTTTTCCACCACAATCTTACAAGCAAGCAAGGGTCAGAACAATGTCAAATAAAATTTCCCACAAGGAAGTTATTTCCGGAATTTGCAAATTCTTTTACCATGCAGGAGTTCCTATACAAGCAGCAGACTCCCTATACTTTCATAAGATGCTGGAATTGGTTGGCCAATATGGGCAGGGTATGGTTGCACCTCCAAGCCAAATAATAACCGGTCGGTTTCTACAAGAGGAAATTGCAACCATTAGAAACTACCTGGCTGAGTATAAGGCATCATGGGCAACCACTGGCTGTTCTATTATGGCCGACAGTTGGAGAGACACAGAGGGTAGAATATTGATAAACTTGTTGGCTTCCGGTCCAAATGGTGTGTACTTTGTTTCTTCAGTTGATGCCACTGAAATTGTTGAAGATGCTTTGAATTTGTTTATGTTGCTGGACAAAGTGGTTGAAGAGATGGGGGAGGAAAATGTAGTTCAGGTAATCACTCCAAATACTCCTAGTTATAAGGCTGCTGGAAAGATGCTTGAGGAGAAGAGAAAGAATTTATTCTGGACCCCATGCGCCACCAGTTGTATCGATCAAACGCTTGAAGATTTTTTGAGGATAAGATGCGTAGCGGAGTGCATGGAGAAGGGCCAAAAAGTTACAAAGCTCATTTACAACCAAGTTTGGTTGTTAAATTTTATGAAGAGTGATTTCACACAGGGGAAGGAACTTTTGAGATCGTCTATCACCCGGTTTGCTTCTAGCTTTGCTACCTTACAAAGCTTGCTGGACCACAAGACTGGTCTTAAAAGAATGTTTCAATCAAACAAATGGATTTCATCTCAGTGCTCCAAATCATTTGAGGGAAAAGAAGTGGAAAATATTGTCTTAAATGCTGCATTTTGGAAGAAGTTACAGTTTGTTAGGAATTCAGTGGACCCGATAATGCAAATTCTTCAAAAGGTTGAGAATGGTGACCACTTGTCAATGTCATCTATATATAATGACATGTACAGGGCAAAGATTGCAATAAAAACCATTCATGTCCGTAAATATGAACCATTTTGGAGCGTTATAGAGAGTCATTGGAACTCACTGTTCTACCACCCTGTATACGTAGCTGCTTACTACTTAAATCCATCATACAGATATCGACCTGATTTTACGGCGCATACTGAGGTGATGCGTGGACTTAACGAATGCATTGTTCGGCTGGAGCCAGACCATTCAAATAGGATTTCTGCATCTATGCAGATTTCTGATTACAATTCTGCTAAAGCTGATTTTGGAACTGAATTGGCAATCAGTACAAGAACAGAGCTTGATCCAGCTGCATGGTGGCAACAACATGGGATAAGTTGCTTAGAGCTGCAGCGTATAGCTGTGCGTATATTGAGTCAGACATGCTCTTCTTTTGGGTGTGAGCATAACTGGAGTATATACGATCAATTGTACAATCCGAGAAGTAATCGTTTGGCCCAAAAAAGGTTAAACGACCTCATCTACGTTCATTACAATTTGCGTCTTAGAGAACAAAAATTACGAAGAAGGCCGGAAAATTCAACCTCCCTTGACAATGTGCTGTTAGAGCGGTTGCTAGATGATTGGATTGTAGATGCTGCGGAAAATGACTTGCTAGACAATGAGGAAGTCCTTTACAACGAAATCGAACAGGTTGATGAATACGAAAATGATCTCGTTGATTACGAATGTGGAATTGCAAACGGAGAGACTAGGAACGGATCCATTGAGCTGGTAACCTTGGCTGATGCAGATGTCAATCCGGCCAATGCTGGCGTTGCCACCGATGATGATGACGAGGAGGAGGAGGGGGCGGAAGGTGAGATGAACTTTTTCCATGATGACTTGAGTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

901

Amino Acids

102.96

Weight (kDa)

5.91

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-BED	PF02892	11 - 46	2.2e-06	BED zinc finger
zf-BED	PF02892	134 - 169	8.1e-07	BED zinc finger
DUF659	PF04937	346 - 495	2.5e-55	Domain of unknown function (DUF659)
Dimer_Tnp_hAT	PF05699	712 - 782	2.9e-12	hAT family C-terminal dimerisation region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004891)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17450 AT3G17450
fragaria_vesca	FvH4_4g21491 FvH4_4g21491 FvH4_4g21491 FvH4_4g21491 FvH4_4g21491 FvH4_4g21491 FvH4_4g21491 FvH4_4g21491 FvH4_4g21491 FvH4_4g21491
malus_domestica	MD13G1139200.v1.1 MD16G1136600.v1.1
prunus_persica	Prupe.1G105600_v2.0.a1 Prupe.1G105600_v2.0.a1 Prupe.1G105600_v2.0.a1
pyrus_communis	pycom13g12110 pycom16g12020 pycom16g12030 pycom16g12040
rosa_chinensis	RchiOBHm_Chr4g0427461
rosa_laevigata	RLG00000007243
rosa_roxburghii	Rroxscaffold_5G00369110
rosa_rugosa	Rorug04G0208700
rosa_samantha	Rh4AG274500 Rh4BG280200 Rh4CG295200 Rh4DG277700
rosa_wichuraiana	Rw4G023780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1344
AccBSI	CCGCTC	1 cut(s)	2416
AccI	GTMKAC	2 cut(s)	183, 1938
AccIII	TCCGGA	1 cut(s)	917
AciI	CCGC	4 cut(s)	1457, 2416, 2447, 2660
AclWI	GGATC	5 cut(s)	386, 632, 2144, 2562, 2575
AcoI	YGGCCR	4 cut(s)	253, 1003, 1140, 2610
AcuI	CTGAAG	1 cut(s)	1206
AfaI	GTAC	5 cut(s)	589, 1211, 1838, 2134, 2279
AfiI	CCNNNNNNNGG	4 cut(s)	31, 779, 1149, 2310
AflIII	ACRYGT	1 cut(s)	1833
AgeI	ACCGGT	1 cut(s)	1049
AgsI	TTSAA	9 cut(s)	121, 1247, 1258, 1289, 1430, 1646, 1784, 2055, 2388
AhdI	GACNNNNNGTC	1 cut(s)	1807
AjnI	CCWGG	5 cut(s)	25, 394, 511, 702, 1092
Alw21I	GWGCWC	1 cut(s)	1676
Alw26I	GTCTC	5 cut(s)	592, 598, 742, 1149, 2549
AlwI	GGATC	5 cut(s)	386, 632, 2144, 2562, 2575
Ama87I	CYCGRG	1 cut(s)	135
Aor13HI	TCCGGA	1 cut(s)	917
AoxI	GGCC	9 cut(s)	253, 658, 736, 1003, 1140, 1474, 2301, 2375, 2610
ApeKI	GCWGC	9 cut(s)	766, 962, 1349, 1721, 1946, 2156, 2194, 2197, 2444
AsiGI	ACCGGT	1 cut(s)	1049
Asp700I	GAANNNNTTC	4 cut(s)	217, 684, 1385, 2678
AspA2I	CCTAGG	1 cut(s)	233
AspLEI	GCGC	3 cut(s)	6, 1403, 1996
AspS9I	GGNCC	8 cut(s)	34, 736, 1196, 1392, 1474, 1615, 1762, 2302
AsuC2I	CCSGG	1 cut(s)	1576
AsuHPI	GGTGA	7 cut(s)	350, 451, 734, 1564, 1811, 2017, 2678
AvaI	CYCGRG	1 cut(s)	135
AvaII	GGWCC	5 cut(s)	34, 1196, 1392, 1615, 1762
AvrII	CCTAGG	1 cut(s)	233
BalI	TGGCCA	2 cut(s)	255, 1005
BamHI	GGATCC	1 cut(s)	2567
Bbv12I	GWGCWC	1 cut(s)	1676
BbvI	GCAGC	9 cut(s)	753, 974, 1336, 1708, 1933, 2143, 2181, 2209, 2431
BccI	CCATC	4 cut(s)	24, 713, 1288, 1972
BceAI	ACGGC	1 cut(s)	2007
BciT130I	CCWGG	5 cut(s)	27, 396, 513, 704, 1094
BcnI	CCSGG	1 cut(s)	1576
BcoDI	GTCTC	5 cut(s)	592, 598, 742, 1149, 2549
BfaI	CTAG	7 cut(s)	234, 504, 1338, 1587, 2423, 2462, 2559
BfmI	CTRYAG	1 cut(s)	2195
BisI	GCNGC	9 cut(s)	767, 963, 1350, 1722, 1947, 2157, 2195, 2198, 2445
BlnI	CCTAGG	1 cut(s)	233
BlsI	GCNGC	9 cut(s)	768, 964, 1351, 1723, 1948, 2158, 2196, 2199, 2446
Bme1390I	CCNGG	6 cut(s)	27, 396, 513, 704, 1094, 1576
Bme18I	GGWCC	5 cut(s)	34, 1196, 1392, 1615, 1762
BmeRI	GACNNNNNGTC	1 cut(s)	1807
BmeT110I	CYCGRG	1 cut(s)	135
BmgT120I	GGNCC	8 cut(s)	34, 736, 1196, 1392, 1474, 1615, 1762, 2302
BmiI	GGNNCC	5 cut(s)	35, 1394, 1764, 2043, 2569
BmrFI	CCNGG	6 cut(s)	27, 396, 513, 704, 1094, 1576
BpmI	CTGGAG	5 cut(s)	156, 165, 534, 2060, 2278
Bpu10I	CCTNAGC	1 cut(s)	417
BpuEI	CTTGAG	2 cut(s)	33, 1385
BpuMI	CCSGG	1 cut(s)	1576
Bsa29I	ATCGAT	2 cut(s)	717, 1416
BsaAI	YACGTR	1 cut(s)	1942
BsaBI	GATNNNNATC	1 cut(s)	2604
BsaJI	CCNNGG	6 cut(s)	25, 37, 233, 395, 618, 2586
BsaWI	WCCGGW	3 cut(s)	917, 1049, 1193
Bsc4I	CCNNNNNNNGG	4 cut(s)	31, 779, 1149, 2310
Bse118I	RCCGGY	1 cut(s)	1049
Bse1I	ACTGG	6 cut(s)	595, 784, 1129, 1407, 1630, 2261
Bse8I	GATNNNNATC	1 cut(s)	2604
BseAI	TCCGGA	1 cut(s)	917
BseBI	CCWGG	5 cut(s)	27, 396, 513, 704, 1094
BseCI	ATCGAT	2 cut(s)	717, 1416
BseDI	CCNNGG	6 cut(s)	25, 37, 233, 395, 618, 2586
BseGI	GGATG	2 cut(s)	35, 427
BseJI	GATNNNNATC	1 cut(s)	2604
BseLI	CCNNNNNNNGG	4 cut(s)	31, 779, 1149, 2310
BseMII	CTCAG	4 cut(s)	431, 1089, 1682, 1992
BseNI	ACTGG	6 cut(s)	595, 784, 1129, 1407, 1630, 2261
BseRI	GAGGAG	5 cut(s)	357, 1382, 2660, 2663, 2666
BseXI	GCAGC	9 cut(s)	753, 974, 1336, 1708, 1933, 2143, 2181, 2209, 2431
BseYI	CCCAGC	1 cut(s)	773
Bsh1285I	CGRYCG	2 cut(s)	377, 1054
BshFI	GGCC	9 cut(s)	255, 660, 738, 1005, 1142, 1476, 2303, 2377, 2612
BshTI	ACCGGT	1 cut(s)	1049
BshVI	ATCGAT	2 cut(s)	717, 1416
BsiEI	CGRYCG	2 cut(s)	377, 1054
BsiHKAI	GWGCWC	1 cut(s)	1676
BsiHKCI	CYCGRG	1 cut(s)	135
BsiSI	CCGG	6 cut(s)	918, 1050, 1194, 1576, 2378, 2609
BslFI	GGGAC	1 cut(s)	47
BslI	CCNNNNNNNGG	4 cut(s)	31, 779, 1149, 2310
BsmAI	GTCTC	5 cut(s)	592, 598, 742, 1149, 2549
BsmFI	GGGAC	1 cut(s)	47
BsmI	GAATGC	1 cut(s)	2030
BsnI	GGCC	9 cut(s)	255, 660, 738, 1005, 1142, 1476, 2303, 2377, 2612
BsoBI	CYCGRG	1 cut(s)	135
Bsp1286I	GDGCHC	1 cut(s)	1676
Bsp13I	TCCGGA	1 cut(s)	917
Bsp1407I	TGTACA	2 cut(s)	1836, 2277
Bsp143I	GATC	9 cut(s)	391, 637, 803, 1417, 1562, 2149, 2269, 2518, 2567
Bsp19I	CCATGG	1 cut(s)	37
BspACI	CCGC	4 cut(s)	1457, 2416, 2447, 2660
BspANI	GGCC	9 cut(s)	255, 660, 738, 1005, 1142, 1476, 2303, 2377, 2612
BspCNI	CTCAG	4 cut(s)	430, 1090, 1681, 1993
BspDI	ATCGAT	2 cut(s)	717, 1416
BspEI	TCCGGA	1 cut(s)	917
BspLI	GGNNCC	5 cut(s)	35, 1394, 1764, 2043, 2569
BspMAI	CTGCAG	1 cut(s)	2199
BspPI	GGATC	5 cut(s)	386, 632, 2144, 2562, 2575
BspQI	GCTCTTC	1 cut(s)	2239
BsrBI	CCGCTC	1 cut(s)	2416
BsrFI	RCCGGY	1 cut(s)	1049
BsrGI	TGTACA	2 cut(s)	1836, 2277
BsrI	ACTGG	6 cut(s)	595, 784, 1129, 1407, 1630, 2261
BssAI	RCCGGY	1 cut(s)	1049
BssECI	CCNNGG	6 cut(s)	25, 37, 233, 395, 618, 2586
BssMI	GATC	9 cut(s)	391, 637, 803, 1417, 1562, 2149, 2269, 2518, 2567
BssNAI	GTATAC	2 cut(s)	184, 1939
BssT1I	CCWWGG	4 cut(s)	37, 233, 618, 2586
Bst1107I	GTATAC	2 cut(s)	184, 1939
Bst2UI	CCWGG	5 cut(s)	27, 396, 513, 704, 1094
Bst4CI	ACNGT	4 cut(s)	86, 1148, 1743, 1923
Bst6I	CTCTTC	5 cut(s)	375, 1284, 1367, 1523, 2239
BstAPI	GCANNNNNTGC	2 cut(s)	453, 1850
BstAUI	TGTACA	2 cut(s)	1836, 2277
BstBAI	YACGTR	1 cut(s)	1942
BstC8I	GCNNGC	3 cut(s)	867, 1610, 2620
BstDEI	CTNAG	7 cut(s)	417, 837, 1098, 1668, 2001, 2188, 2351
BstDSI	CCRYGG	1 cut(s)	37
BstEII	GGTNACC	2 cut(s)	1799, 2581
BstF5I	GGATG	2 cut(s)	35, 427
BstHHI	GCGC	3 cut(s)	6, 1403, 1996
BstKTI	GATC	9 cut(s)	394, 640, 806, 1420, 1565, 2152, 2272, 2521, 2570
BstMAI	GTCTC	5 cut(s)	592, 598, 742, 1149, 2549
BstMBI	GATC	9 cut(s)	391, 637, 803, 1417, 1562, 2149, 2269, 2518, 2567
BstMCI	CGRYCG	2 cut(s)	377, 1054
BstMWI	GCNNNNNNNGC	6 cut(s)	453, 708, 1115, 1850, 2413, 2624
BstNI	CCWGG	5 cut(s)	27, 396, 513, 704, 1094
BstNSI	RCATGY	4 cut(s)	305, 539, 1837, 2232
BstPI	GGTNACC	2 cut(s)	1799, 2581
BstSCI	CCNGG	6 cut(s)	25, 394, 511, 702, 1092, 1574
BstSFI	CTRYAG	1 cut(s)	2195
BstSNI	TACGTA	1 cut(s)	1942
BstV1I	GCAGC	9 cut(s)	753, 974, 1336, 1708, 1933, 2143, 2181, 2209, 2431
BstX2I	RGATCY	1 cut(s)	2567
BstXI	CCANNNNNNTGG	3 cut(s)	1625, 2160, 2578
BstYI	RGATCY	1 cut(s)	2567
BstZ17I	GTATAC	2 cut(s)	184, 1939
Bsu15I	ATCGAT	2 cut(s)	717, 1416
BsuRI	GGCC	9 cut(s)	255, 660, 738, 1005, 1142, 1476, 2303, 2377, 2612
BsuTUI	ATCGAT	2 cut(s)	717, 1416
BtgI	CCRYGG	1 cut(s)	37
BtsCI	GGATG	2 cut(s)	35, 427
BtsIMutI	CAGTG	6 cut(s)	777, 1122, 1233, 1676, 1764, 1919
Cac8I	GCNNGC	3 cut(s)	867, 1610, 2620
CfoI	GCGC	3 cut(s)	6, 1403, 1996
Cfr10I	RCCGGY	1 cut(s)	1049
Cfr13I	GGNCC	8 cut(s)	34, 736, 1196, 1392, 1474, 1615, 1762, 2302
ClaI	ATCGAT	2 cut(s)	717, 1416
CseI	GACGC	1 cut(s)	2336
Csp6I	GTAC	5 cut(s)	588, 1210, 1837, 2133, 2278
CspAI	ACCGGT	1 cut(s)	1049
CspCI	CAANNNNNGTGG	4 cut(s)	67, 102, 1222, 1257
CviQI	GTAC	5 cut(s)	588, 1210, 1837, 2133, 2278
DdeI	CTNAG	7 cut(s)	417, 837, 1098, 1668, 2001, 2188, 2351
DpnI	GATC	9 cut(s)	393, 639, 805, 1419, 1564, 2151, 2271, 2520, 2569
DpnII	GATC	9 cut(s)	391, 637, 803, 1417, 1562, 2149, 2269, 2518, 2567
DraI	TTTAAA	2 cut(s)	490, 799
DriI	GACNNNNNGTC	1 cut(s)	1807
EaeI	YGGCCR	4 cut(s)	253, 1003, 1140, 2610
Eam1104I	CTCTTC	5 cut(s)	375, 1284, 1367, 1523, 2239
Eam1105I	GACNNNNNGTC	1 cut(s)	1807
EarI	CTCTTC	5 cut(s)	375, 1284, 1367, 1523, 2239
EciI	GGCGGA	1 cut(s)	2675
Eco105I	TACGTA	1 cut(s)	1942
Eco130I	CCWWGG	4 cut(s)	37, 233, 618, 2586
Eco32I	GATATC	1 cut(s)	1976
Eco47I	GGWCC	5 cut(s)	34, 1196, 1392, 1615, 1762
Eco57I	CTGAAG	1 cut(s)	1206
Eco88I	CYCGRG	1 cut(s)	135
Eco91I	GGTNACC	2 cut(s)	1799, 2581
EcoO65I	GGTNACC	2 cut(s)	1799, 2581
EcoRI	GAATTC	2 cut(s)	507, 1753
EcoRII	CCWGG	5 cut(s)	25, 394, 511, 702, 1092
EcoRV	GATATC	1 cut(s)	1976
EcoT14I	CCWWGG	4 cut(s)	37, 233, 618, 2586
EcoT22I	ATGCAT	1 cut(s)	2030
ErhI	CCWWGG	4 cut(s)	37, 233, 618, 2586
FaqI	GGGAC	1 cut(s)	47
FauNDI	CATATG	1 cut(s)	696
FblI	GTMKAC	2 cut(s)	183, 1938
Fnu4HI	GCNGC	9 cut(s)	767, 963, 1350, 1722, 1947, 2157, 2195, 2198, 2445
FokI	GGATG	2 cut(s)	42, 434
Fsp4HI	GCNGC	9 cut(s)	767, 963, 1350, 1722, 1947, 2157, 2195, 2198, 2445
FspBI	CTAG	7 cut(s)	234, 504, 1338, 1587, 2423, 2462, 2559
GlaI	GCGC	3 cut(s)	5, 1402, 1995
GluI	GCNGC	9 cut(s)	767, 963, 1350, 1722, 1947, 2157, 2195, 2198, 2445
GsaI	CCCAGC	1 cut(s)	777
GsuI	CTGGAG	5 cut(s)	156, 165, 534, 2060, 2278
HaeIII	GGCC	9 cut(s)	255, 660, 738, 1005, 1142, 1476, 2303, 2377, 2612
HapII	CCGG	6 cut(s)	918, 1050, 1194, 1576, 2378, 2609
HgaI	GACGC	1 cut(s)	2336
HhaI	GCGC	3 cut(s)	6, 1403, 1996
Hin6I	GCGC	3 cut(s)	4, 1401, 1994
HinP1I	GCGC	3 cut(s)	4, 1401, 1994
HincII	GTYRAC	1 cut(s)	22
HindII	GTYRAC	1 cut(s)	22
HindIII	AAGCTT	2 cut(s)	709, 1606
HinfI	GANTC	6 cut(s)	364, 614, 815, 968, 1906, 2221
HpaII	CCGG	6 cut(s)	918, 1050, 1194, 1576, 2378, 2609
HphI	GGTGA	7 cut(s)	350, 451, 734, 1564, 1811, 2017, 2678
Hpy166II	GTNNAC	7 cut(s)	22, 184, 533, 1210, 1762, 1939, 2015
Hpy188I	TCNGA	8 cut(s)	244, 267, 649, 787, 878, 2086, 2226, 2286
Hpy188III	TCNNGA	9 cut(s)	12, 50, 144, 173, 419, 542, 826, 918, 1390
Hpy8I	GTNNAC	7 cut(s)	22, 184, 533, 1210, 1762, 1939, 2015
HpyAV	CCTTC	6 cut(s)	588, 650, 1465, 1543, 2367, 2657
HpyCH4III	ACNGT	4 cut(s)	86, 1148, 1743, 1923
HpyCH4IV	ACGT	2 cut(s)	1941, 2331
HpyF10VI	GCNNNNNNNGC	6 cut(s)	453, 708, 1115, 1850, 2413, 2624
HpyF3I	CTNAG	7 cut(s)	417, 837, 1098, 1668, 2001, 2188, 2351
HpySE526I	ACGT	2 cut(s)	1941, 2331
HspAI	GCGC	3 cut(s)	4, 1401, 1994
Kpn2I	TCCGGA	1 cut(s)	917
Kzo9I	GATC	9 cut(s)	391, 637, 803, 1417, 1562, 2149, 2269, 2518, 2567
LguI	GCTCTTC	1 cut(s)	2239
LmnI	GCTCC	5 cut(s)	175, 544, 1679, 1893, 2041
Lsp1109I	GCAGC	9 cut(s)	753, 974, 1336, 1708, 1933, 2143, 2181, 2209, 2431
MaeI	CTAG	7 cut(s)	234, 504, 1338, 1587, 2423, 2462, 2559
MaeII	ACGT	2 cut(s)	1941, 2331
MaeIII	GTNAC	5 cut(s)	151, 1483, 1737, 1799, 2581
MalI	GATC	9 cut(s)	393, 639, 805, 1419, 1564, 2151, 2271, 2520, 2569
MbiI	CCGCTC	1 cut(s)	2416
MboI	GATC	9 cut(s)	391, 637, 803, 1417, 1562, 2149, 2269, 2518, 2567
MfeI	CAATTG	1 cut(s)	2273
MflI	RGATCY	1 cut(s)	2567
MhlI	GDGCHC	1 cut(s)	1676
MlsI	TGGCCA	2 cut(s)	255, 1005
MluNI	TGGCCA	2 cut(s)	255, 1005
MlyI	GAGTC	4 cut(s)	358, 962, 1915, 2230
MmeI	TCCRAC	2 cut(s)	303, 1130
Mox20I	TGGCCA	2 cut(s)	255, 1005
Mph1103I	ATGCAT	1 cut(s)	2030
MroI	TCCGGA	1 cut(s)	917
MroXI	GAANNNNTTC	4 cut(s)	217, 684, 1385, 2678
MscI	TGGCCA	2 cut(s)	255, 1005
MslI	CAYNNNNRTG	1 cut(s)	1669
Msp20I	TGGCCA	2 cut(s)	255, 1005
MspA1I	CMGCKG	1 cut(s)	2156
MspI	CCGG	6 cut(s)	918, 1050, 1194, 1576, 2378, 2609
MspR9I	CCNGG	6 cut(s)	27, 396, 513, 704, 1094, 1576
MssI	GTTTAAAC	1 cut(s)	490
MunI	CAATTG	1 cut(s)	2273
Mva1269I	GAATGC	1 cut(s)	2030
MvaI	CCWGG	5 cut(s)	27, 396, 513, 704, 1094
MwoI	GCNNNNNNNGC	6 cut(s)	453, 708, 1115, 1850, 2413, 2624
NciI	CCSGG	1 cut(s)	1576
NcoI	CCATGG	1 cut(s)	37
NdeI	CATATG	1 cut(s)	696
NdeII	GATC	9 cut(s)	391, 637, 803, 1417, 1562, 2149, 2269, 2518, 2567
NlaIV	GGNNCC	5 cut(s)	35, 1394, 1764, 2043, 2569
NmuCI	GTSAC	1 cut(s)	1799
NsiI	ATGCAT	1 cut(s)	2030
NspI	RCATGY	4 cut(s)	305, 539, 1837, 2232
PciI	ACATGT	1 cut(s)	1833
PciSI	GCTCTTC	1 cut(s)	2239
PcsI	WCGNNNNNNNCGW	1 cut(s)	2529
PctI	GAATGC	1 cut(s)	2030
PdmI	GAANNNNTTC	4 cut(s)	217, 684, 1385, 2678
PfeI	GAWTC	2 cut(s)	614, 815
PfoI	TCCNGGA	1 cut(s)	511
PinAI	ACCGGT	1 cut(s)	1049
PkrI	GCNGC	9 cut(s)	768, 964, 1351, 1723, 1948, 2158, 2196, 2199, 2446
PleI	GAGTC	4 cut(s)	358, 962, 1914, 2229
PmeI	GTTTAAAC	1 cut(s)	490
PpsI	GAGTC	4 cut(s)	358, 962, 1914, 2229
Ppu21I	YACGTR	1 cut(s)	1942
PscI	ACATGT	1 cut(s)	1833
PsiI	TTATAA	1 cut(s)	1344
Psp6I	CCWGG	5 cut(s)	25, 394, 511, 702, 1092
PspEI	GGTNACC	2 cut(s)	1799, 2581
PspFI	CCCAGC	1 cut(s)	773
PspGI	CCWGG	5 cut(s)	25, 394, 511, 702, 1092
PspN4I	GGNNCC	5 cut(s)	35, 1394, 1764, 2043, 2569
PspPI	GGNCC	8 cut(s)	34, 736, 1196, 1392, 1474, 1615, 1762, 2302
PsrI	GAACNNNNNNTAC	2 cut(s)	2349, 2381
PstI	CTGCAG	1 cut(s)	2199
PsuI	RGATCY	1 cut(s)	2567
PvuII	CAGCTG	1 cut(s)	2156
RsaI	GTAC	5 cut(s)	589, 1211, 1838, 2134, 2279
RsaNI	GTAC	5 cut(s)	588, 1210, 1837, 2133, 2278
RseI	CAYNNNNRTG	1 cut(s)	1669
SapI	GCTCTTC	1 cut(s)	2239
SatI	GCNGC	9 cut(s)	767, 963, 1350, 1722, 1947, 2157, 2195, 2198, 2445
Sau3AI	GATC	9 cut(s)	391, 637, 803, 1417, 1562, 2149, 2269, 2518, 2567
Sau96I	GGNCC	8 cut(s)	34, 736, 1196, 1392, 1474, 1615, 1762, 2302
SchI	GAGTC	4 cut(s)	358, 962, 1915, 2230
ScrFI	CCNGG	6 cut(s)	27, 396, 513, 704, 1094, 1576
SduI	GDGCHC	1 cut(s)	1676
SfcI	CTRYAG	1 cut(s)	2195
SinI	GGWCC	5 cut(s)	34, 1196, 1392, 1615, 1762
SmiMI	CAYNNNNRTG	1 cut(s)	1669
SmlI	CTYRAG	3 cut(s)	48, 1364, 2692
SmoI	CTYRAG	3 cut(s)	48, 1364, 2692
SnaBI	TACGTA	1 cut(s)	1942
SsiI	CCGC	4 cut(s)	1457, 2416, 2447, 2660
SspI	AATATT	2 cut(s)	1173, 1708
SspMI	CTAG	7 cut(s)	234, 504, 1338, 1587, 2423, 2462, 2559
StyD4I	CCNGG	6 cut(s)	25, 394, 511, 702, 1092, 1574
StyI	CCWWGG	4 cut(s)	37, 233, 618, 2586
TaaI	ACNGT	4 cut(s)	86, 1148, 1743, 1923
TaiI	ACGT	2 cut(s)	1944, 2334
TaqI	TCGA	5 cut(s)	717, 813, 1416, 1978, 2493
TaqII	GACCGA	1 cut(s)	363
TatI	WGTACW	4 cut(s)	1209, 1836, 2132, 2277
TfiI	GAWTC	2 cut(s)	614, 815
TscAI	CASTG	6 cut(s)	784, 1129, 1240, 1676, 1764, 1926
TseFI	GTSAC	1 cut(s)	1799
TseI	GCWGC	9 cut(s)	766, 962, 1349, 1721, 1946, 2156, 2194, 2197, 2444
Tsp45I	GTSAC	1 cut(s)	1799
TspGWI	ACGGA	3 cut(s)	1862, 2565, 2580
TspRI	CASTG	6 cut(s)	784, 1129, 1240, 1676, 1764, 1926
VpaK11BI	GGWCC	5 cut(s)	34, 1196, 1392, 1615, 1762
XceI	RCATGY	4 cut(s)	305, 539, 1837, 2232
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2160
XmaJI	CCTAGG	1 cut(s)	233
XmiI	GTMKAC	2 cut(s)	183, 1938
XmnI	GAANNNNTTC	4 cut(s)	217, 684, 1385, 2678
XspI	CTAG	7 cut(s)	234, 504, 1338, 1587, 2423, 2462, 2559
Zsp2I	ATGCAT	1 cut(s)	2030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.