Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0013844

Physical Location & Seq

Genomic Coordinates Reverse (-)

11181740 .. 11184927

Gene Structure 3188 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1143800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGCCCGCAAGAAGATCAGAGAGTACGACTCCAAGAGGCTGCTCAAACAGCACCTGAAACGACTCGCCGGAATCGACCTCCAGATCAACTCAGCTCTGGTGACCGAGTCGACGGACTTCTCCGAGTTGTCGAACGAGCATACATGGCTGTCGTCTACGCGCCTCGTGGTGAAGCCCGACATGCTGTTCGGGAAGCGCGGGAAGAGCGGCCTCGTCGCCCTGAACTTGGACATGGCTCAGGTCGCCGAGTTCGTCAAGCAGCGACTCGGTAAGGAGGTTGAGATGGGAGGATGCAAGGCGCCGATTACGACGTTTATAGTGGAGCCGTTCATGCCCCATGATCAAGAATTTTACCTCTCAATTGTGTCCGAAAGGCTCGGATCAACCGTGAGCTTTTCGGAGTGTGGAGGGATCGAAATTGAAGAGAATTGGGACAAGGTTAAGACTATATTTCTTCCGACCGAGAAGCCTATGACGAATGATGCTTGTGCTCCACTGATTGCCACTCTTCCATTGGAGGTCAGAGGCAAAATTGGAGATTTCATAAAGGGCGTATTTGCTGTATTCCAGGATTTGGATTTTAGTTTCCTGGAAATGAACCCGTTTACCTTGGTTGATGGTGAACCTTATCCACTAGATATGAGGGGGGAGCTAGATGACACTGCTGCCTTCAAGAACTTCAATAAATGGGGCAACGTGGAGTTTCCATTGCCTTTCGGCAGAGTCTTAAGTCCTACAGAAACATTTATTCATTCTCTGGATGAAAAGACAAGTGCATCCTTAAAATTCACTGTGTTGAACCCAAAGGGACGCATCTGGACCATGGTAGCTGGAGGCGGTGCGAGTGTTATTTATGCTGATACTGTTGGAGATTTGGGTTATGCTTCAGAACTTGGAAATTATGCAGAGTATAGTGGAGCTCCAAATGAGGGAGAGGTGTTGCAGTATGCAAGAGTTGTGATCGATTGTGCAACGGCTGATCCAGATGGTCGTAAAAGAGCACTTATAATTGGAGGTGGCATAGCTAATTTCACAGACGTAGCTGCTACATTCAATGGAATTATTAGAGCCCTCAAGGAGAAGGAATCCAAGCTAAAAGCAGCAAGAATGCATATTTTTGTGAGAAGGGGTGGTCCAAACTATCAAACTGGTTTGGCAAAGATGCGCGCATTGGGAGAAGAACTTGGAGTTCCGCTTGAGGTTTATGGCCCTGAGGCAACAATGACGGGAATTTGCAAGCAAGCAATTGAGTGCATCATGGGTGAAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003878 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006629 GO:0006631 GO:0006633 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009346 GO:0009987 GO:0016053 GO:0016740 GO:0016746 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0032991 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046390 GO:0046394 GO:0046483 GO:0046912 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00020 ko00720 ko01100 ko01110 ko01120 ko01130 map00020 map00720 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

ATP-grasp_2 Citrate_bind

Protein Analysis

424

Amino Acids

46.52

Weight (kDa)

5.43

Isoelectric Point (pI)

35.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Citrate_synth_N	PF24948	2 - 230	4.2e-73	ATP-citrate synthase ATP-grasp domain
ATP-grasp_2	PF08442	6 - 203	3.1e-17	ATP-grasp domain
Citrate_bind	PF16114	241 - 417	1.8e-84	ATP citrate lyase citrate-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013844)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09430
fragaria_vesca	FvH4_4g21220
malus_domestica	MD13G1143800.v1.1
prunus_persica	Prupe.1G108300_v2.0.a1 Prupe.6G110400_v2.0.a1
pyrus_communis	pycom13g12470
rosa_chinensis	RchiOBHm_Chr4g0426831
rosa_laevigata	RLG00000007274
rosa_multiflora	Rmu_sc0000953.1_g000013 Rmu_sc0004341.1_g000006
rosa_roxburghii	Rroxscaffold_5G00368800
rosa_rugosa	Rorug04G0215200
rosa_samantha	Rh4AG270200 Rh4BG276500 Rh4CG291300 Rh4DG273800
rosa_wichuraiana	Rw4G023450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1007
AccB1I	GGYRCC	1 cut(s)	298
AccB7I	CCANNNNNTGG	1 cut(s)	574
AccBSI	CCGCTC	1 cut(s)	207
AccI	GTMKAC	2 cut(s)	110, 155
AccII	CGCG	3 cut(s)	160, 198, 1167
AciI	CCGC	5 cut(s)	7, 198, 207, 837, 1193
AclWI	GGATC	3 cut(s)	388, 419, 974
AcsI	RAATTY	3 cut(s)	347, 785, 1230
AcuI	CTGAAG	1 cut(s)	870
AcyI	GRCGYC	1 cut(s)	299
AfaI	GTAC	1 cut(s)	26
AfiI	CCNNNNNNNGG	3 cut(s)	226, 574, 929
AflII	CTTAAG	1 cut(s)	727
AgsI	TTSAA	5 cut(s)	422, 673, 682, 799, 1054
AjnI	CCWGG	2 cut(s)	567, 588
AluBI	AGCT	9 cut(s)	95, 393, 652, 830, 920, 1025, 1043, 1093, 1268
AluI	AGCT	9 cut(s)	95, 393, 652, 830, 920, 1025, 1043, 1093, 1268
Alw21I	GWGCWC	3 cut(s)	493, 922, 1003
AlwI	GGATC	3 cut(s)	388, 419, 974
AlwNI	CAGNNNCTG	1 cut(s)	55
AoxI	GGCC	3 cut(s)	3, 208, 1207
ApeKI	GCWGC	5 cut(s)	40, 259, 665, 1043, 1100
ApoI	RAATTY	3 cut(s)	347, 785, 1230
ArsI	GACNNNNNNTTYG	2 cut(s)	170, 202
AspLEI	GCGC	5 cut(s)	162, 198, 301, 1167, 1169
AspS9I	GGNCC	4 cut(s)	4, 819, 1133, 1208
AsuHPI	GGTGA	3 cut(s)	112, 181, 632
AvaII	GGWCC	2 cut(s)	819, 1133
AxyI	CCTNAGG	1 cut(s)	1212
BanI	GGYRCC	1 cut(s)	298
BanII	GRGCYC	2 cut(s)	922, 1072
BauI	CACGAG	1 cut(s)	164
Bbv12I	GWGCWC	3 cut(s)	493, 922, 1003
BbvI	GCAGC	5 cut(s)	27, 271, 652, 1030, 1112
BccI	CCATC	3 cut(s)	277, 611, 980
BceAI	ACGGC	2 cut(s)	310, 990
BciT130I	CCWGG	2 cut(s)	569, 590
BclI	TGATCA	1 cut(s)	340
BfaI	CTAG	2 cut(s)	635, 653
BfmI	CTRYAG	1 cut(s)	735
BfoI	RGCGCY	1 cut(s)	302
BfrI	CTTAAG	1 cut(s)	727
BisI	GCNGC	6 cut(s)	41, 208, 260, 666, 1044, 1101
BlsI	GCNGC	6 cut(s)	42, 209, 261, 667, 1045, 1102
Bme1390I	CCNGG	2 cut(s)	569, 590
Bme18I	GGWCC	2 cut(s)	819, 1133
BmgT120I	GGNCC	4 cut(s)	4, 819, 1133, 1208
BmiI	GGNNCC	2 cut(s)	300, 324
BmrFI	CCNGG	2 cut(s)	569, 590
BmsI	GCATC	6 cut(s)	281, 472, 785, 822, 1152, 1263
BplI	GAGNNNNNCTC	2 cut(s)	14, 46
BpmI	CTGGAG	2 cut(s)	65, 852
Bpu10I	CCTNAGC	1 cut(s)	237
BpuEI	CTTGAG	2 cut(s)	1058, 1217
Bsa29I	ATCGAT	1 cut(s)	963
BsaHI	GRCGYC	1 cut(s)	299
BsaJI	CCNNGG	2 cut(s)	609, 822
Bsc4I	CCNNNNNNNGG	3 cut(s)	226, 574, 929
Bse1I	ACTGG	1 cut(s)	1153
Bse21I	CCTNAGG	1 cut(s)	1212
Bse3DI	GCAATG	1 cut(s)	707
BseBI	CCWGG	2 cut(s)	569, 590
BseCI	ATCGAT	1 cut(s)	963
BseDI	CCNNGG	2 cut(s)	609, 822
BseGI	GGATG	3 cut(s)	296, 766, 776
BseLI	CCNNNNNNNGG	3 cut(s)	226, 574, 929
BseMI	GCAATG	1 cut(s)	707
BseMII	CTCAG	3 cut(s)	105, 251, 1203
BseNI	ACTGG	1 cut(s)	1153
BsePI	GCGCGC	1 cut(s)	1165
BseXI	GCAGC	5 cut(s)	27, 271, 652, 1030, 1112
Bsh1236I	CGCG	3 cut(s)	160, 198, 1167
Bsh1285I	CGRYCG	1 cut(s)	462
BshFI	GGCC	3 cut(s)	5, 210, 1209
BshNI	GGYRCC	1 cut(s)	298
BshVI	ATCGAT	1 cut(s)	963
BsiEI	CGRYCG	1 cut(s)	462
BsiHKAI	GWGCWC	3 cut(s)	493, 922, 1003
BsiSI	CCGG	1 cut(s)	69
BslFI	GGGAC	2 cut(s)	446, 822
BslI	CCNNNNNNNGG	3 cut(s)	226, 574, 929
BsmFI	GGGAC	2 cut(s)	446, 822
BsmI	GAATGC	1 cut(s)	1113
BsnI	GGCC	3 cut(s)	5, 210, 1209
Bsp1286I	GDGCHC	4 cut(s)	493, 922, 1003, 1072
Bsp143I	GATC	7 cut(s)	15, 84, 340, 380, 411, 960, 979
Bsp19I	CCATGG	1 cut(s)	822
BspACI	CCGC	5 cut(s)	7, 198, 207, 837, 1193
BspANI	GGCC	3 cut(s)	5, 210, 1209
BspCNI	CTCAG	3 cut(s)	104, 250, 1204
BspDI	ATCGAT	1 cut(s)	963
BspFNI	CGCG	3 cut(s)	160, 198, 1167
BspLI	GGNNCC	2 cut(s)	300, 324
BspPI	GGATC	3 cut(s)	388, 419, 974
BspQI	GCTCTTC	1 cut(s)	197
BspT107I	GGYRCC	1 cut(s)	298
BspTI	CTTAAG	1 cut(s)	727
BsrBI	CCGCTC	1 cut(s)	207
BsrDI	GCAATG	1 cut(s)	707
BsrI	ACTGG	1 cut(s)	1153
BssECI	CCNNGG	2 cut(s)	609, 822
BssHII	GCGCGC	1 cut(s)	1165
BssMI	GATC	7 cut(s)	15, 84, 340, 380, 411, 960, 979
BssNI	GRCGYC	1 cut(s)	299
BssSI	CACGAG	1 cut(s)	164
BssT1I	CCWWGG	2 cut(s)	609, 822
Bst2BI	CACGAG	1 cut(s)	164
Bst2UI	CCWGG	2 cut(s)	569, 590
Bst4CI	ACNGT	3 cut(s)	388, 793, 865
Bst6I	CTCTTC	3 cut(s)	197, 417, 513
BstACI	GRCGYC	1 cut(s)	299
BstAFI	CTTAAG	1 cut(s)	727
BstC8I	GCNNGC	4 cut(s)	7, 1167, 1238, 1242
BstDEI	CTNAG	3 cut(s)	91, 237, 1212
BstDSI	CCRYGG	1 cut(s)	822
BstEII	GGTNACC	1 cut(s)	100
BstF5I	GGATG	3 cut(s)	296, 766, 776
BstFNI	CGCG	3 cut(s)	160, 198, 1167
BstH2I	RGCGCY	1 cut(s)	302
BstHHI	GCGC	5 cut(s)	162, 198, 301, 1167, 1169
BstKTI	GATC	7 cut(s)	18, 87, 343, 383, 414, 963, 982
BstMBI	GATC	7 cut(s)	15, 84, 340, 380, 411, 960, 979
BstMCI	CGRYCG	1 cut(s)	462
BstMWI	GCNNNNNNNGC	6 cut(s)	49, 145, 181, 204, 242, 331
BstNI	CCWGG	2 cut(s)	569, 590
BstNSI	RCATGY	1 cut(s)	184
BstPI	GGTNACC	1 cut(s)	100
BstSCI	CCNGG	2 cut(s)	567, 588
BstSFI	CTRYAG	1 cut(s)	735
BstUI	CGCG	3 cut(s)	160, 198, 1167
BstV1I	GCAGC	5 cut(s)	27, 271, 652, 1030, 1112
Bsu15I	ATCGAT	1 cut(s)	963
Bsu36I	CCTNAGG	1 cut(s)	1212
BsuRI	GGCC	3 cut(s)	5, 210, 1209
BsuTUI	ATCGAT	1 cut(s)	963
BtgI	CCRYGG	1 cut(s)	822
BtsCI	GGATG	3 cut(s)	296, 766, 776
BtsI	GCAGTG	1 cut(s)	660
BtsIMutI	CAGTG	3 cut(s)	494, 660, 789
Cac8I	GCNNGC	4 cut(s)	7, 1167, 1238, 1242
CaiI	CAGNNNCTG	1 cut(s)	55
CfoI	GCGC	5 cut(s)	162, 198, 301, 1167, 1169
Cfr13I	GGNCC	4 cut(s)	4, 819, 1133, 1208
ClaI	ATCGAT	1 cut(s)	963
CseI	GACGC	1 cut(s)	819
Csp6I	GTAC	1 cut(s)	25
CviAII	CATG	7 cut(s)	144, 181, 232, 331, 338, 823, 1258
CviQI	GTAC	1 cut(s)	25
DdeI	CTNAG	3 cut(s)	91, 237, 1212
DinI	GGCGCC	1 cut(s)	300
DpnI	GATC	7 cut(s)	17, 86, 342, 382, 413, 962, 981
DpnII	GATC	7 cut(s)	15, 84, 340, 380, 411, 960, 979
Eam1104I	CTCTTC	3 cut(s)	197, 417, 513
EarI	CTCTTC	3 cut(s)	197, 417, 513
Ecl136II	GAGCTC	1 cut(s)	920
Eco130I	CCWWGG	2 cut(s)	609, 822
Eco24I	GRGCYC	2 cut(s)	922, 1072
Eco47I	GGWCC	2 cut(s)	819, 1133
Eco53kI	GAGCTC	1 cut(s)	920
Eco57I	CTGAAG	1 cut(s)	870
Eco81I	CCTNAGG	1 cut(s)	1212
Eco91I	GGTNACC	1 cut(s)	100
EcoICRI	GAGCTC	1 cut(s)	920
EcoO65I	GGTNACC	1 cut(s)	100
EcoRII	CCWGG	2 cut(s)	567, 588
EcoT14I	CCWWGG	2 cut(s)	609, 822
EcoT22I	ATGCAT	1 cut(s)	1113
EcoT38I	GRGCYC	2 cut(s)	922, 1072
EgeI	GGCGCC	1 cut(s)	300
EheI	GGCGCC	1 cut(s)	300
ErhI	CCWWGG	2 cut(s)	609, 822
FaeI	CATG	7 cut(s)	147, 184, 235, 334, 341, 826, 1261
FaqI	GGGAC	2 cut(s)	446, 822
FatI	CATG	7 cut(s)	143, 180, 231, 330, 337, 822, 1257
FauI	CCCGC	2 cut(s)	14, 191
FbaI	TGATCA	1 cut(s)	340
FblI	GTMKAC	2 cut(s)	110, 155
Fnu4HI	GCNGC	6 cut(s)	41, 208, 260, 666, 1044, 1101
FokI	GGATG	3 cut(s)	303, 763, 773
FriOI	GRGCYC	2 cut(s)	922, 1072
Fsp4HI	GCNGC	6 cut(s)	41, 208, 260, 666, 1044, 1101
FspBI	CTAG	2 cut(s)	635, 653
GlaI	GCGC	5 cut(s)	161, 197, 300, 1166, 1168
GluI	GCNGC	6 cut(s)	41, 208, 260, 666, 1044, 1101
GsuI	CTGGAG	2 cut(s)	65, 852
HaeII	RGCGCY	1 cut(s)	302
HaeIII	GGCC	3 cut(s)	5, 210, 1209
HapII	CCGG	1 cut(s)	69
HgaI	GACGC	1 cut(s)	819
HhaI	GCGC	5 cut(s)	162, 198, 301, 1167, 1169
Hin1I	GRCGYC	1 cut(s)	299
Hin1II	CATG	7 cut(s)	147, 184, 235, 334, 341, 826, 1261
Hin6I	GCGC	5 cut(s)	160, 196, 299, 1165, 1167
HinP1I	GCGC	5 cut(s)	160, 196, 299, 1165, 1167
HincII	GTYRAC	1 cut(s)	111
HindII	GTYRAC	1 cut(s)	111
HindIII	AAGCTT	1 cut(s)	1266
HinfI	GANTC	7 cut(s)	29, 63, 72, 107, 264, 723, 1085
HpaII	CCGG	1 cut(s)	69
HphI	GGTGA	3 cut(s)	112, 181, 632
Hpy166II	GTNNAC	4 cut(s)	111, 156, 606, 623
Hpy188I	TCNGA	8 cut(s)	20, 124, 370, 380, 400, 459, 524, 889
Hpy188III	TCNNGA	7 cut(s)	82, 190, 344, 673, 758, 817, 983
Hpy8I	GTNNAC	4 cut(s)	111, 156, 606, 623
Hpy99I	CGWCG	3 cut(s)	115, 218, 313
HpyAV	CCTTC	3 cut(s)	679, 1075, 1119
HpyCH4III	ACNGT	3 cut(s)	388, 793, 865
HpyCH4IV	ACGT	3 cut(s)	311, 696, 1038
HpyCH4V	TGCA	9 cut(s)	294, 776, 905, 943, 950, 971, 1111, 1236, 1254
HpyF10VI	GCNNNNNNNGC	6 cut(s)	49, 145, 181, 204, 242, 331
HpyF3I	CTNAG	3 cut(s)	91, 237, 1212
HpySE526I	ACGT	3 cut(s)	311, 696, 1038
Hsp92I	GRCGYC	1 cut(s)	299
Hsp92II	CATG	7 cut(s)	147, 184, 235, 334, 341, 826, 1261
HspAI	GCGC	5 cut(s)	160, 196, 299, 1165, 1167
KasI	GGCGCC	1 cut(s)	298
Ksp22I	TGATCA	1 cut(s)	340
Kzo9I	GATC	7 cut(s)	15, 84, 340, 380, 411, 960, 979
LguI	GCTCTTC	1 cut(s)	197
LmnI	GCTCC	5 cut(s)	322, 496, 649, 917, 925
Lsp1109I	GCAGC	5 cut(s)	27, 271, 652, 1030, 1112
LweI	GCATC	6 cut(s)	281, 472, 785, 822, 1152, 1263
MaeI	CTAG	2 cut(s)	635, 653
MaeII	ACGT	3 cut(s)	311, 696, 1038
MaeIII	GTNAC	1 cut(s)	100
MalI	GATC	7 cut(s)	17, 86, 342, 382, 413, 962, 981
MbiI	CCGCTC	1 cut(s)	207
MboI	GATC	7 cut(s)	15, 84, 340, 380, 411, 960, 979
MboII	GAAGA	6 cut(s)	25, 214, 434, 446, 500, 1190
MfeI	CAATTG	2 cut(s)	360, 1245
MhlI	GDGCHC	4 cut(s)	493, 922, 1003, 1072
Mly113I	GGCGCC	1 cut(s)	299
MlyI	GAGTC	5 cut(s)	23, 57, 116, 258, 732
MmeI	TCCRAC	2 cut(s)	482, 847
Mph1103I	ATGCAT	1 cut(s)	1113
MseI	TTAA	4 cut(s)	441, 728, 782, 1270
MspCI	CTTAAG	1 cut(s)	727
MspI	CCGG	1 cut(s)	69
MspR9I	CCNGG	2 cut(s)	569, 590
MunI	CAATTG	2 cut(s)	360, 1245
Mva1269I	GAATGC	1 cut(s)	1113
MvaI	CCWGG	2 cut(s)	569, 590
MvnI	CGCG	3 cut(s)	160, 198, 1167
MwoI	GCNNNNNNNGC	6 cut(s)	49, 145, 181, 204, 242, 331
NarI	GGCGCC	1 cut(s)	299
NcoI	CCATGG	1 cut(s)	822
NdeII	GATC	7 cut(s)	15, 84, 340, 380, 411, 960, 979
NlaIII	CATG	7 cut(s)	147, 184, 235, 334, 341, 826, 1261
NlaIV	GGNNCC	2 cut(s)	300, 324
NmeAIII	GCCGAG	1 cut(s)	271
NmuCI	GTSAC	1 cut(s)	100
NsiI	ATGCAT	1 cut(s)	1113
NspI	RCATGY	1 cut(s)	184
PauI	GCGCGC	1 cut(s)	1165
PciSI	GCTCTTC	1 cut(s)	197
PcsI	WCGNNNNNNNCGW	3 cut(s)	72, 249, 384
PctI	GAATGC	1 cut(s)	1113
PfeI	GAWTC	2 cut(s)	72, 1085
PflFI	GACNNNGTC	1 cut(s)	106
PflMI	CCANNNNNTGG	1 cut(s)	574
PfoI	TCCNGGA	2 cut(s)	567, 588
PkrI	GCNGC	6 cut(s)	42, 209, 261, 667, 1045, 1102
PleI	GAGTC	5 cut(s)	23, 57, 115, 258, 731
PluTI	GGCGCC	1 cut(s)	302
PpsI	GAGTC	5 cut(s)	23, 57, 115, 258, 731
PsiI	TTATAA	1 cut(s)	1007
Psp124BI	GAGCTC	1 cut(s)	922
Psp6I	CCWGG	2 cut(s)	567, 588
PspEI	GGTNACC	1 cut(s)	100
PspGI	CCWGG	2 cut(s)	567, 588
PspN4I	GGNNCC	2 cut(s)	300, 324
PspPI	GGNCC	4 cut(s)	4, 819, 1133, 1208
PstNI	CAGNNNCTG	1 cut(s)	55
PsyI	GACNNNGTC	1 cut(s)	106
PteI	GCGCGC	1 cut(s)	1165
RsaI	GTAC	1 cut(s)	26
RsaNI	GTAC	1 cut(s)	25
SacI	GAGCTC	1 cut(s)	922
SalI	GTCGAC	1 cut(s)	109
SapI	GCTCTTC	1 cut(s)	197
SaqAI	TTAA	4 cut(s)	441, 728, 782, 1270
SatI	GCNGC	6 cut(s)	41, 208, 260, 666, 1044, 1101
Sau3AI	GATC	7 cut(s)	15, 84, 340, 380, 411, 960, 979
Sau96I	GGNCC	4 cut(s)	4, 819, 1133, 1208
SchI	GAGTC	5 cut(s)	23, 57, 116, 258, 732
ScrFI	CCNGG	2 cut(s)	569, 590
SduI	GDGCHC	4 cut(s)	493, 922, 1003, 1072
SfaNI	GCATC	6 cut(s)	281, 472, 785, 822, 1152, 1263
SfcI	CTRYAG	1 cut(s)	735
SfoI	GGCGCC	1 cut(s)	300
SinI	GGWCC	2 cut(s)	819, 1133
SmlI	CTYRAG	3 cut(s)	727, 1073, 1196
SmoI	CTYRAG	3 cut(s)	727, 1073, 1196
SsiI	CCGC	5 cut(s)	7, 198, 207, 837, 1193
SspDI	GGCGCC	1 cut(s)	298
SspMI	CTAG	2 cut(s)	635, 653
SstI	GAGCTC	1 cut(s)	922
StyD4I	CCNGG	2 cut(s)	567, 588
StyI	CCWWGG	2 cut(s)	609, 822
TaaI	ACNGT	3 cut(s)	388, 793, 865
TaiI	ACGT	3 cut(s)	314, 699, 1041
TaqI	TCGA	5 cut(s)	75, 110, 131, 414, 963
TaqII	GACCGA	2 cut(s)	119, 476
TauI	GCSGC	1 cut(s)	210
TfiI	GAWTC	2 cut(s)	72, 1085
Tru1I	TTAA	4 cut(s)	441, 728, 782, 1270
Tru9I	TTAA	4 cut(s)	441, 728, 782, 1270
TscAI	CASTG	3 cut(s)	501, 667, 796
TseFI	GTSAC	1 cut(s)	100
TseI	GCWGC	5 cut(s)	40, 259, 665, 1043, 1100
Tsp45I	GTSAC	1 cut(s)	100
TspDTI	ATGAA	5 cut(s)	319, 532, 611, 740, 777
TspGWI	ACGGA	1 cut(s)	128
TspRI	CASTG	3 cut(s)	501, 667, 796
Tth111I	GACNNNGTC	1 cut(s)	106
Van91I	CCANNNNNTGG	1 cut(s)	574
Vha464I	CTTAAG	1 cut(s)	727
VpaK11BI	GGWCC	2 cut(s)	819, 1133
XapI	RAATTY	3 cut(s)	347, 785, 1230
XceI	RCATGY	1 cut(s)	184
XcmI	CCANNNNNNNNNTGG	1 cut(s)	511
XmiI	GTMKAC	2 cut(s)	110, 155
XspI	CTAG	2 cut(s)	635, 653
Zsp2I	ATGCAT	1 cut(s)	1113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.