MD13G1152200.v1.1

GDSL esterase lipase At4g16230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0019746
Physical Location & Seq
Reverse (-)
11920367 .. 11931976
11610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1152200.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGAGGATTTTATTCCAAATGGTTACCTGCTCATCAGCCAAAAATGTTCCTGCCTTTTTCACTTTTGGCGACTCCTTAGTTGATGTGGGAAACAACAACTACCTTCAAACTGTAGCAAAGGGAAACTTTTATCCCTATGGAATTGATTTTGGGAATCCAACAGGACGATTTACAAACGGGAGAACAGTTTTCGACATTCTTCAACAGGAGTTGGGTTTTAAAAATTTTACTCCTCCTTACTTGGCACCAACTACGGTTGGAGAGACATGCTTCTCCAGGGTGTCAATTATGCTTCCAGTGCGTCCGGAATTTCCAACTCCACTGGGGGATCGAATCAATTTTGAGACGCAAGTCAGTTACCATGGGAGGACTGCTGCAGCTATCATGTCGAGGATTGGTATTCCTGCAGCTCAGAAATTGTTGAGAAGAGCAATTTATGGCATCGCAATTGGCTCTAACGACATAATGTTTAGGGAGACATCTCCCAAGCCTATGTCAGACAAAGCTTTTCTGGATTTATTGATTTCAAGACTCAAATTAGGACTCAGGACACTCTACAACTTGAATCCAAGAAAGTTTATCGTCGGTAATGCTGGACCAGGTGGGTGCATCCCATGGGAGAAAGATTTACATTTCCTTCCAGGGGGAACTTGTGCTCGTGTAGTAAATAAGATGGCCCGGATGTACAACAACAAGTTGAAGCGCTTGCTCGTTGAGCTTAACAAGGATCTCAAAGGGGCAAACTTTGTTTATGCAGATATTTACCAGATATTAACAGATCTCACCGAAAATTATGTATCATACGGTTTCTTAGGGTTTGAGAATGGAATTTCTGCATGCTGCTCAACGGCCGGGCCCCGAGGAGGACTAATCCTATGTAATCCATTAGCTAAAGTTTGTCCGGACAGATCAAAGTATGTATTCTGGGATCCAGATTACCAGCCTCTGTTAAGTGGAATGGATCCCAGAATACATACTTTGATCTGTCAGCAACTTGCCTTCAAAGGTTTCACTCCTCCTTACTTATCTCCAACAACTGCCGGGCCGAAAATTCTGCAGGGTATAAATTATGCTTCTGGTGGAGGTGGAATCCTTAATCACACAGGGAAGATTTTTGGAGGTCGATTAAACATGGATGAACAGCTAGATTTCTTTGCTAATACCAGGCAAGACATTATCTCCAGCTTTGGCCTTCCTGCAGCTCTGGAGCAATTAAGCAAGGCTCTCTTCTCCGTAACAATGGGGTCGAACGATTTCATTAACAACTACTTGACGCCGGTGATCTCAGCTCCTGAGCAAAAATTGATACCTCCGGAAACATTTGTGGGAATTTTGATTTCAATATACAGATTACAACTTACAAGGGATACAAATCTTGATGCAGGAGACTCCTGTGTCGAAAAACCAAATCAGTTTGCGCAATTGTTCAACCCCGAGTTGAAGAGCCTTCTCACGGAGCTTAACGCCAACCTTGAGGGGTCCAAATTTGTTTATGCAGATGTTTATCGCATTGTAGATGACATTACACAGAACTACATATCATATGGTGAGAATGTTGAGAATGAGTTCTACATCGAAGGAAGGAGGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

531

Amino Acids

58.87

Weight (kDa)

8.35

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 20 - 310 7.7e-16 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL PF00657 338 - 509 9.5e-09 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1394
Acc16I TGCGCA 1 cut(s) 1419
Acc36I ACCTGC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 244
AccIII TCCGGA 3 cut(s) 304, 901, 1312
AclWI GGATC 6 cut(s) 336, 735, 923, 936, 956, 969
AcoI YGGCCR 1 cut(s) 849
AcsI RAATTY 6 cut(s) 223, 308, 828, 1050, 1329, 1484
AcyI GRCGYC 1 cut(s) 1274
AfaI GTAC 1 cut(s) 686
AfeI AGCGCT 1 cut(s) 704
AfiI CCNNNNNNNGG 3 cut(s) 643, 863, 1453
AgsI TTSAA 9 cut(s) 107, 203, 528, 565, 700, 1003, 1341, 1429, 1441
AjnI CCWGG 4 cut(s) 275, 598, 640, 1163
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 4 cut(s) 257, 338, 470, 1380
AlwI GGATC 6 cut(s) 336, 735, 923, 936, 956, 969
AlwNI CAGNNNCTG 2 cut(s) 946, 1292
Ama87I CYCGRG 2 cut(s) 858, 1433
Aor13HI TCCGGA 3 cut(s) 304, 901, 1312
Aor51HI AGCGCT 1 cut(s) 704
AoxI GGCC 5 cut(s) 675, 849, 854, 1043, 1189
ApaI GGGCCC 1 cut(s) 858
ApeKI GCWGC 5 cut(s) 374, 377, 407, 840, 1199
ApoI RAATTY 6 cut(s) 223, 308, 828, 1050, 1329, 1484
Asp700I GAANNNNTTC 2 cut(s) 1253, 1565
AspLEI GCGC 2 cut(s) 705, 1420
AspS9I GGNCC 6 cut(s) 596, 676, 854, 855, 1043, 1479
AsuC2I CCSGG 3 cut(s) 679, 853, 1042
AsuHPI GGTGA 3 cut(s) 775, 1291, 1559
AvaI CYCGRG 2 cut(s) 858, 1433
AvaII GGWCC 2 cut(s) 596, 1479
BaeGI GKGCMC 1 cut(s) 858
BamHI GGATCC 2 cut(s) 928, 961
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 1 cut(s) 858
BauI CACGAG 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 5 cut(s) 361, 389, 419, 827, 1211
BccI CCATC 1 cut(s) 667
BceAI ACGGC 1 cut(s) 864
BcgI CGANNNNNNTGC 2 cut(s) 1036, 1070
BciT130I CCWGG 4 cut(s) 277, 600, 642, 1165
BciVI GTATCC 1 cut(s) 1360
BcnI CCSGG 3 cut(s) 679, 853, 1042
BcoDI GTCTC 4 cut(s) 257, 338, 470, 1380
BfaI CTAG 1 cut(s) 1145
BfmI CTRYAG 5 cut(s) 111, 375, 405, 1055, 1197
BfoI RGCGCY 1 cut(s) 706
BfuAI ACCTGC 1 cut(s) 35
BfuI GTATCC 1 cut(s) 1360
BglII AGATCT 1 cut(s) 778
BisI GCNGC 5 cut(s) 375, 378, 408, 841, 1200
BlsI GCNGC 5 cut(s) 376, 379, 409, 842, 1201
Bme1390I CCNGG 7 cut(s) 277, 600, 642, 679, 853, 1042, 1165
Bme18I GGWCC 2 cut(s) 596, 1479
BmeT110I CYCGRG 2 cut(s) 858, 1433
BmgT120I GGNCC 6 cut(s) 596, 676, 854, 855, 1043, 1479
BmiI GGNNCC 6 cut(s) 246, 856, 857, 930, 963, 1480
BmrFI CCNGG 7 cut(s) 277, 600, 642, 679, 853, 1042, 1165
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 3 cut(s) 450, 618, 1369
BmuI ACTGGG 1 cut(s) 332
BoxI GACNNNNGTC 1 cut(s) 350
BpmI CTGGAG 3 cut(s) 259, 1165, 1226
Bpu10I CCTNAGC 1 cut(s) 1293
BpuEI CTTGAG 1 cut(s) 1493
BpuMI CCSGG 3 cut(s) 679, 853, 1042
BsaBI GATNNNNATC 2 cut(s) 1371, 1503
BsaHI GRCGYC 1 cut(s) 1274
BsaJI CCNNGG 5 cut(s) 276, 361, 614, 641, 859
BsaWI WCCGGW 3 cut(s) 304, 901, 1312
BsaXI ACNNNNNCTCC 2 cut(s) 1163, 1193
Bsc4I CCNNNNNNNGG 3 cut(s) 643, 863, 1453
Bse118I RCCGGY 1 cut(s) 1276
Bse1I ACTGG 2 cut(s) 296, 327
Bse8I GATNNNNATC 2 cut(s) 1371, 1503
BseAI TCCGGA 3 cut(s) 304, 901, 1312
BseBI CCWGG 4 cut(s) 277, 600, 642, 1165
BseDI CCNNGG 5 cut(s) 276, 361, 614, 641, 859
BseGI GGATG 3 cut(s) 609, 687, 1141
BseJI GATNNNNATC 2 cut(s) 1371, 1503
BseLI CCNNNNNNNGG 3 cut(s) 643, 863, 1453
BseMII CTCAG 4 cut(s) 425, 559, 1284, 1299
BseNI ACTGG 2 cut(s) 296, 327
BseRI GAGGAG 3 cut(s) 222, 876, 1005
BseSI GKGCMC 1 cut(s) 858
BseX3I CGGCCG 1 cut(s) 849
BseXI GCAGC 5 cut(s) 361, 389, 419, 827, 1211
Bsh1285I CGRYCG 1 cut(s) 852
BshFI GGCC 5 cut(s) 677, 851, 856, 1045, 1191
BshNI GGYRCC 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 852
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 2 cut(s) 858, 1433
BsiSI CCGG 7 cut(s) 305, 679, 852, 902, 1041, 1277, 1313
BslI CCNNNNNNNGG 3 cut(s) 643, 863, 1453
BsmAI GTCTC 4 cut(s) 257, 338, 470, 1380
BsmBI CGTCTC 1 cut(s) 338
BsnI GGCC 5 cut(s) 677, 851, 856, 1045, 1191
BsoBI CYCGRG 2 cut(s) 858, 1433
Bsp120I GGGCCC 1 cut(s) 854
Bsp1286I GDGCHC 2 cut(s) 658, 858
Bsp13I TCCGGA 3 cut(s) 304, 901, 1312
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 8 cut(s) 328, 727, 778, 908, 928, 961, 981, 1281
Bsp19I CCATGG 2 cut(s) 361, 614
BspANI GGCC 5 cut(s) 677, 851, 856, 1045, 1191
BspCNI CTCAG 4 cut(s) 424, 558, 1285, 1298
BspEI TCCGGA 3 cut(s) 304, 901, 1312
BspLI GGNNCC 6 cut(s) 246, 856, 857, 930, 963, 1480
BspMAI CTGCAG 4 cut(s) 379, 409, 1059, 1201
BspMI ACCTGC 1 cut(s) 35
BspPI GGATC 6 cut(s) 336, 735, 923, 936, 956, 969
BspQI GCTCTTC 2 cut(s) 421, 1436
BspT107I GGYRCC 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 1276
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 2 cut(s) 296, 327
BssAI RCCGGY 1 cut(s) 1276
BssECI CCNNGG 5 cut(s) 276, 361, 614, 641, 859
BssMI GATC 8 cut(s) 328, 727, 778, 908, 928, 961, 981, 1281
BssNI GRCGYC 1 cut(s) 1274
BssSI CACGAG 1 cut(s) 657
BssT1I CCWWGG 2 cut(s) 361, 614
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 4 cut(s) 277, 600, 642, 1165
Bst4CI ACNGT 4 cut(s) 112, 187, 256, 806
Bst6I CTCTTC 3 cut(s) 421, 1232, 1436
BstACI GRCGYC 1 cut(s) 1274
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 2 cut(s) 707, 838
BstDEI CTNAG 6 cut(s) 76, 411, 545, 811, 1285, 1293
BstDSI CCRYGG 2 cut(s) 361, 614
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 3 cut(s) 609, 687, 1141
BstH2I RGCGCY 1 cut(s) 706
BstHHI GCGC 2 cut(s) 705, 1420
BstKTI GATC 8 cut(s) 331, 730, 781, 911, 931, 964, 984, 1284
BstMAI GTCTC 4 cut(s) 257, 338, 470, 1380
BstMBI GATC 8 cut(s) 328, 727, 778, 908, 928, 961, 981, 1281
BstMCI CGRYCG 1 cut(s) 852
BstMWI GCNNNNNNNGC 2 cut(s) 298, 715
BstNI CCWGG 4 cut(s) 277, 600, 642, 1165
BstNSI RCATGY 2 cut(s) 270, 840
BstPAI GACNNNNGTC 1 cut(s) 350
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 7 cut(s) 275, 598, 640, 677, 851, 1040, 1163
BstSFI CTRYAG 5 cut(s) 111, 375, 405, 1055, 1197
BstSLI GKGCMC 1 cut(s) 858
BstV1I GCAGC 5 cut(s) 361, 389, 419, 827, 1211
BstX2I RGATCY 4 cut(s) 727, 778, 928, 961
BstYI RGATCY 4 cut(s) 727, 778, 928, 961
BstZI CGGCCG 1 cut(s) 849
BsuI GTATCC 1 cut(s) 1360
BsuRI GGCC 5 cut(s) 677, 851, 856, 1045, 1191
BtgI CCRYGG 2 cut(s) 361, 614
BtgZI GCGATG 1 cut(s) 427
BtsCI GGATG 3 cut(s) 609, 687, 1141
BtsIMutI CAGTG 2 cut(s) 303, 320
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 707, 838
CaiI CAGNNNCTG 2 cut(s) 946, 1292
CfoI GCGC 2 cut(s) 705, 1420
Cfr10I RCCGGY 1 cut(s) 1276
Cfr13I GGNCC 6 cut(s) 596, 676, 854, 855, 1043, 1479
CseI GACGC 3 cut(s) 290, 355, 1282
CsiI ACCWGGT 1 cut(s) 598
Csp6I GTAC 1 cut(s) 685
CviAII CATG 6 cut(s) 267, 362, 385, 615, 837, 1132
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 6 cut(s) 76, 411, 545, 811, 1285, 1293
DpnI GATC 8 cut(s) 330, 729, 780, 910, 930, 963, 983, 1283
DpnII GATC 8 cut(s) 328, 727, 778, 908, 928, 961, 981, 1281
DraI TTTAAA 1 cut(s) 220
DrdI GACNNNNNNGTC 1 cut(s) 1394
DseDI GACNNNNNNGTC 1 cut(s) 1394
EaeI YGGCCR 1 cut(s) 849
EagI CGGCCG 1 cut(s) 849
Eam1104I CTCTTC 3 cut(s) 421, 1232, 1436
EarI CTCTTC 3 cut(s) 421, 1232, 1436
EclXI CGGCCG 1 cut(s) 849
Eco130I CCWWGG 2 cut(s) 361, 614
Eco24I GRGCYC 1 cut(s) 858
Eco47I GGWCC 2 cut(s) 596, 1479
Eco47III AGCGCT 1 cut(s) 704
Eco52I CGGCCG 1 cut(s) 849
Eco88I CYCGRG 2 cut(s) 858, 1433
Eco91I GGTNACC 1 cut(s) 22
EcoO109I RGGNCCY 1 cut(s) 855
EcoO65I GGTNACC 1 cut(s) 22
EcoRII CCWGG 4 cut(s) 275, 598, 640, 1163
EcoT14I CCWWGG 2 cut(s) 361, 614
EcoT38I GRGCYC 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 361, 614
Esp3I CGTCTC 1 cut(s) 338
FaeI CATG 6 cut(s) 270, 365, 388, 618, 840, 1135
FalI AAGNNNNNCTT 4 cut(s) 110, 142, 1211, 1243
FatI CATG 6 cut(s) 266, 361, 384, 614, 836, 1131
FauNDI CATATG 1 cut(s) 1543
Fnu4HI GCNGC 5 cut(s) 375, 378, 408, 841, 1200
FokI GGATG 3 cut(s) 596, 694, 1148
FriOI GRGCYC 1 cut(s) 858
Fsp4HI GCNGC 5 cut(s) 375, 378, 408, 841, 1200
FspBI CTAG 1 cut(s) 1145
FspI TGCGCA 1 cut(s) 1419
GlaI GCGC 2 cut(s) 704, 1419
GluI GCNGC 5 cut(s) 375, 378, 408, 841, 1200
GsuI CTGGAG 3 cut(s) 259, 1165, 1226
HaeII RGCGCY 1 cut(s) 706
HaeIII GGCC 5 cut(s) 677, 851, 856, 1045, 1191
HapII CCGG 7 cut(s) 305, 679, 852, 902, 1041, 1277, 1313
HgaI GACGC 3 cut(s) 290, 355, 1282
HhaI GCGC 2 cut(s) 705, 1420
Hin1I GRCGYC 1 cut(s) 1274
Hin1II CATG 6 cut(s) 270, 365, 388, 618, 840, 1135
Hin6I GCGC 2 cut(s) 703, 1418
HinP1I GCGC 2 cut(s) 703, 1418
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 8 cut(s) 71, 154, 333, 531, 543, 565, 1089, 1388
HpaII CCGG 7 cut(s) 305, 679, 852, 902, 1041, 1277, 1313
HphI GGTGA 3 cut(s) 775, 1291, 1559
Hpy188I TCNGA 2 cut(s) 414, 499
Hpy99I CGWCG 1 cut(s) 587
HpyAV CCTTC 7 cut(s) 113, 647, 1009, 1202, 1457, 1571, 1575
HpyCH4III ACNGT 4 cut(s) 112, 187, 256, 806
HpyCH4V TGCA 9 cut(s) 377, 407, 609, 755, 836, 1057, 1199, 1382, 1496
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 715
HpyF3I CTNAG 6 cut(s) 76, 411, 545, 811, 1285, 1293
Hsp92I GRCGYC 1 cut(s) 1274
Hsp92II CATG 6 cut(s) 270, 365, 388, 618, 840, 1135
HspAI GCGC 2 cut(s) 703, 1418
Kpn2I TCCGGA 3 cut(s) 304, 901, 1312
Kzo9I GATC 8 cut(s) 328, 727, 778, 908, 928, 961, 981, 1281
LguI GCTCTTC 2 cut(s) 421, 1436
LmnI GCTCC 3 cut(s) 1207, 1294, 1456
Lsp1109I GCAGC 5 cut(s) 361, 389, 419, 827, 1211
LweI GCATC 3 cut(s) 450, 618, 1369
MabI ACCWGGT 1 cut(s) 598
MaeI CTAG 1 cut(s) 1145
MaeIII GTNAC 3 cut(s) 22, 356, 1234
MalI GATC 8 cut(s) 330, 729, 780, 910, 930, 963, 983, 1283
MboI GATC 8 cut(s) 328, 727, 778, 908, 928, 961, 981, 1281
MboII GAAGA 5 cut(s) 191, 438, 1120, 1219, 1453
MfeI CAATTG 2 cut(s) 447, 1421
MflI RGATCY 4 cut(s) 727, 778, 928, 961
MhlI GDGCHC 2 cut(s) 658, 858
MlyI GAGTC 4 cut(s) 65, 525, 537, 1382
MmeI TCCRAC 4 cut(s) 182, 238, 338, 1055
MroI TCCGGA 3 cut(s) 304, 901, 1312
MroXI GAANNNNTTC 2 cut(s) 1253, 1565
MseI TTAA 9 cut(s) 219, 720, 773, 950, 1095, 1127, 1214, 1260, 1461
MspI CCGG 7 cut(s) 305, 679, 852, 902, 1041, 1277, 1313
MspR9I CCNGG 7 cut(s) 277, 600, 642, 679, 853, 1042, 1165
MunI CAATTG 2 cut(s) 447, 1421
MvaI CCWGG 4 cut(s) 277, 600, 642, 1165
MwoI GCNNNNNNNGC 2 cut(s) 298, 715
NciI CCSGG 3 cut(s) 679, 853, 1042
NcoI CCATGG 2 cut(s) 361, 614
NdeI CATATG 1 cut(s) 1543
NdeII GATC 8 cut(s) 328, 727, 778, 908, 928, 961, 981, 1281
NlaIII CATG 6 cut(s) 270, 365, 388, 618, 840, 1135
NlaIV GGNNCC 6 cut(s) 246, 856, 857, 930, 963, 1480
NsbI TGCGCA 1 cut(s) 1419
NspI RCATGY 2 cut(s) 270, 840
PaeI GCATGC 1 cut(s) 840
PciSI GCTCTTC 2 cut(s) 421, 1436
PdmI GAANNNNTTC 2 cut(s) 1253, 1565
PfeI GAWTC 4 cut(s) 154, 333, 565, 1089
PkrI GCNGC 5 cut(s) 376, 379, 409, 842, 1201
PleI GAGTC 4 cut(s) 65, 525, 537, 1382
PpsI GAGTC 4 cut(s) 65, 525, 537, 1382
PshAI GACNNNNGTC 1 cut(s) 350
Psp6I CCWGG 4 cut(s) 275, 598, 640, 1163
PspEI GGTNACC 1 cut(s) 22
PspGI CCWGG 4 cut(s) 275, 598, 640, 1163
PspN4I GGNNCC 6 cut(s) 246, 856, 857, 930, 963, 1480
PspOMI GGGCCC 1 cut(s) 854
PspPI GGNCC 6 cut(s) 596, 676, 854, 855, 1043, 1479
PstI CTGCAG 4 cut(s) 379, 409, 1059, 1201
PstNI CAGNNNCTG 2 cut(s) 946, 1292
PsuI RGATCY 4 cut(s) 727, 778, 928, 961
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
SapI GCTCTTC 2 cut(s) 421, 1436
SaqAI TTAA 9 cut(s) 219, 720, 773, 950, 1095, 1127, 1214, 1260, 1461
SatI GCNGC 5 cut(s) 375, 378, 408, 841, 1200
Sau3AI GATC 8 cut(s) 328, 727, 778, 908, 928, 961, 981, 1281
Sau96I GGNCC 6 cut(s) 596, 676, 854, 855, 1043, 1479
SchI GAGTC 4 cut(s) 65, 525, 537, 1382
ScrFI CCNGG 7 cut(s) 277, 600, 642, 679, 853, 1042, 1165
SduI GDGCHC 2 cut(s) 658, 858
SexAI ACCWGGT 1 cut(s) 598
SfaNI GCATC 3 cut(s) 450, 618, 1369
SfcI CTRYAG 5 cut(s) 111, 375, 405, 1055, 1197
SgrAI CRCCGGYG 1 cut(s) 1276
SinI GGWCC 2 cut(s) 596, 1479
SmlI CTYRAG 1 cut(s) 1472
SmoI CTYRAG 1 cut(s) 1472
SphI GCATGC 1 cut(s) 840
SspMI CTAG 1 cut(s) 1145
StyD4I CCNGG 7 cut(s) 275, 598, 640, 677, 851, 1040, 1163
StyI CCWWGG 2 cut(s) 361, 614
TaaI ACNGT 4 cut(s) 112, 187, 256, 806
TaqI TCGA 7 cut(s) 192, 331, 389, 1123, 1247, 1398, 1575
TatI WGTACW 1 cut(s) 684
TfiI GAWTC 4 cut(s) 154, 333, 565, 1089
Tru1I TTAA 9 cut(s) 219, 720, 773, 950, 1095, 1127, 1214, 1260, 1461
Tru9I TTAA 9 cut(s) 219, 720, 773, 950, 1095, 1127, 1214, 1260, 1461
TscAI CASTG 2 cut(s) 303, 327
TseI GCWGC 5 cut(s) 374, 377, 407, 840, 1199
TspDTI ATGAA 2 cut(s) 1152, 1246
TspGWI ACGGA 2 cut(s) 1222, 1469
TspRI CASTG 2 cut(s) 303, 327
VpaK11BI GGWCC 2 cut(s) 596, 1479
XapI RAATTY 6 cut(s) 223, 308, 828, 1050, 1329, 1484
XceI RCATGY 2 cut(s) 270, 840
XmnI GAANNNNTTC 2 cut(s) 1253, 1565
XspI CTAG 1 cut(s) 1145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.