MD13G1152500.v1.1

GDSL esterase lipase At4g16230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0019746
Physical Location & Seq
Reverse (-)
11945245 .. 11947678
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1152500.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGATGTGGATTATATTCCAAACTGTTACAGTCTACTTCTTGTTAGTTACTTGCTCAGCCAAAAATAATTTTCCTGCTTTTTTAACTTTTGCCAATTCCTTGGCTGATGTGGGAAACAACAACTACCTCCCAACCATAGTAAAGGGAAACTTTTACCCCAATGGAATTGATTTTGGGAAGCCAACAGGACGATTTACGAATGGGAGAACAGTTTTCGACATTCTCCAGCAGGAAATGGGTTTTAAAAATTTTACTCCTCCTTACTTGGCACCAACTACAGTTGGAGACATGCTTCTACAAGGTGTTAATTATGCTTCCTCTGCCAGTGGAATTTCAAATTCCACTGAAACATTTTATGGCACTTTACTCAATTTCGATACACAAATCAGTTACCATGGGAGGACTGCGATAGCAATCATGTCAAGGATTGGTATTCCTGCAGCTCAGAAATTGTTGAGTAGAGCAATTTATGCGATTGCAATCGGCTCAAACGATATTATGTATTCCGAGACTTCTTCCATGAGCGAAGAAGATTATTTCGATTTCTTGATTTCAAGACTCAAATTAGGACTTAAGACACTCCACAGCTTGGATGCTAGAAAGATTGTTGTGGCTAATGCTGGACTTATTGTGTGTATCCCATATGAGAAAGATATACATCCAGTTCCAAAGGGAAGTTGTGTTCGTATACTAAATCGTACCGCCCAGAGGTATAACGCCAAGTTGAAGGGCCTGCTCGTGGAGCTCAACAAGAATCTCAAAGGGGCAAAGTTTATTTATGCAGATATTTACCAAATGTTGGAAGACCTCTCTAACAATTACGTGTCATATGGGTTTGAGAATGGCGTTTCTGCTTGCTGCTCCGTTGCCGGGCCCCAAGGGGGACTAATCCCGTGTACCTTTTTGTCTAAAATTTGTCCCGACAGATCAAAGTATTTATTCTGGGATCCATTCCACTTAACGGAGGCTGGTAATCTCATTGCAGCAAGGCATATGATGGATGGTAACTCAACTTATATGTCACCAATGAATATAAGGCAACTTCTGCAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

352

Amino Acids

38.92

Weight (kDa)

8.7

Isoelectric Point (pI)

33.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 331 9.3e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 268
AccB7I CCANNNNNTGG 2 cut(s) 589, 799
AccI GTMKAC 2 cut(s) 33, 688
AciI CCGC 1 cut(s) 702
AclWI GGATC 2 cut(s) 941, 954
AcsI RAATTY 4 cut(s) 247, 330, 337, 912
AfaI GTAC 2 cut(s) 700, 898
AfiI CCNNNNNNNGG 7 cut(s) 589, 708, 739, 799, 870, 881, 961
AflII CTTAAG 1 cut(s) 572
AflIII ACRYGT 1 cut(s) 822
AgsI TTSAA 3 cut(s) 336, 555, 727
AloI GAACNNNNNNTCC 2 cut(s) 666, 698
AluBI AGCT 3 cut(s) 443, 588, 745
AluI AGCT 3 cut(s) 443, 588, 745
Alw21I GWGCWC 1 cut(s) 747
Alw26I GTCTC 2 cut(s) 279, 503
AlwI GGATC 2 cut(s) 941, 954
AoxI GGCC 2 cut(s) 730, 872
ApaI GGGCCC 1 cut(s) 876
ApeKI GCWGC 3 cut(s) 440, 858, 983
ApoI RAATTY 4 cut(s) 247, 330, 337, 912
AspS9I GGNCC 3 cut(s) 730, 872, 873
AsuC2I CCSGG 1 cut(s) 871
AsuHPI GGTGA 1 cut(s) 1014
BaeGI GKGCMC 1 cut(s) 876
BamHI GGATCC 1 cut(s) 946
BanI GGYRCC 1 cut(s) 268
BanII GRGCYC 2 cut(s) 747, 876
BauI CACGAG 1 cut(s) 737
BbsI GAAGAC 1 cut(s) 810
Bbv12I GWGCWC 1 cut(s) 747
BbvI GCAGC 3 cut(s) 452, 845, 995
BccI CCATC 2 cut(s) 991, 995
BciVI GTATCC 1 cut(s) 647
BcnI CCSGG 1 cut(s) 871
BcoDI GTCTC 2 cut(s) 279, 503
BfaI CTAG 1 cut(s) 597
BfmI CTRYAG 2 cut(s) 276, 438
BfrI CTTAAG 1 cut(s) 572
BfuI GTATCC 1 cut(s) 647
BisI GCNGC 3 cut(s) 441, 859, 984
BlpI GCTNAGC 1 cut(s) 55
BlsI GCNGC 3 cut(s) 442, 860, 985
Bme1390I CCNGG 1 cut(s) 871
BmgT120I GGNCC 3 cut(s) 730, 872, 873
BmiI GGNNCC 4 cut(s) 270, 874, 875, 948
BmrFI CCNGG 1 cut(s) 871
BmsI GCATC 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 810
BpmI CTGGAG 1 cut(s) 209
Bpu1102I GCTNAGC 1 cut(s) 55
BpuMI CCSGG 1 cut(s) 871
BsaAI YACGTR 1 cut(s) 823
BsaBI GATNNNNATC 3 cut(s) 413, 479, 657
BsaJI CCNNGG 3 cut(s) 99, 394, 877
Bsc4I CCNNNNNNNGG 7 cut(s) 589, 708, 739, 799, 870, 881, 961
Bse1I ACTGG 2 cut(s) 324, 662
Bse3DI GCAATG 1 cut(s) 978
Bse8I GATNNNNATC 3 cut(s) 413, 479, 657
BseDI CCNNGG 3 cut(s) 99, 394, 877
BseGI GGATG 3 cut(s) 598, 658, 1006
BseJI GATNNNNATC 3 cut(s) 413, 479, 657
BseLI CCNNNNNNNGG 7 cut(s) 589, 708, 739, 799, 870, 881, 961
BseMI GCAATG 1 cut(s) 978
BseMII CTCAG 2 cut(s) 69, 458
BseNI ACTGG 2 cut(s) 324, 662
BseRI GAGGAG 1 cut(s) 246
BseSI GKGCMC 1 cut(s) 876
BseXI GCAGC 3 cut(s) 452, 845, 995
BshFI GGCC 2 cut(s) 732, 874
BshNI GGYRCC 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 747
BsiSI CCGG 1 cut(s) 870
BslFI GGGAC 2 cut(s) 897, 903
BslI CCNNNNNNNGG 7 cut(s) 589, 708, 739, 799, 870, 881, 961
BsmAI GTCTC 2 cut(s) 279, 503
BsmFI GGGAC 2 cut(s) 897, 903
BsnI GGCC 2 cut(s) 732, 874
Bsp120I GGGCCC 1 cut(s) 872
Bsp1286I GDGCHC 2 cut(s) 747, 876
Bsp143I GATC 2 cut(s) 926, 946
Bsp1720I GCTNAGC 1 cut(s) 55
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 1 cut(s) 702
BspANI GGCC 2 cut(s) 732, 874
BspCNI CTCAG 2 cut(s) 68, 457
BspLI GGNNCC 4 cut(s) 270, 874, 875, 948
BspMAI CTGCAG 1 cut(s) 442
BspPI GGATC 2 cut(s) 941, 954
BspT107I GGYRCC 1 cut(s) 268
BspTI CTTAAG 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 978
BsrI ACTGG 2 cut(s) 324, 662
BssECI CCNNGG 3 cut(s) 99, 394, 877
BssMI GATC 2 cut(s) 926, 946
BssNAI GTATAC 1 cut(s) 689
BssSI CACGAG 1 cut(s) 737
BssT1I CCWWGG 3 cut(s) 99, 394, 877
Bst1107I GTATAC 1 cut(s) 689
Bst2BI CACGAG 1 cut(s) 737
Bst4CI ACNGT 4 cut(s) 25, 31, 211, 280
BstAFI CTTAAG 1 cut(s) 572
BstAPI GCANNNNNTGC 2 cut(s) 470, 1045
BstBAI YACGTR 1 cut(s) 823
BstC8I GCNNGC 2 cut(s) 734, 856
BstDEI CTNAG 2 cut(s) 55, 444
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 3 cut(s) 598, 658, 1006
BstKTI GATC 2 cut(s) 929, 949
BstMAI GTCTC 2 cut(s) 279, 503
BstMBI GATC 2 cut(s) 926, 946
BstMWI GCNNNNNNNGC 4 cut(s) 320, 470, 742, 1045
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 869
BstSFI CTRYAG 2 cut(s) 276, 438
BstSLI GKGCMC 1 cut(s) 876
BstV1I GCAGC 3 cut(s) 452, 845, 995
BstV2I GAAGAC 1 cut(s) 810
BstX2I RGATCY 1 cut(s) 946
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 1 cut(s) 946
BstZ17I GTATAC 1 cut(s) 689
BsuI GTATCC 1 cut(s) 647
BsuRI GGCC 2 cut(s) 732, 874
BtgI CCRYGG 1 cut(s) 394
BtsCI GGATG 3 cut(s) 598, 658, 1006
BtsIMutI CAGTG 2 cut(s) 331, 342
Cac8I GCNNGC 2 cut(s) 734, 856
Cfr13I GGNCC 3 cut(s) 730, 872, 873
Csp6I GTAC 2 cut(s) 699, 897
CviAII CATG 4 cut(s) 289, 395, 418, 520
CviQI GTAC 2 cut(s) 699, 897
DdeI CTNAG 2 cut(s) 55, 444
DpnI GATC 2 cut(s) 928, 948
DpnII GATC 2 cut(s) 926, 946
DraI TTTAAA 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 745
Eco130I CCWWGG 3 cut(s) 99, 394, 877
Eco24I GRGCYC 2 cut(s) 747, 876
Eco53kI GAGCTC 1 cut(s) 745
EcoICRI GAGCTC 1 cut(s) 745
EcoO109I RGGNCCY 2 cut(s) 730, 873
EcoT14I CCWWGG 3 cut(s) 99, 394, 877
EcoT38I GRGCYC 2 cut(s) 747, 876
ErhI CCWWGG 3 cut(s) 99, 394, 877
FaeI CATG 4 cut(s) 292, 398, 421, 523
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 2 cut(s) 897, 903
FatI CATG 4 cut(s) 288, 394, 417, 519
FauNDI CATATG 3 cut(s) 643, 829, 993
FblI GTMKAC 2 cut(s) 33, 688
Fnu4HI GCNGC 3 cut(s) 441, 859, 984
FokI GGATG 3 cut(s) 605, 645, 1013
FriOI GRGCYC 2 cut(s) 747, 876
Fsp4HI GCNGC 3 cut(s) 441, 859, 984
FspBI CTAG 1 cut(s) 597
GluI GCNGC 3 cut(s) 441, 859, 984
GsuI CTGGAG 1 cut(s) 209
HaeIII GGCC 2 cut(s) 732, 874
HapII CCGG 1 cut(s) 870
Hin1II CATG 4 cut(s) 292, 398, 421, 523
HinfI GANTC 2 cut(s) 558, 754
HpaII CCGG 1 cut(s) 870
HphI GGTGA 1 cut(s) 1014
Hpy166II GTNNAC 3 cut(s) 34, 689, 897
Hpy188I TCNGA 2 cut(s) 447, 508
Hpy188III TCNNGA 3 cut(s) 547, 555, 920
Hpy8I GTNNAC 3 cut(s) 34, 689, 897
HpyAV CCTTC 1 cut(s) 721
HpyCH4III ACNGT 4 cut(s) 25, 31, 211, 280
HpyCH4IV ACGT 1 cut(s) 822
HpyCH4V TGCA 5 cut(s) 440, 479, 782, 983, 1048
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 470, 742, 1045
HpyF3I CTNAG 2 cut(s) 55, 444
HpySE526I ACGT 1 cut(s) 822
Hsp92II CATG 4 cut(s) 292, 398, 421, 523
Kzo9I GATC 2 cut(s) 926, 946
LmnI GCTCC 2 cut(s) 742, 866
Lsp1109I GCAGC 3 cut(s) 452, 845, 995
LweI GCATC 1 cut(s) 583
MaeI CTAG 1 cut(s) 597
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 5 cut(s) 25, 46, 389, 1004, 1020
MalI GATC 2 cut(s) 928, 948
MboI GATC 2 cut(s) 926, 946
MboII GAAGA 4 cut(s) 507, 539, 542, 815
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 2 cut(s) 747, 876
MlyI GAGTC 1 cut(s) 552
MmeI TCCRAC 2 cut(s) 262, 780
MnlI CCTC 7 cut(s) 137, 267, 328, 393, 702, 818, 958
MseI TTAA 6 cut(s) 83, 243, 306, 573, 959, 1054
MspCI CTTAAG 1 cut(s) 572
MspI CCGG 1 cut(s) 870
MspR9I CCNGG 1 cut(s) 871
MwoI GCNNNNNNNGC 4 cut(s) 320, 470, 742, 1045
NciI CCSGG 1 cut(s) 871
NcoI CCATGG 1 cut(s) 394
NdeI CATATG 3 cut(s) 643, 829, 993
NdeII GATC 2 cut(s) 926, 946
NlaIII CATG 4 cut(s) 292, 398, 421, 523
NlaIV GGNNCC 4 cut(s) 270, 874, 875, 948
NmuCI GTSAC 1 cut(s) 1020
NspI RCATGY 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PfeI GAWTC 1 cut(s) 754
PflMI CCANNNNNTGG 2 cut(s) 589, 799
PkrI GCNGC 3 cut(s) 442, 860, 985
PleI GAGTC 1 cut(s) 552
PpsI GAGTC 1 cut(s) 552
Ppu21I YACGTR 1 cut(s) 823
Psp124BI GAGCTC 1 cut(s) 747
PspN4I GGNNCC 4 cut(s) 270, 874, 875, 948
PspOMI GGGCCC 1 cut(s) 872
PspPI GGNCC 3 cut(s) 730, 872, 873
PstI CTGCAG 1 cut(s) 442
PsuI RGATCY 1 cut(s) 946
RsaI GTAC 2 cut(s) 700, 898
RsaNI GTAC 2 cut(s) 699, 897
SacI GAGCTC 1 cut(s) 747
SaqAI TTAA 6 cut(s) 83, 243, 306, 573, 959, 1054
SatI GCNGC 3 cut(s) 441, 859, 984
Sau3AI GATC 2 cut(s) 926, 946
Sau96I GGNCC 3 cut(s) 730, 872, 873
SchI GAGTC 1 cut(s) 552
ScrFI CCNGG 1 cut(s) 871
SduI GDGCHC 2 cut(s) 747, 876
SetI ASST 9 cut(s) 129, 304, 445, 590, 713, 747, 810, 825, 902
SfaNI GCATC 1 cut(s) 583
SfcI CTRYAG 2 cut(s) 276, 438
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
SsiI CCGC 1 cut(s) 702
SspMI CTAG 1 cut(s) 597
SstI GAGCTC 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 869
StyI CCWWGG 3 cut(s) 99, 394, 877
TaaI ACNGT 4 cut(s) 25, 31, 211, 280
TaiI ACGT 1 cut(s) 825
TaqI TCGA 3 cut(s) 216, 375, 540
TfiI GAWTC 1 cut(s) 754
Tru1I TTAA 6 cut(s) 83, 243, 306, 573, 959, 1054
Tru9I TTAA 6 cut(s) 83, 243, 306, 573, 959, 1054
TscAI CASTG 2 cut(s) 331, 349
TseFI GTSAC 1 cut(s) 1020
TseI GCWGC 3 cut(s) 440, 858, 983
Tsp45I GTSAC 1 cut(s) 1020
TspDTI ATGAA 1 cut(s) 1043
TspGWI ACGGA 2 cut(s) 853, 977
TspRI CASTG 2 cut(s) 331, 349
Van91I CCANNNNNTGG 2 cut(s) 589, 799
Vha464I CTTAAG 1 cut(s) 572
XapI RAATTY 4 cut(s) 247, 330, 337, 912
XceI RCATGY 1 cut(s) 292
XmiI GTMKAC 2 cut(s) 33, 688
XspI CTAG 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.