Prupe.1G116200_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0019746
Physical Location & Seq
Reverse (-)
9160210 .. 9161827
1618 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G116200.1

Sequence Viewer

Length: 1149 bp
ATGGGGATAATTATATTCCAAATTTTTGTAGTTTACTTGTTTTTGTTGGTTAGCTGCTCAGCCCAAAAAAATGTTCCTGCTGTTTTCACTTTTGGCGATTCCTTGGTTGATGTGGGAAACAACAACAACCTTCAAACTGTAGCTAAGGCAAATTTTCTCCCTCATGGACTTGATTTTGGGAATAACCCAACAGGACGATTTTCCAATGGGAGAATAGTTGTTGACATTATTCAAACGTATTCGAGTATGGACGCAAAGTTTTTACTATTTTCTTATTTTCTTATGACAGAACAGGAAATGGGTTTGAAAAATTTTACTCCACCTTACTTGGCTGCAACTGCTGCTGGAGACAATCTTCTACAAGGTGTCAATTATGTTTCCTCTGGCTCTGGAATTTCCAATTTCACTGGATTTCTCTTTGGTGATCGAATCAATTTCGACACGCAAATCAGTAACCATGGGAAAACTGCGCAAGCTATCATGTCAAGGATTGGCATTCCAGCAGCTCAGAAATTGTTGAGAAAAGCAATTTATGTGGTTGTAATTGGCTCTAACGACATAATGTTCAAGGAGTTTGAACCCATGGCTATGTCAGAAGAAGCTTACTTACATATATTGGCTGCAATAAAAAAGTTAGAAGCACACACACTAAGAAAAATTCCTTTGCAGACACTCTACAACTTGGATGCTAGAAAGATTATGGTGAGCAACGTTGCACTTGTTGGGTGTACCCCATTTGAGAAAGACATACATCCAGTTGAGAAGGGAAGTTGTGCTCGTATGCTAAATAAGATGGCCCAGATATACAACAAGAAGTTGAAGGGCATGCTTGCAGAGCTTAACAAAGAGCTTCAAGGGGCAAAGTTTGTTTATGCAGATATTTATCAAATATTGCAAGATCTCACCCAAAATTATGCGTCATATGAATTTGAGGTTGCAACTTCTGCATGCTGCGTGTTTGCTGGGAGCCGAGGGGGACTGGTACCCTGCAATCCTTTATCTAAAGTTTGTCCCAACAGATCAAAGTATGTGTTCTGGGATCCATTTCACTTGACGGATGCTGCTTGTGTTGTTGCAACGAGGCATATTATGGATAGCTACGTAACTTATATGTCACCTATGAATTTGAGACAGCTTGTGCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

383

Amino Acids

42.4

Weight (kDa)

8.64

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 471
Acc65I GGTACC 1 cut(s) 982
AccB1I GGYRCC 1 cut(s) 982
AclI AACGTT 1 cut(s) 711
AclWI GGATC 2 cut(s) 1034, 1047
AcsI RAATTY 7 cut(s) 21, 151, 310, 393, 657, 926, 1123
AfaI GTAC 2 cut(s) 730, 984
AgsI TTSAA 7 cut(s) 134, 233, 307, 568, 578, 820, 854
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AluBI AGCT 9 cut(s) 54, 143, 476, 506, 602, 838, 850, 1098, 1135
AluI AGCT 9 cut(s) 54, 143, 476, 506, 602, 838, 850, 1098, 1135
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 2 cut(s) 342, 1123
AlwI GGATC 2 cut(s) 1034, 1047
AoxI GGCC 1 cut(s) 795
ApeKI GCWGC 7 cut(s) 54, 332, 341, 503, 620, 951, 1061
ApoI RAATTY 7 cut(s) 21, 151, 310, 393, 657, 926, 1123
Asp718I GGTACC 1 cut(s) 982
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 1 cut(s) 796
AsuHPI GGTGA 4 cut(s) 434, 715, 895, 1107
BamHI GGATCC 1 cut(s) 1039
BanI GGYRCC 1 cut(s) 982
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
Bbv12I GWGCWC 1 cut(s) 778
BbvI GCAGC 7 cut(s) 41, 319, 328, 515, 607, 938, 1048
BccI CCATC 1 cut(s) 787
BcoDI GTCTC 2 cut(s) 342, 1123
BfaI CTAG 1 cut(s) 690
BfmI CTRYAG 1 cut(s) 138
BglII AGATCT 1 cut(s) 898
BisI GCNGC 7 cut(s) 55, 333, 342, 504, 621, 952, 1062
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 7 cut(s) 56, 334, 343, 505, 622, 953, 1063
BmgT120I GGNCC 1 cut(s) 796
BmiI GGNNCC 3 cut(s) 968, 984, 1041
BmsI GCATC 2 cut(s) 676, 1048
BpmI CTGGAG 1 cut(s) 366
Bpu10I CCTNAGC 1 cut(s) 144
Bpu1102I GCTNAGC 1 cut(s) 58
BsaAI YACGTR 1 cut(s) 1102
BsaBI GATNNNNATC 1 cut(s) 882
BsaJI CCNNGG 4 cut(s) 102, 457, 582, 970
Bse1I ACTGG 3 cut(s) 412, 755, 984
Bse8I GATNNNNATC 1 cut(s) 882
BseDI CCNNGG 4 cut(s) 102, 457, 582, 970
BseGI GGATG 3 cut(s) 691, 751, 1063
BseJI GATNNNNATC 1 cut(s) 882
BseMII CTCAG 2 cut(s) 72, 521
BseNI ACTGG 3 cut(s) 412, 755, 984
BseXI GCAGC 7 cut(s) 41, 319, 328, 515, 607, 938, 1048
BseYI CCCAGC 1 cut(s) 962
BshFI GGCC 1 cut(s) 797
BshNI GGYRCC 1 cut(s) 982
BsiHKAI GWGCWC 1 cut(s) 778
BslFI GGGAC 2 cut(s) 990, 996
BsmAI GTCTC 2 cut(s) 342, 1123
BsmFI GGGAC 2 cut(s) 990, 996
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 778
Bsp143I GATC 4 cut(s) 424, 898, 1019, 1039
Bsp1720I GCTNAGC 1 cut(s) 58
Bsp19I CCATGG 2 cut(s) 457, 582
BspANI GGCC 1 cut(s) 797
BspCNI CTCAG 2 cut(s) 71, 520
BspLI GGNNCC 3 cut(s) 968, 984, 1041
BspPI GGATC 2 cut(s) 1034, 1047
BspT107I GGYRCC 1 cut(s) 982
BsrI ACTGG 3 cut(s) 412, 755, 984
BssECI CCNNGG 4 cut(s) 102, 457, 582, 970
BssMI GATC 4 cut(s) 424, 898, 1019, 1039
BssT1I CCWWGG 3 cut(s) 102, 457, 582
Bst4CI ACNGT 1 cut(s) 139
BstAPI GCANNNNNTGC 2 cut(s) 341, 944
BstBAI YACGTR 1 cut(s) 1102
BstC8I GCNNGC 4 cut(s) 474, 827, 831, 949
BstDEI CTNAG 4 cut(s) 58, 144, 507, 650
BstDSI CCRYGG 2 cut(s) 457, 582
BstF5I GGATG 3 cut(s) 691, 751, 1063
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 4 cut(s) 427, 901, 1022, 1042
BstMAI GTCTC 2 cut(s) 342, 1123
BstMBI GATC 4 cut(s) 424, 898, 1019, 1039
BstMWI GCNNNNNNNGC 4 cut(s) 338, 341, 835, 944
BstNSI RCATGY 2 cut(s) 829, 951
BstSFI CTRYAG 1 cut(s) 138
BstSNI TACGTA 1 cut(s) 1102
BstV1I GCAGC 7 cut(s) 41, 319, 328, 515, 607, 938, 1048
BstX2I RGATCY 2 cut(s) 898, 1039
BstYI RGATCY 2 cut(s) 898, 1039
BsuRI GGCC 1 cut(s) 797
BtgI CCRYGG 2 cut(s) 457, 582
BtsCI GGATG 3 cut(s) 691, 751, 1063
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 4 cut(s) 474, 827, 831, 949
CfoI GCGC 1 cut(s) 472
Cfr13I GGNCC 1 cut(s) 796
CseI GACGC 2 cut(s) 260, 906
Csp6I GTAC 2 cut(s) 729, 983
CspCI CAANNNNNGTGG 4 cut(s) 309, 344, 516, 551
CviAII CATG 6 cut(s) 164, 458, 481, 583, 826, 948
CviQI GTAC 2 cut(s) 729, 983
DdeI CTNAG 4 cut(s) 58, 144, 507, 650
DpnI GATC 4 cut(s) 426, 900, 1021, 1041
DpnII GATC 4 cut(s) 424, 898, 1019, 1039
Eco105I TACGTA 1 cut(s) 1102
Eco130I CCWWGG 3 cut(s) 102, 457, 582
EcoT14I CCWWGG 3 cut(s) 102, 457, 582
ErhI CCWWGG 3 cut(s) 102, 457, 582
FaeI CATG 6 cut(s) 167, 461, 484, 586, 829, 951
FaqI GGGAC 2 cut(s) 990, 996
FatI CATG 6 cut(s) 163, 457, 480, 582, 825, 947
FauNDI CATATG 1 cut(s) 922
Fnu4HI GCNGC 7 cut(s) 55, 333, 342, 504, 621, 952, 1062
FokI GGATG 3 cut(s) 698, 738, 1070
Fsp4HI GCNGC 7 cut(s) 55, 333, 342, 504, 621, 952, 1062
FspBI CTAG 1 cut(s) 690
FspI TGCGCA 1 cut(s) 471
GlaI GCGC 1 cut(s) 471
GluI GCNGC 7 cut(s) 55, 333, 342, 504, 621, 952, 1062
GsaI CCCAGC 1 cut(s) 966
GsuI CTGGAG 1 cut(s) 366
HaeIII GGCC 1 cut(s) 797
HgaI GACGC 2 cut(s) 260, 906
HhaI GCGC 1 cut(s) 472
Hin1II CATG 6 cut(s) 167, 461, 484, 586, 829, 951
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HindIII AAGCTT 1 cut(s) 600
HinfI GANTC 2 cut(s) 98, 429
HphI GGTGA 4 cut(s) 434, 715, 895, 1107
Hpy166II GTNNAC 3 cut(s) 34, 223, 729
Hpy188I TCNGA 2 cut(s) 510, 595
Hpy188III TCNNGA 2 cut(s) 390, 1146
Hpy8I GTNNAC 3 cut(s) 34, 223, 729
HpyAV CCTTC 3 cut(s) 140, 757, 814
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 3 cut(s) 236, 711, 1101
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 341, 835, 944
HpyF3I CTNAG 4 cut(s) 58, 144, 507, 650
HpySE526I ACGT 3 cut(s) 236, 711, 1101
Hsp92II CATG 6 cut(s) 167, 461, 484, 586, 829, 951
HspAI GCGC 1 cut(s) 470
KpnI GGTACC 1 cut(s) 986
Kzo9I GATC 4 cut(s) 424, 898, 1019, 1039
LmnI GCTCC 1 cut(s) 966
Lsp1109I GCAGC 7 cut(s) 41, 319, 328, 515, 607, 938, 1048
LweI GCATC 2 cut(s) 676, 1048
MaeI CTAG 1 cut(s) 690
MaeII ACGT 3 cut(s) 236, 711, 1101
MaeIII GTNAC 3 cut(s) 452, 1102, 1113
MalI GATC 4 cut(s) 426, 900, 1021, 1041
MboI GATC 4 cut(s) 424, 898, 1019, 1039
MboII GAAGA 2 cut(s) 347, 608
MflI RGATCY 2 cut(s) 898, 1039
MhlI GDGCHC 1 cut(s) 778
MnlI CCTC 5 cut(s) 171, 391, 925, 965, 1074
MseI TTAA 1 cut(s) 840
MslI CAYNNNNRTG 1 cut(s) 587
Mva1269I GAATGC 1 cut(s) 495
MwoI GCNNNNNNNGC 4 cut(s) 338, 341, 835, 944
NcoI CCATGG 2 cut(s) 457, 582
NdeI CATATG 1 cut(s) 922
NdeII GATC 4 cut(s) 424, 898, 1019, 1039
NlaIII CATG 6 cut(s) 167, 461, 484, 586, 829, 951
NlaIV GGNNCC 3 cut(s) 968, 984, 1041
NmeAIII GCCGAG 1 cut(s) 995
NmuCI GTSAC 1 cut(s) 1113
NsbI TGCGCA 1 cut(s) 471
NspI RCATGY 2 cut(s) 829, 951
PaeI GCATGC 2 cut(s) 829, 951
PctI GAATGC 1 cut(s) 495
PfeI GAWTC 2 cut(s) 98, 429
PkrI GCNGC 7 cut(s) 56, 334, 343, 505, 622, 953, 1063
Ppu21I YACGTR 1 cut(s) 1102
Psp1406I AACGTT 1 cut(s) 711
PspFI CCCAGC 1 cut(s) 962
PspN4I GGNNCC 3 cut(s) 968, 984, 1041
PspPI GGNCC 1 cut(s) 796
PsuI RGATCY 2 cut(s) 898, 1039
RsaI GTAC 2 cut(s) 730, 984
RsaNI GTAC 2 cut(s) 729, 983
RseI CAYNNNNRTG 1 cut(s) 587
SaqAI TTAA 1 cut(s) 840
SatI GCNGC 7 cut(s) 55, 333, 342, 504, 621, 952, 1062
Sau3AI GATC 4 cut(s) 424, 898, 1019, 1039
Sau96I GGNCC 1 cut(s) 796
SduI GDGCHC 1 cut(s) 778
SfaNI GCATC 2 cut(s) 676, 1048
SfcI CTRYAG 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 587
SnaBI TACGTA 1 cut(s) 1102
SphI GCATGC 2 cut(s) 829, 951
SspI AATATT 1 cut(s) 891
SspMI CTAG 1 cut(s) 690
StyI CCWWGG 3 cut(s) 102, 457, 582
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 3 cut(s) 239, 714, 1104
TaqI TCGA 3 cut(s) 242, 427, 438
TfiI GAWTC 2 cut(s) 98, 429
Tru1I TTAA 1 cut(s) 840
Tru9I TTAA 1 cut(s) 840
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 1 cut(s) 1113
TseI GCWGC 7 cut(s) 54, 332, 341, 503, 620, 951, 1061
Tsp45I GTSAC 1 cut(s) 1113
TspDTI ATGAA 2 cut(s) 939, 1136
TspGWI ACGGA 1 cut(s) 1070
TspRI CASTG 1 cut(s) 412
XapI RAATTY 7 cut(s) 21, 151, 310, 393, 657, 926, 1123
XceI RCATGY 2 cut(s) 829, 951
XspI CTAG 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.