MD16G1153000.v1.1

GDSL esterase lipase At4g16230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0019746
Physical Location & Seq
Reverse (-)
12091669 .. 12093207
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1153000.v1.1.491

Sequence Viewer

Length: 690 bp
ATGAGGAGTTTATTCCAAAATTTGATAGGCTACTTGTTTTTGGTTAGCTGCTCATCAGCCAAAGATGTTCCAGCTTTTTTTACTTTCGGTGATTCCATGGTTGAGGTGGGAAACAACAACTATCTTCAAACCTTAGCAAAGGCGAATGTTTCTCCCTTTGGAATTGATTTTGGAAAGCCAACTGGACGATTTACAAATGGGAGAACAATTTTCGACATTCCTCGTATAAGAATTTTCTGTGCTGTTTTGGGTTTTAAAAATTTCACCCCTCCTTACTTGGCACCAACTACAGTTGGAGACATGCTTCTACAGGGTGTCAATTATGCTTCTTCTGCTGCTGGAATTTTCAACATTACTGGATTTTACTTTACACTCTACAACTTGGATGCTAGGAACATTATTTTCAGCAACGTCGGACTCATTGGATGTATCCCGTTTCAGAAAGGTTTACATCTAGTTGCAAAGGGAAGTTGTGCAGGAAAACTAAATCAGTTGGCGGAGAAATATAACGAGAAGTTGAAGATCCTACTCGAGAAGCTTAACATAGATCTCGAAGGGGCAAACTTTATTTATGCAGATACATACCGAATGTTACAAGATATCACCCAAAATTATGTGTCATATGGTTTGTATAAATATCCTACGTTTTTAGACCTTTCATTCACAATCGCAGCATTGTTGATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

230

Amino Acids

25.46

Weight (kDa)

8.8

Isoelectric Point (pI)

27.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 129 - 202 8.8e-07 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 280
AciI CCGC 1 cut(s) 497
AclWI GGATC 1 cut(s) 517
AcsI RAATTY 4 cut(s) 19, 231, 259, 342
AgsI TTSAA 3 cut(s) 128, 349, 520
AluBI AGCT 3 cut(s) 48, 74, 538
AluI AGCT 3 cut(s) 48, 74, 538
Alw26I GTCTC 1 cut(s) 291
AlwI GGATC 1 cut(s) 517
Ama87I CYCGRG 1 cut(s) 530
ApeKI GCWGC 3 cut(s) 48, 335, 671
ApoI RAATTY 4 cut(s) 19, 231, 259, 342
AseI ATTAAT 1 cut(s) 684
AsuHPI GGTGA 3 cut(s) 101, 256, 595
AvaI CYCGRG 1 cut(s) 530
BanI GGYRCC 1 cut(s) 280
BbvI GCAGC 3 cut(s) 35, 322, 683
BciVI GTATCC 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 291
BfaI CTAG 2 cut(s) 390, 455
BfmI CTRYAG 2 cut(s) 288, 308
BfuI GTATCC 1 cut(s) 440
BglII AGATCT 1 cut(s) 547
BisI GCNGC 3 cut(s) 49, 336, 672
BlsI GCNGC 3 cut(s) 50, 337, 673
BmeT110I CYCGRG 1 cut(s) 530
BmiI GGNNCC 1 cut(s) 282
BmsI GCATC 1 cut(s) 376
Bpu10I CCTNAGC 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 96
Bse1I ACTGG 2 cut(s) 187, 361
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 2 cut(s) 391, 431
BseNI ACTGG 2 cut(s) 187, 361
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 3 cut(s) 35, 322, 683
BsgI GTGCAG 1 cut(s) 495
BshNI GGYRCC 1 cut(s) 280
BsiHKCI CYCGRG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 291
BsoBI CYCGRG 1 cut(s) 530
Bsp143I GATC 2 cut(s) 522, 547
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 1 cut(s) 497
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 1 cut(s) 517
BspT107I GGYRCC 1 cut(s) 280
BsrI ACTGG 2 cut(s) 187, 361
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 522, 547
BssT1I CCWWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 292
BstDEI CTNAG 1 cut(s) 133
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 391, 431
BstKTI GATC 2 cut(s) 525, 550
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 2 cut(s) 522, 547
BstMWI GCNNNNNNNGC 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 304
BstSFI CTRYAG 2 cut(s) 288, 308
BstV1I GCAGC 3 cut(s) 35, 322, 683
BstX2I RGATCY 2 cut(s) 522, 547
BstYI RGATCY 2 cut(s) 522, 547
BsuI GTATCC 1 cut(s) 440
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 2 cut(s) 391, 431
CviAII CATG 2 cut(s) 97, 301
CviJI RGCY 6 cut(s) 30, 48, 59, 74, 178, 538
CviKI_1 RGCY 6 cut(s) 30, 48, 59, 74, 178, 538
DdeI CTNAG 1 cut(s) 133
DpnI GATC 2 cut(s) 524, 549
DpnII GATC 2 cut(s) 522, 547
DraI TTTAAA 1 cut(s) 256
EciI GGCGGA 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 96
Eco32I GATATC 1 cut(s) 601
Eco88I CYCGRG 1 cut(s) 530
EcoRV GATATC 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 2 cut(s) 100, 304
FatI CATG 2 cut(s) 96, 300
FauNDI CATATG 1 cut(s) 622
Fnu4HI GCNGC 3 cut(s) 49, 336, 672
FokI GGATG 2 cut(s) 398, 438
Fsp4HI GCNGC 3 cut(s) 49, 336, 672
FspBI CTAG 2 cut(s) 390, 455
GluI GCNGC 3 cut(s) 49, 336, 672
Hin1II CATG 2 cut(s) 100, 304
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 2 cut(s) 92, 417
HphI GGTGA 3 cut(s) 101, 256, 595
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 2 cut(s) 416, 441
Hpy188III TCNNGA 2 cut(s) 532, 551
Hpy8I GTNNAC 1 cut(s) 449
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 1 cut(s) 548
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 2 cut(s) 411, 644
HpyCH4V TGCA 3 cut(s) 461, 476, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 332
HpyF3I CTNAG 1 cut(s) 133
HpySE526I ACGT 2 cut(s) 411, 644
Hsp92II CATG 2 cut(s) 100, 304
Kzo9I GATC 2 cut(s) 522, 547
LpnPI CCDG 6 cut(s) 84, 168, 296, 324, 342, 462
Lsp1109I GCAGC 3 cut(s) 35, 322, 683
LweI GCATC 1 cut(s) 376
MaeI CTAG 2 cut(s) 390, 455
MaeII ACGT 2 cut(s) 411, 644
MaeIII GTNAC 1 cut(s) 591
MalI GATC 2 cut(s) 524, 549
MboI GATC 2 cut(s) 522, 547
MboII GAAGA 3 cut(s) 116, 321, 532
MflI RGATCY 2 cut(s) 522, 547
MluCI AATT 9 cut(s) 19, 162, 207, 231, 259, 319, 342, 610, 685
MlyI GAGTC 1 cut(s) 411
MmeI TCCRAC 2 cut(s) 274, 394
MnlI CCTC 3 cut(s) 97, 231, 279
MseI TTAA 4 cut(s) 255, 540, 684, 688
MwoI GCNNNNNNNGC 1 cut(s) 332
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 622
NdeII GATC 2 cut(s) 522, 547
NlaIII CATG 2 cut(s) 100, 304
NlaIV GGNNCC 1 cut(s) 282
NspI RCATGY 1 cut(s) 304
PacI TTAATTAA 1 cut(s) 688
PaeR7I CTCGAG 1 cut(s) 530
PfeI GAWTC 1 cut(s) 92
PkrI GCNGC 3 cut(s) 50, 337, 673
PleI GAGTC 1 cut(s) 411
PpsI GAGTC 1 cut(s) 411
PshBI ATTAAT 1 cut(s) 684
PspN4I GGNNCC 1 cut(s) 282
PsuI RGATCY 2 cut(s) 522, 547
SaqAI TTAA 4 cut(s) 255, 540, 684, 688
SatI GCNGC 3 cut(s) 49, 336, 672
Sau3AI GATC 2 cut(s) 522, 547
SchI GAGTC 1 cut(s) 411
SetI ASST 9 cut(s) 50, 76, 108, 134, 414, 448, 540, 647, 657
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 2 cut(s) 288, 308
Sfr274I CTCGAG 1 cut(s) 530
SlaI CTCGAG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 9 cut(s) 19, 162, 207, 231, 259, 319, 342, 610, 685
SsiI CCGC 1 cut(s) 497
SspMI CTAG 2 cut(s) 390, 455
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 2 cut(s) 414, 647
TaqI TCGA 3 cut(s) 213, 531, 552
TasI AATT 9 cut(s) 19, 162, 207, 231, 259, 319, 342, 610, 685
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 4 cut(s) 255, 540, 684, 688
Tru9I TTAA 4 cut(s) 255, 540, 684, 688
TseI GCWGC 3 cut(s) 48, 335, 671
TspDTI ATGAA 1 cut(s) 648
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 4 cut(s) 19, 231, 259, 342
XceI RCATGY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 2 cut(s) 390, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.