Prupe.1G116400_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0019746
Physical Location & Seq
Reverse (-)
9168800 .. 9170850
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G116400.1

Sequence Viewer

Length: 1107 bp
ATGAAAAAGAAAGAAAGGTTATCATTATTTCATGAAATGGGGATTATATTCCAAATTCTTGTAGTCTACTTCTTCTTGTTGGTTAGCTGCTCAGCCAACAAAAGTGTTCCTGCTTTTTTCACTTTTGGCGATTCCTTGGTTGATGTGGGGAACAACAACAACCTTCTAACTCAAGCTAAGGCAAATTTTTACCCTCATGGAATTGATTTTGGGAATAACCCAAGAGGACAATTTTCAAATGGGAGAACAACTTTTGACATTCTTCAACAGGAATTGGGTTTCGCACAATTTACTCCTCCTTACTTGGCACCAACTACTGTTGGAGACGCACTTCTGCAGGGTGTCAATTATGCTTCCTCTGGCTCTGGAATTTTCAATTATACTGGATATTTCTTTGGTGAACGAATCAATTTTGACACGCAAATAAGTAACCATGGAAGAACAGTGCTTGCTATCATGTCGAGGATTGGCATTCCTGCAGCTCAGAAATTGTTGAGAAGAGCCATTTATGGGGTTGTAATTGGCTCTAACGATATAATGTTCAGGGAGTTTACACCCATGGCCATGTCCATGTCCGAAGAAGCTTATTTGGATTTCTTGATTTCAAGACTCAAATTAGCACTCACTACACTCTACAACTTGAATGCTAGAAAGATCACCGTCGCCAACGCTGGACCTAGTGGGTGTATTCCATATGAGAAAGAAATACATCCAGTTCCAAAGGGAAGTTGTGTTCGTCTGATAAATCGTATGGCTCAAACATATAACGACAAGTTGAAGGGCATGCTTGCCGAGCTTAACAAAGATCTTCAAGGGGCAAAGTTTATTTACGTAGATATTTACCAAATATTACTAGATCTCACAAAAAATTATGTGTCATATGGCTTTGAGAATGCAACTTCTGCATGCTGCTCGTTTGCCGTGACCCGAGTGGGACTGGTGCCATGCAATCCTTTTTCTAAAATTTGTCCCGACAGATCCAAGTATATATTCTGGGATGCGTTTCACTTAACAGACGCTGCTAATGTTATTGCAACAAGGCATATTATGGATGGTGGCTTAAATTATGTATCACCTATGAATTTTAGGCAACTTGTTCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

41.08

Weight (kDa)

8.94

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 307, 940
AccI GTMKAC 1 cut(s) 66
AclWI GGATC 1 cut(s) 972
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 5 cut(s) 54, 184, 369, 963, 1081
AfiI CCNNNNNNNGG 1 cut(s) 510
AgsI TTSAA 7 cut(s) 237, 266, 376, 606, 643, 778, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 974, 1006
AloI GAACNNNNNNTCC 2 cut(s) 717, 749
AluBI AGCT 5 cut(s) 87, 176, 482, 584, 796
AluI AGCT 5 cut(s) 87, 176, 482, 584, 796
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 1 cut(s) 972
AlwNI CAGNNNCTG 1 cut(s) 1019
Ama87I CYCGRG 1 cut(s) 927
AoxI GGCC 1 cut(s) 561
ApeKI GCWGC 4 cut(s) 87, 479, 909, 1019
ApoI RAATTY 5 cut(s) 54, 184, 369, 963, 1081
AspS9I GGNCC 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 410, 649, 1065
AvaI CYCGRG 1 cut(s) 927
AvaII GGWCC 1 cut(s) 674
BalI TGGCCA 1 cut(s) 563
BanI GGYRCC 2 cut(s) 307, 940
BbvI GCAGC 4 cut(s) 74, 491, 896, 1006
BccI CCATC 1 cut(s) 1046
BceAI ACGGC 1 cut(s) 905
BcgI CGANNNNNNTGC 2 cut(s) 894, 928
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 3 cut(s) 648, 678, 854
BfmI CTRYAG 2 cut(s) 335, 477
BglII AGATCT 2 cut(s) 805, 856
BisI GCNGC 4 cut(s) 88, 480, 910, 1020
BlpI GCTNAGC 1 cut(s) 91
BlsI GCNGC 4 cut(s) 89, 481, 911, 1021
Bme18I GGWCC 1 cut(s) 674
BmeT110I CYCGRG 1 cut(s) 927
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 2 cut(s) 309, 942
BmsI GCATC 1 cut(s) 988
Bpu10I CCTNAGC 1 cut(s) 177
Bpu1102I GCTNAGC 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 832
BsaJI CCNNGG 3 cut(s) 135, 433, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse1I ACTGG 3 cut(s) 388, 713, 942
BseDI CCNNGG 3 cut(s) 135, 433, 558
BseGI GGATG 3 cut(s) 709, 1003, 1057
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 105, 497
BseNI ACTGG 3 cut(s) 388, 713, 942
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 4 cut(s) 74, 491, 896, 1006
BshFI GGCC 1 cut(s) 563
BshNI GGYRCC 2 cut(s) 307, 940
BsiHKCI CYCGRG 1 cut(s) 927
BslFI GGGAC 2 cut(s) 948, 954
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 1 cut(s) 318
BsmBI CGTCTC 1 cut(s) 318
BsmFI GGGAC 2 cut(s) 948, 954
BsmI GAATGC 3 cut(s) 471, 649, 898
BsnI GGCC 1 cut(s) 563
BsoBI CYCGRG 1 cut(s) 927
Bsp143I GATC 4 cut(s) 654, 805, 856, 977
Bsp1720I GCTNAGC 1 cut(s) 91
Bsp19I CCATGG 2 cut(s) 433, 558
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 2 cut(s) 104, 496
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 309, 942
BspMAI CTGCAG 2 cut(s) 339, 481
BspPI GGATC 1 cut(s) 972
BspQI GCTCTTC 1 cut(s) 493
BspT107I GGYRCC 2 cut(s) 307, 940
BsrI ACTGG 3 cut(s) 388, 713, 942
BssECI CCNNGG 3 cut(s) 135, 433, 558
BssMI GATC 4 cut(s) 654, 805, 856, 977
BssT1I CCWWGG 3 cut(s) 135, 433, 558
Bst4CI ACNGT 3 cut(s) 319, 445, 661
Bst6I CTCTTC 1 cut(s) 493
BstAPI GCANNNNNTGC 1 cut(s) 902
BstBAI YACGTR 1 cut(s) 832
BstC8I GCNNGC 4 cut(s) 450, 785, 789, 907
BstDEI CTNAG 3 cut(s) 91, 177, 483
BstDSI CCRYGG 2 cut(s) 433, 558
BstF5I GGATG 3 cut(s) 709, 1003, 1057
BstKTI GATC 4 cut(s) 657, 808, 859, 980
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 4 cut(s) 654, 805, 856, 977
BstMWI GCNNNNNNNGC 2 cut(s) 793, 902
BstNSI RCATGY 2 cut(s) 787, 909
BstSFI CTRYAG 2 cut(s) 335, 477
BstSNI TACGTA 1 cut(s) 832
BstV1I GCAGC 4 cut(s) 74, 491, 896, 1006
BstX2I RGATCY 3 cut(s) 805, 856, 977
BstYI RGATCY 3 cut(s) 805, 856, 977
BsuRI GGCC 1 cut(s) 563
BtgI CCRYGG 2 cut(s) 433, 558
BtsCI GGATG 3 cut(s) 709, 1003, 1057
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 4 cut(s) 450, 785, 789, 907
CaiI CAGNNNCTG 1 cut(s) 1019
CciI TCATGA 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 674
CseI GACGC 2 cut(s) 335, 1025
DdeI CTNAG 3 cut(s) 91, 177, 483
DpnI GATC 4 cut(s) 656, 807, 858, 979
DpnII GATC 4 cut(s) 654, 805, 856, 977
EaeI YGGCCR 1 cut(s) 561
Eam1104I CTCTTC 1 cut(s) 493
EarI CTCTTC 1 cut(s) 493
Eco105I TACGTA 1 cut(s) 832
Eco130I CCWWGG 3 cut(s) 135, 433, 558
Eco47I GGWCC 1 cut(s) 674
Eco88I CYCGRG 1 cut(s) 927
EcoT14I CCWWGG 3 cut(s) 135, 433, 558
ErhI CCWWGG 3 cut(s) 135, 433, 558
Esp3I CGTCTC 1 cut(s) 318
FaqI GGGAC 2 cut(s) 948, 954
FauNDI CATATG 2 cut(s) 694, 880
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 4 cut(s) 88, 480, 910, 1020
FokI GGATG 3 cut(s) 696, 1010, 1064
Fsp4HI GCNGC 4 cut(s) 88, 480, 910, 1020
FspBI CTAG 3 cut(s) 648, 678, 854
GluI GCNGC 4 cut(s) 88, 480, 910, 1020
HaeIII GGCC 1 cut(s) 563
HgaI GACGC 2 cut(s) 335, 1025
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 3 cut(s) 131, 405, 609
HphI GGTGA 3 cut(s) 410, 649, 1065
Hpy166II GTNNAC 3 cut(s) 67, 401, 552
Hpy188I TCNGA 3 cut(s) 486, 577, 741
Hpy188III TCNNGA 6 cut(s) 32, 366, 598, 606, 971, 1104
Hpy8I GTNNAC 3 cut(s) 67, 401, 552
Hpy99I CGWCG 1 cut(s) 665
HpyAV CCTTC 2 cut(s) 173, 772
HpyCH4III ACNGT 3 cut(s) 319, 445, 661
HpyCH4IV ACGT 1 cut(s) 831
HpyCH4V TGCA 6 cut(s) 337, 479, 896, 905, 948, 1034
HpyF10VI GCNNNNNNNGC 2 cut(s) 793, 902
HpyF3I CTNAG 3 cut(s) 91, 177, 483
HpySE526I ACGT 1 cut(s) 831
Kzo9I GATC 4 cut(s) 654, 805, 856, 977
LguI GCTCTTC 1 cut(s) 493
Lsp1109I GCAGC 4 cut(s) 74, 491, 896, 1006
LweI GCATC 1 cut(s) 988
MaeI CTAG 3 cut(s) 648, 678, 854
MaeII ACGT 1 cut(s) 831
MaeIII GTNAC 2 cut(s) 428, 922
MalI GATC 4 cut(s) 656, 807, 858, 979
MboI GATC 4 cut(s) 654, 805, 856, 977
MboII GAAGA 6 cut(s) 64, 254, 450, 510, 590, 800
MflI RGATCY 3 cut(s) 805, 856, 977
MlsI TGGCCA 1 cut(s) 563
MluNI TGGCCA 1 cut(s) 563
MlyI GAGTC 1 cut(s) 603
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 5 cut(s) 204, 218, 306, 367, 456
Mox20I TGGCCA 1 cut(s) 563
MscI TGGCCA 1 cut(s) 563
MseI TTAA 3 cut(s) 798, 1010, 1061
MslI CAYNNNNRTG 2 cut(s) 563, 569
Msp20I TGGCCA 1 cut(s) 563
Mva1269I GAATGC 3 cut(s) 471, 649, 898
MwoI GCNNNNNNNGC 2 cut(s) 793, 902
NcoI CCATGG 2 cut(s) 433, 558
NdeI CATATG 2 cut(s) 694, 880
NdeII GATC 4 cut(s) 654, 805, 856, 977
NlaIV GGNNCC 2 cut(s) 309, 942
NmeAIII GCCGAG 1 cut(s) 817
NmuCI GTSAC 1 cut(s) 922
NspI RCATGY 2 cut(s) 787, 909
PaeI GCATGC 2 cut(s) 787, 909
PagI TCATGA 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 493
PctI GAATGC 3 cut(s) 471, 649, 898
PfeI GAWTC 2 cut(s) 131, 405
PkrI GCNGC 4 cut(s) 89, 481, 911, 1021
PleI GAGTC 1 cut(s) 603
PpsI GAGTC 1 cut(s) 603
Ppu21I YACGTR 1 cut(s) 832
PspN4I GGNNCC 2 cut(s) 309, 942
PspPI GGNCC 1 cut(s) 674
PstI CTGCAG 2 cut(s) 339, 481
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 3 cut(s) 805, 856, 977
RseI CAYNNNNRTG 2 cut(s) 563, 569
SapI GCTCTTC 1 cut(s) 493
SaqAI TTAA 3 cut(s) 798, 1010, 1061
SatI GCNGC 4 cut(s) 88, 480, 910, 1020
Sau3AI GATC 4 cut(s) 654, 805, 856, 977
Sau96I GGNCC 1 cut(s) 674
SchI GAGTC 1 cut(s) 603
SfaNI GCATC 1 cut(s) 988
SfcI CTRYAG 2 cut(s) 335, 477
SinI GGWCC 1 cut(s) 674
SmiMI CAYNNNNRTG 2 cut(s) 563, 569
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
SnaBI TACGTA 1 cut(s) 832
SphI GCATGC 2 cut(s) 787, 909
SspI AATATT 1 cut(s) 849
SspMI CTAG 3 cut(s) 648, 678, 854
StyI CCWWGG 3 cut(s) 135, 433, 558
TaaI ACNGT 3 cut(s) 319, 445, 661
TaiI ACGT 1 cut(s) 834
TaqI TCGA 1 cut(s) 461
TfiI GAWTC 2 cut(s) 131, 405
Tru1I TTAA 3 cut(s) 798, 1010, 1061
Tru9I TTAA 3 cut(s) 798, 1010, 1061
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 1 cut(s) 922
TseI GCWGC 4 cut(s) 87, 479, 909, 1019
Tsp45I GTSAC 1 cut(s) 922
TspDTI ATGAA 4 cut(s) 17, 20, 48, 1094
TspRI CASTG 1 cut(s) 450
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 5 cut(s) 54, 184, 369, 963, 1081
XceI RCATGY 2 cut(s) 787, 909
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 3 cut(s) 648, 678, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.