MD16G1153100.v1.1

GDSL esterase lipase At4g16230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0019746
Physical Location & Seq
Forward (+)
12112901 .. 12117101
4201 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1153100.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGGGAATATTAATATTACGTTCCATATTTGCGTAGTCTACTTGTTTGTGATGACTTGCTCATCTGCCCAAAGTGTTCCTGCTTTTTTCATCTTTGGCGATTCTGCGGCAGATGTGGGAAATAACAACTACGTTGCAACCGCAGCAAAGGCGAATACCATTCCCAATGGAATCGATTTTGGGAATCCAACAGGACGATTTACAAATGGCAGAACTGTTTTTGACATTCTTCAACAGCAAGTGGGTTTCAAAAATTTCTCTCCTGCTTACTTAGCTCCAACTACCGTTGGAGACGTGATTCTACAAGGTGTCAATTATGCTGCATCTGCCTCTGGAATTTTTAATTCCACTGGATCACAATATGGTGAAACAGTCAGTTTCGACACACAACTCAAATATCATGGGAGAATTACGCTTGCCATAATGTCAAGAATTGGTGTTGCTGCAACTCAGAAATTGTTGAGGAAATCAATCTATGCAATTGCACTAGGCGCTAACGATATACTGTCTAAGGATTCGTACACATCCAAGCTAACGCAAGACGAGTTTTTGGATTTCGTGATCTCAAGATTCAGGTCACAACTTCAGACACTCTACAACTTGGCTGCTAGAAAGATTATGGTCAACAGTGTTGGACTCATTGGCTGTTCTCCGTTTGTTAAAGATATATTTTTTGTTGCCGAAGGCAAGTGTTATGATCCATTCAACCAATTGGCCCAGATGTATAACAAGAAGTTGAAGAGTCTGATCGTGGAGCTTAACAAAGATTTGGATGGTGCAAAATTTGTTTACGTGGATACGTACAAAACAACGGAAGATATCATCCAAAAATTTGTGTCATATGGTTTTGTGGACGCAACTTCTGCATGCTGCTCTTTTTTCGGGCGCCATGGAGGACTTTTCGGCTGCAGTCCATTTTCAAAAGTTTGTCCTGACAGATCAAAGTATGTATTTTGGGATTCATTTCACTTAACAGATGCTGCTAACCTAGTCACAGCAAAGTATATGATGGAAGGAAACTTGACATATATGTTCCCGATGAATGTTCGACAACTTGTGCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

356

Amino Acids

39.19

Weight (kDa)

8.65

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 335 5.5e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 885
AccI GTMKAC 1 cut(s) 39
AciI CCGC 2 cut(s) 107, 141
AclWI GGATC 2 cut(s) 361, 692
AcsI RAATTY 4 cut(s) 253, 336, 782, 830
AcuI CTGAAG 1 cut(s) 569
AcyI GRCGYC 1 cut(s) 886
AfaI GTAC 2 cut(s) 521, 803
AgsI TTSAA 5 cut(s) 233, 250, 706, 739, 921
AjiI CACGTC 1 cut(s) 295
AluBI AGCT 3 cut(s) 275, 532, 757
AluI AGCT 3 cut(s) 275, 532, 757
Alw26I GTCTC 1 cut(s) 285
AlwI GGATC 2 cut(s) 361, 692
AlwNI CAGNNNCTG 1 cut(s) 980
AoxI GGCC 1 cut(s) 714
ApeKI GCWGC 7 cut(s) 143, 320, 443, 605, 870, 906, 980
ApoI RAATTY 4 cut(s) 253, 336, 782, 830
AseI ATTAAT 1 cut(s) 12
AspLEI GCGC 2 cut(s) 494, 888
AspS9I GGNCC 1 cut(s) 715
AsuHPI GGTGA 1 cut(s) 377
BanI GGYRCC 1 cut(s) 885
BbvI GCAGC 7 cut(s) 155, 307, 430, 592, 857, 893, 967
BccI CCATC 2 cut(s) 767, 1003
BciVI GTATCC 1 cut(s) 790
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 3 cut(s) 488, 609, 989
BfmI CTRYAG 1 cut(s) 907
BfoI RGCGCY 2 cut(s) 495, 889
BfuI GTATCC 1 cut(s) 790
BisI GCNGC 8 cut(s) 108, 144, 321, 444, 606, 871, 907, 981
BlsI GCNGC 8 cut(s) 109, 145, 322, 445, 607, 872, 908, 982
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 715
BmiI GGNNCC 1 cut(s) 887
BmsI GCATC 2 cut(s) 332, 967
BpuEI CTTGAG 1 cut(s) 550
Bsa29I ATCGAT 1 cut(s) 174
BsaAI YACGTR 2 cut(s) 793, 801
BsaHI GRCGYC 1 cut(s) 886
BsaJI CCNNGG 1 cut(s) 889
Bse1I ACTGG 1 cut(s) 355
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 889
BseGI GGATG 3 cut(s) 524, 778, 822
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 1 cut(s) 355
BseXI GCAGC 7 cut(s) 155, 307, 430, 592, 857, 893, 967
BshFI GGCC 1 cut(s) 716
BshNI GGYRCC 1 cut(s) 885
BshVI ATCGAT 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 285
BsmBI CGTCTC 1 cut(s) 285
BsnI GGCC 1 cut(s) 716
Bsp143I GATC 5 cut(s) 353, 561, 697, 747, 938
Bsp19I CCATGG 1 cut(s) 889
BspACI CCGC 2 cut(s) 107, 141
BspANI GGCC 1 cut(s) 716
BspCNI CTCAG 1 cut(s) 463
BspDI ATCGAT 1 cut(s) 174
BspLI GGNNCC 1 cut(s) 887
BspMAI CTGCAG 1 cut(s) 911
BspPI GGATC 2 cut(s) 361, 692
BspT107I GGYRCC 1 cut(s) 885
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 889
BssMI GATC 5 cut(s) 353, 561, 697, 747, 938
BssNI GRCGYC 1 cut(s) 886
BssT1I CCWWGG 1 cut(s) 889
Bst4CI ACNGT 5 cut(s) 217, 286, 373, 507, 629
Bst6I CTCTTC 1 cut(s) 734
BstACI GRCGYC 1 cut(s) 886
BstAPI GCANNNNNTGC 1 cut(s) 863
BstBAI YACGTR 2 cut(s) 793, 801
BstC8I GCNNGC 2 cut(s) 417, 868
BstDEI CTNAG 3 cut(s) 271, 450, 510
BstDSI CCRYGG 1 cut(s) 889
BstF5I GGATG 3 cut(s) 524, 778, 822
BstH2I RGCGCY 2 cut(s) 495, 889
BstHHI GCGC 2 cut(s) 494, 888
BstKTI GATC 5 cut(s) 356, 564, 700, 750, 941
BstMAI GTCTC 1 cut(s) 285
BstMBI GATC 5 cut(s) 353, 561, 697, 747, 938
BstMWI GCNNNNNNNGC 6 cut(s) 143, 149, 272, 326, 491, 863
BstNSI RCATGY 1 cut(s) 870
BstSFI CTRYAG 1 cut(s) 907
BstSNI TACGTA 1 cut(s) 801
BstV1I GCAGC 7 cut(s) 155, 307, 430, 592, 857, 893, 967
Bsu15I ATCGAT 1 cut(s) 174
BsuI GTATCC 1 cut(s) 790
BsuRI GGCC 1 cut(s) 716
BsuTUI ATCGAT 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 889
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 3 cut(s) 524, 778, 822
BtsIMutI CAGTG 2 cut(s) 348, 634
Cac8I GCNNGC 2 cut(s) 417, 868
CaiI CAGNNNCTG 1 cut(s) 980
CfoI GCGC 2 cut(s) 494, 888
Cfr13I GGNCC 1 cut(s) 715
ClaI ATCGAT 1 cut(s) 174
CseI GACGC 1 cut(s) 863
Csp6I GTAC 2 cut(s) 520, 802
CviAII CATG 3 cut(s) 401, 867, 890
CviJI RGCY 7 cut(s) 275, 532, 605, 645, 716, 757, 906
CviKI_1 RGCY 7 cut(s) 275, 532, 605, 645, 716, 757, 906
CviQI GTAC 2 cut(s) 520, 802
DdeI CTNAG 3 cut(s) 271, 450, 510
DinI GGCGCC 1 cut(s) 887
DpnI GATC 5 cut(s) 355, 563, 699, 749, 940
DpnII GATC 5 cut(s) 353, 561, 697, 747, 938
Eam1104I CTCTTC 1 cut(s) 734
EarI CTCTTC 1 cut(s) 734
Eco105I TACGTA 1 cut(s) 801
Eco130I CCWWGG 1 cut(s) 889
Eco32I GATATC 1 cut(s) 820
Eco57I CTGAAG 1 cut(s) 569
EcoRV GATATC 1 cut(s) 820
EcoT14I CCWWGG 1 cut(s) 889
EgeI GGCGCC 1 cut(s) 887
EheI GGCGCC 1 cut(s) 887
ErhI CCWWGG 1 cut(s) 889
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 3 cut(s) 404, 870, 893
FatI CATG 3 cut(s) 400, 866, 889
FauNDI CATATG 1 cut(s) 841
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 8 cut(s) 108, 144, 321, 444, 606, 871, 907, 981
FokI GGATG 3 cut(s) 511, 785, 809
Fsp4HI GCNGC 8 cut(s) 108, 144, 321, 444, 606, 871, 907, 981
FspBI CTAG 3 cut(s) 488, 609, 989
GlaI GCGC 2 cut(s) 493, 887
GluI GCNGC 8 cut(s) 108, 144, 321, 444, 606, 871, 907, 981
HaeII RGCGCY 2 cut(s) 495, 889
HaeIII GGCC 1 cut(s) 716
HgaI GACGC 1 cut(s) 863
HhaI GCGC 2 cut(s) 494, 888
Hin1I GRCGYC 1 cut(s) 886
Hin1II CATG 3 cut(s) 404, 870, 893
Hin6I GCGC 2 cut(s) 492, 886
HinP1I GCGC 2 cut(s) 492, 886
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 9 cut(s) 101, 171, 184, 298, 515, 570, 636, 742, 959
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 5 cut(s) 40, 522, 625, 790, 853
Hpy188I TCNGA 3 cut(s) 453, 588, 747
Hpy188III TCNNGA 7 cut(s) 333, 429, 559, 567, 932, 1036, 1065
Hpy8I GTNNAC 5 cut(s) 40, 522, 625, 790, 853
HpyAV CCTTC 2 cut(s) 677, 1007
HpyCH4III ACNGT 5 cut(s) 217, 286, 373, 507, 629
HpyCH4IV ACGT 5 cut(s) 20, 132, 294, 792, 800
HpyCH4V TGCA 9 cut(s) 137, 323, 446, 479, 485, 779, 866, 909, 1060
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 149, 272, 326, 491, 863
HpyF3I CTNAG 3 cut(s) 271, 450, 510
HpySE526I ACGT 5 cut(s) 20, 132, 294, 792, 800
Hsp92I GRCGYC 1 cut(s) 886
Hsp92II CATG 3 cut(s) 404, 870, 893
HspAI GCGC 2 cut(s) 492, 886
KasI GGCGCC 1 cut(s) 885
Kzo9I GATC 5 cut(s) 353, 561, 697, 747, 938
LmnI GCTCC 2 cut(s) 280, 754
LpnPI CCDG 8 cut(s) 93, 177, 276, 318, 336, 559, 731, 945
Lsp1109I GCAGC 7 cut(s) 155, 307, 430, 592, 857, 893, 967
LweI GCATC 2 cut(s) 332, 967
MaeI CTAG 3 cut(s) 488, 609, 989
MaeII ACGT 5 cut(s) 20, 132, 294, 792, 800
MaeIII GTNAC 2 cut(s) 576, 991
MalI GATC 5 cut(s) 355, 563, 699, 749, 940
MboI GATC 5 cut(s) 353, 561, 697, 747, 938
MboII GAAGA 3 cut(s) 221, 751, 827
MfeI CAATTG 2 cut(s) 480, 710
Mly113I GGCGCC 1 cut(s) 886
MlyI GAGTC 2 cut(s) 630, 751
MmeI TCCRAC 4 cut(s) 212, 268, 302, 613
MnlI CCTC 3 cut(s) 340, 456, 887
MseI TTAA 5 cut(s) 12, 342, 660, 759, 971
MunI CAATTG 2 cut(s) 480, 710
MwoI GCNNNNNNNGC 6 cut(s) 143, 149, 272, 326, 491, 863
NarI GGCGCC 1 cut(s) 886
NcoI CCATGG 1 cut(s) 889
NdeI CATATG 1 cut(s) 841
NdeII GATC 5 cut(s) 353, 561, 697, 747, 938
NlaIII CATG 3 cut(s) 404, 870, 893
NlaIV GGNNCC 1 cut(s) 887
NmuCI GTSAC 2 cut(s) 576, 991
NspI RCATGY 1 cut(s) 870
PaeI GCATGC 1 cut(s) 870
PfeI GAWTC 7 cut(s) 101, 171, 184, 298, 515, 570, 959
PkrI GCNGC 8 cut(s) 109, 145, 322, 445, 607, 872, 908, 982
PleI GAGTC 2 cut(s) 630, 750
PluTI GGCGCC 1 cut(s) 889
PpsI GAGTC 2 cut(s) 630, 750
Ppu21I YACGTR 2 cut(s) 793, 801
PshBI ATTAAT 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 887
PspPI GGNCC 1 cut(s) 715
PstI CTGCAG 1 cut(s) 911
PstNI CAGNNNCTG 1 cut(s) 980
RsaI GTAC 2 cut(s) 521, 803
RsaNI GTAC 2 cut(s) 520, 802
SaqAI TTAA 5 cut(s) 12, 342, 660, 759, 971
SatI GCNGC 8 cut(s) 108, 144, 321, 444, 606, 871, 907, 981
Sau3AI GATC 5 cut(s) 353, 561, 697, 747, 938
Sau96I GGNCC 1 cut(s) 715
SchI GAGTC 2 cut(s) 630, 751
SfaNI GCATC 2 cut(s) 332, 967
SfcI CTRYAG 1 cut(s) 907
SfoI GGCGCC 1 cut(s) 887
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
SnaBI TACGTA 1 cut(s) 801
SphI GCATGC 1 cut(s) 870
SsiI CCGC 2 cut(s) 107, 141
SspDI GGCGCC 1 cut(s) 885
SspI AATATT 2 cut(s) 10, 16
SspMI CTAG 3 cut(s) 488, 609, 989
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 5 cut(s) 217, 286, 373, 507, 629
TaiI ACGT 5 cut(s) 23, 135, 297, 795, 803
TaqI TCGA 3 cut(s) 174, 381, 1048
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 7 cut(s) 101, 171, 184, 298, 515, 570, 959
Tru1I TTAA 5 cut(s) 12, 342, 660, 759, 971
Tru9I TTAA 5 cut(s) 12, 342, 660, 759, 971
TscAI CASTG 2 cut(s) 355, 634
TseFI GTSAC 2 cut(s) 576, 991
TseI GCWGC 7 cut(s) 143, 320, 443, 605, 870, 906, 980
Tsp45I GTSAC 2 cut(s) 576, 991
TspDTI ATGAA 3 cut(s) 79, 951, 1055
TspGWI ACGGA 2 cut(s) 642, 827
TspRI CASTG 2 cut(s) 355, 634
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 4 cut(s) 253, 336, 782, 830
XceI RCATGY 1 cut(s) 870
XmiI GTMKAC 1 cut(s) 39
XspI CTAG 3 cut(s) 488, 609, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.