MD13G1193100.v1.1

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010357
Physical Location & Seq
Forward (+)
16726724 .. 16730710
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1193100.v1.1.491

Sequence Viewer

Length: 1569 bp
ATGGGGTCGATCCCCGATCCCGGCGAGTTGACCGAGTCGACTCCGCCGAGCTTCGACGAGTTCCAGCGGCAGACCTCTCTCATGACCAGCTGCACTCTCCTCTGGAAGGAGCTCTCCGACCACTTCTCGTCCCTCGAGCAGAACCTCCTGAAGAAATCCGAGGCCATCAAGCTCAAAATCCAGACCCTGGAGCACGAGACCAAGGCCTCGCTCGACGAGCTCGAGCAGCGCGAGGTCTCGATCAGGGACATCTTCGAAGTCGCGCTTGGCAAGGTTGAGAAGAGCAAGGAGGCGGCGCTCAAGGTGTTGAAGAGAGCCCGCAGCGACGACGAGTACGGGGAGGTGGACGACGGCGAGGGGTTGCTGATGAATCTCAAGTCGTTGTGCTTGAAGATGGATTCTGGAGGGTTTTGGAGGTTCGTGACGGCGAGGAAGAAGGAATTGGAGGCTCTGAGGTCGCAGATGCCTCTGGCTTTGGGCGATTGTGTCGATCCGGCGAAGTTCGTGCTAGAGGCGATATCGGAGGTGTTCCCGGTGGACAAGAGAGTGGAGAGGAGCGATAGGGGAAACGATTTGGGCTGGGCCTGCGTTCTGTTGCTCGAGTCACTGATTCCGGTGATGATGGACCCGAAGATCGGGAGAAAGAGGCTTCTGGTGACGCGGAGCGTGAAGCAACGCGCCATCGAGATATCCGAGACCTGGAAGGCCAGCTTGGAGGAGAGAGGCGGCATAGAGAACGTGAAGACTCCTGATGTGCACACGTTCCTGCAGCATTTGGTGACTTTCGGGATTGTGAAGGAGGAGGATGTTGAGTTGTACAGGAAGCTTGTGGTTAGCTCAGCCTGGCGCAAGCAGATGCCGAAGCTCGCTGTTTCGCTTGGCCTCGCCAAGCAAATGCCGGATATGATTGAAGAATTGATCAGCAGGGGACAGCAGCTTGATGCGGTGCATTTCACCTATGGGGTTGGACTTGTGCACAAGTTTCCTCCTGTTCCTCTACTGAAAGCTTTTCTGAAGGATGCTAAGAAAGCTGCGGCTTCTATTTTGGAAGATCCCAATAACGCTGGGCGAGCTGCGAACCTAGCTGGACGCAAAGAGCTGTCAGCGGTCCGGGCTGTCATCAAGTGCATTGAAGATTATAACCTTGAGGCAGAATTTCCTCCAGAAGACCTCAAGAAGCGCCTTGAACAGCTAGAGAAGGTGAAACCAGAGAAGAAAAGGCCAGTTGTAGTACCAGCCAACAAACGAGTACGAGCTAACAATGGCGGTCCCATGCCTCCGGCCAAGGCTGGCCGCTTGACGAATGCATATGTGTCTTCGTTCCCTACACCTCCTACATTTGTCAGGTCTCCTTCCCATGGCCAGTATCCTGCTGGGTTCTCACCGTACCATTCCCCACCCACCATGTATGGTAGCAGAAGCCCACCAACCCCTTATGCATACTCACCGGAAGCAGCACCCCCTCCTCCTCATGCCGGATCATACCCTGGACCTCCGATGAACTATCCTGCATATGGGGCTTATGGCAATGGTATGGTACCAGCTTATCAGCCGGCTTACTACCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

523

Amino Acids

58.07

Weight (kDa)

8.65

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 123 - 408 9.9e-104 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
Acc65I GGTACC 1 cut(s) 1537
AccB1I GGYRCC 1 cut(s) 1537
AccB7I CCANNNNNTGG 1 cut(s) 187
AccI GTMKAC 1 cut(s) 38
AccII CGCG 4 cut(s) 231, 263, 661, 678
AclWI GGATC 5 cut(s) 4, 11, 485, 1046, 1486
AcoI YGGCCR 3 cut(s) 1281, 1291, 1360
AcsI RAATTY 1 cut(s) 1154
AcuI CTGAAG 2 cut(s) 170, 1034
AfaI GTAC 6 cut(s) 335, 818, 1233, 1251, 1388, 1539
AfiI CCNNNNNNNGG 8 cut(s) 20, 106, 187, 635, 699, 1358, 1475, 1514
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 5 cut(s) 310, 391, 911, 1133, 1187
AjnI CCWGG 4 cut(s) 186, 698, 842, 1486
AjuI GAANNNNNNNTTGG 6 cut(s) 249, 281, 425, 457, 695, 727
Alw21I GWGCWC 5 cut(s) 114, 195, 222, 759, 978
Alw26I GTCTC 4 cut(s) 191, 241, 689, 1353
Alw44I GTGCAC 2 cut(s) 755, 974
AlwI GGATC 5 cut(s) 4, 11, 485, 1046, 1486
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 3 cut(s) 134, 221, 599
AoxI GGCC 9 cut(s) 162, 204, 582, 705, 880, 1220, 1281, 1291, 1360
ApaLI GTGCAC 2 cut(s) 755, 974
ApeKI GCWGC 8 cut(s) 90, 226, 321, 769, 934, 1031, 1073, 1454
ApoI RAATTY 1 cut(s) 1154
Asp718I GGTACC 1 cut(s) 1537
AspLEI GCGC 6 cut(s) 231, 265, 298, 680, 849, 1182
AspS9I GGNCC 5 cut(s) 582, 625, 1108, 1268, 1490
AsuC2I CCSGG 3 cut(s) 21, 533, 1112
AsuHPI GGTGA 7 cut(s) 628, 667, 790, 946, 1213, 1374, 1437
AsuII TTCGAA 1 cut(s) 255
AvaI CYCGRG 3 cut(s) 134, 221, 599
AvaII GGWCC 4 cut(s) 625, 1108, 1268, 1490
BaeGI GKGCMC 2 cut(s) 759, 978
BalI TGGCCA 1 cut(s) 1362
BanI GGYRCC 1 cut(s) 1537
BanII GRGCYC 3 cut(s) 114, 222, 319
BauI CACGAG 1 cut(s) 194
BbsI GAAGAC 3 cut(s) 749, 1173, 1308
Bbv12I GWGCWC 5 cut(s) 114, 195, 222, 759, 978
BbvI GCAGC 8 cut(s) 77, 238, 333, 781, 946, 1018, 1060, 1466
BccI CCATC 4 cut(s) 173, 388, 616, 689
BceAI ACGGC 2 cut(s) 367, 441
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 4 cut(s) 188, 700, 844, 1488
BciVI GTATCC 1 cut(s) 1377
BclI TGATCA 1 cut(s) 918
BcnI CCSGG 3 cut(s) 21, 533, 1112
BcoDI GTCTC 4 cut(s) 191, 241, 689, 1353
BfaI CTAG 3 cut(s) 509, 1082, 1193
BfmI CTRYAG 1 cut(s) 767
BfoI RGCGCY 2 cut(s) 299, 1183
BfuI GTATCC 1 cut(s) 1377
BlpI GCTNAGC 1 cut(s) 838
Bme1390I CCNGG 7 cut(s) 21, 188, 533, 700, 844, 1112, 1488
Bme18I GGWCC 4 cut(s) 625, 1108, 1268, 1490
BmeT110I CYCGRG 3 cut(s) 134, 221, 599
BmgT120I GGNCC 5 cut(s) 582, 625, 1108, 1268, 1490
BmiI GGNNCC 3 cut(s) 627, 1270, 1539
BmrFI CCNGG 7 cut(s) 21, 188, 533, 700, 844, 1112, 1488
BmsI GCATC 4 cut(s) 453, 846, 931, 1009
BpiI GAAGAC 3 cut(s) 749, 1173, 1308
BpmI CTGGAG 3 cut(s) 209, 423, 1146
Bpu1102I GCTNAGC 1 cut(s) 838
Bpu14I TTCGAA 1 cut(s) 255
BpuEI CTTGAG 4 cut(s) 284, 359, 1157, 1166
BpuMI CCSGG 3 cut(s) 21, 533, 1112
BsaI GGTCTC 4 cut(s) 191, 241, 689, 1353
BsaJI CCNNGG 6 cut(s) 159, 186, 201, 1284, 1357, 1486
BsaWI WCCGGW 2 cut(s) 613, 1447
Bsc4I CCNNNNNNNGG 8 cut(s) 20, 106, 187, 635, 699, 1358, 1475, 1514
Bse118I RCCGGY 1 cut(s) 1552
Bse1I ACTGG 2 cut(s) 1223, 1363
Bse3DI GCAATG 1 cut(s) 1534
BseBI CCWGG 4 cut(s) 188, 700, 844, 1488
BseDI CCNNGG 6 cut(s) 159, 186, 201, 1284, 1357, 1486
BseGI GGATG 2 cut(s) 811, 1024
BseLI CCNNNNNNNGG 8 cut(s) 20, 106, 187, 635, 699, 1358, 1475, 1514
BseMI GCAATG 1 cut(s) 1534
BseMII CTCAG 2 cut(s) 443, 852
BseNI ACTGG 2 cut(s) 1223, 1363
BseRI GAGGAG 6 cut(s) 89, 568, 731, 815, 1455, 1458
BseSI GKGCMC 2 cut(s) 759, 978
BseXI GCAGC 8 cut(s) 77, 238, 333, 781, 946, 1018, 1060, 1466
BseYI CCCAGC 3 cut(s) 579, 1064, 1373
BsgI GTGCAG 1 cut(s) 76
Bsh1236I CGCG 4 cut(s) 231, 263, 661, 678
BshFI GGCC 9 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1293, 1362
BshNI GGYRCC 1 cut(s) 1537
BsiHKAI GWGCWC 5 cut(s) 114, 195, 222, 759, 978
BsiHKCI CYCGRG 3 cut(s) 134, 221, 599
BslFI GGGAC 4 cut(s) 115, 260, 942, 1254
BslI CCNNNNNNNGG 8 cut(s) 20, 106, 187, 635, 699, 1358, 1475, 1514
BsmAI GTCTC 4 cut(s) 191, 241, 689, 1353
BsmFI GGGAC 4 cut(s) 115, 260, 942, 1254
BsmI GAATGC 1 cut(s) 1309
BsnI GGCC 9 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1293, 1362
Bso31I GGTCTC 4 cut(s) 191, 241, 689, 1353
BsoBI CYCGRG 3 cut(s) 134, 221, 599
Bsp119I TTCGAA 1 cut(s) 255
Bsp1286I GDGCHC 6 cut(s) 114, 195, 222, 319, 759, 978
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1478
Bsp1720I GCTNAGC 1 cut(s) 838
Bsp19I CCATGG 1 cut(s) 1357
BspANI GGCC 9 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1293, 1362
BspCNI CTCAG 2 cut(s) 444, 851
BspFNI CGCG 4 cut(s) 231, 263, 661, 678
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 627, 1270, 1539
BspMAI CTGCAG 1 cut(s) 771
BspPI GGATC 5 cut(s) 4, 11, 485, 1046, 1486
BspQI GCTCTTC 1 cut(s) 275
BspT104I TTCGAA 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 1537
BspTNI GGTCTC 4 cut(s) 191, 241, 689, 1353
BsrDI GCAATG 1 cut(s) 1534
BsrFI RCCGGY 1 cut(s) 1552
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 2 cut(s) 1223, 1363
BssAI RCCGGY 1 cut(s) 1552
BssECI CCNNGG 6 cut(s) 159, 186, 201, 1284, 1357, 1486
BssMI GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1478
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 3 cut(s) 201, 1284, 1357
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 4 cut(s) 188, 700, 844, 1488
Bst4CI ACNGT 1 cut(s) 1386
Bst6I CTCTTC 2 cut(s) 275, 305
BstAUI TGTACA 1 cut(s) 816
BstBI TTCGAA 1 cut(s) 255
BstC8I GCNNGC 8 cut(s) 319, 586, 709, 851, 867, 1071, 1291, 1554
BstDEI CTNAG 3 cut(s) 452, 838, 1023
BstDSI CCRYGG 1 cut(s) 1357
BstENI CCTNNNNNAGG 1 cut(s) 104
BstF5I GGATG 2 cut(s) 811, 1024
BstFNI CGCG 4 cut(s) 231, 263, 661, 678
BstH2I RGCGCY 2 cut(s) 299, 1183
BstHHI GCGC 6 cut(s) 231, 265, 298, 680, 849, 1182
BstKTI GATC 8 cut(s) 12, 19, 243, 493, 636, 921, 1054, 1481
BstMAI GTCTC 4 cut(s) 191, 241, 689, 1353
BstMBI GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1478
BstMWI GCNNNNNNNGC 8 cut(s) 217, 226, 585, 1028, 1070, 1082, 1112, 1517
BstNI CCWGG 4 cut(s) 188, 700, 844, 1488
BstSCI CCNGG 7 cut(s) 19, 186, 531, 698, 842, 1110, 1486
BstSFI CTRYAG 1 cut(s) 767
BstSLI GKGCMC 2 cut(s) 759, 978
BstUI CGCG 4 cut(s) 231, 263, 661, 678
BstV1I GCAGC 8 cut(s) 77, 238, 333, 781, 946, 1018, 1060, 1466
BstV2I GAAGAC 3 cut(s) 749, 1173, 1308
BstX2I RGATCY 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 1051
BsuI GTATCC 1 cut(s) 1377
BsuRI GGCC 9 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1293, 1362
BtgI CCRYGG 1 cut(s) 1357
BtsCI GGATG 2 cut(s) 811, 1024
BtsIMutI CAGTG 1 cut(s) 605
Cac8I GCNNGC 8 cut(s) 319, 586, 709, 851, 867, 1071, 1291, 1554
CaiI CAGNNNCTG 1 cut(s) 187
CciI TCATGA 1 cut(s) 81
CfoI GCGC 6 cut(s) 231, 265, 298, 680, 849, 1182
Cfr10I RCCGGY 1 cut(s) 1552
Cfr13I GGNCC 5 cut(s) 582, 625, 1108, 1268, 1490
CpoI CGGWCCG 1 cut(s) 1108
CseI GACGC 2 cut(s) 667, 1098
Csp6I GTAC 6 cut(s) 334, 817, 1232, 1250, 1387, 1538
CspI CGGWCCG 1 cut(s) 1108
CviAII CATG 5 cut(s) 82, 1273, 1358, 1405, 1472
CviQI GTAC 6 cut(s) 334, 817, 1232, 1250, 1387, 1538
DdeI CTNAG 3 cut(s) 452, 838, 1023
DpnI GATC 8 cut(s) 11, 18, 242, 492, 635, 920, 1053, 1480
DpnII GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1478
EaeI YGGCCR 3 cut(s) 1281, 1291, 1360
Eam1104I CTCTTC 2 cut(s) 275, 305
EarI CTCTTC 2 cut(s) 275, 305
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 2 cut(s) 112, 220
Eco130I CCWWGG 3 cut(s) 201, 1284, 1357
Eco147I AGGCCT 1 cut(s) 206
Eco24I GRGCYC 3 cut(s) 114, 222, 319
Eco31I GGTCTC 4 cut(s) 191, 241, 689, 1353
Eco32I GATATC 2 cut(s) 519, 690
Eco47I GGWCC 4 cut(s) 625, 1108, 1268, 1490
Eco53kI GAGCTC 2 cut(s) 112, 220
Eco57I CTGAAG 2 cut(s) 170, 1034
Eco88I CYCGRG 3 cut(s) 134, 221, 599
EcoICRI GAGCTC 2 cut(s) 112, 220
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoRII CCWGG 4 cut(s) 186, 698, 842, 1486
EcoRV GATATC 2 cut(s) 519, 690
EcoT14I CCWWGG 3 cut(s) 201, 1284, 1357
EcoT22I ATGCAT 2 cut(s) 1309, 1441
EcoT38I GRGCYC 3 cut(s) 114, 222, 319
ErhI CCWWGG 3 cut(s) 201, 1284, 1357
FaeI CATG 5 cut(s) 85, 1276, 1361, 1408, 1475
FalI AAGNNNNNCTT 4 cut(s) 249, 281, 695, 727
FaqI GGGAC 4 cut(s) 115, 260, 942, 1254
FatI CATG 5 cut(s) 81, 1272, 1357, 1404, 1471
FauI CCCGC 1 cut(s) 326
FauNDI CATATG 2 cut(s) 1309, 1513
FbaI TGATCA 1 cut(s) 918
FblI GTMKAC 1 cut(s) 38
FokI GGATG 2 cut(s) 818, 1031
FriOI GRGCYC 3 cut(s) 114, 222, 319
FspBI CTAG 3 cut(s) 509, 1082, 1193
GlaI GCGC 6 cut(s) 230, 264, 297, 679, 848, 1181
GsaI CCCAGC 3 cut(s) 583, 1068, 1377
GsuI CTGGAG 3 cut(s) 209, 423, 1146
HaeII RGCGCY 2 cut(s) 299, 1183
HaeIII GGCC 9 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1293, 1362
HgaI GACGC 2 cut(s) 667, 1098
HhaI GCGC 6 cut(s) 231, 265, 298, 680, 849, 1182
Hin1II CATG 5 cut(s) 85, 1276, 1361, 1408, 1475
Hin6I GCGC 6 cut(s) 229, 263, 296, 678, 847, 1180
HinP1I GCGC 6 cut(s) 229, 263, 296, 678, 847, 1180
HincII GTYRAC 2 cut(s) 30, 39
HindII GTYRAC 2 cut(s) 30, 39
HindIII AAGCTT 2 cut(s) 824, 1005
HinfI GANTC 7 cut(s) 35, 40, 370, 398, 602, 610, 745
HphI GGTGA 7 cut(s) 628, 667, 790, 946, 1213, 1374, 1437
Hpy166II GTNNAC 6 cut(s) 30, 39, 346, 538, 757, 976
Hpy188I TCNGA 7 cut(s) 118, 160, 453, 523, 694, 1014, 1497
Hpy8I GTNNAC 6 cut(s) 30, 39, 346, 538, 757, 976
Hpy99I CGWCG 5 cut(s) 59, 218, 329, 332, 353
HpyAV CCTTC 7 cut(s) 100, 430, 697, 790, 1009, 1192, 1362
HpyCH4III ACNGT 1 cut(s) 1386
HpyCH4IV ACGT 2 cut(s) 738, 761
HpyCH4V TGCA 9 cut(s) 93, 757, 769, 949, 976, 1128, 1307, 1439, 1511
HpyF10VI GCNNNNNNNGC 8 cut(s) 217, 226, 585, 1028, 1070, 1082, 1112, 1517
HpyF3I CTNAG 3 cut(s) 452, 838, 1023
HpySE526I ACGT 2 cut(s) 738, 761
Hsp92II CATG 5 cut(s) 85, 1276, 1361, 1408, 1475
HspAI GCGC 6 cut(s) 229, 263, 296, 678, 847, 1180
KpnI GGTACC 1 cut(s) 1541
KroI GCCGGC 1 cut(s) 1552
KroNI GCCGGC 1 cut(s) 1554
Ksp22I TGATCA 1 cut(s) 918
Kzo9I GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1478
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 4 cut(s) 109, 190, 555, 663
Lsp1109I GCAGC 8 cut(s) 77, 238, 333, 781, 946, 1018, 1060, 1466
LweI GCATC 4 cut(s) 453, 846, 931, 1009
MaeI CTAG 3 cut(s) 509, 1082, 1193
MaeII ACGT 2 cut(s) 738, 761
MaeIII GTNAC 4 cut(s) 421, 603, 655, 778
MalI GATC 8 cut(s) 11, 18, 242, 492, 635, 920, 1053, 1480
MboI GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1478
MflI RGATCY 1 cut(s) 1051
MhlI GDGCHC 6 cut(s) 114, 195, 222, 319, 759, 978
MlsI TGGCCA 1 cut(s) 1362
MluCI AATT 3 cut(s) 440, 914, 1154
MluNI TGGCCA 1 cut(s) 1362
MlyI GAGTC 4 cut(s) 34, 44, 611, 739
MmeI TCCRAC 2 cut(s) 141, 946
Mox20I TGGCCA 1 cut(s) 1362
Mph1103I ATGCAT 2 cut(s) 1309, 1441
MroNI GCCGGC 1 cut(s) 1552
MscI TGGCCA 1 cut(s) 1362
Msp20I TGGCCA 1 cut(s) 1362
MspA1I CMGCKG 3 cut(s) 67, 90, 1106
MspR9I CCNGG 7 cut(s) 21, 188, 533, 700, 844, 1112, 1488
Mva1269I GAATGC 1 cut(s) 1309
MvaI CCWGG 4 cut(s) 188, 700, 844, 1488
MvnI CGCG 4 cut(s) 231, 263, 661, 678
MwoI GCNNNNNNNGC 8 cut(s) 217, 226, 585, 1028, 1070, 1082, 1112, 1517
NaeI GCCGGC 1 cut(s) 1554
NciI CCSGG 3 cut(s) 21, 533, 1112
NcoI CCATGG 1 cut(s) 1357
NdeI CATATG 2 cut(s) 1309, 1513
NdeII GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1478
NgoMIV GCCGGC 1 cut(s) 1552
NlaIII CATG 5 cut(s) 85, 1276, 1361, 1408, 1475
NlaIV GGNNCC 3 cut(s) 627, 1270, 1539
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 4 cut(s) 421, 603, 655, 778
NsiI ATGCAT 2 cut(s) 1309, 1441
NspV TTCGAA 1 cut(s) 255
PaeR7I CTCGAG 3 cut(s) 134, 221, 599
PagI TCATGA 1 cut(s) 81
PceI AGGCCT 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 275
PcsI WCGNNNNNNNCGW 4 cut(s) 44, 219, 228, 690
PctI GAATGC 1 cut(s) 1309
PdiI GCCGGC 1 cut(s) 1554
PfeI GAWTC 3 cut(s) 370, 398, 610
PflFI GACNNNGTC 1 cut(s) 34
PflMI CCANNNNNTGG 1 cut(s) 187
PleI GAGTC 4 cut(s) 34, 43, 610, 739
PpsI GAGTC 4 cut(s) 34, 43, 610, 739
PsiI TTATAA 1 cut(s) 1140
Psp124BI GAGCTC 2 cut(s) 114, 222
Psp6I CCWGG 4 cut(s) 186, 698, 842, 1486
PspFI CCCAGC 3 cut(s) 579, 1064, 1373
PspGI CCWGG 4 cut(s) 186, 698, 842, 1486
PspN4I GGNNCC 3 cut(s) 627, 1270, 1539
PspPI GGNCC 5 cut(s) 582, 625, 1108, 1268, 1490
PspXI VCTCGAGB 3 cut(s) 134, 221, 599
PstI CTGCAG 1 cut(s) 771
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 1051
PsyI GACNNNGTC 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 90
RsaI GTAC 6 cut(s) 335, 818, 1233, 1251, 1388, 1539
RsaNI GTAC 6 cut(s) 334, 817, 1232, 1250, 1387, 1538
Rsr2I CGGWCCG 1 cut(s) 1108
RsrII CGGWCCG 1 cut(s) 1108
SacI GAGCTC 2 cut(s) 114, 222
SalI GTCGAC 1 cut(s) 37
SapI GCTCTTC 1 cut(s) 275
Sau3AI GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1478
Sau96I GGNCC 5 cut(s) 582, 625, 1108, 1268, 1490
SchI GAGTC 4 cut(s) 34, 44, 611, 739
ScrFI CCNGG 7 cut(s) 21, 188, 533, 700, 844, 1112, 1488
SduI GDGCHC 6 cut(s) 114, 195, 222, 319, 759, 978
SfaNI GCATC 4 cut(s) 453, 846, 931, 1009
SfcI CTRYAG 1 cut(s) 767
Sfr274I CTCGAG 3 cut(s) 134, 221, 599
SfuI TTCGAA 1 cut(s) 255
SinI GGWCC 4 cut(s) 625, 1108, 1268, 1490
SlaI CTCGAG 3 cut(s) 134, 221, 599
SmlI CTYRAG 7 cut(s) 134, 221, 299, 374, 599, 1145, 1172
SmoI CTYRAG 7 cut(s) 134, 221, 299, 374, 599, 1145, 1172
Sse9I AATT 3 cut(s) 440, 914, 1154
SseBI AGGCCT 1 cut(s) 206
SspMI CTAG 3 cut(s) 509, 1082, 1193
SstI GAGCTC 2 cut(s) 114, 222
StuI AGGCCT 1 cut(s) 206
StyD4I CCNGG 7 cut(s) 19, 186, 531, 698, 842, 1110, 1486
StyI CCWWGG 3 cut(s) 201, 1284, 1357
TaaI ACNGT 1 cut(s) 1386
TaiI ACGT 2 cut(s) 741, 764
TaqII GACCGA 1 cut(s) 47
TasI AATT 3 cut(s) 440, 914, 1154
TatI WGTACW 1 cut(s) 816
TauI GCSGC 5 cut(s) 70, 296, 729, 1037, 1296
TfiI GAWTC 3 cut(s) 370, 398, 610
TscAI CASTG 1 cut(s) 612
TseFI GTSAC 4 cut(s) 421, 603, 655, 778
TseI GCWGC 8 cut(s) 90, 226, 321, 769, 934, 1031, 1073, 1454
Tsp45I GTSAC 4 cut(s) 421, 603, 655, 778
TspDTI ATGAA 2 cut(s) 383, 1514
TspRI CASTG 1 cut(s) 612
Tth111I GACNNNGTC 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 187
VneI GTGCAC 2 cut(s) 755, 974
VpaK11BI GGWCC 4 cut(s) 625, 1108, 1268, 1490
XagI CCTNNNNNAGG 1 cut(s) 104
XapI RAATTY 1 cut(s) 1154
XcmI CCANNNNNNNNNTGG 1 cut(s) 1370
XhoI CTCGAG 3 cut(s) 134, 221, 599
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 3 cut(s) 509, 1082, 1193
Zsp2I ATGCAT 2 cut(s) 1309, 1441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.