pycom16g16370

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010357
Physical Location & Seq
Forward (+)
12311323 .. 12313013
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16370.1

Sequence Viewer

Length: 942 bp
ATGGGGTCGATCACCGATCCCGGCGAGTTGACTGAGTTGACTCCGCCGAGCTTCGACGAGTTCCAGCGGCAGACCTCTCTCATGACCAGCTGCACTCTCCTCTGGAAGGAGCTCTCCGACCACTTCTCGTCCCTCGAGCAGAACCTCCTGAAGAAATCCGAGGCCATCAAGCTCAAAATCCAGACTCTGGAGCAAGAGACCAAGGTGTCGCTCGACGAGCTCGAGCAGCGCGAGGTCACGATCAGGGACAGCTTCGAGATCGCGCTCGGGAAGGTCGAGAAGACCAAGGAGTCGGCGCTCAAGGTGTTGAAGAGAGGCCGCAGCGACGATGAGTACGGGGAGGTGGACGACGGCGAGGGATTGCTGATGAATCTCAAGTCGTTGTGCTTGAAGATGGATTCTGGAGGGCTCTGGAGGTTCGTGACAGCGAGGAAGAAGGAATTGGAGGGTCTGAGGTCGCAGATGCCTGTGGCTTTGGGCGATTGTGTCGATCCGGCGAGGTTCGTGCTGGAGGCGATATCGGAGGTGTTTCCTGTGGACAAGAGACTGGAGAGGAGCGATAGGGGAAACGATTTGGGCTGGGCCTGCGTTCTGTTGCTGGAGTCGCTGATTCCGGTGGTGGTGGACCCGAAGATCGGGAGAAAAAGGCTTCTGGTGACGCGGAGCGTGAAGCAACGCGCCACGGAGATGGCCGAGACTTGGAAAGCCAGCTTGGAGGAGAGAGGCGGCATAGAGAACGTGAAGACTCCTGATGTGCACACGTTCCTGCAGCATTTGGTGACTTTCGGGATTGTGAAGGAGGAGGATGTTGATTTGTACAGAAAGCTTGTGGTTAGCTCAGCCTGGCGCAAGCAGATGCCAAAGCTCGCCGTTTCGCTTGGCCTCGCCAAGCAAATGCCCGATATGATTGAAGAATTGATCGAGCAGGGGACAACAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

314

Amino Acids

35.31

Weight (kDa)

5.27

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 123 - 311 2.8e-63 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 205, 289
AccB7I CCANNNNNTGG 1 cut(s) 187
AccII CGCG 4 cut(s) 231, 263, 661, 678
AciI CCGC 5 cut(s) 44, 67, 319, 661, 726
AclWI GGATC 2 cut(s) 11, 485
AcoI YGGCCR 1 cut(s) 690
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 2 cut(s) 335, 818
AfiI CCNNNNNNNGG 4 cut(s) 106, 187, 635, 699
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 3 cut(s) 310, 391, 911
AjnI CCWGG 1 cut(s) 842
AjuI GAANNNNNNNTTGG 4 cut(s) 425, 457, 695, 727
Alw21I GWGCWC 3 cut(s) 114, 222, 759
Alw26I GTCTC 3 cut(s) 191, 538, 689
Alw44I GTGCAC 1 cut(s) 755
AlwI GGATC 2 cut(s) 11, 485
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 3 cut(s) 134, 221, 266
AoxI GGCC 5 cut(s) 162, 316, 582, 690, 880
ApaLI GTGCAC 1 cut(s) 755
ApeKI GCWGC 4 cut(s) 90, 226, 321, 769
AspLEI GCGC 5 cut(s) 231, 265, 298, 680, 849
AspS9I GGNCC 2 cut(s) 582, 625
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 3 cut(s) 4, 667, 790
AvaI CYCGRG 3 cut(s) 134, 221, 266
AvaII GGWCC 1 cut(s) 625
BaeGI GKGCMC 1 cut(s) 759
BanII GRGCYC 3 cut(s) 114, 222, 411
BbsI GAAGAC 2 cut(s) 287, 749
Bbv12I GWGCWC 3 cut(s) 114, 222, 759
BbvI GCAGC 4 cut(s) 77, 238, 333, 781
BccI CCATC 3 cut(s) 173, 388, 682
BceAI ACGGC 2 cut(s) 367, 854
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 1 cut(s) 844
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 3 cut(s) 191, 538, 689
BfmI CTRYAG 1 cut(s) 767
BfoI RGCGCY 1 cut(s) 299
BisI GCNGC 7 cut(s) 68, 91, 227, 319, 322, 727, 770
BlpI GCTNAGC 1 cut(s) 838
BlsI GCNGC 7 cut(s) 69, 92, 228, 320, 323, 728, 771
Bme1390I CCNGG 2 cut(s) 21, 844
Bme18I GGWCC 1 cut(s) 625
BmeT110I CYCGRG 3 cut(s) 134, 221, 266
BmgT120I GGNCC 2 cut(s) 582, 625
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 2 cut(s) 21, 844
BmsI GCATC 2 cut(s) 453, 846
BpiI GAAGAC 2 cut(s) 287, 749
BpmI CTGGAG 6 cut(s) 209, 423, 433, 530, 569, 620
Bpu1102I GCTNAGC 1 cut(s) 838
BpuEI CTTGAG 2 cut(s) 284, 359
BpuMI CCSGG 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 4 cut(s) 159, 201, 285, 681
BsaWI WCCGGW 1 cut(s) 613
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 187, 635, 699
Bse1I ACTGG 1 cut(s) 552
BseBI CCWGG 1 cut(s) 844
BseDI CCNNGG 4 cut(s) 159, 201, 285, 681
BseGI GGATG 1 cut(s) 811
BseLI CCNNNNNNNGG 4 cut(s) 106, 187, 635, 699
BseMII CTCAG 3 cut(s) 24, 443, 852
BseNI ACTGG 1 cut(s) 552
BseRI GAGGAG 4 cut(s) 89, 568, 731, 815
BseSI GKGCMC 1 cut(s) 759
BseXI GCAGC 4 cut(s) 77, 238, 333, 781
BseYI CCCAGC 1 cut(s) 579
BsgI GTGCAG 1 cut(s) 76
Bsh1236I CGCG 4 cut(s) 231, 263, 661, 678
BshFI GGCC 5 cut(s) 164, 318, 584, 692, 882
BsiHKAI GWGCWC 3 cut(s) 114, 222, 759
BsiHKCI CYCGRG 3 cut(s) 134, 221, 266
BsiSI CCGG 3 cut(s) 21, 494, 614
BslFI GGGAC 2 cut(s) 115, 260
BslI CCNNNNNNNGG 4 cut(s) 106, 187, 635, 699
BsmAI GTCTC 3 cut(s) 191, 538, 689
BsmFI GGGAC 2 cut(s) 115, 260
BsnI GGCC 5 cut(s) 164, 318, 584, 692, 882
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 3 cut(s) 134, 221, 266
Bsp1286I GDGCHC 4 cut(s) 114, 222, 411, 759
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 7 cut(s) 9, 16, 240, 258, 490, 633, 918
Bsp1720I GCTNAGC 1 cut(s) 838
BspACI CCGC 5 cut(s) 44, 67, 319, 661, 726
BspANI GGCC 5 cut(s) 164, 318, 584, 692, 882
BspCNI CTCAG 3 cut(s) 25, 444, 851
BspFNI CGCG 4 cut(s) 231, 263, 661, 678
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 627
BspMAI CTGCAG 1 cut(s) 771
BspPI GGATC 2 cut(s) 11, 485
BspTNI GGTCTC 1 cut(s) 191
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 1 cut(s) 552
BssECI CCNNGG 4 cut(s) 159, 201, 285, 681
BssMI GATC 7 cut(s) 9, 16, 240, 258, 490, 633, 918
BssT1I CCWWGG 2 cut(s) 201, 285
Bst2UI CCWGG 1 cut(s) 844
Bst6I CTCTTC 1 cut(s) 305
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 4 cut(s) 586, 709, 851, 867
BstDEI CTNAG 3 cut(s) 33, 452, 838
BstDSI CCRYGG 1 cut(s) 681
BstENI CCTNNNNNAGG 1 cut(s) 104
BstF5I GGATG 1 cut(s) 811
BstFNI CGCG 4 cut(s) 231, 263, 661, 678
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 5 cut(s) 231, 265, 298, 680, 849
BstKTI GATC 7 cut(s) 12, 19, 243, 261, 493, 636, 921
BstMAI GTCTC 3 cut(s) 191, 538, 689
BstMBI GATC 7 cut(s) 9, 16, 240, 258, 490, 633, 918
BstMWI GCNNNNNNNGC 4 cut(s) 217, 226, 585, 604
BstNI CCWGG 1 cut(s) 844
BstSCI CCNGG 2 cut(s) 19, 842
BstSFI CTRYAG 1 cut(s) 767
BstSLI GKGCMC 1 cut(s) 759
BstUI CGCG 4 cut(s) 231, 263, 661, 678
BstV1I GCAGC 4 cut(s) 77, 238, 333, 781
BstV2I GAAGAC 2 cut(s) 287, 749
BstXI CCANNNNNNTGG 1 cut(s) 688
BsuRI GGCC 5 cut(s) 164, 318, 584, 692, 882
BtgI CCRYGG 1 cut(s) 681
BtsCI GGATG 1 cut(s) 811
Cac8I GCNNGC 4 cut(s) 586, 709, 851, 867
CaiI CAGNNNCTG 1 cut(s) 187
CciI TCATGA 1 cut(s) 81
CfoI GCGC 5 cut(s) 231, 265, 298, 680, 849
Cfr13I GGNCC 2 cut(s) 582, 625
CseI GACGC 1 cut(s) 667
Csp6I GTAC 2 cut(s) 334, 817
CviAII CATG 1 cut(s) 82
CviQI GTAC 2 cut(s) 334, 817
DdeI CTNAG 3 cut(s) 33, 452, 838
DpnI GATC 7 cut(s) 11, 18, 242, 260, 492, 635, 920
DpnII GATC 7 cut(s) 9, 16, 240, 258, 490, 633, 918
DrdI GACNNNNNNGTC 2 cut(s) 205, 289
DseDI GACNNNNNNGTC 2 cut(s) 205, 289
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 2 cut(s) 112, 220
Eco130I CCWWGG 2 cut(s) 201, 285
Eco24I GRGCYC 3 cut(s) 114, 222, 411
Eco31I GGTCTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 519
Eco47I GGWCC 1 cut(s) 625
Eco53kI GAGCTC 2 cut(s) 112, 220
Eco57I CTGAAG 1 cut(s) 170
Eco88I CYCGRG 3 cut(s) 134, 221, 266
EcoICRI GAGCTC 2 cut(s) 112, 220
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 842
EcoRV GATATC 1 cut(s) 519
EcoT14I CCWWGG 2 cut(s) 201, 285
EcoT38I GRGCYC 3 cut(s) 114, 222, 411
ErhI CCWWGG 2 cut(s) 201, 285
FaeI CATG 1 cut(s) 85
FaiI YATR 3 cut(s) 83, 731, 905
FaqI GGGAC 2 cut(s) 115, 260
FatI CATG 1 cut(s) 81
Fnu4HI GCNGC 7 cut(s) 68, 91, 227, 319, 322, 727, 770
FokI GGATG 1 cut(s) 818
FriOI GRGCYC 3 cut(s) 114, 222, 411
Fsp4HI GCNGC 7 cut(s) 68, 91, 227, 319, 322, 727, 770
GlaI GCGC 5 cut(s) 230, 264, 297, 679, 848
GluI GCNGC 7 cut(s) 68, 91, 227, 319, 322, 727, 770
GsaI CCCAGC 1 cut(s) 583
GsuI CTGGAG 6 cut(s) 209, 423, 433, 530, 569, 620
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 5 cut(s) 164, 318, 584, 692, 882
HapII CCGG 3 cut(s) 21, 494, 614
HgaI GACGC 1 cut(s) 667
HhaI GCGC 5 cut(s) 231, 265, 298, 680, 849
Hin1II CATG 1 cut(s) 85
Hin6I GCGC 5 cut(s) 229, 263, 296, 678, 847
HinP1I GCGC 5 cut(s) 229, 263, 296, 678, 847
HincII GTYRAC 2 cut(s) 30, 39
HindII GTYRAC 2 cut(s) 30, 39
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 8 cut(s) 40, 184, 290, 370, 398, 602, 610, 745
HpaII CCGG 3 cut(s) 21, 494, 614
HphI GGTGA 3 cut(s) 4, 667, 790
Hpy166II GTNNAC 6 cut(s) 30, 39, 346, 538, 625, 757
Hpy188I TCNGA 4 cut(s) 118, 160, 453, 523
Hpy8I GTNNAC 6 cut(s) 30, 39, 346, 538, 625, 757
Hpy99I CGWCG 4 cut(s) 59, 218, 329, 353
HpyAV CCTTC 4 cut(s) 100, 265, 430, 790
HpyCH4IV ACGT 2 cut(s) 738, 761
HpyCH4V TGCA 3 cut(s) 93, 757, 769
HpyF10VI GCNNNNNNNGC 4 cut(s) 217, 226, 585, 604
HpyF3I CTNAG 3 cut(s) 33, 452, 838
HpySE526I ACGT 2 cut(s) 738, 761
Hsp92II CATG 1 cut(s) 85
HspAI GCGC 5 cut(s) 229, 263, 296, 678, 847
Kzo9I GATC 7 cut(s) 9, 16, 240, 258, 490, 633, 918
LmnI GCTCC 4 cut(s) 109, 190, 555, 663
Lsp1109I GCAGC 4 cut(s) 77, 238, 333, 781
LweI GCATC 2 cut(s) 453, 846
MaeII ACGT 2 cut(s) 738, 761
MaeIII GTNAC 4 cut(s) 235, 421, 655, 778
MalI GATC 7 cut(s) 11, 18, 242, 260, 492, 635, 920
MboI GATC 7 cut(s) 9, 16, 240, 258, 490, 633, 918
MboII GAAGA 8 cut(s) 163, 292, 322, 403, 445, 643, 754, 923
MhlI GDGCHC 4 cut(s) 114, 222, 411, 759
MluCI AATT 2 cut(s) 440, 914
MlyI GAGTC 5 cut(s) 34, 178, 299, 611, 739
MmeI TCCRAC 1 cut(s) 141
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 2 cut(s) 67, 90
MspI CCGG 3 cut(s) 21, 494, 614
MspR9I CCNGG 2 cut(s) 21, 844
MvaI CCWGG 1 cut(s) 844
MvnI CGCG 4 cut(s) 231, 263, 661, 678
MwoI GCNNNNNNNGC 4 cut(s) 217, 226, 585, 604
NciI CCSGG 1 cut(s) 21
NdeII GATC 7 cut(s) 9, 16, 240, 258, 490, 633, 918
NlaIII CATG 1 cut(s) 85
NlaIV GGNNCC 1 cut(s) 627
NmeAIII GCCGAG 2 cut(s) 72, 718
NmuCI GTSAC 4 cut(s) 235, 421, 655, 778
PaeR7I CTCGAG 2 cut(s) 134, 221
PagI TCATGA 1 cut(s) 81
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 228, 273
PfeI GAWTC 3 cut(s) 370, 398, 610
PflMI CCANNNNNTGG 1 cut(s) 187
PkrI GCNGC 7 cut(s) 69, 92, 228, 320, 323, 728, 771
PleI GAGTC 5 cut(s) 34, 178, 298, 610, 739
PpsI GAGTC 5 cut(s) 34, 178, 298, 610, 739
Psp124BI GAGCTC 2 cut(s) 114, 222
Psp6I CCWGG 1 cut(s) 842
PspFI CCCAGC 1 cut(s) 579
PspGI CCWGG 1 cut(s) 842
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 2 cut(s) 582, 625
PspXI VCTCGAGB 2 cut(s) 134, 221
PstI CTGCAG 1 cut(s) 771
PstNI CAGNNNCTG 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 90
RsaI GTAC 2 cut(s) 335, 818
RsaNI GTAC 2 cut(s) 334, 817
RseI CAYNNNNRTG 1 cut(s) 686
SacI GAGCTC 2 cut(s) 114, 222
SatI GCNGC 7 cut(s) 68, 91, 227, 319, 322, 727, 770
Sau3AI GATC 7 cut(s) 9, 16, 240, 258, 490, 633, 918
Sau96I GGNCC 2 cut(s) 582, 625
SchI GAGTC 5 cut(s) 34, 178, 299, 611, 739
ScrFI CCNGG 2 cut(s) 21, 844
SduI GDGCHC 4 cut(s) 114, 222, 411, 759
SfaNI GCATC 2 cut(s) 453, 846
SfcI CTRYAG 1 cut(s) 767
Sfr274I CTCGAG 2 cut(s) 134, 221
SinI GGWCC 1 cut(s) 625
SlaI CTCGAG 2 cut(s) 134, 221
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 4 cut(s) 134, 221, 299, 374
SmoI CTYRAG 4 cut(s) 134, 221, 299, 374
Sse9I AATT 2 cut(s) 440, 914
SsiI CCGC 5 cut(s) 44, 67, 319, 661, 726
SstI GAGCTC 2 cut(s) 114, 222
StyD4I CCNGG 2 cut(s) 19, 842
StyI CCWWGG 2 cut(s) 201, 285
TaiI ACGT 2 cut(s) 741, 764
TaqI TCGA 9 cut(s) 8, 54, 135, 213, 222, 255, 276, 489, 921
TasI AATT 2 cut(s) 440, 914
TatI WGTACW 1 cut(s) 816
TauI GCSGC 3 cut(s) 70, 321, 729
TfiI GAWTC 3 cut(s) 370, 398, 610
TseFI GTSAC 4 cut(s) 235, 421, 655, 778
TseI GCWGC 4 cut(s) 90, 226, 321, 769
Tsp45I GTSAC 4 cut(s) 235, 421, 655, 778
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 698
Van91I CCANNNNNTGG 1 cut(s) 187
VneI GTGCAC 1 cut(s) 755
VpaK11BI GGWCC 1 cut(s) 625
XagI CCTNNNNNAGG 1 cut(s) 104
XhoI CTCGAG 2 cut(s) 134, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.