Rorug04G0139900

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010357
Physical Location & Seq
Reverse (-)
22951346 .. 22954558
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0139900.1

Sequence Viewer

Length: 450 bp
ATGGATCACATGGCAGCGATGGAAGAGCAATTGGTATCAGAGAGGGTTAAACAAAAGCTACAAGACGTGAATGCGGCTGCCCAAAAGTATCTCTCTCCGGTCCAAGACCATGTCAATTTCACCCTACAGCAAGCATACTTTAAATGTGCATACGAGTGTTTTGATAGGAGGAGAAGTGAACAAGAGATTGGCAATTGCGTTGAAAATTGTAGCGTGCCTGTTGTTAGTGCTCAACAGAGAGTTGAGAATGAGATGGCCAAGTTTCAAGAAAGGTTGAGTAGGGCACTGATGGTCTGTCAAGACAAGTTTGATGCAGCTAAGCTTCAACAGGTCAAAACTAGTGCACTCAATGATTTGGAGTCATGTGTTCAACAAACGACCGAAGATGGAATAAAGACACTTCCACTTCTTGCTGAAAGATTGAAGTCTTCCTTTTCGATCAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

149

Amino Acids

17.0

Weight (kDa)

5.45

Isoelectric Point (pI)

62.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF842 PF05811 20 - 143 2e-37 Eukaryotic protein of unknown function (DUF842)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 255
AgsI TTSAA 5 cut(s) 203, 266, 326, 371, 424
AhlI ACTAGT 1 cut(s) 338
AjiI CACGTC 1 cut(s) 67
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AluBI AGCT 3 cut(s) 58, 317, 322
AluI AGCT 3 cut(s) 58, 317, 322
Alw21I GWGCWC 2 cut(s) 232, 346
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 255
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 3 cut(s) 14, 77, 314
AspS9I GGNCC 1 cut(s) 100
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 1 cut(s) 100
BaeGI GKGCMC 2 cut(s) 286, 346
BalI TGGCCA 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 232, 346
BbvI GCAGC 3 cut(s) 26, 64, 326
BccI CCATC 4 cut(s) 13, 247, 283, 380
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 1 cut(s) 339
BfmI CTRYAG 1 cut(s) 125
BisI GCNGC 4 cut(s) 15, 75, 78, 315
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 4 cut(s) 16, 76, 79, 316
Bme18I GGWCC 1 cut(s) 100
BmgBI CACGTC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 100
BmsI GCATC 1 cut(s) 301
BpiI GAAGAC 1 cut(s) 420
Bpu1102I GCTNAGC 1 cut(s) 318
BsaWI WCCGGW 1 cut(s) 97
BsaXI ACNNNNNCTCC 2 cut(s) 160, 190
BseRI GAGGAG 1 cut(s) 184
BseSI GKGCMC 2 cut(s) 286, 346
BseXI GCAGC 3 cut(s) 26, 64, 326
Bsh1285I CGRYCG 1 cut(s) 381
BshFI GGCC 1 cut(s) 257
BsiEI CGRYCG 1 cut(s) 381
BsiHKAI GWGCWC 2 cut(s) 232, 346
BsiSI CCGG 1 cut(s) 98
BsmI GAATGC 1 cut(s) 76
BsnI GGCC 1 cut(s) 257
Bsp1286I GDGCHC 3 cut(s) 232, 286, 346
Bsp143I GATC 2 cut(s) 4, 438
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 257
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 18
BssMI GATC 2 cut(s) 4, 438
Bst6I CTCTTC 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 132, 215
BstDEI CTNAG 1 cut(s) 318
BstKTI GATC 2 cut(s) 7, 441
BstMBI GATC 2 cut(s) 4, 438
BstMCI CGRYCG 1 cut(s) 381
BstSFI CTRYAG 1 cut(s) 125
BstSLI GKGCMC 2 cut(s) 286, 346
BstV1I GCAGC 3 cut(s) 26, 64, 326
BstV2I GAAGAC 1 cut(s) 420
BsuRI GGCC 1 cut(s) 257
BtgZI GCGATG 1 cut(s) 32
BtrI CACGTC 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 284
Cac8I GCNNGC 2 cut(s) 132, 215
Cfr13I GGNCC 1 cut(s) 100
CviAII CATG 3 cut(s) 10, 110, 363
CviJI RGCY 5 cut(s) 58, 77, 257, 317, 322
CviKI_1 RGCY 5 cut(s) 58, 77, 257, 317, 322
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 6, 440
DpnII GATC 2 cut(s) 4, 438
DraI TTTAAA 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 100
FaeI CATG 3 cut(s) 13, 113, 366
FaiI YATR 5 cut(s) 11, 111, 136, 151, 364
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FatI CATG 3 cut(s) 9, 109, 362
Fnu4HI GCNGC 4 cut(s) 15, 75, 78, 315
Fsp4HI GCNGC 4 cut(s) 15, 75, 78, 315
FspBI CTAG 1 cut(s) 339
GluI GCNGC 4 cut(s) 15, 75, 78, 315
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 98
Hin1II CATG 3 cut(s) 13, 113, 366
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 98
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 179, 344
Hpy188I TCNGA 1 cut(s) 40
Hpy188III TCNNGA 2 cut(s) 266, 299
Hpy8I GTNNAC 2 cut(s) 179, 344
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 3 cut(s) 149, 314, 344
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 3 cut(s) 13, 113, 366
Kzo9I GATC 2 cut(s) 4, 438
LguI GCTCTTC 1 cut(s) 18
LpnPI CCDG 3 cut(s) 111, 231, 314
Lsp1109I GCAGC 3 cut(s) 26, 64, 326
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 339
MaeII ACGT 1 cut(s) 66
MalI GATC 2 cut(s) 6, 440
MboI GATC 2 cut(s) 4, 438
MboII GAAGA 3 cut(s) 35, 395, 420
MfeI CAATTG 2 cut(s) 29, 193
MhlI GDGCHC 3 cut(s) 232, 286, 346
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 5 cut(s) 29, 115, 193, 205, 445
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 2 cut(s) 36, 162
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MseI TTAA 3 cut(s) 48, 141, 448
MslI CAYNNNNRTG 1 cut(s) 154
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 1 cut(s) 98
MunI CAATTG 2 cut(s) 29, 193
Mva1269I GAATGC 1 cut(s) 76
NdeII GATC 2 cut(s) 4, 438
NlaIII CATG 3 cut(s) 13, 113, 366
PciSI GCTCTTC 1 cut(s) 18
PctI GAATGC 1 cut(s) 76
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 4 cut(s) 16, 76, 79, 316
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PspPI GGNCC 1 cut(s) 100
PsyI GACNNNGTC 1 cut(s) 110
RseI CAYNNNNRTG 1 cut(s) 154
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 3 cut(s) 48, 141, 448
SatI GCNGC 4 cut(s) 15, 75, 78, 315
Sau3AI GATC 2 cut(s) 4, 438
Sau96I GGNCC 1 cut(s) 100
SchI GAGTC 1 cut(s) 368
SduI GDGCHC 3 cut(s) 232, 286, 346
SetI ASST 6 cut(s) 60, 69, 275, 319, 324, 333
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 125
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 154
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 5 cut(s) 29, 115, 193, 205, 445
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 339
TaiI ACGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 437
TaqII GACCGA 1 cut(s) 395
TasI AATT 5 cut(s) 29, 115, 193, 205, 445
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 3 cut(s) 48, 141, 448
Tru9I TTAA 3 cut(s) 48, 141, 448
TscAI CASTG 1 cut(s) 291
TseI GCWGC 3 cut(s) 14, 77, 314
TspRI CASTG 1 cut(s) 291
Tth111I GACNNNGTC 1 cut(s) 110
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 100
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.