MD16G1194000.v1.1

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010357
Physical Location & Seq
Forward (+)
17213384 .. 17217390
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1194000.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGGGGTCGATCCCCGATCCCGGCGAGTTGACTGAGTTGACTCCGCCGAGCTTCGACGAGTTCCAGCGGCAGACCTCTCTCATGACCAGCTGCACTCTCCTCTGGAAGGAGCTCTCCGACCACTTCTCGTCCCTCGAGCAGAACCTCCTGAAGAAATCTGAGGCCATCAAGCTCAAAATCCAGACTCTGGAGCAAGAGACCAAGGCGTCGCTCGACGAGCTCGAGCAGCGCGAGGTCACGATCAGGGACAGCTTCGAGATCGCGCTCGGGAAGGTCGAGAAGAGCAAGGAGGCGGCGCTCAAGGTGTTGAAGAGAGGCCGCAGCGACGATGAGTACGGGGAGGTGGACGACGGCGAGGGATTGCTGATGAATCTCAAGTCGTTGTGCTTGAAGATGGATTCTGGAGGGTTCTGGAGGTTCGTGACGGCGAGGAAGAAGGAATTGGAGGGTCTGAGGTCGCAGATGCCTGTGGCTTTGGGCGATTGTGTCGATCCGGCGAAGTTCGTGCTGGAGGCGATATCGGAGGTGTTTCCTGTGGACAAGAGACTGGAGAGGAGCGATAGGGGAAACGATTTGGGCTGGGCCTGCGTTCTGTTGCTGGAGTCGCTGATTCCGGTGGTGGTGGACCCGAAGATCGGGAGAAAGAGGCTTCTGGTGACGCGGAGCGTGAAGCAACGCGCCACGGAGATGGCCGAGACTTGGAAGGCCAGCTTGGAGGAGAGAGGCGGCATAGAGAACGTGAAGACTCCTGATGTGCACACGTTCCTGCAGCATTTGGTGACTTTCGGGATTGTGAAGGAGGAGGATGTTGATTTTTATAGAAAGCTTGTGGTTAGCTCAGCCTGGCGCAAGCAGATGCCAAAGCTCGCCGTTTCGCTTGGCCTCGCCAAGCAAATGCCTGATATGATTGAAGAATTGATCAGCAGGGGACAACAGCTTGATGCGGTGCATTTCACCTATGAAGTTGGACTTGTGCACAAGTTTCCTCCTGTTCCTCTACTGAAAGCTTTTCTGAAAGATGCTAAGAAGGCTGCAGCCTCTATTTTGGAAGATCCCAATAATGCTGGGCGAGCTGCGAACCTAGCTGGACGCAAAGAGCTGTCAGCGCTTCGGGCTGTCGTGAAGTGCATTGAAGATTATAACTTAGAGACAGAATTTCCTCCAGAAAACCTCAAGAAGCGCCTTGAACAGCTAGAGAAGGTGAAACCAGAGAAGAAAAGGCCAGTTGTAGTCCCAGCCAACAAGCGAACACGAGCTAACAACGGTGGTCCCATGCCTCCAGCCAAGGCCGGACGCTTGACGAATGCATATGTGTCTTCTTTCCCTACAACTCCACCTACATTTGTCATGTCTCCATCCCATGGCCAGTATCCTGCTGGGTTCTCACCGTACCATTCCCCACCCACCATGTATGGCAGCAGAAGCCCACCAACCCCTTATGCATACTCGCCCGAAGCAGCCCCTCCTCCTGCCGGATCATACCCTGGAGCTCCGATGAACTATCCCGCCTATGCAGCTTATGGCAACGGTATGGTACCAGCTTATCAGCAGGCTTACTACCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

522

Amino Acids

57.97

Weight (kDa)

8.51

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 123 - 408 8.8e-106 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
AasI GACNNNNNNGTC 1 cut(s) 205
Acc65I GGTACC 1 cut(s) 1534
AccB1I GGYRCC 1 cut(s) 1534
AccB7I CCANNNNNTGG 2 cut(s) 187, 1361
AccII CGCG 4 cut(s) 231, 263, 661, 678
AciI CCGC 8 cut(s) 44, 67, 293, 319, 661, 726, 944, 1506
AclWI GGATC 5 cut(s) 4, 11, 485, 1046, 1483
AcoI YGGCCR 2 cut(s) 690, 1363
AcsI RAATTY 1 cut(s) 1154
AcuI CTGAAG 1 cut(s) 170
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 3 cut(s) 335, 1391, 1536
AfeI AGCGCT 1 cut(s) 1107
AfiI CCNNNNNNNGG 7 cut(s) 20, 106, 187, 635, 699, 1361, 1472
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 5 cut(s) 310, 391, 911, 1133, 1187
AjnI CCWGG 2 cut(s) 842, 1483
AjuI GAANNNNNNNTTGG 4 cut(s) 425, 457, 695, 727
Alw21I GWGCWC 5 cut(s) 114, 222, 759, 978, 1492
Alw26I GTCTC 5 cut(s) 191, 538, 689, 1142, 1356
Alw44I GTGCAC 2 cut(s) 755, 974
AlwI GGATC 5 cut(s) 4, 11, 485, 1046, 1483
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 3 cut(s) 134, 221, 266
Aor51HI AGCGCT 1 cut(s) 1107
AoxI GGCC 9 cut(s) 162, 316, 582, 690, 705, 880, 1220, 1287, 1363
ApaLI GTGCAC 2 cut(s) 755, 974
ApoI RAATTY 1 cut(s) 1154
Asp718I GGTACC 1 cut(s) 1534
AspLEI GCGC 7 cut(s) 231, 265, 298, 680, 849, 1108, 1182
AspS9I GGNCC 3 cut(s) 582, 625, 1268
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 5 cut(s) 667, 790, 946, 1213, 1377
AvaI CYCGRG 3 cut(s) 134, 221, 266
AvaII GGWCC 2 cut(s) 625, 1268
BaeGI GKGCMC 2 cut(s) 759, 978
BalI TGGCCA 1 cut(s) 1365
BanI GGYRCC 1 cut(s) 1534
BanII GRGCYC 3 cut(s) 114, 222, 1492
BauI CACGAG 1 cut(s) 1251
BbsI GAAGAC 2 cut(s) 749, 1308
Bbv12I GWGCWC 5 cut(s) 114, 222, 759, 978, 1492
BccI CCATC 4 cut(s) 173, 388, 682, 1363
BceAI ACGGC 3 cut(s) 367, 441, 854
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 2 cut(s) 844, 1485
BciVI GTATCC 1 cut(s) 1380
BclI TGATCA 1 cut(s) 918
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 5 cut(s) 191, 538, 689, 1142, 1356
BfaI CTAG 2 cut(s) 1082, 1193
BfmI CTRYAG 2 cut(s) 767, 1032
BfoI RGCGCY 3 cut(s) 299, 1109, 1183
BfuI GTATCC 1 cut(s) 1380
BlpI GCTNAGC 1 cut(s) 838
Bme1390I CCNGG 3 cut(s) 21, 844, 1485
Bme18I GGWCC 2 cut(s) 625, 1268
BmeT110I CYCGRG 3 cut(s) 134, 221, 266
BmgT120I GGNCC 3 cut(s) 582, 625, 1268
BmiI GGNNCC 3 cut(s) 627, 1270, 1536
BmrFI CCNGG 3 cut(s) 21, 844, 1485
BmsI GCATC 4 cut(s) 453, 846, 931, 1009
BpiI GAAGAC 2 cut(s) 749, 1308
BpmI CTGGAG 9 cut(s) 209, 423, 433, 530, 569, 620, 1146, 1263, 1506
Bpu1102I GCTNAGC 1 cut(s) 838
BpuEI CTTGAG 3 cut(s) 284, 359, 1157
BpuMI CCSGG 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 206
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 5 cut(s) 201, 681, 1284, 1360, 1483
BsaWI WCCGGW 1 cut(s) 613
Bsc4I CCNNNNNNNGG 7 cut(s) 20, 106, 187, 635, 699, 1361, 1472
Bse1I ACTGG 3 cut(s) 552, 1223, 1366
BseBI CCWGG 2 cut(s) 844, 1485
BseDI CCNNGG 5 cut(s) 201, 681, 1284, 1360, 1483
BseGI GGATG 2 cut(s) 811, 1355
BseLI CCNNNNNNNGG 7 cut(s) 20, 106, 187, 635, 699, 1361, 1472
BseMII CTCAG 4 cut(s) 24, 150, 443, 852
BseNI ACTGG 3 cut(s) 552, 1223, 1366
BseRI GAGGAG 5 cut(s) 89, 568, 731, 815, 1455
BseSI GKGCMC 2 cut(s) 759, 978
BseYI CCCAGC 4 cut(s) 579, 1064, 1234, 1376
BsgI GTGCAG 1 cut(s) 76
Bsh1236I CGCG 4 cut(s) 231, 263, 661, 678
BshFI GGCC 9 cut(s) 164, 318, 584, 692, 707, 882, 1222, 1289, 1365
BshNI GGYRCC 1 cut(s) 1534
BsiHKAI GWGCWC 5 cut(s) 114, 222, 759, 978, 1492
BsiHKCI CYCGRG 3 cut(s) 134, 221, 266
BsiSI CCGG 5 cut(s) 21, 494, 614, 1290, 1473
BslFI GGGAC 5 cut(s) 115, 260, 942, 1217, 1254
BslI CCNNNNNNNGG 7 cut(s) 20, 106, 187, 635, 699, 1361, 1472
BsmAI GTCTC 5 cut(s) 191, 538, 689, 1142, 1356
BsmFI GGGAC 5 cut(s) 115, 260, 942, 1217, 1254
BsmI GAATGC 1 cut(s) 1309
BsnI GGCC 9 cut(s) 164, 318, 584, 692, 707, 882, 1222, 1289, 1365
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 3 cut(s) 134, 221, 266
Bsp1286I GDGCHC 5 cut(s) 114, 222, 759, 978, 1492
Bsp143I GATC 9 cut(s) 9, 16, 240, 258, 490, 633, 918, 1051, 1475
Bsp1720I GCTNAGC 1 cut(s) 838
Bsp19I CCATGG 1 cut(s) 1360
BspACI CCGC 8 cut(s) 44, 67, 293, 319, 661, 726, 944, 1506
BspANI GGCC 9 cut(s) 164, 318, 584, 692, 707, 882, 1222, 1289, 1365
BspCNI CTCAG 4 cut(s) 25, 151, 444, 851
BspFNI CGCG 4 cut(s) 231, 263, 661, 678
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 627, 1270, 1536
BspMAI CTGCAG 2 cut(s) 771, 1036
BspPI GGATC 5 cut(s) 4, 11, 485, 1046, 1483
BspQI GCTCTTC 1 cut(s) 275
BspT107I GGYRCC 1 cut(s) 1534
BspTNI GGTCTC 1 cut(s) 191
BsrI ACTGG 3 cut(s) 552, 1223, 1366
BssECI CCNNGG 5 cut(s) 201, 681, 1284, 1360, 1483
BssMI GATC 9 cut(s) 9, 16, 240, 258, 490, 633, 918, 1051, 1475
BssNI GRCGYC 1 cut(s) 206
BssSI CACGAG 1 cut(s) 1251
BssT1I CCWWGG 3 cut(s) 201, 1284, 1360
Bst2BI CACGAG 1 cut(s) 1251
Bst2UI CCWGG 2 cut(s) 844, 1485
Bst4CI ACNGT 3 cut(s) 1265, 1389, 1529
Bst6I CTCTTC 2 cut(s) 275, 305
BstACI GRCGYC 1 cut(s) 206
BstC8I GCNNGC 6 cut(s) 586, 709, 851, 867, 1071, 1551
BstDEI CTNAG 6 cut(s) 33, 159, 452, 838, 1023, 1144
BstDSI CCRYGG 2 cut(s) 681, 1360
BstENI CCTNNNNNAGG 1 cut(s) 104
BstF5I GGATG 2 cut(s) 811, 1355
BstFNI CGCG 4 cut(s) 231, 263, 661, 678
BstH2I RGCGCY 3 cut(s) 299, 1109, 1183
BstHHI GCGC 7 cut(s) 231, 265, 298, 680, 849, 1108, 1182
BstKTI GATC 9 cut(s) 12, 19, 243, 261, 493, 636, 921, 1054, 1478
BstMAI GTCTC 5 cut(s) 191, 538, 689, 1142, 1356
BstMBI GATC 9 cut(s) 9, 16, 240, 258, 490, 633, 918, 1051, 1475
BstNI CCWGG 2 cut(s) 844, 1485
BstSCI CCNGG 3 cut(s) 19, 842, 1483
BstSFI CTRYAG 2 cut(s) 767, 1032
BstSLI GKGCMC 2 cut(s) 759, 978
BstUI CGCG 4 cut(s) 231, 263, 661, 678
BstV2I GAAGAC 2 cut(s) 749, 1308
BstX2I RGATCY 1 cut(s) 1051
BstXI CCANNNNNNTGG 1 cut(s) 688
BstYI RGATCY 1 cut(s) 1051
BsuI GTATCC 1 cut(s) 1380
BsuRI GGCC 9 cut(s) 164, 318, 584, 692, 707, 882, 1222, 1289, 1365
BtgI CCRYGG 2 cut(s) 681, 1360
BtsCI GGATG 2 cut(s) 811, 1355
Cac8I GCNNGC 6 cut(s) 586, 709, 851, 867, 1071, 1551
CaiI CAGNNNCTG 1 cut(s) 187
CciI TCATGA 1 cut(s) 81
CfoI GCGC 7 cut(s) 231, 265, 298, 680, 849, 1108, 1182
Cfr13I GGNCC 3 cut(s) 582, 625, 1268
CseI GACGC 4 cut(s) 195, 667, 1098, 1302
Csp6I GTAC 3 cut(s) 334, 1390, 1535
CviAII CATG 5 cut(s) 82, 1273, 1348, 1361, 1408
CviQI GTAC 3 cut(s) 334, 1390, 1535
DdeI CTNAG 6 cut(s) 33, 159, 452, 838, 1023, 1144
DpnI GATC 9 cut(s) 11, 18, 242, 260, 492, 635, 920, 1053, 1477
DpnII GATC 9 cut(s) 9, 16, 240, 258, 490, 633, 918, 1051, 1475
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 2 cut(s) 690, 1363
Eam1104I CTCTTC 2 cut(s) 275, 305
EarI CTCTTC 2 cut(s) 275, 305
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 3 cut(s) 112, 220, 1490
Eco130I CCWWGG 3 cut(s) 201, 1284, 1360
Eco24I GRGCYC 3 cut(s) 114, 222, 1492
Eco31I GGTCTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 519
Eco47I GGWCC 2 cut(s) 625, 1268
Eco47III AGCGCT 1 cut(s) 1107
Eco53kI GAGCTC 3 cut(s) 112, 220, 1490
Eco57I CTGAAG 1 cut(s) 170
Eco88I CYCGRG 3 cut(s) 134, 221, 266
EcoICRI GAGCTC 3 cut(s) 112, 220, 1490
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 842, 1483
EcoRV GATATC 1 cut(s) 519
EcoT14I CCWWGG 3 cut(s) 201, 1284, 1360
EcoT22I ATGCAT 2 cut(s) 1309, 1444
EcoT38I GRGCYC 3 cut(s) 114, 222, 1492
ErhI CCWWGG 3 cut(s) 201, 1284, 1360
FaeI CATG 5 cut(s) 85, 1276, 1351, 1364, 1411
FalI AAGNNNNNCTT 4 cut(s) 695, 727, 954, 986
FaqI GGGAC 5 cut(s) 115, 260, 942, 1217, 1254
FatI CATG 5 cut(s) 81, 1272, 1347, 1360, 1407
FauI CCCGC 1 cut(s) 1513
FauNDI CATATG 1 cut(s) 1309
FbaI TGATCA 1 cut(s) 918
FokI GGATG 2 cut(s) 818, 1342
FriOI GRGCYC 3 cut(s) 114, 222, 1492
FspBI CTAG 2 cut(s) 1082, 1193
GlaI GCGC 7 cut(s) 230, 264, 297, 679, 848, 1107, 1181
GsaI CCCAGC 4 cut(s) 583, 1068, 1238, 1380
GsuI CTGGAG 9 cut(s) 209, 423, 433, 530, 569, 620, 1146, 1263, 1506
HaeII RGCGCY 3 cut(s) 299, 1109, 1183
HaeIII GGCC 9 cut(s) 164, 318, 584, 692, 707, 882, 1222, 1289, 1365
HapII CCGG 5 cut(s) 21, 494, 614, 1290, 1473
HgaI GACGC 4 cut(s) 195, 667, 1098, 1302
HhaI GCGC 7 cut(s) 231, 265, 298, 680, 849, 1108, 1182
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 5 cut(s) 85, 1276, 1351, 1364, 1411
Hin6I GCGC 7 cut(s) 229, 263, 296, 678, 847, 1106, 1180
HinP1I GCGC 7 cut(s) 229, 263, 296, 678, 847, 1106, 1180
HincII GTYRAC 2 cut(s) 30, 39
HindII GTYRAC 2 cut(s) 30, 39
HindIII AAGCTT 2 cut(s) 824, 1005
HinfI GANTC 7 cut(s) 40, 184, 370, 398, 602, 610, 745
HpaII CCGG 5 cut(s) 21, 494, 614, 1290, 1473
HphI GGTGA 5 cut(s) 667, 790, 946, 1213, 1377
Hpy166II GTNNAC 7 cut(s) 30, 39, 346, 538, 625, 757, 976
Hpy188I TCNGA 6 cut(s) 118, 160, 453, 523, 1014, 1494
Hpy8I GTNNAC 7 cut(s) 30, 39, 346, 538, 625, 757, 976
Hpy99I CGWCG 5 cut(s) 59, 211, 218, 329, 353
HpyAV CCTTC 7 cut(s) 100, 265, 430, 697, 790, 1021, 1192
HpyCH4III ACNGT 3 cut(s) 1265, 1389, 1529
HpyCH4IV ACGT 2 cut(s) 738, 761
HpyF3I CTNAG 6 cut(s) 33, 159, 452, 838, 1023, 1144
HpySE526I ACGT 2 cut(s) 738, 761
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 5 cut(s) 85, 1276, 1351, 1364, 1411
HspAI GCGC 7 cut(s) 229, 263, 296, 678, 847, 1106, 1180
KpnI GGTACC 1 cut(s) 1538
Ksp22I TGATCA 1 cut(s) 918
Kzo9I GATC 9 cut(s) 9, 16, 240, 258, 490, 633, 918, 1051, 1475
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 6 cut(s) 109, 190, 555, 663, 1487, 1495
LweI GCATC 4 cut(s) 453, 846, 931, 1009
MaeI CTAG 2 cut(s) 1082, 1193
MaeII ACGT 2 cut(s) 738, 761
MaeIII GTNAC 4 cut(s) 235, 421, 655, 778
MalI GATC 9 cut(s) 11, 18, 242, 260, 492, 635, 920, 1053, 1477
MboI GATC 9 cut(s) 9, 16, 240, 258, 490, 633, 918, 1051, 1475
MflI RGATCY 1 cut(s) 1051
MhlI GDGCHC 5 cut(s) 114, 222, 759, 978, 1492
MlsI TGGCCA 1 cut(s) 1365
MluCI AATT 3 cut(s) 440, 914, 1154
MluNI TGGCCA 1 cut(s) 1365
MlyI GAGTC 4 cut(s) 34, 178, 611, 739
MmeI TCCRAC 2 cut(s) 141, 946
Mox20I TGGCCA 1 cut(s) 1365
Mph1103I ATGCAT 2 cut(s) 1309, 1444
MscI TGGCCA 1 cut(s) 1365
MslI CAYNNNNRTG 1 cut(s) 686
Msp20I TGGCCA 1 cut(s) 1365
MspA1I CMGCKG 2 cut(s) 67, 90
MspI CCGG 5 cut(s) 21, 494, 614, 1290, 1473
MspR9I CCNGG 3 cut(s) 21, 844, 1485
Mva1269I GAATGC 1 cut(s) 1309
MvaI CCWGG 2 cut(s) 844, 1485
MvnI CGCG 4 cut(s) 231, 263, 661, 678
NciI CCSGG 1 cut(s) 21
NcoI CCATGG 1 cut(s) 1360
NdeI CATATG 1 cut(s) 1309
NdeII GATC 9 cut(s) 9, 16, 240, 258, 490, 633, 918, 1051, 1475
NlaIII CATG 5 cut(s) 85, 1276, 1351, 1364, 1411
NlaIV GGNNCC 3 cut(s) 627, 1270, 1536
NmeAIII GCCGAG 2 cut(s) 72, 718
NmuCI GTSAC 4 cut(s) 235, 421, 655, 778
NsiI ATGCAT 2 cut(s) 1309, 1444
PaeR7I CTCGAG 2 cut(s) 134, 221
PagI TCATGA 1 cut(s) 81
PciSI GCTCTTC 1 cut(s) 275
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 228, 273
PctI GAATGC 1 cut(s) 1309
PfeI GAWTC 3 cut(s) 370, 398, 610
PflMI CCANNNNNTGG 2 cut(s) 187, 1361
PleI GAGTC 4 cut(s) 34, 178, 610, 739
PpsI GAGTC 4 cut(s) 34, 178, 610, 739
PsiI TTATAA 1 cut(s) 1140
Psp124BI GAGCTC 3 cut(s) 114, 222, 1492
Psp6I CCWGG 2 cut(s) 842, 1483
PspFI CCCAGC 4 cut(s) 579, 1064, 1234, 1376
PspGI CCWGG 2 cut(s) 842, 1483
PspN4I GGNNCC 3 cut(s) 627, 1270, 1536
PspPI GGNCC 3 cut(s) 582, 625, 1268
PspXI VCTCGAGB 2 cut(s) 134, 221
PstI CTGCAG 2 cut(s) 771, 1036
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 1051
PvuII CAGCTG 1 cut(s) 90
RsaI GTAC 3 cut(s) 335, 1391, 1536
RsaNI GTAC 3 cut(s) 334, 1390, 1535
RseI CAYNNNNRTG 1 cut(s) 686
SacI GAGCTC 3 cut(s) 114, 222, 1492
SapI GCTCTTC 1 cut(s) 275
Sau3AI GATC 9 cut(s) 9, 16, 240, 258, 490, 633, 918, 1051, 1475
Sau96I GGNCC 3 cut(s) 582, 625, 1268
SchI GAGTC 4 cut(s) 34, 178, 611, 739
ScrFI CCNGG 3 cut(s) 21, 844, 1485
SduI GDGCHC 5 cut(s) 114, 222, 759, 978, 1492
SfaNI GCATC 4 cut(s) 453, 846, 931, 1009
SfcI CTRYAG 2 cut(s) 767, 1032
Sfr274I CTCGAG 2 cut(s) 134, 221
SinI GGWCC 2 cut(s) 625, 1268
SlaI CTCGAG 2 cut(s) 134, 221
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 5 cut(s) 134, 221, 299, 374, 1172
SmoI CTYRAG 5 cut(s) 134, 221, 299, 374, 1172
Sse9I AATT 3 cut(s) 440, 914, 1154
SsiI CCGC 8 cut(s) 44, 67, 293, 319, 661, 726, 944, 1506
SspMI CTAG 2 cut(s) 1082, 1193
SstI GAGCTC 3 cut(s) 114, 222, 1492
StyD4I CCNGG 3 cut(s) 19, 842, 1483
StyI CCWWGG 3 cut(s) 201, 1284, 1360
TaaI ACNGT 3 cut(s) 1265, 1389, 1529
TaiI ACGT 2 cut(s) 741, 764
TaqI TCGA 8 cut(s) 8, 54, 135, 213, 222, 255, 276, 489
TasI AATT 3 cut(s) 440, 914, 1154
TauI GCSGC 4 cut(s) 70, 296, 321, 729
TfiI GAWTC 3 cut(s) 370, 398, 610
TseFI GTSAC 4 cut(s) 235, 421, 655, 778
Tsp45I GTSAC 4 cut(s) 235, 421, 655, 778
TspDTI ATGAA 3 cut(s) 383, 975, 1511
TspGWI ACGGA 1 cut(s) 698
Van91I CCANNNNNTGG 2 cut(s) 187, 1361
VneI GTGCAC 2 cut(s) 755, 974
VpaK11BI GGWCC 2 cut(s) 625, 1268
XagI CCTNNNNNAGG 1 cut(s) 104
XapI RAATTY 1 cut(s) 1154
XcmI CCANNNNNNNNNTGG 1 cut(s) 1373
XhoI CTCGAG 2 cut(s) 134, 221
XspI CTAG 2 cut(s) 1082, 1193
Zsp2I ATGCAT 2 cut(s) 1309, 1444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.