pycom16g16380

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010357
Physical Location & Seq
Forward (+)
12314219 .. 12314713
495 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16380.1

Sequence Viewer

Length: 495 bp
ATGCAGAACCTAGCTGGACGCAAAGAGCTGTCAGCGCTTCGGGCTGTCGTCAAGTGCATTGAAGATTATAACTTAGAGACAGAATTTCCTCCAGAAAACCTCAAGAAGCGCCTTGAACAGCTAGAGAAGGTGAAACCAGAGAAGAAAAGGCCAGTTGTAGTCCCAGCCAACAAGCGAACACGAGCGAACAACGGTGGTCCCATGCCTCCAGCCAAGGCAGGACGCTTGATGAATGCATATGTGTCTTCTTTCCCTACAACTCCTCCTACATTTGTCATGTCTCCATCCCATGGCCAGTATCCTGCTGGGTTCTCACCGTACCATTCCCCACCCACCATGTATGGCAGCAGAAGCCCACCAACCCCTTATGCATACTCGCCTGAAGCAGCCCCTCCTCCTGCCGGATCATACCCTGGAGCTCCGATGAACTATCCCGCCTATGGGGCTTATGGCAACGGTATGGTACCAGCTTATCAGCAGGCTTACTACCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

165

Amino Acids

17.98

Weight (kDa)

9.63

Isoelectric Point (pI)

76.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 2 - 51 7.3e-15 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 69
Acc65I GGTACC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 463
AccB7I CCANNNNNTGG 1 cut(s) 290
AciI CCGC 1 cut(s) 435
AclWI GGATC 1 cut(s) 412
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 1 cut(s) 83
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 2 cut(s) 320, 465
AfeI AGCGCT 1 cut(s) 36
AfiI CCNNNNNNNGG 4 cut(s) 290, 401, 440, 441
AgsI TTSAA 2 cut(s) 62, 116
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 5 cut(s) 14, 28, 121, 419, 470
AluI AGCT 5 cut(s) 14, 28, 121, 419, 470
Alw21I GWGCWC 1 cut(s) 421
Alw26I GTCTC 2 cut(s) 71, 285
AlwI GGATC 1 cut(s) 412
Aor51HI AGCGCT 1 cut(s) 36
AoxI GGCC 2 cut(s) 149, 292
ApeKI GCWGC 2 cut(s) 345, 386
ApoI RAATTY 1 cut(s) 83
Asp718I GGTACC 1 cut(s) 463
AspLEI GCGC 2 cut(s) 37, 111
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 142, 306
AvaII GGWCC 1 cut(s) 197
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 421
BauI CACGAG 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 357, 398
BccI CCATC 1 cut(s) 292
BciT130I CCWGG 1 cut(s) 414
BciVI GTATCC 1 cut(s) 309
BcoDI GTCTC 2 cut(s) 71, 285
BfaI CTAG 2 cut(s) 11, 122
BfoI RGCGCY 2 cut(s) 38, 112
BfuI GTATCC 1 cut(s) 309
BglI GCCNNNNNGGC 1 cut(s) 443
BisI GCNGC 2 cut(s) 346, 387
BlsI GCNGC 2 cut(s) 347, 388
Bme1390I CCNGG 1 cut(s) 414
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 199, 465
BmrFI CCNGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 237
BpmI CTGGAG 3 cut(s) 75, 192, 435
BpuEI CTTGAG 1 cut(s) 86
BsaJI CCNNGG 3 cut(s) 213, 289, 412
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 401, 440, 441
Bse1I ACTGG 2 cut(s) 152, 295
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 3 cut(s) 213, 289, 412
BseGI GGATG 1 cut(s) 284
BseLI CCNNNNNNNGG 4 cut(s) 290, 401, 440, 441
BseNI ACTGG 2 cut(s) 152, 295
BseRI GAGGAG 2 cut(s) 252, 384
BseXI GCAGC 2 cut(s) 357, 398
BseYI CCCAGC 2 cut(s) 163, 305
BshFI GGCC 2 cut(s) 151, 294
BshNI GGYRCC 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 421
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 2 cut(s) 146, 183
BslI CCNNNNNNNGG 4 cut(s) 290, 401, 440, 441
BsmAI GTCTC 2 cut(s) 71, 285
BsmFI GGGAC 2 cut(s) 146, 183
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 2 cut(s) 151, 294
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 1 cut(s) 404
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 1 cut(s) 435
BspANI GGCC 2 cut(s) 151, 294
BspLI GGNNCC 2 cut(s) 199, 465
BspPI GGATC 1 cut(s) 412
BspT107I GGYRCC 1 cut(s) 463
BsrI ACTGG 2 cut(s) 152, 295
BssECI CCNNGG 3 cut(s) 213, 289, 412
BssMI GATC 1 cut(s) 404
BssSI CACGAG 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 213, 289
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 3 cut(s) 194, 318, 458
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 1 cut(s) 73
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 1 cut(s) 284
BstH2I RGCGCY 2 cut(s) 38, 112
BstHHI GCGC 2 cut(s) 37, 111
BstKTI GATC 1 cut(s) 407
BstMAI GTCTC 2 cut(s) 71, 285
BstMBI GATC 1 cut(s) 404
BstMWI GCNNNNNNNGC 4 cut(s) 34, 41, 351, 443
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 412
BstV1I GCAGC 2 cut(s) 357, 398
BstV2I GAAGAC 1 cut(s) 237
BsuI GTATCC 1 cut(s) 309
BsuRI GGCC 2 cut(s) 151, 294
BtgI CCRYGG 1 cut(s) 289
BtsCI GGATG 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 480
CfoI GCGC 2 cut(s) 37, 111
Cfr13I GGNCC 1 cut(s) 197
CseI GACGC 2 cut(s) 27, 231
Csp6I GTAC 2 cut(s) 319, 464
CviAII CATG 4 cut(s) 202, 277, 290, 337
CviQI GTAC 2 cut(s) 319, 464
DdeI CTNAG 1 cut(s) 73
DpnI GATC 1 cut(s) 406
DpnII GATC 1 cut(s) 404
EaeI YGGCCR 1 cut(s) 292
Ecl136II GAGCTC 1 cut(s) 419
Eco130I CCWWGG 2 cut(s) 213, 289
Eco24I GRGCYC 1 cut(s) 421
Eco47I GGWCC 1 cut(s) 197
Eco47III AGCGCT 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 402
EcoICRI GAGCTC 1 cut(s) 419
EcoRII CCWGG 1 cut(s) 412
EcoT14I CCWWGG 2 cut(s) 213, 289
EcoT22I ATGCAT 2 cut(s) 238, 373
EcoT38I GRGCYC 1 cut(s) 421
ErhI CCWWGG 2 cut(s) 213, 289
FaeI CATG 4 cut(s) 205, 280, 293, 340
FaqI GGGAC 2 cut(s) 146, 183
FatI CATG 4 cut(s) 201, 276, 289, 336
FauI CCCGC 1 cut(s) 442
FauNDI CATATG 1 cut(s) 238
Fnu4HI GCNGC 2 cut(s) 346, 387
FokI GGATG 1 cut(s) 271
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 346, 387
FspBI CTAG 2 cut(s) 11, 122
GlaI GCGC 2 cut(s) 36, 110
GluI GCNGC 2 cut(s) 346, 387
GsaI CCCAGC 2 cut(s) 167, 309
GsuI CTGGAG 3 cut(s) 75, 192, 435
HaeII RGCGCY 2 cut(s) 38, 112
HaeIII GGCC 2 cut(s) 151, 294
HapII CCGG 1 cut(s) 402
HgaI GACGC 2 cut(s) 27, 231
HhaI GCGC 2 cut(s) 37, 111
Hin1II CATG 4 cut(s) 205, 280, 293, 340
Hin6I GCGC 2 cut(s) 35, 109
HinP1I GCGC 2 cut(s) 35, 109
HpaII CCGG 1 cut(s) 402
HphI GGTGA 2 cut(s) 142, 306
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 2 cut(s) 92, 103
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 3 cut(s) 194, 318, 458
HpyCH4V TGCA 4 cut(s) 4, 57, 236, 371
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 41, 351, 443
HpyF3I CTNAG 1 cut(s) 73
Hsp92II CATG 4 cut(s) 205, 280, 293, 340
HspAI GCGC 2 cut(s) 35, 109
KpnI GGTACC 1 cut(s) 467
Kzo9I GATC 1 cut(s) 404
LmnI GCTCC 2 cut(s) 416, 424
Lsp1109I GCAGC 2 cut(s) 357, 398
MaeI CTAG 2 cut(s) 11, 122
MalI GATC 1 cut(s) 406
MboI GATC 1 cut(s) 404
MboII GAAGA 3 cut(s) 74, 154, 237
MhlI GDGCHC 1 cut(s) 421
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 1 cut(s) 83
MluNI TGGCCA 1 cut(s) 294
MnlI CCTC 6 cut(s) 99, 110, 216, 273, 402, 405
Mox20I TGGCCA 1 cut(s) 294
Mph1103I ATGCAT 2 cut(s) 238, 373
MscI TGGCCA 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 294
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 1 cut(s) 414
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 4 cut(s) 34, 41, 351, 443
NcoI CCATGG 1 cut(s) 289
NdeI CATATG 1 cut(s) 238
NdeII GATC 1 cut(s) 404
NlaIII CATG 4 cut(s) 205, 280, 293, 340
NlaIV GGNNCC 2 cut(s) 199, 465
NsiI ATGCAT 2 cut(s) 238, 373
PctI GAATGC 1 cut(s) 238
PflMI CCANNNNNTGG 1 cut(s) 290
PkrI GCNGC 2 cut(s) 347, 388
PsiI TTATAA 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 412
PspFI CCCAGC 2 cut(s) 163, 305
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 199, 465
PspPI GGNCC 1 cut(s) 197
RsaI GTAC 2 cut(s) 320, 465
RsaNI GTAC 2 cut(s) 319, 464
SacI GAGCTC 1 cut(s) 421
SatI GCNGC 2 cut(s) 346, 387
Sau3AI GATC 1 cut(s) 404
Sau96I GGNCC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 1 cut(s) 421
SetI ASST 8 cut(s) 12, 16, 30, 102, 123, 132, 421, 472
SinI GGWCC 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 1 cut(s) 83
SsiI CCGC 1 cut(s) 435
SspMI CTAG 2 cut(s) 11, 122
SstI GAGCTC 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 412
StyI CCWWGG 2 cut(s) 213, 289
TaaI ACNGT 3 cut(s) 194, 318, 458
TasI AATT 1 cut(s) 83
TseI GCWGC 2 cut(s) 345, 386
TspDTI ATGAA 2 cut(s) 245, 440
Van91I CCANNNNNTGG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 197
XapI RAATTY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XspI CTAG 2 cut(s) 11, 122
Zsp2I ATGCAT 2 cut(s) 238, 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.