pycom13g16540

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010357
Physical Location & Seq
Forward (+)
12358772 .. 12361753
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16540.1

Sequence Viewer

Length: 1566 bp
ATGGGGTCGATCCCCGATCCCGGCGAGGTGACTGAGTTGACTCCGCCGAGCTTCGACGAGTTCCAGCGGCAGACTTCTCTCATGACCAGCTGCACTCTCCTCTGGAAGGAGCTCTCCGACCACTTCTCGTCCCTCGAGCAGAACCTCCTGCAGAAATCCGAGGCCATCAAGCTCAAAATCCAGACCCTGGAGCACGAGACCAAGGCCTCGCTCGACGAGCTCGAGCAGCGCGAGGTCTCGATCAGGGACATCTTCGAAGTCGCGCTTGGCAAGGTCGAGAAGAGCAAGGAGGCGGCGCTCAAGGTGTTGAAGAGAGCCCGCAGCGATGACGAGTACGGGGAGGTGGACGACGGCGAGGGGTTGCTGATGAATCTCAAGTCGTTGTGCTTGAAGATGGATTCTGGAGGGTTCTGGAGGTTCGTGACGGCGAGGAAGAAGGAATTGGAGGCTCTGAGGTCGCAGATGCCTCTGGCTTTGGGTGATTGTGTCGATCCGGCGAAGTTCGTGCTGGAGGCGATATCGGAGGTGTTTCCGGTGGACAAGAGAGTGGAGAGGAGCGATAGGGGAAACGATTTGGGCTGGGCCTGCGTTCTGTTGCTGGAGTCACTGATTCCGGTGATGATGGACCCGAAGATCGGGAGAAAGAGGCTTCTGGTGACGCGGAGCGTGAAGCAACGCGCCACCGAGATATCCGAGACCTGGAAGGCCAGCTTGGAGGAGAGAGGCGGCATAGAGAACGTGAAGACTCCTGATGTGCACACGTTCCTGCAGCATTTGGTGACTTTCGGGATTGTGAAGGAGGAGGATGTTGAGTTGTACAGGAAGCTTGTGGTTAACTCAGCCTGGCGCAAGCAGATGCCGAAGCTCGCTGTTTCGCTTGGCCTCGCCAAGCAAATGCCGGATATGATTGAAGAATTGATCAGCAGGGGACAGCAGCTTGATGCGGTGCATTTCACCTATGAGGTTGGACTTGTGCACAAGTTTCCTCCTGTTCCTCTACTGAAAGCTTTTCTGAAGGATGCTAAGAAAGCTGCGGCTTCTATTTTGGAAGATCCCAATAACGCTGGGCGAGCTGCGAACCTAGCTGGACGCAAAGAGCTGTCAGCGCTCCGGGCTGTCATCAAGTGCATTGAAGATTATAACCTTGAGGCAGAATTTCCTCCAGAAGACCTCAAGAAGCGCCTTGAACAGCTAGAGAAGGTGAAACCAGAGAAGAAAAGGCCAGTTGTAGTCCCAGCCAACAAACGAGTACGAGCTAACAATGGCGGTCCCATGCCTCCGGCCAAGGCTGGACGCTTGACGAATGCATATGTGTCTTCTTTCCCTACACCTCCTACATTTGTCAGGTCTCCTTCCCATGGCCAGTATCCTGCTGGGTTCTCACCGTACCATTCCCCACCCACCATGTATGGTAGCAGAAGCCCACCAACCCCTTATGCATACTCACCGGAAGCAGCACCCCCTCCTCATGCCGGATCATACCCTGGACCTCCGATGAACTATCCCGCATATGGTGCTTATGGCAATGGTATGGTACCAGCTTATCAGCCGGCTTACTACCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

522

Amino Acids

58.08

Weight (kDa)

8.33

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 123 - 408 5.4e-106 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
Acc65I GGTACC 1 cut(s) 1534
AccB1I GGYRCC 1 cut(s) 1534
AccB7I CCANNNNNTGG 1 cut(s) 187
AccII CGCG 4 cut(s) 231, 263, 661, 678
AclWI GGATC 5 cut(s) 4, 11, 485, 1046, 1483
AcoI YGGCCR 2 cut(s) 1281, 1360
AcsI RAATTY 1 cut(s) 1154
AcuI CTGAAG 1 cut(s) 1034
AfaI GTAC 5 cut(s) 335, 818, 1251, 1388, 1536
AfeI AGCGCT 1 cut(s) 1107
AfiI CCNNNNNNNGG 8 cut(s) 20, 106, 187, 635, 699, 1358, 1472, 1511
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 5 cut(s) 310, 391, 911, 1133, 1187
AjnI CCWGG 4 cut(s) 186, 698, 842, 1483
AjuI GAANNNNNNNTTGG 6 cut(s) 249, 281, 425, 457, 695, 727
Alw21I GWGCWC 5 cut(s) 114, 195, 222, 759, 978
Alw26I GTCTC 4 cut(s) 191, 241, 689, 1353
Alw44I GTGCAC 2 cut(s) 755, 974
AlwI GGATC 5 cut(s) 4, 11, 485, 1046, 1483
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 2 cut(s) 134, 221
Aor51HI AGCGCT 1 cut(s) 1107
AoxI GGCC 8 cut(s) 162, 204, 582, 705, 880, 1220, 1281, 1360
ApaLI GTGCAC 2 cut(s) 755, 974
ApeKI GCWGC 8 cut(s) 90, 226, 321, 769, 934, 1031, 1073, 1454
ApoI RAATTY 1 cut(s) 1154
Asp718I GGTACC 1 cut(s) 1534
AspLEI GCGC 7 cut(s) 231, 265, 298, 680, 849, 1108, 1182
AspS9I GGNCC 4 cut(s) 582, 625, 1268, 1487
AsuC2I CCSGG 2 cut(s) 21, 1112
AsuHPI GGTGA 9 cut(s) 40, 491, 628, 667, 790, 946, 1213, 1374, 1437
AsuII TTCGAA 1 cut(s) 255
AvaI CYCGRG 2 cut(s) 134, 221
AvaII GGWCC 3 cut(s) 625, 1268, 1487
BaeGI GKGCMC 2 cut(s) 759, 978
BalI TGGCCA 1 cut(s) 1362
BanI GGYRCC 1 cut(s) 1534
BanII GRGCYC 3 cut(s) 114, 222, 319
BauI CACGAG 1 cut(s) 194
BbsI GAAGAC 3 cut(s) 749, 1173, 1308
Bbv12I GWGCWC 5 cut(s) 114, 195, 222, 759, 978
BbvI GCAGC 8 cut(s) 77, 238, 333, 781, 946, 1018, 1060, 1466
BccI CCATC 3 cut(s) 173, 388, 616
BceAI ACGGC 2 cut(s) 367, 441
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 4 cut(s) 188, 700, 844, 1485
BciVI GTATCC 1 cut(s) 1377
BclI TGATCA 1 cut(s) 918
BcnI CCSGG 2 cut(s) 21, 1112
BcoDI GTCTC 4 cut(s) 191, 241, 689, 1353
BfaI CTAG 2 cut(s) 1082, 1193
BfmI CTRYAG 2 cut(s) 149, 767
BfoI RGCGCY 3 cut(s) 299, 1109, 1183
BfuI GTATCC 1 cut(s) 1377
Bme1390I CCNGG 6 cut(s) 21, 188, 700, 844, 1112, 1485
Bme18I GGWCC 3 cut(s) 625, 1268, 1487
BmeT110I CYCGRG 2 cut(s) 134, 221
BmgT120I GGNCC 4 cut(s) 582, 625, 1268, 1487
BmiI GGNNCC 3 cut(s) 627, 1270, 1536
BmrFI CCNGG 6 cut(s) 21, 188, 700, 844, 1112, 1485
BmsI GCATC 4 cut(s) 453, 846, 931, 1009
BpiI GAAGAC 3 cut(s) 749, 1173, 1308
BpmI CTGGAG 6 cut(s) 209, 423, 433, 530, 620, 1146
Bpu14I TTCGAA 1 cut(s) 255
BpuEI CTTGAG 4 cut(s) 284, 359, 1157, 1166
BpuMI CCSGG 2 cut(s) 21, 1112
BsaI GGTCTC 4 cut(s) 191, 241, 689, 1353
BsaJI CCNNGG 6 cut(s) 159, 186, 201, 1284, 1357, 1483
BsaWI WCCGGW 3 cut(s) 532, 613, 1447
Bsc4I CCNNNNNNNGG 8 cut(s) 20, 106, 187, 635, 699, 1358, 1472, 1511
Bse118I RCCGGY 1 cut(s) 1549
Bse1I ACTGG 2 cut(s) 1223, 1363
Bse3DI GCAATG 1 cut(s) 1531
BseBI CCWGG 4 cut(s) 188, 700, 844, 1485
BseDI CCNNGG 6 cut(s) 159, 186, 201, 1284, 1357, 1483
BseGI GGATG 2 cut(s) 811, 1024
BseLI CCNNNNNNNGG 8 cut(s) 20, 106, 187, 635, 699, 1358, 1472, 1511
BseMI GCAATG 1 cut(s) 1531
BseMII CTCAG 3 cut(s) 24, 443, 852
BseNI ACTGG 2 cut(s) 1223, 1363
BseRI GAGGAG 5 cut(s) 89, 568, 731, 815, 1455
BseSI GKGCMC 2 cut(s) 759, 978
BseXI GCAGC 8 cut(s) 77, 238, 333, 781, 946, 1018, 1060, 1466
BseYI CCCAGC 4 cut(s) 579, 1064, 1234, 1373
BsgI GTGCAG 1 cut(s) 76
Bsh1236I CGCG 4 cut(s) 231, 263, 661, 678
BshFI GGCC 8 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1362
BshNI GGYRCC 1 cut(s) 1534
BsiHKAI GWGCWC 5 cut(s) 114, 195, 222, 759, 978
BsiHKCI CYCGRG 2 cut(s) 134, 221
BslFI GGGAC 5 cut(s) 115, 260, 942, 1217, 1254
BslI CCNNNNNNNGG 8 cut(s) 20, 106, 187, 635, 699, 1358, 1472, 1511
BsmAI GTCTC 4 cut(s) 191, 241, 689, 1353
BsmFI GGGAC 5 cut(s) 115, 260, 942, 1217, 1254
BsmI GAATGC 1 cut(s) 1309
BsnI GGCC 8 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1362
Bso31I GGTCTC 4 cut(s) 191, 241, 689, 1353
BsoBI CYCGRG 2 cut(s) 134, 221
Bsp119I TTCGAA 1 cut(s) 255
Bsp1286I GDGCHC 6 cut(s) 114, 195, 222, 319, 759, 978
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1475
Bsp19I CCATGG 1 cut(s) 1357
BspANI GGCC 8 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1362
BspCNI CTCAG 3 cut(s) 25, 444, 851
BspFNI CGCG 4 cut(s) 231, 263, 661, 678
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 627, 1270, 1536
BspMAI CTGCAG 2 cut(s) 153, 771
BspPI GGATC 5 cut(s) 4, 11, 485, 1046, 1483
BspQI GCTCTTC 1 cut(s) 275
BspT104I TTCGAA 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 1534
BspTNI GGTCTC 4 cut(s) 191, 241, 689, 1353
BsrDI GCAATG 1 cut(s) 1531
BsrFI RCCGGY 1 cut(s) 1549
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 2 cut(s) 1223, 1363
BssAI RCCGGY 1 cut(s) 1549
BssECI CCNNGG 6 cut(s) 159, 186, 201, 1284, 1357, 1483
BssMI GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1475
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 3 cut(s) 201, 1284, 1357
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 4 cut(s) 188, 700, 844, 1485
Bst4CI ACNGT 1 cut(s) 1386
Bst6I CTCTTC 2 cut(s) 275, 305
BstAPI GCANNNNNTGC 1 cut(s) 1514
BstAUI TGTACA 1 cut(s) 816
BstBI TTCGAA 1 cut(s) 255
BstC8I GCNNGC 7 cut(s) 319, 586, 709, 851, 867, 1071, 1551
BstDEI CTNAG 4 cut(s) 33, 452, 838, 1023
BstDSI CCRYGG 1 cut(s) 1357
BstENI CCTNNNNNAGG 1 cut(s) 104
BstF5I GGATG 2 cut(s) 811, 1024
BstFNI CGCG 4 cut(s) 231, 263, 661, 678
BstH2I RGCGCY 3 cut(s) 299, 1109, 1183
BstHHI GCGC 7 cut(s) 231, 265, 298, 680, 849, 1108, 1182
BstKTI GATC 8 cut(s) 12, 19, 243, 493, 636, 921, 1054, 1478
BstMAI GTCTC 4 cut(s) 191, 241, 689, 1353
BstMBI GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1475
BstMWI GCNNNNNNNGC 9 cut(s) 217, 226, 585, 1028, 1070, 1082, 1105, 1112, 1514
BstNI CCWGG 4 cut(s) 188, 700, 844, 1485
BstSCI CCNGG 6 cut(s) 19, 186, 698, 842, 1110, 1483
BstSFI CTRYAG 2 cut(s) 149, 767
BstSLI GKGCMC 2 cut(s) 759, 978
BstUI CGCG 4 cut(s) 231, 263, 661, 678
BstV1I GCAGC 8 cut(s) 77, 238, 333, 781, 946, 1018, 1060, 1466
BstV2I GAAGAC 3 cut(s) 749, 1173, 1308
BstX2I RGATCY 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 1051
BsuI GTATCC 1 cut(s) 1377
BsuRI GGCC 8 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1362
BtgI CCRYGG 1 cut(s) 1357
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 811, 1024
BtsIMutI CAGTG 1 cut(s) 605
Cac8I GCNNGC 7 cut(s) 319, 586, 709, 851, 867, 1071, 1551
CaiI CAGNNNCTG 1 cut(s) 187
CciI TCATGA 1 cut(s) 81
CfoI GCGC 7 cut(s) 231, 265, 298, 680, 849, 1108, 1182
Cfr10I RCCGGY 1 cut(s) 1549
Cfr13I GGNCC 4 cut(s) 582, 625, 1268, 1487
CseI GACGC 3 cut(s) 667, 1098, 1302
Csp6I GTAC 5 cut(s) 334, 817, 1250, 1387, 1535
CviAII CATG 5 cut(s) 82, 1273, 1358, 1405, 1469
CviQI GTAC 5 cut(s) 334, 817, 1250, 1387, 1535
DdeI CTNAG 4 cut(s) 33, 452, 838, 1023
DpnI GATC 8 cut(s) 11, 18, 242, 492, 635, 920, 1053, 1477
DpnII GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1475
EaeI YGGCCR 2 cut(s) 1281, 1360
Eam1104I CTCTTC 2 cut(s) 275, 305
EarI CTCTTC 2 cut(s) 275, 305
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 2 cut(s) 112, 220
Eco130I CCWWGG 3 cut(s) 201, 1284, 1357
Eco147I AGGCCT 1 cut(s) 206
Eco24I GRGCYC 3 cut(s) 114, 222, 319
Eco31I GGTCTC 4 cut(s) 191, 241, 689, 1353
Eco32I GATATC 2 cut(s) 519, 690
Eco47I GGWCC 3 cut(s) 625, 1268, 1487
Eco47III AGCGCT 1 cut(s) 1107
Eco53kI GAGCTC 2 cut(s) 112, 220
Eco57I CTGAAG 1 cut(s) 1034
Eco88I CYCGRG 2 cut(s) 134, 221
EcoICRI GAGCTC 2 cut(s) 112, 220
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoRII CCWGG 4 cut(s) 186, 698, 842, 1483
EcoRV GATATC 2 cut(s) 519, 690
EcoT14I CCWWGG 3 cut(s) 201, 1284, 1357
EcoT22I ATGCAT 2 cut(s) 1309, 1441
EcoT38I GRGCYC 3 cut(s) 114, 222, 319
ErhI CCWWGG 3 cut(s) 201, 1284, 1357
FaeI CATG 5 cut(s) 85, 1276, 1361, 1408, 1472
FalI AAGNNNNNCTT 4 cut(s) 249, 281, 695, 727
FaqI GGGAC 5 cut(s) 115, 260, 942, 1217, 1254
FatI CATG 5 cut(s) 81, 1272, 1357, 1404, 1468
FauI CCCGC 2 cut(s) 326, 1513
FauNDI CATATG 2 cut(s) 1309, 1510
FbaI TGATCA 1 cut(s) 918
FokI GGATG 2 cut(s) 818, 1031
FriOI GRGCYC 3 cut(s) 114, 222, 319
FspBI CTAG 2 cut(s) 1082, 1193
GlaI GCGC 7 cut(s) 230, 264, 297, 679, 848, 1107, 1181
GsaI CCCAGC 4 cut(s) 583, 1068, 1238, 1377
GsuI CTGGAG 6 cut(s) 209, 423, 433, 530, 620, 1146
HaeII RGCGCY 3 cut(s) 299, 1109, 1183
HaeIII GGCC 8 cut(s) 164, 206, 584, 707, 882, 1222, 1283, 1362
HgaI GACGC 3 cut(s) 667, 1098, 1302
HhaI GCGC 7 cut(s) 231, 265, 298, 680, 849, 1108, 1182
Hin1II CATG 5 cut(s) 85, 1276, 1361, 1408, 1472
Hin6I GCGC 7 cut(s) 229, 263, 296, 678, 847, 1106, 1180
HinP1I GCGC 7 cut(s) 229, 263, 296, 678, 847, 1106, 1180
HincII GTYRAC 2 cut(s) 39, 835
HindII GTYRAC 2 cut(s) 39, 835
HindIII AAGCTT 2 cut(s) 824, 1005
HinfI GANTC 6 cut(s) 40, 370, 398, 602, 610, 745
HpaI GTTAAC 1 cut(s) 835
HphI GGTGA 9 cut(s) 40, 491, 628, 667, 790, 946, 1213, 1374, 1437
Hpy166II GTNNAC 6 cut(s) 39, 346, 538, 757, 835, 976
Hpy188I TCNGA 7 cut(s) 118, 160, 453, 523, 694, 1014, 1494
Hpy8I GTNNAC 6 cut(s) 39, 346, 538, 757, 835, 976
Hpy99I CGWCG 3 cut(s) 59, 218, 353
HpyAV CCTTC 7 cut(s) 100, 430, 697, 790, 1009, 1192, 1362
HpyCH4III ACNGT 1 cut(s) 1386
HpyCH4IV ACGT 2 cut(s) 738, 761
HpyCH4V TGCA 9 cut(s) 93, 151, 757, 769, 949, 976, 1128, 1307, 1439
HpyF10VI GCNNNNNNNGC 9 cut(s) 217, 226, 585, 1028, 1070, 1082, 1105, 1112, 1514
HpyF3I CTNAG 4 cut(s) 33, 452, 838, 1023
HpySE526I ACGT 2 cut(s) 738, 761
Hsp92II CATG 5 cut(s) 85, 1276, 1361, 1408, 1472
HspAI GCGC 7 cut(s) 229, 263, 296, 678, 847, 1106, 1180
KpnI GGTACC 1 cut(s) 1538
KroI GCCGGC 1 cut(s) 1549
KroNI GCCGGC 1 cut(s) 1551
Ksp22I TGATCA 1 cut(s) 918
KspAI GTTAAC 1 cut(s) 835
Kzo9I GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1475
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 5 cut(s) 109, 190, 555, 663, 1113
Lsp1109I GCAGC 8 cut(s) 77, 238, 333, 781, 946, 1018, 1060, 1466
LweI GCATC 4 cut(s) 453, 846, 931, 1009
MaeI CTAG 2 cut(s) 1082, 1193
MaeII ACGT 2 cut(s) 738, 761
MaeIII GTNAC 5 cut(s) 28, 421, 603, 655, 778
MalI GATC 8 cut(s) 11, 18, 242, 492, 635, 920, 1053, 1477
MboI GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1475
MflI RGATCY 1 cut(s) 1051
MhlI GDGCHC 6 cut(s) 114, 195, 222, 319, 759, 978
MlsI TGGCCA 1 cut(s) 1362
MluCI AATT 3 cut(s) 440, 914, 1154
MluNI TGGCCA 1 cut(s) 1362
MlyI GAGTC 3 cut(s) 34, 611, 739
MmeI TCCRAC 2 cut(s) 141, 946
Mox20I TGGCCA 1 cut(s) 1362
Mph1103I ATGCAT 2 cut(s) 1309, 1441
MroNI GCCGGC 1 cut(s) 1549
MscI TGGCCA 1 cut(s) 1362
MseI TTAA 1 cut(s) 834
Msp20I TGGCCA 1 cut(s) 1362
MspA1I CMGCKG 2 cut(s) 67, 90
MspR9I CCNGG 6 cut(s) 21, 188, 700, 844, 1112, 1485
Mva1269I GAATGC 1 cut(s) 1309
MvaI CCWGG 4 cut(s) 188, 700, 844, 1485
MvnI CGCG 4 cut(s) 231, 263, 661, 678
MwoI GCNNNNNNNGC 9 cut(s) 217, 226, 585, 1028, 1070, 1082, 1105, 1112, 1514
NaeI GCCGGC 1 cut(s) 1551
NciI CCSGG 2 cut(s) 21, 1112
NcoI CCATGG 1 cut(s) 1357
NdeI CATATG 2 cut(s) 1309, 1510
NdeII GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1475
NgoMIV GCCGGC 1 cut(s) 1549
NlaIII CATG 5 cut(s) 85, 1276, 1361, 1408, 1472
NlaIV GGNNCC 3 cut(s) 627, 1270, 1536
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 5 cut(s) 28, 421, 603, 655, 778
NsiI ATGCAT 2 cut(s) 1309, 1441
NspV TTCGAA 1 cut(s) 255
PaeR7I CTCGAG 2 cut(s) 134, 221
PagI TCATGA 1 cut(s) 81
PceI AGGCCT 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 275
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 228
PctI GAATGC 1 cut(s) 1309
PdiI GCCGGC 1 cut(s) 1551
PfeI GAWTC 3 cut(s) 370, 398, 610
PflMI CCANNNNNTGG 1 cut(s) 187
PleI GAGTC 3 cut(s) 34, 610, 739
PpsI GAGTC 3 cut(s) 34, 610, 739
PsiI TTATAA 1 cut(s) 1140
Psp124BI GAGCTC 2 cut(s) 114, 222
Psp6I CCWGG 4 cut(s) 186, 698, 842, 1483
PspFI CCCAGC 4 cut(s) 579, 1064, 1234, 1373
PspGI CCWGG 4 cut(s) 186, 698, 842, 1483
PspN4I GGNNCC 3 cut(s) 627, 1270, 1536
PspPI GGNCC 4 cut(s) 582, 625, 1268, 1487
PspXI VCTCGAGB 2 cut(s) 134, 221
PstI CTGCAG 2 cut(s) 153, 771
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 1051
PvuII CAGCTG 1 cut(s) 90
RsaI GTAC 5 cut(s) 335, 818, 1251, 1388, 1536
RsaNI GTAC 5 cut(s) 334, 817, 1250, 1387, 1535
SacI GAGCTC 2 cut(s) 114, 222
SapI GCTCTTC 1 cut(s) 275
SaqAI TTAA 1 cut(s) 834
Sau3AI GATC 8 cut(s) 9, 16, 240, 490, 633, 918, 1051, 1475
Sau96I GGNCC 4 cut(s) 582, 625, 1268, 1487
SchI GAGTC 3 cut(s) 34, 611, 739
ScrFI CCNGG 6 cut(s) 21, 188, 700, 844, 1112, 1485
SduI GDGCHC 6 cut(s) 114, 195, 222, 319, 759, 978
SfaNI GCATC 4 cut(s) 453, 846, 931, 1009
SfcI CTRYAG 2 cut(s) 149, 767
Sfr274I CTCGAG 2 cut(s) 134, 221
SfuI TTCGAA 1 cut(s) 255
SinI GGWCC 3 cut(s) 625, 1268, 1487
SlaI CTCGAG 2 cut(s) 134, 221
SmlI CTYRAG 6 cut(s) 134, 221, 299, 374, 1145, 1172
SmoI CTYRAG 6 cut(s) 134, 221, 299, 374, 1145, 1172
Sse9I AATT 3 cut(s) 440, 914, 1154
SseBI AGGCCT 1 cut(s) 206
SspMI CTAG 2 cut(s) 1082, 1193
SstI GAGCTC 2 cut(s) 114, 222
StuI AGGCCT 1 cut(s) 206
StyD4I CCNGG 6 cut(s) 19, 186, 698, 842, 1110, 1483
StyI CCWWGG 3 cut(s) 201, 1284, 1357
TaaI ACNGT 1 cut(s) 1386
TaiI ACGT 2 cut(s) 741, 764
TaqI TCGA 9 cut(s) 8, 54, 135, 213, 222, 239, 255, 276, 489
TasI AATT 3 cut(s) 440, 914, 1154
TatI WGTACW 1 cut(s) 816
TauI GCSGC 4 cut(s) 70, 296, 729, 1037
TfiI GAWTC 3 cut(s) 370, 398, 610
Tru1I TTAA 1 cut(s) 834
Tru9I TTAA 1 cut(s) 834
TscAI CASTG 1 cut(s) 612
TseFI GTSAC 5 cut(s) 28, 421, 603, 655, 778
TseI GCWGC 8 cut(s) 90, 226, 321, 769, 934, 1031, 1073, 1454
Tsp45I GTSAC 5 cut(s) 28, 421, 603, 655, 778
TspDTI ATGAA 2 cut(s) 383, 1511
TspRI CASTG 1 cut(s) 612
Van91I CCANNNNNTGG 1 cut(s) 187
VneI GTGCAC 2 cut(s) 755, 974
VpaK11BI GGWCC 3 cut(s) 625, 1268, 1487
XagI CCTNNNNNAGG 1 cut(s) 104
XapI RAATTY 1 cut(s) 1154
XcmI CCANNNNNNNNNTGG 1 cut(s) 1370
XhoI CTCGAG 2 cut(s) 134, 221
XspI CTAG 2 cut(s) 1082, 1193
Zsp2I ATGCAT 2 cut(s) 1309, 1441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.