Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004270

Physical Location & Seq

Genomic Coordinates Forward (+)

20415790 .. 20423508

Gene Structure 7719 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1215300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1062 bp

ATGAAAAGTTTGCAGGAGAGGAAGGAGCAGTATAAGTTGGCTGATGCTGATGTGTCTGAGGAAGAGCAAAACAACTTGCTAAAGTACTTGGAGAAGAAAGAAACAGAATATATGCGTATTCAGAGGCATAAAATGGGTGCTGATGATTTTGAACCACTGACAATGATAGGAAAGGGTGCATTTGGAGAGGTTAGAATCTGTAGGGAGAAAGCAACTGGTCACGTATATGCAATGAAGAAGCTTAAGAAATCAGATATGCTTCGGAGAGGCCAGGTGGAACATGTGAAAGCTGAGAGGAATCTACTTGCTGAGGTTGACAGTAATTGTATCGTCAAGCTCTATTGTTCGTTCCAGGATGAAGAGTACTTGTATCTAATTATGGAATATCTTCCTGGTGGGGATATGATGACATTATTGATGCGCAAGGATACATTGACAGAATATGAAGCTATATTTTATATTGGGGAAACCGTCCTAGCTATTGAGTCCATTCATAAACACAATTACATCCATAGAGATATTAAGCCTGACAATTTGCTGCTGGATAGAAATGGTCACATGAAATTATCAGATTTTGGATTATGCAAACCATTAGATTGTAGTATACTTCAAGAAAAGGATTTTTCTGTGGGAAAAAATCTTAGCGGGGCTCTTCAAAGCAATGGACGGCCTGCACCACCAAAGCGTACACAACAGGAGCAACTGCAGCATTGGCAGAGAAACCGGAGGATGCTTGCTTATTCTACGGTTGGAACCCCAGACTATATTGCCCCAGAAGTTTTGCTGAAGAAAGGATATGGAATGGAATGTGATTGGTGGTCCCTTGGGGCTATTATGTACGAAATGCTTGTGGGATATCCACCCTTTTATTCAGATGACCCCATGTCAACTTGTAGAAAGATAGTTAATTGGAGAACTCATCTGAAATTTCCTGAAGAAGCCAAACTCTCTCCGGAAGCCAAGGATCTCATTAGTAAACTCTTATGTAATGTTGACCAGAGGCTTGGAACAAAAGGGGCAGATGAAATAAAGGAATTGAATGGGACAAATTGTATCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0010975 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0022008 GO:0023052 GO:0030154 GO:0030554 GO:0031344 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0045664 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050773 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051716 GO:0051960 GO:0060284 GO:0065007 GO:0071704 GO:0097159 GO:0097367 GO:0120035 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_C

Protein Analysis

354

Amino Acids

40.84

Weight (kDa)

6.77

Isoelectric Point (pI)

36.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	52 - 199	1.6e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	54 - 198	3.2e-23	Protein tyrosine and serine/threonine kinase
ABC1	PF03109	124 - 199	2.4e-06	ABC1 atypical kinase-like domain
Pkinase	PF00069	246 - 336	1.7e-20	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004270)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23310 AT4G14350 AT4G14350 AT4G14350 AT4G14350
fragaria_vesca	FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790
malus_domestica	MD13G1215300.v1.1 MD16G1220500.v1.1
prunus_persica	Prupe.1G040500_v2.0.a1 Prupe.1G040500_v2.0.a1 Prupe.1G040500_v2.0.a1
pyrus_communis	pycom13g18850 pycom16g18470
rosa_chinensis	RchiOBHm_Chr4g0393641
rosa_laevigata	RLG00000009739
rosa_multiflora	Rmu_sc0006302.1_g000002
rosa_roxburghii	Rroxscaffold_5G00338810
rosa_rugosa	Rorug03G0372000 Rorug03G0372100 Rorug03G0372200 Rorug03G0372300.1
rosa_samantha	Rh4AG048200 Rh4BG044700 Rh4CG051500 Rh4DG045500
rosa_wichuraiana	Rw4G003820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	422
AccI	GTMKAC	1 cut(s)	604
AccIII	TCCGGA	1 cut(s)	952
AciI	CCGC	1 cut(s)	645
AclWI	GGATC	1 cut(s)	972
AcsI	RAATTY	1 cut(s)	926
AcuI	CTGAAG	2 cut(s)	806, 954
AfaI	GTAC	4 cut(s)	86, 365, 688, 839
AflII	CTTAAG	1 cut(s)	242
AflIII	ACRYGT	1 cut(s)	280
AgsI	TTSAA	4 cut(s)	152, 611, 656, 1039
AhdI	GACNNNNNGTC	1 cut(s)	883
AjnI	CCWGG	3 cut(s)	270, 351, 391
AluBI	AGCT	5 cut(s)	241, 290, 337, 449, 479
AluI	AGCT	5 cut(s)	241, 290, 337, 449, 479
AlwI	GGATC	1 cut(s)	972
Aor13HI	TCCGGA	1 cut(s)	952
AoxI	GGCC	2 cut(s)	268, 668
ApeKI	GCWGC	2 cut(s)	538, 706
ApoI	RAATTY	1 cut(s)	926
Asp700I	GAANNNNTTC	1 cut(s)	387
AspLEI	GCGC	1 cut(s)	423
AspS9I	GGNCC	1 cut(s)	819
AvaII	GGWCC	1 cut(s)	819
BanII	GRGCYC	1 cut(s)	652
BbvCI	CCTCAGC	1 cut(s)	309
BbvI	GCAGC	2 cut(s)	525, 718
BceAI	ACGGC	1 cut(s)	683
BciT130I	CCWGG	3 cut(s)	272, 353, 393
BciVI	GTATCC	1 cut(s)	421
BfaI	CTAG	1 cut(s)	476
BfmI	CTRYAG	2 cut(s)	199, 704
BfrI	CTTAAG	1 cut(s)	242
BfuI	GTATCC	1 cut(s)	421
BisI	GCNGC	2 cut(s)	539, 707
BlsI	GCNGC	2 cut(s)	540, 708
BmcAI	AGTACT	2 cut(s)	86, 365
Bme1390I	CCNGG	3 cut(s)	272, 353, 393
Bme18I	GGWCC	1 cut(s)	819
BmeRI	GACNNNNNGTC	1 cut(s)	883
BmgT120I	GGNCC	1 cut(s)	819
BmiI	GGNNCC	2 cut(s)	754, 821
BmrFI	CCNGG	3 cut(s)	272, 353, 393
BmsI	GCATC	3 cut(s)	34, 408, 720
Bpu10I	CCTNAGC	1 cut(s)	309
BsaAI	YACGTR	1 cut(s)	223
BsaJI	CCNNGG	2 cut(s)	823, 960
BsaWI	WCCGGW	2 cut(s)	723, 952
Bse1I	ACTGG	1 cut(s)	220
Bse3DI	GCAATG	2 cut(s)	237, 667
BseAI	TCCGGA	1 cut(s)	952
BseBI	CCWGG	3 cut(s)	272, 353, 393
BseDI	CCNNGG	2 cut(s)	823, 960
BseGI	GGATG	3 cut(s)	361, 507, 735
BseMI	GCAATG	2 cut(s)	237, 667
BseMII	CTCAG	3 cut(s)	48, 282, 300
BseNI	ACTGG	1 cut(s)	220
BseXI	GCAGC	2 cut(s)	525, 718
BsgI	GTGCAG	1 cut(s)	657
BshFI	GGCC	2 cut(s)	270, 670
BsiSI	CCGG	2 cut(s)	724, 953
BslFI	GGGAC	2 cut(s)	805, 1057
BsmFI	GGGAC	2 cut(s)	805, 1057
BsnI	GGCC	2 cut(s)	270, 670
Bsp1286I	GDGCHC	1 cut(s)	652
Bsp13I	TCCGGA	1 cut(s)	952
Bsp143I	GATC	1 cut(s)	964
BspACI	CCGC	1 cut(s)	645
BspANI	GGCC	2 cut(s)	270, 670
BspCNI	CTCAG	3 cut(s)	49, 283, 301
BspEI	TCCGGA	1 cut(s)	952
BspLI	GGNNCC	2 cut(s)	754, 821
BspMAI	CTGCAG	1 cut(s)	708
BspPI	GGATC	1 cut(s)	972
BspQI	GCTCTTC	2 cut(s)	57, 657
BspTI	CTTAAG	1 cut(s)	242
BsrDI	GCAATG	2 cut(s)	237, 667
BsrI	ACTGG	1 cut(s)	220
BssECI	CCNNGG	2 cut(s)	823, 960
BssMI	GATC	1 cut(s)	964
BssNAI	GTATAC	1 cut(s)	605
BssT1I	CCWWGG	2 cut(s)	823, 960
Bst1107I	GTATAC	1 cut(s)	605
Bst2UI	CCWGG	3 cut(s)	272, 353, 393
Bst4CI	ACNGT	3 cut(s)	320, 472, 748
Bst6I	CTCTTC	3 cut(s)	57, 354, 657
BstAFI	CTTAAG	1 cut(s)	242
BstBAI	YACGTR	1 cut(s)	223
BstC8I	GCNNGC	2 cut(s)	672, 735
BstDEI	CTNAG	4 cut(s)	57, 291, 309, 641
BstF5I	GGATG	3 cut(s)	361, 507, 735
BstHHI	GCGC	1 cut(s)	423
BstKTI	GATC	1 cut(s)	967
BstMBI	GATC	1 cut(s)	964
BstMWI	GCNNNNNNNGC	2 cut(s)	706, 712
BstNI	CCWGG	3 cut(s)	272, 353, 393
BstNSI	RCATGY	1 cut(s)	284
BstSCI	CCNGG	3 cut(s)	270, 351, 391
BstSFI	CTRYAG	2 cut(s)	199, 704
BstV1I	GCAGC	2 cut(s)	525, 718
BstX2I	RGATCY	1 cut(s)	964
BstXI	CCANNNNNNTGG	1 cut(s)	1004
BstYI	RGATCY	1 cut(s)	964
BstZ17I	GTATAC	1 cut(s)	605
BsuI	GTATCC	1 cut(s)	421
BsuRI	GGCC	2 cut(s)	270, 670
BtsCI	GGATG	3 cut(s)	361, 507, 735
BtsIMutI	CAGTG	1 cut(s)	155
Cac8I	GCNNGC	2 cut(s)	672, 735
CfoI	GCGC	1 cut(s)	423
Cfr13I	GGNCC	1 cut(s)	819
Csp6I	GTAC	4 cut(s)	85, 364, 687, 838
CviAII	CATG	3 cut(s)	281, 559, 883
CviQI	GTAC	4 cut(s)	85, 364, 687, 838
DdeI	CTNAG	4 cut(s)	57, 291, 309, 641
DpnI	GATC	1 cut(s)	966
DpnII	GATC	1 cut(s)	964
DriI	GACNNNNNGTC	1 cut(s)	883
Eam1104I	CTCTTC	3 cut(s)	57, 354, 657
Eam1105I	GACNNNNNGTC	1 cut(s)	883
EarI	CTCTTC	3 cut(s)	57, 354, 657
Eco130I	CCWWGG	2 cut(s)	823, 960
Eco24I	GRGCYC	1 cut(s)	652
Eco32I	GATATC	1 cut(s)	857
Eco47I	GGWCC	1 cut(s)	819
Eco57I	CTGAAG	2 cut(s)	806, 954
EcoRII	CCWGG	3 cut(s)	270, 351, 391
EcoRV	GATATC	1 cut(s)	857
EcoT14I	CCWWGG	2 cut(s)	823, 960
EcoT38I	GRGCYC	1 cut(s)	652
ErhI	CCWWGG	2 cut(s)	823, 960
FaeI	CATG	3 cut(s)	284, 562, 886
FaqI	GGGAC	2 cut(s)	805, 1057
FatI	CATG	3 cut(s)	280, 558, 882
FauI	CCCGC	1 cut(s)	638
FblI	GTMKAC	1 cut(s)	604
Fnu4HI	GCNGC	2 cut(s)	539, 707
FokI	GGATG	3 cut(s)	368, 494, 742
FriOI	GRGCYC	1 cut(s)	652
Fsp4HI	GCNGC	2 cut(s)	539, 707
FspBI	CTAG	1 cut(s)	476
FspI	TGCGCA	1 cut(s)	422
GlaI	GCGC	1 cut(s)	422
GluI	GCNGC	2 cut(s)	539, 707
HaeIII	GGCC	2 cut(s)	270, 670
HapII	CCGG	2 cut(s)	724, 953
HhaI	GCGC	1 cut(s)	423
Hin1II	CATG	3 cut(s)	284, 562, 886
Hin6I	GCGC	1 cut(s)	421
HinP1I	GCGC	1 cut(s)	421
HincII	GTYRAC	3 cut(s)	316, 888, 994
HindII	GTYRAC	3 cut(s)	316, 888, 994
HindIII	AAGCTT	1 cut(s)	239
HinfI	GANTC	3 cut(s)	195, 298, 485
HpaII	CCGG	2 cut(s)	724, 953
Hpy166II	GTNNAC	6 cut(s)	316, 605, 689, 888, 977, 994
Hpy188I	TCNGA	7 cut(s)	58, 123, 253, 264, 571, 874, 924
Hpy188III	TCNNGA	3 cut(s)	611, 932, 953
Hpy8I	GTNNAC	6 cut(s)	316, 605, 689, 888, 977, 994
HpyAV	CCTTC	1 cut(s)	16
HpyCH4III	ACNGT	3 cut(s)	320, 472, 748
HpyCH4IV	ACGT	1 cut(s)	222
HpyCH4V	TGCA	6 cut(s)	13, 179, 230, 585, 674, 706
HpyF10VI	GCNNNNNNNGC	2 cut(s)	706, 712
HpyF3I	CTNAG	4 cut(s)	57, 291, 309, 641
HpySE526I	ACGT	1 cut(s)	222
Hsp92II	CATG	3 cut(s)	284, 562, 886
HspAI	GCGC	1 cut(s)	421
Kpn2I	TCCGGA	1 cut(s)	952
Kzo9I	GATC	1 cut(s)	964
LguI	GCTCTTC	2 cut(s)	57, 657
LmnI	GCTCC	2 cut(s)	25, 697
Lsp1109I	GCAGC	2 cut(s)	525, 718
LweI	GCATC	3 cut(s)	34, 408, 720
MaeI	CTAG	1 cut(s)	476
MaeII	ACGT	1 cut(s)	222
MaeIII	GTNAC	2 cut(s)	218, 554
MalI	GATC	1 cut(s)	966
MboI	GATC	1 cut(s)	964
MboII	GAAGA	8 cut(s)	74, 106, 247, 371, 380, 644, 799, 947
MflI	RGATCY	1 cut(s)	964
MhlI	GDGCHC	1 cut(s)	652
MluCI	AATT	9 cut(s)	322, 375, 502, 532, 563, 907, 926, 1034, 1048
MlyI	GAGTC	1 cut(s)	494
MmeI	TCCRAC	1 cut(s)	730
MnlI	CCTC	9 cut(s)	12, 52, 117, 181, 260, 288, 304, 720, 993
MroI	TCCGGA	1 cut(s)	952
MroXI	GAANNNNTTC	1 cut(s)	387
MseI	TTAA	3 cut(s)	243, 522, 906
MslI	CAYNNNNRTG	1 cut(s)	225
MspCI	CTTAAG	1 cut(s)	242
MspI	CCGG	2 cut(s)	724, 953
MspR9I	CCNGG	3 cut(s)	272, 353, 393
MvaI	CCWGG	3 cut(s)	272, 353, 393
MwoI	GCNNNNNNNGC	2 cut(s)	706, 712
NdeII	GATC	1 cut(s)	964
NlaIII	CATG	3 cut(s)	284, 562, 886
NlaIV	GGNNCC	2 cut(s)	754, 821
NmuCI	GTSAC	2 cut(s)	218, 554
NsbI	TGCGCA	1 cut(s)	422
NspI	RCATGY	1 cut(s)	284
PciI	ACATGT	1 cut(s)	280
PciSI	GCTCTTC	2 cut(s)	57, 657
PdmI	GAANNNNTTC	1 cut(s)	387
PfeI	GAWTC	2 cut(s)	195, 298
PfoI	TCCNGGA	1 cut(s)	351
PkrI	GCNGC	2 cut(s)	540, 708
PleI	GAGTC	1 cut(s)	493
PpsI	GAGTC	1 cut(s)	493
Ppu21I	YACGTR	1 cut(s)	223
PscI	ACATGT	1 cut(s)	280
Psp6I	CCWGG	3 cut(s)	270, 351, 391
PspGI	CCWGG	3 cut(s)	270, 351, 391
PspN4I	GGNNCC	2 cut(s)	754, 821
PspPI	GGNCC	1 cut(s)	819
PstI	CTGCAG	1 cut(s)	708
PsuI	RGATCY	1 cut(s)	964
RsaI	GTAC	4 cut(s)	86, 365, 688, 839
RsaNI	GTAC	4 cut(s)	85, 364, 687, 838
RseI	CAYNNNNRTG	1 cut(s)	225
SapI	GCTCTTC	2 cut(s)	57, 657
SaqAI	TTAA	3 cut(s)	243, 522, 906
SatI	GCNGC	2 cut(s)	539, 707
Sau3AI	GATC	1 cut(s)	964
Sau96I	GGNCC	1 cut(s)	819
ScaI	AGTACT	2 cut(s)	86, 365
SchI	GAGTC	1 cut(s)	494
ScrFI	CCNGG	3 cut(s)	272, 353, 393
SduI	GDGCHC	1 cut(s)	652
SetI	ASST	9 cut(s)	192, 225, 243, 276, 292, 315, 339, 451, 481
SfaNI	GCATC	3 cut(s)	34, 408, 720
SfcI	CTRYAG	2 cut(s)	199, 704
SinI	GGWCC	1 cut(s)	819
SmiMI	CAYNNNNRTG	1 cut(s)	225
SmlI	CTYRAG	1 cut(s)	242
SmoI	CTYRAG	1 cut(s)	242
Sse9I	AATT	9 cut(s)	322, 375, 502, 532, 563, 907, 926, 1034, 1048
SsiI	CCGC	1 cut(s)	645
SspMI	CTAG	1 cut(s)	476
StyD4I	CCNGG	3 cut(s)	270, 351, 391
StyI	CCWWGG	2 cut(s)	823, 960
TaaI	ACNGT	3 cut(s)	320, 472, 748
TaiI	ACGT	1 cut(s)	225
TasI	AATT	9 cut(s)	322, 375, 502, 532, 563, 907, 926, 1034, 1048
TatI	WGTACW	2 cut(s)	84, 363
TfiI	GAWTC	2 cut(s)	195, 298
Tru1I	TTAA	3 cut(s)	243, 522, 906
Tru9I	TTAA	3 cut(s)	243, 522, 906
TscAI	CASTG	1 cut(s)	162
TseFI	GTSAC	2 cut(s)	218, 554
TseI	GCWGC	2 cut(s)	538, 706
Tsp45I	GTSAC	2 cut(s)	218, 554
TspDTI	ATGAA	7 cut(s)	17, 248, 372, 459, 482, 575, 1038
TspRI	CASTG	1 cut(s)	162
Vha464I	CTTAAG	1 cut(s)	242
VpaK11BI	GGWCC	1 cut(s)	819
XapI	RAATTY	1 cut(s)	926
XceI	RCATGY	1 cut(s)	284
XmiI	GTMKAC	1 cut(s)	604
XmnI	GAANNNNTTC	1 cut(s)	387
XspI	CTAG	1 cut(s)	476
ZrmI	AGTACT	2 cut(s)	86, 365

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.