Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004270

Physical Location & Seq

Genomic Coordinates Forward (+)

21977526 .. 21984192

Gene Structure 6667 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1220500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGAGTTTGTAAGGTCATGGTTTAAAAGATTCAAGTCCAAGGAGCAAGTGAAGTCGTCAAAGAACAAGGATACTACAGGACATGGGAAAGAGGGTTCAAAAACCCCAACAACTGAAGAAGTGCCTTCAAATGTAACCAAGCAGAGGGTTGCAGCTGCAAAGCAGTATATAGAGGATCATTACAAGAAGCAGATGAAAAGTTTGCAGGAGAGGAAGGAGCGACGTAATATACTAGAAAAGAAGTTGGCTGATGCTGATGTCTCTGAGGAAGAGCAAAGCAACTTGCTAAAGTACTTGGAGAAGAAAGAAACAGAATATATGCGTATTCAGAGGCATAAAATGGGCGCTGATGATTTCGAGCCACTGACAATGATAGGAAAGGGTGCATTTGGAGAGGTTAGAATCTGTAGGGAGAAAGCAACTGGTCACGTATATGCAATGAAGAAGCTTAAGAAATCAGAGATGCTTCGGAGAGGCCAGGTTGAACATGTGAAAGCTGAGAGGAATCTACTTGCAGAGGTTGAGAGTAATTGTATCGTCAAGCTCTATTGTTCGTTCCAGGATGAAGAGTACTTGTATCTAATTATGGAATATCTTCCTGGTGGGGATATGATGACTTTATTGATGCGCAAGGATACACTGACAGAAGATGAGGCTAGATTTTATATTGGCGAAACCATCCTAGCTATTGAGTCCATTCATAAGCATAATTACATTCATAGAGATATTAAGCCTGACAATTTGCTGCTGGATAGAAATGGTCACATGAAATTATCAGATTTTGGATTATGTAAACCATTAGACTGTAGCATACTTCAAGAAAAGGATTTTTCTGTGGGAAAAAATCTTAGCGGGGCTCTTCAAAGTGATGGACGCCCTGCACCGCCAAAGCGTACACAACAGGAGCAACTGCAGCATTGGCAGAGAAACCGGAGGATGCTTGCTTATTCTACTGTTGGAACCCCAGACTACATTGCCCCCGAAGTTTTGCTGAAGAAAGGATATGGAATGGAATGTGATTGGTGGTCTCTTGGGGCTATCATGTATGAAATGCTTGTGGGATATCCACCCTTTTATTCAGATGAACCCATGTCAACTTGTAGAAAGATAGTTAACTGGAGAACGCATCTGAAATTTCCTGAAGAAGCTAAACTGTCTCCGGAAGCGAAAAATCTCATTAGTAAACTGTTATGTAATGTTGATCAGAGGCTTGGAACTAAAGGGGCAGATGAAATAAAGGCTCACTCTTGGTTTAAAGGCGTTGAATGGGACAAATTGTACCAAATGAAAGCTGCATTTATTCCCGAGGTCAATGATGAGTTGGATACCCAAAATTTTGAGAAGTTTGAGGAGGGAGACAACCAGATTGAAAGTTCAACGAGAGCTGGTCCATGGAGAAAGATGCTGTCTTCTAAAGATGTCAACTTCGTGGGTTACACATACAAGAACTTTGAAATTGTAAACGATGACCAATTGCCTGGGATTGCTGAATTGAAGAAGAGCACAAAACCTAAGCGGCCCTCCATCAAGTCTCTTTTTGACAATGAGTCTGCGACGGCGGCCAATCAACCTGTTCAAGGAAGCTTTATGAAACTCCTCCCTCCCCAACTAGAAGTCCCTGAGAAACAAACAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0010975 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0022008 GO:0023052 GO:0030154 GO:0030554 GO:0031344 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0045664 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050773 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051716 GO:0051960 GO:0060284 GO:0065007 GO:0071704 GO:0097159 GO:0097367 GO:0120035 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_C

Protein Analysis

546

Amino Acids

63.03

Weight (kDa)

8.34

Isoelectric Point (pI)

43.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	121 - 268	3.3e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	123 - 267	2.7e-22	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	315 - 418	1.4e-21	Protein kinase domain
Pkinase_C	PF00433	437 - 481	2.8e-07	Protein kinase C terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004270)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23310 AT4G14350 AT4G14350 AT4G14350 AT4G14350
fragaria_vesca	FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790
malus_domestica	MD13G1215300.v1.1 MD16G1220500.v1.1
prunus_persica	Prupe.1G040500_v2.0.a1 Prupe.1G040500_v2.0.a1 Prupe.1G040500_v2.0.a1
pyrus_communis	pycom13g18850 pycom16g18470
rosa_chinensis	RchiOBHm_Chr4g0393641
rosa_laevigata	RLG00000009739
rosa_multiflora	Rmu_sc0006302.1_g000002
rosa_roxburghii	Rroxscaffold_5G00338810
rosa_rugosa	Rorug03G0372000 Rorug03G0372100 Rorug03G0372200 Rorug03G0372300.1
rosa_samantha	Rh4AG048200 Rh4BG044700 Rh4CG051500 Rh4DG045500
rosa_wichuraiana	Rw4G003820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	629
AccIII	TCCGGA	1 cut(s)	1159
AciI	CCGC	4 cut(s)	852, 884, 1516, 1559
AclWI	GGATC	1 cut(s)	183
AcoI	YGGCCR	1 cut(s)	1560
AcsI	RAATTY	2 cut(s)	1133, 1333
AcuI	CTGAAG	3 cut(s)	135, 1013, 1161
AcyI	GRCGYC	1 cut(s)	874
AfaI	GTAC	4 cut(s)	293, 572, 895, 1280
AfiI	CCNNNNNNNGG	2 cut(s)	144, 1577
AflII	CTTAAG	1 cut(s)	449
AflIII	ACRYGT	1 cut(s)	487
AhdI	GACNNNNNGTC	1 cut(s)	1546
AjnI	CCWGG	4 cut(s)	477, 558, 598, 1477
AloI	GAACNNNNNNTCC	2 cut(s)	79, 111
AluBI	AGCT	9 cut(s)	155, 448, 497, 544, 686, 1148, 1292, 1385, 1584
AluI	AGCT	9 cut(s)	155, 448, 497, 544, 686, 1148, 1292, 1385, 1584
Alw21I	GWGCWC	1 cut(s)	1505
Alw26I	GTCTC	5 cut(s)	265, 1032, 1161, 1350, 1536
AlwI	GGATC	1 cut(s)	183
Ama87I	CYCGRG	1 cut(s)	1304
Aor13HI	TCCGGA	1 cut(s)	1159
AoxI	GGCC	3 cut(s)	475, 1517, 1560
ApeKI	GCWGC	5 cut(s)	152, 155, 745, 913, 1292
ApoI	RAATTY	2 cut(s)	1133, 1333
Asp700I	GAANNNNTTC	1 cut(s)	594
AspLEI	GCGC	2 cut(s)	347, 630
AspS9I	GGNCC	2 cut(s)	1388, 1518
AvaI	CYCGRG	1 cut(s)	1304
AvaII	GGWCC	1 cut(s)	1388
BanII	GRGCYC	1 cut(s)	859
BbsI	GAAGAC	1 cut(s)	1401
Bbv12I	GWGCWC	1 cut(s)	1505
BbvI	GCAGC	5 cut(s)	142, 164, 732, 925, 1279
BccI	CCATC	3 cut(s)	686, 863, 1532
BceAI	ACGGC	1 cut(s)	1572
BciT130I	CCWGG	4 cut(s)	479, 560, 600, 1479
BciVI	GTATCC	3 cut(s)	64, 628, 1318
BclI	TGATCA	1 cut(s)	1201
BcoDI	GTCTC	5 cut(s)	265, 1032, 1161, 1350, 1536
BfaI	CTAG	4 cut(s)	233, 657, 683, 1610
BfmI	CTRYAG	4 cut(s)	75, 406, 805, 911
BfoI	RGCGCY	1 cut(s)	348
BfrI	CTTAAG	1 cut(s)	449
BfuI	GTATCC	3 cut(s)	64, 628, 1318
BisI	GCNGC	7 cut(s)	153, 156, 746, 914, 1293, 1517, 1560
BlsI	GCNGC	7 cut(s)	154, 157, 747, 915, 1294, 1518, 1561
BmcAI	AGTACT	2 cut(s)	293, 572
Bme1390I	CCNGG	4 cut(s)	479, 560, 600, 1479
Bme18I	GGWCC	1 cut(s)	1388
BmeRI	GACNNNNNGTC	1 cut(s)	1546
BmeT110I	CYCGRG	1 cut(s)	1304
BmgT120I	GGNCC	2 cut(s)	1388, 1518
BmiI	GGNNCC	1 cut(s)	961
BmrFI	CCNGG	4 cut(s)	479, 560, 600, 1479
BmsI	GCATC	6 cut(s)	241, 453, 615, 927, 1135, 1392
BpiI	GAAGAC	1 cut(s)	1401
BpmI	CTGGAG	1 cut(s)	1138
Bpu10I	CCTNAGC	1 cut(s)	1512
BsaAI	YACGTR	1 cut(s)	430
BsaHI	GRCGYC	1 cut(s)	874
BsaI	GGTCTC	1 cut(s)	1032
BsaJI	CCNNGG	4 cut(s)	39, 1305, 1391, 1478
BsaWI	WCCGGW	2 cut(s)	930, 1159
Bsc4I	CCNNNNNNNGG	2 cut(s)	144, 1577
Bse1I	ACTGG	2 cut(s)	427, 1121
Bse3DI	GCAATG	2 cut(s)	444, 972
BseAI	TCCGGA	1 cut(s)	1159
BseBI	CCWGG	4 cut(s)	479, 560, 600, 1479
BseDI	CCNNGG	4 cut(s)	39, 1305, 1391, 1478
BseGI	GGATG	3 cut(s)	568, 678, 942
BseLI	CCNNNNNNNGG	2 cut(s)	144, 1577
BseMI	GCAATG	2 cut(s)	444, 972
BseMII	CTCAG	3 cut(s)	255, 489, 1611
BseNI	ACTGG	2 cut(s)	427, 1121
BseRI	GAGGAG	2 cut(s)	1364, 1586
BseXI	GCAGC	5 cut(s)	142, 164, 732, 925, 1279
BsgI	GTGCAG	1 cut(s)	864
BshFI	GGCC	3 cut(s)	477, 1519, 1562
BsiHKAI	GWGCWC	1 cut(s)	1505
BsiHKCI	CYCGRG	1 cut(s)	1304
BsiSI	CCGG	2 cut(s)	931, 1160
BslFI	GGGAC	2 cut(s)	1283, 1601
BslI	CCNNNNNNNGG	2 cut(s)	144, 1577
BsmAI	GTCTC	5 cut(s)	265, 1032, 1161, 1350, 1536
BsmFI	GGGAC	2 cut(s)	1283, 1601
BsnI	GGCC	3 cut(s)	477, 1519, 1562
Bso31I	GGTCTC	1 cut(s)	1032
BsoBI	CYCGRG	1 cut(s)	1304
Bsp1286I	GDGCHC	2 cut(s)	859, 1505
Bsp13I	TCCGGA	1 cut(s)	1159
Bsp143I	GATC	2 cut(s)	175, 1201
Bsp19I	CCATGG	1 cut(s)	1391
BspACI	CCGC	4 cut(s)	852, 884, 1516, 1559
BspANI	GGCC	3 cut(s)	477, 1519, 1562
BspCNI	CTCAG	3 cut(s)	256, 490, 1612
BspEI	TCCGGA	1 cut(s)	1159
BspLI	GGNNCC	1 cut(s)	961
BspMAI	CTGCAG	1 cut(s)	915
BspPI	GGATC	1 cut(s)	183
BspQI	GCTCTTC	3 cut(s)	264, 864, 1493
BspTI	CTTAAG	1 cut(s)	449
BspTNI	GGTCTC	1 cut(s)	1032
BsrDI	GCAATG	2 cut(s)	444, 972
BsrI	ACTGG	2 cut(s)	427, 1121
BssECI	CCNNGG	4 cut(s)	39, 1305, 1391, 1478
BssMI	GATC	2 cut(s)	175, 1201
BssNI	GRCGYC	1 cut(s)	874
BssT1I	CCWWGG	2 cut(s)	39, 1391
Bst2UI	CCWGG	4 cut(s)	479, 560, 600, 1479
Bst4CI	ACNGT	4 cut(s)	806, 955, 1155, 1188
Bst6I	CTCTTC	4 cut(s)	264, 561, 864, 1493
BstACI	GRCGYC	1 cut(s)	874
BstAFI	CTTAAG	1 cut(s)	449
BstBAI	YACGTR	1 cut(s)	430
BstC8I	GCNNGC	1 cut(s)	942
BstDEI	CTNAG	5 cut(s)	264, 498, 848, 1512, 1620
BstDSI	CCRYGG	1 cut(s)	1391
BstENI	CCTNNNNNAGG	1 cut(s)	1575
BstF5I	GGATG	3 cut(s)	568, 678, 942
BstH2I	RGCGCY	1 cut(s)	348
BstHHI	GCGC	2 cut(s)	347, 630
BstKTI	GATC	2 cut(s)	178, 1204
BstMAI	GTCTC	5 cut(s)	265, 1032, 1161, 1350, 1536
BstMBI	GATC	2 cut(s)	175, 1201
BstMWI	GCNNNNNNNGC	3 cut(s)	913, 919, 1559
BstNI	CCWGG	4 cut(s)	479, 560, 600, 1479
BstNSI	RCATGY	1 cut(s)	491
BstSCI	CCNGG	4 cut(s)	477, 558, 598, 1477
BstSFI	CTRYAG	4 cut(s)	75, 406, 805, 911
BstV1I	GCAGC	5 cut(s)	142, 164, 732, 925, 1279
BstV2I	GAAGAC	1 cut(s)	1401
BstXI	CCANNNNNNTGG	1 cut(s)	1478
BsuI	GTATCC	3 cut(s)	64, 628, 1318
BsuRI	GGCC	3 cut(s)	477, 1519, 1562
BtgI	CCRYGG	1 cut(s)	1391
BtsCI	GGATG	3 cut(s)	568, 678, 942
BtsIMutI	CAGTG	2 cut(s)	362, 638
Cac8I	GCNNGC	1 cut(s)	942
CfoI	GCGC	2 cut(s)	347, 630
Cfr13I	GGNCC	2 cut(s)	1388, 1518
CseI	GACGC	1 cut(s)	882
Csp6I	GTAC	4 cut(s)	292, 571, 894, 1279
CviAII	CATG	7 cut(s)	18, 83, 488, 766, 1042, 1090, 1392
CviQI	GTAC	4 cut(s)	292, 571, 894, 1279
DdeI	CTNAG	5 cut(s)	264, 498, 848, 1512, 1620
DpnI	GATC	2 cut(s)	177, 1203
DpnII	GATC	2 cut(s)	175, 1201
DraI	TTTAAA	2 cut(s)	25, 1255
DriI	GACNNNNNGTC	1 cut(s)	1546
EaeI	YGGCCR	1 cut(s)	1560
Eam1104I	CTCTTC	4 cut(s)	264, 561, 864, 1493
Eam1105I	GACNNNNNGTC	1 cut(s)	1546
EarI	CTCTTC	4 cut(s)	264, 561, 864, 1493
Eco130I	CCWWGG	2 cut(s)	39, 1391
Eco24I	GRGCYC	1 cut(s)	859
Eco31I	GGTCTC	1 cut(s)	1032
Eco32I	GATATC	1 cut(s)	1064
Eco47I	GGWCC	1 cut(s)	1388
Eco57I	CTGAAG	3 cut(s)	135, 1013, 1161
Eco88I	CYCGRG	1 cut(s)	1304
EcoNI	CCTNNNNNAGG	1 cut(s)	1575
EcoRII	CCWGG	4 cut(s)	477, 558, 598, 1477
EcoRV	GATATC	1 cut(s)	1064
EcoT14I	CCWWGG	2 cut(s)	39, 1391
EcoT38I	GRGCYC	1 cut(s)	859
ErhI	CCWWGG	2 cut(s)	39, 1391
FaeI	CATG	7 cut(s)	21, 86, 491, 769, 1045, 1093, 1395
FaqI	GGGAC	2 cut(s)	1283, 1601
FatI	CATG	7 cut(s)	17, 82, 487, 765, 1041, 1089, 1391
FauI	CCCGC	1 cut(s)	845
FbaI	TGATCA	1 cut(s)	1201
Fnu4HI	GCNGC	7 cut(s)	153, 156, 746, 914, 1293, 1517, 1560
FokI	GGATG	3 cut(s)	575, 665, 949
FriOI	GRGCYC	1 cut(s)	859
Fsp4HI	GCNGC	7 cut(s)	153, 156, 746, 914, 1293, 1517, 1560
FspBI	CTAG	4 cut(s)	233, 657, 683, 1610
FspI	TGCGCA	1 cut(s)	629
GlaI	GCGC	2 cut(s)	346, 629
GluI	GCNGC	7 cut(s)	153, 156, 746, 914, 1293, 1517, 1560
GsuI	CTGGAG	1 cut(s)	1138
HaeII	RGCGCY	1 cut(s)	348
HaeIII	GGCC	3 cut(s)	477, 1519, 1562
HapII	CCGG	2 cut(s)	931, 1160
HgaI	GACGC	1 cut(s)	882
HhaI	GCGC	2 cut(s)	347, 630
Hin1I	GRCGYC	1 cut(s)	874
Hin1II	CATG	7 cut(s)	21, 86, 491, 769, 1045, 1093, 1395
Hin6I	GCGC	2 cut(s)	345, 628
HinP1I	GCGC	2 cut(s)	345, 628
HincII	GTYRAC	3 cut(s)	1095, 1114, 1423
HindII	GTYRAC	3 cut(s)	1095, 1114, 1423
HindIII	AAGCTT	2 cut(s)	446, 1582
HinfI	GANTC	5 cut(s)	30, 402, 505, 692, 1547
HpaI	GTTAAC	1 cut(s)	1114
HpaII	CCGG	2 cut(s)	931, 1160
Hpy166II	GTNNAC	7 cut(s)	794, 896, 1095, 1114, 1184, 1423, 1462
Hpy188I	TCNGA	8 cut(s)	265, 330, 460, 471, 778, 1081, 1131, 1206
Hpy188III	TCNNGA	4 cut(s)	818, 1139, 1160, 1304
Hpy8I	GTNNAC	7 cut(s)	794, 896, 1095, 1114, 1184, 1423, 1462
Hpy99I	CGWCG	2 cut(s)	225, 1558
HpyAV	CCTTC	2 cut(s)	135, 208
HpyCH4III	ACNGT	4 cut(s)	806, 955, 1155, 1188
HpyCH4IV	ACGT	2 cut(s)	223, 429
HpyCH4V	TGCA	9 cut(s)	152, 158, 205, 386, 437, 515, 881, 913, 1295
HpyF10VI	GCNNNNNNNGC	3 cut(s)	913, 919, 1559
HpyF3I	CTNAG	5 cut(s)	264, 498, 848, 1512, 1620
HpySE526I	ACGT	2 cut(s)	223, 429
Hsp92I	GRCGYC	1 cut(s)	874
Hsp92II	CATG	7 cut(s)	21, 86, 491, 769, 1045, 1093, 1395
HspAI	GCGC	2 cut(s)	345, 628
Kpn2I	TCCGGA	1 cut(s)	1159
Ksp22I	TGATCA	1 cut(s)	1201
KspAI	GTTAAC	1 cut(s)	1114
Kzo9I	GATC	2 cut(s)	175, 1201
LguI	GCTCTTC	3 cut(s)	264, 864, 1493
LmnI	GCTCC	3 cut(s)	43, 217, 904
Lsp1109I	GCAGC	5 cut(s)	142, 164, 732, 925, 1279
LweI	GCATC	6 cut(s)	241, 453, 615, 927, 1135, 1392
MaeI	CTAG	4 cut(s)	233, 657, 683, 1610
MaeII	ACGT	2 cut(s)	223, 429
MaeIII	GTNAC	4 cut(s)	133, 425, 761, 1433
MalI	GATC	2 cut(s)	177, 1203
MboI	GATC	2 cut(s)	175, 1201
MfeI	CAATTG	1 cut(s)	1472
MhlI	GDGCHC	2 cut(s)	859, 1505
MlyI	GAGTC	2 cut(s)	701, 1556
MmeI	TCCRAC	2 cut(s)	937, 1302
MroI	TCCGGA	1 cut(s)	1159
MroXI	GAANNNNTTC	1 cut(s)	594
MseI	TTAA	5 cut(s)	24, 450, 729, 1113, 1254
MslI	CAYNNNNRTG	1 cut(s)	432
MspA1I	CMGCKG	1 cut(s)	155
MspCI	CTTAAG	1 cut(s)	449
MspI	CCGG	2 cut(s)	931, 1160
MspR9I	CCNGG	4 cut(s)	479, 560, 600, 1479
MunI	CAATTG	1 cut(s)	1472
MvaI	CCWGG	4 cut(s)	479, 560, 600, 1479
MwoI	GCNNNNNNNGC	3 cut(s)	913, 919, 1559
NcoI	CCATGG	1 cut(s)	1391
NdeII	GATC	2 cut(s)	175, 1201
NlaIII	CATG	7 cut(s)	21, 86, 491, 769, 1045, 1093, 1395
NlaIV	GGNNCC	1 cut(s)	961
NmuCI	GTSAC	2 cut(s)	425, 761
NsbI	TGCGCA	1 cut(s)	629
NspI	RCATGY	1 cut(s)	491
PciI	ACATGT	1 cut(s)	487
PciSI	GCTCTTC	3 cut(s)	264, 864, 1493
PdmI	GAANNNNTTC	1 cut(s)	594
PfeI	GAWTC	3 cut(s)	30, 402, 505
PfoI	TCCNGGA	1 cut(s)	558
PkrI	GCNGC	7 cut(s)	154, 157, 747, 915, 1294, 1518, 1561
PleI	GAGTC	2 cut(s)	700, 1555
PpsI	GAGTC	2 cut(s)	700, 1555
Ppu21I	YACGTR	1 cut(s)	430
PscI	ACATGT	1 cut(s)	487
Psp6I	CCWGG	4 cut(s)	477, 558, 598, 1477
PspGI	CCWGG	4 cut(s)	477, 558, 598, 1477
PspN4I	GGNNCC	1 cut(s)	961
PspPI	GGNCC	2 cut(s)	1388, 1518
PstI	CTGCAG	1 cut(s)	915
PvuII	CAGCTG	1 cut(s)	155
RsaI	GTAC	4 cut(s)	293, 572, 895, 1280
RsaNI	GTAC	4 cut(s)	292, 571, 894, 1279
RseI	CAYNNNNRTG	1 cut(s)	432
SapI	GCTCTTC	3 cut(s)	264, 864, 1493
SaqAI	TTAA	5 cut(s)	24, 450, 729, 1113, 1254
SatI	GCNGC	7 cut(s)	153, 156, 746, 914, 1293, 1517, 1560
Sau3AI	GATC	2 cut(s)	175, 1201
Sau96I	GGNCC	2 cut(s)	1388, 1518
ScaI	AGTACT	2 cut(s)	293, 572
SchI	GAGTC	2 cut(s)	701, 1556
ScrFI	CCNGG	4 cut(s)	479, 560, 600, 1479
SduI	GDGCHC	2 cut(s)	859, 1505
SfaNI	GCATC	6 cut(s)	241, 453, 615, 927, 1135, 1392
SfcI	CTRYAG	4 cut(s)	75, 406, 805, 911
SinI	GGWCC	1 cut(s)	1388
SmiMI	CAYNNNNRTG	1 cut(s)	432
SmlI	CTYRAG	1 cut(s)	449
SmoI	CTYRAG	1 cut(s)	449
SsiI	CCGC	4 cut(s)	852, 884, 1516, 1559
SspMI	CTAG	4 cut(s)	233, 657, 683, 1610
StyD4I	CCNGG	4 cut(s)	477, 558, 598, 1477
StyI	CCWWGG	2 cut(s)	39, 1391
TaaI	ACNGT	4 cut(s)	806, 955, 1155, 1188
TaiI	ACGT	2 cut(s)	226, 432
TaqI	TCGA	1 cut(s)	357
TatI	WGTACW	2 cut(s)	291, 570
TauI	GCSGC	2 cut(s)	1519, 1562
TfiI	GAWTC	3 cut(s)	30, 402, 505
Tru1I	TTAA	5 cut(s)	24, 450, 729, 1113, 1254
Tru9I	TTAA	5 cut(s)	24, 450, 729, 1113, 1254
TscAI	CASTG	2 cut(s)	369, 645
TseFI	GTSAC	2 cut(s)	425, 761
TseI	GCWGC	5 cut(s)	152, 155, 745, 913, 1292
Tsp45I	GTSAC	2 cut(s)	425, 761
TspRI	CASTG	2 cut(s)	369, 645
Vha464I	CTTAAG	1 cut(s)	449
VpaK11BI	GGWCC	1 cut(s)	1388
XagI	CCTNNNNNAGG	1 cut(s)	1575
XapI	RAATTY	2 cut(s)	1133, 1333
XceI	RCATGY	1 cut(s)	491
XmnI	GAANNNNTTC	1 cut(s)	594
XspI	CTAG	4 cut(s)	233, 657, 683, 1610
ZrmI	AGTACT	2 cut(s)	293, 572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.