Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0004270

Physical Location & Seq

Genomic Coordinates Forward (+)

8027883 .. 8034730

Gene Structure 6848 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG044700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1416 bp

ATGGAGTTTGTAAGGTCCTTGTTGAAAAAATTCAAGTCCAAAGAGAAGGTGAAGTCTTCAAAGAGCAGGGATACAAGAGGCAATGGGAAAGAGGCGTCAAAAGGACCAACAGCAACGGAAGAAGTACCTTCAAATGTAACCAAGCAGAAGGTTGCAGCTGCAAAGCAGTATATAGAGAATCATTACAAGAAGCAAATGAAAAGTTTGCAGGAGAGGAAGGAGCGACGTGATATACTAGAAAAGAAGTTGGCTGATGCTGAAGTCTCTGAGGAAGAGCAAAACAACTTGCTAAAGTATTTAGAGAAGAAGGAAACAGAATATATGCGTATTCAGAGGCATAAGATGGGTGCTGATGATTTTGAGCCACTGACAATGATAGGAAAGGGTGCATTTGGTGAGGTTAGAATCTGTAAGGAGAAAGCAACTGGTCAGGTCTATGCAATGAAGAAGCTTAAGAAATCAGAAATGCTTCGGAGAGGCCAGGTCGAACATGTGAAAGCTGAGAGGAATCTACTTGCAGAGGTTGACAGTAATTGTATCGTCAAGCTCTACTGCTCCTTCCAGGATGACGAGTACTTATACCTCATTATGGAATATCTCCCTGGTGGAGATATGATGACTCTACTGATGCGCAAAGATACACTGACAGAAGATGAGGCCAGGTTTTATGTTGGGGAAACAGTCCTAGCTATTGAATCCATTCATAAACATAATTACATTCATAGAGATATTAAGCCTGACAACTTGCTGCTTGATAGACATGGTCACATGAAATTATCAGATTTTGGATTGTGTAAGCCATTGGACTGCAGCATTCTCCAAGAAAAGGATTTTTCTATGGGGAAAAACCTTAGTGGGGCGCTTCAAAGTGATGGACGCCCTGTAGCACCAAAACGTACACAACAGGAGCAACTGCAGCATTGGCAGAGAAATCGGAGGATGCTTGCTTATTCTACAGTTGGAACCCCAGACTATATTGCCCCGGAAGTTTTGCTGAAGAAAGGATATGGAATGGAATGTGATTGGTGGTCTCTTGGGGCTATCATGTATGAAATGCTTGTGGGATATCCACCCTTTTATTCAGATGAACCCATGTCAACTTGTAGGAAGATAGTTAATTGGAGGACTCATTTGAAATTTCCTGAAGAAGCTAAACTCTCTCCAGAAGCAAAGGATCTCATTGGTAAACTCTTATGTAATGTAGATCAGAGGCTTGGAACTAAAGGGGCAGATGAAATTAAGGCTCACCACTGGTTTAAAGGCATTGAATGGGACAAATTGTATCAAATAAAGGCTGCATTTATCCCTGAGGTCAATGATGAATTGGATACTCAGAACTTTGAGAAGTTTGAAGAGACTGACAACCAAATTGAAACGTCAGCAAGAGCTGGTCCATGGAGAAAGGTTCGTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0010975 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0022008 GO:0023052 GO:0030154 GO:0030554 GO:0031344 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0045664 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050773 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051716 GO:0051960 GO:0060284 GO:0065007 GO:0071704 GO:0097159 GO:0097367 GO:0120035 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_C

Protein Analysis

471

Amino Acids

54.64

Weight (kDa)

8.61

Isoelectric Point (pI)

37.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	122 - 269	5.8e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	124 - 268	1.6e-22	Protein tyrosine and serine/threonine kinase
ABC1	PF03109	194 - 269	9.4e-06	ABC1 atypical kinase-like domain
Pkinase	PF00069	316 - 419	1.4e-21	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004270)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23310 AT4G14350 AT4G14350 AT4G14350 AT4G14350
fragaria_vesca	FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790 FvH4_4g03790
malus_domestica	MD13G1215300.v1.1 MD16G1220500.v1.1
prunus_persica	Prupe.1G040500_v2.0.a1 Prupe.1G040500_v2.0.a1 Prupe.1G040500_v2.0.a1
pyrus_communis	pycom13g18850 pycom16g18470
rosa_chinensis	RchiOBHm_Chr4g0393641
rosa_laevigata	RLG00000009739
rosa_multiflora	Rmu_sc0006302.1_g000002
rosa_roxburghii	Rroxscaffold_5G00338810
rosa_rugosa	Rorug03G0372000 Rorug03G0372100 Rorug03G0372200 Rorug03G0372300.1
rosa_samantha	Rh4AG048200 Rh4BG044700 Rh4CG051500 Rh4DG045500
rosa_wichuraiana	Rw4G003820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	632
AclWI	GGATC	1 cut(s)	1182
AcsI	RAATTY	2 cut(s)	29, 1136
AcuI	CTGAAG	3 cut(s)	279, 1016, 1164
AcyI	GRCGYC	2 cut(s)	95, 877
AfaI	GTAC	3 cut(s)	126, 575, 898
AfiI	CCNNNNNNNGG	3 cut(s)	589, 826, 856
AflII	CTTAAG	1 cut(s)	452
AflIII	ACRYGT	1 cut(s)	490
AjiI	CACGTC	1 cut(s)	227
AjnI	CCWGG	4 cut(s)	480, 561, 601, 659
AluBI	AGCT	7 cut(s)	158, 451, 500, 547, 689, 1151, 1388
AluI	AGCT	7 cut(s)	158, 451, 500, 547, 689, 1151, 1388
Alw26I	GTCTC	3 cut(s)	268, 1035, 1349
AlwI	GGATC	1 cut(s)	1182
AoxI	GGCC	2 cut(s)	478, 657
ApeKI	GCWGC	6 cut(s)	155, 158, 748, 810, 916, 1295
ApoI	RAATTY	2 cut(s)	29, 1136
Asp700I	GAANNNNTTC	3 cut(s)	29, 468, 699
AspLEI	GCGC	2 cut(s)	633, 862
AspS9I	GGNCC	3 cut(s)	15, 104, 1391
AsuC2I	CCSGG	1 cut(s)	983
AsuHPI	GGTGA	3 cut(s)	61, 407, 1238
AvaII	GGWCC	3 cut(s)	15, 104, 1391
AxyI	CCTNAGG	1 cut(s)	1308
BbsI	GAAGAC	1 cut(s)	48
BbvI	GCAGC	6 cut(s)	145, 167, 735, 822, 928, 1282
BccI	CCATC	2 cut(s)	337, 866
BcgI	CGANNNNNNTGC	2 cut(s)	914, 948
BciT130I	CCWGG	4 cut(s)	482, 563, 603, 661
BciVI	GTATCC	2 cut(s)	64, 1321
BcnI	CCSGG	1 cut(s)	983
BcoDI	GTCTC	3 cut(s)	268, 1035, 1349
BfaI	CTAG	2 cut(s)	236, 686
BfmI	CTRYAG	4 cut(s)	808, 882, 914, 954
BfoI	RGCGCY	1 cut(s)	863
BfrI	CTTAAG	1 cut(s)	452
BfuI	GTATCC	2 cut(s)	64, 1321
BisI	GCNGC	6 cut(s)	156, 159, 749, 811, 917, 1296
BlsI	GCNGC	6 cut(s)	157, 160, 750, 812, 918, 1297
BmcAI	AGTACT	1 cut(s)	575
Bme1390I	CCNGG	5 cut(s)	482, 563, 603, 661, 983
Bme18I	GGWCC	3 cut(s)	15, 104, 1391
BmgBI	CACGTC	1 cut(s)	227
BmgT120I	GGNCC	3 cut(s)	15, 104, 1391
BmiI	GGNNCC	1 cut(s)	964
BmrFI	CCNGG	5 cut(s)	482, 563, 603, 661, 983
BmsI	GCATC	3 cut(s)	244, 618, 930
BpiI	GAAGAC	1 cut(s)	48
BpmI	CTGGAG	1 cut(s)	1146
BpuMI	CCSGG	1 cut(s)	983
BsaHI	GRCGYC	2 cut(s)	95, 877
BsaI	GGTCTC	1 cut(s)	1035
BsaJI	CCNNGG	3 cut(s)	601, 981, 1394
Bsc4I	CCNNNNNNNGG	3 cut(s)	589, 826, 856
Bse1I	ACTGG	2 cut(s)	430, 1256
Bse21I	CCTNAGG	1 cut(s)	1308
Bse3DI	GCAATG	2 cut(s)	88, 447
BseBI	CCWGG	4 cut(s)	482, 563, 603, 661
BseDI	CCNNGG	3 cut(s)	601, 981, 1394
BseGI	GGATG	2 cut(s)	571, 945
BseLI	CCNNNNNNNGG	3 cut(s)	589, 826, 856
BseMI	GCAATG	2 cut(s)	88, 447
BseMII	CTCAG	4 cut(s)	258, 492, 1299, 1346
BseNI	ACTGG	2 cut(s)	430, 1256
BseXI	GCAGC	6 cut(s)	145, 167, 735, 822, 928, 1282
BshFI	GGCC	2 cut(s)	480, 659
BsiSI	CCGG	1 cut(s)	983
BslFI	GGGAC	1 cut(s)	1286
BslI	CCNNNNNNNGG	3 cut(s)	589, 826, 856
BsmAI	GTCTC	3 cut(s)	268, 1035, 1349
BsmFI	GGGAC	1 cut(s)	1286
BsmI	GAATGC	1 cut(s)	813
BsnI	GGCC	2 cut(s)	480, 659
Bso31I	GGTCTC	1 cut(s)	1035
Bsp143I	GATC	2 cut(s)	1174, 1204
Bsp19I	CCATGG	1 cut(s)	1394
BspANI	GGCC	2 cut(s)	480, 659
BspCNI	CTCAG	4 cut(s)	259, 493, 1300, 1345
BspLI	GGNNCC	1 cut(s)	964
BspMAI	CTGCAG	2 cut(s)	812, 918
BspPI	GGATC	1 cut(s)	1182
BspQI	GCTCTTC	1 cut(s)	267
BspTI	CTTAAG	1 cut(s)	452
BspTNI	GGTCTC	1 cut(s)	1035
BsrDI	GCAATG	2 cut(s)	88, 447
BsrI	ACTGG	2 cut(s)	430, 1256
BssECI	CCNNGG	3 cut(s)	601, 981, 1394
BssMI	GATC	2 cut(s)	1174, 1204
BssNI	GRCGYC	2 cut(s)	95, 877
BssT1I	CCWWGG	1 cut(s)	1394
Bst2UI	CCWGG	4 cut(s)	482, 563, 603, 661
Bst4CI	ACNGT	3 cut(s)	530, 682, 958
Bst6I	CTCTTC	2 cut(s)	267, 1347
BstACI	GRCGYC	2 cut(s)	95, 877
BstAFI	CTTAAG	1 cut(s)	452
BstC8I	GCNNGC	1 cut(s)	945
BstDEI	CTNAG	5 cut(s)	267, 501, 851, 1308, 1332
BstDSI	CCRYGG	1 cut(s)	1394
BstF5I	GGATG	2 cut(s)	571, 945
BstH2I	RGCGCY	1 cut(s)	863
BstHHI	GCGC	2 cut(s)	633, 862
BstKTI	GATC	2 cut(s)	1177, 1207
BstMAI	GTCTC	3 cut(s)	268, 1035, 1349
BstMBI	GATC	2 cut(s)	1174, 1204
BstMWI	GCNNNNNNNGC	2 cut(s)	916, 922
BstNI	CCWGG	4 cut(s)	482, 563, 603, 661
BstNSI	RCATGY	1 cut(s)	494
BstSCI	CCNGG	5 cut(s)	480, 561, 601, 659, 981
BstSFI	CTRYAG	4 cut(s)	808, 882, 914, 954
BstV1I	GCAGC	6 cut(s)	145, 167, 735, 822, 928, 1282
BstV2I	GAAGAC	1 cut(s)	48
BstX2I	RGATCY	1 cut(s)	1174
BstYI	RGATCY	1 cut(s)	1174
Bsu36I	CCTNAGG	1 cut(s)	1308
BsuI	GTATCC	2 cut(s)	64, 1321
BsuRI	GGCC	2 cut(s)	480, 659
BtgI	CCRYGG	1 cut(s)	1394
BtrI	CACGTC	1 cut(s)	227
BtsCI	GGATG	2 cut(s)	571, 945
BtsIMutI	CAGTG	3 cut(s)	365, 641, 1249
Cac8I	GCNNGC	1 cut(s)	945
CfoI	GCGC	2 cut(s)	633, 862
Cfr13I	GGNCC	3 cut(s)	15, 104, 1391
CseI	GACGC	2 cut(s)	84, 885
Csp6I	GTAC	3 cut(s)	125, 574, 897
CviAII	CATG	6 cut(s)	491, 761, 769, 1045, 1093, 1395
CviQI	GTAC	3 cut(s)	125, 574, 897
DdeI	CTNAG	5 cut(s)	267, 501, 851, 1308, 1332
DpnI	GATC	2 cut(s)	1176, 1206
DpnII	GATC	2 cut(s)	1174, 1204
DraI	TTTAAA	1 cut(s)	1258
Eam1104I	CTCTTC	2 cut(s)	267, 1347
EarI	CTCTTC	2 cut(s)	267, 1347
Eco130I	CCWWGG	1 cut(s)	1394
Eco31I	GGTCTC	1 cut(s)	1035
Eco32I	GATATC	1 cut(s)	1067
Eco47I	GGWCC	3 cut(s)	15, 104, 1391
Eco57I	CTGAAG	3 cut(s)	279, 1016, 1164
Eco81I	CCTNAGG	1 cut(s)	1308
EcoO109I	RGGNCCY	1 cut(s)	15
EcoRII	CCWGG	4 cut(s)	480, 561, 601, 659
EcoRV	GATATC	1 cut(s)	1067
EcoT14I	CCWWGG	1 cut(s)	1394
ErhI	CCWWGG	1 cut(s)	1394
FaeI	CATG	6 cut(s)	494, 764, 772, 1048, 1096, 1398
FaqI	GGGAC	1 cut(s)	1286
FatI	CATG	6 cut(s)	490, 760, 768, 1044, 1092, 1394
Fnu4HI	GCNGC	6 cut(s)	156, 159, 749, 811, 917, 1296
FokI	GGATG	2 cut(s)	578, 952
Fsp4HI	GCNGC	6 cut(s)	156, 159, 749, 811, 917, 1296
FspBI	CTAG	2 cut(s)	236, 686
FspI	TGCGCA	1 cut(s)	632
GlaI	GCGC	2 cut(s)	632, 861
GluI	GCNGC	6 cut(s)	156, 159, 749, 811, 917, 1296
GsuI	CTGGAG	1 cut(s)	1146
HaeII	RGCGCY	1 cut(s)	863
HaeIII	GGCC	2 cut(s)	480, 659
HapII	CCGG	1 cut(s)	983
HgaI	GACGC	2 cut(s)	84, 885
HhaI	GCGC	2 cut(s)	633, 862
Hin1I	GRCGYC	2 cut(s)	95, 877
Hin1II	CATG	6 cut(s)	494, 764, 772, 1048, 1096, 1398
Hin6I	GCGC	2 cut(s)	631, 860
HinP1I	GCGC	2 cut(s)	631, 860
HincII	GTYRAC	2 cut(s)	526, 1098
HindII	GTYRAC	2 cut(s)	526, 1098
HindIII	AAGCTT	1 cut(s)	449
HinfI	GANTC	6 cut(s)	178, 405, 508, 619, 695, 1126
HpaII	CCGG	1 cut(s)	983
HphI	GGTGA	3 cut(s)	61, 407, 1238
Hpy166II	GTNNAC	4 cut(s)	526, 899, 1098, 1187
Hpy188I	TCNGA	9 cut(s)	268, 333, 463, 474, 781, 936, 1084, 1209, 1335
Hpy188III	TCNNGA	2 cut(s)	1142, 1163
Hpy8I	GTNNAC	4 cut(s)	526, 899, 1098, 1187
Hpy99I	CGWCG	1 cut(s)	228
HpyAV	CCTTC	6 cut(s)	40, 138, 142, 211, 301, 568
HpyCH4III	ACNGT	3 cut(s)	530, 682, 958
HpyCH4IV	ACGT	3 cut(s)	226, 895, 1376
HpyCH4V	TGCA	9 cut(s)	155, 161, 208, 389, 440, 518, 810, 916, 1298
HpyF10VI	GCNNNNNNNGC	2 cut(s)	916, 922
HpyF3I	CTNAG	5 cut(s)	267, 501, 851, 1308, 1332
HpySE526I	ACGT	3 cut(s)	226, 895, 1376
Hsp92I	GRCGYC	2 cut(s)	95, 877
Hsp92II	CATG	6 cut(s)	494, 764, 772, 1048, 1096, 1398
HspAI	GCGC	2 cut(s)	631, 860
Kzo9I	GATC	2 cut(s)	1174, 1204
LguI	GCTCTTC	1 cut(s)	267
LmnI	GCTCC	3 cut(s)	220, 560, 907
Lsp1109I	GCAGC	6 cut(s)	145, 167, 735, 822, 928, 1282
LweI	GCATC	3 cut(s)	244, 618, 930
MaeI	CTAG	2 cut(s)	236, 686
MaeII	ACGT	3 cut(s)	226, 895, 1376
MaeIII	GTNAC	2 cut(s)	136, 764
MalI	GATC	2 cut(s)	1176, 1206
MboI	GATC	2 cut(s)	1174, 1204
MflI	RGATCY	1 cut(s)	1174
MlyI	GAGTC	2 cut(s)	613, 1120
MmeI	TCCRAC	1 cut(s)	940
MroXI	GAANNNNTTC	3 cut(s)	29, 468, 699
MseI	TTAA	5 cut(s)	453, 732, 1116, 1239, 1257
MspA1I	CMGCKG	1 cut(s)	158
MspCI	CTTAAG	1 cut(s)	452
MspI	CCGG	1 cut(s)	983
MspR9I	CCNGG	5 cut(s)	482, 563, 603, 661, 983
Mva1269I	GAATGC	1 cut(s)	813
MvaI	CCWGG	4 cut(s)	482, 563, 603, 661
MwoI	GCNNNNNNNGC	2 cut(s)	916, 922
NciI	CCSGG	1 cut(s)	983
NcoI	CCATGG	1 cut(s)	1394
NdeII	GATC	2 cut(s)	1174, 1204
NlaIII	CATG	6 cut(s)	494, 764, 772, 1048, 1096, 1398
NlaIV	GGNNCC	1 cut(s)	964
NmuCI	GTSAC	1 cut(s)	764
NsbI	TGCGCA	1 cut(s)	632
NspI	RCATGY	1 cut(s)	494
PciI	ACATGT	1 cut(s)	490
PciSI	GCTCTTC	1 cut(s)	267
PctI	GAATGC	1 cut(s)	813
PdmI	GAANNNNTTC	3 cut(s)	29, 468, 699
PfeI	GAWTC	4 cut(s)	178, 405, 508, 695
PflFI	GACNNNGTC	1 cut(s)	762
PfoI	TCCNGGA	1 cut(s)	561
PkrI	GCNGC	6 cut(s)	157, 160, 750, 812, 918, 1297
PleI	GAGTC	2 cut(s)	613, 1120
PpsI	GAGTC	2 cut(s)	613, 1120
PpuMI	RGGWCCY	1 cut(s)	15
PscI	ACATGT	1 cut(s)	490
Psp5II	RGGWCCY	1 cut(s)	15
Psp6I	CCWGG	4 cut(s)	480, 561, 601, 659
PspGI	CCWGG	4 cut(s)	480, 561, 601, 659
PspN4I	GGNNCC	1 cut(s)	964
PspPI	GGNCC	3 cut(s)	15, 104, 1391
PspPPI	RGGWCCY	1 cut(s)	15
PstI	CTGCAG	2 cut(s)	812, 918
PsuI	RGATCY	1 cut(s)	1174
PsyI	GACNNNGTC	1 cut(s)	762
PvuII	CAGCTG	1 cut(s)	158
RsaI	GTAC	3 cut(s)	126, 575, 898
RsaNI	GTAC	3 cut(s)	125, 574, 897
SapI	GCTCTTC	1 cut(s)	267
SaqAI	TTAA	5 cut(s)	453, 732, 1116, 1239, 1257
SatI	GCNGC	6 cut(s)	156, 159, 749, 811, 917, 1296
Sau3AI	GATC	2 cut(s)	1174, 1204
Sau96I	GGNCC	3 cut(s)	15, 104, 1391
ScaI	AGTACT	1 cut(s)	575
SchI	GAGTC	2 cut(s)	613, 1120
ScrFI	CCNGG	5 cut(s)	482, 563, 603, 661, 983
SfaNI	GCATC	3 cut(s)	244, 618, 930
SfcI	CTRYAG	4 cut(s)	808, 882, 914, 954
SinI	GGWCC	3 cut(s)	15, 104, 1391
SmlI	CTYRAG	1 cut(s)	452
SmoI	CTYRAG	1 cut(s)	452
SspMI	CTAG	2 cut(s)	236, 686
StyD4I	CCNGG	5 cut(s)	480, 561, 601, 659, 981
StyI	CCWWGG	1 cut(s)	1394
TaaI	ACNGT	3 cut(s)	530, 682, 958
TaiI	ACGT	3 cut(s)	229, 898, 1379
TaqI	TCGA	1 cut(s)	486
TatI	WGTACW	1 cut(s)	573
TfiI	GAWTC	4 cut(s)	178, 405, 508, 695
Tru1I	TTAA	5 cut(s)	453, 732, 1116, 1239, 1257
Tru9I	TTAA	5 cut(s)	453, 732, 1116, 1239, 1257
TscAI	CASTG	3 cut(s)	372, 648, 1256
TseFI	GTSAC	1 cut(s)	764
TseI	GCWGC	6 cut(s)	155, 158, 748, 810, 916, 1295
Tsp45I	GTSAC	1 cut(s)	764
TspDTI	ATGAA	9 cut(s)	212, 458, 692, 710, 785, 1065, 1101, 1248, 1335
TspGWI	ACGGA	1 cut(s)	131
TspRI	CASTG	3 cut(s)	372, 648, 1256
Tth111I	GACNNNGTC	1 cut(s)	762
Vha464I	CTTAAG	1 cut(s)	452
VpaK11BI	GGWCC	3 cut(s)	15, 104, 1391
XapI	RAATTY	2 cut(s)	29, 1136
XceI	RCATGY	1 cut(s)	494
XmnI	GAANNNNTTC	3 cut(s)	29, 468, 699
XspI	CTAG	2 cut(s)	236, 686
ZrmI	AGTACT	1 cut(s)	575

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.