Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004074

Physical Location & Seq

Genomic Coordinates Forward (+)

22060403 .. 22066195

Gene Structure 5793 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1225700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGGAAGTTTGTGGGATAATTTTTTAAGTTGGCTGTGGAATGATTTCGTTTGGTTCGAACGAAACTTCCCATTAGATAACGTTCTCAACGCAAAAGGAAGAGCGGACTCTCCATTCATGGAGGTCCTCGCTAGCTCTGCAACTGCATCTTCATCCCCTCATTTCCTACTCTCCAGCTTCTACAAGAACCACCGTAGCACCGTCTCCATGGCCGCCACTCCTCCGCCTAAGGTGCTGCAATGGAAGTATCTTCCAGTAAGGATTGTAGAAGCTCGAGCAAAGTCTCCTTTAATGTGTATAATGACTACGTCGCTTGAGAGGCCGATAAATTATGGTAATACTGATGGGGACCTTAAGCACAGTTTCTTGATTCCACTGGTGTTGAGGCTCAGTCGGAGTTTTGTTTGTCAGGGTGGTTTGTTTTCAGCAAATTATAGTAAGGTCAGGAAAAAACTTCGACTGAAGAAATATGCTGGCAGTCTCCATGAAGTGCTAAAATGTTCAGAAAAAATTAGACGGCATGTTTTTGTTTTCATTGCTGGACTGATGGTTGTCATGTCAGCTTCTCTTTCTGTTAGCATTACTCCATCCTGGGCTCTTACAGAAGAGAATCGTATCTTCTTAGAGGCATGGAAGATGATTGACCGTGCATATGTTGACAAAAGTTTTAATGGACAAAGTTGGTTTCGGTACAGAGAAAATGCATTGCGCAATGAACCAATGAACACAAGAGAAGAGACATATATGGCAATTAAGAAGATGCTTGCCACATTGGAGGATCCTTTCACTCGGTTTCTGGAGCCTGAAAAGTTCAATAGTCTACGGTCTGGAACTCAAGGTGCTCTTACAGGCGTAGGGCTGTCAATTGGCTACCCTACAAAACTTGATGGATCAGCTGCTGGTCTTGTTGTTATTTCAGCTTCCCCAGGAGGTCCAGCAAACAGAGCCGGCATCTTGTCTGGGGATGTTATTCTGGCTATTGATGATACAAGTACAGAAACAATGGGCATATATGATGCCGCAGAGCGACTACAGGGACCGGACGGAAGTACAGTTAAACTAACAGTTCGTAGTGGACCTGAAATAAAGCACCTGGATTTAATGCGGGAGAAAGTTTCACTGAATCCTGTAAAGTCAAGACTATGTGCGGTCCCTGCGTCCGGAAAGGATTCCCCTAGAATTGGCTATATCAAACTATCAACGTTCAATCAAAATGCATCTGGTGCTGTCAAGGAAGCAATTAATACCTTAAGGAGTAATAATGTTAATGCCTTTGTGTTGGATCTTCGAGACAATAGTGGTGGTCTTTTCCCAGAAGGAATTGAGATTGCTAAGATTTGGTTGGACAAAGGTGTGATTGTATATATTTGTGATGGTCGTGGTGTTCGAGATATATATGACACCGATGGAAGCCAAGCGGTGGCAACTTCAGAACCCCTGGTTGTGCTGGTGAACAAGGGAACTGCTAGCGCAAGTGAAATCTTAGCTGGGGCATTGAAAGACAATAAACGTGCTGTGTTGTTTGGAGAACCCACATTTGGGAAGGGCAAGATTCAGTCAGTTTTTGAGCTGTCTGATGGCTCTGGCTTGGTTGTTACAGTCGCTCGTTATGAGACACCTGCTCACACAGACATTGATAAGGTAGGTGTGATTCCAGACCATCCCCTGCCAACTTCATTCCCCAAAGATGAGGAGGCCTTTTGTAACTGCCTCCAGGACCCTGCATCTGCTTGCAACAAGGTCGAGCTATTTGCAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009579 GO:0016787 GO:0019538 GO:0031977 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044436 GO:0044444 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ PDZ_2 Peptidase_S41

Protein Analysis

587

Amino Acids

64.28

Weight (kDa)

8.7

Isoelectric Point (pI)

40.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PDZ	PF00595	284 - 356	2.8e-09	PDZ domain
PDZ_2	PF13180	300 - 364	3.8e-06	PDZ domain
PDZ_6	PF17820	303 - 357	4.1e-12	PDZ domain
Peptidase_S41	PF03572	393 - 554	2.1e-53	Peptidase family S41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17740 AT4G17740
fragaria_vesca	FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180
malus_domestica	MD13G1225700.v1.1 MD16G1230600.v1.1
prunus_persica	Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1
pyrus_communis	pycom13g19890 pycom16g19260
rosa_chinensis	RchiOBHm_Chr4g0396091
rosa_laevigata	RLG00000009573
rosa_multiflora	Rmu_sc0006325.1_g000018 Rmu_sc0007010.1_g000014 Rmu_sc0009634.1_g000011
rosa_roxburghii	Rroxscaffold_5G00340890
rosa_rugosa	Rorug03G0352600 Rorug03G0352600
rosa_samantha	Rh4BG064100 Rh4CG070700 Rh4DG061100
rosa_wichuraiana	Rw4G005320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1627
Acc16I	TGCGCA	1 cut(s)	710
Acc36I	ACCTGC	1 cut(s)	1627
AccB7I	CCANNNNNTGG	1 cut(s)	1420
AccBSI	CCGCTC	1 cut(s)	104
AccI	GTMKAC	1 cut(s)	820
AccIII	TCCGGA	1 cut(s)	1160
AciI	CCGC	7 cut(s)	104, 213, 224, 1020, 1105, 1148, 1418
AclI	AACGTT	2 cut(s)	81, 1202
AclWI	GGATC	4 cut(s)	773, 786, 898, 1290
AcoI	YGGCCR	1 cut(s)	210
AcuI	CTGAAG	2 cut(s)	482, 1413
AfaI	GTAC	3 cut(s)	692, 994, 1051
AfiI	CCNNNNNNNGG	5 cut(s)	1160, 1181, 1420, 1538, 1539
AflII	CTTAAG	2 cut(s)	353, 1249
AgsI	TTSAA	3 cut(s)	814, 1207, 1498
AjnI	CCWGG	5 cut(s)	590, 925, 1092, 1437, 1713
AjuI	GAANNNNNNNTTGG	2 cut(s)	1521, 1553
AloI	GAACNNNNNNTCC	2 cut(s)	51, 83
AluBI	AGCT	9 cut(s)	135, 177, 272, 563, 896, 920, 1487, 1570, 1747
AluI	AGCT	9 cut(s)	135, 177, 272, 563, 896, 920, 1487, 1570, 1747
Alw21I	GWGCWC	1 cut(s)	844
Alw26I	GTCTC	6 cut(s)	208, 288, 485, 731, 1284, 1607
AlwI	GGATC	4 cut(s)	773, 786, 898, 1290
AlwNI	CAGNNNCTG	1 cut(s)	899
Ama87I	CYCGRG	1 cut(s)	273
Aor13HI	TCCGGA	1 cut(s)	1160
AoxI	GGCC	3 cut(s)	210, 320, 1695
ApeKI	GCWGC	2 cut(s)	235, 896
AseI	ATTAAT	1 cut(s)	1242
Asp700I	GAANNNNTTC	2 cut(s)	44, 1168
AspLEI	GCGC	2 cut(s)	711, 1472
AspS9I	GGNCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
AsuHPI	GGTGA	1 cut(s)	1462
AsuII	TTCGAA	1 cut(s)	57
AsuNHI	GCTAGC	2 cut(s)	131, 1466
AvaI	CYCGRG	1 cut(s)	273
AvaII	GGWCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
AxyI	CCTNAGG	1 cut(s)	228
BamHI	GGATCC	1 cut(s)	778
BanII	GRGCYC	1 cut(s)	598
Bbv12I	GWGCWC	1 cut(s)	844
BbvI	GCAGC	2 cut(s)	222, 883
BccI	CCATC	8 cut(s)	338, 541, 595, 881, 1367, 1400, 1571, 1668
BceAI	ACGGC	1 cut(s)	533
BcgI	CGANNNNNNTGC	3 cut(s)	1723, 1733, 1757
BciT130I	CCWGG	5 cut(s)	592, 927, 1094, 1439, 1715
BcoDI	GTCTC	6 cut(s)	208, 288, 485, 731, 1284, 1607
BfaI	CTAG	3 cut(s)	132, 1176, 1467
BfmI	CTRYAG	1 cut(s)	1031
BfrI	CTTAAG	2 cut(s)	353, 1249
BfuAI	ACCTGC	1 cut(s)	1627
BisI	GCNGC	4 cut(s)	213, 236, 897, 1020
BlsI	GCNGC	4 cut(s)	214, 237, 898, 1021
Bme1390I	CCNGG	5 cut(s)	592, 927, 1094, 1439, 1715
Bme18I	GGWCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
BmeT110I	CYCGRG	1 cut(s)	273
BmgT120I	GGNCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
BmiI	GGNNCC	6 cut(s)	350, 780, 801, 1038, 1152, 1719
BmrFI	CCNGG	5 cut(s)	592, 927, 1094, 1439, 1715
BmsI	GCATC	6 cut(s)	155, 750, 960, 1006, 1226, 1733
BmtI	GCTAGC	2 cut(s)	135, 1470
BpmI	CTGGAG	3 cut(s)	157, 818, 1697
Bpu14I	TTCGAA	1 cut(s)	57
BpuEI	CTTGAG	2 cut(s)	335, 819
BsaJI	CCNNGG	4 cut(s)	207, 591, 925, 1437
BsaWI	WCCGGW	2 cut(s)	1039, 1160
Bsc4I	CCNNNNNNNGG	5 cut(s)	1160, 1181, 1420, 1538, 1539
Bse118I	RCCGGY	1 cut(s)	947
Bse1I	ACTGG	2 cut(s)	254, 381
Bse21I	CCTNAGG	1 cut(s)	228
Bse3DI	GCAATG	4 cut(s)	245, 534, 704, 718
BseAI	TCCGGA	1 cut(s)	1160
BseBI	CCWGG	5 cut(s)	592, 927, 1094, 1439, 1715
BseDI	CCNNGG	4 cut(s)	207, 591, 925, 1437
BseGI	GGATG	4 cut(s)	152, 587, 970, 1660
BseLI	CCNNNNNNNGG	5 cut(s)	1160, 1181, 1420, 1538, 1539
BseMI	GCAATG	4 cut(s)	245, 534, 704, 718
BseMII	CTCAG	1 cut(s)	403
BseNI	ACTGG	2 cut(s)	254, 381
BseRI	GAGGAG	2 cut(s)	210, 1706
BseXI	GCAGC	2 cut(s)	222, 883
BseYI	CCCAGC	1 cut(s)	1487
BshFI	GGCC	3 cut(s)	212, 322, 1697
BsiHKAI	GWGCWC	1 cut(s)	844
BsiHKCI	CYCGRG	1 cut(s)	273
BsiSI	CCGG	3 cut(s)	948, 1040, 1161
BslFI	GGGAC	3 cut(s)	362, 1050, 1136
BslI	CCNNNNNNNGG	5 cut(s)	1160, 1181, 1420, 1538, 1539
BsmAI	GTCTC	6 cut(s)	208, 288, 485, 731, 1284, 1607
BsmBI	CGTCTC	1 cut(s)	208
BsmFI	GGGAC	3 cut(s)	362, 1050, 1136
BsnI	GGCC	3 cut(s)	212, 322, 1697
BsoBI	CYCGRG	1 cut(s)	273
Bsp119I	TTCGAA	1 cut(s)	57
Bsp1286I	GDGCHC	2 cut(s)	598, 844
Bsp13I	TCCGGA	1 cut(s)	1160
Bsp143I	GATC	3 cut(s)	778, 890, 1282
Bsp19I	CCATGG	1 cut(s)	207
BspACI	CCGC	7 cut(s)	104, 213, 224, 1020, 1105, 1148, 1418
BspANI	GGCC	3 cut(s)	212, 322, 1697
BspCNI	CTCAG	1 cut(s)	402
BspEI	TCCGGA	1 cut(s)	1160
BspLI	GGNNCC	6 cut(s)	350, 780, 801, 1038, 1152, 1719
BspMI	ACCTGC	1 cut(s)	1627
BspOI	GCTAGC	2 cut(s)	135, 1470
BspPI	GGATC	4 cut(s)	773, 786, 898, 1290
BspQI	GCTCTTC	1 cut(s)	94
BspT104I	TTCGAA	1 cut(s)	57
BspTI	CTTAAG	2 cut(s)	353, 1249
BsrBI	CCGCTC	1 cut(s)	104
BsrDI	GCAATG	4 cut(s)	245, 534, 704, 718
BsrFI	RCCGGY	1 cut(s)	947
BsrI	ACTGG	2 cut(s)	254, 381
BssAI	RCCGGY	1 cut(s)	947
BssECI	CCNNGG	4 cut(s)	207, 591, 925, 1437
BssMI	GATC	3 cut(s)	778, 890, 1282
BssT1I	CCWWGG	1 cut(s)	207
Bst2UI	CCWGG	5 cut(s)	592, 927, 1094, 1439, 1715
Bst4CI	ACNGT	8 cut(s)	194, 202, 362, 647, 825, 1054, 1066, 1600
Bst6I	CTCTTC	3 cut(s)	94, 600, 729
BstAFI	CTTAAG	2 cut(s)	353, 1249
BstAPI	GCANNNNNTGC	1 cut(s)	1223
BstBI	TTCGAA	1 cut(s)	57
BstC8I	GCNNGC	6 cut(s)	133, 475, 765, 949, 1468, 1732
BstDEI	CTNAG	5 cut(s)	228, 389, 622, 1332, 1483
BstDSI	CCRYGG	1 cut(s)	207
BstF5I	GGATG	4 cut(s)	152, 587, 970, 1660
BstHHI	GCGC	2 cut(s)	711, 1472
BstKTI	GATC	3 cut(s)	781, 893, 1285
BstMAI	GTCTC	6 cut(s)	208, 288, 485, 731, 1284, 1607
BstMBI	GATC	3 cut(s)	778, 890, 1282
BstMWI	GCNNNNNNNGC	6 cut(s)	137, 232, 319, 944, 1154, 1223
BstNI	CCWGG	5 cut(s)	592, 927, 1094, 1439, 1715
BstNSI	RCATGY	1 cut(s)	524
BstSCI	CCNGG	5 cut(s)	590, 925, 1092, 1437, 1713
BstSFI	CTRYAG	1 cut(s)	1031
BstV1I	GCAGC	2 cut(s)	222, 883
BstX2I	RGATCY	2 cut(s)	778, 1282
BstYI	RGATCY	2 cut(s)	778, 1282
Bsu36I	CCTNAGG	1 cut(s)	228
BsuRI	GGCC	3 cut(s)	212, 322, 1697
BtgI	CCRYGG	1 cut(s)	207
BtsCI	GGATG	4 cut(s)	152, 587, 970, 1660
BtsIMutI	CAGTG	2 cut(s)	374, 1118
BveI	ACCTGC	1 cut(s)	1627
Cac8I	GCNNGC	6 cut(s)	133, 475, 765, 949, 1468, 1732
CaiI	CAGNNNCTG	1 cut(s)	899
CfoI	GCGC	2 cut(s)	711, 1472
Cfr10I	RCCGGY	1 cut(s)	947
Cfr13I	GGNCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
CseI	GACGC	1 cut(s)	1146
Csp6I	GTAC	3 cut(s)	691, 993, 1050
CspCI	CAANNNNNGTGG	4 cut(s)	363, 398, 1282, 1317
CviAII	CATG	7 cut(s)	118, 208, 485, 521, 556, 630, 1758
CviQI	GTAC	3 cut(s)	691, 993, 1050
DdeI	CTNAG	5 cut(s)	228, 389, 622, 1332, 1483
DpnI	GATC	3 cut(s)	780, 892, 1284
DpnII	GATC	3 cut(s)	778, 890, 1282
EaeI	YGGCCR	1 cut(s)	210
Eam1104I	CTCTTC	3 cut(s)	94, 600, 729
EarI	CTCTTC	3 cut(s)	94, 600, 729
EciI	GGCGGA	1 cut(s)	213
Eco130I	CCWWGG	1 cut(s)	207
Eco147I	AGGCCT	1 cut(s)	1697
Eco24I	GRGCYC	1 cut(s)	598
Eco47I	GGWCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
Eco57I	CTGAAG	2 cut(s)	482, 1413
Eco81I	CCTNAGG	1 cut(s)	228
Eco88I	CYCGRG	1 cut(s)	273
EcoO109I	RGGNCCY	3 cut(s)	124, 349, 1717
EcoRII	CCWGG	5 cut(s)	590, 925, 1092, 1437, 1713
EcoT14I	CCWWGG	1 cut(s)	207
EcoT22I	ATGCAT	2 cut(s)	706, 1219
EcoT38I	GRGCYC	1 cut(s)	598
ErhI	CCWWGG	1 cut(s)	207
Esp3I	CGTCTC	1 cut(s)	208
FaeI	CATG	7 cut(s)	121, 211, 488, 524, 559, 633, 1761
FalI	AAGNNNNNCTT	2 cut(s)	828, 860
FaqI	GGGAC	3 cut(s)	362, 1050, 1136
FatI	CATG	7 cut(s)	117, 207, 484, 520, 555, 629, 1757
FauI	CCCGC	1 cut(s)	1098
FauNDI	CATATG	1 cut(s)	652
FblI	GTMKAC	1 cut(s)	820
Fnu4HI	GCNGC	4 cut(s)	213, 236, 897, 1020
FokI	GGATG	4 cut(s)	139, 574, 977, 1647
FriOI	GRGCYC	1 cut(s)	598
Fsp4HI	GCNGC	4 cut(s)	213, 236, 897, 1020
FspBI	CTAG	3 cut(s)	132, 1176, 1467
FspI	TGCGCA	1 cut(s)	710
GlaI	GCGC	2 cut(s)	710, 1471
GluI	GCNGC	4 cut(s)	213, 236, 897, 1020
GsaI	CCCAGC	1 cut(s)	1491
GsuI	CTGGAG	3 cut(s)	157, 818, 1697
HaeIII	GGCC	3 cut(s)	212, 322, 1697
HapII	CCGG	3 cut(s)	948, 1040, 1161
HgaI	GACGC	1 cut(s)	1146
HhaI	GCGC	2 cut(s)	711, 1472
Hin1II	CATG	7 cut(s)	121, 211, 488, 524, 559, 633, 1761
Hin6I	GCGC	2 cut(s)	709, 1470
HinP1I	GCGC	2 cut(s)	709, 1470
HincII	GTYRAC	1 cut(s)	658
HindII	GTYRAC	1 cut(s)	658
HinfI	GANTC	7 cut(s)	107, 370, 610, 1123, 1169, 1552, 1651
HpaII	CCGG	3 cut(s)	948, 1040, 1161
HphI	GGTGA	1 cut(s)	1462
Hpy166II	GTNNAC	4 cut(s)	658, 821, 1076, 1453
Hpy188I	TCNGA	4 cut(s)	396, 505, 1432, 1576
Hpy188III	TCNNGA	9 cut(s)	367, 445, 797, 828, 1137, 1161, 1289, 1388, 1655
Hpy8I	GTNNAC	4 cut(s)	658, 821, 1076, 1453
Hpy99I	CGWCG	1 cut(s)	313
HpyAV	CCTTC	2 cut(s)	1310, 1537
HpyCH4III	ACNGT	8 cut(s)	194, 202, 362, 647, 825, 1054, 1066, 1600
HpyCH4IV	ACGT	4 cut(s)	81, 308, 1202, 1510
HpyCH4V	TGCA	9 cut(s)	140, 146, 238, 650, 704, 1217, 1724, 1734, 1754
HpyF10VI	GCNNNNNNNGC	6 cut(s)	137, 232, 319, 944, 1154, 1223
HpyF3I	CTNAG	5 cut(s)	228, 389, 622, 1332, 1483
HpySE526I	ACGT	4 cut(s)	81, 308, 1202, 1510
Hsp92II	CATG	7 cut(s)	121, 211, 488, 524, 559, 633, 1761
HspAI	GCGC	2 cut(s)	709, 1470
Kpn2I	TCCGGA	1 cut(s)	1160
KroI	GCCGGC	1 cut(s)	947
KroNI	GCCGGC	1 cut(s)	949
Kzo9I	GATC	3 cut(s)	778, 890, 1282
LguI	GCTCTTC	1 cut(s)	94
LmnI	GCTCC	1 cut(s)	799
Lsp1109I	GCAGC	2 cut(s)	222, 883
LweI	GCATC	6 cut(s)	155, 750, 960, 1006, 1226, 1733
MaeI	CTAG	3 cut(s)	132, 1176, 1467
MaeII	ACGT	4 cut(s)	81, 308, 1202, 1510
MaeIII	GTNAC	2 cut(s)	1594, 1703
MalI	GATC	3 cut(s)	780, 892, 1284
MbiI	CCGCTC	1 cut(s)	104
MboI	GATC	3 cut(s)	778, 890, 1282
MfeI	CAATTG	1 cut(s)	864
MflI	RGATCY	2 cut(s)	778, 1282
MhlI	GDGCHC	2 cut(s)	598, 844
MluCI	AATT	9 cut(s)	19, 328, 430, 510, 750, 864, 1179, 1239, 1320
MlyI	GAGTC	1 cut(s)	101
MmeI	TCCRAC	3 cut(s)	374, 1260, 1323
Mph1103I	ATGCAT	2 cut(s)	706, 1219
MroI	TCCGGA	1 cut(s)	1160
MroNI	GCCGGC	1 cut(s)	947
MroXI	GAANNNNTTC	2 cut(s)	44, 1168
MspA1I	CMGCKG	1 cut(s)	896
MspCI	CTTAAG	2 cut(s)	353, 1249
MspI	CCGG	3 cut(s)	948, 1040, 1161
MspR9I	CCNGG	5 cut(s)	592, 927, 1094, 1439, 1715
MunI	CAATTG	1 cut(s)	864
MvaI	CCWGG	5 cut(s)	592, 927, 1094, 1439, 1715
MwoI	GCNNNNNNNGC	6 cut(s)	137, 232, 319, 944, 1154, 1223
NaeI	GCCGGC	1 cut(s)	949
NcoI	CCATGG	1 cut(s)	207
NdeI	CATATG	1 cut(s)	652
NdeII	GATC	3 cut(s)	778, 890, 1282
NgoMIV	GCCGGC	1 cut(s)	947
NheI	GCTAGC	2 cut(s)	131, 1466
NlaIII	CATG	7 cut(s)	121, 211, 488, 524, 559, 633, 1761
NlaIV	GGNNCC	6 cut(s)	350, 780, 801, 1038, 1152, 1719
NsbI	TGCGCA	1 cut(s)	710
NsiI	ATGCAT	2 cut(s)	706, 1219
NspI	RCATGY	1 cut(s)	524
NspV	TTCGAA	1 cut(s)	57
PaeR7I	CTCGAG	1 cut(s)	273
PaqCI	CACCTGC	1 cut(s)	1627
PceI	AGGCCT	1 cut(s)	1697
PciSI	GCTCTTC	1 cut(s)	94
PcsI	WCGNNNNNNNCGW	2 cut(s)	54, 1384
PdiI	GCCGGC	1 cut(s)	949
PdmI	GAANNNNTTC	2 cut(s)	44, 1168
PfeI	GAWTC	6 cut(s)	370, 610, 1123, 1169, 1552, 1651
PflFI	GACNNNGTC	1 cut(s)	307
PflMI	CCANNNNNTGG	1 cut(s)	1420
PfoI	TCCNGGA	1 cut(s)	1713
PkrI	GCNGC	4 cut(s)	214, 237, 898, 1021
PleI	GAGTC	1 cut(s)	101
PpsI	GAGTC	1 cut(s)	101
PpuMI	RGGWCCY	3 cut(s)	124, 349, 1717
PshBI	ATTAAT	1 cut(s)	1242
Psp1406I	AACGTT	2 cut(s)	81, 1202
Psp5II	RGGWCCY	3 cut(s)	124, 349, 1717
Psp6I	CCWGG	5 cut(s)	590, 925, 1092, 1437, 1713
PspFI	CCCAGC	1 cut(s)	1487
PspGI	CCWGG	5 cut(s)	590, 925, 1092, 1437, 1713
PspN4I	GGNNCC	6 cut(s)	350, 780, 801, 1038, 1152, 1719
PspPI	GGNCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
PspPPI	RGGWCCY	3 cut(s)	124, 349, 1717
PspXI	VCTCGAGB	1 cut(s)	273
PstNI	CAGNNNCTG	1 cut(s)	899
PsuI	RGATCY	2 cut(s)	778, 1282
PsyI	GACNNNGTC	1 cut(s)	307
PvuII	CAGCTG	1 cut(s)	896
RsaI	GTAC	3 cut(s)	692, 994, 1051
RsaNI	GTAC	3 cut(s)	691, 993, 1050
SapI	GCTCTTC	1 cut(s)	94
SatI	GCNGC	4 cut(s)	213, 236, 897, 1020
Sau3AI	GATC	3 cut(s)	778, 890, 1282
Sau96I	GGNCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
SchI	GAGTC	1 cut(s)	101
ScrFI	CCNGG	5 cut(s)	592, 927, 1094, 1439, 1715
SduI	GDGCHC	2 cut(s)	598, 844
SfaNI	GCATC	6 cut(s)	155, 750, 960, 1006, 1226, 1733
SfcI	CTRYAG	1 cut(s)	1031
Sfr274I	CTCGAG	1 cut(s)	273
SfuI	TTCGAA	1 cut(s)	57
SinI	GGWCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
SlaI	CTCGAG	1 cut(s)	273
SmlI	CTYRAG	5 cut(s)	273, 314, 353, 834, 1249
SmoI	CTYRAG	5 cut(s)	273, 314, 353, 834, 1249
Sse9I	AATT	9 cut(s)	19, 328, 430, 510, 750, 864, 1179, 1239, 1320
SseBI	AGGCCT	1 cut(s)	1697
SsiI	CCGC	7 cut(s)	104, 213, 224, 1020, 1105, 1148, 1418
SspMI	CTAG	3 cut(s)	132, 1176, 1467
StuI	AGGCCT	1 cut(s)	1697
StyD4I	CCNGG	5 cut(s)	590, 925, 1092, 1437, 1713
StyI	CCWWGG	1 cut(s)	207
TaaI	ACNGT	8 cut(s)	194, 202, 362, 647, 825, 1054, 1066, 1600
TaiI	ACGT	4 cut(s)	84, 311, 1205, 1513
TaqI	TCGA	6 cut(s)	57, 274, 457, 1288, 1387, 1743
TasI	AATT	9 cut(s)	19, 328, 430, 510, 750, 864, 1179, 1239, 1320
TatI	WGTACW	2 cut(s)	992, 1049
TauI	GCSGC	2 cut(s)	215, 1022
TfiI	GAWTC	6 cut(s)	370, 610, 1123, 1169, 1552, 1651
TscAI	CASTG	2 cut(s)	381, 1125
TseI	GCWGC	2 cut(s)	235, 896
TspDTI	ATGAA	7 cut(s)	106, 141, 501, 523, 729, 737, 1665
TspGWI	ACGGA	1 cut(s)	1059
TspRI	CASTG	2 cut(s)	381, 1125
Tth111I	GACNNNGTC	1 cut(s)	307
Van91I	CCANNNNNTGG	1 cut(s)	1420
Vha464I	CTTAAG	2 cut(s)	353, 1249
VpaK11BI	GGWCC	7 cut(s)	124, 349, 932, 1037, 1076, 1150, 1717
VspI	ATTAAT	1 cut(s)	1242
XceI	RCATGY	1 cut(s)	524
XhoI	CTCGAG	1 cut(s)	273
XmiI	GTMKAC	1 cut(s)	820
XmnI	GAANNNNTTC	2 cut(s)	44, 1168
XspI	CTAG	3 cut(s)	132, 1176, 1467
Zsp2I	ATGCAT	2 cut(s)	706, 1219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.