Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004074

Physical Location & Seq

Genomic Coordinates Forward (+)

23631047 .. 23644235

Gene Structure 13189 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1230600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1197 bp

ATGGATGTCCTCGCTGGCTCTGCAACTGCTTCTTCTTCATACCCTCATTTACTAATCTCCAACTTCTACAAGAACCACTGTAGCTCCGCCTCCATGGCCGCCGCTCCTCCACCTCAGTTGCTGCAGTGGAAGTATCTGCCAGTAAGGATTGTAGAAGCTCGAGCGAAGTCTCCCTTAATGTGTATAATGACTATGTCGCTGAAGAGACCAATAAATTATGCAAATTATAGCAAGATCAAGAAAAAACTTCGATTGAAGAAATATGCTGGCAGTCTCCATGAAGTGATAAAATGTTCAGAAAAAATCAGACGACATATTTTTGTTTTTGTTGCTGGGCTGATGGTTGTCATGTCAGCTTCCCTTTCTGTTAGCAGTATTCCATCCTGGGCTCTCACAGAAGAGAATCTTCTCTTCTTAGAGGCATGGAAGATGATTGACCGTGCATATGTTGACAAAAGTTTCAATGGACAAAGTTGGTTTCGGTACAGAGAAAATGCACTGCGCAATGAACCGATGAATGCACGAGAAGAGACATATATGGCAATTAGGAAGATGCTTGCCACATTGGAGGATCCTTTCACTCGGTTTCTGGAGCCTGAAAAGTTAACGAGTCTACGGTCTGGAACTCGAGGTGCTCTTACAGGCGTAGGGCTGTCAATTGGCTACCCTACACAACTTGATGGATCAGCTGCTGGTCTTGTTGTTATCTCAGCTTCCGCAGGAGGTCCAGCAAACAGGGCTGGCATCTTGTCTGGGGATGTTATTCTGGCGATTGATGATACAAGTACAGAAACAATGGGCATATATGATGCAGCAGAGCGGCTACAGGGATCTGAAGGAAGTTCAGTGAAACTAACCATTCGGAGTGGACCTGAAATAAAGCACCTGGATCTAATGCGGGAGAAAGTTTCACTGAATCCTGTGAAGTCAAGACTATGTGCGGTGCCTGCTTCGGGAAAGGATTCCCCTAGGATTGGATATATCAAACTACCAACGTTCAATCAAAATGCATCTGGTGCTGTCAAGGAAGCAATTAATACCTTAAGGAGTAATAATGTTAATGCCTTTGTTGGTGATAGTCGTGGTGTTCGAGATATATATGACACCGATGGAAGCAAAGCAGTAGCGACTTCAGAACCCCTAGTTGTGCTGGTACATGTTTATTTAGTAATACGATTAAGTAGATTTGGTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009579 GO:0016787 GO:0019538 GO:0031977 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044436 GO:0044444 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ PDZ_2 Peptidase_S41

Protein Analysis

399

Amino Acids

43.61

Weight (kDa)

9.59

Isoelectric Point (pI)

43.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PDZ	PF00595	216 - 288	3.8e-09	PDZ domain
PDZ_2	PF13180	229 - 295	2.3e-06	PDZ domain
PDZ_6	PF17820	235 - 288	3.2e-12	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17740 AT4G17740
fragaria_vesca	FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180 FvH4_4g05180
malus_domestica	MD13G1225700.v1.1 MD16G1230600.v1.1
prunus_persica	Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1 Prupe.1G052500_v2.0.a1
pyrus_communis	pycom13g19890 pycom16g19260
rosa_chinensis	RchiOBHm_Chr4g0396091
rosa_laevigata	RLG00000009573
rosa_multiflora	Rmu_sc0006325.1_g000018 Rmu_sc0007010.1_g000014 Rmu_sc0009634.1_g000011
rosa_roxburghii	Rroxscaffold_5G00340890
rosa_rugosa	Rorug03G0352600 Rorug03G0352600
rosa_samantha	Rh4BG064100 Rh4CG070700 Rh4DG061100
rosa_wichuraiana	Rw4G005320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	503
AccB1I	GGYRCC	1 cut(s)	943
AccBSI	CCGCTC	2 cut(s)	104, 820
AccI	GTMKAC	1 cut(s)	613
AciI	CCGC	7 cut(s)	87, 99, 102, 717, 820, 898, 941
AclI	AACGTT	1 cut(s)	995
AclWI	GGATC	5 cut(s)	566, 579, 691, 838, 897
AcoI	YGGCCR	1 cut(s)	96
AcuI	CTGAAG	3 cut(s)	221, 855, 1116
AfaI	GTAC	3 cut(s)	485, 787, 1155
AfiI	CCNNNNNNNGG	2 cut(s)	953, 974
AflII	CTTAAG	1 cut(s)	1042
AflIII	ACRYGT	1 cut(s)	1156
AgsI	TTSAA	3 cut(s)	256, 463, 1000
AjnI	CCWGG	2 cut(s)	383, 885
AluBI	AGCT	5 cut(s)	84, 158, 356, 689, 713
AluI	AGCT	5 cut(s)	84, 158, 356, 689, 713
Alw21I	GWGCWC	1 cut(s)	637
Alw26I	GTCTC	4 cut(s)	174, 199, 278, 524
AlwI	GGATC	5 cut(s)	566, 579, 691, 838, 897
AlwNI	CAGNNNCTG	2 cut(s)	121, 692
Ama87I	CYCGRG	2 cut(s)	159, 627
AoxI	GGCC	1 cut(s)	96
ApeKI	GCWGC	3 cut(s)	121, 689, 812
AseI	ATTAAT	1 cut(s)	1035
Asp700I	GAANNNNTTC	1 cut(s)	961
AspA2I	CCTAGG	1 cut(s)	968
AspLEI	GCGC	1 cut(s)	504
AspS9I	GGNCC	2 cut(s)	725, 869
AsuHPI	GGTGA	1 cut(s)	1085
AvaI	CYCGRG	2 cut(s)	159, 627
AvaII	GGWCC	2 cut(s)	725, 869
AvrII	CCTAGG	1 cut(s)	968
BamHI	GGATCC	1 cut(s)	571
BanI	GGYRCC	1 cut(s)	943
BanII	GRGCYC	1 cut(s)	391
BauI	CACGAG	1 cut(s)	522
Bbv12I	GWGCWC	1 cut(s)	637
BbvI	GCAGC	3 cut(s)	108, 676, 824
BccI	CCATC	4 cut(s)	334, 388, 674, 1103
BciT130I	CCWGG	2 cut(s)	385, 887
BcoDI	GTCTC	4 cut(s)	174, 199, 278, 524
BfaI	CTAG	2 cut(s)	969, 1142
BfmI	CTRYAG	3 cut(s)	79, 122, 824
BfrI	CTTAAG	1 cut(s)	1042
BglI	GCCNNNNNGGC	1 cut(s)	95
BisI	GCNGC	6 cut(s)	99, 102, 122, 690, 813, 821
BlnI	CCTAGG	1 cut(s)	968
BlsI	GCNGC	6 cut(s)	100, 103, 123, 691, 814, 822
Bme1390I	CCNGG	2 cut(s)	385, 887
Bme18I	GGWCC	2 cut(s)	725, 869
BmeT110I	CYCGRG	2 cut(s)	159, 627
BmgT120I	GGNCC	2 cut(s)	725, 869
BmiI	GGNNCC	3 cut(s)	573, 594, 945
BmrFI	CCNGG	2 cut(s)	385, 887
BmsI	GCATC	4 cut(s)	543, 753, 799, 1019
BpmI	CTGGAG	1 cut(s)	611
BsaI	GGTCTC	1 cut(s)	199
BsaJI	CCNNGG	3 cut(s)	93, 384, 968
BsaXI	ACNNNNNCTCC	2 cut(s)	68, 98
Bsc4I	CCNNNNNNNGG	2 cut(s)	953, 974
Bse1I	ACTGG	1 cut(s)	140
Bse3DI	GCAATG	1 cut(s)	511
BseBI	CCWGG	2 cut(s)	385, 887
BseDI	CCNNGG	3 cut(s)	93, 384, 968
BseGI	GGATG	3 cut(s)	10, 380, 763
BseLI	CCNNNNNNNGG	2 cut(s)	953, 974
BseMI	GCAATG	1 cut(s)	511
BseMII	CTCAG	2 cut(s)	128, 723
BseNI	ACTGG	1 cut(s)	140
BseRI	GAGGAG	1 cut(s)	96
BseXI	GCAGC	3 cut(s)	108, 676, 824
BseYI	CCCAGC	1 cut(s)	332
BshFI	GGCC	1 cut(s)	98
BshNI	GGYRCC	1 cut(s)	943
BsiHKAI	GWGCWC	1 cut(s)	637
BsiHKCI	CYCGRG	2 cut(s)	159, 627
BslI	CCNNNNNNNGG	2 cut(s)	953, 974
BsmAI	GTCTC	4 cut(s)	174, 199, 278, 524
BsmI	GAATGC	1 cut(s)	523
BsnI	GGCC	1 cut(s)	98
Bso31I	GGTCTC	1 cut(s)	199
BsoBI	CYCGRG	2 cut(s)	159, 627
Bsp1286I	GDGCHC	2 cut(s)	391, 637
Bsp143I	GATC	5 cut(s)	234, 571, 683, 830, 889
Bsp19I	CCATGG	1 cut(s)	93
BspACI	CCGC	7 cut(s)	87, 99, 102, 717, 820, 898, 941
BspANI	GGCC	1 cut(s)	98
BspCNI	CTCAG	2 cut(s)	127, 722
BspLI	GGNNCC	3 cut(s)	573, 594, 945
BspMAI	CTGCAG	1 cut(s)	126
BspPI	GGATC	5 cut(s)	566, 579, 691, 838, 897
BspT107I	GGYRCC	1 cut(s)	943
BspTI	CTTAAG	1 cut(s)	1042
BspTNI	GGTCTC	1 cut(s)	199
BsrBI	CCGCTC	2 cut(s)	104, 820
BsrDI	GCAATG	1 cut(s)	511
BsrI	ACTGG	1 cut(s)	140
BssECI	CCNNGG	3 cut(s)	93, 384, 968
BssMI	GATC	5 cut(s)	234, 571, 683, 830, 889
BssSI	CACGAG	1 cut(s)	522
BssT1I	CCWWGG	2 cut(s)	93, 968
Bst2BI	CACGAG	1 cut(s)	522
Bst2UI	CCWGG	2 cut(s)	385, 887
Bst4CI	ACNGT	3 cut(s)	80, 440, 618
Bst6I	CTCTTC	4 cut(s)	197, 393, 416, 522
BstAFI	CTTAAG	1 cut(s)	1042
BstAPI	GCANNNNNTGC	1 cut(s)	1016
BstC8I	GCNNGC	5 cut(s)	16, 268, 558, 742, 948
BstDEI	CTNAG	3 cut(s)	114, 415, 709
BstDSI	CCRYGG	1 cut(s)	93
BstF5I	GGATG	3 cut(s)	10, 380, 763
BstHHI	GCGC	1 cut(s)	504
BstKTI	GATC	5 cut(s)	237, 574, 686, 833, 892
BstMAI	GTCTC	4 cut(s)	174, 199, 278, 524
BstMBI	GATC	5 cut(s)	234, 571, 683, 830, 889
BstMWI	GCNNNNNNNGC	5 cut(s)	20, 95, 737, 947, 1016
BstNI	CCWGG	2 cut(s)	385, 887
BstNSI	RCATGY	1 cut(s)	1160
BstSCI	CCNGG	2 cut(s)	383, 885
BstSFI	CTRYAG	3 cut(s)	79, 122, 824
BstV1I	GCAGC	3 cut(s)	108, 676, 824
BstX2I	RGATCY	3 cut(s)	571, 830, 889
BstYI	RGATCY	3 cut(s)	571, 830, 889
BsuRI	GGCC	1 cut(s)	98
BtgI	CCRYGG	1 cut(s)	93
BtsCI	GGATG	3 cut(s)	10, 380, 763
BtsI	GCAGTG	2 cut(s)	131, 497
BtsIMutI	CAGTG	5 cut(s)	76, 131, 497, 852, 911
Cac8I	GCNNGC	5 cut(s)	16, 268, 558, 742, 948
CaiI	CAGNNNCTG	2 cut(s)	121, 692
CfoI	GCGC	1 cut(s)	504
Cfr13I	GGNCC	2 cut(s)	725, 869
Csp6I	GTAC	3 cut(s)	484, 786, 1154
CspCI	CAANNNNNGTGG	2 cut(s)	99, 134
CviAII	CATG	5 cut(s)	94, 278, 349, 423, 1157
CviQI	GTAC	3 cut(s)	484, 786, 1154
DdeI	CTNAG	3 cut(s)	114, 415, 709
DpnI	GATC	5 cut(s)	236, 573, 685, 832, 891
DpnII	GATC	5 cut(s)	234, 571, 683, 830, 889
EaeI	YGGCCR	1 cut(s)	96
Eam1104I	CTCTTC	4 cut(s)	197, 393, 416, 522
EarI	CTCTTC	4 cut(s)	197, 393, 416, 522
EciI	GGCGGA	1 cut(s)	76
Eco130I	CCWWGG	2 cut(s)	93, 968
Eco24I	GRGCYC	1 cut(s)	391
Eco31I	GGTCTC	1 cut(s)	199
Eco47I	GGWCC	2 cut(s)	725, 869
Eco57I	CTGAAG	3 cut(s)	221, 855, 1116
Eco88I	CYCGRG	2 cut(s)	159, 627
EcoRII	CCWGG	2 cut(s)	383, 885
EcoT14I	CCWWGG	2 cut(s)	93, 968
EcoT22I	ATGCAT	1 cut(s)	1012
EcoT38I	GRGCYC	1 cut(s)	391
ErhI	CCWWGG	2 cut(s)	93, 968
FaeI	CATG	5 cut(s)	97, 281, 352, 426, 1160
FalI	AAGNNNNNCTT	4 cut(s)	158, 190, 390, 422
FatI	CATG	5 cut(s)	93, 277, 348, 422, 1156
FauI	CCCGC	1 cut(s)	891
FauNDI	CATATG	1 cut(s)	445
FblI	GTMKAC	1 cut(s)	613
Fnu4HI	GCNGC	6 cut(s)	99, 102, 122, 690, 813, 821
FokI	GGATG	3 cut(s)	17, 367, 770
FriOI	GRGCYC	1 cut(s)	391
Fsp4HI	GCNGC	6 cut(s)	99, 102, 122, 690, 813, 821
FspBI	CTAG	2 cut(s)	969, 1142
FspI	TGCGCA	1 cut(s)	503
GlaI	GCGC	1 cut(s)	503
GluI	GCNGC	6 cut(s)	99, 102, 122, 690, 813, 821
GsaI	CCCAGC	1 cut(s)	336
GsuI	CTGGAG	1 cut(s)	611
HaeIII	GGCC	1 cut(s)	98
HhaI	GCGC	1 cut(s)	504
Hin1II	CATG	5 cut(s)	97, 281, 352, 426, 1160
Hin6I	GCGC	1 cut(s)	502
HinP1I	GCGC	1 cut(s)	502
HincII	GTYRAC	2 cut(s)	451, 606
HindII	GTYRAC	2 cut(s)	451, 606
HinfI	GANTC	4 cut(s)	403, 610, 916, 962
HpaI	GTTAAC	1 cut(s)	606
HphI	GGTGA	1 cut(s)	1085
Hpy166II	GTNNAC	4 cut(s)	451, 606, 614, 869
Hpy188I	TCNGA	5 cut(s)	298, 308, 835, 864, 1135
Hpy188III	TCNNGA	6 cut(s)	238, 590, 621, 930, 954, 1091
Hpy8I	GTNNAC	4 cut(s)	451, 606, 614, 869
HpyAV	CCTTC	1 cut(s)	830
HpyCH4III	ACNGT	3 cut(s)	80, 440, 618
HpyCH4IV	ACGT	1 cut(s)	995
HpyCH4V	TGCA	8 cut(s)	23, 124, 221, 443, 497, 521, 812, 1010
HpyF10VI	GCNNNNNNNGC	5 cut(s)	20, 95, 737, 947, 1016
HpyF3I	CTNAG	3 cut(s)	114, 415, 709
HpySE526I	ACGT	1 cut(s)	995
Hsp92II	CATG	5 cut(s)	97, 281, 352, 426, 1160
HspAI	GCGC	1 cut(s)	502
KspAI	GTTAAC	1 cut(s)	606
Kzo9I	GATC	5 cut(s)	234, 571, 683, 830, 889
LmnI	GCTCC	3 cut(s)	89, 109, 592
Lsp1109I	GCAGC	3 cut(s)	108, 676, 824
LweI	GCATC	4 cut(s)	543, 753, 799, 1019
MaeI	CTAG	2 cut(s)	969, 1142
MaeII	ACGT	1 cut(s)	995
MalI	GATC	5 cut(s)	236, 573, 685, 832, 891
MbiI	CCGCTC	2 cut(s)	104, 820
MboI	GATC	5 cut(s)	234, 571, 683, 830, 889
MfeI	CAATTG	1 cut(s)	657
MflI	RGATCY	3 cut(s)	571, 830, 889
MhlI	GDGCHC	2 cut(s)	391, 637
MluCI	AATT	5 cut(s)	214, 223, 543, 657, 1032
MlyI	GAGTC	1 cut(s)	619
MmeI	TCCRAC	1 cut(s)	84
MnlI	CCTC	9 cut(s)	20, 54, 100, 117, 123, 412, 562, 623, 716
Mph1103I	ATGCAT	1 cut(s)	1012
MroXI	GAANNNNTTC	1 cut(s)	961
MseI	TTAA	6 cut(s)	176, 605, 1035, 1043, 1059, 1178
MspA1I	CMGCKG	1 cut(s)	689
MspCI	CTTAAG	1 cut(s)	1042
MspR9I	CCNGG	2 cut(s)	385, 887
MunI	CAATTG	1 cut(s)	657
Mva1269I	GAATGC	1 cut(s)	523
MvaI	CCWGG	2 cut(s)	385, 887
MwoI	GCNNNNNNNGC	5 cut(s)	20, 95, 737, 947, 1016
NcoI	CCATGG	1 cut(s)	93
NdeI	CATATG	1 cut(s)	445
NdeII	GATC	5 cut(s)	234, 571, 683, 830, 889
NlaIII	CATG	5 cut(s)	97, 281, 352, 426, 1160
NlaIV	GGNNCC	3 cut(s)	573, 594, 945
NsbI	TGCGCA	1 cut(s)	503
NsiI	ATGCAT	1 cut(s)	1012
NspI	RCATGY	1 cut(s)	1160
PaeR7I	CTCGAG	2 cut(s)	159, 627
PciI	ACATGT	1 cut(s)	1156
PcsI	WCGNNNNNNNCGW	1 cut(s)	1087
PctI	GAATGC	1 cut(s)	523
PdmI	GAANNNNTTC	1 cut(s)	961
PfeI	GAWTC	3 cut(s)	403, 916, 962
PflFI	GACNNNGTC	1 cut(s)	193
PkrI	GCNGC	6 cut(s)	100, 103, 123, 691, 814, 822
PleI	GAGTC	1 cut(s)	618
PpsI	GAGTC	1 cut(s)	618
PscI	ACATGT	1 cut(s)	1156
PshBI	ATTAAT	1 cut(s)	1035
Psp1406I	AACGTT	1 cut(s)	995
Psp6I	CCWGG	2 cut(s)	383, 885
PspFI	CCCAGC	1 cut(s)	332
PspGI	CCWGG	2 cut(s)	383, 885
PspN4I	GGNNCC	3 cut(s)	573, 594, 945
PspPI	GGNCC	2 cut(s)	725, 869
PspXI	VCTCGAGB	2 cut(s)	159, 627
PstI	CTGCAG	1 cut(s)	126
PstNI	CAGNNNCTG	2 cut(s)	121, 692
PsuI	RGATCY	3 cut(s)	571, 830, 889
PsyI	GACNNNGTC	1 cut(s)	193
PvuII	CAGCTG	1 cut(s)	689
RsaI	GTAC	3 cut(s)	485, 787, 1155
RsaNI	GTAC	3 cut(s)	484, 786, 1154
SaqAI	TTAA	6 cut(s)	176, 605, 1035, 1043, 1059, 1178
SatI	GCNGC	6 cut(s)	99, 102, 122, 690, 813, 821
Sau3AI	GATC	5 cut(s)	234, 571, 683, 830, 889
Sau96I	GGNCC	2 cut(s)	725, 869
SchI	GAGTC	1 cut(s)	619
ScrFI	CCNGG	2 cut(s)	385, 887
SduI	GDGCHC	2 cut(s)	391, 637
SfaNI	GCATC	4 cut(s)	543, 753, 799, 1019
SfcI	CTRYAG	3 cut(s)	79, 122, 824
Sfr274I	CTCGAG	2 cut(s)	159, 627
SinI	GGWCC	2 cut(s)	725, 869
SlaI	CTCGAG	2 cut(s)	159, 627
SmlI	CTYRAG	3 cut(s)	159, 627, 1042
SmoI	CTYRAG	3 cut(s)	159, 627, 1042
Sse9I	AATT	5 cut(s)	214, 223, 543, 657, 1032
SsiI	CCGC	7 cut(s)	87, 99, 102, 717, 820, 898, 941
SspMI	CTAG	2 cut(s)	969, 1142
StyD4I	CCNGG	2 cut(s)	383, 885
StyI	CCWWGG	2 cut(s)	93, 968
TaaI	ACNGT	3 cut(s)	80, 440, 618
TaiI	ACGT	1 cut(s)	998
TaqI	TCGA	4 cut(s)	160, 250, 628, 1090
TasI	AATT	5 cut(s)	214, 223, 543, 657, 1032
TatI	WGTACW	1 cut(s)	785
TauI	GCSGC	3 cut(s)	101, 104, 823
TfiI	GAWTC	3 cut(s)	403, 916, 962
Tru1I	TTAA	6 cut(s)	176, 605, 1035, 1043, 1059, 1178
Tru9I	TTAA	6 cut(s)	176, 605, 1035, 1043, 1059, 1178
TscAI	CASTG	5 cut(s)	83, 131, 504, 852, 918
TseI	GCWGC	3 cut(s)	121, 689, 812
TspDTI	ATGAA	4 cut(s)	27, 294, 522, 530
TspRI	CASTG	5 cut(s)	83, 131, 504, 852, 918
Tth111I	GACNNNGTC	1 cut(s)	193
Vha464I	CTTAAG	1 cut(s)	1042
VpaK11BI	GGWCC	2 cut(s)	725, 869
VspI	ATTAAT	1 cut(s)	1035
XceI	RCATGY	1 cut(s)	1160
XhoI	CTCGAG	2 cut(s)	159, 627
XmaJI	CCTAGG	1 cut(s)	968
XmiI	GTMKAC	1 cut(s)	613
XmnI	GAANNNNTTC	1 cut(s)	961
XspI	CTAG	2 cut(s)	969, 1142
Zsp2I	ATGCAT	1 cut(s)	1012

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.