Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006550

Physical Location & Seq

Genomic Coordinates Reverse (-)

25264978 .. 25270038

Gene Structure 5061 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1242400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGTCGCCAGCTGCAATCGAGCCGGCATTTCCCGAATCGTCGTCTTTGAGCTCCCCTGATGTTCCTGAGAGATCTGAGAATCCTCGATTCAAGGACCTTAGAGGCGTCCAATGGCGTATAAATCTTGGGATTTTGCCCTCTTCCTCTTCTTCCTCCACTGATGATCTTCGTCGCGTTTCAGCTGATTGTAGAAGAAGGTATGCTGGATTGAGAAGGCGCCTTCTGGTTGATCCACATCCCCAAAAGGATGGGAGTAATTCTCCTGATCTTGCCATAGACAATCCACTATCGCAAAACCCAGATAGCACATGGGGTCACTTTTTCCGGAGTGCCGAGTTGGAGAAAATGGTTGATCAGGATTTATCACGTTTATACCCAGAACATGGAAGCTACTTTCAAACTCCAGGATGCCAAGGCATGTTGAGAAGAATCTTGTTATTGTGGTGTCTTAGACATCCAGAGTGTGGTTACAGACAAGGAATGCATGAACTCTTGGCTCCTCTATTGTATGTTCTTCATTTTGATGTGGAGCGGCTATCTCAAGTGCGAAAGCTATATGAAGATCACTTCACTGATAAATTTGATGGTCTATCATTTCATGAAAATGATCTTACATATAATTTTGAGTTCAAAAAATTTCCTGATTCCATGGAAGATGAAAATGGCTCTGATGGAAGTACATTGAATGTTAAGAGTCTTGACGAGCTTGATCCCGAGATACAGACCATTGTAATGCTTAGTGATTCTTATGGTGCTGAAGGTGAATTGGGTATTGTCTTGTCCGAGAGATTTATGGAACACGATGCATACTGTATGTTTGATGCTCTAATGAGTGGGGCCCATGGTTCAGTTTCTATGGCAGAATTCTTCTCTCCCTCCCCTGCTGTTGGTTCTCATACTAATTTACCTCCTGTCATTGAAGCATCTGCTGGGTTGTATCATTTGCTGTCTCTTGTTGATTCATCTCTACACAGCCACCTTGTTGAGCTTGGTGTTGAACCCCAGTATTTTGCACTCCGCTGGCTACGGGTTTTATTTGGGCGTGAATTTTCACTTGCAAATTTATTGATAATTTGGGGTGAAATATTTGCATCTGATAATAGTAAATTAGACAAATGTTCTGAAGATGATGCAGCCAGCTTTGCCATCCTTACTTCACCTCGAGGAGCATATATAGCAGCCATGGCAGTTTCAATGTTACTTTATTTGAGATCTTCCCTTCTTGCCGCTGAGAATGCTACTGCGTGTCTTCAGAGATTGTTAAATTTTCCTGAGAACATAGATTTGAAGAAACTGATACAAAAGGCGAAGTCTTTGCAGGATCTTGCTTTGAAGAACAATAGCTCACCCTCATTACTTTTATATAGTGGGCCCTATGAACATAGTAAATCAATGAATGCAAGAGGTCATAGCCTTTCAGTCGATTCTGTTTCTCCAAAAACACCACTAAGTCTAGTACCTGAAAGCTACTGGGAAGAGAAGTGGAGAGTTCTGCACAGGGAAGAAGAACTAAGGCAAAATGATATGAAGAAACAGGTTCCAAGCCATAAAAAGCGATGGACAGAAAAAGTAAAGTTGAGCCTATCTAGAACAGAGTCTGAACCATCTCCATCAAAATCTGAGAATGGCAAAAAGAACCCCAGGTTTTCTGTTAGACGAAGGTTGCTGCAAGACCTTTCTCGAGAACTCAGCTCAGAGGAGGATATTGAGACATTGTGTTCCCATGAGGACAAACTCTCTTCAGAAGTAGAGGGCAACAGAGAAACTGGGTTCGGCAAGGACCTTAATTCTGCCACCGAGAACAGATGTTTCAATGGAGATCCAGCCAGCGAAGAAAATTCATCTGTTTTCTCAGATCCAACAAGTCCTTGTACTGGGGATAATGGTCATGAACTTGAATCAGAAAAAAGTAGCGTTGCATCAAATCTGTCCATTGATGAAAATGATGATAATTCACAGGATGTGTTGGAAGAGCCACCTCTTCCGGTTTCTGATCATCCCAAGGGTGTCTCTCAAACATCGGAGAGCAACAATCATTCTCCCGGAAACTCAGTGCCAGGAAAGGAACGTAACCTTCTTTCTGGTAAATTCCCGAGATTTTGGAAGTTTGGACGGAATGCAGCTGGTGAAGGGACATCCGAGAAAGGACACAATGCCAGTGAGGCTATGAAATCTCCCCGTTGTGAAGGTAATCAGAATGCAACAAGCTCTTCTGTGGCTGAGGGGTCCTGCAGCTCTTTAGTTAGCCACAAAGGAGAAGCTGTTGACCAGCATGTGGTGGGTACTTTAAGGAATCTTGGGCAGTCTATGATTGAAAATATTCAGATTATCGAGTCTGTTTTCCAGCAGGACCGGGGTGTTCAGGTTGGATCGTCGGAGAACTTATCAAAGAACACTATAGTTGGCAAAGGACAAGTTACGGCCATGGCAGCTCTAACAGAGCTTCGGAAGATCAGCAACCTTTTGTCAGAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RabGAP-TBC

Protein Analysis

826

Amino Acids

91.62

Weight (kDa)

5.3

Isoelectric Point (pI)

58.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RabGAP-TBC	PF00566	114 - 172	1.2e-12	Rab-GTPase-TBC domain
RabGAP-TBC	PF00566	265 - 365	7.4e-09	Rab-GTPase-TBC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006550)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29950 AT4G29950 AT4G29950 AT5G57210 AT5G57210
fragaria_vesca	FvH4_4g07350 FvH4_4g07350 FvH4_4g07350 FvH4_4g07350 FvH4_4g07350 FvH4_4g07350
malus_domestica	MD13G1242400.v1.1 MD16G1249600.v1.1
prunus_persica	Prupe.1G076400_v2.0.a1 Prupe.1G076400_v2.0.a1
pyrus_communis	pycom16g20900
rosa_chinensis	RchiOBHm_Chr4g0399721
rosa_laevigata	RLG00000009244
rosa_multiflora	Rmu_ssc0000118.1_g000009
rosa_roxburghii	Rroxscaffold_5G00344660
rosa_rugosa	Rorug04G0018500
rosa_samantha	Rh4AG096100 Rh4BG093600 Rh4CG104900 Rh4DG088900
rosa_wichuraiana	Rw4G007940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1161
AccB1I	GGYRCC	1 cut(s)	216
AccB7I	CCANNNNNTGG	3 cut(s)	383, 464, 2275
AccBSI	CCGCTC	1 cut(s)	532
AccII	CGCG	1 cut(s)	174
AccIII	TCCGGA	1 cut(s)	324
AciI	CCGC	3 cut(s)	532, 1018, 1227
AclWI	GGATC	6 cut(s)	224, 704, 1329, 1814, 1850, 2377
AcoI	YGGCCR	1 cut(s)	2421
AcsI	RAATTY	8 cut(s)	578, 635, 863, 1046, 1060, 1264, 1837, 2087
AcuI	CTGAAG	4 cut(s)	777, 1143, 1235, 1725
AcyI	GRCGYC	2 cut(s)	105, 217
AfaI	GTAC	4 cut(s)	679, 1458, 1873, 2284
AfiI	CCNNNNNNNGG	5 cut(s)	383, 464, 887, 1874, 2275
AjnI	CCWGG	3 cut(s)	403, 1640, 2056
Alw21I	GWGCWC	1 cut(s)	53
Alw26I	GTCTC	3 cut(s)	954, 1703, 2014
AlwI	GGATC	6 cut(s)	224, 704, 1329, 1814, 1850, 2377
AlwNI	CAGNNNCTG	1 cut(s)	1598
Ama87I	CYCGRG	4 cut(s)	713, 1161, 1680, 2092
Aor13HI	TCCGGA	1 cut(s)	324
AoxI	GGCC	3 cut(s)	837, 1370, 2421
ApaI	GGGCCC	2 cut(s)	841, 1374
ApeKI	GCWGC	7 cut(s)	11, 1133, 1178, 1666, 2120, 2232, 2429
ApoI	RAATTY	8 cut(s)	578, 635, 863, 1046, 1060, 1264, 1837, 2087
Asp700I	GAANNNNTTC	1 cut(s)	2319
AspLEI	GCGC	1 cut(s)	219
AspS9I	GGNCC	8 cut(s)	94, 837, 838, 1370, 1371, 1780, 2226, 2350
AsuC2I	CCSGG	2 cut(s)	2043, 2354
AsuHPI	GGTGA	5 cut(s)	773, 1091, 1149, 1338, 2138
AvaI	CYCGRG	4 cut(s)	713, 1161, 1680, 2092
AvaII	GGWCC	4 cut(s)	94, 1780, 2226, 2350
BaeGI	GKGCMC	2 cut(s)	841, 1374
BanI	GGYRCC	1 cut(s)	216
BanII	GRGCYC	3 cut(s)	53, 841, 1374
BbsI	GAAGAC	1 cut(s)	1241
Bbv12I	GWGCWC	1 cut(s)	53
BbvCI	CCTCAGC	1 cut(s)	2220
BbvI	GCAGC	6 cut(s)	1145, 1190, 1653, 2132, 2244, 2441
BccI	CCATC	7 cut(s)	242, 578, 665, 1154, 1551, 1612, 1618
BceAI	ACGGC	1 cut(s)	2436
BcgI	CGANNNNNNTGC	2 cut(s)	1297, 1331
BciT130I	CCWGG	3 cut(s)	405, 1642, 2058
BclI	TGATCA	2 cut(s)	352, 1993
BcnI	CCSGG	2 cut(s)	2043, 2354
BcoDI	GTCTC	3 cut(s)	954, 1703, 2014
BfaI	CTAG	2 cut(s)	1454, 1587
BfmI	CTRYAG	2 cut(s)	2230, 2397
BfoI	RGCGCY	1 cut(s)	220
BglI	GCCNNNNNGGC	1 cut(s)	2162
BglII	AGATCT	2 cut(s)	71, 1211
BisI	GCNGC	9 cut(s)	12, 533, 1134, 1179, 1227, 1667, 2121, 2233, 2430
BlsI	GCNGC	9 cut(s)	13, 534, 1135, 1180, 1228, 1668, 2122, 2234, 2431
Bme1390I	CCNGG	5 cut(s)	405, 1642, 2043, 2058, 2354
Bme18I	GGWCC	4 cut(s)	94, 1780, 2226, 2350
BmeT110I	CYCGRG	4 cut(s)	713, 1161, 1680, 2092
BmgT120I	GGNCC	8 cut(s)	94, 837, 838, 1370, 1371, 1780, 2226, 2350
BmiI	GGNNCC	7 cut(s)	218, 498, 838, 839, 1372, 1539, 2227
BmrFI	CCNGG	5 cut(s)	405, 1642, 2043, 2058, 2354
BmrI	ACTGGG	4 cut(s)	997, 1480, 1776, 1884
BmsI	GCATC	7 cut(s)	398, 793, 811, 932, 1100, 1120, 1928
BmuI	ACTGGG	4 cut(s)	997, 1480, 1776, 1884
BpiI	GAAGAC	1 cut(s)	1241
BplI	GAGNNNNNCTC	2 cut(s)	244, 276
BpmI	CTGGAG	1 cut(s)	387
Bpu10I	CCTNAGC	1 cut(s)	2220
BpuEI	CTTGAG	1 cut(s)	525
BpuMI	CCSGG	2 cut(s)	2043, 2354
BsaBI	GATNNNNATC	1 cut(s)	234
BsaHI	GRCGYC	2 cut(s)	105, 217
BsaJI	CCNNGG	8 cut(s)	412, 648, 841, 1182, 1640, 2001, 2353, 2424
BsaWI	WCCGGW	2 cut(s)	324, 1984
BsaXI	ACNNNNNCTCC	2 cut(s)	2247, 2277
Bsc4I	CCNNNNNNNGG	5 cut(s)	383, 464, 887, 1874, 2275
Bse118I	RCCGGY	1 cut(s)	22
Bse1I	ACTGG	5 cut(s)	1003, 1475, 1771, 1879, 2157
Bse8I	GATNNNNATC	1 cut(s)	234
BseAI	TCCGGA	1 cut(s)	324
BseBI	CCWGG	3 cut(s)	405, 1642, 2058
BseDI	CCNNGG	8 cut(s)	412, 648, 841, 1182, 1640, 2001, 2353, 2424
BseGI	GGATG	8 cut(s)	235, 253, 413, 454, 1146, 1966, 1996, 2135
BseJI	GATNNNNATC	1 cut(s)	234
BseLI	CCNNNNNNNGG	5 cut(s)	383, 464, 887, 1874, 2275
BseNI	ACTGG	5 cut(s)	1003, 1475, 1771, 1879, 2157
BseRI	GAGGAG	3 cut(s)	489, 1179, 1712
BseSI	GKGCMC	2 cut(s)	841, 1374
BseXI	GCAGC	6 cut(s)	1145, 1190, 1653, 2132, 2244, 2441
BseYI	CCCAGC	1 cut(s)	929
BsgI	GTGCAG	1 cut(s)	1478
Bsh1236I	CGCG	1 cut(s)	174
BshFI	GGCC	3 cut(s)	839, 1372, 2423
BshNI	GGYRCC	1 cut(s)	216
BsiHKAI	GWGCWC	1 cut(s)	53
BsiHKCI	CYCGRG	4 cut(s)	713, 1161, 1680, 2092
BsiSI	CCGG	5 cut(s)	23, 325, 1985, 2043, 2353
BslFI	GGGAC	1 cut(s)	2146
BslI	CCNNNNNNNGG	5 cut(s)	383, 464, 887, 1874, 2275
BsmAI	GTCTC	3 cut(s)	954, 1703, 2014
BsmFI	GGGAC	1 cut(s)	2146
BsmI	GAATGC	5 cut(s)	486, 1240, 1402, 2122, 2203
BsnI	GGCC	3 cut(s)	839, 1372, 2423
BsoBI	CYCGRG	4 cut(s)	713, 1161, 1680, 2092
Bsp120I	GGGCCC	2 cut(s)	837, 1370
Bsp1286I	GDGCHC	3 cut(s)	53, 841, 1374
Bsp13I	TCCGGA	1 cut(s)	324
Bsp19I	CCATGG	4 cut(s)	648, 841, 1182, 2424
BspACI	CCGC	3 cut(s)	532, 1018, 1227
BspANI	GGCC	3 cut(s)	839, 1372, 2423
BspEI	TCCGGA	1 cut(s)	324
BspFNI	CGCG	1 cut(s)	174
BspHI	TCATGA	2 cut(s)	598, 1888
BspLI	GGNNCC	7 cut(s)	218, 498, 838, 839, 1372, 1539, 2227
BspMAI	CTGCAG	1 cut(s)	2234
BspPI	GGATC	6 cut(s)	224, 704, 1329, 1814, 1850, 2377
BspQI	GCTCTTC	2 cut(s)	1965, 2215
BspT107I	GGYRCC	1 cut(s)	216
BsrBI	CCGCTC	1 cut(s)	532
BsrFI	RCCGGY	1 cut(s)	22
BsrI	ACTGG	5 cut(s)	1003, 1475, 1771, 1879, 2157
BssAI	RCCGGY	1 cut(s)	22
BssECI	CCNNGG	8 cut(s)	412, 648, 841, 1182, 1640, 2001, 2353, 2424
BssNI	GRCGYC	2 cut(s)	105, 217
BssT1I	CCWWGG	6 cut(s)	412, 648, 841, 1182, 2001, 2424
Bst2UI	CCWGG	3 cut(s)	405, 1642, 2058
Bst4CI	ACNGT	1 cut(s)	812
Bst6I	CTCTTC	7 cut(s)	145, 151, 1470, 1744, 1965, 1986, 2215
BstACI	GRCGYC	2 cut(s)	105, 217
BstC8I	GCNNGC	5 cut(s)	9, 24, 1022, 1138, 1828
BstDSI	CCRYGG	4 cut(s)	648, 841, 1182, 2424
BstF5I	GGATG	8 cut(s)	235, 253, 413, 454, 1146, 1966, 1996, 2135
BstFNI	CGCG	1 cut(s)	174
BstH2I	RGCGCY	1 cut(s)	220
BstHHI	GCGC	1 cut(s)	219
BstMAI	GTCTC	3 cut(s)	954, 1703, 2014
BstMWI	GCNNNNNNNGC	6 cut(s)	1142, 1175, 1184, 1235, 2162, 2429
BstNI	CCWGG	3 cut(s)	405, 1642, 2058
BstNSI	RCATGY	2 cut(s)	421, 2276
BstSCI	CCNGG	5 cut(s)	403, 1640, 2041, 2056, 2352
BstSFI	CTRYAG	2 cut(s)	2230, 2397
BstSLI	GKGCMC	2 cut(s)	841, 1374
BstUI	CGCG	1 cut(s)	174
BstV1I	GCAGC	6 cut(s)	1145, 1190, 1653, 2132, 2244, 2441
BstV2I	GAAGAC	1 cut(s)	1241
BstX2I	RGATCY	5 cut(s)	71, 1211, 1321, 1819, 1855
BstXI	CCANNNNNNTGG	1 cut(s)	248
BstYI	RGATCY	5 cut(s)	71, 1211, 1321, 1819, 1855
BsuRI	GGCC	3 cut(s)	839, 1372, 2423
BtgI	CCRYGG	4 cut(s)	648, 841, 1182, 2424
BtgZI	GCGATG	1 cut(s)	1570
BtsCI	GGATG	8 cut(s)	235, 253, 413, 454, 1146, 1966, 1996, 2135
BtsIMutI	CAGTG	4 cut(s)	156, 570, 2058, 2164
Cac8I	GCNNGC	5 cut(s)	9, 24, 1022, 1138, 1828
CaiI	CAGNNNCTG	1 cut(s)	1598
CciI	TCATGA	2 cut(s)	598, 1888
CfoI	GCGC	1 cut(s)	219
Cfr10I	RCCGGY	1 cut(s)	22
Cfr13I	GGNCC	8 cut(s)	94, 837, 838, 1370, 1371, 1780, 2226, 2350
CseI	GACGC	1 cut(s)	94
Csp6I	GTAC	4 cut(s)	678, 1457, 1872, 2283
CviQI	GTAC	4 cut(s)	678, 1457, 1872, 2283
DinI	GGCGCC	1 cut(s)	218
EaeI	YGGCCR	1 cut(s)	2421
Eam1104I	CTCTTC	7 cut(s)	145, 151, 1470, 1744, 1965, 1986, 2215
EarI	CTCTTC	7 cut(s)	145, 151, 1470, 1744, 1965, 1986, 2215
Ecl136II	GAGCTC	1 cut(s)	51
Eco130I	CCWWGG	6 cut(s)	412, 648, 841, 1182, 2001, 2424
Eco24I	GRGCYC	3 cut(s)	53, 841, 1374
Eco47I	GGWCC	4 cut(s)	94, 1780, 2226, 2350
Eco53kI	GAGCTC	1 cut(s)	51
Eco57I	CTGAAG	4 cut(s)	777, 1143, 1235, 1725
Eco88I	CYCGRG	4 cut(s)	713, 1161, 1680, 2092
EcoICRI	GAGCTC	1 cut(s)	51
EcoO109I	RGGNCCY	5 cut(s)	94, 837, 1371, 1780, 2226
EcoRI	GAATTC	1 cut(s)	863
EcoRII	CCWGG	3 cut(s)	403, 1640, 2056
EcoT14I	CCWWGG	6 cut(s)	412, 648, 841, 1182, 2001, 2424
EcoT22I	ATGCAT	2 cut(s)	486, 808
EcoT38I	GRGCYC	3 cut(s)	53, 841, 1374
EgeI	GGCGCC	1 cut(s)	218
EheI	GGCGCC	1 cut(s)	218
ErhI	CCWWGG	6 cut(s)	412, 648, 841, 1182, 2001, 2424
FaqI	GGGAC	1 cut(s)	2146
FbaI	TGATCA	2 cut(s)	352, 1993
Fnu4HI	GCNGC	9 cut(s)	12, 533, 1134, 1179, 1227, 1667, 2121, 2233, 2430
FokI	GGATG	8 cut(s)	222, 260, 420, 441, 1133, 1973, 1983, 2122
FriOI	GRGCYC	3 cut(s)	53, 841, 1374
Fsp4HI	GCNGC	9 cut(s)	12, 533, 1134, 1179, 1227, 1667, 2121, 2233, 2430
FspBI	CTAG	2 cut(s)	1454, 1587
GlaI	GCGC	1 cut(s)	218
GluI	GCNGC	9 cut(s)	12, 533, 1134, 1179, 1227, 1667, 2121, 2233, 2430
GsaI	CCCAGC	1 cut(s)	933
GsuI	CTGGAG	1 cut(s)	387
HaeII	RGCGCY	1 cut(s)	220
HaeIII	GGCC	3 cut(s)	839, 1372, 2423
HapII	CCGG	5 cut(s)	23, 325, 1985, 2043, 2353
HgaI	GACGC	1 cut(s)	94
HhaI	GCGC	1 cut(s)	219
Hin1I	GRCGYC	2 cut(s)	105, 217
Hin6I	GCGC	1 cut(s)	217
HinP1I	GCGC	1 cut(s)	217
HincII	GTYRAC	1 cut(s)	2266
HindII	GTYRAC	1 cut(s)	2266
HpaII	CCGG	5 cut(s)	23, 325, 1985, 2043, 2353
HphI	GGTGA	5 cut(s)	773, 1091, 1149, 1338, 2138
Hpy166II	GTNNAC	1 cut(s)	2266
Hpy8I	GTNNAC	1 cut(s)	2266
Hpy99I	CGWCG	3 cut(s)	43, 174, 2377
HpyAV	CCTTC	9 cut(s)	189, 207, 230, 752, 1229, 1653, 2084, 2123, 2180
HpyCH4III	ACNGT	1 cut(s)	812
HpyCH4IV	ACGT	2 cut(s)	367, 2068
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1142, 1175, 1184, 1235, 2162, 2429
HpySE526I	ACGT	2 cut(s)	367, 2068
Hsp92I	GRCGYC	2 cut(s)	105, 217
HspAI	GCGC	1 cut(s)	217
KasI	GGCGCC	1 cut(s)	216
Kpn2I	TCCGGA	1 cut(s)	324
KroI	GCCGGC	1 cut(s)	22
KroNI	GCCGGC	1 cut(s)	24
Ksp22I	TGATCA	2 cut(s)	352, 1993
LguI	GCTCTTC	2 cut(s)	1965, 2215
LmnI	GCTCC	4 cut(s)	56, 502, 529, 1166
Lsp1109I	GCAGC	6 cut(s)	1145, 1190, 1653, 2132, 2244, 2441
LweI	GCATC	7 cut(s)	398, 793, 811, 932, 1100, 1120, 1928
MaeI	CTAG	2 cut(s)	1454, 1587
MaeII	ACGT	2 cut(s)	367, 2068
MaeIII	GTNAC	5 cut(s)	314, 467, 1197, 2069, 2416
MbiI	CCGCTC	1 cut(s)	532
MflI	RGATCY	5 cut(s)	71, 1211, 1321, 1819, 1855
MhlI	GDGCHC	3 cut(s)	53, 841, 1374
Mly113I	GGCGCC	1 cut(s)	217
MlyI	GAGTC	3 cut(s)	703, 1604, 2342
MmeI	TCCRAC	5 cut(s)	318, 1883, 1947, 2347, 2355
Mph1103I	ATGCAT	2 cut(s)	486, 808
MroI	TCCGGA	1 cut(s)	324
MroNI	GCCGGC	1 cut(s)	22
MroXI	GAANNNNTTC	1 cut(s)	2319
MseI	TTAA	4 cut(s)	690, 1262, 1785, 2288
MslI	CAYNNNNRTG	3 cut(s)	522, 603, 731
MspA1I	CMGCKG	5 cut(s)	11, 182, 1020, 1229, 2123
MspI	CCGG	5 cut(s)	23, 325, 1985, 2043, 2353
MspR9I	CCNGG	5 cut(s)	405, 1642, 2043, 2058, 2354
Mva1269I	GAATGC	5 cut(s)	486, 1240, 1402, 2122, 2203
MvaI	CCWGG	3 cut(s)	405, 1642, 2058
MvnI	CGCG	1 cut(s)	174
MwoI	GCNNNNNNNGC	6 cut(s)	1142, 1175, 1184, 1235, 2162, 2429
NaeI	GCCGGC	1 cut(s)	24
NarI	GGCGCC	1 cut(s)	217
NciI	CCSGG	2 cut(s)	2043, 2354
NcoI	CCATGG	4 cut(s)	648, 841, 1182, 2424
NgoMIV	GCCGGC	1 cut(s)	22
NlaIV	GGNNCC	7 cut(s)	218, 498, 838, 839, 1372, 1539, 2227
NmeAIII	GCCGAG	1 cut(s)	358
NmuCI	GTSAC	1 cut(s)	314
NsiI	ATGCAT	2 cut(s)	486, 808
NspI	RCATGY	2 cut(s)	421, 2276
PaeR7I	CTCGAG	2 cut(s)	1161, 1680
PagI	TCATGA	2 cut(s)	598, 1888
PciSI	GCTCTTC	2 cut(s)	1965, 2215
PctI	GAATGC	5 cut(s)	486, 1240, 1402, 2122, 2203
PdiI	GCCGGC	1 cut(s)	24
PdmI	GAANNNNTTC	1 cut(s)	2319
PflMI	CCANNNNNTGG	3 cut(s)	383, 464, 2275
PfoI	TCCNGGA	2 cut(s)	403, 2041
PkrI	GCNGC	9 cut(s)	13, 534, 1135, 1180, 1228, 1668, 2122, 2234, 2431
PleI	GAGTC	3 cut(s)	702, 1603, 2341
PluTI	GGCGCC	1 cut(s)	220
PpsI	GAGTC	3 cut(s)	702, 1603, 2341
PpuMI	RGGWCCY	3 cut(s)	94, 1780, 2226
Psp124BI	GAGCTC	1 cut(s)	53
Psp5II	RGGWCCY	3 cut(s)	94, 1780, 2226
Psp6I	CCWGG	3 cut(s)	403, 1640, 2056
PspFI	CCCAGC	1 cut(s)	929
PspGI	CCWGG	3 cut(s)	403, 1640, 2056
PspN4I	GGNNCC	7 cut(s)	218, 498, 838, 839, 1372, 1539, 2227
PspOMI	GGGCCC	2 cut(s)	837, 1370
PspPI	GGNCC	8 cut(s)	94, 837, 838, 1370, 1371, 1780, 2226, 2350
PspPPI	RGGWCCY	3 cut(s)	94, 1780, 2226
PspXI	VCTCGAGB	1 cut(s)	1161
PstI	CTGCAG	1 cut(s)	2234
PstNI	CAGNNNCTG	1 cut(s)	1598
PsuI	RGATCY	5 cut(s)	71, 1211, 1321, 1819, 1855
PvuII	CAGCTG	3 cut(s)	11, 182, 2123
RsaI	GTAC	4 cut(s)	679, 1458, 1873, 2284
RsaNI	GTAC	4 cut(s)	678, 1457, 1872, 2283
RseI	CAYNNNNRTG	3 cut(s)	522, 603, 731
SacI	GAGCTC	1 cut(s)	53
SapI	GCTCTTC	2 cut(s)	1965, 2215
SaqAI	TTAA	4 cut(s)	690, 1262, 1785, 2288
SatI	GCNGC	9 cut(s)	12, 533, 1134, 1179, 1227, 1667, 2121, 2233, 2430
Sau96I	GGNCC	8 cut(s)	94, 837, 838, 1370, 1371, 1780, 2226, 2350
SchI	GAGTC	3 cut(s)	703, 1604, 2342
ScrFI	CCNGG	5 cut(s)	405, 1642, 2043, 2058, 2354
SduI	GDGCHC	3 cut(s)	53, 841, 1374
SfaNI	GCATC	7 cut(s)	398, 793, 811, 932, 1100, 1120, 1928
SfcI	CTRYAG	2 cut(s)	2230, 2397
SfoI	GGCGCC	1 cut(s)	218
Sfr274I	CTCGAG	2 cut(s)	1161, 1680
SinI	GGWCC	4 cut(s)	94, 1780, 2226, 2350
SlaI	CTCGAG	2 cut(s)	1161, 1680
SmiMI	CAYNNNNRTG	3 cut(s)	522, 603, 731
SmlI	CTYRAG	3 cut(s)	540, 1161, 1680
SmoI	CTYRAG	3 cut(s)	540, 1161, 1680
SsiI	CCGC	3 cut(s)	532, 1018, 1227
SspDI	GGCGCC	1 cut(s)	216
SspI	AATATT	2 cut(s)	1086, 2320
SspMI	CTAG	2 cut(s)	1454, 1587
SstI	GAGCTC	1 cut(s)	53
StyD4I	CCNGG	5 cut(s)	403, 1640, 2041, 2056, 2352
StyI	CCWWGG	6 cut(s)	412, 648, 841, 1182, 2001, 2424
TaaI	ACNGT	1 cut(s)	812
TaiI	ACGT	2 cut(s)	370, 2071
TaqI	TCGA	6 cut(s)	18, 85, 1162, 1422, 1681, 2331
TatI	WGTACW	2 cut(s)	677, 1871
TauI	GCSGC	2 cut(s)	535, 1229
Tru1I	TTAA	4 cut(s)	690, 1262, 1785, 2288
Tru9I	TTAA	4 cut(s)	690, 1262, 1785, 2288
TscAI	CASTG	4 cut(s)	163, 577, 2058, 2164
TseFI	GTSAC	1 cut(s)	314
TseI	GCWGC	7 cut(s)	11, 1133, 1178, 1666, 2120, 2232, 2429
Tsp45I	GTSAC	1 cut(s)	314
TspGWI	ACGGA	1 cut(s)	2128
TspRI	CASTG	4 cut(s)	163, 577, 2058, 2164
Van91I	CCANNNNNTGG	3 cut(s)	383, 464, 2275
VpaK11BI	GGWCC	4 cut(s)	94, 1780, 2226, 2350
XapI	RAATTY	8 cut(s)	578, 635, 863, 1046, 1060, 1264, 1837, 2087
XbaI	TCTAGA	1 cut(s)	1586
XceI	RCATGY	2 cut(s)	421, 2276
XcmI	CCANNNNNNNNNTGG	1 cut(s)	306
XhoI	CTCGAG	2 cut(s)	1161, 1680
XmnI	GAANNNNTTC	1 cut(s)	2319
XspI	CTAG	2 cut(s)	1454, 1587
Zsp2I	ATGCAT	2 cut(s)	486, 808

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.