Rh4BG093600

Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006550
Physical Location & Seq
Reverse (-)
15992286 .. 15997979
5694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG093600.1

Sequence Viewer

Length: 2010 bp
ATGCATGAACTCTTGGCTCCTCTTCTATATGTTCTTCATGTTGACGTGGAGGGTCTATCTCAAGTGCGAAAGCTATATGAAGATCACTTCACTGATAAATTTGATGATCTATCTTATCATGAAAATGATTCGACTTACAATTTTGATTTAAAAAACCTTCCTGATTCCATGGAAGATGAGGATAGCTTGCAGGGAGATGCATCAAAAGTTAAGAGTCTGGATGAGCTTGATCCCAAGATACAGACCATTGTAATGCTCAGTGATGCTTATGGTGCTGAAGGTGAATTGGGTATTGTCTTATCTGAGAAATTTATGGAACACGATGCATACTGCATGTTTGATGCGTTAATGAGTGGGGTCAATGGTTCAGTTTCTATGGCAGAATTCTTCTCTCCCTCCCCTGCTGTTGGCTCTCAGACTAGCTTACCTCCTGTCATTGAAGCATCTGCTGCATTGTATCATTTGCTGTCTCTTGTTGATTCATCTCTACACAGCCACCTTGTTGAGCTTGGTGTTGAACCCCAGTATTTTGCACTCCGCTGGTTAAGGGTTTTATTTGGACGTGAATTTTCACTTGCAAATCTATTGATAATTTGGGATGAGATATTTCTGTCTGATAATTGTAAATTAGACAAAGGTGGTGAAGATGATGCAGGATCCAGCTTTAGCATCCTTAGTTCACCTCGAGGAGCATTTATTTCAGCCCTGGCAGTTTCCATGTTACTTCATTTGAGATCTTCCCTTCTTGCCACAGAGAATGCAACTGCATGTCTTCAACGGTTACTAAACTTTCCTGAGAATATTGATTTAAAGAAACTGATACAGAAGGCAACGTCATTGCAGGCTCTTGCTTTGGAGAACAATAGCTCATCCTCATTTCCTTCATATATGGGGCCATATGAGCATAGTAAATCAAAGGCTGTGAGAGGTCATAGTCTTTCAATCGACTCCGTTTCTCCAAAAACTCCATTAAGTTTGGTAAATGAAAGCTACTGGGAAGAGAAGTGGAGAGTTCTGCACAGGGAAGAAGAACTTAGGCAAGATATTTTGAAACAACAGGTTCCCAGCCAGAAGAAGCGATGGACTGAAAAAGTAAAATTGAGCCTATCTAGATCTGAATCTGCCCCATCTCCAGCAAAATGTGAGAATGGCAAAAAGACCCCTAGGTTTGCTGTTAGACGAAAGTTGCTGGAAGACCTCTCGAAAGTGCTCAGCTCAGAGGAAGATATTGAGAAATTAGGCACTCACAAAGACAAAGGCTCTTCAGAAGTTGTGGTTAACAAAGAAGATGGTGTCAGTAAGGATCTTACTTCTGCAAATGAAGACAGATGTTTGAATGGAAGTCCAGCTAGTGAAGAGAACTCATTTGTTGGCTCATATCCAGCAAGTCCTCTGAGTGGGGCAAATGATGAACCTGAATTAGAGAAAAGTAGTGTTGGATCAAACTTATCCTTTGATGAAAATAATGATAATCCAAATGATGATAATCCACAGTGTGTATCAGAAGGCCCACCTCGTCCGGTTTCTGATCCAGCTGAGGGTGTCTCTCAAGCATCCGAGTGCAGCAATCATTCAACGGGGAATTCAGTGACAGGAAAGGAACGAAAGCTTCTTTCTGGTAAATTCCAGAGGTTTTGGAAGTTGGGATGGAGTGGAGCTGGTGAAGGCACGTCCGAGAAAGGGGGCAACACCCTTGACGCTACAAAATCTATCAGGAGTGATGTTGGTCAGAATGCAGCAAGCTCTTCTATGGCTGAAGGGTCCAATTCTGTAGTTAGCTGCAAAGGGGAAACTGTGGACCAGAATAGGACGGGTACTTTGAGGAATCTTGGCCATTCTATGCTTGATCATATACAGGTAATCGAGTCTGCTTTCCAGCAAGATCGAGGTGTTCAAGTGGGATCAGTTGAGAACTGTTCAAAAAATACTTTAGTTGGCAAAGGACAAGTAACGGCCATTGCAGCTCTCAAGGAGCTTCGGAAGATCAGCAATCTTTTGTCAGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

669

Amino Acids

73.12

Weight (kDa)

5.03

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RabGAP-TBC PF00566 104 - 206 1.2e-11 Rab-GTPase-TBC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 684
AciI CCGC 1 cut(s) 538
AclWI GGATC 7 cut(s) 224, 651, 664, 1311, 1447, 1523, 1909
AcoI YGGCCR 2 cut(s) 1831, 1953
AcsI RAATTY 6 cut(s) 98, 308, 383, 566, 1582, 1622
AcuI CTGAAG 3 cut(s) 297, 1248, 1776
AdeI CACNNNGTG 1 cut(s) 1496
AfaI GTAC 1 cut(s) 1816
AfiI CCNNNNNNNGG 4 cut(s) 407, 1397, 1538, 1680
AgsI TTSAA 9 cut(s) 440, 518, 776, 942, 1051, 1336, 1575, 1895, 1920
AjiI CACGTC 3 cut(s) 46, 563, 1671
AjnI CCWGG 1 cut(s) 705
Alw21I GWGCWC 1 cut(s) 1212
Alw26I GTCTC 2 cut(s) 474, 1549
AlwI GGATC 7 cut(s) 224, 651, 664, 1311, 1447, 1523, 1909
Ama87I CYCGRG 1 cut(s) 684
AoxI GGCC 4 cut(s) 893, 1507, 1831, 1953
ApeKI GCWGC 5 cut(s) 449, 1563, 1736, 1779, 1961
ApoI RAATTY 6 cut(s) 98, 308, 383, 566, 1582, 1622
ArsI GACNNNNNNTTYG 2 cut(s) 1801, 1833
AspA2I CCTAGG 1 cut(s) 1163
AspS9I GGNCC 4 cut(s) 893, 1508, 1761, 1798
AsuHPI GGTGA 4 cut(s) 293, 653, 672, 1673
AvaI CYCGRG 1 cut(s) 684
AvaII GGWCC 2 cut(s) 1761, 1798
AvrII CCTAGG 1 cut(s) 1163
BalI TGGCCA 1 cut(s) 1833
BamHI GGATCC 1 cut(s) 656
BbsI GAAGAC 3 cut(s) 764, 1200, 1329
Bbv12I GWGCWC 1 cut(s) 1212
BbvCI CCTCAGC 1 cut(s) 1536
BbvI GCAGC 5 cut(s) 436, 1575, 1748, 1766, 1973
BccI CCATC 4 cut(s) 1074, 1135, 1283, 1641
BceAI ACGGC 1 cut(s) 1968
BciT130I CCWGG 1 cut(s) 707
BclI TGATCA 1 cut(s) 1846
BcoDI GTCTC 2 cut(s) 474, 1549
BfaI CTAG 4 cut(s) 420, 1110, 1164, 1350
BfmI CTRYAG 1 cut(s) 1770
BglII AGATCT 2 cut(s) 734, 1112
BisI GCNGC 5 cut(s) 450, 1564, 1737, 1780, 1962
BlnI CCTAGG 1 cut(s) 1163
BlpI GCTNAGC 1 cut(s) 1211
BlsI GCNGC 5 cut(s) 451, 1565, 1738, 1781, 1963
Bme1390I CCNGG 1 cut(s) 707
Bme18I GGWCC 2 cut(s) 1761, 1798
BmeT110I CYCGRG 1 cut(s) 684
BmgBI CACGTC 3 cut(s) 46, 563, 1671
BmgT120I GGNCC 4 cut(s) 893, 1508, 1761, 1798
BmiI GGNNCC 5 cut(s) 18, 658, 894, 1062, 1762
BmrFI CCNGG 1 cut(s) 707
BmrI ACTGGG 2 cut(s) 517, 1003
BmsI GCATC 9 cut(s) 187, 209, 253, 313, 331, 452, 640, 678, 1562
BmuI ACTGGG 2 cut(s) 517, 1003
BpiI GAAGAC 3 cut(s) 764, 1200, 1329
BplI GAGNNNNNCTC 2 cut(s) 1529, 1561
BpmI CTGGAG 1 cut(s) 1116
Bpu10I CCTNAGC 1 cut(s) 1536
Bpu1102I GCTNAGC 1 cut(s) 1211
BpuEI CTTGAG 3 cut(s) 45, 1533, 1952
BsaBI GATNNNNATC 2 cut(s) 1117, 1485
BsaJI CCNNGG 3 cut(s) 168, 705, 1163
BsaWI WCCGGW 1 cut(s) 1519
Bsc4I CCNNNNNNNGG 4 cut(s) 407, 1397, 1538, 1680
Bse1I ACTGG 2 cut(s) 523, 998
Bse3DI GCAATG 2 cut(s) 836, 1956
Bse8I GATNNNNATC 2 cut(s) 1117, 1485
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 3 cut(s) 168, 705, 1163
BseGI GGATG 6 cut(s) 226, 604, 669, 869, 1553, 1652
BseJI GATNNNNATC 2 cut(s) 1117, 1485
BseLI CCNNNNNNNGG 4 cut(s) 407, 1397, 1538, 1680
BseMI GCAATG 2 cut(s) 836, 1956
BseMII CTCAG 8 cut(s) 271, 294, 428, 786, 1225, 1230, 1385, 1527
BseNI ACTGG 2 cut(s) 523, 998
BseRI GAGGAG 2 cut(s) 9, 702
BseXI GCAGC 5 cut(s) 436, 1575, 1748, 1766, 1973
BseYI CCCAGC 1 cut(s) 1064
BsgI GTGCAG 2 cut(s) 1001, 1582
BshFI GGCC 4 cut(s) 895, 1509, 1833, 1955
BsiHKAI GWGCWC 1 cut(s) 1212
BsiHKCI CYCGRG 1 cut(s) 684
BsiSI CCGG 1 cut(s) 1520
BslI CCNNNNNNNGG 4 cut(s) 407, 1397, 1538, 1680
BsmAI GTCTC 2 cut(s) 474, 1549
BsmI GAATGC 2 cut(s) 763, 1738
BsnI GGCC 4 cut(s) 895, 1509, 1833, 1955
BsoBI CYCGRG 1 cut(s) 684
Bsp1286I GDGCHC 1 cut(s) 1212
Bsp1720I GCTNAGC 1 cut(s) 1211
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 1 cut(s) 538
BspANI GGCC 4 cut(s) 895, 1509, 1833, 1955
BspCNI CTCAG 8 cut(s) 270, 295, 427, 787, 1224, 1229, 1386, 1528
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 5 cut(s) 18, 658, 894, 1062, 1762
BspPI GGATC 7 cut(s) 224, 651, 664, 1311, 1447, 1523, 1909
BspQI GCTCTTC 2 cut(s) 1267, 1750
BsrDI GCAATG 2 cut(s) 836, 1956
BsrI ACTGG 2 cut(s) 523, 998
BssECI CCNNGG 3 cut(s) 168, 705, 1163
BssT1I CCWWGG 2 cut(s) 168, 1163
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 4 cut(s) 780, 1494, 1795, 1916
Bst6I CTCTTC 5 cut(s) 27, 993, 1267, 1350, 1750
BstAPI GCANNNNNTGC 1 cut(s) 449
BstC8I GCNNGC 3 cut(s) 188, 843, 1741
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 6 cut(s) 226, 604, 669, 869, 1553, 1652
BstMAI GTCTC 2 cut(s) 474, 1549
BstMWI GCNNNNNNNGC 4 cut(s) 272, 449, 901, 1961
BstNI CCWGG 1 cut(s) 707
BstNSI RCATGY 2 cut(s) 337, 771
BstSCI CCNGG 1 cut(s) 705
BstSFI CTRYAG 1 cut(s) 1770
BstV1I GCAGC 5 cut(s) 436, 1575, 1748, 1766, 1973
BstV2I GAAGAC 3 cut(s) 764, 1200, 1329
BstX2I RGATCY 4 cut(s) 656, 734, 1112, 1303
BstYI RGATCY 4 cut(s) 656, 734, 1112, 1303
BsuRI GGCC 4 cut(s) 895, 1509, 1833, 1955
BtgI CCRYGG 1 cut(s) 168
BtgZI GCGATG 1 cut(s) 1093
BtrI CACGTC 3 cut(s) 46, 563, 1671
BtsCI GGATG 6 cut(s) 226, 604, 669, 869, 1553, 1652
BtsIMutI CAGTG 4 cut(s) 90, 265, 1499, 1593
Cac8I GCNNGC 3 cut(s) 188, 843, 1741
CciI TCATGA 1 cut(s) 118
Cfr13I GGNCC 4 cut(s) 893, 1508, 1761, 1798
CseI GACGC 1 cut(s) 1706
Csp6I GTAC 1 cut(s) 1815
CviAII CATG 7 cut(s) 5, 38, 119, 169, 334, 718, 768
CviQI GTAC 1 cut(s) 1815
DraI TTTAAA 2 cut(s) 150, 810
DraIII CACNNNGTG 1 cut(s) 1496
EaeI YGGCCR 2 cut(s) 1831, 1953
Eam1104I CTCTTC 5 cut(s) 27, 993, 1267, 1350, 1750
EarI CTCTTC 5 cut(s) 27, 993, 1267, 1350, 1750
Eco130I CCWWGG 2 cut(s) 168, 1163
Eco47I GGWCC 2 cut(s) 1761, 1798
Eco57I CTGAAG 3 cut(s) 297, 1248, 1776
Eco88I CYCGRG 1 cut(s) 684
EcoRI GAATTC 2 cut(s) 383, 1582
EcoRII CCWGG 1 cut(s) 705
EcoT14I CCWWGG 2 cut(s) 168, 1163
EcoT22I ATGCAT 3 cut(s) 6, 202, 328
ErhI CCWWGG 2 cut(s) 168, 1163
FaeI CATG 7 cut(s) 8, 41, 122, 172, 337, 721, 771
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FatI CATG 7 cut(s) 4, 37, 118, 168, 333, 717, 767
FauNDI CATATG 1 cut(s) 898
FbaI TGATCA 1 cut(s) 1846
Fnu4HI GCNGC 5 cut(s) 450, 1564, 1737, 1780, 1962
FokI GGATG 6 cut(s) 233, 611, 656, 856, 1540, 1659
Fsp4HI GCNGC 5 cut(s) 450, 1564, 1737, 1780, 1962
FspBI CTAG 4 cut(s) 420, 1110, 1164, 1350
GluI GCNGC 5 cut(s) 450, 1564, 1737, 1780, 1962
GsaI CCCAGC 1 cut(s) 1068
GsuI CTGGAG 1 cut(s) 1116
HaeIII GGCC 4 cut(s) 895, 1509, 1833, 1955
HapII CCGG 1 cut(s) 1520
HgaI GACGC 1 cut(s) 1706
Hin1II CATG 7 cut(s) 8, 41, 122, 172, 337, 721, 771
HincII GTYRAC 2 cut(s) 43, 1279
HindII GTYRAC 2 cut(s) 43, 1279
HindIII AAGCTT 1 cut(s) 1607
HinfI GANTC 8 cut(s) 128, 164, 214, 479, 947, 1118, 1825, 1865
HpaI GTTAAC 1 cut(s) 1279
HpaII CCGG 1 cut(s) 1520
HphI GGTGA 4 cut(s) 293, 653, 672, 1673
Hpy166II GTNNAC 4 cut(s) 43, 680, 1279, 1798
Hpy188III TCNNGA 8 cut(s) 119, 161, 218, 794, 1110, 1201, 1627, 1714
Hpy8I GTNNAC 4 cut(s) 43, 680, 1279, 1798
HpyAV CCTTC 8 cut(s) 167, 272, 752, 820, 891, 1499, 1658, 1751
HpyCH4III ACNGT 4 cut(s) 780, 1494, 1795, 1916
HpyCH4IV ACGT 4 cut(s) 45, 562, 833, 1670
HpyF10VI GCNNNNNNNGC 4 cut(s) 272, 449, 901, 1961
HpySE526I ACGT 4 cut(s) 45, 562, 833, 1670
Hsp92II CATG 7 cut(s) 8, 41, 122, 172, 337, 721, 771
Ksp22I TGATCA 1 cut(s) 1846
KspAI GTTAAC 1 cut(s) 1279
LguI GCTCTTC 2 cut(s) 1267, 1750
LmnI GCTCC 4 cut(s) 22, 689, 1655, 1972
Lsp1109I GCAGC 5 cut(s) 436, 1575, 1748, 1766, 1973
LweI GCATC 9 cut(s) 187, 209, 253, 313, 331, 452, 640, 678, 1562
MaeI CTAG 4 cut(s) 420, 1110, 1164, 1350
MaeII ACGT 4 cut(s) 45, 562, 833, 1670
MaeIII GTNAC 4 cut(s) 720, 780, 1588, 1948
MflI RGATCY 4 cut(s) 656, 734, 1112, 1303
MhlI GDGCHC 1 cut(s) 1212
MlsI TGGCCA 1 cut(s) 1833
MluNI TGGCCA 1 cut(s) 1833
MlyI GAGTC 3 cut(s) 223, 941, 1874
MmeI TCCRAC 1 cut(s) 1417
Mox20I TGGCCA 1 cut(s) 1833
Mph1103I ATGCAT 3 cut(s) 6, 202, 328
MscI TGGCCA 1 cut(s) 1833
MseI TTAA 7 cut(s) 149, 210, 347, 545, 809, 971, 1278
MslI CAYNNNNRTG 3 cut(s) 123, 251, 1558
Msp20I TGGCCA 1 cut(s) 1833
MspA1I CMGCKG 2 cut(s) 540, 1535
MspI CCGG 1 cut(s) 1520
MspR9I CCNGG 1 cut(s) 707
Mva1269I GAATGC 2 cut(s) 763, 1738
MvaI CCWGG 1 cut(s) 707
MwoI GCNNNNNNNGC 4 cut(s) 272, 449, 901, 1961
NcoI CCATGG 1 cut(s) 168
NdeI CATATG 1 cut(s) 898
NlaIII CATG 7 cut(s) 8, 41, 122, 172, 337, 721, 771
NlaIV GGNNCC 5 cut(s) 18, 658, 894, 1062, 1762
NmuCI GTSAC 1 cut(s) 1588
NsiI ATGCAT 3 cut(s) 6, 202, 328
NspI RCATGY 2 cut(s) 337, 771
PaeR7I CTCGAG 1 cut(s) 684
PagI TCATGA 1 cut(s) 118
PciSI GCTCTTC 2 cut(s) 1267, 1750
PctI GAATGC 2 cut(s) 763, 1738
PfeI GAWTC 5 cut(s) 128, 164, 479, 1118, 1825
PkrI GCNGC 5 cut(s) 451, 1565, 1738, 1781, 1963
PleI GAGTC 3 cut(s) 222, 941, 1873
PpsI GAGTC 3 cut(s) 222, 941, 1873
Psp6I CCWGG 1 cut(s) 705
PspFI CCCAGC 1 cut(s) 1064
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 5 cut(s) 18, 658, 894, 1062, 1762
PspPI GGNCC 4 cut(s) 893, 1508, 1761, 1798
PspXI VCTCGAGB 1 cut(s) 684
PsuI RGATCY 4 cut(s) 656, 734, 1112, 1303
PvuII CAGCTG 1 cut(s) 1535
RsaI GTAC 1 cut(s) 1816
RsaNI GTAC 1 cut(s) 1815
RseI CAYNNNNRTG 3 cut(s) 123, 251, 1558
SapI GCTCTTC 2 cut(s) 1267, 1750
SaqAI TTAA 7 cut(s) 149, 210, 347, 545, 809, 971, 1278
SatI GCNGC 5 cut(s) 450, 1564, 1737, 1780, 1962
Sau96I GGNCC 4 cut(s) 893, 1508, 1761, 1798
SchI GAGTC 3 cut(s) 223, 941, 1874
ScrFI CCNGG 1 cut(s) 707
SduI GDGCHC 1 cut(s) 1212
SfaNI GCATC 9 cut(s) 187, 209, 253, 313, 331, 452, 640, 678, 1562
SfcI CTRYAG 1 cut(s) 1770
Sfr274I CTCGAG 1 cut(s) 684
SinI GGWCC 2 cut(s) 1761, 1798
SlaI CTCGAG 1 cut(s) 684
SmiMI CAYNNNNRTG 3 cut(s) 123, 251, 1558
SmlI CTYRAG 4 cut(s) 60, 684, 1548, 1967
SmoI CTYRAG 4 cut(s) 60, 684, 1548, 1967
SsiI CCGC 1 cut(s) 538
SspI AATATT 1 cut(s) 802
SspMI CTAG 4 cut(s) 420, 1110, 1164, 1350
StyD4I CCNGG 1 cut(s) 705
StyI CCWWGG 2 cut(s) 168, 1163
TaaI ACNGT 4 cut(s) 780, 1494, 1795, 1916
TaiI ACGT 4 cut(s) 48, 565, 836, 1673
TaqI TCGA 6 cut(s) 131, 685, 945, 1202, 1863, 1885
TfiI GAWTC 5 cut(s) 128, 164, 479, 1118, 1825
Tru1I TTAA 7 cut(s) 149, 210, 347, 545, 809, 971, 1278
Tru9I TTAA 7 cut(s) 149, 210, 347, 545, 809, 971, 1278
TscAI CASTG 4 cut(s) 97, 265, 1499, 1593
TseFI GTSAC 1 cut(s) 1588
TseI GCWGC 5 cut(s) 449, 1563, 1736, 1779, 1961
Tsp45I GTSAC 1 cut(s) 1588
TspGWI ACGGA 1 cut(s) 940
TspRI CASTG 4 cut(s) 97, 265, 1499, 1593
VpaK11BI GGWCC 2 cut(s) 1761, 1798
XapI RAATTY 6 cut(s) 98, 308, 383, 566, 1582, 1622
XbaI TCTAGA 1 cut(s) 1109
XceI RCATGY 2 cut(s) 337, 771
XhoI CTCGAG 1 cut(s) 684
XmaJI CCTAGG 1 cut(s) 1163
XspI CTAG 4 cut(s) 420, 1110, 1164, 1350
Zsp2I ATGCAT 3 cut(s) 6, 202, 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.