MD13G1275100.v1.1

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011552
Physical Location & Seq
Reverse (-)
37595182 .. 37600357
5176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1275100.v1.1.491

Sequence Viewer

Length: 1731 bp
ATGGCAGCAGGAGCCGGCAGTCAGAAAAGCTTAAGAAAAGCGTTGAAAACAGTTGGATTGGCGAAAGCCAATGGTGATTTCAAGGCACTGGACATTGCCATCGCCAAGGCCACGAATCATGATGAACGACTGCCCAAGGAGAAACATGTTATAACTATATTAAATGCAGTGTCAGCTTCAAGACCTCGTGCTGAAGTTGCGTATTGCATCTTTGGTCTTGCCAGGCGTCTTTCTAAGACCCACAGTTGGAAGGTAGCACTGAAAACTGTGATTGTTGTGCACCGTGGGTTGAGGGAAGTTGATGATACATTTTGCGACTCGCTAGTCAAATATAGCTGGAGTAGAGGTCATATACTGGATTTATCTCATGTTTGGGATCAATCAAGTACAAGTGCATTGGGTTATTCTGCCTGGGTGCGGACATATGCATCATATCTTGAAGAGCACCTTGAATGTTTCCGGGTATTGAAGTATGACGTTCAGAAAGATCACTCGAAAACCATGGAACTGGATACCACAGACTTACTAAGGCAATTACCCGCTTTGCAACAGCTTCTTTCTCGCCTTCTTGATTGCCAGCCAGAGGTGGGAACACTATATAATGTCTTGATTCAATATGCCCTTTCAATGGTTGCAGGTGAAAGTGCAAAGCTGTATGCTGCAATTAATGGTGGTATTGTCAATTTACTTGACAAGTTTTTTGAGATGCAGCGAAATGATGCAGTTAAAGCACTTGAAATATATAAGAAGTCAGGAAGTCAGGAAGAGAGGTTATCGGAGTTCTTTGAGAGCTGCAGGAGCCTTAATTTTGGACGGGCACTGAAATTCATTAAAATTGAACGGCCCCCTGCATCATTCTTGACTACCATGGAAGATTATGTGAAGGAGGCTCCCTGCAGTACAAAGCTTCAATGTACACCGACCTATGATGAGCAAGATTCTGCTCCCAAGGAAGTTGAGACGATAGAAGGTGATTTCTTAATTGACCATGAACCAGATGATAATAATAGTGAACAAAAATCAAATGAAGGCCCAAAGAGTGAGAGTGAGGCTGCTGCCACATCACATGTTGTTGACCTCAGCTTCGATGAATTAACTCTGGCAACATCAGAATCGGATGAGAAAAATTCCCTCGCACTTGCAATCGTTGAGCCTGATTCCAAACAAGAATGTCTGAATACGACAAGTGCGGAGGAGTCCAGTTGGGAGGTGGCACTTTTTACAGCACCAAGTTGCCATGCAGCTGCAGCTACAGCACCAACTTCTAATGTAGCTCCGGTTGAAGAGACAAAGTCGGGTGTTGGGCTGGACAGGTTGACACTGGACAGTCTGTACGATGGTGTAATCACAAGTACACAAAATCAGAATAATAACGCGATATACTACCATAGACAGATGCCGTCTAATCCTTTTGATGATGTTGTTGATCCTTACCAATTAGCCTCCCCATTTCACCCAACCAACATGCAGATGGCTATCATGCCCCAACAAGTACAACAAGGCCAAGTCCCATTTTATGCTTCGTATAGCAATGCACTTGCAATACCGCCAACCAACGTGCAAATGCCTCCTTTCATGCATCCACAACAAGTTTTCATTATGCAGCAGCAGCAACAGCAAGTGCAACAGCAGCAGTTAACCAACATCAGCCATAACCCTACAAATTCTTCCAGCAATCCCTTCGCTATTGATCAGAGTTATTCACAACCACCTTCAGAACTCTTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

577

Amino Acids

64.07

Weight (kDa)

5.15

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 30 - 295 8.9e-72 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AarI CACCTGC 1 cut(s) 626
AasI GACNNNNNNGTC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 626
AccII CGCG 1 cut(s) 1376
AciI CCGC 4 cut(s) 418, 540, 1190, 1547
AclWI GGATC 2 cut(s) 384, 1421
AcsI RAATTY 3 cut(s) 824, 1126, 1663
AcuI CTGAAG 2 cut(s) 213, 1698
AcyI GRCGYC 1 cut(s) 226
AfaI GTAC 6 cut(s) 388, 901, 916, 1334, 1354, 1494
AfiI CCNNNNNNNGG 5 cut(s) 246, 417, 583, 587, 628
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 2 cut(s) 145, 1066
AjnI CCWGG 2 cut(s) 221, 410
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
Alw21I GWGCWC 2 cut(s) 282, 447
Alw26I GTCTC 2 cut(s) 953, 1280
Alw44I GTGCAC 1 cut(s) 278
AlwI GGATC 2 cut(s) 384, 1421
AoxI GGCC 4 cut(s) 108, 842, 1030, 1501
ApaLI GTGCAC 1 cut(s) 278
ApoI RAATTY 3 cut(s) 824, 1126, 1663
ArsI GACNNNNNNTTYG 4 cut(s) 683, 715, 1067, 1099
AseI ATTAAT 1 cut(s) 666
AspS9I GGNCC 2 cut(s) 843, 1031
AsuC2I CCSGG 1 cut(s) 461
AsuHPI GGTGA 4 cut(s) 86, 650, 983, 1445
BaeGI GKGCMC 2 cut(s) 282, 820
BauI CACGAG 1 cut(s) 186
Bbv12I GWGCWC 2 cut(s) 282, 447
BbvCI CCTCAGC 1 cut(s) 1079
BccI CCATC 3 cut(s) 107, 1331, 1465
BceAI ACGGC 2 cut(s) 857, 1384
BcgI CGANNNNNNTGC 2 cut(s) 1663, 1697
BciT130I CCWGG 2 cut(s) 223, 412
BciVI GTATCC 1 cut(s) 505
BclI TGATCA 1 cut(s) 1690
BcnI CCSGG 1 cut(s) 461
BcoDI GTCTC 2 cut(s) 953, 1280
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 4 cut(s) 793, 895, 1245, 1251
BfrI CTTAAG 1 cut(s) 31
BfuAI ACCTGC 1 cut(s) 626
BfuI GTATCC 1 cut(s) 505
Bme1390I CCNGG 3 cut(s) 223, 412, 461
BmgT120I GGNCC 2 cut(s) 843, 1031
BmiI GGNNCC 4 cut(s) 13, 800, 845, 891
BmrFI CCNGG 3 cut(s) 223, 412, 461
BmsI GCATC 7 cut(s) 216, 437, 696, 709, 860, 1386, 1588
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 1079
BpuMI CCSGG 1 cut(s) 461
BsaBI GATNNNNATC 1 cut(s) 1475
BsaHI GRCGYC 1 cut(s) 226
BsaJI CCNNGG 7 cut(s) 105, 135, 283, 411, 501, 867, 948
BsaWI WCCGGW 1 cut(s) 1276
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 5 cut(s) 246, 417, 583, 587, 628
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 5 cut(s) 93, 360, 513, 1200, 1326
Bse3DI GCAATG 2 cut(s) 93, 1537
Bse8I GATNNNNATC 1 cut(s) 1475
BseBI CCWGG 2 cut(s) 223, 412
BseDI CCNNGG 7 cut(s) 105, 135, 283, 411, 501, 867, 948
BseGI GGATG 2 cut(s) 1123, 1579
BseJI GATNNNNATC 1 cut(s) 1475
BseLI CCNNNNNNNGG 5 cut(s) 246, 417, 583, 587, 628
BseMI GCAATG 2 cut(s) 93, 1537
BseMII CTCAG 1 cut(s) 1093
BseNI ACTGG 5 cut(s) 93, 360, 513, 1200, 1326
BseRI GAGGAG 1 cut(s) 1208
BseSI GKGCMC 2 cut(s) 282, 820
Bsh1236I CGCG 1 cut(s) 1376
BshFI GGCC 4 cut(s) 110, 844, 1032, 1503
BsiHKAI GWGCWC 2 cut(s) 282, 447
BsiSI CCGG 3 cut(s) 15, 460, 1277
BslFI GGGAC 1 cut(s) 1493
BslI CCNNNNNNNGG 5 cut(s) 246, 417, 583, 587, 628
BsmAI GTCTC 2 cut(s) 953, 1280
BsmBI CGTCTC 1 cut(s) 953
BsmFI GGGAC 1 cut(s) 1493
BsnI GGCC 4 cut(s) 110, 844, 1032, 1503
Bsp1286I GDGCHC 3 cut(s) 282, 447, 820
Bsp1407I TGTACA 1 cut(s) 914
Bsp143I GATC 4 cut(s) 376, 487, 1426, 1690
Bsp19I CCATGG 2 cut(s) 501, 867
BspACI CCGC 4 cut(s) 418, 540, 1190, 1547
BspANI GGCC 4 cut(s) 110, 844, 1032, 1503
BspCNI CTCAG 1 cut(s) 1092
BspFNI CGCG 1 cut(s) 1376
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 4 cut(s) 13, 800, 845, 891
BspMAI CTGCAG 3 cut(s) 797, 899, 1249
BspMI ACCTGC 1 cut(s) 626
BspPI GGATC 2 cut(s) 384, 1421
BspQI GCTCTTC 1 cut(s) 435
BspTI CTTAAG 1 cut(s) 31
BsrDI GCAATG 2 cut(s) 93, 1537
BsrFI RCCGGY 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 914
BsrI ACTGG 5 cut(s) 93, 360, 513, 1200, 1326
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 7 cut(s) 105, 135, 283, 411, 501, 867, 948
BssMI GATC 4 cut(s) 376, 487, 1426, 1690
BssNI GRCGYC 1 cut(s) 226
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 5 cut(s) 105, 135, 501, 867, 948
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 223, 412
Bst4CI ACNGT 5 cut(s) 52, 245, 268, 284, 1328
Bst6I CTCTTC 3 cut(s) 435, 759, 1278
BstACI GRCGYC 1 cut(s) 226
BstAFI CTTAAG 1 cut(s) 31
BstAUI TGTACA 1 cut(s) 914
BstC8I GCNNGC 2 cut(s) 16, 578
BstDEI CTNAG 3 cut(s) 234, 527, 1079
BstDSI CCRYGG 3 cut(s) 283, 501, 867
BstF5I GGATG 2 cut(s) 1123, 1579
BstFNI CGCG 1 cut(s) 1376
BstKTI GATC 4 cut(s) 379, 490, 1429, 1693
BstMAI GTCTC 2 cut(s) 953, 1280
BstMBI GATC 4 cut(s) 376, 487, 1426, 1690
BstNI CCWGG 2 cut(s) 223, 412
BstNSI RCATGY 3 cut(s) 149, 1070, 1468
BstSCI CCNGG 3 cut(s) 221, 410, 459
BstSFI CTRYAG 4 cut(s) 793, 895, 1245, 1251
BstSLI GKGCMC 2 cut(s) 282, 820
BstUI CGCG 1 cut(s) 1376
BstXI CCANNNNNNTGG 1 cut(s) 508
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 4 cut(s) 110, 844, 1032, 1503
BtgI CCRYGG 3 cut(s) 283, 501, 867
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 2 cut(s) 1123, 1579
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 5 cut(s) 86, 174, 257, 818, 1319
BveI ACCTGC 1 cut(s) 626
Cac8I GCNNGC 2 cut(s) 16, 578
CciI TCATGA 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 843, 1031
CseI GACGC 1 cut(s) 215
Csp6I GTAC 6 cut(s) 387, 900, 915, 1333, 1353, 1493
CviQI GTAC 6 cut(s) 387, 900, 915, 1333, 1353, 1493
DdeI CTNAG 3 cut(s) 234, 527, 1079
DpnI GATC 4 cut(s) 378, 489, 1428, 1692
DpnII GATC 4 cut(s) 376, 487, 1426, 1690
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
Eam1104I CTCTTC 3 cut(s) 435, 759, 1278
EarI CTCTTC 3 cut(s) 435, 759, 1278
Eco130I CCWWGG 5 cut(s) 105, 135, 501, 867, 948
Eco57I CTGAAG 2 cut(s) 213, 1698
EcoRII CCWGG 2 cut(s) 221, 410
EcoT14I CCWWGG 5 cut(s) 105, 135, 501, 867, 948
EcoT22I ATGCAT 2 cut(s) 430, 1581
ErhI CCWWGG 5 cut(s) 105, 135, 501, 867, 948
Esp3I CGTCTC 1 cut(s) 953
FalI AAGNNNNNCTT 2 cut(s) 432, 464
FaqI GGGAC 1 cut(s) 1493
FauI CCCGC 1 cut(s) 547
FauNDI CATATG 1 cut(s) 424
FbaI TGATCA 1 cut(s) 1690
FokI GGATG 2 cut(s) 1130, 1566
FspBI CTAG 1 cut(s) 323
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 4 cut(s) 110, 844, 1032, 1503
HapII CCGG 3 cut(s) 15, 460, 1277
HgaI GACGC 1 cut(s) 215
Hin1I GRCGYC 1 cut(s) 226
HincII GTYRAC 3 cut(s) 1075, 1317, 1638
HindII GTYRAC 3 cut(s) 1075, 1317, 1638
HindIII AAGCTT 2 cut(s) 28, 905
HinfI GANTC 7 cut(s) 115, 317, 610, 938, 1112, 1157, 1196
HpaI GTTAAC 1 cut(s) 1638
HpaII CCGG 3 cut(s) 15, 460, 1277
HphI GGTGA 4 cut(s) 86, 650, 983, 1445
Hpy166II GTNNAC 7 cut(s) 280, 917, 1013, 1075, 1317, 1355, 1638
Hpy188I TCNGA 9 cut(s) 24, 483, 778, 1111, 1117, 1176, 1365, 1695, 1717
Hpy188III TCNNGA 8 cut(s) 119, 180, 437, 569, 607, 753, 761, 859
Hpy8I GTNNAC 7 cut(s) 280, 917, 1013, 1075, 1317, 1355, 1638
HpyAV CCTTC 7 cut(s) 244, 575, 877, 962, 1022, 1690, 1722
HpyCH4III ACNGT 5 cut(s) 52, 245, 268, 284, 1328
HpyCH4IV ACGT 2 cut(s) 477, 1557
HpyF3I CTNAG 3 cut(s) 234, 527, 1079
HpySE526I ACGT 2 cut(s) 477, 1557
Hsp92I GRCGYC 1 cut(s) 226
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 1690
KspAI GTTAAC 1 cut(s) 1638
Kzo9I GATC 4 cut(s) 376, 487, 1426, 1690
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 5 cut(s) 11, 798, 895, 949, 1279
LweI GCATC 7 cut(s) 216, 437, 696, 709, 860, 1386, 1588
MaeI CTAG 1 cut(s) 323
MaeII ACGT 2 cut(s) 477, 1557
MalI GATC 4 cut(s) 378, 489, 1428, 1692
MboI GATC 4 cut(s) 376, 487, 1426, 1690
MboII GAAGA 5 cut(s) 452, 776, 884, 1295, 1659
MhlI GDGCHC 3 cut(s) 282, 447, 820
MlyI GAGTC 2 cut(s) 311, 1205
MmeI TCCRAC 2 cut(s) 34, 227
Mph1103I ATGCAT 2 cut(s) 430, 1581
MroNI GCCGGC 1 cut(s) 14
MseI TTAA 9 cut(s) 32, 161, 666, 726, 804, 831, 980, 1094, 1637
MslI CAYNNNNRTG 1 cut(s) 1469
MspA1I CMGCKG 1 cut(s) 1244
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 3 cut(s) 15, 460, 1277
MspR9I CCNGG 3 cut(s) 223, 412, 461
MvaI CCWGG 2 cut(s) 223, 412
MvnI CGCG 1 cut(s) 1376
NaeI GCCGGC 1 cut(s) 16
NciI CCSGG 1 cut(s) 461
NcoI CCATGG 2 cut(s) 501, 867
NdeI CATATG 1 cut(s) 424
NdeII GATC 4 cut(s) 376, 487, 1426, 1690
NgoMIV GCCGGC 1 cut(s) 14
NlaIV GGNNCC 4 cut(s) 13, 800, 845, 891
NsiI ATGCAT 2 cut(s) 430, 1581
NspI RCATGY 3 cut(s) 149, 1070, 1468
PagI TCATGA 1 cut(s) 118
PaqCI CACCTGC 1 cut(s) 626
PciI ACATGT 2 cut(s) 145, 1066
PciSI GCTCTTC 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 16
PfeI GAWTC 5 cut(s) 115, 610, 938, 1112, 1157
PflFI GACNNNGTC 1 cut(s) 1291
PleI GAGTC 2 cut(s) 311, 1204
PpsI GAGTC 2 cut(s) 311, 1204
PscI ACATGT 2 cut(s) 145, 1066
PshBI ATTAAT 1 cut(s) 666
PsiI TTATAA 1 cut(s) 152
Psp6I CCWGG 2 cut(s) 221, 410
PspGI CCWGG 2 cut(s) 221, 410
PspN4I GGNNCC 4 cut(s) 13, 800, 845, 891
PspPI GGNCC 2 cut(s) 843, 1031
PstI CTGCAG 3 cut(s) 797, 899, 1249
PsyI GACNNNGTC 1 cut(s) 1291
PvuII CAGCTG 1 cut(s) 1244
RsaI GTAC 6 cut(s) 388, 901, 916, 1334, 1354, 1494
RsaNI GTAC 6 cut(s) 387, 900, 915, 1333, 1353, 1493
RseI CAYNNNNRTG 1 cut(s) 1469
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 9 cut(s) 32, 161, 666, 726, 804, 831, 980, 1094, 1637
Sau3AI GATC 4 cut(s) 376, 487, 1426, 1690
Sau96I GGNCC 2 cut(s) 843, 1031
SchI GAGTC 2 cut(s) 311, 1205
ScrFI CCNGG 3 cut(s) 223, 412, 461
SduI GDGCHC 3 cut(s) 282, 447, 820
SfaNI GCATC 7 cut(s) 216, 437, 696, 709, 860, 1386, 1588
SfcI CTRYAG 4 cut(s) 793, 895, 1245, 1251
SmiMI CAYNNNNRTG 1 cut(s) 1469
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
SsiI CCGC 4 cut(s) 418, 540, 1190, 1547
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 3 cut(s) 221, 410, 459
StyI CCWWGG 5 cut(s) 105, 135, 501, 867, 948
TaaI ACNGT 5 cut(s) 52, 245, 268, 284, 1328
TaiI ACGT 2 cut(s) 480, 1560
TaqI TCGA 2 cut(s) 494, 1086
TatI WGTACW 5 cut(s) 386, 899, 914, 1352, 1492
TfiI GAWTC 5 cut(s) 115, 610, 938, 1112, 1157
Tru1I TTAA 9 cut(s) 32, 161, 666, 726, 804, 831, 980, 1094, 1637
Tru9I TTAA 9 cut(s) 32, 161, 666, 726, 804, 831, 980, 1094, 1637
TscAI CASTG 5 cut(s) 93, 174, 264, 825, 1326
TspDTI ATGAA 7 cut(s) 138, 817, 1005, 1041, 1104, 1564, 1585
TspRI CASTG 5 cut(s) 93, 174, 264, 825, 1326
Tth111I GACNNNGTC 1 cut(s) 1291
Vha464I CTTAAG 1 cut(s) 31
VneI GTGCAC 1 cut(s) 278
VspI ATTAAT 1 cut(s) 666
XapI RAATTY 3 cut(s) 824, 1126, 1663
XceI RCATGY 3 cut(s) 149, 1070, 1468
XcmI CCANNNNNNNNNTGG 2 cut(s) 1207, 1468
XspI CTAG 1 cut(s) 323
Zsp2I ATGCAT 2 cut(s) 430, 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.