pycom13g28660

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011552
Physical Location & Seq
Forward (+)
24615303 .. 24619727
4425 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g28660.6

Sequence Viewer

Length: 1764 bp
ATGGCAGCAGGCGCCGGCAGTCAGAAAAGCTTAAGAAAAGCGTTGAAAACAGTTGGATTGGCGAAAGCCAATGGTGATTTCAAGGCACTGGACATTGCCATCGCCAAGGCCACGAATCATGATGAACTACTGCCCAAGGAGAAACATGTTATAACTATATTAAATGCAGTGTCAGCTTCAAGACCTCGCGCTGAAGTTGCGTATTGCATCTTTGGTCTTGCCAGGCGTCTTTCTAAGACCCACAGTTGGAAGGTAGCACTGAAAACTGTGATTGTTGTGCACCGCGGGTTGAGGGAAGTTGATGATACATTTTGCGACTCGCTAGTCAAATATAGCTGGAGTAGAGGTCATATGCTGGATTTATCTCATGTTTGGGATGAATCAAGTACAAGTGCATTGGGTTATTCTGCCTGGGTTCGGACATATGCATCATATCTTGAAGAGCACCTTGAATGTTTCCGCGTATTGAAGTATGACGTTCAGAAAGATCACTCGAAACCCAAGGAACTTGATACCACAGACTTACTAAGGCAATTACCCGCTTTGCAACACCTTCTTTCTCGCCTTCTTGATTGCCAGCCAGAGGTGGGAACACTATATAATGTCTTGATTCAATATGCCCTTTCAATGGTTGCAGGTGAAAGTGCAAAGCTGTATGCTGCAATTAATGGTGGTATTGTCAATTTACTTGACAAGGTTTTTGAGATGCAGCGAGATGATGCAGTTAAAGCACTTGAAATATATAAGAAGTCAGGAAGTCAGGAAGAGAGGTTATCGGAGTTCTTTGAGAGCTGCAGGAGCCTTAATTTTGGACGGGCACTGAAATTCATTAAAATTGAACGGGCCCCTGCATCATTCTTGACTACCCTGGAAGATTATGTGAAGGAGGCTCCCTGCAGTACAACGCTTCAATGTACACCGACCGATGATGAGCAAGATTCTGCTCCCAAGGAAGTTGAGACGATAGAAGGTGATTTGTTAATTGACCACAAACCAGATGATAATAATAGTGAACAAAAATCAAATGAAGGCCCAAAGAGTGAGAGTGAGGCTGCTGCCACATCACATGTTGTTGACCTCCTGAGCTTCGATGATTTAACTCTGGCAACATCAGAATCGGATGAGAAAAATTCCCTCGCACTTGCAATCGTTGAGCCTGATTCCAAACCAGAATGTCTGAATACGACAAGTGCGGAGGAGTCCAGTTGGGAGGTGGCACTTTTTACAGCACCAAGTTGCCACGCAGCTGCAGTTACAGCACCAACTTCTAATGTAGCTCCAGTTGAAGAGATAAAGTCGGGTGTTGGGCTGGACAGGTTGACACTGGACAGTCTGTACGATGGTGCAATCACAAGTACACAAAATCAGAATAATAACGTGATGTACTACCATAGACAGATGCCGTCTAATCCTTTCGATGATGTTGTTGATCCTTACCAATTAGCCTCCCCATTTCACCCAACCAACATGCAGATGGCTATCATGCCCCAACAAGTACAACAAGGCCAAGTACCATTTTATGCTTCGTATAGCAATGCACTTGCAATACCGCCAACCAACATGCAAATGCCTCCTTTCATGCCTCAACAACAAGCTTTCATTATGCAGCAGCAGCAACAGCAGCAGTTAGCCAACATCAGCCATAACCCCACAAATTCTTCCAGCAATCCCTTCGCTATTGATCAGAGTTATTCACAACCACCTTCAGAATTCTTGCAATGGCATGGTTTAGTTGATCCCCCAAAAGAAGTTGAGCATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

588

Amino Acids

65.16

Weight (kDa)

5.07

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AarI CACCTGC 1 cut(s) 626
AasI GACNNNNNNGTC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 626
AccB1I GGYRCC 1 cut(s) 11
AccII CGCG 3 cut(s) 189, 285, 462
AciI CCGC 6 cut(s) 283, 285, 460, 540, 1193, 1550
AclWI GGATC 2 cut(s) 1424, 1730
AcsI RAATTY 4 cut(s) 824, 1129, 1654, 1709
AcuI CTGAAG 2 cut(s) 213, 1689
AcyI GRCGYC 2 cut(s) 12, 226
AfaI GTAC 8 cut(s) 388, 901, 916, 1337, 1357, 1385, 1497, 1512
AfiI CCNNNNNNNGG 5 cut(s) 246, 417, 583, 587, 628
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 2 cut(s) 145, 1066
AjnI CCWGG 3 cut(s) 221, 410, 867
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 9 cut(s) 30, 176, 336, 652, 792, 1086, 1247, 1277, 1595
AluI AGCT 9 cut(s) 30, 176, 336, 652, 792, 1086, 1247, 1277, 1595
Alw21I GWGCWC 2 cut(s) 282, 447
Alw26I GTCTC 1 cut(s) 953
Alw44I GTGCAC 1 cut(s) 278
AlwI GGATC 2 cut(s) 1424, 1730
AoxI GGCC 4 cut(s) 108, 843, 1030, 1504
ApaI GGGCCC 1 cut(s) 847
ApaLI GTGCAC 1 cut(s) 278
ApoI RAATTY 4 cut(s) 824, 1129, 1654, 1709
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
AseI ATTAAT 1 cut(s) 666
AspLEI GCGC 2 cut(s) 14, 191
AspS9I GGNCC 3 cut(s) 843, 844, 1031
AsuHPI GGTGA 4 cut(s) 86, 650, 983, 1448
BaeGI GKGCMC 3 cut(s) 282, 820, 847
BanI GGYRCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 847
Bbv12I GWGCWC 2 cut(s) 282, 447
BccI CCATC 3 cut(s) 107, 1334, 1468
BceAI ACGGC 1 cut(s) 1387
BcgI CGANNNNNNTGC 2 cut(s) 1654, 1688
BciT130I CCWGG 3 cut(s) 223, 412, 869
BclI TGATCA 1 cut(s) 1681
BcoDI GTCTC 1 cut(s) 953
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 3 cut(s) 793, 895, 1248
BfoI RGCGCY 1 cut(s) 15
BfrI CTTAAG 1 cut(s) 31
BfuAI ACCTGC 1 cut(s) 626
Bme1390I CCNGG 3 cut(s) 223, 412, 869
BmgT120I GGNCC 3 cut(s) 843, 844, 1031
BmiI GGNNCC 5 cut(s) 13, 800, 845, 846, 891
BmrFI CCNGG 3 cut(s) 223, 412, 869
BmsI GCATC 6 cut(s) 216, 437, 696, 709, 860, 1389
BpmI CTGGAG 2 cut(s) 358, 1263
Bpu10I CCTNAGC 1 cut(s) 1082
BsaBI GATNNNNATC 1 cut(s) 1478
BsaHI GRCGYC 2 cut(s) 12, 226
BsaJI CCNNGG 7 cut(s) 105, 135, 283, 411, 501, 867, 948
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 5 cut(s) 246, 417, 583, 587, 628
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 4 cut(s) 93, 1203, 1280, 1329
Bse3DI GCAATG 3 cut(s) 93, 1540, 1724
Bse8I GATNNNNATC 1 cut(s) 1478
BseBI CCWGG 3 cut(s) 223, 412, 869
BseDI CCNNGG 7 cut(s) 105, 135, 283, 411, 501, 867, 948
BseGI GGATG 2 cut(s) 382, 1126
BseJI GATNNNNATC 1 cut(s) 1478
BseLI CCNNNNNNNGG 5 cut(s) 246, 417, 583, 587, 628
BseMI GCAATG 3 cut(s) 93, 1540, 1724
BseMII CTCAG 1 cut(s) 1073
BseNI ACTGG 4 cut(s) 93, 1203, 1280, 1329
BseRI GAGGAG 1 cut(s) 1211
BseSI GKGCMC 3 cut(s) 282, 820, 847
Bsh1236I CGCG 3 cut(s) 189, 285, 462
Bsh1285I CGRYCG 1 cut(s) 924
BshFI GGCC 4 cut(s) 110, 845, 1032, 1506
BshNI GGYRCC 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 924
BsiHKAI GWGCWC 2 cut(s) 282, 447
BsiSI CCGG 1 cut(s) 15
BslI CCNNNNNNNGG 5 cut(s) 246, 417, 583, 587, 628
BsmAI GTCTC 1 cut(s) 953
BsmBI CGTCTC 1 cut(s) 953
BsnI GGCC 4 cut(s) 110, 845, 1032, 1506
Bsp120I GGGCCC 1 cut(s) 843
Bsp1286I GDGCHC 4 cut(s) 282, 447, 820, 847
Bsp1407I TGTACA 1 cut(s) 914
Bsp143I GATC 4 cut(s) 487, 1429, 1681, 1735
BspACI CCGC 6 cut(s) 283, 285, 460, 540, 1193, 1550
BspANI GGCC 4 cut(s) 110, 845, 1032, 1506
BspCNI CTCAG 1 cut(s) 1074
BspFNI CGCG 3 cut(s) 189, 285, 462
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 5 cut(s) 13, 800, 845, 846, 891
BspMAI CTGCAG 3 cut(s) 797, 899, 1252
BspMI ACCTGC 1 cut(s) 626
BspPI GGATC 2 cut(s) 1424, 1730
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 11
BspTI CTTAAG 1 cut(s) 31
BsrDI GCAATG 3 cut(s) 93, 1540, 1724
BsrFI RCCGGY 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 914
BsrI ACTGG 4 cut(s) 93, 1203, 1280, 1329
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 7 cut(s) 105, 135, 283, 411, 501, 867, 948
BssMI GATC 4 cut(s) 487, 1429, 1681, 1735
BssNI GRCGYC 2 cut(s) 12, 226
BssT1I CCWWGG 4 cut(s) 105, 135, 501, 948
Bst2UI CCWGG 3 cut(s) 223, 412, 869
Bst4CI ACNGT 4 cut(s) 52, 245, 268, 1331
Bst6I CTCTTC 3 cut(s) 435, 759, 1281
BstACI GRCGYC 2 cut(s) 12, 226
BstAFI CTTAAG 1 cut(s) 31
BstAUI TGTACA 1 cut(s) 914
BstC8I GCNNGC 3 cut(s) 10, 16, 578
BstDEI CTNAG 3 cut(s) 234, 527, 1082
BstDSI CCRYGG 1 cut(s) 283
BstF5I GGATG 2 cut(s) 382, 1126
BstFNI CGCG 3 cut(s) 189, 285, 462
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 191
BstKTI GATC 4 cut(s) 490, 1432, 1684, 1738
BstMAI GTCTC 1 cut(s) 953
BstMBI GATC 4 cut(s) 487, 1429, 1681, 1735
BstMCI CGRYCG 1 cut(s) 924
BstMWI GCNNNNNNNGC 9 cut(s) 11, 173, 197, 728, 798, 1256, 1612, 1618, 1621
BstNI CCWGG 3 cut(s) 223, 412, 869
BstNSI RCATGY 4 cut(s) 149, 1070, 1471, 1564
BstSCI CCNGG 3 cut(s) 221, 410, 867
BstSFI CTRYAG 3 cut(s) 793, 895, 1248
BstSLI GKGCMC 3 cut(s) 282, 820, 847
BstUI CGCG 3 cut(s) 189, 285, 462
BsuRI GGCC 4 cut(s) 110, 845, 1032, 1506
BtgI CCRYGG 1 cut(s) 283
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 2 cut(s) 382, 1126
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 5 cut(s) 86, 174, 257, 818, 1322
BveI ACCTGC 1 cut(s) 626
Cac8I GCNNGC 3 cut(s) 10, 16, 578
CciI TCATGA 1 cut(s) 118
CfoI GCGC 2 cut(s) 14, 191
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 3 cut(s) 843, 844, 1031
Cfr42I CCGCGG 1 cut(s) 286
CseI GACGC 1 cut(s) 215
Csp6I GTAC 8 cut(s) 387, 900, 915, 1336, 1356, 1384, 1496, 1511
CviAII CATG 9 cut(s) 119, 146, 368, 1067, 1468, 1483, 1561, 1579, 1724
CviQI GTAC 8 cut(s) 387, 900, 915, 1336, 1356, 1384, 1496, 1511
DdeI CTNAG 3 cut(s) 234, 527, 1082
DinI GGCGCC 1 cut(s) 13
DpnI GATC 4 cut(s) 489, 1431, 1683, 1737
DpnII GATC 4 cut(s) 487, 1429, 1681, 1735
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
Eam1104I CTCTTC 3 cut(s) 435, 759, 1281
EarI CTCTTC 3 cut(s) 435, 759, 1281
Eco130I CCWWGG 4 cut(s) 105, 135, 501, 948
Eco24I GRGCYC 1 cut(s) 847
Eco57I CTGAAG 2 cut(s) 213, 1689
EcoO109I RGGNCCY 1 cut(s) 844
EcoRI GAATTC 1 cut(s) 1709
EcoRII CCWGG 3 cut(s) 221, 410, 867
EcoT14I CCWWGG 4 cut(s) 105, 135, 501, 948
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 847
EgeI GGCGCC 1 cut(s) 13
EheI GGCGCC 1 cut(s) 13
ErhI CCWWGG 4 cut(s) 105, 135, 501, 948
Esp3I CGTCTC 1 cut(s) 953
FaeI CATG 9 cut(s) 122, 149, 371, 1070, 1471, 1486, 1564, 1582, 1727
FalI AAGNNNNNCTT 2 cut(s) 432, 464
FatI CATG 9 cut(s) 118, 145, 367, 1066, 1467, 1482, 1560, 1578, 1723
FauI CCCGC 2 cut(s) 278, 547
FauNDI CATATG 2 cut(s) 351, 424
FbaI TGATCA 1 cut(s) 1681
FokI GGATG 2 cut(s) 389, 1133
FriOI GRGCYC 1 cut(s) 847
FspBI CTAG 1 cut(s) 323
GlaI GCGC 2 cut(s) 13, 190
GsuI CTGGAG 2 cut(s) 358, 1263
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 4 cut(s) 110, 845, 1032, 1506
HapII CCGG 1 cut(s) 15
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 14, 191
Hin1I GRCGYC 2 cut(s) 12, 226
Hin1II CATG 9 cut(s) 122, 149, 371, 1070, 1471, 1486, 1564, 1582, 1727
Hin6I GCGC 2 cut(s) 12, 189
HinP1I GCGC 2 cut(s) 12, 189
HincII GTYRAC 2 cut(s) 1075, 1320
HindII GTYRAC 2 cut(s) 1075, 1320
HindIII AAGCTT 2 cut(s) 28, 1593
HinfI GANTC 8 cut(s) 115, 317, 380, 610, 938, 1115, 1160, 1199
HpaII CCGG 1 cut(s) 15
HphI GGTGA 4 cut(s) 86, 650, 983, 1448
Hpy166II GTNNAC 6 cut(s) 280, 917, 1013, 1075, 1320, 1358
Hpy188III TCNNGA 9 cut(s) 119, 180, 437, 569, 607, 753, 761, 859, 1081
Hpy8I GTNNAC 6 cut(s) 280, 917, 1013, 1075, 1320, 1358
HpyAV CCTTC 8 cut(s) 244, 563, 575, 877, 962, 1022, 1681, 1713
HpyCH4III ACNGT 4 cut(s) 52, 245, 268, 1331
HpyCH4IV ACGT 2 cut(s) 477, 1377
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 173, 197, 728, 798, 1256, 1612, 1618, 1621
HpyF3I CTNAG 3 cut(s) 234, 527, 1082
HpySE526I ACGT 2 cut(s) 477, 1377
Hsp92I GRCGYC 2 cut(s) 12, 226
Hsp92II CATG 9 cut(s) 122, 149, 371, 1070, 1471, 1486, 1564, 1582, 1727
HspAI GCGC 2 cut(s) 12, 189
KasI GGCGCC 1 cut(s) 11
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 1681
KspI CCGCGG 1 cut(s) 286
Kzo9I GATC 4 cut(s) 487, 1429, 1681, 1735
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 4 cut(s) 798, 895, 949, 1282
LweI GCATC 6 cut(s) 216, 437, 696, 709, 860, 1389
MaeI CTAG 1 cut(s) 323
MaeII ACGT 2 cut(s) 477, 1377
MaeIII GTNAC 1 cut(s) 1252
MalI GATC 4 cut(s) 489, 1431, 1683, 1737
MboI GATC 4 cut(s) 487, 1429, 1681, 1735
MboII GAAGA 5 cut(s) 452, 776, 884, 1298, 1650
MhlI GDGCHC 4 cut(s) 282, 447, 820, 847
Mly113I GGCGCC 1 cut(s) 12
MlyI GAGTC 2 cut(s) 311, 1208
MmeI TCCRAC 2 cut(s) 34, 227
Mph1103I ATGCAT 1 cut(s) 430
MroNI GCCGGC 1 cut(s) 14
MseI TTAA 8 cut(s) 32, 161, 666, 726, 804, 831, 980, 1097
MslI CAYNNNNRTG 2 cut(s) 1472, 1565
MspA1I CMGCKG 2 cut(s) 285, 1247
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 3 cut(s) 223, 412, 869
MvaI CCWGG 3 cut(s) 223, 412, 869
MvnI CGCG 3 cut(s) 189, 285, 462
MwoI GCNNNNNNNGC 9 cut(s) 11, 173, 197, 728, 798, 1256, 1612, 1618, 1621
NaeI GCCGGC 1 cut(s) 16
NarI GGCGCC 1 cut(s) 12
NdeI CATATG 2 cut(s) 351, 424
NdeII GATC 4 cut(s) 487, 1429, 1681, 1735
NgoMIV GCCGGC 1 cut(s) 14
NlaIII CATG 9 cut(s) 122, 149, 371, 1070, 1471, 1486, 1564, 1582, 1727
NlaIV GGNNCC 5 cut(s) 13, 800, 845, 846, 891
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 4 cut(s) 149, 1070, 1471, 1564
PagI TCATGA 1 cut(s) 118
PaqCI CACCTGC 1 cut(s) 626
PciI ACATGT 2 cut(s) 145, 1066
PciSI GCTCTTC 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 16
PfeI GAWTC 6 cut(s) 115, 380, 610, 938, 1115, 1160
PleI GAGTC 2 cut(s) 311, 1207
PluTI GGCGCC 1 cut(s) 15
PpsI GAGTC 2 cut(s) 311, 1207
PscI ACATGT 2 cut(s) 145, 1066
PshBI ATTAAT 1 cut(s) 666
PsiI TTATAA 1 cut(s) 152
Psp6I CCWGG 3 cut(s) 221, 410, 867
PspGI CCWGG 3 cut(s) 221, 410, 867
PspN4I GGNNCC 5 cut(s) 13, 800, 845, 846, 891
PspOMI GGGCCC 1 cut(s) 843
PspPI GGNCC 3 cut(s) 843, 844, 1031
PstI CTGCAG 3 cut(s) 797, 899, 1252
PvuII CAGCTG 1 cut(s) 1247
RsaI GTAC 8 cut(s) 388, 901, 916, 1337, 1357, 1385, 1497, 1512
RsaNI GTAC 8 cut(s) 387, 900, 915, 1336, 1356, 1384, 1496, 1511
RseI CAYNNNNRTG 2 cut(s) 1472, 1565
SacII CCGCGG 1 cut(s) 286
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 8 cut(s) 32, 161, 666, 726, 804, 831, 980, 1097
Sau3AI GATC 4 cut(s) 487, 1429, 1681, 1735
Sau96I GGNCC 3 cut(s) 843, 844, 1031
SchI GAGTC 2 cut(s) 311, 1208
ScrFI CCNGG 3 cut(s) 223, 412, 869
SduI GDGCHC 4 cut(s) 282, 447, 820, 847
SfaNI GCATC 6 cut(s) 216, 437, 696, 709, 860, 1389
SfcI CTRYAG 3 cut(s) 793, 895, 1248
SfoI GGCGCC 1 cut(s) 13
Sfr303I CCGCGG 1 cut(s) 286
SgrBI CCGCGG 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 1472, 1565
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
SsiI CCGC 6 cut(s) 283, 285, 460, 540, 1193, 1550
SspDI GGCGCC 1 cut(s) 11
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 3 cut(s) 221, 410, 867
StyI CCWWGG 4 cut(s) 105, 135, 501, 948
TaaI ACNGT 4 cut(s) 52, 245, 268, 1331
TaiI ACGT 2 cut(s) 480, 1380
TaqI TCGA 3 cut(s) 494, 1089, 1416
TaqII GACCGA 1 cut(s) 938
TatI WGTACW 6 cut(s) 386, 899, 914, 1355, 1383, 1495
TfiI GAWTC 6 cut(s) 115, 380, 610, 938, 1115, 1160
Tru1I TTAA 8 cut(s) 32, 161, 666, 726, 804, 831, 980, 1097
Tru9I TTAA 8 cut(s) 32, 161, 666, 726, 804, 831, 980, 1097
TscAI CASTG 5 cut(s) 93, 174, 264, 825, 1329
TspDTI ATGAA 6 cut(s) 138, 393, 817, 1041, 1567, 1588
TspRI CASTG 5 cut(s) 93, 174, 264, 825, 1329
Vha464I CTTAAG 1 cut(s) 31
VneI GTGCAC 1 cut(s) 278
VspI ATTAAT 1 cut(s) 666
XapI RAATTY 4 cut(s) 824, 1129, 1654, 1709
XceI RCATGY 4 cut(s) 149, 1070, 1471, 1564
XcmI CCANNNNNNNNNTGG 2 cut(s) 1210, 1471
XspI CTAG 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.