Rw4G010470

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011552
Physical Location & Seq
Reverse (-)
24301467 .. 24307053
5587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010470.1

Sequence Viewer

Length: 1905 bp
ATGGCAGCAGGCGCAGGCAGTCAGCAAAGCTTGAGAAAAGCGTTGAAAACAGTTGGATTGGCGAAAGCCAATGGTGAGTTTAAGGTATTGGATAATGCCATTTCCAAGGCCACAAGACATGATGACCAATTGCCAAAGGAGAAACATGTTATTACAATACTACGTGCAATTCCAGCTTCAAGACCTCGTGCTGAAGTTGCTTATTGCATACATGCTCTAGCCAGGCGCCTTTCTAAGGCTCACAGTTGGAAGGTAGCACTGAAAACCGTGATCGTTATACACCGTGCTCTGAGGGAAGTTGATAATACATTTTGTGACGCTCTAATTAAGTATAGCTGGAGTAGAGGTCATATTCTGAACATATCTCATTCGTGGGATGAAACAAGTCCAAGTGCATGGGGTTATTCTACCTGGGTTCGTTTATATGCATCATATCTCGAAGAACGCCTGGAGTGTTTTCGTGTCCTGAAGTATGATGTTCAGAAAGATCACTTGAGAACCAAGGAACTTGAAACCCCAGACTTACTAAGGCATCTACCTGCCTTGCAACAGCTTCTTTCTCGTCTTCTTGATTGCCAGCCAGAAGTGGGGACTATATGTAATAGCCTGATTCAGTATGCTATTTCAATGGTTGCAGGTGAAAGTGCCAAGTTGTACGCTGCAATTACAGATGGTATTGTCAATTTAGTTGACAAGTTCTTTGAGATGCAGCGCGATGATGCAGTTAAAGCACTTGAAATTTATAAGAAGTCGGGAAGCCAGGCAGAGAGGTTATTTCAGTTCTTTGAGATATGCAGGAGCCTTAATTTTGGACGGGGGCTGAAAACGGTTAGAATTCAACAGCTCCCTGAATCATTCTTGACCACTATGGAAGATTATGTTAAGGACGCCTCCGGTGTTGTAATGCTTGAACTTCCCCCAACCATTGAGGATCAAACTGATGCTCCCAAGGAAACTGTTGTGGTAGAAGGGGACTTGTTAATTGACCATGATGATAATAAGGAACAAAAATCAGCACCTCAACATCCAAGCGACCAGAGTGAGGCTGCTGCGACAACACAAATTTTTGACCTGCTGAGCTTTGATGAAGTTATTCCAGTAACATCAGAGTCAGATGAAAACAATTCCCTGGCACTAGCAATCGTTCCGTCGGAGAATCCGTCAGATTCCAAAACAGATGGTCTCAATTCTACAACCTCCAACTCTAGTTGGGAGATTGAACTTTTTACACCAACTTCTAATGGAGCTGCAGTTCAAGAGACACCATCAACTTCTAATGCAGCTGGAGTTGCAGAAACCAAGTTGGCTGGTGGGCTAGACAGATCGACACTGGACAGTCTGTATGATAATGCAATGGCAAGTACACCAAATCAGAACATGTACTCCCCAGCAGCACTGGCACCCTCTAATCCTTTTGAAGATGTTGCTACTTCTTCATACCAATTCCAAGACCCTTATTATGCTTCCAGCAATGCAACAACCCCAGCAAACTTACAAATGGCTATCATGACCCAACAGCAACAATATTTCATGATGATGCAGCAACAACAAGTGCAGCAACTAGAGTCACCCAGCATTGTCCAAAATCCACCGAGTCCTTACCAAAATCCACCAAGTCCTTTCCAAAATTTACCAAATCCTTTCCAAAACCCACCAAGTCCTTTCCAAAATTTACCAAATCCTTTCCAAAATTCACCAACACCAAGTCCTAGCCAAAATCCACCAACACCAAGTCCTTTCCAAAATCCATCAAGTCCTTTCGAAAATTTACCAAATCCTTTCCAAAATTCACCAACACCAAGTCCTAGCCAAAATCCACCAACACCAAGTCCTTTCCAAAATCCATCAAGTCCTTTCGAAAATTTACCAAATCCTTTCCAAAATTCCACCAACACCAAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

634

Amino Acids

70.11

Weight (kDa)

5.05

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 30 - 296 1.5e-71 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AarI CACCTGC 1 cut(s) 626
Acc36I ACCTGC 3 cut(s) 547, 626, 1080
AccB1I GGYRCC 2 cut(s) 225, 1399
AccII CGCG 1 cut(s) 714
AclWI GGATC 1 cut(s) 939
AcuI CTGAAG 2 cut(s) 213, 488
AcyI GRCGYC 2 cut(s) 226, 888
AfaI GTAC 3 cut(s) 656, 1363, 1382
AfiI CCNNNNNNNGG 3 cut(s) 235, 587, 1042
AflIII ACRYGT 2 cut(s) 145, 1377
AjnI CCWGG 5 cut(s) 221, 410, 447, 759, 1128
AloI GAACNNNNNNTCC 4 cut(s) 1236, 1268, 1367, 1399
AluBI AGCT 8 cut(s) 30, 176, 336, 553, 844, 1080, 1247, 1283
AluI AGCT 8 cut(s) 30, 176, 336, 553, 844, 1080, 1247, 1283
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 2 cut(s) 1187, 1253
AlwI GGATC 1 cut(s) 939
AoxI GGCC 1 cut(s) 108
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
Asp700I GAANNNNTTC 1 cut(s) 1092
AspLEI GCGC 3 cut(s) 14, 228, 714
AsuHPI GGTGA 5 cut(s) 86, 650, 1560, 1686, 1782
AsuII TTCGAA 2 cut(s) 1761, 1857
BanI GGYRCC 2 cut(s) 225, 1399
BauI CACGAG 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 289
BccI CCATC 5 cut(s) 665, 1172, 1273, 1756, 1852
BciT130I CCWGG 5 cut(s) 223, 412, 449, 761, 1130
BcoDI GTCTC 2 cut(s) 1187, 1253
BfaI CTAG 8 cut(s) 218, 1136, 1206, 1316, 1562, 1710, 1806, 1903
BfmI CTRYAG 1 cut(s) 1248
BfoI RGCGCY 1 cut(s) 229
BfuAI ACCTGC 3 cut(s) 547, 626, 1080
BlpI GCTNAGC 1 cut(s) 1076
Bme1390I CCNGG 5 cut(s) 223, 412, 449, 761, 1130
BmiI GGNNCC 3 cut(s) 227, 800, 1401
BmrFI CCNGG 5 cut(s) 223, 412, 449, 761, 1130
BmsI GCATC 6 cut(s) 437, 541, 696, 709, 931, 1527
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 3 cut(s) 358, 470, 1305
Bpu1102I GCTNAGC 1 cut(s) 1076
Bpu14I TTCGAA 2 cut(s) 1761, 1857
BpuEI CTTGAG 2 cut(s) 52, 514
BsaAI YACGTR 1 cut(s) 164
BsaHI GRCGYC 2 cut(s) 226, 888
BsaI GGTCTC 1 cut(s) 1187
BsaJI CCNNGG 5 cut(s) 105, 411, 501, 948, 1128
BsaWI WCCGGW 1 cut(s) 893
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 587, 1042
Bse1I ACTGG 3 cut(s) 1097, 1335, 1401
Bse3DI GCAATG 2 cut(s) 1359, 1477
BseBI CCWGG 5 cut(s) 223, 412, 449, 761, 1130
BseDI CCNNGG 5 cut(s) 105, 411, 501, 948, 1128
BseGI GGATG 2 cut(s) 382, 1024
BseLI CCNNNNNNNGG 3 cut(s) 235, 587, 1042
BseMI GCAATG 2 cut(s) 1359, 1477
BseMII CTCAG 2 cut(s) 281, 1067
BseNI ACTGG 3 cut(s) 1097, 1335, 1401
BseYI CCCAGC 3 cut(s) 1387, 1483, 1571
BsgI GTGCAG 1 cut(s) 1574
Bsh1236I CGCG 1 cut(s) 714
BshFI GGCC 1 cut(s) 110
BshNI GGYRCC 2 cut(s) 225, 1399
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 1 cut(s) 894
BslFI GGGAC 2 cut(s) 604, 986
BslI CCNNNNNNNGG 3 cut(s) 235, 587, 1042
BsmAI GTCTC 2 cut(s) 1187, 1253
BsmFI GGGAC 2 cut(s) 604, 986
BsnI GGCC 1 cut(s) 110
Bso31I GGTCTC 1 cut(s) 1187
Bsp119I TTCGAA 2 cut(s) 1761, 1857
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 4 cut(s) 270, 487, 931, 1322
Bsp1720I GCTNAGC 1 cut(s) 1076
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 2 cut(s) 282, 1068
BspFNI CGCG 1 cut(s) 714
BspHI TCATGA 2 cut(s) 1506, 1530
BspLI GGNNCC 3 cut(s) 227, 800, 1401
BspMAI CTGCAG 1 cut(s) 1252
BspMI ACCTGC 3 cut(s) 547, 626, 1080
BspPI GGATC 1 cut(s) 939
BspT104I TTCGAA 2 cut(s) 1761, 1857
BspT107I GGYRCC 2 cut(s) 225, 1399
BspTNI GGTCTC 1 cut(s) 1187
BsrDI GCAATG 2 cut(s) 1359, 1477
BsrI ACTGG 3 cut(s) 1097, 1335, 1401
BssECI CCNNGG 5 cut(s) 105, 411, 501, 948, 1128
BssMI GATC 4 cut(s) 270, 487, 931, 1322
BssNI GRCGYC 2 cut(s) 226, 888
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 3 cut(s) 105, 501, 948
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 5 cut(s) 223, 412, 449, 761, 1130
Bst4CI ACNGT 7 cut(s) 52, 245, 268, 284, 829, 958, 1337
BstACI GRCGYC 2 cut(s) 226, 888
BstBAI YACGTR 1 cut(s) 164
BstBI TTCGAA 2 cut(s) 1761, 1857
BstC8I GCNNGC 3 cut(s) 10, 16, 578
BstDEI CTNAG 4 cut(s) 234, 290, 527, 1076
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 382, 1024
BstFNI CGCG 1 cut(s) 714
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 3 cut(s) 14, 228, 714
BstKTI GATC 4 cut(s) 273, 490, 934, 1325
BstMAI GTCTC 2 cut(s) 1187, 1253
BstMBI GATC 4 cut(s) 270, 487, 931, 1322
BstMWI GCNNNNNNNGC 6 cut(s) 11, 173, 197, 728, 1289, 1397
BstNI CCWGG 5 cut(s) 223, 412, 449, 761, 1130
BstNSI RCATGY 3 cut(s) 149, 215, 1381
BstSCI CCNGG 5 cut(s) 221, 410, 447, 759, 1128
BstSFI CTRYAG 1 cut(s) 1248
BstUI CGCG 1 cut(s) 714
BstV2I GAAGAC 1 cut(s) 557
BstXI CCANNNNNNTGG 1 cut(s) 396
BsuRI GGCC 1 cut(s) 110
BtgZI GCGATG 1 cut(s) 729
BtsCI GGATG 2 cut(s) 382, 1024
BtsIMutI CAGTG 3 cut(s) 257, 1328, 1394
BveI ACCTGC 3 cut(s) 547, 626, 1080
Cac8I GCNNGC 3 cut(s) 10, 16, 578
CciI TCATGA 2 cut(s) 1506, 1530
CfoI GCGC 3 cut(s) 14, 228, 714
CseI GACGC 2 cut(s) 326, 896
Csp6I GTAC 3 cut(s) 655, 1362, 1381
CviAII CATG 8 cut(s) 119, 146, 212, 396, 989, 1378, 1507, 1531
CviQI GTAC 3 cut(s) 655, 1362, 1381
DdeI CTNAG 4 cut(s) 234, 290, 527, 1076
DinI GGCGCC 1 cut(s) 227
DpnI GATC 4 cut(s) 272, 489, 933, 1324
DpnII GATC 4 cut(s) 270, 487, 931, 1322
Eco130I CCWWGG 3 cut(s) 105, 501, 948
Eco31I GGTCTC 1 cut(s) 1187
Eco57I CTGAAG 2 cut(s) 213, 488
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 834
EcoRII CCWGG 5 cut(s) 221, 410, 447, 759, 1128
EcoT14I CCWWGG 3 cut(s) 105, 501, 948
EcoT22I ATGCAT 1 cut(s) 430
EgeI GGCGCC 1 cut(s) 227
EheI GGCGCC 1 cut(s) 227
ErhI CCWWGG 3 cut(s) 105, 501, 948
FaeI CATG 8 cut(s) 122, 149, 215, 399, 992, 1381, 1510, 1534
FaqI GGGAC 2 cut(s) 604, 986
FatI CATG 8 cut(s) 118, 145, 211, 395, 988, 1377, 1506, 1530
FokI GGATG 2 cut(s) 389, 1011
FspBI CTAG 8 cut(s) 218, 1136, 1206, 1316, 1562, 1710, 1806, 1903
GlaI GCGC 3 cut(s) 13, 227, 713
GsaI CCCAGC 3 cut(s) 1391, 1487, 1575
GsuI CTGGAG 3 cut(s) 358, 470, 1305
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 1 cut(s) 110
HapII CCGG 1 cut(s) 894
HgaI GACGC 2 cut(s) 326, 896
HhaI GCGC 3 cut(s) 14, 228, 714
Hin1I GRCGYC 2 cut(s) 226, 888
Hin1II CATG 8 cut(s) 122, 149, 215, 399, 992, 1381, 1510, 1534
Hin6I GCGC 3 cut(s) 12, 226, 712
HinP1I GCGC 3 cut(s) 12, 226, 712
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 7 cut(s) 610, 851, 1109, 1156, 1166, 1565, 1594
HpaII CCGG 1 cut(s) 894
HphI GGTGA 5 cut(s) 86, 650, 1560, 1686, 1782
Hpy166II GTNNAC 2 cut(s) 691, 1364
Hpy188I TCNGA 8 cut(s) 291, 357, 483, 1108, 1114, 1153, 1165, 1374
Hpy188III TCNNGA 9 cut(s) 180, 437, 466, 569, 753, 859, 1256, 1507, 1531
Hpy8I GTNNAC 2 cut(s) 691, 1364
Hpy99I CGWCG 1 cut(s) 1153
HpyAV CCTTC 2 cut(s) 244, 962
HpyCH4III ACNGT 7 cut(s) 52, 245, 268, 284, 829, 958, 1337
HpyCH4IV ACGT 1 cut(s) 163
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 173, 197, 728, 1289, 1397
HpyF3I CTNAG 4 cut(s) 234, 290, 527, 1076
HpySE526I ACGT 1 cut(s) 163
Hsp92I GRCGYC 2 cut(s) 226, 888
Hsp92II CATG 8 cut(s) 122, 149, 215, 399, 992, 1381, 1510, 1534
HspAI GCGC 3 cut(s) 12, 226, 712
KasI GGCGCC 1 cut(s) 225
Kzo9I GATC 4 cut(s) 270, 487, 931, 1322
LmnI GCTCC 4 cut(s) 798, 849, 949, 1244
LweI GCATC 6 cut(s) 437, 541, 696, 709, 931, 1527
MaeI CTAG 8 cut(s) 218, 1136, 1206, 1316, 1562, 1710, 1806, 1903
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 3 cut(s) 314, 1099, 1566
MalI GATC 4 cut(s) 272, 489, 933, 1324
MboI GATC 4 cut(s) 270, 487, 931, 1322
MboII GAAGA 5 cut(s) 452, 557, 884, 1425, 1430
MfeI CAATTG 1 cut(s) 128
MhlI GDGCHC 1 cut(s) 289
Mly113I GGCGCC 1 cut(s) 226
MlyI GAGTC 3 cut(s) 1118, 1574, 1603
MmeI TCCRAC 4 cut(s) 34, 227, 1131, 1224
Mph1103I ATGCAT 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 1092
MseI TTAA 6 cut(s) 81, 327, 726, 804, 882, 980
MslI CAYNNNNRTG 1 cut(s) 1535
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 1 cut(s) 894
MspR9I CCNGG 5 cut(s) 223, 412, 449, 761, 1130
MunI CAATTG 1 cut(s) 128
MvaI CCWGG 5 cut(s) 223, 412, 449, 761, 1130
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 6 cut(s) 11, 173, 197, 728, 1289, 1397
NarI GGCGCC 1 cut(s) 226
NdeII GATC 4 cut(s) 270, 487, 931, 1322
NlaIII CATG 8 cut(s) 122, 149, 215, 399, 992, 1381, 1510, 1534
NlaIV GGNNCC 3 cut(s) 227, 800, 1401
NmuCI GTSAC 2 cut(s) 314, 1566
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 3 cut(s) 149, 215, 1381
NspV TTCGAA 2 cut(s) 1761, 1857
PagI TCATGA 2 cut(s) 1506, 1530
PaqCI CACCTGC 1 cut(s) 626
PciI ACATGT 2 cut(s) 145, 1377
PcsI WCGNNNNNNNCGW 1 cut(s) 1157
PdmI GAANNNNTTC 1 cut(s) 1092
PfeI GAWTC 4 cut(s) 610, 851, 1156, 1166
PleI GAGTC 3 cut(s) 1117, 1573, 1602
PluTI GGCGCC 1 cut(s) 229
PpsI GAGTC 3 cut(s) 1117, 1573, 1602
Ppu21I YACGTR 1 cut(s) 164
PscI ACATGT 2 cut(s) 145, 1377
PsiI TTATAA 1 cut(s) 744
Psp6I CCWGG 5 cut(s) 221, 410, 447, 759, 1128
PspFI CCCAGC 3 cut(s) 1387, 1483, 1571
PspGI CCWGG 5 cut(s) 221, 410, 447, 759, 1128
PspN4I GGNNCC 3 cut(s) 227, 800, 1401
PstI CTGCAG 1 cut(s) 1252
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 3 cut(s) 656, 1363, 1382
RsaNI GTAC 3 cut(s) 655, 1362, 1381
RseI CAYNNNNRTG 1 cut(s) 1535
SaqAI TTAA 6 cut(s) 81, 327, 726, 804, 882, 980
Sau3AI GATC 4 cut(s) 270, 487, 931, 1322
SchI GAGTC 3 cut(s) 1118, 1574, 1603
ScrFI CCNGG 5 cut(s) 223, 412, 449, 761, 1130
SduI GDGCHC 1 cut(s) 289
SfaNI GCATC 6 cut(s) 437, 541, 696, 709, 931, 1527
SfcI CTRYAG 1 cut(s) 1248
SfoI GGCGCC 1 cut(s) 227
SfuI TTCGAA 2 cut(s) 1761, 1857
SmiMI CAYNNNNRTG 1 cut(s) 1535
SmlI CTYRAG 2 cut(s) 31, 493
SmoI CTYRAG 2 cut(s) 31, 493
SspDI GGCGCC 1 cut(s) 225
SspI AATATT 1 cut(s) 1526
SspMI CTAG 8 cut(s) 218, 1136, 1206, 1316, 1562, 1710, 1806, 1903
StyD4I CCNGG 5 cut(s) 221, 410, 447, 759, 1128
StyI CCWWGG 3 cut(s) 105, 501, 948
TaaI ACNGT 7 cut(s) 52, 245, 268, 284, 829, 958, 1337
TaiI ACGT 1 cut(s) 166
TaqI TCGA 4 cut(s) 438, 1325, 1761, 1857
TatI WGTACW 2 cut(s) 1361, 1380
TfiI GAWTC 4 cut(s) 610, 851, 1156, 1166
Tru1I TTAA 6 cut(s) 81, 327, 726, 804, 882, 980
Tru9I TTAA 6 cut(s) 81, 327, 726, 804, 882, 980
TscAI CASTG 3 cut(s) 264, 1335, 1401
TseFI GTSAC 2 cut(s) 314, 1566
Tsp45I GTSAC 2 cut(s) 314, 1566
TspDTI ATGAA 5 cut(s) 393, 1101, 1131, 1425, 1519
TspGWI ACGGA 2 cut(s) 1137, 1149
TspRI CASTG 3 cut(s) 264, 1335, 1401
XagI CCTNNNNNAGG 1 cut(s) 233
XceI RCATGY 3 cut(s) 149, 215, 1381
XmnI GAANNNNTTC 1 cut(s) 1092
XspI CTAG 8 cut(s) 218, 1136, 1206, 1316, 1562, 1710, 1806, 1903
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.