Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010348

Physical Location & Seq

Genomic Coordinates Reverse (-)

39662383 .. 39674192

Gene Structure 11810 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1280700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3984 bp

ATGAAGCACTTCATGCAGCCGAGAAACGCGATATTGACGGAGATGACGGAGCCTCCCTCGTCGCCAAACCCTATCTCCGCCAAGCCCAAGCCACCTCGGAAGCCCAAGGCCTCCAAGGAGAACGCTCCGCCGTCGGATCCCAACTCCATGATCTCCGATTCTAAACCTTCGCCTGCCGCGAAGTTGAAGGGCCCATTGCCGCCTCGGCCCCCGTCGTCGAACCCTCTCAAGCGGAAGCTCAGCGTTGACTCTCAGACCGAAAACTCCGGCCCTGGAACCTCCGATTCTGGTGTACAGGTAGTGGTGAGAATGCGACCACCACAAAAGGACAAGGACGAAGGAGAGATGATGGTGCAGAAATTATCTAGTGACTCTTTGTCGATAAATGGACAGACCTTCACATTTGACTCGGTCTGCAACACAGATGCATCACAGCTAGATATTTTCCAGCTTGTAGGAGCACCTCTAGTTGAGAATTGTATGGCAGGTTTTAACAGTTCTGTATTTGCATACGGACAGACGGGAAGCGGAAAGACATACACCATGTGGGGTCCAGCCAATGCCTTGCTGGATGAGAATCTATCAAGTGATCAACAAGGGTTAACCCCCCGTGTTTTTGAGTGTCTTTTTGCTCGCTTAAATGAGGAGCAAATCAAGCACGCTGATAAAGAACTCAAGTATCAGTGCCATTGTTCTTTTCTCGAGATTTACAATGAACAAATCACAGATCTTTTGGATCCAAATCAAAAAAGCCTTCAGATAAGAGAAGATGTTAAATCAGGTGTTTATGTCGAAAATCTCACCGAGGAGTGTGTACGTACAATGAAAGATGTGACTCAGCTTCTGATAAAGGGCTTGTCAAACAGGAGAACAGGTTCAACTAGTATCAATGCTGAGAGTTCCCGCTCACATACTGTATTTACCTGTGTTGTTGAATCTCAATGCAAGAATGTGGCAAATGGTACAAGTTTCAAAACCAGTAGAATAAATCTTGTTGATCTAGCTGGATCAGAGCGTCAGAAGTTAACAGGGGCGGCAGGAGAGCGCTTGAAGGAAGCAGGGAATATCAATCGATCACTCTCACAGCTTGGGAACCTGATAAACATTCTTGCTGAAATTTCTCAAACGGGAAAGCAAAGGCATATCCCCTATAGAGATTCCAGGCTTACATTCTTATTGCAGGAATCTCTGGGTGGAAATGCAAAGTTAGCAATGGTCTGTGCTATTTCTCCAACACAAGGCTGTAAGAGTGAGACGTTCAGTACATTGAGGTTTGCACAGCGTGCTAAGGCTATCAAGAACAAGGCAGTTGTTAATGAAGTAATGCAGGAAGATGTGAATCAGTTGCGTGAGGTGATACGACAGCTAAGGGATGAACTACGCCAGATTAAGGCAAATGGAAACAACCCAGTCGCTTCAAGTGGAGGTCACAGTGCAGCATGGTTTCGTCAAAGTTTGAATATGCTAAAGGCTAGCCTTAAACCTCCAATGACATTACCTCATGTTGATGATGACAGTGATGAGGAGATGGAAATTGATGAGGAAGCTGTTGAGAGGCTTTGTGCTGAAGTAAGTAACCAGACAGTTGTCAATGAAGCTAACAACAGAGCAGATGTGAACAGTGTAGACACAATGAAATCACATTCGCAACTCGTGGCTGTTCAAATTGGAAGCTCCGAAGTTTCTCGAAGCTATGCATCTGGAAGTGATTGTATCAAAGAACAAGGTGTTGATACAGATGTTAATATGGAAGAAGGAATATCCGAACAAGGGGACATGATTGTTGAATGTGCTAGCAGCACCCCAGTTATTCCCAGTGCTAGTGCATCGGACCCTCGCAATGTTATAGAAGATACCTCCATGCAGCCTGCTCAGGAGGAAAATATTCTGAGGTCCTCCATTATTGATTTTCTAATTGAAGAATCAGCCTGCAAAAGTGTGGAAGAAGGTTCCTTATGTGCAGCCTCTGATCCTTCAGGAGGCATGCCTGGCTGCCTTTCAGTCACAGATGAATGCATTGGTTCTCCAAATGACGTAATTAACTGTGTTTCTCCATGTCTGAGCATAGTTCCATGCAATGTACCCGAAGTTCTAAAGTCTCCTACCCCCAGTGTTTCACCCAGGGTCAACACCAGCAGGAAAAGTTTGAGGACGTCTTCAATGCTGACTGCTTCTCAGAAGGATCTTACTGGTGGGAGTACGTTAAGTCCAGATGACATGCGCATATCTTTTGGAAAACCTGTAATAAAGAGCTCAGCGAATGACCTATCTGCTCGAGCATTTACTAACTTTTCAGCACCAGCTGAACAGTTGGCAGCAAGCATTCAGCATGGTCTTGAAATTATTGGTAGTCACCGCCAAAGTTCAGCTTTAAGGAAGTCGTCATTCAGGTTTTCATTGAAACCTTCAGAGTCCAGGGTGATTTTCCCCGTTTCCAAAGCTGATGTGGGATTGCAAACTAGTCAAGAAATAGTAAAAGATGATTCAGTGGAGTTTATTTGTAATAATTGCAAGAATAGAATGCAGCTAGAGGTCAAGGAGGCAAATGATATTTCAGATATGCAATTGGTACCTGTTGATGGGTCAGAGTCTGCTGACAAATCCAAGATTCAAGTTCCCAAAGCTGTGGAGAAGGTTTTAGCGGGAGCTATTAGGAGAGAAATGGCATTAGAAGACCTATGTGCCAAGAAAACTTCAGAGATAATGCAGCTAAACCGATTGGTTCAGCAGTACAAGCATGAGAGAGAATGCAATGGCATTATAGCCCAGACAAGGGAGGATAAGATTCTACACCTTGAGAGCCTCATGGATGGTGTTTTACCCACTGAGGAGTTCATGGAGGAAGAGCTAGTGTCTCTCACACATGAGTATAAGATCCTGAAGGAGAAATACGAGAATCACCCAGAACTTTTGAGGACAAATATTGAACTCAAAAGAGTTCAAGATGAGTTGGAAAATTTACGAAACTTCTGTGACATGGGTGAAAGGGAAGTGTTGTTCGAAGAGATTCAAGATCTAAGAAGCCAGCTACAGTATTACGTAGACTGTTCATCCACATCAGCCCGGACACGAAACTCTATGATGCAGTTGACCTATTCACGTGACCCAAATGTGGCCCCTCTCAGTACAATATCAGAGTCAAATGAGGAAAGCTCTGAACAGAAACTAGAACAAGAGAGACAAAAGTGGAATGAAGTAGAGAGTAAGTGGATATCTCTTACTGAGGAATTCAGAATGGAACTTGAGGCCAGCAGATCACTGGCCGAAAAGACAATGCAAGAATTAGAAACTGAGAAGAAATGTGCAGAAGAGCTCAAGAAAGCAATGAAACTGGCTATGGAGGGGCAATCACGGATGTTAGAACAGTATGCAGATTTGGAAGAGAAACATATGCAACTGCTTGCAAGGCATAGAATGATAAGGGATGGAGTGGAGGATGTGAAGAAAGCAGCTGCAAAGGCAGGAGTCAGGGGTGCCGAGTCCAAATTCATAAATGCTATTGCTGCAGAAATTTCAGCATTGAGAGTAGAAAGAGAAAGAGAGGGGCGGTATCTTAGGGACGAAAATAAGGGGATTCAGGCACAATTGCGGGATACTGCTGAAGCTGTGCAGGCTGCAGGCGAATTACTTGTGCGGCTCAAAGAAGCAGATGAGGCTGTTGCTACTGCTCAGAAGCAAGCTATGGAGGCCAAGCAAGAAGCAGACAAAGCTTACATGAAGATTGAAAAACTGAAGAAAAAACATGAGAAAGAGATTAGCAGTCTTAATGAGCTCATTGCCGAGTCTCGTTTACCCAAAGAAGCAATTCAACCTGCTTATGACGGTTCTAATATGGCAAAATATGATGCAGGCGAGCCCCATAGTTTGAGTGATCAAATATGTAGAGAGGAATTTGAACCATTCTACAACGGGGAAGAGGGCGAGTTGCGAAAACTCGCCGAGCCCTCATTATGGTTCTCCGGTTATGACCGATGCAACATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0000280 GO:0000910 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007112 GO:0007140 GO:0007275 GO:0007276 GO:0008150 GO:0008574 GO:0009524 GO:0009555 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033206 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0051301 GO:0051321 GO:0051704 GO:0055046 GO:0061640 GO:0071840 GO:0080175 GO:0140013 GO:1903046 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1328

Amino Acids

147.17

Weight (kDa)

5.22

Isoelectric Point (pI)

48.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	99 - 245	1.1e-20	Microtubule binding
Kinesin	PF00225	120 - 432	1.4e-106	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010348)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23670 AT3G23670 AT4G14150
fragaria_vesca	FvH4_4g12770 FvH4_4g12770
malus_domestica	MD04G1023400.v1.1 MD13G1280700.v1.1
prunus_persica	Prupe.1G173100_v2.0.a1
pyrus_communis	pycom04g01990 pycom13g29270
rosa_chinensis	RchiOBHm_Chr4g0412101
rosa_laevigata	RLG00000008323
rosa_multiflora	Rmu_co8521685.1_g000001 Rmu_sc0004447.1_g000004
rosa_roxburghii	Rroxscaffold_5G00356370
rosa_rugosa	Rorug04G0106600
rosa_samantha	Rh4AG171300 Rh4BG170100 Rh4CG182200 Rh4DG165600
rosa_wichuraiana	Rw4G014280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2156
Acc16I	TGCGCA	1 cut(s)	2222
Acc36I	ACCTGC	2 cut(s)	476, 3820
Acc65I	GGTACC	1 cut(s)	2569
AccB1I	GGYRCC	2 cut(s)	2569, 3473
AccBSI	CCGCTC	1 cut(s)	906
AccI	GTMKAC	2 cut(s)	1626, 3042
AccII	CGCG	2 cut(s)	29, 179
AclWI	GGATC	8 cut(s)	131, 144, 731, 744, 1015, 1964, 2190, 2869
AcoI	YGGCCR	1 cut(s)	3261
AcsI	RAATTY	6 cut(s)	1116, 2956, 3227, 3485, 3510, 3890
AcuI	CTGAAG	8 cut(s)	740, 1587, 1959, 2391, 2679, 2900, 3621, 3752
AcvI	CACGTG	1 cut(s)	3101
AcyI	GRCGYC	1 cut(s)	2153
AdeI	CACNNNGTG	2 cut(s)	546, 1283
AfeI	AGCGCT	1 cut(s)	1046
AfiI	CCNNNNNNNGG	9 cut(s)	1238, 1390, 1770, 1979, 2579, 2772, 2773, 3112, 3951
AhdI	GACNNNNNGTC	1 cut(s)	376
AhlI	ACTAGT	2 cut(s)	881, 2459
AjnI	CCWGG	5 cut(s)	271, 1160, 1987, 2120, 2414
AloI	GAACNNNNNNTCC	4 cut(s)	859, 891, 2981, 3013
Alw21I	GWGCWC	4 cut(s)	463, 2255, 3315, 3774
Alw26I	GTCTC	5 cut(s)	1247, 2103, 2859, 3172, 3789
AlwI	GGATC	8 cut(s)	131, 144, 731, 744, 1015, 1964, 2190, 2869
AlwNI	CAGNNNCTG	3 cut(s)	844, 1967, 2591
Ama87I	CYCGRG	2 cut(s)	701, 2274
Aor51HI	AGCGCT	1 cut(s)	1046
AoxI	GGCC	8 cut(s)	108, 190, 206, 268, 3114, 3246, 3261, 3687
ApaI	GGGCCC	1 cut(s)	194
ApoI	RAATTY	6 cut(s)	1116, 2956, 3227, 3485, 3510, 3890
Asp700I	GAANNNNTTC	5 cut(s)	8, 874, 1884, 3006, 3804
Asp718I	GGTACC	1 cut(s)	2569
AspLEI	GCGC	2 cut(s)	1047, 2223
AspS9I	GGNCC	8 cut(s)	190, 191, 207, 269, 551, 1831, 1893, 3115
AsuC2I	CCSGG	1 cut(s)	3064
AsuHPI	GGTGA	8 cut(s)	316, 793, 1366, 2109, 2345, 2431, 2891, 2993
AsuII	TTCGAA	1 cut(s)	3000
AsuNHI	GCTAGC	2 cut(s)	1472, 1793
AvaI	CYCGRG	2 cut(s)	701, 2274
AvaII	GGWCC	3 cut(s)	551, 1831, 1893
BaeGI	GKGCMC	1 cut(s)	194
BaeI	ACNNNNGTAYC	4 cut(s)	662, 695, 1725, 1758
BamHI	GGATCC	2 cut(s)	136, 736
BanI	GGYRCC	2 cut(s)	2569, 3473
BanII	GRGCYC	6 cut(s)	194, 2255, 3315, 3774, 3858, 3945
BarI	GAAGNNNNNNTAC	2 cut(s)	2873, 2905
BauI	CACGAG	1 cut(s)	1652
BbrPI	CACGTG	1 cut(s)	3101
BbsI	GAAGAC	2 cut(s)	2148, 2679
Bbv12I	GWGCWC	4 cut(s)	463, 2255, 3315, 3774
BccI	CCATC	5 cut(s)	343, 1522, 2573, 2804, 3419
BceAI	ACGGC	1 cut(s)	115
BciT130I	CCWGG	5 cut(s)	273, 1162, 1989, 2122, 2416
BciVI	GTATCC	1 cut(s)	3586
BclI	TGATCA	2 cut(s)	589, 3871
BcnI	CCSGG	1 cut(s)	3064
BcoDI	GTCTC	5 cut(s)	1247, 2103, 2859, 3172, 3789
BcuI	ACTAGT	2 cut(s)	881, 2459
BfmI	CTRYAG	4 cut(s)	1150, 3029, 3504, 3615
BfoI	RGCGCY	1 cut(s)	1048
BfuAI	ACCTGC	2 cut(s)	476, 3820
BfuI	GTATCC	1 cut(s)	3586
BglI	GCCNNNNNGGC	1 cut(s)	205
BglII	AGATCT	2 cut(s)	727, 3013
BlpI	GCTNAGC	2 cut(s)	239, 2254
Bme1390I	CCNGG	6 cut(s)	273, 1162, 1989, 2122, 2416, 3064
Bme18I	GGWCC	3 cut(s)	551, 1831, 1893
BmeRI	GACNNNNNGTC	1 cut(s)	376
BmeT110I	CYCGRG	2 cut(s)	701, 2274
BmgT120I	GGNCC	8 cut(s)	190, 191, 207, 269, 551, 1831, 1893, 3115
BmrFI	CCNGG	6 cut(s)	273, 1162, 1989, 2122, 2416, 3064
BmrI	ACTGGG	4 cut(s)	1403, 1799, 1809, 2103
BmsI	GCATC	7 cut(s)	415, 437, 1706, 1835, 3072, 3835, 3962
BmtI	GCTAGC	2 cut(s)	1476, 1797
BmuI	ACTGGG	4 cut(s)	1403, 1799, 1809, 2103
BoxI	GACNNNNGTC	1 cut(s)	1586
BpiI	GAAGAC	2 cut(s)	2148, 2679
BplI	GAGNNNNNCTC	4 cut(s)	41, 73, 3137, 3169
Bpu10I	CCTNAGC	3 cut(s)	1287, 1367, 1872
Bpu1102I	GCTNAGC	2 cut(s)	239, 2254
Bpu14I	TTCGAA	1 cut(s)	3000
BpuEI	CTTGAG	5 cut(s)	212, 659, 2816, 3263, 3299
BpuMI	CCSGG	1 cut(s)	3064
Bsa29I	ATCGAT	1 cut(s)	1072
BsaAI	YACGTR	3 cut(s)	818, 3040, 3101
BsaBI	GATNNNNATC	3 cut(s)	576, 741, 1338
BsaHI	GRCGYC	1 cut(s)	2153
BsaJI	CCNNGG	9 cut(s)	95, 105, 114, 203, 271, 804, 2120, 2121, 2415
BsaWI	WCCGGW	1 cut(s)	3959
BsaXI	ACNNNNNCTCC	6 cut(s)	37, 59, 67, 89, 859, 889
Bsc4I	CCNNNNNNNGG	9 cut(s)	1238, 1390, 1770, 1979, 2579, 2772, 2773, 3112, 3951
Bse1I	ACTGG	8 cut(s)	978, 1409, 1805, 1815, 2109, 2194, 3264, 3336
Bse3DI	GCAATG	7 cut(s)	194, 1218, 1846, 2083, 2758, 3330, 3774
Bse8I	GATNNNNATC	3 cut(s)	576, 741, 1338
BseBI	CCWGG	5 cut(s)	273, 1162, 1989, 2122, 2416
BseCI	ATCGAT	1 cut(s)	1072
BseDI	CCNNGG	9 cut(s)	95, 105, 114, 203, 271, 804, 2120, 2121, 2415
BseGI	GGATG	7 cut(s)	577, 1378, 2815, 3050, 3360, 3430, 3442
BseJI	GATNNNNATC	3 cut(s)	576, 741, 1338
BseLI	CCNNNNNNNGG	9 cut(s)	1238, 1390, 1770, 1979, 2579, 2772, 2773, 3112, 3951
BseMI	GCAATG	7 cut(s)	194, 1218, 1846, 2083, 2758, 3330, 3774
BseNI	ACTGG	8 cut(s)	978, 1409, 1805, 1815, 2109, 2194, 3264, 3336
BseRI	GAGGAG	4 cut(s)	659, 821, 1538, 2843
BseSI	GKGCMC	1 cut(s)	194
BsgI	GTGCAG	5 cut(s)	374, 1455, 1980, 3324, 3629
Bsh1236I	CGCG	2 cut(s)	29, 179
BshFI	GGCC	8 cut(s)	110, 192, 208, 270, 3116, 3248, 3263, 3689
BshNI	GGYRCC	2 cut(s)	2569, 3473
BshVI	ATCGAT	1 cut(s)	1072
BsiHKAI	GWGCWC	4 cut(s)	463, 2255, 3315, 3774
BsiHKCI	CYCGRG	2 cut(s)	701, 2274
BsiSI	CCGG	3 cut(s)	267, 3064, 3960
BslFI	GGGAC	2 cut(s)	1787, 3572
BslI	CCNNNNNNNGG	9 cut(s)	1238, 1390, 1770, 1979, 2579, 2772, 2773, 3112, 3951
BsmAI	GTCTC	5 cut(s)	1247, 2103, 2859, 3172, 3789
BsmBI	CGTCTC	1 cut(s)	1247
BsmFI	GGGAC	2 cut(s)	1787, 3572
BsmI	GAATGC	5 cut(s)	315, 2018, 2322, 2526, 2753
BsnI	GGCC	8 cut(s)	110, 192, 208, 270, 3116, 3248, 3263, 3689
BsoBI	CYCGRG	2 cut(s)	701, 2274
Bsp119I	TTCGAA	1 cut(s)	3000
Bsp120I	GGGCCC	1 cut(s)	190
Bsp1286I	GDGCHC	7 cut(s)	194, 463, 2255, 3315, 3774, 3858, 3945
Bsp1407I	TGTACA	1 cut(s)	292
Bsp1720I	GCTNAGC	2 cut(s)	239, 2254
BspANI	GGCC	8 cut(s)	110, 192, 208, 270, 3116, 3248, 3263, 3689
BspDI	ATCGAT	1 cut(s)	1072
BspFNI	CGCG	2 cut(s)	29, 179
BspMAI	CTGCAG	2 cut(s)	3508, 3619
BspMI	ACCTGC	2 cut(s)	476, 3820
BspOI	GCTAGC	2 cut(s)	1476, 1797
BspPI	GGATC	8 cut(s)	131, 144, 731, 744, 1015, 1964, 2190, 2869
BspQI	GCTCTTC	2 cut(s)	2838, 3303
BspT104I	TTCGAA	1 cut(s)	3000
BspT107I	GGYRCC	2 cut(s)	2569, 3473
BsrBI	CCGCTC	1 cut(s)	906
BsrDI	GCAATG	7 cut(s)	194, 1218, 1846, 2083, 2758, 3330, 3774
BsrGI	TGTACA	1 cut(s)	292
BsrI	ACTGG	8 cut(s)	978, 1409, 1805, 1815, 2109, 2194, 3264, 3336
BssECI	CCNNGG	9 cut(s)	95, 105, 114, 203, 271, 804, 2120, 2121, 2415
BssNI	GRCGYC	1 cut(s)	2153
BssSI	CACGAG	1 cut(s)	1652
BssT1I	CCWWGG	2 cut(s)	105, 114
Bst2BI	CACGAG	1 cut(s)	1652
Bst2UI	CCWGG	5 cut(s)	273, 1162, 1989, 2122, 2416
Bst6I	CTCTTC	5 cut(s)	2838, 2997, 3303, 3375, 3909
BstACI	GRCGYC	1 cut(s)	2153
BstAPI	GCANNNNNTGC	2 cut(s)	13, 1283
BstAUI	TGTACA	1 cut(s)	292
BstBAI	YACGTR	3 cut(s)	818, 3040, 3101
BstBI	TTCGAA	1 cut(s)	3000
BstENI	CCTNNNNNAGG	1 cut(s)	1977
BstF5I	GGATG	7 cut(s)	577, 1378, 2815, 3050, 3360, 3430, 3442
BstFNI	CGCG	2 cut(s)	29, 179
BstH2I	RGCGCY	1 cut(s)	1048
BstHHI	GCGC	2 cut(s)	1047, 2223
BstMAI	GTCTC	5 cut(s)	1247, 2103, 2859, 3172, 3789
BstNI	CCWGG	5 cut(s)	273, 1162, 1989, 2122, 2416
BstNSI	RCATGY	2 cut(s)	1987, 2221
BstPAI	GACNNNNGTC	1 cut(s)	1586
BstSCI	CCNGG	6 cut(s)	271, 1160, 1987, 2120, 2414, 3062
BstSFI	CTRYAG	4 cut(s)	1150, 3029, 3504, 3615
BstSLI	GKGCMC	1 cut(s)	194
BstSNI	TACGTA	2 cut(s)	818, 3040
BstUI	CGCG	2 cut(s)	29, 179
BstV2I	GAAGAC	2 cut(s)	2148, 2679
BstX2I	RGATCY	6 cut(s)	136, 727, 736, 2182, 2874, 3013
BstXI	CCANNNNNNTGG	1 cut(s)	2626
BstYI	RGATCY	6 cut(s)	136, 727, 736, 2182, 2874, 3013
Bsu15I	ATCGAT	1 cut(s)	1072
BsuI	GTATCC	1 cut(s)	3586
BsuRI	GGCC	8 cut(s)	110, 192, 208, 270, 3116, 3248, 3263, 3689
BsuTUI	ATCGAT	1 cut(s)	1072
BtsCI	GGATG	7 cut(s)	577, 1378, 2815, 3050, 3360, 3430, 3442
BtsIMutI	CAGTG	9 cut(s)	689, 1438, 1522, 1627, 1822, 2116, 2493, 2823, 3257
BveI	ACCTGC	2 cut(s)	476, 3820
CaiI	CAGNNNCTG	3 cut(s)	844, 1967, 2591
CfoI	GCGC	2 cut(s)	1047, 2223
Cfr13I	GGNCC	8 cut(s)	190, 191, 207, 269, 551, 1831, 1893, 3115
ClaI	ATCGAT	1 cut(s)	1072
CseI	GACGC	1 cut(s)	1004
DraIII	CACNNNGTG	2 cut(s)	546, 1283
DriI	GACNNNNNGTC	1 cut(s)	376
EaeI	YGGCCR	1 cut(s)	3261
Eam1104I	CTCTTC	5 cut(s)	2838, 2997, 3303, 3375, 3909
Eam1105I	GACNNNNNGTC	1 cut(s)	376
EarI	CTCTTC	5 cut(s)	2838, 2997, 3303, 3375, 3909
EciI	GGCGGA	2 cut(s)	67, 117
Ecl136II	GAGCTC	3 cut(s)	2253, 3313, 3772
Eco105I	TACGTA	2 cut(s)	818, 3040
Eco130I	CCWWGG	2 cut(s)	105, 114
Eco147I	AGGCCT	1 cut(s)	110
Eco24I	GRGCYC	6 cut(s)	194, 2255, 3315, 3774, 3858, 3945
Eco32I	GATATC	1 cut(s)	3213
Eco47I	GGWCC	3 cut(s)	551, 1831, 1893
Eco47III	AGCGCT	1 cut(s)	1046
Eco53kI	GAGCTC	3 cut(s)	2253, 3313, 3772
Eco57I	CTGAAG	8 cut(s)	740, 1587, 1959, 2391, 2679, 2900, 3621, 3752
Eco72I	CACGTG	1 cut(s)	3101
Eco88I	CYCGRG	2 cut(s)	701, 2274
EcoICRI	GAGCTC	3 cut(s)	2253, 3313, 3772
EcoNI	CCTNNNNNAGG	1 cut(s)	1977
EcoO109I	RGGNCCY	2 cut(s)	190, 1893
EcoRI	GAATTC	1 cut(s)	3227
EcoRII	CCWGG	5 cut(s)	271, 1160, 1987, 2120, 2414
EcoRV	GATATC	1 cut(s)	3213
EcoT14I	CCWWGG	2 cut(s)	105, 114
EcoT22I	ATGCAT	3 cut(s)	430, 1699, 2018
EcoT38I	GRGCYC	6 cut(s)	194, 2255, 3315, 3774, 3858, 3945
ErhI	CCWWGG	2 cut(s)	105, 114
Esp3I	CGTCTC	1 cut(s)	1247
FalI	AAGNNNNNCTT	2 cut(s)	2353, 2385
FaqI	GGGAC	2 cut(s)	1787, 3572
FauI	CCCGC	3 cut(s)	911, 2635, 3582
FauNDI	CATATG	1 cut(s)	3390
FbaI	TGATCA	2 cut(s)	589, 3871
FblI	GTMKAC	2 cut(s)	1626, 3042
FokI	GGATG	7 cut(s)	584, 1385, 2822, 3037, 3367, 3437, 3449
FriOI	GRGCYC	6 cut(s)	194, 2255, 3315, 3774, 3858, 3945
FspAI	RTGCGCAY	1 cut(s)	2222
FspI	TGCGCA	1 cut(s)	2222
GlaI	GCGC	2 cut(s)	1046, 2222
HaeII	RGCGCY	1 cut(s)	1048
HaeIII	GGCC	8 cut(s)	110, 192, 208, 270, 3116, 3248, 3263, 3689
HapII	CCGG	3 cut(s)	267, 3064, 3960
HgaI	GACGC	1 cut(s)	1004
HhaI	GCGC	2 cut(s)	1047, 2223
Hin1I	GRCGYC	1 cut(s)	2153
Hin6I	GCGC	2 cut(s)	1045, 2221
HinP1I	GCGC	2 cut(s)	1045, 2221
HincII	GTYRAC	5 cut(s)	247, 603, 1026, 2128, 3090
HindII	GTYRAC	5 cut(s)	247, 603, 1026, 2128, 3090
HindIII	AAGCTT	1 cut(s)	3708
HpaI	GTTAAC	2 cut(s)	603, 1026
HpaII	CCGG	3 cut(s)	267, 3064, 3960
HphI	GGTGA	8 cut(s)	316, 793, 1366, 2109, 2345, 2431, 2891, 2993
Hpy99I	CGWCG	4 cut(s)	64, 136, 217, 220
HpyCH4IV	ACGT	7 cut(s)	817, 1256, 2034, 2153, 2201, 3039, 3100
HpySE526I	ACGT	7 cut(s)	817, 1256, 2034, 2153, 2201, 3039, 3100
Hsp92I	GRCGYC	1 cut(s)	2153
HspAI	GCGC	2 cut(s)	1045, 2221
KpnI	GGTACC	1 cut(s)	2573
Ksp22I	TGATCA	2 cut(s)	589, 3871
KspAI	GTTAAC	2 cut(s)	603, 1026
LguI	GCTCTTC	2 cut(s)	2838, 3303
LmnI	GCTCC	6 cut(s)	49, 130, 458, 646, 1679, 2645
LweI	GCATC	7 cut(s)	415, 437, 1706, 1835, 3072, 3835, 3962
MaeII	ACGT	7 cut(s)	817, 1256, 2034, 2153, 2201, 3039, 3100
MaeIII	GTNAC	8 cut(s)	368, 832, 1427, 1574, 2002, 2351, 2972, 3101
MbiI	CCGCTC	1 cut(s)	906
MfeI	CAATTG	2 cut(s)	2564, 3584
MflI	RGATCY	6 cut(s)	136, 727, 736, 2182, 2874, 3013
MhlI	GDGCHC	7 cut(s)	194, 463, 2255, 3315, 3774, 3858, 3945
MmeI	TCCRAC	3 cut(s)	114, 1256, 2931
Mph1103I	ATGCAT	3 cut(s)	430, 1699, 2018
MroXI	GAANNNNTTC	5 cut(s)	8, 874, 1884, 3006, 3804
MslI	CAYNNNNRTG	1 cut(s)	1506
MspA1I	CMGCKG	2 cut(s)	2303, 3452
MspI	CCGG	3 cut(s)	267, 3064, 3960
MspR9I	CCNGG	6 cut(s)	273, 1162, 1989, 2122, 2416, 3064
MunI	CAATTG	2 cut(s)	2564, 3584
Mva1269I	GAATGC	5 cut(s)	315, 2018, 2322, 2526, 2753
MvaI	CCWGG	5 cut(s)	273, 1162, 1989, 2122, 2416
MvnI	CGCG	2 cut(s)	29, 179
NciI	CCSGG	1 cut(s)	3064
NdeI	CATATG	1 cut(s)	3390
NheI	GCTAGC	2 cut(s)	1472, 1793
NmeAIII	GCCGAG	5 cut(s)	45, 184, 3502, 3805, 3964
NmuCI	GTSAC	7 cut(s)	368, 832, 1427, 2002, 2351, 2972, 3101
NsbI	TGCGCA	1 cut(s)	2222
NsiI	ATGCAT	3 cut(s)	430, 1699, 2018
NspI	RCATGY	2 cut(s)	1987, 2221
NspV	TTCGAA	1 cut(s)	3000
PaeI	GCATGC	1 cut(s)	1987
PaeR7I	CTCGAG	2 cut(s)	701, 2274
PasI	CCCWGGG	1 cut(s)	2121
PceI	AGGCCT	1 cut(s)	110
PciSI	GCTCTTC	2 cut(s)	2838, 3303
PcsI	WCGNNNNNNNCGW	1 cut(s)	176
PctI	GAATGC	5 cut(s)	315, 2018, 2322, 2526, 2753
PdmI	GAANNNNTTC	5 cut(s)	8, 874, 1884, 3006, 3804
PflFI	GACNNNGTC	1 cut(s)	410
PmaCI	CACGTG	1 cut(s)	3101
PmlI	CACGTG	1 cut(s)	3101
Ppu21I	YACGTR	3 cut(s)	818, 3040, 3101
PpuMI	RGGWCCY	1 cut(s)	1893
PshAI	GACNNNNGTC	1 cut(s)	1586
Psp124BI	GAGCTC	3 cut(s)	2255, 3315, 3774
Psp5II	RGGWCCY	1 cut(s)	1893
Psp6I	CCWGG	5 cut(s)	271, 1160, 1987, 2120, 2414
PspCI	CACGTG	1 cut(s)	3101
PspGI	CCWGG	5 cut(s)	271, 1160, 1987, 2120, 2414
PspOMI	GGGCCC	1 cut(s)	190
PspPI	GGNCC	8 cut(s)	190, 191, 207, 269, 551, 1831, 1893, 3115
PspPPI	RGGWCCY	1 cut(s)	1893
PspXI	VCTCGAGB	1 cut(s)	2274
PsrI	GAACNNNNNNTAC	2 cut(s)	2073, 2105
PstI	CTGCAG	2 cut(s)	3508, 3619
PstNI	CAGNNNCTG	3 cut(s)	844, 1967, 2591
PsuI	RGATCY	6 cut(s)	136, 727, 736, 2182, 2874, 3013
PsyI	GACNNNGTC	1 cut(s)	410
PvuII	CAGCTG	2 cut(s)	2303, 3452
RseI	CAYNNNNRTG	1 cut(s)	1506
SacI	GAGCTC	3 cut(s)	2255, 3315, 3774
SapI	GCTCTTC	2 cut(s)	2838, 3303
Sau96I	GGNCC	8 cut(s)	190, 191, 207, 269, 551, 1831, 1893, 3115
ScrFI	CCNGG	6 cut(s)	273, 1162, 1989, 2122, 2416, 3064
SduI	GDGCHC	7 cut(s)	194, 463, 2255, 3315, 3774, 3858, 3945
SfaNI	GCATC	7 cut(s)	415, 437, 1706, 1835, 3072, 3835, 3962
SfcI	CTRYAG	4 cut(s)	1150, 3029, 3504, 3615
Sfr274I	CTCGAG	2 cut(s)	701, 2274
SfuI	TTCGAA	1 cut(s)	3000
SinI	GGWCC	3 cut(s)	551, 1831, 1893
SlaI	CTCGAG	2 cut(s)	701, 2274
SmiMI	CAYNNNNRTG	1 cut(s)	1506
SmlI	CTYRAG	7 cut(s)	227, 674, 701, 2274, 2795, 3242, 3314
SmoI	CTYRAG	7 cut(s)	227, 674, 701, 2274, 2795, 3242, 3314
SnaBI	TACGTA	2 cut(s)	818, 3040
SpeI	ACTAGT	2 cut(s)	881, 2459
SphI	GCATGC	1 cut(s)	1987
SseBI	AGGCCT	1 cut(s)	110
SspI	AATATT	2 cut(s)	1885, 2923
SstI	GAGCTC	3 cut(s)	2255, 3315, 3774
StuI	AGGCCT	1 cut(s)	110
StyD4I	CCNGG	6 cut(s)	271, 1160, 1987, 2120, 2414, 3062
StyI	CCWWGG	2 cut(s)	105, 114
TaiI	ACGT	7 cut(s)	820, 1259, 2037, 2156, 2204, 3042, 3103
TaqI	TCGA	8 cut(s)	218, 380, 702, 792, 1072, 1687, 2275, 3000
TaqII	GACCGA	3 cut(s)	272, 400, 3984
TatI	WGTACW	4 cut(s)	292, 1262, 2730, 3125
TauI	GCSGC	4 cut(s)	179, 202, 1037, 3637
TscAI	CASTG	9 cut(s)	689, 1438, 1522, 1627, 1822, 2116, 2493, 2830, 3264
TseFI	GTSAC	7 cut(s)	368, 832, 1427, 2002, 2351, 2972, 3101
Tsp45I	GTSAC	7 cut(s)	368, 832, 1427, 2002, 2351, 2972, 3101
TspGWI	ACGGA	4 cut(s)	53, 62, 528, 3367
TspRI	CASTG	9 cut(s)	689, 1438, 1522, 1627, 1822, 2116, 2493, 2830, 3264
Tth111I	GACNNNGTC	1 cut(s)	410
VpaK11BI	GGWCC	3 cut(s)	551, 1831, 1893
XagI	CCTNNNNNAGG	1 cut(s)	1977
XapI	RAATTY	6 cut(s)	1116, 2956, 3227, 3485, 3510, 3890
XceI	RCATGY	2 cut(s)	1987, 2221
XcmI	CCANNNNNNNNNTGG	3 cut(s)	565, 2443, 3256
XhoI	CTCGAG	2 cut(s)	701, 2274
XmiI	GTMKAC	2 cut(s)	1626, 3042
XmnI	GAANNNNTTC	5 cut(s)	8, 874, 1884, 3006, 3804
ZraI	GACGTC	1 cut(s)	2154
Zsp2I	ATGCAT	3 cut(s)	430, 1699, 2018

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.