MD14G1003800.v1.1

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006099
Physical Location & Seq
Forward (+)
448951 .. 458196
9246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1003800.v1.1.491

Sequence Viewer

Length: 1812 bp
ATGGAAGTGTTCAGGACGGCCATTTTGCAGCCTGGCCCACCTGAAAGGTTTGCCCTTCAGACAGTTCAACAAGTTATACAACCTCAGAAACAAACAAAGTTAGTCCAAGATGAGAATCAATTGTTAGAAAATATCCTTCGGACATTGCTTCAGGAACTGGTGTCTTCTGCAGTACAGTCTGGGGAGCAGATAATGCAGTATGGTCAATCAATTGATGCTGGAGAAACTGCCCAAGGACATATTCCACGTCTTCTTGACGTTGTGTTGTATCTTTGTGAGAAAGAGCATGTCGAAGGTGGCATGATCTTTCAGCTGTTGGAAGACTTAACAGAAATGTCAACTATGAGAAATTGCAAAGACGTTTTCGGTTATATTGAGAGTAAACAAGATATATTAGGAAAGCCTGAGCTTTTTGCGCGAGGAAAACTTGTGATGTTGAGAACATGCAATCAACTTCTTCGTCGTCTGTCAAAGGCAAATGATGTGGTATTTTGTGGACGAATTCTAATGTTTTTGGCACACTTTTTCCCATTATCTGAGCGTTCAGCTGTTAATATAAAAGGAGTTTTTAACACATCAAATGAGACAAAATATGAGAAAGACCCCCCTGATGGAATTTCCATCGATTTCAACTTTTACAAAACCTTTTGGAGTTTACAGGAAAGCTTCTGTAACCCTCCTTCCTTAACTCTTGGTCCTACCAAGTGGAAGAAATTTACATCCGGTTTAATGGTTGTTTTGAATACCTTTGAAGCTCAACCATTAAGTGATGAAGAGGGAGATGCCAATAACTTGGAGGAGGAAGCAACAAATTTTAGCATAAAATATCTTACAAGCAGTAAACTGATGGGTTTGGAGTTGAAGGATCCCAGTTTCCGACGTCACATTCTTGTGCAGTGCCTCATATTGTTCGATTATCTGAAGGCCCCGGGAAAAAGTGAAAAAGATTTACCTTCTGAAAGCATGAAAGAGGAAATAAAATCTTGTGAGGAGCGTGTCAAAAAGCTGCTTGAGATGACTCCGCCCAAAGGGAAGGAATTTCTTAATAAAATTGAGCATATATTGGAACGTGAAAGGAATTGGGTGTGGTGGAAATGTGACGGTTGTCCCCCATTTGAGAAGCAGCCAATGGAGAAAAAGGTGATCCAAGAAGGAGCCAAAAAGCGTAGGCCGAGATGGAGAATGGGAAATAAAGAACTATCTCAGTTGTGGAAGTGGGCAGATCAGAATCCGAATGCTTTAACCGATCCTCAACGTGTTCGAACACCTTCCATCACTGGGTACTGGAAGCCCTTGGTTGAAGATATGGATCCAGCAGCTGGAATAGAACCTGAGTATCACCACAAAAATAACCGAGTTTATTGCTGGAAAGGTCTTCGATTTTCAGCTAGACAAGACTTGGAAGGATTTTCTCGATTTACTGAACATGGTATTGAAGGAGTTGTACCGCTGGAACTTTTGTCTCCTGAGGAGCGGTCTAAATACCAAGCTAAACCAAATGACAGGTCTAAACGTGCTAAAAAGGAAGAATCAAAAGGTGCTGCACATCAATTAGAAGAAAGTCAGAATGCTACACCTGCAAGTGAATTAGACGGTGAAGCCTCAGGGACGCCAATGGACACTGATACCATTGCTGCAGCCGGCAACATGTCCCAAGGTGGAAGTCCAATCCCTGATGAACATCAGAAGCAAAGCTCCGATGCGGATGTGGGTCAAGAGGGAGGTCAGTTGGAAGCAGATGCCGAGGTCGAGGTTGAGGCAGGGATGCTTGATGGTGAGGACGCGGAGGCTGATTTGGATCCAGTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0023052 GO:0030422 GO:0031047 GO:0031050 GO:0031503 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035556 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071369 GO:0071407 GO:0071426 GO:0071427 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0098542 GO:1901360 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
ko03013 ko03040 map03013 map03040
Pfam Domains
Protein Families
efThoc1

Protein Analysis

604

Amino Acids

68.3

Weight (kDa)

5.17

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
efThoc1 PF11957 83 - 508 1.5e-99 THO complex subunit 1 transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1585
AatII GACGTC 1 cut(s) 883
Acc36I ACCTGC 1 cut(s) 1585
AccB7I CCANNNNNTGG 1 cut(s) 1319
AccBSI CCGCTC 1 cut(s) 1474
AccII CGCG 2 cut(s) 418, 1784
AciI CCGC 5 cut(s) 1022, 1448, 1474, 1703, 1784
AclWI GGATC 8 cut(s) 860, 873, 1138, 1241, 1304, 1317, 1793, 1806
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 5 cut(s) 501, 615, 713, 811, 1037
AcuI CTGAAG 3 cut(s) 41, 134, 941
AcyI GRCGYC 2 cut(s) 880, 1610
AfaI GTAC 3 cut(s) 174, 1283, 1446
AfiI CCNNNNNNNGG 4 cut(s) 611, 1028, 1278, 1319
AflIII ACRYGT 2 cut(s) 1255, 1647
AgsI TTSAA 7 cut(s) 68, 631, 742, 752, 862, 1301, 1436
AjiI CACGTC 1 cut(s) 248
AjnI CCWGG 1 cut(s) 31
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
Alw26I GTCTC 2 cut(s) 578, 1467
AlwI GGATC 8 cut(s) 860, 873, 1138, 1241, 1304, 1317, 1793, 1806
AlwNI CAGNNNCTG 2 cut(s) 157, 1319
Ama87I CYCGRG 1 cut(s) 928
AoxI GGCC 4 cut(s) 18, 34, 924, 1169
ApeKI GCWGC 7 cut(s) 28, 1006, 1123, 1316, 1541, 1634, 1637
ApoI RAATTY 5 cut(s) 501, 615, 713, 811, 1037
ArsI GACNNNNNNTTYG 4 cut(s) 7, 39, 1490, 1522
Asp700I GAANNNNTTC 1 cut(s) 1267
AspLEI GCGC 1 cut(s) 418
AspS9I GGNCC 3 cut(s) 35, 695, 925
AsuC2I CCSGG 2 cut(s) 929, 930
AsuHPI GGTGA 4 cut(s) 1153, 1331, 1607, 1787
AsuII TTCGAA 1 cut(s) 1261
AvaI CYCGRG 1 cut(s) 928
AvaII GGWCC 1 cut(s) 695
AxyI CCTNAGG 2 cut(s) 1467, 1603
BaeI ACNNNNGTAYC 2 cut(s) 1319, 1352
BamHI GGATCC 3 cut(s) 865, 1309, 1798
BarI GAAGNNNNNNTAC 4 cut(s) 664, 696, 1428, 1460
BbsI GAAGAC 4 cut(s) 156, 242, 327, 1367
BbvI GCAGC 7 cut(s) 40, 993, 1135, 1328, 1528, 1621, 1649
BccI CCATC 6 cut(s) 605, 629, 841, 1170, 1280, 1766
BceAI ACGGC 1 cut(s) 33
BcgI CGANNNNNNTGC 2 cut(s) 1344, 1378
BciT130I CCWGG 1 cut(s) 33
BcnI CCSGG 2 cut(s) 929, 930
BcoDI GTCTC 2 cut(s) 578, 1467
BfaI CTAG 1 cut(s) 1389
BfmI CTRYAG 2 cut(s) 168, 1635
BfuAI ACCTGC 1 cut(s) 1585
BisI GCNGC 7 cut(s) 29, 1007, 1124, 1317, 1542, 1635, 1638
BlsI GCNGC 7 cut(s) 30, 1008, 1125, 1318, 1543, 1636, 1639
Bme1390I CCNGG 3 cut(s) 33, 929, 930
Bme18I GGWCC 1 cut(s) 695
BmeT110I CYCGRG 1 cut(s) 928
BmgBI CACGTC 1 cut(s) 248
BmgT120I GGNCC 3 cut(s) 35, 695, 925
BmiI GGNNCC 5 cut(s) 867, 927, 1156, 1311, 1800
BmrFI CCNGG 3 cut(s) 33, 929, 930
BmrI ACTGGG 2 cut(s) 864, 1287
BmsI GCATC 5 cut(s) 205, 772, 1690, 1729, 1755
BmuI ACTGGG 2 cut(s) 864, 1287
BoxI GACNNNNGTC 1 cut(s) 1104
BpiI GAAGAC 4 cut(s) 156, 242, 327, 1367
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 405
Bpu14I TTCGAA 1 cut(s) 1261
BpuEI CTTGAG 1 cut(s) 1031
BpuMI CCSGG 2 cut(s) 929, 930
Bsa29I ATCGAT 1 cut(s) 624
BsaBI GATNNNNATC 5 cut(s) 114, 1227, 1308, 1680, 1797
BsaHI GRCGYC 2 cut(s) 880, 1610
BsaJI CCNNGG 6 cut(s) 232, 927, 928, 1293, 1654, 1743
BsaWI WCCGGW 1 cut(s) 722
Bsc4I CCNNNNNNNGG 4 cut(s) 611, 1028, 1278, 1319
Bse118I RCCGGY 1 cut(s) 1640
Bse1I ACTGG 5 cut(s) 162, 870, 1282, 1289, 1802
Bse21I CCTNAGG 2 cut(s) 1467, 1603
Bse3DI GCAATG 2 cut(s) 143, 1629
Bse8I GATNNNNATC 5 cut(s) 114, 1227, 1308, 1680, 1797
BseBI CCWGG 1 cut(s) 33
BseCI ATCGAT 1 cut(s) 624
BseDI CCNNGG 6 cut(s) 232, 927, 928, 1293, 1654, 1743
BseGI GGATG 3 cut(s) 719, 1711, 1770
BseJI GATNNNNATC 5 cut(s) 114, 1227, 1308, 1680, 1797
BseLI CCNNNNNNNGG 4 cut(s) 611, 1028, 1278, 1319
BseMI GCAATG 2 cut(s) 143, 1629
BseMII CTCAG 7 cut(s) 98, 396, 528, 1217, 1323, 1458, 1617
BseNI ACTGG 5 cut(s) 162, 870, 1282, 1289, 1802
BseRI GAGGAG 3 cut(s) 812, 1004, 1484
BseXI GCAGC 7 cut(s) 40, 993, 1135, 1328, 1528, 1621, 1649
BsgI GTGCAG 2 cut(s) 914, 1527
Bsh1236I CGCG 2 cut(s) 418, 1784
BshFI GGCC 4 cut(s) 20, 36, 926, 1171
BshVI ATCGAT 1 cut(s) 624
BsiHKCI CYCGRG 1 cut(s) 928
BsiSI CCGG 3 cut(s) 723, 929, 1641
BslFI GGGAC 3 cut(s) 1092, 1621, 1636
BslI CCNNNNNNNGG 4 cut(s) 611, 1028, 1278, 1319
BsmAI GTCTC 2 cut(s) 578, 1467
BsmFI GGGAC 3 cut(s) 1092, 1621, 1636
BsmI GAATGC 2 cut(s) 1240, 1573
BsnI GGCC 4 cut(s) 20, 36, 926, 1171
BsoBI CYCGRG 1 cut(s) 928
Bsp119I TTCGAA 1 cut(s) 1261
Bsp143I GATC 7 cut(s) 303, 865, 1143, 1222, 1246, 1309, 1798
BspACI CCGC 5 cut(s) 1022, 1448, 1474, 1703, 1784
BspANI GGCC 4 cut(s) 20, 36, 926, 1171
BspCNI CTCAG 7 cut(s) 97, 397, 529, 1216, 1324, 1459, 1616
BspDI ATCGAT 1 cut(s) 624
BspFNI CGCG 2 cut(s) 418, 1784
BspLI GGNNCC 5 cut(s) 867, 927, 1156, 1311, 1800
BspMAI CTGCAG 2 cut(s) 172, 1639
BspMI ACCTGC 1 cut(s) 1585
BspPI GGATC 8 cut(s) 860, 873, 1138, 1241, 1304, 1317, 1793, 1806
BspT104I TTCGAA 1 cut(s) 1261
BsrBI CCGCTC 1 cut(s) 1474
BsrDI GCAATG 2 cut(s) 143, 1629
BsrFI RCCGGY 1 cut(s) 1640
BsrI ACTGG 5 cut(s) 162, 870, 1282, 1289, 1802
BssAI RCCGGY 1 cut(s) 1640
BssECI CCNNGG 6 cut(s) 232, 927, 928, 1293, 1654, 1743
BssMI GATC 7 cut(s) 303, 865, 1143, 1222, 1246, 1309, 1798
BssNI GRCGYC 2 cut(s) 880, 1610
BssT1I CCWWGG 3 cut(s) 232, 1293, 1654
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 4 cut(s) 64, 177, 1103, 1595
Bst6I CTCTTC 1 cut(s) 768
BstACI GRCGYC 2 cut(s) 880, 1610
BstAPI GCANNNNNTGC 1 cut(s) 193
BstBI TTCGAA 1 cut(s) 1261
BstC8I GCNNGC 1 cut(s) 1642
BstDEI CTNAG 7 cut(s) 84, 405, 537, 1203, 1332, 1467, 1603
BstF5I GGATG 3 cut(s) 719, 1711, 1770
BstFNI CGCG 2 cut(s) 418, 1784
BstHHI GCGC 1 cut(s) 418
BstKTI GATC 7 cut(s) 306, 868, 1146, 1225, 1249, 1312, 1801
BstMAI GTCTC 2 cut(s) 578, 1467
BstMBI GATC 7 cut(s) 303, 865, 1143, 1222, 1246, 1309, 1798
BstMWI GCNNNNNNNGC 3 cut(s) 193, 415, 1577
BstNI CCWGG 1 cut(s) 33
BstNSI RCATGY 3 cut(s) 290, 447, 1651
BstPAI GACNNNNGTC 1 cut(s) 1104
BstSCI CCNGG 3 cut(s) 31, 927, 928
BstSFI CTRYAG 2 cut(s) 168, 1635
BstUI CGCG 2 cut(s) 418, 1784
BstV1I GCAGC 7 cut(s) 40, 993, 1135, 1328, 1528, 1621, 1649
BstV2I GAAGAC 4 cut(s) 156, 242, 327, 1367
BstX2I RGATCY 3 cut(s) 865, 1309, 1798
BstXI CCANNNNNNTGG 1 cut(s) 793
BstYI RGATCY 3 cut(s) 865, 1309, 1798
Bsu15I ATCGAT 1 cut(s) 624
Bsu36I CCTNAGG 2 cut(s) 1467, 1603
BsuRI GGCC 4 cut(s) 20, 36, 926, 1171
BsuTUI ATCGAT 1 cut(s) 624
BtrI CACGTC 1 cut(s) 248
BtsCI GGATG 3 cut(s) 719, 1711, 1770
BtsI GCAGTG 1 cut(s) 902
BtsIMutI CAGTG 3 cut(s) 902, 1275, 1620
BveI ACCTGC 1 cut(s) 1585
Cac8I GCNNGC 1 cut(s) 1642
CaiI CAGNNNCTG 2 cut(s) 157, 1319
CfoI GCGC 1 cut(s) 418
Cfr10I RCCGGY 1 cut(s) 1640
Cfr13I GGNCC 3 cut(s) 35, 695, 925
Cfr9I CCCGGG 1 cut(s) 928
ClaI ATCGAT 1 cut(s) 624
CseI GACGC 2 cut(s) 1618, 1790
Csp6I GTAC 3 cut(s) 173, 1282, 1445
CspCI CAANNNNNGTGG 2 cut(s) 465, 500
CviAII CATG 6 cut(s) 287, 301, 444, 964, 1427, 1648
CviQI GTAC 3 cut(s) 173, 1282, 1445
DdeI CTNAG 7 cut(s) 84, 405, 537, 1203, 1332, 1467, 1603
DpnI GATC 7 cut(s) 305, 867, 1145, 1224, 1248, 1311, 1800
DpnII GATC 7 cut(s) 303, 865, 1143, 1222, 1246, 1309, 1798
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
EciI GGCGGA 1 cut(s) 1011
Eco130I CCWWGG 3 cut(s) 232, 1293, 1654
Eco47I GGWCC 1 cut(s) 695
Eco57I CTGAAG 3 cut(s) 41, 134, 941
Eco81I CCTNAGG 2 cut(s) 1467, 1603
Eco88I CYCGRG 1 cut(s) 928
EcoO109I RGGNCCY 1 cut(s) 925
EcoRI GAATTC 1 cut(s) 501
EcoRII CCWGG 1 cut(s) 31
EcoT14I CCWWGG 3 cut(s) 232, 1293, 1654
ErhI CCWWGG 3 cut(s) 232, 1293, 1654
FaeI CATG 6 cut(s) 290, 304, 447, 967, 1430, 1651
FaqI GGGAC 3 cut(s) 1092, 1621, 1636
FatI CATG 6 cut(s) 286, 300, 443, 963, 1426, 1647
Fnu4HI GCNGC 7 cut(s) 29, 1007, 1124, 1317, 1542, 1635, 1638
FokI GGATG 3 cut(s) 706, 1718, 1777
Fsp4HI GCNGC 7 cut(s) 29, 1007, 1124, 1317, 1542, 1635, 1638
FspBI CTAG 1 cut(s) 1389
GlaI GCGC 1 cut(s) 417
GluI GCNGC 7 cut(s) 29, 1007, 1124, 1317, 1542, 1635, 1638
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 4 cut(s) 20, 36, 926, 1171
HapII CCGG 3 cut(s) 723, 929, 1641
HgaI GACGC 2 cut(s) 1618, 1790
HhaI GCGC 1 cut(s) 418
Hin1I GRCGYC 2 cut(s) 880, 1610
Hin1II CATG 6 cut(s) 290, 304, 447, 967, 1430, 1651
Hin6I GCGC 1 cut(s) 416
HinP1I GCGC 1 cut(s) 416
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HindIII AAGCTT 1 cut(s) 664
HinfI GANTC 4 cut(s) 115, 1018, 1228, 1529
HpaII CCGG 3 cut(s) 723, 929, 1641
HphI GGTGA 4 cut(s) 1153, 1331, 1607, 1787
Hpy166II GTNNAC 5 cut(s) 339, 383, 497, 656, 842
Hpy188III TCNNGA 6 cut(s) 13, 152, 254, 1413, 1466, 1715
Hpy8I GTNNAC 5 cut(s) 339, 383, 497, 656, 842
Hpy99I CGWCG 2 cut(s) 465, 882
HpyCH4III ACNGT 4 cut(s) 64, 177, 1103, 1595
HpyCH4IV ACGT 7 cut(s) 247, 258, 360, 880, 1069, 1255, 1513
HpyCH4V TGCA 9 cut(s) 28, 170, 196, 354, 447, 895, 1544, 1580, 1637
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 415, 1577
HpyF3I CTNAG 7 cut(s) 84, 405, 537, 1203, 1332, 1467, 1603
HpySE526I ACGT 7 cut(s) 247, 258, 360, 880, 1069, 1255, 1513
Hsp92I GRCGYC 2 cut(s) 880, 1610
Hsp92II CATG 6 cut(s) 290, 304, 447, 967, 1430, 1651
HspAI GCGC 1 cut(s) 416
KroI GCCGGC 1 cut(s) 1640
KroNI GCCGGC 1 cut(s) 1642
Kzo9I GATC 7 cut(s) 303, 865, 1143, 1222, 1246, 1309, 1798
LmnI GCTCC 5 cut(s) 184, 991, 1154, 1471, 1700
Lsp1109I GCAGC 7 cut(s) 40, 993, 1135, 1328, 1528, 1621, 1649
LweI GCATC 5 cut(s) 205, 772, 1690, 1729, 1755
MaeI CTAG 1 cut(s) 1389
MaeII ACGT 7 cut(s) 247, 258, 360, 880, 1069, 1255, 1513
MaeIII GTNAC 3 cut(s) 671, 881, 1097
MalI GATC 7 cut(s) 305, 867, 1145, 1224, 1248, 1311, 1800
MbiI CCGCTC 1 cut(s) 1474
MboI GATC 7 cut(s) 303, 865, 1143, 1222, 1246, 1309, 1798
MfeI CAATTG 2 cut(s) 119, 210
MflI RGATCY 3 cut(s) 865, 1309, 1798
MlyI GAGTC 1 cut(s) 1012
MmeI TCCRAC 3 cut(s) 297, 901, 1710
MroNI GCCGGC 1 cut(s) 1640
MroXI GAANNNNTTC 1 cut(s) 1267
MseI TTAA 8 cut(s) 326, 552, 570, 686, 728, 764, 1044, 1241
MslI CAYNNNNRTG 1 cut(s) 890
MspA1I CMGCKG 4 cut(s) 313, 548, 1319, 1450
MspI CCGG 3 cut(s) 723, 929, 1641
MspR9I CCNGG 3 cut(s) 33, 929, 930
MunI CAATTG 2 cut(s) 119, 210
Mva1269I GAATGC 2 cut(s) 1240, 1573
MvaI CCWGG 1 cut(s) 33
MvnI CGCG 2 cut(s) 418, 1784
MwoI GCNNNNNNNGC 3 cut(s) 193, 415, 1577
NaeI GCCGGC 1 cut(s) 1642
NciI CCSGG 2 cut(s) 929, 930
NdeII GATC 7 cut(s) 303, 865, 1143, 1222, 1246, 1309, 1798
NgoMIV GCCGGC 1 cut(s) 1640
NlaIII CATG 6 cut(s) 290, 304, 447, 967, 1430, 1651
NlaIV GGNNCC 5 cut(s) 867, 927, 1156, 1311, 1800
NmeAIII GCCGAG 2 cut(s) 1197, 1768
NmuCI GTSAC 2 cut(s) 881, 1097
NspI RCATGY 3 cut(s) 290, 447, 1651
NspV TTCGAA 1 cut(s) 1261
PaqCI CACCTGC 1 cut(s) 1585
PciI ACATGT 1 cut(s) 1647
PctI GAATGC 2 cut(s) 1240, 1573
PdiI GCCGGC 1 cut(s) 1642
PdmI GAANNNNTTC 1 cut(s) 1267
PfeI GAWTC 3 cut(s) 115, 1228, 1529
PflMI CCANNNNNTGG 1 cut(s) 1319
PkrI GCNGC 7 cut(s) 30, 1008, 1125, 1318, 1543, 1636, 1639
PleI GAGTC 1 cut(s) 1012
PpsI GAGTC 1 cut(s) 1012
PscI ACATGT 1 cut(s) 1647
PshAI GACNNNNGTC 1 cut(s) 1104
Psp6I CCWGG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 5 cut(s) 867, 927, 1156, 1311, 1800
PspPI GGNCC 3 cut(s) 35, 695, 925
PstI CTGCAG 2 cut(s) 172, 1639
PstNI CAGNNNCTG 2 cut(s) 157, 1319
PsuI RGATCY 3 cut(s) 865, 1309, 1798
PvuII CAGCTG 3 cut(s) 313, 548, 1319
RsaI GTAC 3 cut(s) 174, 1283, 1446
RsaNI GTAC 3 cut(s) 173, 1282, 1445
RseI CAYNNNNRTG 1 cut(s) 890
SaqAI TTAA 8 cut(s) 326, 552, 570, 686, 728, 764, 1044, 1241
SatI GCNGC 7 cut(s) 29, 1007, 1124, 1317, 1542, 1635, 1638
Sau3AI GATC 7 cut(s) 303, 865, 1143, 1222, 1246, 1309, 1798
Sau96I GGNCC 3 cut(s) 35, 695, 925
SchI GAGTC 1 cut(s) 1012
ScrFI CCNGG 3 cut(s) 33, 929, 930
SfaNI GCATC 5 cut(s) 205, 772, 1690, 1729, 1755
SfcI CTRYAG 2 cut(s) 168, 1635
SfuI TTCGAA 1 cut(s) 1261
SinI GGWCC 1 cut(s) 695
SmaI CCCGGG 1 cut(s) 930
SmiMI CAYNNNNRTG 1 cut(s) 890
SmlI CTYRAG 1 cut(s) 1010
SmoI CTYRAG 1 cut(s) 1010
SsiI CCGC 5 cut(s) 1022, 1448, 1474, 1703, 1784
SspMI CTAG 1 cut(s) 1389
StyD4I CCNGG 3 cut(s) 31, 927, 928
StyI CCWWGG 3 cut(s) 232, 1293, 1654
TaaI ACNGT 4 cut(s) 64, 177, 1103, 1595
TaiI ACGT 7 cut(s) 250, 261, 363, 883, 1072, 1258, 1516
TaqI TCGA 7 cut(s) 291, 624, 912, 1261, 1378, 1414, 1749
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 3 cut(s) 115, 1228, 1529
Tru1I TTAA 8 cut(s) 326, 552, 570, 686, 728, 764, 1044, 1241
Tru9I TTAA 8 cut(s) 326, 552, 570, 686, 728, 764, 1044, 1241
TscAI CASTG 3 cut(s) 902, 1282, 1627
TseFI GTSAC 2 cut(s) 881, 1097
TseI GCWGC 7 cut(s) 28, 1006, 1123, 1316, 1541, 1634, 1637
Tsp45I GTSAC 2 cut(s) 881, 1097
TspDTI ATGAA 3 cut(s) 786, 980, 1692
TspMI CCCGGG 1 cut(s) 928
TspRI CASTG 3 cut(s) 902, 1282, 1627
Van91I CCANNNNNTGG 1 cut(s) 1319
VpaK11BI GGWCC 1 cut(s) 695
XapI RAATTY 5 cut(s) 501, 615, 713, 811, 1037
XceI RCATGY 3 cut(s) 290, 447, 1651
XmaI CCCGGG 1 cut(s) 928
XmnI GAANNNNTTC 1 cut(s) 1267
XspI CTAG 1 cut(s) 1389
ZraI GACGTC 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.