RLG00000030039

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006099
Physical Location & Seq
Reverse (-)
53758096 .. 53768999
10904 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030039

Sequence Viewer

Length: 2259 bp
ATGAACACCGCGAAAAGTGCGAGATTCGACTGGAGGATCCGAACTCCAACGACCTCCATTGAGACTGTGCTCGACTCATCGAGCTACTTTGTCCTTAAGAATGAGGACAACACCGGTAAGCACGCCTTCATCAGCCTCGGGTTTGCCGAGCGCAACGAGGAGTTCTATTTCAACGCCCGCTCTCCGATCATGACGAGTATGTCAAGAGACGAAGAGGAGTCGTACGACGTCGGATCAACCGGAACGATCTGGAGTGATCCGATTACCGGCGAAAGTCTGGGTAAAAAAAGACGAGGCTTAAGAACCGCGGAGAAGAAACCCATTCCATTCGACCCCCTCCTAAACCTCCACACTGCCCTCGCCGGTACACCCATCTCCGCCCACCAAGACGGTGCGTTTCTTCTGGTTGAGCTTTTCAACATGGAAGTGTTCAGAACTGCGATACTGCAACCAGGCCCTCCTGAAACCTTTGCATTACAGACGGTTCAGCAAGTTATAAAGCCTCAGAAAGGGACCAAGTTAGTGCAAGATGAGAATCAACTGTTGGAAAATATCCTCCGGACATTGCTACAGGAACTAGTGTCTTCTGCAGTGCAGTCTGGGGAGCAGATAATGCAGTATGGTCAATCAATTGATGATGGTGAAGCTACAAGAGGACATATTCCACGTCTTCTAGATGTCGTGCTGTATCTTTGTGAGAAAGAGCACGTGGAAGGTGGGATGATATTTCAGCTATTGGAAGACTTGACAGAGATGTCTACGATGAGAAACTGCAAGGACGTTTTCGGTTATATAGAGAGTAAGCAAGATATATTAGGAAAGCAAGAGCTTTTTGCCAGAGGAAAACTTGTGATGTTGAGAACATGCAATCAACTTCTTCGTCGTCTATCAAAGGCAAATGACGTGGTTTTTTGCGGACGAATTCTAATGTTTCTAGCACACTTTTTTCCGTTGTCTGAGCGCTCAGCTGTGAATATCAAAGGAGTTTTTAACACATCTAATGAAACGAAGTATGAGAAAGATGCCCCTGATGGAATATCCATCGATTTCAACTTCTACAAGACCTTCTGGAGCTTACAGGAACACTTTTGTAACCCTCCTTCCTTAACTGTTGCTCCTACAAAGTGGCAGAAATTTACATCCAGTTTAATGGTTGTCCTAAATACCTTTGAAGCTCAACCATTAAGTGATGAAGAGGGAGATGCCAATAATTTGGAGGAGTCAGCAAATTTCAGCATAAAGTATCTTACAAGTAGTAAGCTGATGGGTTTGGAGTTAAAGGATCCGAGTTTCCGACGACATATTCTTGTGCAGTGCCTCATATTGTTTGATTATCTGAAGGCCCCAGGAAAAAGTGAAAAAGATTTGCCTTCCGAAAGCATGAAAGAAGAAATAAATTCTTATGAGCAGCACGTGAAGAAACTGCTTAAAATGACTCCACCAAAGGGGGAAAGTTTTCTTAATAAAATTGAGCATATATTAGAACGTGAAAAGAATTGGGTATGGTGGAAACGTGATGGTTGCCCGCCATTTGAGAAGCAGCCAATAGAGAAGAAAACGGTTCAAGATGGAGCCAAAAAGCGAAAGCCGAGGTGGAGATTGGGAAATAAAGAACTCTCCCAGTTGTGGAAGTGGGCAGATCAGAATCCGAATGCTTTAACTGATCCTCAACGTCTTCAGACCCCTGCCATCACTGAGTATTGGAAACCCTTGGCTGAGGACATGGATCCAGCAGCTGGAATAGAAGCTGAATATCACCACAAAAATAACCGAGTTTACTGCTGGAAAGGTCTTCGATTTTCAGCTAGACAAGACTTGGAAGGATTTTCTAAATTTACTGAATATGGTATTGAAGGAGTGGTACCTCTAGAACTTCTGCCTCCTGAAGAACGTGCTAAATACACTCCTAAACCAAATGACAGGTCCAAACGTGCTAAAAAGGAAGATACAAAGGGTGCTGTGCATCAAGTAGAAGAAAATCAGATGGCAACAGCTGCAACTGAGGTAGATGGTGAAGGACTCAGAACTGATGTTGGGGCCTTTGTGGCTCCATTGGACACTGATAACACCGCCACGGTTGGCAATACGTCCCAAGGTAGCAGTCCAATGGCAGATGAACATCAGAAGCAAAGTTCTGATACTGATGGTGGTCAAGAGGCAGGGCAGTTGGAAGACGATGCTGAGGTGGATGCCGAGGGGGATGCCGGGATGATTGATGGAGAGATGGAACCGGAGGTTGATTTAGATGCAGTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0023052 GO:0030422 GO:0031047 GO:0031050 GO:0031503 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035556 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071369 GO:0071407 GO:0071426 GO:0071427 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0098542 GO:1901360 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
ko03013 ko03040 map03013 map03040
Pfam Domains
Protein Families
efThoc1

Protein Analysis

753

Amino Acids

84.96

Weight (kDa)

5.16

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 15 - 60 4.3e-11 Protein of unknown function (DUF1681)
efThoc1 PF11957 223 - 641 2.2e-98 THO complex subunit 1 transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
AasI GACNNNNNNGTC 2 cut(s) 199, 754
AatII GACGTC 1 cut(s) 231
Acc65I GGTACC 1 cut(s) 1861
AccB1I GGYRCC 1 cut(s) 1861
AccB7I CCANNNNNTGG 1 cut(s) 1736
AccBSI CCGCTC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 758
AccII CGCG 2 cut(s) 11, 308
AccIII TCCGGA 1 cut(s) 558
AciI CCGC 8 cut(s) 9, 178, 306, 308, 378, 915, 1526, 2070
AclWI GGATC 9 cut(s) 31, 44, 241, 251, 1277, 1290, 1658, 1721, 1734
AcsI RAATTY 5 cut(s) 921, 1133, 1228, 1396, 1832
AcuI CTGAAG 3 cut(s) 1358, 1661, 1905
AcvI CACGTG 2 cut(s) 709, 1414
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 3 cut(s) 224, 367, 1863
AfeI AGCGCT 1 cut(s) 962
AfiI CCNNNNNNNGG 5 cut(s) 266, 509, 1445, 1626, 1736
AflII CTTAAG 2 cut(s) 95, 298
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 6 cut(s) 172, 418, 1051, 1172, 1565, 1853
AhlI ACTAGT 1 cut(s) 577
AjiI CACGTC 2 cut(s) 668, 904
AjnI CCWGG 2 cut(s) 451, 1345
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
Alw21I GWGCWC 2 cut(s) 72, 708
Alw26I GTCTC 2 cut(s) 56, 201
AlwI GGATC 9 cut(s) 31, 44, 241, 251, 1277, 1290, 1658, 1721, 1734
AlwNI CAGNNNCTG 1 cut(s) 1736
Ama87I CYCGRG 1 cut(s) 137
Aor13HI TCCGGA 1 cut(s) 558
Aor51HI AGCGCT 1 cut(s) 962
AoxI GGCC 3 cut(s) 454, 1341, 2037
ApeKI GCWGC 4 cut(s) 1408, 1540, 1733, 1994
ApoI RAATTY 5 cut(s) 921, 1133, 1228, 1396, 1832
ArsI GACNNNNNNTTYG 2 cut(s) 1907, 1939
AsiGI ACCGGT 1 cut(s) 113
Asp700I GAANNNNTTC 1 cut(s) 1455
Asp718I GGTACC 1 cut(s) 1861
AspLEI GCGC 2 cut(s) 153, 963
AspS9I GGNCC 5 cut(s) 455, 513, 1342, 1923, 2037
AsuC2I CCSGG 1 cut(s) 2206
AsuHPI GGTGA 3 cut(s) 653, 1748, 2024
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 2 cut(s) 513, 1923
BamHI GGATCC 3 cut(s) 36, 1282, 1726
BanI GGYRCC 1 cut(s) 1861
BarI GAAGNNNNNNTAC 4 cut(s) 1084, 1116, 1845, 1877
BbrPI CACGTG 2 cut(s) 709, 1414
BbsI GAAGAC 6 cut(s) 576, 662, 747, 1667, 1784, 2178
Bbv12I GWGCWC 2 cut(s) 72, 708
BbvCI CCTCAGC 2 cut(s) 1716, 2181
BbvI GCAGC 4 cut(s) 1420, 1552, 1745, 1981
BcgI CGANNNNNNTGC 4 cut(s) 1761, 1795, 2183, 2217
BciT130I CCWGG 2 cut(s) 453, 1347
BcnI CCSGG 1 cut(s) 2206
BcoDI GTCTC 2 cut(s) 56, 201
BcuI ACTAGT 1 cut(s) 577
BfaI CTAG 5 cut(s) 578, 674, 935, 1806, 1868
BfmI CTRYAG 2 cut(s) 569, 588
BfoI RGCGCY 1 cut(s) 964
BfrI CTTAAG 2 cut(s) 95, 298
BglI GCCNNNNNGGC 1 cut(s) 2045
BisI GCNGC 4 cut(s) 1409, 1541, 1734, 1995
BlpI GCTNAGC 1 cut(s) 964
BlsI GCNGC 4 cut(s) 1410, 1542, 1735, 1996
Bme1390I CCNGG 3 cut(s) 453, 1347, 2206
Bme18I GGWCC 2 cut(s) 513, 1923
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 2 cut(s) 668, 904
BmgT120I GGNCC 5 cut(s) 455, 513, 1342, 1923, 2037
BmrFI CCNGG 3 cut(s) 453, 1347, 2206
BmrI ACTGGG 1 cut(s) 1615
BmsI GCATC 7 cut(s) 1012, 1192, 1972, 2167, 2179, 2191, 2236
BmuI ACTGGG 1 cut(s) 1615
BpiI GAAGAC 6 cut(s) 576, 662, 747, 1667, 1784, 2178
BpmI CTGGAG 3 cut(s) 52, 271, 1090
Bpu10I CCTNAGC 2 cut(s) 1716, 2181
Bpu1102I GCTNAGC 1 cut(s) 964
BpuMI CCSGG 1 cut(s) 2206
Bsa29I ATCGAT 1 cut(s) 1044
BsaAI YACGTR 2 cut(s) 709, 1414
BsaBI GATNNNNATC 3 cut(s) 534, 1644, 2118
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 8 cut(s) 136, 306, 1345, 1589, 1710, 2073, 2092, 2193
BsaWI WCCGGW 4 cut(s) 113, 239, 558, 2230
BsaXI ACNNNNNCTCC 2 cut(s) 1099, 1129
Bsc4I CCNNNNNNNGG 5 cut(s) 266, 509, 1445, 1626, 1736
Bse118I RCCGGY 3 cut(s) 113, 266, 362
Bse1I ACTGG 3 cut(s) 35, 1143, 1621
Bse3DI GCAATG 1 cut(s) 563
Bse8I GATNNNNATC 3 cut(s) 534, 1644, 2118
BseAI TCCGGA 1 cut(s) 558
BseBI CCWGG 2 cut(s) 453, 1347
BseCI ATCGAT 1 cut(s) 1044
BseDI CCNNGG 8 cut(s) 136, 306, 1345, 1589, 1710, 2073, 2092, 2193
BseGI GGATG 5 cut(s) 726, 1139, 2194, 2206, 2214
BseJI GATNNNNATC 3 cut(s) 534, 1644, 2118
BseLI CCNNNNNNNGG 5 cut(s) 266, 509, 1445, 1626, 1736
BseMI GCAATG 1 cut(s) 563
BseMII CTCAG 8 cut(s) 518, 948, 978, 1686, 1707, 1992, 2035, 2172
BseNI ACTGG 3 cut(s) 35, 1143, 1621
BseRI GAGGAG 3 cut(s) 173, 230, 1232
BseXI GCAGC 4 cut(s) 1420, 1552, 1745, 1981
BsgI GTGCAG 2 cut(s) 614, 1331
Bsh1236I CGCG 2 cut(s) 11, 308
BshFI GGCC 3 cut(s) 456, 1343, 2039
BshNI GGYRCC 1 cut(s) 1861
BshTI ACCGGT 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 1044
BsiHKAI GWGCWC 2 cut(s) 72, 708
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 7 cut(s) 114, 240, 267, 363, 559, 2205, 2231
BsiWI CGTACG 1 cut(s) 222
BslFI GGGAC 2 cut(s) 526, 2074
BslI CCNNNNNNNGG 5 cut(s) 266, 509, 1445, 1626, 1736
BsmAI GTCTC 2 cut(s) 56, 201
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 2 cut(s) 526, 2074
BsmI GAATGC 1 cut(s) 1657
BsnI GGCC 3 cut(s) 456, 1343, 2039
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 2 cut(s) 72, 708
Bsp13I TCCGGA 1 cut(s) 558
Bsp143I GATC 9 cut(s) 36, 186, 233, 246, 256, 1282, 1639, 1663, 1726
Bsp1720I GCTNAGC 1 cut(s) 964
BspACI CCGC 8 cut(s) 9, 178, 306, 308, 378, 915, 1526, 2070
BspANI GGCC 3 cut(s) 456, 1343, 2039
BspCNI CTCAG 8 cut(s) 517, 949, 977, 1687, 1708, 1993, 2034, 2173
BspDI ATCGAT 1 cut(s) 1044
BspEI TCCGGA 1 cut(s) 558
BspFNI CGCG 2 cut(s) 11, 308
BspHI TCATGA 1 cut(s) 189
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 9 cut(s) 31, 44, 241, 251, 1277, 1290, 1658, 1721, 1734
BspT107I GGYRCC 1 cut(s) 1861
BspTI CTTAAG 2 cut(s) 95, 298
BsrBI CCGCTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 563
BsrFI RCCGGY 3 cut(s) 113, 266, 362
BsrI ACTGG 3 cut(s) 35, 1143, 1621
BssAI RCCGGY 3 cut(s) 113, 266, 362
BssECI CCNNGG 8 cut(s) 136, 306, 1345, 1589, 1710, 2073, 2092, 2193
BssMI GATC 9 cut(s) 36, 186, 233, 246, 256, 1282, 1639, 1663, 1726
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 1710, 2092
Bst2UI CCWGG 2 cut(s) 453, 1347
Bst4CI ACNGT 7 cut(s) 67, 392, 484, 543, 1111, 1561, 2077
Bst6I CTCTTC 2 cut(s) 207, 1188
BstACI GRCGYC 1 cut(s) 228
BstAFI CTTAAG 2 cut(s) 95, 298
BstAPI GCANNNNNTGC 2 cut(s) 613, 1994
BstBAI YACGTR 2 cut(s) 709, 1414
BstC8I GCNNGC 3 cut(s) 123, 178, 1526
BstDEI CTNAG 8 cut(s) 504, 957, 964, 1695, 1716, 2001, 2021, 2181
BstDSI CCRYGG 2 cut(s) 306, 2073
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 5 cut(s) 726, 1139, 2194, 2206, 2214
BstFNI CGCG 2 cut(s) 11, 308
BstH2I RGCGCY 1 cut(s) 964
BstHHI GCGC 2 cut(s) 153, 963
BstKTI GATC 9 cut(s) 39, 189, 236, 249, 259, 1285, 1642, 1666, 1729
BstMAI GTCTC 2 cut(s) 56, 201
BstMBI GATC 9 cut(s) 36, 186, 233, 246, 256, 1282, 1639, 1663, 1726
BstMWI GCNNNNNNNGC 4 cut(s) 17, 613, 1994, 2045
BstNI CCWGG 2 cut(s) 453, 1347
BstNSI RCATGY 1 cut(s) 867
BstSCI CCNGG 3 cut(s) 451, 1345, 2204
BstSFI CTRYAG 2 cut(s) 569, 588
BstUI CGCG 2 cut(s) 11, 308
BstV1I GCAGC 4 cut(s) 1420, 1552, 1745, 1981
BstV2I GAAGAC 6 cut(s) 576, 662, 747, 1667, 1784, 2178
BstX2I RGATCY 3 cut(s) 36, 1282, 1726
BstXI CCANNNNNNTGG 2 cut(s) 1150, 1213
BstYI RGATCY 3 cut(s) 36, 1282, 1726
Bsu15I ATCGAT 1 cut(s) 1044
BsuRI GGCC 3 cut(s) 456, 1343, 2039
BsuTUI ATCGAT 1 cut(s) 1044
BtgI CCRYGG 2 cut(s) 306, 2073
BtrI CACGTC 2 cut(s) 668, 904
BtsCI GGATG 5 cut(s) 726, 1139, 2194, 2206, 2214
BtsI GCAGTG 3 cut(s) 351, 597, 1319
BtsIMutI CAGTG 5 cut(s) 351, 597, 1319, 1692, 2058
Cac8I GCNNGC 3 cut(s) 123, 178, 1526
CaiI CAGNNNCTG 1 cut(s) 1736
CciI TCATGA 1 cut(s) 189
CfoI GCGC 2 cut(s) 153, 963
Cfr10I RCCGGY 3 cut(s) 113, 266, 362
Cfr13I GGNCC 5 cut(s) 455, 513, 1342, 1923, 2037
Cfr42I CCGCGG 1 cut(s) 309
ClaI ATCGAT 1 cut(s) 1044
Csp6I GTAC 3 cut(s) 223, 366, 1862
CspAI ACCGGT 1 cut(s) 113
CspCI CAANNNNNGTGG 2 cut(s) 885, 920
CviAII CATG 5 cut(s) 190, 421, 864, 1381, 1723
CviQI GTAC 3 cut(s) 223, 366, 1862
DdeI CTNAG 8 cut(s) 504, 957, 964, 1695, 1716, 2001, 2021, 2181
DpnI GATC 9 cut(s) 38, 188, 235, 248, 258, 1284, 1641, 1665, 1728
DpnII GATC 9 cut(s) 36, 186, 233, 246, 256, 1282, 1639, 1663, 1726
DrdI GACNNNNNNGTC 2 cut(s) 199, 754
DseDI GACNNNNNNGTC 2 cut(s) 199, 754
Eam1104I CTCTTC 2 cut(s) 207, 1188
EarI CTCTTC 2 cut(s) 207, 1188
EciI GGCGGA 1 cut(s) 367
Eco130I CCWWGG 2 cut(s) 1710, 2092
Eco47I GGWCC 2 cut(s) 513, 1923
Eco47III AGCGCT 1 cut(s) 962
Eco57I CTGAAG 3 cut(s) 1358, 1661, 1905
Eco72I CACGTG 2 cut(s) 709, 1414
Eco88I CYCGRG 1 cut(s) 137
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 3 cut(s) 455, 1342, 2037
EcoRI GAATTC 1 cut(s) 921
EcoRII CCWGG 2 cut(s) 451, 1345
EcoT14I CCWWGG 2 cut(s) 1710, 2092
ErhI CCWWGG 2 cut(s) 1710, 2092
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 5 cut(s) 193, 424, 867, 1384, 1726
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 2 cut(s) 526, 2074
FatI CATG 5 cut(s) 189, 420, 863, 1380, 1722
FauI CCCGC 2 cut(s) 185, 1533
FblI GTMKAC 1 cut(s) 758
Fnu4HI GCNGC 4 cut(s) 1409, 1541, 1734, 1995
FokI GGATG 5 cut(s) 733, 1126, 2201, 2213, 2221
Fsp4HI GCNGC 4 cut(s) 1409, 1541, 1734, 1995
FspBI CTAG 5 cut(s) 578, 674, 935, 1806, 1868
GlaI GCGC 2 cut(s) 152, 962
GluI GCNGC 4 cut(s) 1409, 1541, 1734, 1995
GsuI CTGGAG 3 cut(s) 52, 271, 1090
HaeII RGCGCY 1 cut(s) 964
HaeIII GGCC 3 cut(s) 456, 1343, 2039
HapII CCGG 7 cut(s) 114, 240, 267, 363, 559, 2205, 2231
HhaI GCGC 2 cut(s) 153, 963
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 5 cut(s) 193, 424, 867, 1384, 1726
Hin6I GCGC 2 cut(s) 151, 961
HinP1I GCGC 2 cut(s) 151, 961
HinfI GANTC 8 cut(s) 24, 74, 218, 535, 1220, 1435, 1645, 2019
HpaII CCGG 7 cut(s) 114, 240, 267, 363, 559, 2205, 2231
HphI GGTGA 3 cut(s) 653, 1748, 2024
Hpy166II GTNNAC 3 cut(s) 368, 759, 1777
Hpy8I GTNNAC 3 cut(s) 368, 759, 1777
Hpy99I CGWCG 4 cut(s) 230, 233, 885, 1299
HpyAV CCTTC 9 cut(s) 136, 707, 1075, 1110, 1333, 1380, 1814, 1847, 2009
HpyCH4III ACNGT 7 cut(s) 67, 392, 484, 543, 1111, 1561, 2077
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 613, 1994, 2045
HpyF3I CTNAG 8 cut(s) 504, 957, 964, 1695, 1716, 2001, 2021, 2181
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 5 cut(s) 193, 424, 867, 1384, 1726
HspAI GCGC 2 cut(s) 151, 961
Kpn2I TCCGGA 1 cut(s) 558
KpnI GGTACC 1 cut(s) 1865
KspI CCGCGG 1 cut(s) 309
Kzo9I GATC 9 cut(s) 36, 186, 233, 246, 256, 1282, 1639, 1663, 1726
LmnI GCTCC 5 cut(s) 604, 1071, 1120, 1571, 2053
Lsp1109I GCAGC 4 cut(s) 1420, 1552, 1745, 1981
LweI GCATC 7 cut(s) 1012, 1192, 1972, 2167, 2179, 2191, 2236
MaeI CTAG 5 cut(s) 578, 674, 935, 1806, 1868
MaeIII GTNAC 1 cut(s) 1091
MalI GATC 9 cut(s) 38, 188, 235, 248, 258, 1284, 1641, 1665, 1728
MbiI CCGCTC 1 cut(s) 180
MboI GATC 9 cut(s) 36, 186, 233, 246, 256, 1282, 1639, 1663, 1726
MfeI CAATTG 1 cut(s) 630
MflI RGATCY 3 cut(s) 36, 1282, 1726
MhlI GDGCHC 2 cut(s) 72, 708
MluCI AATT 9 cut(s) 630, 921, 1133, 1210, 1228, 1396, 1467, 1495, 1832
MlyI GAGTC 5 cut(s) 68, 227, 1229, 1429, 2013
MmeI TCCRAC 5 cut(s) 71, 211, 525, 1318, 2148
MroI TCCGGA 1 cut(s) 558
MroXI GAANNNNTTC 1 cut(s) 1455
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 4 cut(s) 308, 968, 1736, 1994
MspCI CTTAAG 2 cut(s) 95, 298
MspI CCGG 7 cut(s) 114, 240, 267, 363, 559, 2205, 2231
MspR9I CCNGG 3 cut(s) 453, 1347, 2206
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 1 cut(s) 1657
MvaI CCWGG 2 cut(s) 453, 1347
MvnI CGCG 2 cut(s) 11, 308
MwoI GCNNNNNNNGC 4 cut(s) 17, 613, 1994, 2045
NciI CCSGG 1 cut(s) 2206
NdeII GATC 9 cut(s) 36, 186, 233, 246, 256, 1282, 1639, 1663, 1726
NlaIII CATG 5 cut(s) 193, 424, 867, 1384, 1726
NmeAIII GCCGAG 3 cut(s) 172, 1614, 2218
NspI RCATGY 1 cut(s) 867
PagI TCATGA 1 cut(s) 189
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 1657
PdmI GAANNNNTTC 1 cut(s) 1455
PfeI GAWTC 3 cut(s) 24, 535, 1645
Pfl23II CGTACG 1 cut(s) 222
PflMI CCANNNNNTGG 1 cut(s) 1736
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 4 cut(s) 1410, 1542, 1735, 1996
PleI GAGTC 5 cut(s) 68, 226, 1228, 1429, 2013
PmaCI CACGTG 2 cut(s) 709, 1414
PmlI CACGTG 2 cut(s) 709, 1414
PpsI GAGTC 5 cut(s) 68, 226, 1228, 1429, 2013
Ppu21I YACGTR 2 cut(s) 709, 1414
PsiI TTATAA 1 cut(s) 497
Psp6I CCWGG 2 cut(s) 451, 1345
PspCI CACGTG 2 cut(s) 709, 1414
PspGI CCWGG 2 cut(s) 451, 1345
PspLI CGTACG 1 cut(s) 222
PspPI GGNCC 5 cut(s) 455, 513, 1342, 1923, 2037
PstI CTGCAG 1 cut(s) 592
PstNI CAGNNNCTG 1 cut(s) 1736
PsuI RGATCY 3 cut(s) 36, 1282, 1726
PvuII CAGCTG 3 cut(s) 968, 1736, 1994
RsaI GTAC 3 cut(s) 224, 367, 1863
RsaNI GTAC 3 cut(s) 223, 366, 1862
RseI CAYNNNNRTG 1 cut(s) 425
SacII CCGCGG 1 cut(s) 309
SatI GCNGC 4 cut(s) 1409, 1541, 1734, 1995
Sau3AI GATC 9 cut(s) 36, 186, 233, 246, 256, 1282, 1639, 1663, 1726
Sau96I GGNCC 5 cut(s) 455, 513, 1342, 1923, 2037
SchI GAGTC 5 cut(s) 68, 227, 1229, 1429, 2013
ScrFI CCNGG 3 cut(s) 453, 1347, 2206
SduI GDGCHC 2 cut(s) 72, 708
SfaNI GCATC 7 cut(s) 1012, 1192, 1972, 2167, 2179, 2191, 2236
SfcI CTRYAG 2 cut(s) 569, 588
Sfr303I CCGCGG 1 cut(s) 309
SgrBI CCGCGG 1 cut(s) 309
SinI GGWCC 2 cut(s) 513, 1923
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 2 cut(s) 95, 298
SmoI CTYRAG 2 cut(s) 95, 298
SpeI ACTAGT 1 cut(s) 577
Sse9I AATT 9 cut(s) 630, 921, 1133, 1210, 1228, 1396, 1467, 1495, 1832
SsiI CCGC 8 cut(s) 9, 178, 306, 308, 378, 915, 1526, 2070
SspMI CTAG 5 cut(s) 578, 674, 935, 1806, 1868
StyD4I CCNGG 3 cut(s) 451, 1345, 2204
StyI CCWWGG 2 cut(s) 1710, 2092
TaaI ACNGT 7 cut(s) 67, 392, 484, 543, 1111, 1561, 2077
TaqI TCGA 6 cut(s) 27, 72, 80, 330, 1044, 1795
TasI AATT 9 cut(s) 630, 921, 1133, 1210, 1228, 1396, 1467, 1495, 1832
TfiI GAWTC 3 cut(s) 24, 535, 1645
TscAI CASTG 5 cut(s) 358, 597, 1319, 1699, 2065
TseI GCWGC 4 cut(s) 1408, 1540, 1733, 1994
TspDTI ATGAA 6 cut(s) 17, 118, 1017, 1206, 1397, 2130
TspGWI ACGGA 1 cut(s) 939
TspRI CASTG 5 cut(s) 358, 597, 1319, 1699, 2065
Van91I CCANNNNNTGG 1 cut(s) 1736
Vha464I CTTAAG 2 cut(s) 95, 298
VpaK11BI GGWCC 2 cut(s) 513, 1923
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 5 cut(s) 921, 1133, 1228, 1396, 1832
XbaI TCTAGA 2 cut(s) 673, 1867
XceI RCATGY 1 cut(s) 867
XmiI GTMKAC 1 cut(s) 758
XmnI GAANNNNTTC 1 cut(s) 1455
XspI CTAG 5 cut(s) 578, 674, 935, 1806, 1868
ZraI GACGTC 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.