Rh1BG064300

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006099
Physical Location & Seq
Forward (+)
9683161 .. 9698516
15356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG064300.1

Sequence Viewer

Length: 1419 bp
ATGGAAGTGTTCAGAACTGCGATACTGCAACCAGGACCTCCTGAAACCTTTGCATTACAGACGGTTCAGCAAGTTATAAAGCCTCAGAAAGGGACCAAGTTAGTGCAAGATGAGAATCAACTGTTGGAAAATATCCTCCGGACATTGCTACAGGAACTAGTGTCTTCTGCAGTACAGTCCGGGGAGCAGATAATGCAGTATGGTCAATCAATTGATGATGGAGAAGCTGCAAGAGGACATATTCCACGTCTTCTAGATGTCGTGCTGTATCTTTGTGAGAAAGAGCACGTGGAAGGTGGCATGATATTTCAGCTACTGGAAGACTTGACAGAGATGTCTACGATGAGAAACTGCAAAGACGTTTTCGGTTATATAGAGAGTAAGCAAGATATATTAGGAAAGCAAGAGCTTTTTGCCAGAGGAAAACTTGTGATGTTGAGAACATGCAATCAACTTCTTCGTCGTCTATCAAAGGCAAATGACGTGGTTTTTTGCGGACGAATTCTAATGTTTCTAGCACACTTTTTCCCATTGTCTGAGCGCTCAGCTGTGAATATCAAAGGAGTTTTTAACACATCTAATGAAACGAAGTATGAGAAAGATGCCCCTGATGGAATATCCATCGATTTCAACTTCTACAAGACCTTCTGGAGCTTACAGGAACACTTTTGTAACCCTCCTTCCTTAACTGTTGCTCCTACAAAGTGGCAGAAATTTTCATCCAGTTTAATGGTTGTCCTAAATACCTTTGAAGCTCAACCATTAAGTGATGAAGAGGGAGATGCCAATAATTTGGAGGAGTCAGCAAATTTCAGCATAAAGTATCTTACAAGTAGTAAGCTGATGGGTTTGGAGTTAAAGGATCCGAGTTTCCGACGACACATTCTTGTGCAGTGCCTCATATTGTTCGATTATCTGAAGGCCCCAGGAAAAAGTGAAAAAGATTTGCCTTCTGAAAGCATGAAAGAAGAAATAAATTCTTATGAGCAGCATGTGAAGAAACTGCTTAAAATGACTCCACCAAAGGGGGAAAGTTTTCTTAATAAAATTGAGCATATATTAGAACGTGAAAAGAATTGGGTATGGTGGAAACGTGATGGTTGCCCTCCATTTGAGAAGCAGCCAATAGAGAAGAAAACGGTTCAAGATGGAGCCAAAAAGCGAAAGCCGAGGTGGAGATTGGGAAATAAAGAACTCTCCCAGTTGTGGAAGTGGGCAGATCAGAATCCGAATGCTTTAACTGATCCTCAATGTCTTCAGACCCCTGCCATCTCTGAGTATTGGAAACCCTTGGCTGAGGACATGGATCCAGCAGCTGGAATAGAAGCTGAATATCACCACAAAAATAACCGAGTTTACTGCTGGAAAGGTCTTCGATTTTCAGCTAGACAAGACTTGGAAGGATTTTCTAAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0023052 GO:0030422 GO:0031047 GO:0031050 GO:0031503 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035556 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071369 GO:0071407 GO:0071426 GO:0071427 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0098542 GO:1901360 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
ko03013 ko03040 map03013 map03040
Pfam Domains
Protein Families
efThoc1

Protein Analysis

472

Amino Acids

54.36

Weight (kDa)

6.55

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
efThoc1 PF11957 83 - 472 7.4e-96 THO complex subunit 1 transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
AasI GACNNNNNNGTC 1 cut(s) 334
AccB7I CCANNNNNTGG 1 cut(s) 1316
AccI GTMKAC 1 cut(s) 338
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 1 cut(s) 495
AclWI GGATC 5 cut(s) 857, 870, 1238, 1301, 1314
AcsI RAATTY 4 cut(s) 501, 713, 808, 976
AcuI CTGAAG 2 cut(s) 938, 1241
AcvI CACGTG 1 cut(s) 289
AfaI GTAC 1 cut(s) 174
AfeI AGCGCT 1 cut(s) 542
AfiI CCNNNNNNNGG 4 cut(s) 89, 1025, 1206, 1316
AgsI TTSAA 3 cut(s) 631, 752, 1145
AhlI ACTAGT 1 cut(s) 157
AjiI CACGTC 2 cut(s) 248, 484
AjnI CCWGG 2 cut(s) 31, 925
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
Alw21I GWGCWC 1 cut(s) 288
AlwI GGATC 5 cut(s) 857, 870, 1238, 1301, 1314
AlwNI CAGNNNCTG 2 cut(s) 316, 1316
Aor13HI TCCGGA 1 cut(s) 138
Aor51HI AGCGCT 1 cut(s) 542
AoxI GGCC 1 cut(s) 921
ApeKI GCWGC 4 cut(s) 227, 988, 1120, 1313
ApoI RAATTY 4 cut(s) 501, 713, 808, 976
Asp700I GAANNNNTTC 1 cut(s) 1035
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 3 cut(s) 35, 93, 922
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 1328
AvaII GGWCC 2 cut(s) 35, 93
BamHI GGATCC 2 cut(s) 862, 1306
BarI GAAGNNNNNNTAC 2 cut(s) 664, 696
BbrPI CACGTG 1 cut(s) 289
BbsI GAAGAC 5 cut(s) 156, 242, 327, 1247, 1364
Bbv12I GWGCWC 1 cut(s) 288
BbvCI CCTCAGC 1 cut(s) 1296
BbvI GCAGC 4 cut(s) 214, 1000, 1132, 1325
BccI CCATC 7 cut(s) 212, 605, 629, 838, 1091, 1142, 1277
BcgI CGANNNNNNTGC 2 cut(s) 1341, 1375
BciT130I CCWGG 2 cut(s) 33, 927
BcnI CCSGG 1 cut(s) 181
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 4 cut(s) 158, 254, 515, 1386
BfmI CTRYAG 2 cut(s) 149, 168
BfoI RGCGCY 1 cut(s) 544
BisI GCNGC 4 cut(s) 228, 989, 1121, 1314
BlpI GCTNAGC 1 cut(s) 544
BlsI GCNGC 4 cut(s) 229, 990, 1122, 1315
Bme1390I CCNGG 3 cut(s) 33, 181, 927
Bme18I GGWCC 2 cut(s) 35, 93
BmgBI CACGTC 2 cut(s) 248, 484
BmgT120I GGNCC 3 cut(s) 35, 93, 922
BmiI GGNNCC 5 cut(s) 94, 864, 924, 1153, 1308
BmrFI CCNGG 3 cut(s) 33, 181, 927
BmrI ACTGGG 1 cut(s) 1195
BmsI GCATC 2 cut(s) 592, 772
BmuI ACTGGG 1 cut(s) 1195
BpiI GAAGAC 5 cut(s) 156, 242, 327, 1247, 1364
BpmI CTGGAG 1 cut(s) 670
Bpu10I CCTNAGC 1 cut(s) 1296
Bpu1102I GCTNAGC 1 cut(s) 544
BpuMI CCSGG 1 cut(s) 181
Bsa29I ATCGAT 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 289
BsaBI GATNNNNATC 2 cut(s) 114, 1224
BsaJI CCNNGG 4 cut(s) 180, 925, 1169, 1290
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 1025, 1206, 1316
Bse1I ACTGG 3 cut(s) 321, 723, 1201
Bse3DI GCAATG 1 cut(s) 143
Bse8I GATNNNNATC 2 cut(s) 114, 1224
BseAI TCCGGA 1 cut(s) 138
BseBI CCWGG 2 cut(s) 33, 927
BseCI ATCGAT 1 cut(s) 624
BseDI CCNNGG 4 cut(s) 180, 925, 1169, 1290
BseGI GGATG 1 cut(s) 719
BseJI GATNNNNATC 2 cut(s) 114, 1224
BseLI CCNNNNNNNGG 4 cut(s) 89, 1025, 1206, 1316
BseMI GCAATG 1 cut(s) 143
BseMII CTCAG 5 cut(s) 98, 528, 558, 1266, 1287
BseNI ACTGG 3 cut(s) 321, 723, 1201
BseRI GAGGAG 1 cut(s) 812
BseXI GCAGC 4 cut(s) 214, 1000, 1132, 1325
BsgI GTGCAG 1 cut(s) 911
BshFI GGCC 1 cut(s) 923
BshVI ATCGAT 1 cut(s) 624
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 2 cut(s) 139, 180
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 4 cut(s) 89, 1025, 1206, 1316
BsmFI GGGAC 1 cut(s) 106
BsmI GAATGC 1 cut(s) 1237
BsnI GGCC 1 cut(s) 923
Bsp1286I GDGCHC 1 cut(s) 288
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 4 cut(s) 862, 1219, 1243, 1306
Bsp1720I GCTNAGC 1 cut(s) 544
BspACI CCGC 1 cut(s) 495
BspANI GGCC 1 cut(s) 923
BspCNI CTCAG 5 cut(s) 97, 529, 557, 1267, 1288
BspDI ATCGAT 1 cut(s) 624
BspEI TCCGGA 1 cut(s) 138
BspLI GGNNCC 5 cut(s) 94, 864, 924, 1153, 1308
BspMAI CTGCAG 1 cut(s) 172
BspPI GGATC 5 cut(s) 857, 870, 1238, 1301, 1314
BsrDI GCAATG 1 cut(s) 143
BsrI ACTGG 3 cut(s) 321, 723, 1201
BssECI CCNNGG 4 cut(s) 180, 925, 1169, 1290
BssMI GATC 4 cut(s) 862, 1219, 1243, 1306
BssT1I CCWWGG 1 cut(s) 1290
Bst2UI CCWGG 2 cut(s) 33, 927
Bst4CI ACNGT 5 cut(s) 64, 123, 177, 691, 1141
Bst6I CTCTTC 1 cut(s) 768
BstAPI GCANNNNNTGC 1 cut(s) 193
BstBAI YACGTR 1 cut(s) 289
BstDEI CTNAG 5 cut(s) 84, 537, 544, 1275, 1296
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 719
BstH2I RGCGCY 1 cut(s) 544
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 4 cut(s) 865, 1222, 1246, 1309
BstMBI GATC 4 cut(s) 862, 1219, 1243, 1306
BstMWI GCNNNNNNNGC 1 cut(s) 193
BstNI CCWGG 2 cut(s) 33, 927
BstNSI RCATGY 2 cut(s) 447, 995
BstSCI CCNGG 3 cut(s) 31, 179, 925
BstSFI CTRYAG 2 cut(s) 149, 168
BstV1I GCAGC 4 cut(s) 214, 1000, 1132, 1325
BstV2I GAAGAC 5 cut(s) 156, 242, 327, 1247, 1364
BstX2I RGATCY 2 cut(s) 862, 1306
BstXI CCANNNNNNTGG 2 cut(s) 730, 793
BstYI RGATCY 2 cut(s) 862, 1306
Bsu15I ATCGAT 1 cut(s) 624
BsuRI GGCC 1 cut(s) 923
BsuTUI ATCGAT 1 cut(s) 624
BtrI CACGTC 2 cut(s) 248, 484
BtsCI GGATG 1 cut(s) 719
BtsI GCAGTG 1 cut(s) 899
BtsIMutI CAGTG 1 cut(s) 899
CaiI CAGNNNCTG 2 cut(s) 316, 1316
CfoI GCGC 1 cut(s) 543
Cfr13I GGNCC 3 cut(s) 35, 93, 922
ClaI ATCGAT 1 cut(s) 624
Csp6I GTAC 1 cut(s) 173
CspCI CAANNNNNGTGG 2 cut(s) 465, 500
CviAII CATG 5 cut(s) 301, 444, 961, 992, 1303
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 5 cut(s) 84, 537, 544, 1275, 1296
DpnI GATC 4 cut(s) 864, 1221, 1245, 1308
DpnII GATC 4 cut(s) 862, 1219, 1243, 1306
DrdI GACNNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 1 cut(s) 1290
Eco47I GGWCC 2 cut(s) 35, 93
Eco47III AGCGCT 1 cut(s) 542
Eco57I CTGAAG 2 cut(s) 938, 1241
Eco72I CACGTG 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoO109I RGGNCCY 2 cut(s) 35, 922
EcoRI GAATTC 1 cut(s) 501
EcoRII CCWGG 2 cut(s) 31, 925
EcoT14I CCWWGG 1 cut(s) 1290
ErhI CCWWGG 1 cut(s) 1290
FaeI CATG 5 cut(s) 304, 447, 964, 995, 1306
FaqI GGGAC 1 cut(s) 106
FatI CATG 5 cut(s) 300, 443, 960, 991, 1302
FblI GTMKAC 1 cut(s) 338
Fnu4HI GCNGC 4 cut(s) 228, 989, 1121, 1314
FokI GGATG 1 cut(s) 706
Fsp4HI GCNGC 4 cut(s) 228, 989, 1121, 1314
FspBI CTAG 4 cut(s) 158, 254, 515, 1386
GlaI GCGC 1 cut(s) 542
GluI GCNGC 4 cut(s) 228, 989, 1121, 1314
GsuI CTGGAG 1 cut(s) 670
HaeII RGCGCY 1 cut(s) 544
HaeIII GGCC 1 cut(s) 923
HapII CCGG 2 cut(s) 139, 180
HhaI GCGC 1 cut(s) 543
Hin1II CATG 5 cut(s) 304, 447, 964, 995, 1306
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HinfI GANTC 4 cut(s) 115, 800, 1015, 1225
HpaII CCGG 2 cut(s) 139, 180
HphI GGTGA 1 cut(s) 1328
Hpy166II GTNNAC 2 cut(s) 339, 1357
Hpy188III TCNNGA 5 cut(s) 41, 139, 254, 649, 1145
Hpy8I GTNNAC 2 cut(s) 339, 1357
Hpy99I CGWCG 2 cut(s) 465, 879
HpyAV CCTTC 6 cut(s) 287, 655, 690, 913, 960, 1394
HpyCH4III ACNGT 5 cut(s) 64, 123, 177, 691, 1141
HpyCH4IV ACGT 6 cut(s) 247, 288, 360, 483, 1066, 1093
HpyCH4V TGCA 9 cut(s) 28, 53, 106, 170, 196, 230, 354, 447, 892
HpyF10VI GCNNNNNNNGC 1 cut(s) 193
HpyF3I CTNAG 5 cut(s) 84, 537, 544, 1275, 1296
HpySE526I ACGT 6 cut(s) 247, 288, 360, 483, 1066, 1093
Hsp92II CATG 5 cut(s) 304, 447, 964, 995, 1306
HspAI GCGC 1 cut(s) 541
Kpn2I TCCGGA 1 cut(s) 138
Kzo9I GATC 4 cut(s) 862, 1219, 1243, 1306
LmnI GCTCC 4 cut(s) 184, 651, 700, 1151
Lsp1109I GCAGC 4 cut(s) 214, 1000, 1132, 1325
LweI GCATC 2 cut(s) 592, 772
MaeI CTAG 4 cut(s) 158, 254, 515, 1386
MaeII ACGT 6 cut(s) 247, 288, 360, 483, 1066, 1093
MaeIII GTNAC 1 cut(s) 671
MalI GATC 4 cut(s) 864, 1221, 1245, 1308
MboI GATC 4 cut(s) 862, 1219, 1243, 1306
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 2 cut(s) 862, 1306
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 8 cut(s) 210, 501, 713, 790, 808, 976, 1047, 1075
MlyI GAGTC 2 cut(s) 809, 1009
MmeI TCCRAC 2 cut(s) 105, 898
MroI TCCGGA 1 cut(s) 138
MroXI GAANNNNTTC 1 cut(s) 1035
MseI TTAA 8 cut(s) 570, 686, 728, 764, 857, 1008, 1041, 1238
MslI CAYNNNNRTG 1 cut(s) 887
MspA1I CMGCKG 2 cut(s) 548, 1316
MspI CCGG 2 cut(s) 139, 180
MspR9I CCNGG 3 cut(s) 33, 181, 927
MunI CAATTG 1 cut(s) 210
Mva1269I GAATGC 1 cut(s) 1237
MvaI CCWGG 2 cut(s) 33, 927
MwoI GCNNNNNNNGC 1 cut(s) 193
NciI CCSGG 1 cut(s) 181
NdeII GATC 4 cut(s) 862, 1219, 1243, 1306
NlaIII CATG 5 cut(s) 304, 447, 964, 995, 1306
NlaIV GGNNCC 5 cut(s) 94, 864, 924, 1153, 1308
NmeAIII GCCGAG 1 cut(s) 1194
NspI RCATGY 2 cut(s) 447, 995
PctI GAATGC 1 cut(s) 1237
PdmI GAANNNNTTC 1 cut(s) 1035
PfeI GAWTC 2 cut(s) 115, 1225
PflMI CCANNNNNTGG 1 cut(s) 1316
PkrI GCNGC 4 cut(s) 229, 990, 1122, 1315
PleI GAGTC 2 cut(s) 808, 1009
PmaCI CACGTG 1 cut(s) 289
PmlI CACGTG 1 cut(s) 289
PpsI GAGTC 2 cut(s) 808, 1009
Ppu21I YACGTR 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 35
PsiI TTATAA 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 31, 925
PspCI CACGTG 1 cut(s) 289
PspGI CCWGG 2 cut(s) 31, 925
PspN4I GGNNCC 5 cut(s) 94, 864, 924, 1153, 1308
PspPI GGNCC 3 cut(s) 35, 93, 922
PspPPI RGGWCCY 1 cut(s) 35
PstI CTGCAG 1 cut(s) 172
PstNI CAGNNNCTG 2 cut(s) 316, 1316
PsuI RGATCY 2 cut(s) 862, 1306
PvuII CAGCTG 2 cut(s) 548, 1316
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 887
SaqAI TTAA 8 cut(s) 570, 686, 728, 764, 857, 1008, 1041, 1238
SatI GCNGC 4 cut(s) 228, 989, 1121, 1314
Sau3AI GATC 4 cut(s) 862, 1219, 1243, 1306
Sau96I GGNCC 3 cut(s) 35, 93, 922
SchI GAGTC 2 cut(s) 809, 1009
ScrFI CCNGG 3 cut(s) 33, 181, 927
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 2 cut(s) 592, 772
SfcI CTRYAG 2 cut(s) 149, 168
SinI GGWCC 2 cut(s) 35, 93
SmiMI CAYNNNNRTG 1 cut(s) 887
SpeI ACTAGT 1 cut(s) 157
Sse9I AATT 8 cut(s) 210, 501, 713, 790, 808, 976, 1047, 1075
SsiI CCGC 1 cut(s) 495
SspMI CTAG 4 cut(s) 158, 254, 515, 1386
StyD4I CCNGG 3 cut(s) 31, 179, 925
StyI CCWWGG 1 cut(s) 1290
TaaI ACNGT 5 cut(s) 64, 123, 177, 691, 1141
TaiI ACGT 6 cut(s) 250, 291, 363, 486, 1069, 1096
TaqI TCGA 3 cut(s) 624, 909, 1375
TasI AATT 8 cut(s) 210, 501, 713, 790, 808, 976, 1047, 1075
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 2 cut(s) 115, 1225
Tru1I TTAA 8 cut(s) 570, 686, 728, 764, 857, 1008, 1041, 1238
Tru9I TTAA 8 cut(s) 570, 686, 728, 764, 857, 1008, 1041, 1238
TscAI CASTG 1 cut(s) 899
TseI GCWGC 4 cut(s) 227, 988, 1120, 1313
TspDTI ATGAA 4 cut(s) 597, 708, 786, 977
TspRI CASTG 1 cut(s) 899
Van91I CCANNNNNTGG 1 cut(s) 1316
VpaK11BI GGWCC 2 cut(s) 35, 93
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 4 cut(s) 501, 713, 808, 976
XbaI TCTAGA 1 cut(s) 253
XceI RCATGY 2 cut(s) 447, 995
XmiI GTMKAC 1 cut(s) 338
XmnI GAANNNNTTC 1 cut(s) 1035
XspI CTAG 4 cut(s) 158, 254, 515, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.