Rorug01G0063700

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006099
Physical Location & Seq
Reverse (-)
10446348 .. 10453162
6815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0063700.1

Sequence Viewer

Length: 2220 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTTGCAGTATTCTACTGCTTGTCGATGTTACTCACTCAACTGCTCAGGATACCCGAAAGCCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGCAGCAACATTGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGACCGGAATGGATGGTGTAGTGTCTGTCTTCCTAAGCAAACAAAGGAGCGTGAAAACAACAAAGTCATGGAACTTCCTTGGGTTTCCAGAAACAGTTAAGAGAAGCGCCCTTGAAAGGGATATCATCGTCGGTGTGATAGACAGTGGAATTTGGCCAGAGTCAGACAGCTTTAGTGATGCTGGTTTTGGTCCTCCACCTAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTGCTTGTAACAATAAAATCATTGGAGCACAGTATTACCGTGCTTCTCGATCCTTCGAAGCAGATGTTGAGTCTCCAAGAGACTCAAATGGTCATGGAACTCATACTGCATCAACGGCAGCTGGGAACACAGTGAGCAAGGCAAGCCTAAATGGTTTAGGGTTGGGAAAAGCAAGAGGAGGGGTGCCATCAACACGTATTGCCGTGTACAAAGTATGCTGGAGCAACACGCCTGCTTGTGAAGATGCTGACGTTTTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTCTCTATTTCCGTCGCGGGCAGTATTCCAGTTGATAATTTAAATGATGCATTTGCAATTGGGGCATTTCATGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAAAGTTCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGAGGTAAATTCTATCCTTTAATTTATGGTGGAGATGCACCCAATAAAAAAGCAGGTGCTAACGGGACCCTCTCCGGGTTTTGTCTTCAAGATACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCTATCGATGGGGCTGGGGGTGGGGCCATATTGGGTGGTGCAGCAGGAATTGTTCTGATGGGACAAAAAGTCGCCGATGAAGTGCTTGGCCCTCTTCCGGTGCCTGCATCACACGTCGGGTTGGAAGAACATAGCGACATTTACAAATACATAAAATTAACAAGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCCTACTCATCAAGGGGTCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTAGCGGCATATCCCCCAAGTGGTGGTGGCAGTAGAGCTGGATACTATTTAGAAACCGGGACATCAATGGCGTGCCCCCATGCTACAGCCGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCATCGGCTGCCATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTTCCAAAACTAGCCCTGATGCTGAATTTGCATACGGAGCTGGCCTGATAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTGTACAATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGAAAGCCATAACCAAGGACAAGACTAGTTGTTCATCAAAATCTAATAATGGAACAGCCAATGACCTGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCAAAATTTGTTAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCGAATTCCACATACAGAGTTAAAGTGGTGGCTCCATTAGGACTCAAAATCAATGTGAATCCAAATGTGCTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATTTAGTCTCTGCGTCTTTGATGTGGGATGATGGTGTTTTCCAAGTCAAGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0023052 GO:0030422 GO:0031047 GO:0031050 GO:0031503 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035556 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071369 GO:0071407 GO:0071426 GO:0071427 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0098542 GO:1901360 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
ko03013 ko03040 map03013 map03040
Pfam Domains
Protein Families
efThoc1

Protein Analysis

739

Amino Acids

78.63

Weight (kDa)

8.3

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 112 1.3e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 582 5.2e-42 Subtilase family
fn3_6 PF17766 638 - 735 3.9e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1124
Acc36I ACCTGC 1 cut(s) 1124
Acc65I GGTACC 1 cut(s) 133
AccB1I GGYRCC 3 cut(s) 133, 706, 1321
AccB7I CCANNNNNTGG 2 cut(s) 1550, 1775
AccI GTMKAC 1 cut(s) 813
AccII CGCG 1 cut(s) 836
AciI CCGC 7 cut(s) 782, 836, 924, 1006, 1508, 1532, 1689
AclWI GGATC 4 cut(s) 567, 1943, 1956, 2005
AcoI YGGCCR 2 cut(s) 440, 921
AcsI RAATTY 6 cut(s) 435, 1088, 1727, 1955, 2005, 2140
AfaI GTAC 3 cut(s) 135, 731, 1787
AflIII ACRYGT 2 cut(s) 716, 1333
AgsI TTSAA 5 cut(s) 244, 401, 1028, 1169, 1852
AhdI GACNNNNNGTC 1 cut(s) 1289
AhlI ACTAGT 1 cut(s) 1874
AjiI CACGTC 1 cut(s) 1336
AjnI CCWGG 2 cut(s) 1513, 1774
AluBI AGCT 7 cut(s) 212, 228, 456, 644, 1565, 1622, 1742
AluI AGCT 7 cut(s) 212, 228, 456, 644, 1565, 1622, 1742
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 3 cut(s) 597, 600, 2152
AlwI GGATC 4 cut(s) 567, 1943, 1956, 2005
Ama87I CYCGRG 1 cut(s) 2087
AoxI GGCC 7 cut(s) 440, 921, 1219, 1245, 1309, 1744, 2123
ApeKI GCWGC 7 cut(s) 186, 254, 641, 989, 1262, 1622, 1661
ApoI RAATTY 6 cut(s) 435, 1088, 1727, 1955, 2005, 2140
AseI ATTAAT 1 cut(s) 1422
Asp718I GGTACC 1 cut(s) 133
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 8 cut(s) 476, 938, 1146, 1220, 1245, 1310, 1466, 2123
AsuC2I CCSGG 3 cut(s) 942, 1156, 1585
AsuHPI GGTGA 2 cut(s) 1009, 1634
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 2087
AvaII GGWCC 4 cut(s) 476, 938, 1146, 1466
BaeGI GKGCMC 2 cut(s) 1449, 1604
BaeI ACNNNNGTAYC 2 cut(s) 1825, 1858
BalI TGGCCA 1 cut(s) 442
BamHI GGATCC 1 cut(s) 1948
BanI GGYRCC 3 cut(s) 133, 706, 1321
BanII GRGCYC 2 cut(s) 1768, 2195
BbsI GAAGAC 3 cut(s) 307, 1157, 1963
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 7 cut(s) 198, 241, 653, 976, 1274, 1609, 1648
BceAI ACGGC 3 cut(s) 654, 710, 1601
BciT130I CCWGG 2 cut(s) 1515, 1776
BciVI GTATCC 3 cut(s) 76, 1562, 1826
BclI TGATCA 1 cut(s) 1191
BcnI CCSGG 3 cut(s) 942, 1156, 1585
BcoDI GTCTC 3 cut(s) 597, 600, 2152
BcuI ACTAGT 1 cut(s) 1874
BfaI CTAG 4 cut(s) 1529, 1713, 1761, 1875
BfmI CTRYAG 2 cut(s) 167, 1611
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 1124
BfuI GTATCC 3 cut(s) 76, 1562, 1826
Bme1390I CCNGG 5 cut(s) 942, 1156, 1515, 1585, 1776
Bme18I GGWCC 4 cut(s) 476, 938, 1146, 1466
BmeRI GACNNNNNGTC 1 cut(s) 1289
BmeT110I CYCGRG 1 cut(s) 2087
BmgBI CACGTC 1 cut(s) 1336
BmgT120I GGNCC 8 cut(s) 476, 938, 1146, 1220, 1245, 1310, 1466, 2123
BmrFI CCNGG 5 cut(s) 942, 1156, 1515, 1585, 1776
BmsI GCATC 8 cut(s) 454, 641, 757, 784, 856, 1105, 1337, 1711
BpiI GAAGAC 3 cut(s) 307, 1157, 1963
BpmI CTGGAG 2 cut(s) 763, 1497
Bpu10I CCTNAGC 2 cut(s) 78, 320
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 1478
BpuMI CCSGG 3 cut(s) 942, 1156, 1585
Bsa29I ATCGAT 1 cut(s) 1227
BsaAI YACGTR 1 cut(s) 719
BsaJI CCNNGG 5 cut(s) 364, 918, 971, 1081, 1863
BsaWI WCCGGW 2 cut(s) 290, 1318
Bse1I ACTGG 1 cut(s) 848
Bse3DI GCAATG 3 cut(s) 264, 520, 798
BseBI CCWGG 2 cut(s) 1515, 1776
BseCI ATCGAT 1 cut(s) 1227
BseDI CCNNGG 5 cut(s) 364, 918, 971, 1081, 1863
BseGI GGATG 3 cut(s) 304, 1665, 2173
BseMI GCAATG 3 cut(s) 264, 520, 798
BseMII CTCAG 2 cut(s) 40, 92
BseNI ACTGG 1 cut(s) 848
BseRI GAGGAG 1 cut(s) 714
BseSI GKGCMC 2 cut(s) 1449, 1604
BseXI GCAGC 7 cut(s) 198, 241, 653, 976, 1274, 1609, 1648
BseYI CCCAGC 2 cut(s) 644, 1235
BsgI GTGCAG 1 cut(s) 1281
Bsh1236I CGCG 1 cut(s) 836
BshFI GGCC 7 cut(s) 442, 923, 1221, 1247, 1311, 1746, 2125
BshNI GGYRCC 3 cut(s) 133, 706, 1321
BshVI ATCGAT 1 cut(s) 1227
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 2087
BsiSI CCGG 5 cut(s) 291, 941, 1155, 1319, 1584
BslFI GGGAC 4 cut(s) 1068, 1159, 1296, 1600
BsmAI GTCTC 3 cut(s) 597, 600, 2152
BsmFI GGGAC 4 cut(s) 1068, 1159, 1296, 1600
BsmI GAATGC 1 cut(s) 785
BsnI GGCC 7 cut(s) 442, 923, 1221, 1247, 1311, 1746, 2125
BsoBI CYCGRG 1 cut(s) 2087
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 5 cut(s) 553, 1449, 1604, 1768, 2195
Bsp1407I TGTACA 2 cut(s) 729, 1785
Bsp143I GATC 6 cut(s) 148, 572, 1191, 1476, 1948, 1997
Bsp19I CCATGG 2 cut(s) 918, 971
BspACI CCGC 7 cut(s) 782, 836, 924, 1006, 1508, 1532, 1689
BspANI GGCC 7 cut(s) 442, 923, 1221, 1247, 1311, 1746, 2125
BspCNI CTCAG 2 cut(s) 39, 91
BspDI ATCGAT 1 cut(s) 1227
BspFNI CGCG 1 cut(s) 836
BspMI ACCTGC 1 cut(s) 1124
BspPI GGATC 4 cut(s) 567, 1943, 1956, 2005
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 3 cut(s) 133, 706, 1321
BsrDI GCAATG 3 cut(s) 264, 520, 798
BsrGI TGTACA 2 cut(s) 729, 1785
BsrI ACTGG 1 cut(s) 848
BssECI CCNNGG 5 cut(s) 364, 918, 971, 1081, 1863
BssMI GATC 6 cut(s) 148, 572, 1191, 1476, 1948, 1997
BssT1I CCWWGG 4 cut(s) 364, 918, 971, 1863
Bst2UI CCWGG 2 cut(s) 1515, 1776
Bst6I CTCTTC 3 cut(s) 12, 1320, 1410
BstAPI GCANNNNNTGC 1 cut(s) 890
BstAUI TGTACA 2 cut(s) 729, 1785
BstBAI YACGTR 1 cut(s) 719
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 6 cut(s) 667, 756, 838, 1326, 1600, 1744
BstDEI CTNAG 5 cut(s) 26, 78, 320, 486, 1177
BstDSI CCRYGG 2 cut(s) 918, 971
BstF5I GGATG 3 cut(s) 304, 1665, 2173
BstFNI CGCG 1 cut(s) 836
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 6 cut(s) 151, 575, 1194, 1479, 1951, 2000
BstMAI GTCTC 3 cut(s) 597, 600, 2152
BstMBI GATC 6 cut(s) 148, 572, 1191, 1476, 1948, 1997
BstNI CCWGG 2 cut(s) 1515, 1776
BstSCI CCNGG 5 cut(s) 940, 1154, 1513, 1583, 1774
BstSFI CTRYAG 2 cut(s) 167, 1611
BstSLI GKGCMC 2 cut(s) 1449, 1604
BstUI CGCG 1 cut(s) 836
BstV1I GCAGC 7 cut(s) 198, 241, 653, 976, 1274, 1609, 1648
BstV2I GAAGAC 3 cut(s) 307, 1157, 1963
BstX2I RGATCY 1 cut(s) 1948
BstYI RGATCY 1 cut(s) 1948
Bsu15I ATCGAT 1 cut(s) 1227
BsuI GTATCC 3 cut(s) 76, 1562, 1826
BsuRI GGCC 7 cut(s) 442, 923, 1221, 1247, 1311, 1746, 2125
BsuTUI ATCGAT 1 cut(s) 1227
BtgI CCRYGG 2 cut(s) 918, 971
BtrI CACGTC 1 cut(s) 1336
BtsCI GGATG 3 cut(s) 304, 1665, 2173
BtsIMutI CAGTG 3 cut(s) 436, 660, 1843
BveI ACCTGC 1 cut(s) 1124
Cac8I GCNNGC 6 cut(s) 667, 756, 838, 1326, 1600, 1744
CfoI GCGC 1 cut(s) 395
Cfr13I GGNCC 8 cut(s) 476, 938, 1146, 1220, 1245, 1310, 1466, 2123
ClaI ATCGAT 1 cut(s) 1227
CseI GACGC 1 cut(s) 2142
Csp6I GTAC 3 cut(s) 134, 730, 1786
CviAII CATG 8 cut(s) 155, 206, 354, 617, 890, 919, 972, 1607
CviQI GTAC 3 cut(s) 134, 730, 1786
DdeI CTNAG 5 cut(s) 26, 78, 320, 486, 1177
DpnI GATC 6 cut(s) 150, 574, 1193, 1478, 1950, 1999
DpnII GATC 6 cut(s) 148, 572, 1191, 1476, 1948, 1997
DraI TTTAAA 1 cut(s) 861
DriI GACNNNNNGTC 1 cut(s) 1289
EaeI YGGCCR 2 cut(s) 440, 921
Eam1104I CTCTTC 3 cut(s) 12, 1320, 1410
Eam1105I GACNNNNNGTC 1 cut(s) 1289
EarI CTCTTC 3 cut(s) 12, 1320, 1410
Eco130I CCWWGG 4 cut(s) 364, 918, 971, 1863
Eco24I GRGCYC 2 cut(s) 1768, 2195
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 4 cut(s) 476, 938, 1146, 1466
Eco88I CYCGRG 1 cut(s) 2087
EcoO109I RGGNCCY 1 cut(s) 1146
EcoRI GAATTC 1 cut(s) 2005
EcoRII CCWGG 2 cut(s) 1513, 1774
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 4 cut(s) 364, 918, 971, 1863
EcoT22I ATGCAT 1 cut(s) 871
EcoT38I GRGCYC 2 cut(s) 1768, 2195
ErhI CCWWGG 4 cut(s) 364, 918, 971, 1863
FaeI CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1610
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 4 cut(s) 1068, 1159, 1296, 1600
FatI CATG 8 cut(s) 154, 205, 353, 616, 889, 918, 971, 1606
FauI CCCGC 1 cut(s) 829
FauNDI CATATG 1 cut(s) 1441
FbaI TGATCA 1 cut(s) 1191
FblI GTMKAC 1 cut(s) 813
FokI GGATG 3 cut(s) 311, 1652, 2180
FriOI GRGCYC 2 cut(s) 1768, 2195
FspBI CTAG 4 cut(s) 1529, 1713, 1761, 1875
GlaI GCGC 1 cut(s) 394
GsaI CCCAGC 2 cut(s) 648, 1239
GsuI CTGGAG 2 cut(s) 763, 1497
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 7 cut(s) 442, 923, 1221, 1247, 1311, 1746, 2125
HapII CCGG 5 cut(s) 291, 941, 1155, 1319, 1584
HgaI GACGC 1 cut(s) 2142
HhaI GCGC 1 cut(s) 395
Hin1II CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1610
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 2 cut(s) 181, 814
HindII GTYRAC 2 cut(s) 181, 814
HinfI GANTC 5 cut(s) 446, 593, 605, 2043, 2059
HpaII CCGG 5 cut(s) 291, 941, 1155, 1319, 1584
HphI GGTGA 2 cut(s) 1009, 1634
Hpy166II GTNNAC 7 cut(s) 181, 730, 814, 1786, 1826, 1844, 2079
Hpy188I TCNGA 4 cut(s) 451, 1082, 1278, 1476
Hpy188III TCNNGA 6 cut(s) 80, 374, 570, 1169, 1793, 2188
Hpy8I GTNNAC 7 cut(s) 181, 730, 814, 1786, 1826, 1844, 2079
Hpy99I CGWCG 4 cut(s) 419, 836, 1340, 2005
HpyAV CCTTC 5 cut(s) 32, 586, 929, 1767, 1935
HpyCH4IV ACGT 5 cut(s) 174, 718, 774, 1335, 1629
HpyF3I CTNAG 5 cut(s) 26, 78, 320, 486, 1177
HpySE526I ACGT 5 cut(s) 174, 718, 774, 1335, 1629
Hsp92II CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1610
HspAI GCGC 1 cut(s) 393
KflI GGGWCCC 1 cut(s) 1146
KpnI GGTACC 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 1191
Kzo9I GATC 6 cut(s) 148, 572, 1191, 1476, 1948, 1997
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 9 cut(s) 333, 548, 744, 944, 1441, 1516, 1739, 1771, 2038
Lsp1109I GCAGC 7 cut(s) 198, 241, 653, 976, 1274, 1609, 1648
LweI GCATC 8 cut(s) 454, 641, 757, 784, 856, 1105, 1337, 1711
MaeI CTAG 4 cut(s) 1529, 1713, 1761, 1875
MaeII ACGT 5 cut(s) 174, 718, 774, 1335, 1629
MaeIII GTNAC 7 cut(s) 61, 530, 929, 955, 1034, 1984, 2110
MalI GATC 6 cut(s) 150, 574, 1193, 1478, 1950, 1999
MboI GATC 6 cut(s) 148, 572, 1191, 1476, 1948, 1997
MfeI CAATTG 2 cut(s) 876, 1028
MflI RGATCY 1 cut(s) 1948
MhlI GDGCHC 5 cut(s) 553, 1449, 1604, 1768, 2195
MlsI TGGCCA 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 4 cut(s) 455, 599, 602, 2037
MmeI TCCRAC 2 cut(s) 1323, 1975
Mox20I TGGCCA 1 cut(s) 442
Mph1103I ATGCAT 1 cut(s) 871
MscI TGGCCA 1 cut(s) 442
MseI TTAA 8 cut(s) 384, 860, 1100, 1379, 1422, 1962, 2022, 2218
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 2 cut(s) 644, 926
MspI CCGG 5 cut(s) 291, 941, 1155, 1319, 1584
MspR9I CCNGG 5 cut(s) 942, 1156, 1515, 1585, 1776
MunI CAATTG 2 cut(s) 876, 1028
Mva1269I GAATGC 1 cut(s) 785
MvaI CCWGG 2 cut(s) 1515, 1776
MvnI CGCG 1 cut(s) 836
NciI CCSGG 3 cut(s) 942, 1156, 1585
NcoI CCATGG 2 cut(s) 918, 971
NdeI CATATG 1 cut(s) 1441
NdeII GATC 6 cut(s) 148, 572, 1191, 1476, 1948, 1997
NlaIII CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1610
NmuCI GTSAC 3 cut(s) 955, 1984, 2110
NsiI ATGCAT 1 cut(s) 871
NspV TTCGAA 1 cut(s) 579
PaqCI CACCTGC 1 cut(s) 1124
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 785
PfeI GAWTC 1 cut(s) 2059
PflFI GACNNNGTC 1 cut(s) 809
PflMI CCANNNNNTGG 2 cut(s) 1550, 1775
PfoI TCCNGGA 2 cut(s) 940, 1513
PleI GAGTC 4 cut(s) 454, 599, 601, 2037
PpsI GAGTC 4 cut(s) 454, 599, 601, 2037
Ppu21I YACGTR 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 1146
PshBI ATTAAT 1 cut(s) 1422
Psp5II RGGWCCY 1 cut(s) 1146
Psp6I CCWGG 2 cut(s) 1513, 1774
PspFI CCCAGC 2 cut(s) 644, 1235
PspGI CCWGG 2 cut(s) 1513, 1774
PspPI GGNCC 8 cut(s) 476, 938, 1146, 1220, 1245, 1310, 1466, 2123
PspPPI RGGWCCY 1 cut(s) 1146
PsuI RGATCY 1 cut(s) 1948
PsyI GACNNNGTC 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 3 cut(s) 135, 731, 1787
RsaNI GTAC 3 cut(s) 134, 730, 1786
SalI GTCGAC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 8 cut(s) 384, 860, 1100, 1379, 1422, 1962, 2022, 2218
Sau3AI GATC 6 cut(s) 148, 572, 1191, 1476, 1948, 1997
Sau96I GGNCC 8 cut(s) 476, 938, 1146, 1220, 1245, 1310, 1466, 2123
SchI GAGTC 4 cut(s) 455, 599, 602, 2037
ScrFI CCNGG 5 cut(s) 942, 1156, 1515, 1585, 1776
SduI GDGCHC 5 cut(s) 553, 1449, 1604, 1768, 2195
SfaNI GCATC 8 cut(s) 454, 641, 757, 784, 856, 1105, 1337, 1711
SfcI CTRYAG 2 cut(s) 167, 1611
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 4 cut(s) 476, 938, 1146, 1466
SmiI ATTTAAAT 1 cut(s) 861
SmlI CTYRAG 1 cut(s) 1493
SmoI CTYRAG 1 cut(s) 1493
SpeI ACTAGT 1 cut(s) 1874
SsiI CCGC 7 cut(s) 782, 836, 924, 1006, 1508, 1532, 1689
SspMI CTAG 4 cut(s) 1529, 1713, 1761, 1875
StyD4I CCNGG 5 cut(s) 940, 1154, 1513, 1583, 1774
StyI CCWWGG 4 cut(s) 364, 918, 971, 1863
SwaI ATTTAAAT 1 cut(s) 861
TaiI ACGT 5 cut(s) 177, 721, 777, 1338, 1632
TaqI TCGA 8 cut(s) 10, 57, 571, 579, 789, 813, 1227, 2003
TatI WGTACW 2 cut(s) 729, 1785
TauI GCSGC 4 cut(s) 785, 926, 1511, 1535
TfiI GAWTC 1 cut(s) 2059
Tru1I TTAA 8 cut(s) 384, 860, 1100, 1379, 1422, 1962, 2022, 2218
Tru9I TTAA 8 cut(s) 384, 860, 1100, 1379, 1422, 1962, 2022, 2218
TscAI CASTG 3 cut(s) 436, 660, 1843
TseFI GTSAC 3 cut(s) 955, 1984, 2110
TseI GCWGC 7 cut(s) 186, 254, 641, 989, 1262, 1622, 1661
Tsp45I GTSAC 3 cut(s) 955, 1984, 2110
TspDTI ATGAA 7 cut(s) 143, 222, 878, 1003, 1314, 1812, 1872
TspGWI ACGGA 2 cut(s) 820, 1752
TspRI CASTG 3 cut(s) 436, 660, 1843
Tth111I GACNNNGTC 1 cut(s) 809
Van91I CCANNNNNTGG 2 cut(s) 1550, 1775
VpaK11BI GGWCC 4 cut(s) 476, 938, 1146, 1466
VspI ATTAAT 1 cut(s) 1422
XapI RAATTY 6 cut(s) 435, 1088, 1727, 1955, 2005, 2140
XcmI CCANNNNNNNNNTGG 2 cut(s) 489, 1027
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 4 cut(s) 1529, 1713, 1761, 1875
Zsp2I ATGCAT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.