MD14G1005100.v1.1

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015701
Physical Location & Seq
Forward (+)
547737 .. 548449
713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1005100.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGATTCCAACGGTGAAGTGGTTAACATTATACGTTCTTACGAGGATTTTATGCCTTATTGCAAGTTCCTAAAAGAGGAACGATTCGACATTTACCAGATTCCTCTTCATGGTTTCAAGATGGAACAATTCAAGCTATTTGTGAACGACAACAAAGGAACAATAAAGATTGAAGGCCAACGTCCTAAGGGTGGAAGCAGATGGAGCCGCTTCCGCCAAAAATTCAAACTTCCTTGCAATGTTTATAACACTAAGGATATTCATGCAAGTTTTGGCAATGGTGTCCTTACCATAATACTGCCAAAAAGGGAACCATTGATGATTTCGAATTCCGACGATAAGTCTGATCTATTTGTCTACAACGGTAAGCTTTTCAGGCTGAAAATCAGCAAAAGAGGAATCCTCGGATCAATTGCACTTGTTGCTGTGGGCGTGGCCGTTGGAGGTTATGTAATCCTAAAACACTTGCCATCTACGCATGCTGCTGAGAAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

166

Amino Acids

18.82

Weight (kDa)

9.67

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 106 5.5e-08 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015701)

Species Orthologous Gene IDs
malus_domestica MD14G1005100.v1.1 MD14G1005200.v1.1
prunus_persica Prupe.7G008400_v2.0.a1
pyrus_communis pycom14g00340 pycom14g00350 pycom14g00360 pycom14g00370
rosa_chinensis RchiOBHm_Chr3g0496541
rosa_laevigata RLG00000022520
rosa_multiflora Rmu_sc0000741.1_g000008
rosa_roxburghii Rroxscaffold_6G00388950
rosa_rugosa Rorug03G0283900
rosa_samantha Rh3BG365300 Rh3CG362600 Rh3DG365700
rosa_wichuraiana Rw3G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 246
AccI GTMKAC 1 cut(s) 357
AciI CCGC 2 cut(s) 208, 214
AclWI GGATC 1 cut(s) 415
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 2 cut(s) 221, 328
AfiI CCNNNNNNNGG 3 cut(s) 76, 110, 191
AgsI TTSAA 4 cut(s) 118, 133, 173, 226
AhdI GACNNNNNGTC 1 cut(s) 340
AluBI AGCT 2 cut(s) 136, 370
AluI AGCT 2 cut(s) 136, 370
AlwI GGATC 1 cut(s) 415
AoxI GGCC 2 cut(s) 175, 435
ApeKI GCWGC 1 cut(s) 482
ApoI RAATTY 2 cut(s) 221, 328
AsuHPI GGTGA 1 cut(s) 26
AsuII TTCGAA 1 cut(s) 326
AxyI CCTNAGG 1 cut(s) 186
BbvI GCAGC 1 cut(s) 469
BccI CCATC 3 cut(s) 115, 195, 478
BceAI ACGGC 1 cut(s) 422
BisI GCNGC 2 cut(s) 208, 483
BlsI GCNGC 2 cut(s) 209, 484
BmeRI GACNNNNNGTC 1 cut(s) 340
BmiI GGNNCC 2 cut(s) 206, 312
BplI GAGNNNNNCTC 2 cut(s) 387, 419
Bpu14I TTCGAA 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 110, 191
Bse21I CCTNAGG 1 cut(s) 186
Bse3DI GCAATG 2 cut(s) 244, 283
BseDI CCNNGG 1 cut(s) 403
BseLI CCNNNNNNNGG 3 cut(s) 76, 110, 191
BseMI GCAATG 2 cut(s) 244, 283
BseMII CTCAG 1 cut(s) 477
BseXI GCAGC 1 cut(s) 469
BshFI GGCC 2 cut(s) 177, 437
BslI CCNNNNNNNGG 3 cut(s) 76, 110, 191
BsnI GGCC 2 cut(s) 177, 437
Bsp119I TTCGAA 1 cut(s) 326
Bsp143I GATC 2 cut(s) 346, 407
BspACI CCGC 2 cut(s) 208, 214
BspANI GGCC 2 cut(s) 177, 437
BspCNI CTCAG 1 cut(s) 478
BspLI GGNNCC 2 cut(s) 206, 312
BspPI GGATC 1 cut(s) 415
BspT104I TTCGAA 1 cut(s) 326
BsrDI GCAATG 2 cut(s) 244, 283
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 2 cut(s) 346, 407
Bst4CI ACNGT 2 cut(s) 14, 365
Bst6I CTCTTC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 422
BstBI TTCGAA 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 3 cut(s) 186, 252, 486
BstENI CCTNNNNNAGG 1 cut(s) 74
BstKTI GATC 2 cut(s) 349, 410
BstMBI GATC 2 cut(s) 346, 407
BstMWI GCNNNNNNNGC 5 cut(s) 204, 213, 376, 422, 475
BstNSI RCATGY 1 cut(s) 482
BstV1I GCAGC 1 cut(s) 469
Bsu36I CCTNAGG 1 cut(s) 186
BsuRI GGCC 2 cut(s) 177, 437
Cac8I GCNNGC 1 cut(s) 480
CviAII CATG 3 cut(s) 110, 263, 479
CviJI RGCY 6 cut(s) 136, 177, 207, 370, 379, 437
CviKI_1 RGCY 6 cut(s) 136, 177, 207, 370, 379, 437
DdeI CTNAG 3 cut(s) 186, 252, 486
DpnI GATC 2 cut(s) 348, 409
DpnII GATC 2 cut(s) 346, 407
DriI GACNNNNNGTC 1 cut(s) 340
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 111
Eam1105I GACNNNNNGTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 203
Eco81I CCTNAGG 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoRI GAATTC 1 cut(s) 328
FaeI CATG 3 cut(s) 113, 266, 482
FaiI YATR 8 cut(s) 32, 53, 111, 246, 264, 293, 450, 480
FatI CATG 3 cut(s) 109, 262, 478
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 208, 483
Fsp4HI GCNGC 2 cut(s) 208, 483
GluI GCNGC 2 cut(s) 208, 483
HaeIII GGCC 2 cut(s) 177, 437
Hin1II CATG 3 cut(s) 113, 266, 482
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 4 cut(s) 5, 84, 100, 399
HpaI GTTAAC 1 cut(s) 25
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 3 cut(s) 25, 145, 358
Hpy188I TCNGA 3 cut(s) 334, 346, 407
Hpy188III TCNNGA 1 cut(s) 118
Hpy8I GTNNAC 3 cut(s) 25, 145, 358
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 2 cut(s) 14, 365
HpyCH4IV ACGT 2 cut(s) 34, 181
HpyCH4V TGCA 4 cut(s) 63, 237, 266, 416
HpyF10VI GCNNNNNNNGC 5 cut(s) 204, 213, 376, 422, 475
HpyF3I CTNAG 3 cut(s) 186, 252, 486
HpySE526I ACGT 2 cut(s) 34, 181
Hsp92II CATG 3 cut(s) 113, 266, 482
KspAI GTTAAC 1 cut(s) 25
Kzo9I GATC 2 cut(s) 346, 407
LmnI GCTCC 1 cut(s) 204
LpnPI CCDG 2 cut(s) 110, 361
Lsp1109I GCAGC 1 cut(s) 469
MaeII ACGT 2 cut(s) 34, 181
MalI GATC 2 cut(s) 348, 409
MboI GATC 2 cut(s) 346, 407
MboII GAAGA 1 cut(s) 98
MfeI CAATTG 1 cut(s) 411
MluCI AATT 4 cut(s) 128, 221, 328, 411
MmeI TCCRAC 3 cut(s) 33, 357, 421
MnlI CCTC 6 cut(s) 37, 70, 114, 389, 413, 437
MseI TTAA 2 cut(s) 24, 496
MunI CAATTG 1 cut(s) 411
MwoI GCNNNNNNNGC 5 cut(s) 204, 213, 376, 422, 475
NdeII GATC 2 cut(s) 346, 407
NlaIII CATG 3 cut(s) 113, 266, 482
NlaIV GGNNCC 2 cut(s) 206, 312
NspI RCATGY 1 cut(s) 482
NspV TTCGAA 1 cut(s) 326
PaeI GCATGC 1 cut(s) 482
PfeI GAWTC 4 cut(s) 5, 84, 100, 399
PkrI GCNGC 2 cut(s) 209, 484
PsiI TTATAA 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 206, 312
SaqAI TTAA 2 cut(s) 24, 496
SatI GCNGC 2 cut(s) 208, 483
Sau3AI GATC 2 cut(s) 346, 407
SetI ASST 5 cut(s) 37, 138, 184, 372, 448
SfuI TTCGAA 1 cut(s) 326
SphI GCATGC 1 cut(s) 482
Sse9I AATT 4 cut(s) 128, 221, 328, 411
SsiI CCGC 2 cut(s) 208, 214
TaaI ACNGT 2 cut(s) 14, 365
TaiI ACGT 2 cut(s) 37, 184
TaqI TCGA 2 cut(s) 87, 326
TasI AATT 4 cut(s) 128, 221, 328, 411
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 4 cut(s) 5, 84, 100, 399
Tru1I TTAA 2 cut(s) 24, 496
Tru9I TTAA 2 cut(s) 24, 496
TseI GCWGC 1 cut(s) 482
TspDTI ATGAA 2 cut(s) 98, 251
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 2 cut(s) 221, 328
XceI RCATGY 1 cut(s) 482
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XmiI GTMKAC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.