RchiOBHm_Chr3g0496541

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Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0015701
Physical Location & Seq
Reverse (-)
47524484 .. 47525059
576 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45887

Sequence Viewer

Length: 456 bp
ATGGATTCCAAAAGTGAAGTGGTTACCATCATACGATCTTATGAAGATTTCATGCCTTATTGCAAGCTCCAAAAAGAAGAACTACGTGACACTTACCAGATTCCTCTCCATGGTTTCAAGATCGAACAATTCAAGGTCTGTGTTAACGACAAGAAAGGAACGATAAAGATTGAAGGCGAACGTCCTGTGGATGGAAATAGATGGAGCCGCTTCCGCCAAAGTTTCAAAGCTTCTCCTGATGTTTATAGAGTCAAGGATATTTCTGCAAGGTTTCAGAATGGTGTTCTTTCTCTAATATTACCAAAAACCAAACCTTCAAATGATCACACATCTAATCGATTTCCGTTTGCGAACACGAGTTTGCTTCTGAGGATTGCACCAGCTCTTGTTGTGATGGTCGCTGTTGGAGGTTATGGAATCTTCAAATATTTCACATCGCATGTTATAGGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

151

Amino Acids

17.27

Weight (kDa)

9.6

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 34 - 109 9e-06 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015701)

Species Orthologous Gene IDs
malus_domestica MD14G1005100.v1.1 MD14G1005200.v1.1
prunus_persica Prupe.7G008400_v2.0.a1
pyrus_communis pycom14g00340 pycom14g00350 pycom14g00360 pycom14g00370
rosa_chinensis RchiOBHm_Chr3g0496541
rosa_laevigata RLG00000022520
rosa_multiflora Rmu_sc0000741.1_g000008
rosa_roxburghii Rroxscaffold_6G00388950
rosa_rugosa Rorug03G0283900
rosa_samantha Rh3BG365300 Rh3CG362600 Rh3DG365700
rosa_wichuraiana Rw3G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 208, 214
AfiI CCNNNNNNNGG 2 cut(s) 110, 191
AgsI TTSAA 6 cut(s) 118, 133, 173, 226, 318, 424
AluBI AGCT 4 cut(s) 67, 230, 383, 453
AluI AGCT 4 cut(s) 67, 230, 383, 453
BauI CACGAG 1 cut(s) 355
BccI CCATC 4 cut(s) 35, 185, 195, 388
BclI TGATCA 1 cut(s) 322
BisI GCNGC 1 cut(s) 208
BlsI GCNGC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 206
Bsa29I ATCGAT 1 cut(s) 337
BsaAI YACGTR 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 109
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 191
BseCI ATCGAT 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 109
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 2 cut(s) 110, 191
BseMII CTCAG 1 cut(s) 359
BshVI ATCGAT 1 cut(s) 337
BslI CCNNNNNNNGG 2 cut(s) 110, 191
Bsp143I GATC 3 cut(s) 35, 120, 322
Bsp19I CCATGG 1 cut(s) 109
BspACI CCGC 2 cut(s) 208, 214
BspCNI CTCAG 1 cut(s) 360
BspDI ATCGAT 1 cut(s) 337
BspLI GGNNCC 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 3 cut(s) 35, 120, 322
BssSI CACGAG 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 355
BstBAI YACGTR 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 1 cut(s) 368
BstDSI CCRYGG 1 cut(s) 109
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 1 cut(s) 196
BstKTI GATC 3 cut(s) 38, 123, 325
BstMBI GATC 3 cut(s) 35, 120, 322
BstMWI GCNNNNNNNGC 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 443
BstPI GGTNACC 1 cut(s) 22
Bsu15I ATCGAT 1 cut(s) 337
BsuTUI ATCGAT 1 cut(s) 337
BtgI CCRYGG 1 cut(s) 109
BtgZI GCGATG 1 cut(s) 420
BtsCI GGATG 1 cut(s) 196
Cac8I GCNNGC 1 cut(s) 65
ClaI ATCGAT 1 cut(s) 337
CviAII CATG 3 cut(s) 52, 110, 440
CviJI RGCY 5 cut(s) 67, 207, 230, 383, 453
CviKI_1 RGCY 5 cut(s) 67, 207, 230, 383, 453
DdeI CTNAG 1 cut(s) 368
DpnI GATC 3 cut(s) 37, 122, 324
DpnII GATC 3 cut(s) 35, 120, 322
EciI GGCGGA 1 cut(s) 203
Eco130I CCWWGG 1 cut(s) 109
Eco91I GGTNACC 1 cut(s) 22
EcoO65I GGTNACC 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 109
FaeI CATG 3 cut(s) 55, 113, 443
FaiI YATR 8 cut(s) 32, 42, 53, 111, 246, 414, 441, 446
FatI CATG 3 cut(s) 51, 109, 439
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 208
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 208
GluI GCNGC 1 cut(s) 208
Hin1II CATG 3 cut(s) 55, 113, 443
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 4 cut(s) 5, 100, 249, 417
HpaI GTTAAC 1 cut(s) 145
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 276, 369
Hpy188III TCNNGA 2 cut(s) 118, 236
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 2 cut(s) 167, 324
HpyCH4IV ACGT 2 cut(s) 85, 181
HpyCH4V TGCA 3 cut(s) 63, 266, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 213
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 2 cut(s) 85, 181
Hsp92II CATG 3 cut(s) 55, 113, 443
Ksp22I TGATCA 1 cut(s) 322
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 3 cut(s) 35, 120, 322
LmnI GCTCC 3 cut(s) 72, 204, 450
LpnPI CCDG 4 cut(s) 110, 198, 249, 393
MaeII ACGT 2 cut(s) 85, 181
MaeIII GTNAC 2 cut(s) 22, 86
MalI GATC 3 cut(s) 37, 122, 324
MboI GATC 3 cut(s) 35, 120, 322
MboII GAAGA 3 cut(s) 56, 89, 412
MluCI AATT 1 cut(s) 128
MlyI GAGTC 1 cut(s) 258
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 3 cut(s) 114, 363, 401
MseI TTAA 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 213
NcoI CCATGG 1 cut(s) 109
NdeII GATC 3 cut(s) 35, 120, 322
NlaIII CATG 3 cut(s) 55, 113, 443
NlaIV GGNNCC 1 cut(s) 206
NmuCI GTSAC 1 cut(s) 86
NspI RCATGY 1 cut(s) 443
PfeI GAWTC 3 cut(s) 5, 100, 417
PkrI GCNGC 1 cut(s) 209
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 86
PspEI GGTNACC 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 1 cut(s) 208
Sau3AI GATC 3 cut(s) 35, 120, 322
SchI GAGTC 1 cut(s) 258
Sse9I AATT 1 cut(s) 128
SsiI CCGC 2 cut(s) 208, 214
SspI AATATT 2 cut(s) 297, 428
StyI CCWWGG 1 cut(s) 109
TaiI ACGT 2 cut(s) 88, 184
TaqI TCGA 2 cut(s) 123, 337
TasI AATT 1 cut(s) 128
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 3 cut(s) 5, 100, 417
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseFI GTSAC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 2 cut(s) 40, 57
TspGWI ACGGA 1 cut(s) 333
XceI RCATGY 1 cut(s) 443
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.