MD14G1005200.v1.1

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015701
Physical Location & Seq
Forward (+)
550748 .. 552067
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1005200.v1.1.491

Sequence Viewer

Length: 861 bp
ATGCCTCCTCTTATGGCTTCTCTCCCAGCCACGACCTATTTGCATGAACTAGTTAGAGAATTCGGGCAAATTAGGACGAAGTTGCAATCCCCAAGGTGCCCTTCTGAGCCTCAACATCTTCAAGATCAGTGCAGGATCTTCAAAGAATTTATGCAGATGGACTTTTCTGCCTCCTTTAGCCTTAAGGCTCTTCAAGCTGCTGAGAGAGCTCTCACTGAATTATACCAAGCCCAGCAGATGACTAAGGTGCAATATGAGTCCTTCATTTCCTTCTTTAAAAATCTAAGGGCCTTGAGGGATCAACATCTTAAGGCTGAGAGGCAAGCCAACAAGGTGAGATGCTACAAGGAGAAGCACACTTGGACCTCCACTACTTTGCAGCAGCTGGTGGAGGAGGGCTCGACCATGAAAGATCGGATAATAGTGGTAGCTGCCGAGATCCAGAAATTGGAGGAACAACTTTGTGATCTGAAGGCTGAGGAGACTACTCTCTCGAGCAAGCTATACAAGAAGATCGAGGAAGTGAACATTATACATTCTTACGAGGATTTTATGCCTTATTGCAAGTTCCTAAAAGAGGAACGATTCGACACTTACCAGATTCCTTTCCATGGTTTCAAGATGGAACAATTCAAGCTATTCTTGAACGACAACAAAGGAACAATAAAGATTGAAGGCCAACGTCCTAAGGGTGGAAGTAGATGGAGCGGCTTCCGCCAAAAATTCAAACTTCCTTGCAATGTTTATAACACTAAGGATATTCATGCAAGTTTTGGCAATGGTGTCCTTACCATAAGACTGCCAAAAAGGGAACTATTGAATTTTGATGTATATTTGCTTGAAGAAACAATTACAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
HSP20

Protein Analysis

287

Amino Acids

33.66

Weight (kDa)

8.97

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 203 - 271 3.8e-06 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015701)

Species Orthologous Gene IDs
malus_domestica MD14G1005100.v1.1 MD14G1005200.v1.1
prunus_persica Prupe.7G008400_v2.0.a1
pyrus_communis pycom14g00340 pycom14g00350 pycom14g00360 pycom14g00370
rosa_chinensis RchiOBHm_Chr3g0496541
rosa_laevigata RLG00000022520
rosa_multiflora Rmu_sc0000741.1_g000008
rosa_roxburghii Rroxscaffold_6G00388950
rosa_rugosa Rorug03G0283900
rosa_samantha Rh3BG365300 Rh3CG362600 Rh3DG365700
rosa_wichuraiana Rw3G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 448
AccBSI CCGCTC 1 cut(s) 708
AciI CCGC 2 cut(s) 708, 715
AclWI GGATC 3 cut(s) 143, 306, 433
AcsI RAATTY 4 cut(s) 59, 146, 722, 820
AcuI CTGAAG 1 cut(s) 491
AfiI CCNNNNNNNGG 4 cut(s) 448, 577, 611, 692
AflII CTTAAG 2 cut(s) 182, 308
AhlI ACTAGT 1 cut(s) 49
AjuI GAANNNNNNNTTGG 2 cut(s) 85, 117
AluBI AGCT 6 cut(s) 197, 209, 385, 431, 502, 637
AluI AGCT 6 cut(s) 197, 209, 385, 431, 502, 637
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 3 cut(s) 143, 306, 433
AlwNI CAGNNNCTG 1 cut(s) 385
Ama87I CYCGRG 1 cut(s) 493
AoxI GGCC 2 cut(s) 288, 676
ApeKI GCWGC 4 cut(s) 197, 379, 382, 431
ApoI RAATTY 4 cut(s) 59, 146, 722, 820
AspS9I GGNCC 2 cut(s) 288, 363
AsuHPI GGTGA 1 cut(s) 346
AvaI CYCGRG 1 cut(s) 493
AvaII GGWCC 1 cut(s) 363
AxyI CCTNAGG 1 cut(s) 687
BaeGI GKGCMC 1 cut(s) 101
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 2 cut(s) 211, 401
Bbv12I GWGCWC 1 cut(s) 211
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 4 cut(s) 184, 391, 394, 418
BccI CCATC 3 cut(s) 151, 616, 696
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BcoDI GTCTC 1 cut(s) 476
BcuI ACTAGT 1 cut(s) 49
BfaI CTAG 1 cut(s) 50
BfrI CTTAAG 2 cut(s) 182, 308
BisI GCNGC 5 cut(s) 198, 380, 383, 432, 709
BlsI GCNGC 5 cut(s) 199, 381, 384, 433, 710
Bme18I GGWCC 1 cut(s) 363
BmeT110I CYCGRG 1 cut(s) 493
BmgT120I GGNCC 2 cut(s) 288, 363
BmiI GGNNCC 1 cut(s) 98
BmsI GCATC 1 cut(s) 329
BplI GAGNNNNNCTC 2 cut(s) 383, 415
Bpu10I CCTNAGC 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 313
BsaBI GATNNNNATC 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 92, 610
Bsc4I CCNNNNNNNGG 4 cut(s) 448, 577, 611, 692
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 2 cut(s) 745, 784
Bse8I GATNNNNATC 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 92, 610
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 4 cut(s) 448, 577, 611, 692
BseMI GCAATG 2 cut(s) 745, 784
BseMII CTCAG 4 cut(s) 96, 192, 306, 468
BseRI GAGGAG 2 cut(s) 407, 494
BseSI GKGCMC 1 cut(s) 101
BseXI GCAGC 4 cut(s) 184, 391, 394, 418
BseYI CCCAGC 2 cut(s) 25, 231
BsgI GTGCAG 1 cut(s) 151
BshFI GGCC 2 cut(s) 290, 678
BshNI GGYRCC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 493
BslI CCNNNNNNNGG 4 cut(s) 448, 577, 611, 692
BsmAI GTCTC 1 cut(s) 476
BsnI GGCC 2 cut(s) 290, 678
BsoBI CYCGRG 1 cut(s) 493
Bsp1286I GDGCHC 3 cut(s) 101, 211, 401
Bsp143I GATC 7 cut(s) 124, 135, 298, 412, 438, 466, 513
Bsp19I CCATGG 1 cut(s) 610
BspACI CCGC 2 cut(s) 708, 715
BspANI GGCC 2 cut(s) 290, 678
BspCNI CTCAG 4 cut(s) 97, 193, 307, 469
BspLI GGNNCC 1 cut(s) 98
BspPI GGATC 3 cut(s) 143, 306, 433
BspQI GCTCTTC 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 96
BspTI CTTAAG 2 cut(s) 182, 308
BsrBI CCGCTC 1 cut(s) 708
BsrDI GCAATG 2 cut(s) 745, 784
BssECI CCNNGG 2 cut(s) 92, 610
BssMI GATC 7 cut(s) 124, 135, 298, 412, 438, 466, 513
BssT1I CCWWGG 2 cut(s) 92, 610
Bst6I CTCTTC 1 cut(s) 195
BstAFI CTTAAG 2 cut(s) 182, 308
BstC8I GCNNGC 2 cut(s) 324, 500
BstDEI CTNAG 8 cut(s) 105, 201, 243, 284, 315, 477, 687, 753
BstDSI CCRYGG 1 cut(s) 610
BstENI CCTNNNNNAGG 1 cut(s) 575
BstKTI GATC 7 cut(s) 127, 138, 301, 415, 441, 469, 516
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 7 cut(s) 124, 135, 298, 412, 438, 466, 513
BstMWI GCNNNNNNNGC 3 cut(s) 194, 206, 714
BstSLI GKGCMC 1 cut(s) 101
BstV1I GCAGC 4 cut(s) 184, 391, 394, 418
BstX2I RGATCY 2 cut(s) 135, 438
BstYI RGATCY 2 cut(s) 135, 438
Bsu36I CCTNAGG 1 cut(s) 687
BsuRI GGCC 2 cut(s) 290, 678
BtgI CCRYGG 1 cut(s) 610
BtsIMutI CAGTG 2 cut(s) 134, 213
Cac8I GCNNGC 2 cut(s) 324, 500
CaiI CAGNNNCTG 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 288, 363
CviAII CATG 4 cut(s) 44, 406, 611, 764
DdeI CTNAG 8 cut(s) 105, 201, 243, 284, 315, 477, 687, 753
DpnI GATC 7 cut(s) 126, 137, 300, 414, 440, 468, 515
DpnII GATC 7 cut(s) 124, 135, 298, 412, 438, 466, 513
DraI TTTAAA 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 704
Ecl136II GAGCTC 1 cut(s) 209
Eco130I CCWWGG 2 cut(s) 92, 610
Eco24I GRGCYC 2 cut(s) 211, 401
Eco47I GGWCC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 491
Eco81I CCTNAGG 1 cut(s) 687
Eco88I CYCGRG 1 cut(s) 493
EcoICRI GAGCTC 1 cut(s) 209
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 288
EcoRI GAATTC 1 cut(s) 59
EcoT14I CCWWGG 2 cut(s) 92, 610
EcoT38I GRGCYC 2 cut(s) 211, 401
ErhI CCWWGG 2 cut(s) 92, 610
FaeI CATG 4 cut(s) 47, 409, 614, 767
FalI AAGNNNNNCTT 4 cut(s) 85, 117, 626, 658
FatI CATG 4 cut(s) 43, 405, 610, 763
Fnu4HI GCNGC 5 cut(s) 198, 380, 383, 432, 709
FriOI GRGCYC 2 cut(s) 211, 401
Fsp4HI GCNGC 5 cut(s) 198, 380, 383, 432, 709
FspBI CTAG 1 cut(s) 50
GluI GCNGC 5 cut(s) 198, 380, 383, 432, 709
GsaI CCCAGC 2 cut(s) 29, 235
HaeIII GGCC 2 cut(s) 290, 678
Hin1II CATG 4 cut(s) 47, 409, 614, 767
HinfI GANTC 3 cut(s) 257, 585, 601
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 3 cut(s) 106, 417, 471
Hpy188III TCNNGA 5 cut(s) 122, 442, 493, 619, 643
Hpy8I GTNNAC 1 cut(s) 526
HpyAV CCTTC 5 cut(s) 111, 271, 280, 466, 668
HpyCH4IV ACGT 1 cut(s) 682
HpyCH4V TGCA 9 cut(s) 43, 85, 132, 154, 250, 379, 564, 738, 767
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 206, 714
HpyF3I CTNAG 8 cut(s) 105, 201, 243, 284, 315, 477, 687, 753
HpySE526I ACGT 1 cut(s) 682
Hsp92II CATG 4 cut(s) 47, 409, 614, 767
Kzo9I GATC 7 cut(s) 124, 135, 298, 412, 438, 466, 513
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 1 cut(s) 705
LpnPI CCDG 6 cut(s) 39, 118, 245, 371, 455, 611
Lsp1109I GCAGC 4 cut(s) 184, 391, 394, 418
LweI GCATC 1 cut(s) 329
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 682
MalI GATC 7 cut(s) 126, 137, 300, 414, 440, 468, 515
MbiI CCGCTC 1 cut(s) 708
MboI GATC 7 cut(s) 124, 135, 298, 412, 438, 466, 513
MboII GAAGA 5 cut(s) 110, 130, 182, 523, 854
MflI RGATCY 2 cut(s) 135, 438
MhlI GDGCHC 3 cut(s) 101, 211, 401
MluCI AATT 9 cut(s) 59, 69, 146, 218, 446, 629, 722, 820, 849
MlyI GAGTC 1 cut(s) 266
MseI TTAA 3 cut(s) 183, 276, 309
MspA1I CMGCKG 1 cut(s) 385
MspCI CTTAAG 2 cut(s) 182, 308
MwoI GCNNNNNNNGC 3 cut(s) 194, 206, 714
NcoI CCATGG 1 cut(s) 610
NdeII GATC 7 cut(s) 124, 135, 298, 412, 438, 466, 513
NlaIII CATG 4 cut(s) 47, 409, 614, 767
NlaIV GGNNCC 1 cut(s) 98
NmeAIII GCCGAG 1 cut(s) 460
PaeR7I CTCGAG 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 585, 601
PflMI CCANNNNNTGG 1 cut(s) 448
PkrI GCNGC 5 cut(s) 199, 381, 384, 433, 710
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PsiI TTATAA 1 cut(s) 747
Psp124BI GAGCTC 1 cut(s) 211
PspFI CCCAGC 2 cut(s) 25, 231
PspN4I GGNNCC 1 cut(s) 98
PspPI GGNCC 2 cut(s) 288, 363
PstNI CAGNNNCTG 1 cut(s) 385
PsuI RGATCY 2 cut(s) 135, 438
PvuII CAGCTG 1 cut(s) 385
SacI GAGCTC 1 cut(s) 211
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 3 cut(s) 183, 276, 309
SatI GCNGC 5 cut(s) 198, 380, 383, 432, 709
Sau3AI GATC 7 cut(s) 124, 135, 298, 412, 438, 466, 513
Sau96I GGNCC 2 cut(s) 288, 363
SchI GAGTC 1 cut(s) 266
SduI GDGCHC 3 cut(s) 101, 211, 401
SfaNI GCATC 1 cut(s) 329
Sfr274I CTCGAG 1 cut(s) 493
SinI GGWCC 1 cut(s) 363
SlaI CTCGAG 1 cut(s) 493
SmlI CTYRAG 4 cut(s) 182, 292, 308, 493
SmoI CTYRAG 4 cut(s) 182, 292, 308, 493
SpeI ACTAGT 1 cut(s) 49
Sse9I AATT 9 cut(s) 59, 69, 146, 218, 446, 629, 722, 820, 849
SsiI CCGC 2 cut(s) 708, 715
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 211
StyI CCWWGG 2 cut(s) 92, 610
TaiI ACGT 1 cut(s) 685
TaqI TCGA 4 cut(s) 401, 494, 516, 588
TasI AATT 9 cut(s) 59, 69, 146, 218, 446, 629, 722, 820, 849
TauI GCSGC 1 cut(s) 711
TfiI GAWTC 2 cut(s) 585, 601
Tru1I TTAA 3 cut(s) 183, 276, 309
Tru9I TTAA 3 cut(s) 183, 276, 309
TscAI CASTG 2 cut(s) 134, 220
TseI GCWGC 4 cut(s) 197, 379, 382, 431
TspDTI ATGAA 4 cut(s) 60, 253, 422, 752
TspRI CASTG 2 cut(s) 134, 220
Van91I CCANNNNNTGG 1 cut(s) 448
Vha464I CTTAAG 2 cut(s) 182, 308
VpaK11BI GGWCC 1 cut(s) 363
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 4 cut(s) 59, 146, 722, 820
XhoI CTCGAG 1 cut(s) 493
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.