pycom14g00350

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015701
Physical Location & Seq
Forward (+)
228557 .. 229694
1138 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g00350.2

Sequence Viewer

Length: 729 bp
ATGCATCACTTGGTGGCGTTGACTCCATTTGTACCCACACTGAGAATTTATTGTAACAAACCGCGACTATCTTTTCATCTATGTATTACTAAGGAGAGCAAGGGTCTTGTAGCTAGCCTAACTGATTTTCACATGGATTCCAATGGTGAATATGAGACAGAAGCTGAGACCATGGCTCTTCGTCGGTCAAGATCTTTTGATTCTGTTCCTGCTGAGGTTGCCACTGCTGTTGCTGCTTCTTGTTGCGCTGGTGTGATGCCTCCTCCTTTGGCTTTTCTTCCAGCCACGACCTATTTGCCTGAACTAGTTAGAGAGTTCAGGCAAATTATGACAAAGCTGAGATCCACAAGGCGCCTTTCTGAGCCTCAACATCTTCAAGATCAGCGTATGATCTTCAGAGAATGGATGCAGAGGGACTTGTCTGCCTTCTTTAGCCTTAAGGCTCTTCAAGACGTTGAGATGGCTCTCACTAAATTATACCAAGCCCAGCAGATGACTAAGGTGCAATATGAGTCCTTCGTTTCCTTTTTTGAAAATCTCAGGGCCTTGAGGGATCGACATCTTAAGGCCGAGAGGCAAGCCAACAGGGTGAGGTGCTACAAGGAGAAGCACACTCGGACCTCCACTGCTCTGCAGCAGTTGGTGGATGAGGGCTCGTCCATGGAGGATCGGATAATAGTGGTAGCTGTCGATCGATCCAGAAATTGGAGGAACAACTTTCTGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
HSP20

Protein Analysis

243

Amino Acids

28.11

Weight (kDa)

9.3

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015701)

Species Orthologous Gene IDs
malus_domestica MD14G1005100.v1.1 MD14G1005200.v1.1
prunus_persica Prupe.7G008400_v2.0.a1
pyrus_communis pycom14g00340 pycom14g00350 pycom14g00360 pycom14g00370
rosa_chinensis RchiOBHm_Chr3g0496541
rosa_laevigata RLG00000022520
rosa_multiflora Rmu_sc0000741.1_g000008
rosa_roxburghii Rroxscaffold_6G00388950
rosa_rugosa Rorug03G0283900
rosa_samantha Rh3BG365300 Rh3CG362600 Rh3DG365700
rosa_wichuraiana Rw3G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 705
AccII CGCG 1 cut(s) 64
AciI CCGC 1 cut(s) 62
AclWI GGATC 4 cut(s) 336, 561, 675, 690
AcsI RAATTY 1 cut(s) 45
AcuI CTGAAG 1 cut(s) 379
AcyI GRCGYC 1 cut(s) 352
AdeI CACNNNGTG 1 cut(s) 13
AfaI GTAC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 705
AflII CTTAAG 2 cut(s) 437, 563
AgsI TTSAA 3 cut(s) 377, 449, 533
AhlI ACTAGT 1 cut(s) 304
AluBI AGCT 4 cut(s) 113, 164, 337, 686
AluI AGCT 4 cut(s) 113, 164, 337, 686
Alw26I GTCTC 2 cut(s) 149, 161
AlwI GGATC 4 cut(s) 336, 561, 675, 690
AlwNI CAGNNNCTG 1 cut(s) 164
AoxI GGCC 2 cut(s) 543, 567
ApeKI GCWGC 2 cut(s) 233, 634
ApoI RAATTY 1 cut(s) 45
AspLEI GCGC 2 cut(s) 248, 354
AspS9I GGNCC 2 cut(s) 543, 618
AsuHPI GGTGA 2 cut(s) 158, 601
AsuNHI GCTAGC 1 cut(s) 113
AvaII GGWCC 1 cut(s) 618
BanI GGYRCC 1 cut(s) 351
BanII GRGCYC 1 cut(s) 656
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 220, 646
BccI CCATC 1 cut(s) 454
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BcoDI GTCTC 2 cut(s) 149, 161
BcuI ACTAGT 1 cut(s) 304
BfaI CTAG 2 cut(s) 114, 305
BfmI CTRYAG 1 cut(s) 632
BfoI RGCGCY 1 cut(s) 355
BfrI CTTAAG 2 cut(s) 437, 563
BglII AGATCT 1 cut(s) 191
BisI GCNGC 2 cut(s) 234, 635
BlsI GCNGC 2 cut(s) 235, 636
Bme18I GGWCC 1 cut(s) 618
BmgT120I GGNCC 2 cut(s) 543, 618
BmiI GGNNCC 1 cut(s) 353
BmsI GCATC 3 cut(s) 13, 246, 396
BmtI GCTAGC 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 568
Bsa29I ATCGAT 1 cut(s) 694
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 352
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 2 cut(s) 171, 660
Bsc4I CCNNNNNNNGG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 558
BseCI ATCGAT 1 cut(s) 694
BseDI CCNNGG 2 cut(s) 171, 660
BseGI GGATG 2 cut(s) 411, 652
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 1 cut(s) 705
BseMII CTCAG 6 cut(s) 32, 156, 204, 329, 351, 553
BseRI GAGGAG 1 cut(s) 252
BseXI GCAGC 2 cut(s) 220, 646
BseYI CCCAGC 1 cut(s) 486
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 2 cut(s) 545, 569
BshNI GGYRCC 1 cut(s) 351
BshVI ATCGAT 1 cut(s) 694
BsiEI CGRYCG 1 cut(s) 694
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 1 cut(s) 705
BsmAI GTCTC 2 cut(s) 149, 161
BsmFI GGGAC 1 cut(s) 428
BsnI GGCC 2 cut(s) 545, 569
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 656
Bsp143I GATC 8 cut(s) 191, 341, 379, 390, 553, 667, 691, 695
Bsp19I CCATGG 2 cut(s) 171, 660
BspACI CCGC 1 cut(s) 62
BspANI GGCC 2 cut(s) 545, 569
BspCNI CTCAG 6 cut(s) 33, 157, 205, 330, 352, 552
BspDI ATCGAT 1 cut(s) 694
BspFNI CGCG 1 cut(s) 64
BspLI GGNNCC 1 cut(s) 353
BspMAI CTGCAG 1 cut(s) 636
BspOI GCTAGC 1 cut(s) 117
BspPI GGATC 4 cut(s) 336, 561, 675, 690
BspQI GCTCTTC 2 cut(s) 183, 450
BspT107I GGYRCC 1 cut(s) 351
BspTI CTTAAG 2 cut(s) 437, 563
BspTNI GGTCTC 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 171, 660
BssMI GATC 8 cut(s) 191, 341, 379, 390, 553, 667, 691, 695
BssNI GRCGYC 1 cut(s) 352
BssT1I CCWWGG 2 cut(s) 171, 660
Bst6I CTCTTC 2 cut(s) 183, 450
BstACI GRCGYC 1 cut(s) 352
BstAFI CTTAAG 2 cut(s) 437, 563
BstC8I GCNNGC 2 cut(s) 115, 579
BstDEI CTNAG 8 cut(s) 41, 90, 165, 213, 338, 360, 498, 539
BstDSI CCRYGG 2 cut(s) 171, 660
BstF5I GGATG 2 cut(s) 411, 652
BstFNI CGCG 1 cut(s) 64
BstH2I RGCGCY 1 cut(s) 355
BstHHI GCGC 2 cut(s) 248, 354
BstKTI GATC 8 cut(s) 194, 344, 382, 393, 556, 670, 694, 698
BstMAI GTCTC 2 cut(s) 149, 161
BstMBI GATC 8 cut(s) 191, 341, 379, 390, 553, 667, 691, 695
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 2 cut(s) 218, 233
BstSFI CTRYAG 1 cut(s) 632
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 2 cut(s) 220, 646
BstX2I RGATCY 2 cut(s) 191, 341
BstYI RGATCY 2 cut(s) 191, 341
Bsu15I ATCGAT 1 cut(s) 694
BsuRI GGCC 2 cut(s) 545, 569
BsuTUI ATCGAT 1 cut(s) 694
BtgI CCRYGG 2 cut(s) 171, 660
BtsCI GGATG 2 cut(s) 411, 652
BtsI GCAGTG 2 cut(s) 222, 624
BtsIMutI CAGTG 3 cut(s) 38, 222, 624
Cac8I GCNNGC 2 cut(s) 115, 579
CaiI CAGNNNCTG 1 cut(s) 164
CfoI GCGC 2 cut(s) 248, 354
Cfr13I GGNCC 2 cut(s) 543, 618
ClaI ATCGAT 1 cut(s) 694
Csp6I GTAC 1 cut(s) 32
CspCI CAANNNNNGTGG 2 cut(s) 211, 246
CviAII CATG 3 cut(s) 133, 172, 661
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 8 cut(s) 41, 90, 165, 213, 338, 360, 498, 539
DinI GGCGCC 1 cut(s) 353
DpnI GATC 8 cut(s) 193, 343, 381, 392, 555, 669, 693, 697
DpnII GATC 8 cut(s) 191, 341, 379, 390, 553, 667, 691, 695
DraIII CACNNNGTG 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 183, 450
EarI CTCTTC 2 cut(s) 183, 450
Eco130I CCWWGG 2 cut(s) 171, 660
Eco24I GRGCYC 1 cut(s) 656
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 618
Eco57I CTGAAG 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 543
EcoT14I CCWWGG 2 cut(s) 171, 660
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 656
EgeI GGCGCC 1 cut(s) 353
EheI GGCGCC 1 cut(s) 353
ErhI CCWWGG 2 cut(s) 171, 660
FaeI CATG 3 cut(s) 136, 175, 664
FaiI YATR 9 cut(s) 82, 134, 153, 173, 329, 389, 478, 510, 662
FaqI GGGAC 1 cut(s) 428
FatI CATG 3 cut(s) 132, 171, 660
Fnu4HI GCNGC 2 cut(s) 234, 635
FokI GGATG 2 cut(s) 418, 659
FriOI GRGCYC 1 cut(s) 656
Fsp4HI GCNGC 2 cut(s) 234, 635
FspBI CTAG 2 cut(s) 114, 305
GlaI GCGC 2 cut(s) 247, 353
GluI GCNGC 2 cut(s) 234, 635
GsaI CCCAGC 1 cut(s) 490
HaeII RGCGCY 1 cut(s) 355
HaeIII GGCC 2 cut(s) 545, 569
HhaI GCGC 2 cut(s) 248, 354
Hin1I GRCGYC 1 cut(s) 352
Hin1II CATG 3 cut(s) 136, 175, 664
Hin6I GCGC 2 cut(s) 246, 352
HinP1I GCGC 2 cut(s) 246, 352
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 4 cut(s) 22, 137, 200, 512
HphI GGTGA 2 cut(s) 158, 601
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 4 cut(s) 361, 398, 618, 672
Hpy188III TCNNGA 4 cut(s) 189, 377, 449, 699
Hpy8I GTNNAC 1 cut(s) 21
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 2 cut(s) 436, 526
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 4 cut(s) 4, 409, 505, 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 233
HpyF3I CTNAG 8 cut(s) 41, 90, 165, 213, 338, 360, 498, 539
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 1 cut(s) 352
Hsp92II CATG 3 cut(s) 136, 175, 664
HspAI GCGC 2 cut(s) 246, 352
KasI GGCGCC 1 cut(s) 351
Kzo9I GATC 8 cut(s) 191, 341, 379, 390, 553, 667, 691, 695
LguI GCTCTTC 2 cut(s) 183, 450
LpnPI CCDG 9 cut(s) 222, 234, 294, 304, 312, 500, 526, 571, 712
Lsp1109I GCAGC 2 cut(s) 220, 646
LweI GCATC 3 cut(s) 13, 246, 396
MaeI CTAG 2 cut(s) 114, 305
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 53
MalI GATC 8 cut(s) 193, 343, 381, 392, 555, 669, 693, 697
MboI GATC 8 cut(s) 191, 341, 379, 390, 553, 667, 691, 695
MboII GAAGA 5 cut(s) 170, 269, 365, 385, 437
MflI RGATCY 2 cut(s) 191, 341
MhlI GDGCHC 1 cut(s) 656
MluCI AATT 4 cut(s) 45, 324, 473, 703
Mly113I GGCGCC 1 cut(s) 352
MlyI GAGTC 2 cut(s) 16, 521
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 438, 564
MspCI CTTAAG 2 cut(s) 437, 563
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 2 cut(s) 218, 233
NarI GGCGCC 1 cut(s) 352
NcoI CCATGG 2 cut(s) 171, 660
NdeII GATC 8 cut(s) 191, 341, 379, 390, 553, 667, 691, 695
NheI GCTAGC 1 cut(s) 113
NlaIII CATG 3 cut(s) 136, 175, 664
NlaIV GGNNCC 1 cut(s) 353
NmeAIII GCCGAG 1 cut(s) 595
NsiI ATGCAT 1 cut(s) 6
PciSI GCTCTTC 2 cut(s) 183, 450
PfeI GAWTC 2 cut(s) 137, 200
PflMI CCANNNNNTGG 1 cut(s) 705
PkrI GCNGC 2 cut(s) 235, 636
Ple19I CGATCG 1 cut(s) 694
PleI GAGTC 2 cut(s) 16, 520
PluTI GGCGCC 1 cut(s) 355
PpsI GAGTC 2 cut(s) 16, 520
PspFI CCCAGC 1 cut(s) 486
PspN4I GGNNCC 1 cut(s) 353
PspPI GGNCC 2 cut(s) 543, 618
PstI CTGCAG 1 cut(s) 636
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 2 cut(s) 191, 341
PvuI CGATCG 1 cut(s) 694
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
SapI GCTCTTC 2 cut(s) 183, 450
SaqAI TTAA 2 cut(s) 438, 564
SatI GCNGC 2 cut(s) 234, 635
Sau3AI GATC 8 cut(s) 191, 341, 379, 390, 553, 667, 691, 695
Sau96I GGNCC 2 cut(s) 543, 618
SchI GAGTC 2 cut(s) 16, 521
SduI GDGCHC 1 cut(s) 656
SfaNI GCATC 3 cut(s) 13, 246, 396
SfcI CTRYAG 1 cut(s) 632
SfoI GGCGCC 1 cut(s) 353
SinI GGWCC 1 cut(s) 618
SmlI CTYRAG 3 cut(s) 437, 547, 563
SmoI CTYRAG 3 cut(s) 437, 547, 563
SpeI ACTAGT 1 cut(s) 304
Sse9I AATT 4 cut(s) 45, 324, 473, 703
SsiI CCGC 1 cut(s) 62
SspDI GGCGCC 1 cut(s) 351
SspMI CTAG 2 cut(s) 114, 305
StyI CCWWGG 2 cut(s) 171, 660
TaiI ACGT 1 cut(s) 456
TaqI TCGA 3 cut(s) 556, 690, 694
TaqII GACCGA 1 cut(s) 174
TasI AATT 4 cut(s) 45, 324, 473, 703
TfiI GAWTC 2 cut(s) 137, 200
Tru1I TTAA 2 cut(s) 438, 564
Tru9I TTAA 2 cut(s) 438, 564
TscAI CASTG 3 cut(s) 45, 229, 631
TseI GCWGC 2 cut(s) 233, 634
TspDTI ATGAA 1 cut(s) 65
TspRI CASTG 3 cut(s) 45, 229, 631
Van91I CCANNNNNTGG 1 cut(s) 705
Vha464I CTTAAG 2 cut(s) 437, 563
VpaK11BI GGWCC 1 cut(s) 618
XapI RAATTY 1 cut(s) 45
XspI CTAG 2 cut(s) 114, 305
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.