MD14G1042600.v1.1
MYB Family

Myb/SANT-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002174
Physical Location & Seq
Reverse (-)
4048675 .. 4051929
3255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1042600.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGTATGCCGGTTCTTCTACCACCAGTGACCATGTAAGGGCTAACTGGACTCCATCACAAGACAATTTTTTCATTGACCTTATGCTAGAACAAGTGAGCAAAGGGAACAAAACCGGTCATGGATTTAGGAAACAAGCTTGGGCAGATATGATTGTACTCTTTAATAATGAATTTGGGTTTAAGTATGATACAGATATTCTGAAAAATCGATACAAAAGATTGAGGAAGCAGTACAGCGAGATGAAGAGTCTTGTTGATCAGGGAAACTTTAGATGGGATGAAACGCAACAGATGATAACAGCTGACGATAATGTGTGGAATAATTATATCAAGGCCCACCCTGAAATGCAACCGTACAGAACAAAAGTCATTTCGCATTATGACGAGTTGTGCATAATATGTGGCCATGCAGTTGCTGATGGAAGATACAGTCTTTCATGTTACGATATGGACTTTGAGAACGAAGCAAGAGGAATGGAATTTCAAGATCCCACTGATGCTGATCCTAAAAAAATTGACTGGTCACAGATTATGGACCAATTTTTCGTTGAACTAATGTTGGATGAAGTGCGCAAAGGGAATAAGGTTGGTTGCACTTTCAAGAAGAAATCATGGATTACCATGATCACATGCTTTAATGAGAAATTCGGGTTTCAGCATGGTAGGGCTGTATTGAAAAATCGGTACAATGTCCTCAGGAAACATTACAGTAGCGTTAACGCCCTGCTTGATCAAAAGGGTTTTACTTGGGATGAAACACAACAAAAGGTGGTAGCTGATGATCCGGTTTGGAACAAATATATAGAGGCACATCCCAGTTTCCACATGTACAGAAATAAAGCTATGCCATATTATTCCGATTTGTGTGTCATATGCGGAAATGAGGCTAGACTTTTGAAAGAAGTTCCATCAAGCTGCAATGAAGCTCTTGACAAGGGAACCTTTGCTAAGAAAATTGACGGAGATGCTACACCTATTGTTGACAAACAAAATGTTCAGAAAAAAGCATTGGATTCAAGTGATGTCAAGAATTTATCAGATCAAAAGGAAAGGCACAGTCACGATGTCACACAAACCTTACACCAGTCTAAGAAACCACAAAGAACGGATGGAGTTGCGGCCAATGCTCTAGGAGATATGGCAGTTGCAGAGTCACCGTTGAAGAAGAAACTGAAGAAAGAGGCATCTGTATCCATAGAGAAAGTTATTGATGTCTTAAAGGCTATACCGGACATTGATGATGATTTACTACTGGATGCCATTGACCTTTTGGAGGATGAGAAGAGAGCCAGGATGTTTTTAGCATTGCACCCCGCTCTGCGAAAGAAGTGGTTGATGAGAAAGCTTCGTCCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-bind_3

Protein Analysis

453

Amino Acids

52.28

Weight (kDa)

7.97

Isoelectric Point (pI)

29.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 15 - 109 1.4e-31 Myb/SANT-like DNA-binding domain
Myb_DNA-bind_3 PF12776 174 - 267 5.5e-27 Myb/SANT-like DNA-binding domain
At2g29880_C PF24769 403 - 448 1.3e-06 At2g29880 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 572
AccBSI CCGCTC 1 cut(s) 1316
AciI CCGC 3 cut(s) 876, 1118, 1314
AclWI GGATC 3 cut(s) 482, 497, 776
AcoI YGGCCR 2 cut(s) 403, 1119
AcsI RAATTY 4 cut(s) 170, 479, 644, 1030
AcuI CTGAAG 1 cut(s) 1193
AfaI GTAC 5 cut(s) 156, 233, 356, 686, 830
AfiI CCNNNNNNNGG 2 cut(s) 37, 1273
AflIII ACRYGT 1 cut(s) 825
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 7 cut(s) 485, 551, 601, 676, 898, 1017, 1162
AjnI CCWGG 1 cut(s) 1289
AjuI GAANNNNNNNTTGG 2 cut(s) 992, 1024
AluBI AGCT 7 cut(s) 137, 302, 776, 842, 915, 926, 1345
AluI AGCT 7 cut(s) 137, 302, 776, 842, 915, 926, 1345
AlwI GGATC 3 cut(s) 482, 497, 776
AlwNI CAGNNNCTG 1 cut(s) 416
AoxI GGCC 3 cut(s) 333, 403, 1119
ApeKI GCWGC 1 cut(s) 915
ApoI RAATTY 4 cut(s) 170, 479, 644, 1030
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 573
AspS9I GGNCC 2 cut(s) 334, 535
AsuHPI GGTGA 1 cut(s) 1146
AvaII GGWCC 1 cut(s) 535
AxyI CCTNAGG 1 cut(s) 695
BalI TGGCCA 1 cut(s) 405
BbvI GCAGC 1 cut(s) 902
BccI CCATC 5 cut(s) 61, 267, 413, 916, 1103
BciT130I CCWGG 1 cut(s) 1291
BciVI GTATCC 1 cut(s) 1201
BclI TGATCA 3 cut(s) 256, 624, 730
BfaI CTAG 3 cut(s) 86, 888, 1130
BfuI GTATCC 1 cut(s) 1201
BisI GCNGC 2 cut(s) 916, 1119
BlsI GCNGC 2 cut(s) 917, 1120
Bme1390I CCNGG 1 cut(s) 1291
Bme18I GGWCC 1 cut(s) 535
BmgT120I GGNCC 2 cut(s) 334, 535
BmiI GGNNCC 1 cut(s) 940
BmrFI CCNGG 1 cut(s) 1291
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 4 cut(s) 487, 955, 1193, 1246
BmuI ACTGGG 1 cut(s) 810
Bsa29I ATCGAT 1 cut(s) 208
BsaBI GATNNNNATC 1 cut(s) 501
BsaWI WCCGGW 3 cut(s) 113, 784, 1228
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 1273
Bse118I RCCGGY 2 cut(s) 8, 113
Bse1I ACTGG 6 cut(s) 24, 50, 524, 816, 1084, 1257
Bse21I CCTNAGG 1 cut(s) 695
Bse3DI GCAATG 2 cut(s) 925, 1304
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 1 cut(s) 1291
BseCI ATCGAT 1 cut(s) 208
BseGI GGATG 8 cut(s) 283, 568, 757, 811, 1114, 1261, 1282, 1299
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 37, 1273
BseMI GCAATG 2 cut(s) 925, 1304
BseMII CTCAG 1 cut(s) 709
BseNI ACTGG 6 cut(s) 24, 50, 524, 816, 1084, 1257
BseXI GCAGC 1 cut(s) 902
BshFI GGCC 3 cut(s) 335, 405, 1121
BshTI ACCGGT 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 208
BsiSI CCGG 4 cut(s) 9, 114, 785, 1229
BslI CCNNNNNNNGG 2 cut(s) 37, 1273
BsnI GGCC 3 cut(s) 335, 405, 1121
Bsp1407I TGTACA 1 cut(s) 828
Bsp143I GATC 7 cut(s) 256, 487, 502, 624, 730, 781, 1039
BspACI CCGC 3 cut(s) 876, 1118, 1314
BspANI GGCC 3 cut(s) 335, 405, 1121
BspCNI CTCAG 1 cut(s) 708
BspDI ATCGAT 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 940
BspPI GGATC 3 cut(s) 482, 497, 776
BsrBI CCGCTC 1 cut(s) 1316
BsrDI GCAATG 2 cut(s) 925, 1304
BsrFI RCCGGY 2 cut(s) 8, 113
BsrGI TGTACA 1 cut(s) 828
BsrI ACTGG 6 cut(s) 24, 50, 524, 816, 1084, 1257
BssAI RCCGGY 2 cut(s) 8, 113
BssMI GATC 7 cut(s) 256, 487, 502, 624, 730, 781, 1039
Bst2UI CCWGG 1 cut(s) 1291
Bst4CI ACNGT 6 cut(s) 354, 431, 710, 1058, 1158, 1356
Bst6I CTCTTC 2 cut(s) 239, 1277
BstAUI TGTACA 1 cut(s) 828
BstDEI CTNAG 3 cut(s) 695, 948, 1089
BstENI CCTNNNNNAGG 1 cut(s) 1271
BstF5I GGATG 8 cut(s) 283, 568, 757, 811, 1114, 1261, 1282, 1299
BstHHI GCGC 1 cut(s) 573
BstKTI GATC 7 cut(s) 259, 490, 505, 627, 733, 784, 1042
BstMBI GATC 7 cut(s) 256, 487, 502, 624, 730, 781, 1039
BstMWI GCNNNNNNNGC 1 cut(s) 1124
BstNI CCWGG 1 cut(s) 1291
BstNSI RCATGY 2 cut(s) 633, 829
BstSCI CCNGG 1 cut(s) 1289
BstV1I GCAGC 1 cut(s) 902
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
Bsu15I ATCGAT 1 cut(s) 208
Bsu36I CCTNAGG 1 cut(s) 695
BsuI GTATCC 1 cut(s) 1201
BsuRI GGCC 3 cut(s) 335, 405, 1121
BsuTUI ATCGAT 1 cut(s) 208
BtsCI GGATG 8 cut(s) 283, 568, 757, 811, 1114, 1261, 1282, 1299
BtsIMutI CAGTG 2 cut(s) 31, 492
CaiI CAGNNNCTG 1 cut(s) 416
CfoI GCGC 1 cut(s) 573
Cfr10I RCCGGY 2 cut(s) 8, 113
Cfr13I GGNCC 2 cut(s) 334, 535
ClaI ATCGAT 1 cut(s) 208
Csp6I GTAC 5 cut(s) 155, 232, 355, 685, 829
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 9 cut(s) 32, 119, 407, 438, 612, 622, 630, 659, 826
CviQI GTAC 5 cut(s) 155, 232, 355, 685, 829
DdeI CTNAG 3 cut(s) 695, 948, 1089
DpnI GATC 7 cut(s) 258, 489, 504, 626, 732, 783, 1041
DpnII GATC 7 cut(s) 256, 487, 502, 624, 730, 781, 1039
EaeI YGGCCR 2 cut(s) 403, 1119
Eam1104I CTCTTC 2 cut(s) 239, 1277
EarI CTCTTC 2 cut(s) 239, 1277
Eco47I GGWCC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 1193
Eco81I CCTNAGG 1 cut(s) 695
EcoNI CCTNNNNNAGG 1 cut(s) 1271
EcoRII CCWGG 1 cut(s) 1289
FaeI CATG 9 cut(s) 35, 122, 410, 441, 615, 625, 633, 662, 829
FalI AAGNNNNNCTT 2 cut(s) 926, 958
FatI CATG 9 cut(s) 31, 118, 406, 437, 611, 621, 629, 658, 825
FauI CCCGC 1 cut(s) 1321
FauNDI CATATG 1 cut(s) 872
FbaI TGATCA 3 cut(s) 256, 624, 730
Fnu4HI GCNGC 2 cut(s) 916, 1119
FokI GGATG 8 cut(s) 290, 575, 764, 798, 1121, 1268, 1289, 1306
Fsp4HI GCNGC 2 cut(s) 916, 1119
FspBI CTAG 3 cut(s) 86, 888, 1130
FspI TGCGCA 1 cut(s) 572
GlaI GCGC 1 cut(s) 572
GluI GCNGC 2 cut(s) 916, 1119
HaeIII GGCC 3 cut(s) 335, 405, 1121
HapII CCGG 4 cut(s) 9, 114, 785, 1229
HhaI GCGC 1 cut(s) 573
Hin1II CATG 9 cut(s) 35, 122, 410, 441, 615, 625, 633, 662, 829
Hin6I GCGC 1 cut(s) 571
HinP1I GCGC 1 cut(s) 571
HincII GTYRAC 2 cut(s) 718, 982
HindII GTYRAC 2 cut(s) 718, 982
HindIII AAGCTT 2 cut(s) 135, 1343
HinfI GANTC 4 cut(s) 49, 247, 1013, 1151
HpaI GTTAAC 1 cut(s) 718
HpaII CCGG 4 cut(s) 9, 114, 785, 1229
HphI GGTGA 1 cut(s) 1146
Hpy166II GTNNAC 3 cut(s) 718, 982, 1352
Hpy188I TCNGA 4 cut(s) 201, 859, 999, 1039
Hpy188III TCNNGA 6 cut(s) 485, 601, 697, 929, 1027, 1061
Hpy8I GTNNAC 3 cut(s) 718, 982, 1352
HpyCH4III ACNGT 6 cut(s) 354, 431, 710, 1058, 1158, 1356
HpyCH4V TGCA 7 cut(s) 349, 393, 410, 594, 918, 1148, 1309
HpyF10VI GCNNNNNNNGC 1 cut(s) 1124
HpyF3I CTNAG 3 cut(s) 695, 948, 1089
Hsp92II CATG 9 cut(s) 35, 122, 410, 441, 615, 625, 633, 662, 829
HspAI GCGC 1 cut(s) 571
Ksp22I TGATCA 3 cut(s) 256, 624, 730
KspAI GTTAAC 1 cut(s) 718
Kzo9I GATC 7 cut(s) 256, 487, 502, 624, 730, 781, 1039
Lsp1109I GCAGC 1 cut(s) 902
LweI GCATC 4 cut(s) 487, 955, 1193, 1246
MaeI CTAG 3 cut(s) 86, 888, 1130
MaeIII GTNAC 6 cut(s) 26, 440, 522, 1058, 1066, 1152
MalI GATC 7 cut(s) 258, 489, 504, 626, 732, 783, 1041
MbiI CCGCTC 1 cut(s) 1316
MboI GATC 7 cut(s) 256, 487, 502, 624, 730, 781, 1039
MboII GAAGA 8 cut(s) 6, 256, 435, 616, 1174, 1177, 1186, 1294
MflI RGATCY 1 cut(s) 487
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 9 cut(s) 64, 170, 322, 479, 513, 539, 644, 954, 1030
MluNI TGGCCA 1 cut(s) 405
MlyI GAGTC 3 cut(s) 43, 256, 1160
MmeI TCCRAC 1 cut(s) 540
MnlI CCTC 7 cut(s) 216, 464, 704, 799, 877, 1174, 1267
Mox20I TGGCCA 1 cut(s) 405
MscI TGGCCA 1 cut(s) 405
MseI TTAA 5 cut(s) 162, 180, 636, 717, 1217
Msp20I TGGCCA 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 4 cut(s) 9, 114, 785, 1229
MspR9I CCNGG 1 cut(s) 1291
MvaI CCWGG 1 cut(s) 1291
MwoI GCNNNNNNNGC 1 cut(s) 1124
NdeI CATATG 1 cut(s) 872
NdeII GATC 7 cut(s) 256, 487, 502, 624, 730, 781, 1039
NlaIII CATG 9 cut(s) 35, 122, 410, 441, 615, 625, 633, 662, 829
NlaIV GGNNCC 1 cut(s) 940
NmuCI GTSAC 5 cut(s) 26, 522, 1058, 1066, 1152
NsbI TGCGCA 1 cut(s) 572
NspI RCATGY 2 cut(s) 633, 829
PciI ACATGT 1 cut(s) 825
PfeI GAWTC 1 cut(s) 1013
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 2 cut(s) 917, 1120
PleI GAGTC 3 cut(s) 43, 255, 1159
PpsI GAGTC 3 cut(s) 43, 255, 1159
PscI ACATGT 1 cut(s) 825
Psp6I CCWGG 1 cut(s) 1289
PspGI CCWGG 1 cut(s) 1289
PspN4I GGNNCC 1 cut(s) 940
PspPI GGNCC 2 cut(s) 334, 535
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 487
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 5 cut(s) 156, 233, 356, 686, 830
RsaNI GTAC 5 cut(s) 155, 232, 355, 685, 829
SaqAI TTAA 5 cut(s) 162, 180, 636, 717, 1217
SatI GCNGC 2 cut(s) 916, 1119
Sau3AI GATC 7 cut(s) 256, 487, 502, 624, 730, 781, 1039
Sau96I GGNCC 2 cut(s) 334, 535
SchI GAGTC 3 cut(s) 43, 256, 1160
ScrFI CCNGG 1 cut(s) 1291
SfaNI GCATC 4 cut(s) 487, 955, 1193, 1246
SinI GGWCC 1 cut(s) 535
Sse9I AATT 9 cut(s) 64, 170, 322, 479, 513, 539, 644, 954, 1030
SsiI CCGC 3 cut(s) 876, 1118, 1314
SspMI CTAG 3 cut(s) 86, 888, 1130
StyD4I CCNGG 1 cut(s) 1289
TaaI ACNGT 6 cut(s) 354, 431, 710, 1058, 1158, 1356
TaqI TCGA 1 cut(s) 208
TasI AATT 9 cut(s) 64, 170, 322, 479, 513, 539, 644, 954, 1030
TatI WGTACW 3 cut(s) 154, 231, 828
TauI GCSGC 1 cut(s) 1121
TfiI GAWTC 1 cut(s) 1013
Tru1I TTAA 5 cut(s) 162, 180, 636, 717, 1217
Tru9I TTAA 5 cut(s) 162, 180, 636, 717, 1217
TscAI CASTG 2 cut(s) 31, 499
TseFI GTSAC 5 cut(s) 26, 522, 1058, 1066, 1152
TseI GCWGC 1 cut(s) 915
Tsp45I GTSAC 5 cut(s) 26, 522, 1058, 1066, 1152
TspDTI ATGAA 8 cut(s) 61, 183, 257, 294, 426, 579, 768, 936
TspGWI ACGGA 2 cut(s) 975, 1121
TspRI CASTG 2 cut(s) 31, 499
VpaK11BI GGWCC 1 cut(s) 535
XagI CCTNNNNNAGG 1 cut(s) 1271
XapI RAATTY 4 cut(s) 170, 479, 644, 1030
XceI RCATGY 2 cut(s) 633, 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 1267
XspI CTAG 3 cut(s) 86, 888, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.