MD14G1073200.v1.1

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0019119
Physical Location & Seq
Forward (+)
8139985 .. 8142010
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1073200.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGTGCATGGATTATAGAGCTCTAAATTTGATCACAATAAAGGATAAGTATCCAATACCCTTAATTGATGAATTGTTGGATGAGTTGTTTGGTGCTCAATTTTTTTCGAAGTTGGATTTGCGGGCAGGATATCATCAGATCAGGGTCCATCCTAGTGACATTGAGAAGACAGCATTTCGCACCCATGATGGTCACTATGAATTTTTATTTGTTTTGGTTTTCTTTGATGATATTCTCGTGTACAGTGACTCTTGGGAATCCCATCTTCACCATTTGTCTCTGGTTTTCAAAGTTCTCCAAGACCATCAATTGTTTGTCAAGCAAACTAAGTGTGACTTTGGCAAATCACAAATCGAATATTTGGGCCATGTGGTGTCTCGGCAAGGGGTGGCAGCAGATCCAAGTAAGCTTAAATCCATCCAAGATTGGCCTCTTCCTCATTCGGTTAGGGCTCTCAGGGGTTTTCTTGGCCTCACTGGCTATTATCGAAAATTCATTCTGAATTACGGAAAGATTTGTGGACCACTTACTGTACTAACAATAAAGGATGCATTCAAGTGGACTGATGAAGCTACTAGGGCCTTCCAGGCTCTCAAAGATCTCATGGTATCTCCTCATGTTCTTGCTTTACCAGATTTTTCCAAAACCTTCATTATTGAAACTGATGCTTCCAATAATGGACTTGGTGCTGTTTTACACCAAGAAGGGAGGCCAATTGCTTTTACAAGCAAAGCTTTAGGCCCACGAGCTCAAGCAATGTCCACATATGAACGGGAAATGTTGGCTATAGCACATGCAATCAAGAAGTGGCAGTCATATATTCAAGGGAGGCATTTTGTAATTAAAACTGACCACCATAGCCTGAAGTTCTTTTTACAACATCGAGCTCACACCCCTTTTCAACAGAAGTGGATTTCTAAACTTCTCGGTTTCGATTACGAGATACAATATAAACAAGGTTGTGATAATGTTGCTGCTGATGCTCTTTCTAGGATGCATTCGGATGGGGAACTGTCGGCCATCTCTTATCCTTACATGGGTTGGTTGGATGATATAAGGAGGCATAATGAACAAGATCCTTGGATAATGGACAAAATTAGGGAGTTACCTTCTGATGGTGCTTCCACATCCACAGCTCGGTACCATTTTGATAATGGTTTTCTTAAGTATAAAGGGAGGATTGTTTTAAGTCCATCTAGTGGTTGGCGGGACAAGATTTTCTATGAGCATCACTGTACTCCAGTGGCAGGTCATTCTGGTTTTCTTAAAACATATAAGCGAATTTCCCGATCTTTCTATTGGCAGGGTATGAAGTCTGATGTTATGCAGTGGGTGGCAGCTTGCTCAGTTTGTCAACAAAACAAGACTGAAACCTTGGCTTCTCCTGGCCTACTTAATCCACTTCCCATCCCATCTACTGTATGGACAGATATAGCAATGGATTTTATTGTTGGTCTTCCTCCTTGCAAAGGTCAAACCATTATTTTTGTGGTCGTTGATCGCCTTTCCAAGTATGCTCATTTCCTGCCCATGTCTCATCCATACACTGCCCATTCCGTAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 Retrotrans_gag

Protein Analysis

522

Amino Acids

59.65

Weight (kDa)

8.67

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 2 - 62 1.6e-07 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_1 PF00078 69 - 124 1.2e-09 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 187 - 281 2.5e-30 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 212 - 311 2.4e-30 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 402 - 456 6.4e-19 Integrase zinc binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019119)

Species Orthologous Gene IDs
malus_domestica MD14G1073200.v1.1
pyrus_communis pycom03g10520 pycom07g15700 pycom11g19440 pycom13g14230 pycom420g00720
rosa_roxburghii Rroxscaffold_7G00173200
rosa_wichuraiana Rw5G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1238
Acc65I GGTACC 1 cut(s) 1140
AccB1I GGYRCC 1 cut(s) 1140
AccB7I CCANNNNNTGG 1 cut(s) 1199
AciI CCGC 2 cut(s) 121, 1207
AclWI GGATC 2 cut(s) 392, 1070
AcoI YGGCCR 1 cut(s) 1017
AcsI RAATTY 4 cut(s) 25, 200, 491, 1281
AcuI CTGAAG 1 cut(s) 884
AfaI GTAC 4 cut(s) 242, 534, 1142, 1237
AfiI CCNNNNNNNGG 6 cut(s) 1037, 1115, 1137, 1199, 1247, 1469
AflII CTTAAG 1 cut(s) 1163
AgsI TTSAA 5 cut(s) 289, 556, 659, 824, 902
AjnI CCWGG 2 cut(s) 585, 1384
AluBI AGCT 9 cut(s) 20, 409, 572, 734, 749, 887, 1136, 1340, 1562
AluI AGCT 9 cut(s) 20, 409, 572, 734, 749, 887, 1136, 1340, 1562
Alw21I GWGCWC 4 cut(s) 22, 97, 751, 889
Alw26I GTCTC 3 cut(s) 282, 381, 1539
AlwI GGATC 2 cut(s) 392, 1070
AoxI GGCC 8 cut(s) 364, 428, 469, 579, 710, 739, 1017, 1387
ApeKI GCWGC 3 cut(s) 392, 974, 1337
ApoI RAATTY 4 cut(s) 25, 200, 491, 1281
Asp718I GGTACC 1 cut(s) 1140
AspS9I GGNCC 5 cut(s) 145, 364, 521, 579, 740
AsuHPI GGTGA 1 cut(s) 260
AsuII TTCGAA 1 cut(s) 107
AvaII GGWCC 2 cut(s) 145, 521
BanI GGYRCC 1 cut(s) 1140
BanII GRGCYC 4 cut(s) 22, 454, 751, 889
BauI CACGAG 2 cut(s) 236, 744
BbsI GAAGAC 2 cut(s) 173, 1448
Bbv12I GWGCWC 4 cut(s) 22, 97, 751, 889
BbvI GCAGC 3 cut(s) 404, 961, 1349
BciT130I CCWGG 2 cut(s) 587, 1386
BciVI GTATCC 1 cut(s) 60
BclI TGATCA 1 cut(s) 30
BcoDI GTCTC 3 cut(s) 282, 381, 1539
BfaI CTAG 4 cut(s) 153, 576, 990, 1197
BfmI CTRYAG 1 cut(s) 786
BfrI CTTAAG 1 cut(s) 1163
BfuAI ACCTGC 1 cut(s) 1238
BfuI GTATCC 1 cut(s) 60
BglI GCCNNNNNGGC 2 cut(s) 477, 587
BglII AGATCT 1 cut(s) 598
BisI GCNGC 3 cut(s) 393, 975, 1338
BlsI GCNGC 3 cut(s) 394, 976, 1339
Bme1390I CCNGG 2 cut(s) 587, 1386
Bme18I GGWCC 2 cut(s) 145, 521
BmgT120I GGNCC 5 cut(s) 145, 364, 521, 579, 740
BmiI GGNNCC 2 cut(s) 146, 1142
BmrFI CCNGG 2 cut(s) 587, 1386
BmsI GCATC 5 cut(s) 538, 655, 970, 984, 1237
BpiI GAAGAC 2 cut(s) 173, 1448
BpmI CTGGAG 1 cut(s) 1224
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 735
BsaBI GATNNNNATC 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 1079, 1374
Bsc4I CCNNNNNNNGG 6 cut(s) 1037, 1115, 1137, 1199, 1247, 1469
Bse1I ACTGG 2 cut(s) 481, 1241
Bse3DI GCAATG 2 cut(s) 762, 1443
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 2 cut(s) 587, 1386
BseDI CCNNGG 2 cut(s) 1079, 1374
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 6 cut(s) 1037, 1115, 1137, 1199, 1247, 1469
BseMI GCAATG 2 cut(s) 762, 1443
BseMII CTCAG 2 cut(s) 469, 1359
BseNI ACTGG 2 cut(s) 481, 1241
BseRI GAGGAG 1 cut(s) 603
BseXI GCAGC 3 cut(s) 404, 961, 1349
BshFI GGCC 8 cut(s) 366, 430, 471, 581, 712, 741, 1019, 1389
BshNI GGYRCC 1 cut(s) 1140
BsiHKAI GWGCWC 4 cut(s) 22, 97, 751, 889
BslFI GGGAC 1 cut(s) 1223
BslI CCNNNNNNNGG 6 cut(s) 1037, 1115, 1137, 1199, 1247, 1469
BsmAI GTCTC 3 cut(s) 282, 381, 1539
BsmFI GGGAC 1 cut(s) 1223
BsmI GAATGC 2 cut(s) 551, 997
BsnI GGCC 8 cut(s) 366, 430, 471, 581, 712, 741, 1019, 1389
Bsp119I TTCGAA 1 cut(s) 107
Bsp1286I GDGCHC 5 cut(s) 22, 97, 454, 751, 889
Bsp1407I TGTACA 1 cut(s) 240
Bsp143I GATC 7 cut(s) 30, 138, 397, 598, 1075, 1289, 1498
BspACI CCGC 2 cut(s) 121, 1207
BspANI GGCC 8 cut(s) 366, 430, 471, 581, 712, 741, 1019, 1389
BspCNI CTCAG 2 cut(s) 468, 1358
BspLI GGNNCC 2 cut(s) 146, 1142
BspMI ACCTGC 1 cut(s) 1238
BspPI GGATC 2 cut(s) 392, 1070
BspT104I TTCGAA 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 1140
BspTI CTTAAG 1 cut(s) 1163
BsrDI GCAATG 2 cut(s) 762, 1443
BsrGI TGTACA 1 cut(s) 240
BsrI ACTGG 2 cut(s) 481, 1241
BssECI CCNNGG 2 cut(s) 1079, 1374
BssMI GATC 7 cut(s) 30, 138, 397, 598, 1075, 1289, 1498
BssSI CACGAG 2 cut(s) 236, 744
BssT1I CCWWGG 2 cut(s) 1079, 1374
Bst2BI CACGAG 2 cut(s) 236, 744
Bst2UI CCWGG 2 cut(s) 587, 1386
Bst4CI ACNGT 5 cut(s) 245, 532, 1014, 1235, 1420
Bst6I CTCTTC 1 cut(s) 438
BstAFI CTTAAG 1 cut(s) 1163
BstAUI TGTACA 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 2 cut(s) 123, 1342
BstDEI CTNAG 4 cut(s) 327, 455, 1345, 1563
BstENI CCTNNNNNAGG 1 cut(s) 1467
BstKTI GATC 7 cut(s) 33, 141, 400, 601, 1078, 1292, 1501
BstMAI GTCTC 3 cut(s) 282, 381, 1539
BstMBI GATC 7 cut(s) 30, 138, 397, 598, 1075, 1289, 1498
BstMWI GCNNNNNNNGC 4 cut(s) 477, 578, 587, 980
BstNI CCWGG 2 cut(s) 587, 1386
BstNSI RCATGY 1 cut(s) 797
BstSCI CCNGG 2 cut(s) 585, 1384
BstSFI CTRYAG 1 cut(s) 786
BstV1I GCAGC 3 cut(s) 404, 961, 1349
BstV2I GAAGAC 2 cut(s) 173, 1448
BstX2I RGATCY 3 cut(s) 397, 598, 1075
BstYI RGATCY 3 cut(s) 397, 598, 1075
BsuI GTATCC 1 cut(s) 60
BsuRI GGCC 8 cut(s) 366, 430, 471, 581, 712, 741, 1019, 1389
BtsI GCAGTG 2 cut(s) 1334, 1545
BtsIMutI CAGTG 6 cut(s) 250, 474, 1231, 1248, 1334, 1545
BveI ACCTGC 1 cut(s) 1238
Cac8I GCNNGC 2 cut(s) 123, 1342
Cfr13I GGNCC 5 cut(s) 145, 364, 521, 579, 740
Csp6I GTAC 4 cut(s) 241, 533, 1141, 1236
CspCI CAANNNNNGTGG 2 cut(s) 890, 925
CviAII CATG 8 cut(s) 7, 185, 368, 604, 617, 794, 1036, 1531
CviQI GTAC 4 cut(s) 241, 533, 1141, 1236
DdeI CTNAG 4 cut(s) 327, 455, 1345, 1563
DpnI GATC 7 cut(s) 32, 140, 399, 600, 1077, 1291, 1500
DpnII GATC 7 cut(s) 30, 138, 397, 598, 1075, 1289, 1498
EaeI YGGCCR 1 cut(s) 1017
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 3 cut(s) 20, 749, 887
Eco130I CCWWGG 2 cut(s) 1079, 1374
Eco24I GRGCYC 4 cut(s) 22, 454, 751, 889
Eco32I GATATC 1 cut(s) 131
Eco47I GGWCC 2 cut(s) 145, 521
Eco53kI GAGCTC 3 cut(s) 20, 749, 887
Eco57I CTGAAG 1 cut(s) 884
EcoICRI GAGCTC 3 cut(s) 20, 749, 887
EcoNI CCTNNNNNAGG 1 cut(s) 1467
EcoO109I RGGNCCY 1 cut(s) 579
EcoRII CCWGG 2 cut(s) 585, 1384
EcoRV GATATC 1 cut(s) 131
EcoT14I CCWWGG 2 cut(s) 1079, 1374
EcoT22I ATGCAT 2 cut(s) 553, 999
EcoT38I GRGCYC 4 cut(s) 22, 454, 751, 889
ErhI CCWWGG 2 cut(s) 1079, 1374
FaeI CATG 8 cut(s) 10, 188, 371, 607, 620, 797, 1039, 1534
FalI AAGNNNNNCTT 4 cut(s) 320, 352, 718, 750
FaqI GGGAC 1 cut(s) 1223
FatI CATG 8 cut(s) 6, 184, 367, 603, 616, 793, 1035, 1530
FauI CCCGC 2 cut(s) 114, 1200
FauNDI CATATG 1 cut(s) 766
FbaI TGATCA 1 cut(s) 30
Fnu4HI GCNGC 3 cut(s) 393, 975, 1338
FriOI GRGCYC 4 cut(s) 22, 454, 751, 889
Fsp4HI GCNGC 3 cut(s) 393, 975, 1338
FspBI CTAG 4 cut(s) 153, 576, 990, 1197
GluI GCNGC 3 cut(s) 393, 975, 1338
GsuI CTGGAG 1 cut(s) 1224
HaeIII GGCC 8 cut(s) 366, 430, 471, 581, 712, 741, 1019, 1389
Hin1II CATG 8 cut(s) 10, 188, 371, 607, 620, 797, 1039, 1534
HincII GTYRAC 1 cut(s) 1355
HindII GTYRAC 1 cut(s) 1355
HindIII AAGCTT 2 cut(s) 407, 732
HinfI GANTC 2 cut(s) 248, 257
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 5 cut(s) 241, 521, 561, 762, 1355
Hpy188I TCNGA 5 cut(s) 138, 501, 1003, 1114, 1318
Hpy188III TCNNGA 2 cut(s) 802, 1287
Hpy8I GTNNAC 5 cut(s) 241, 521, 561, 762, 1355
HpyAV CCTTC 4 cut(s) 592, 658, 698, 1119
HpyCH4III ACNGT 5 cut(s) 245, 532, 1014, 1235, 1420
HpyCH4V TGCA 6 cut(s) 6, 551, 797, 997, 1327, 1467
HpyF10VI GCNNNNNNNGC 4 cut(s) 477, 578, 587, 980
HpyF3I CTNAG 4 cut(s) 327, 455, 1345, 1563
Hsp92II CATG 8 cut(s) 10, 188, 371, 607, 620, 797, 1039, 1534
KpnI GGTACC 1 cut(s) 1144
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 7 cut(s) 30, 138, 397, 598, 1075, 1289, 1498
Lsp1109I GCAGC 3 cut(s) 404, 961, 1349
LweI GCATC 5 cut(s) 538, 655, 970, 984, 1237
MaeI CTAG 4 cut(s) 153, 576, 990, 1197
MaeIII GTNAC 5 cut(s) 155, 191, 245, 332, 1104
MalI GATC 7 cut(s) 32, 140, 399, 600, 1077, 1291, 1500
MboI GATC 7 cut(s) 30, 138, 397, 598, 1075, 1289, 1498
MboII GAAGA 4 cut(s) 178, 257, 425, 1448
MfeI CAATTG 2 cut(s) 308, 714
MflI RGATCY 3 cut(s) 397, 598, 1075
MhlI GDGCHC 5 cut(s) 22, 97, 454, 751, 889
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 3 cut(s) 57, 93, 1026
MnlI CCTC 9 cut(s) 441, 447, 482, 624, 702, 822, 1053, 1170, 1470
Mph1103I ATGCAT 2 cut(s) 553, 999
MseI TTAA 7 cut(s) 62, 411, 843, 1164, 1187, 1266, 1395
MslI CAYNNNNRTG 3 cut(s) 153, 556, 1002
MspCI CTTAAG 1 cut(s) 1163
MspR9I CCNGG 2 cut(s) 587, 1386
MunI CAATTG 2 cut(s) 308, 714
Mva1269I GAATGC 2 cut(s) 551, 997
MvaI CCWGG 2 cut(s) 587, 1386
MwoI GCNNNNNNNGC 4 cut(s) 477, 578, 587, 980
NdeI CATATG 1 cut(s) 766
NdeII GATC 7 cut(s) 30, 138, 397, 598, 1075, 1289, 1498
NlaIII CATG 8 cut(s) 10, 188, 371, 607, 620, 797, 1039, 1534
NlaIV GGNNCC 2 cut(s) 146, 1142
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 4 cut(s) 155, 191, 245, 332
NsiI ATGCAT 2 cut(s) 553, 999
NspI RCATGY 1 cut(s) 797
NspV TTCGAA 1 cut(s) 107
PctI GAATGC 2 cut(s) 551, 997
PfeI GAWTC 1 cut(s) 257
PflMI CCANNNNNTGG 1 cut(s) 1199
PkrI GCNGC 3 cut(s) 394, 976, 1339
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
Psp124BI GAGCTC 3 cut(s) 22, 751, 889
Psp6I CCWGG 2 cut(s) 585, 1384
PspGI CCWGG 2 cut(s) 585, 1384
PspN4I GGNNCC 2 cut(s) 146, 1142
PspPI GGNCC 5 cut(s) 145, 364, 521, 579, 740
PsuI RGATCY 3 cut(s) 397, 598, 1075
RsaI GTAC 4 cut(s) 242, 534, 1142, 1237
RsaNI GTAC 4 cut(s) 241, 533, 1141, 1236
RseI CAYNNNNRTG 3 cut(s) 153, 556, 1002
SacI GAGCTC 3 cut(s) 22, 751, 889
SaqAI TTAA 7 cut(s) 62, 411, 843, 1164, 1187, 1266, 1395
SatI GCNGC 3 cut(s) 393, 975, 1338
Sau3AI GATC 7 cut(s) 30, 138, 397, 598, 1075, 1289, 1498
Sau96I GGNCC 5 cut(s) 145, 364, 521, 579, 740
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 2 cut(s) 587, 1386
SduI GDGCHC 5 cut(s) 22, 97, 454, 751, 889
SfaNI GCATC 5 cut(s) 538, 655, 970, 984, 1237
SfcI CTRYAG 1 cut(s) 786
SfuI TTCGAA 1 cut(s) 107
SinI GGWCC 2 cut(s) 145, 521
SmiMI CAYNNNNRTG 3 cut(s) 153, 556, 1002
SmlI CTYRAG 2 cut(s) 750, 1163
SmoI CTYRAG 2 cut(s) 750, 1163
SsiI CCGC 2 cut(s) 121, 1207
SspI AATATT 1 cut(s) 359
SspMI CTAG 4 cut(s) 153, 576, 990, 1197
SstI GAGCTC 3 cut(s) 22, 751, 889
StyD4I CCNGG 2 cut(s) 585, 1384
StyI CCWWGG 2 cut(s) 1079, 1374
TaaI ACNGT 5 cut(s) 245, 532, 1014, 1235, 1420
TaqI TCGA 5 cut(s) 107, 354, 487, 883, 933
TatI WGTACW 3 cut(s) 240, 532, 1235
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 7 cut(s) 62, 411, 843, 1164, 1187, 1266, 1395
Tru9I TTAA 7 cut(s) 62, 411, 843, 1164, 1187, 1266, 1395
TscAI CASTG 6 cut(s) 250, 481, 1238, 1248, 1334, 1552
TseFI GTSAC 4 cut(s) 155, 191, 245, 332
TseI GCWGC 3 cut(s) 392, 974, 1337
Tsp45I GTSAC 4 cut(s) 155, 191, 245, 332
TspDTI ATGAA 8 cut(s) 84, 213, 484, 582, 640, 783, 1083, 1325
TspGWI ACGGA 2 cut(s) 522, 1546
TspRI CASTG 6 cut(s) 250, 481, 1238, 1248, 1334, 1552
Van91I CCANNNNNTGG 1 cut(s) 1199
Vha464I CTTAAG 1 cut(s) 1163
VpaK11BI GGWCC 2 cut(s) 145, 521
XagI CCTNNNNNAGG 1 cut(s) 1467
XapI RAATTY 4 cut(s) 25, 200, 491, 1281
XceI RCATGY 1 cut(s) 797
XcmI CCANNNNNNNNNTGG 4 cut(s) 1151, 1200, 1419, 1486
XspI CTAG 4 cut(s) 153, 576, 990, 1197
Zsp2I ATGCAT 2 cut(s) 553, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.