Rw5G041380

Retroviral aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0019119
Physical Location & Seq
Reverse (-)
72931785 .. 72934616
2832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G041380.1

Sequence Viewer

Length: 2832 bp
ATGGATCCTCGTGTGGAGAAGCTAGAGGCTGATGTTGGCTTGCTCACCACTAGTGTTAGCGCGCTTGCCATTGACCAGAAGAAAATCATGGATCAATTGGCTGAGGTGGTTTCACAGCTCAAGTCCAAATCCAACTTTGAAGAGGGAGAGAAATCTACCAATTTCAAGCAAATTTTCGGTGACCGATCTGATGGAAGTCATAAAGGTGGACCAACTTACAATCCTAAGACAGTCAAGCTTGACTTTCCTCGTTTCAGGGGCGATGAGGACACCACAAGCTGGGTTTGCAGGGCTGAGCAATTTTTCCGTTTTCATCACACTCCCGACGAAGAACGGGTGGAACTAGCATCATTCCATTTGGAGGGAGACGCTCAATTATGGTTCCAACTTTTGATTCAAGATGGTGGGGAGGTGTCCTGGCGAAAGTTTTGTGATGGGCTACATGCAAGATTCGGCCCTAATCAGTTTCAAGATTTCTTCGCTGAGTTAGCAAAACTAGAGCAACATGGTTCTGTTCGTGAGTTTCAATCTCGCTTTGAAAGGCTATTGCCTAAAGTTGGGCCTCTTTCACAAGAGAGGCAGGTGAGTTTTTTCATTAGTGGGTTGAAAACAATCCTCAAAGCTGATGTTTTGGCTGGTCGTCCTACTTCCCTCTCGGATGCTATTGGTCTTGCACTACTTTTTGAAGCTCGATATGAATCCCAGCGGCGTATTGTGCCAGCAATCATGGAGCAAAAAAATACCCAAATTTCTGCTCATACAGAAACAACAAATCGCACTCCACTTGTTCGACGAATGTCCCCTGTCGAGTTGCAAGAGAGACGAGCAAAAGGATTGTGCTACAACTGTAATGAGAGGTTTGTTCCTGGACATAGATGCAAAAAAATTTTCTTAATTGATGCTATTACTGAAGAAGAAGATGGAGATGTTATGATGGACGAAGGAGAAGCTGTGTTTGAAGACGCCAATGTTCTGCCAGAAATTTCTCTACATGCTATTTCAGGCTCTAGTGCAGCAAATACTATGCGGGTGAATGGAAGCATTGAGCATACTACAACTACTATTCTGGTAGATTCGGGCAGTACTCATAATTTCATGAATGAGAACCTTGCTAAACAAATGGGACTACAACCTGAAAACTCAAGAAAGTTTCAAGTTATGGTAGCTTCAGGTGAAAGATTGTCCAGTCAAGGTAAGTGTACAAATGTCCAGTTGAATTTGCAAGGATTGACTATTTTTGTTGACCTCTACTTGCTTCCATTAGAGGGCTATGATGTAGTTTTGGGGACCCAATGGCTGCGTACACTTGGCCCAATTTTGTGGGACTTTGAAAAATTACAGATGAAGTTTAAGTTGGCTGGCAAGGTGGTGAGTCTTCAAGGCCTCTCAACTCCCAGAAATAAGTGGGTTGGAGACTTGCGATTCAACAGAGAGATTAAGAAAAGCATAAGAGGAGTATTTTTGAGGTTGAATTGTATGGAGAAGCAGACAACAAAAAAGCATGAGCTCTCACCTCACCAATTGCAACAAATTTTGGGTGAGTATAAAGATATATTCCAAGAGCCAAGAGGACTGCCTCCCTCAAGAGTCCATGATCACCGAATTCCACTGAAACAAGGAGTTGATCCAGTTTGTGTCAAGCCATATCGATACCCGCAGTATCAGAAATCGGAGATAGAGAAAATAGTGAAGGAGATGCTTACTACAGGAGTCATCAAGTCAAGTAACAGCCCTTACTCATCCCCTGTCATTTTAGTTAAAAAACATGATGGATCTTGGCGTATGTGTGTTGATTACAGGGCCCTCAATCAAATCACTATTAAGGATAAATTTCCTATTCCAGTCATCGATGAGTTACTTGATGAGTTACATGGGTCAAGAGTCTTTACAAAGTTGGATTTGCGCAGTGGGTATCATCAGATTTTGGTAGTGCTGGATGACAGACCAAAAACAGCATTTCGAACACATCATGGGCATTACGAATTTTCAGTCATGCCGTTTGGTCTCACAAATGCACCATCCACCTTCCAAGCTTTGATGAATGAGGTATTTCAAAGTTATCTGCGGAAACTCATCTTAGTATTTTTTGATGATATTCTTGTTTATAGCAAAACATGGGATAAACATGTTCAGCACTTGCAGATTACATTCTCTGTATTAAAAACTCACAAATTGTTTGTAAAAAAAGAGAAATGTCAGTTTGGCCAAAGTGAGGTACAATACCTTGGCCATGTGATCTCAGGTTCTGAAGTTGCTGTGGATCCTGACAAGATTGTTGTGATGACTAATTGGCCTAAACCAAATTCCTTGAAGGCACTTAGAGGATTTTTGGGGTTAACAGGCAATTACAGGAAGTTCATTCAGCATTTTGGCAAAATTGCTGCACCACTCACACATATGTTGAAGAAAGATTCATTCTTGTGGACACCTGCAGCTGAAAAATCATTCCTACAATTGAAGGAAGTGATGACTAAGGCACGGGTATTGGCTTTACCCGATTTCTCAAAAACTTTTATAGTGGAATGTGATGCTAGTGGGTCAGGTATTGGAGCTGTATTAATGCAAGAGAGACCTATTGCATTTTTTAGTCAAGCACTGCATGGAAAGAACTTGTTGTTGTCCACTTATGAGAAGGAGATCCTCGCCTTGGTGTTATCGGTGCAAAGATGGCGTCCATACTTATTGGGGAGTCAATTTCAGGTGCGAACTGACCAACACAGTTTGAAGTATTTGTGGAACCAAAAGATTACAACCAAGCTCAGCAGAGGTGGCTTTACAAGCTTATGGGTTTTGACTTCATTATTGAGTACAAAAGGGGGAAGGAAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2

Protein Analysis

943

Amino Acids

107.42

Weight (kDa)

9.16

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 102 - 252 1.9e-14 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 115 - 203 9.5e-14 Retrotransposon gag protein
Asp_protease_2 PF13650 343 - 431 1e-07 Aspartyl protease
gag-asp_proteas PF13975 344 - 434 1.9e-10 gag-polyprotein putative aspartyl protease
RVP_2 PF08284 347 - 434 5.9e-12 Retroviral aspartyl protease
RVT_1 PF00078 586 - 746 2.9e-41 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 808 - 901 5.8e-28 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 833 - 920 1.7e-25 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019119)

Species Orthologous Gene IDs
malus_domestica MD14G1073200.v1.1
pyrus_communis pycom03g10520 pycom07g15700 pycom11g19440 pycom13g14230 pycom420g00720
rosa_roxburghii Rroxscaffold_7G00173200
rosa_wichuraiana Rw5G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 571, 2437
Acc16I TGCGCA 1 cut(s) 1904
Acc36I ACCTGC 2 cut(s) 571, 2437
AccB7I CCANNNNNTGG 1 cut(s) 279
AccII CGCG 1 cut(s) 62
AciI CCGC 4 cut(s) 706, 1027, 1655, 2065
AclWI GGATC 7 cut(s) 12, 99, 1619, 1780, 2255, 2268, 2632
AcoI YGGCCR 2 cut(s) 2203, 2227
AcuI CTGAAG 3 cut(s) 930, 1152, 2268
AcyI GRCGYC 2 cut(s) 963, 2671
AfaI GTAC 5 cut(s) 1084, 1201, 1303, 2217, 2809
AfiI CCNNNNNNNGG 6 cut(s) 279, 361, 557, 1265, 2212, 2647
AflIII ACRYGT 1 cut(s) 2125
AhlI ACTAGT 1 cut(s) 50
AjnI CCWGG 2 cut(s) 416, 865
AjuI GAANNNNNNNTTGG 4 cut(s) 378, 410, 1337, 1369
Alw21I GWGCWC 1 cut(s) 1509
Alw26I GTCTC 5 cut(s) 360, 814, 1407, 2009, 2563
AlwI GGATC 7 cut(s) 12, 99, 1619, 1780, 2255, 2268, 2632
AlwNI CAGNNNCTG 1 cut(s) 2246
AoxI GGCC 8 cut(s) 454, 560, 1309, 1381, 1800, 2203, 2227, 2291
ApaI GGGCCC 1 cut(s) 1804
ApeKI GCWGC 4 cut(s) 1013, 1297, 2381, 2432
ArsI GACNNNNNNTTYG 4 cut(s) 1974, 2006, 2656, 2688
AseI ATTAAT 1 cut(s) 2558
AspLEI GCGC 3 cut(s) 62, 64, 1905
AspS9I GGNCC 7 cut(s) 209, 455, 560, 1287, 1310, 1800, 1801
AsuII TTCGAA 1 cut(s) 1960
AvaII GGWCC 2 cut(s) 209, 1287
BaeGI GKGCMC 1 cut(s) 1804
BalI TGGCCA 2 cut(s) 2205, 2229
BamHI GGATCC 2 cut(s) 4, 2260
BanII GRGCYC 2 cut(s) 1509, 1804
BauI CACGAG 1 cut(s) 9
BbsI GAAGAC 2 cut(s) 966, 1367
Bbv12I GWGCWC 1 cut(s) 1509
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 4 cut(s) 1025, 1284, 2368, 2444
BccI CCATC 8 cut(s) 185, 395, 428, 914, 928, 1763, 2026, 2661
BceAI ACGGC 1 cut(s) 1981
BciT130I CCWGG 2 cut(s) 418, 867
BclI TGATCA 1 cut(s) 1594
BcoDI GTCTC 5 cut(s) 360, 814, 1407, 2009, 2563
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 6 cut(s) 23, 51, 344, 497, 1008, 2532
BfmI CTRYAG 2 cut(s) 1704, 2430
BfuAI ACCTGC 2 cut(s) 571, 2437
BisI GCNGC 5 cut(s) 707, 1014, 1298, 2382, 2433
BlpI GCTNAGC 2 cut(s) 294, 2759
BlsI GCNGC 5 cut(s) 708, 1015, 1299, 2383, 2434
BmcAI AGTACT 1 cut(s) 1084
Bme1390I CCNGG 2 cut(s) 418, 867
Bme18I GGWCC 2 cut(s) 209, 1287
BmgT120I GGNCC 7 cut(s) 209, 455, 560, 1287, 1310, 1800, 1801
BmiI GGNNCC 7 cut(s) 6, 383, 1288, 1289, 1802, 2262, 2738
BmrFI CCNGG 2 cut(s) 418, 867
BmsI GCATC 6 cut(s) 356, 649, 866, 889, 1686, 2518
BoxI GACNNNNGTC 1 cut(s) 796
BpiI GAAGAC 2 cut(s) 966, 1367
Bpu10I CCTNAGC 1 cut(s) 102
Bpu1102I GCTNAGC 2 cut(s) 294, 2759
Bpu14I TTCGAA 1 cut(s) 1960
BpuEI CTTGAG 3 cut(s) 104, 1126, 1567
Bsa29I ATCGAT 2 cut(s) 1648, 1848
BsaBI GATNNNNATC 1 cut(s) 697
BsaHI GRCGYC 2 cut(s) 963, 2671
BsaI GGTCTC 2 cut(s) 2009, 2563
BsaJI CCNNGG 2 cut(s) 2224, 2646
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 6 cut(s) 279, 361, 557, 1265, 2212, 2647
Bse1I ACTGG 4 cut(s) 1185, 1210, 1628, 1841
Bse8I GATNNNNATC 1 cut(s) 697
BseBI CCWGG 2 cut(s) 418, 867
BseCI ATCGAT 2 cut(s) 1648, 1848
BseDI CCNNGG 2 cut(s) 2224, 2646
BseGI GGATG 4 cut(s) 664, 1739, 1942, 2018
BseJI GATNNNNATC 1 cut(s) 697
BseLI CCNNNNNNNGG 6 cut(s) 279, 361, 557, 1265, 2212, 2647
BseMII CTCAG 5 cut(s) 93, 285, 474, 2253, 2773
BseNI ACTGG 4 cut(s) 1185, 1210, 1628, 1841
BsePI GCGCGC 1 cut(s) 60
BseRI GAGGAG 1 cut(s) 1467
BseSI GKGCMC 1 cut(s) 1804
BseXI GCAGC 4 cut(s) 1025, 1284, 2368, 2444
BseYI CCCAGC 2 cut(s) 279, 702
BsgI GTGCAG 2 cut(s) 1032, 2367
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 8 cut(s) 456, 562, 1311, 1383, 1802, 2205, 2229, 2293
BshVI ATCGAT 2 cut(s) 1648, 1848
BsiHKAI GWGCWC 1 cut(s) 1509
BslFI GGGAC 4 cut(s) 784, 1137, 1300, 1337
BslI CCNNNNNNNGG 6 cut(s) 279, 361, 557, 1265, 2212, 2647
BsmAI GTCTC 5 cut(s) 360, 814, 1407, 2009, 2563
BsmBI CGTCTC 2 cut(s) 360, 814
BsmFI GGGAC 4 cut(s) 784, 1137, 1300, 1337
BsnI GGCC 8 cut(s) 456, 562, 1311, 1383, 1802, 2205, 2229, 2293
Bso31I GGTCTC 2 cut(s) 2009, 2563
Bsp119I TTCGAA 1 cut(s) 1960
Bsp120I GGGCCC 1 cut(s) 1800
Bsp1286I GDGCHC 2 cut(s) 1509, 1804
Bsp1407I TGTACA 1 cut(s) 1199
Bsp143I GATC 9 cut(s) 4, 91, 185, 1594, 1624, 1772, 2235, 2260, 2637
Bsp1720I GCTNAGC 2 cut(s) 294, 2759
BspACI CCGC 4 cut(s) 706, 1027, 1655, 2065
BspANI GGCC 8 cut(s) 456, 562, 1311, 1383, 1802, 2205, 2229, 2293
BspCNI CTCAG 5 cut(s) 94, 286, 475, 2252, 2772
BspDI ATCGAT 2 cut(s) 1648, 1848
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 1095
BspLI GGNNCC 7 cut(s) 6, 383, 1288, 1289, 1802, 2262, 2738
BspMAI CTGCAG 1 cut(s) 2434
BspMI ACCTGC 2 cut(s) 571, 2437
BspPI GGATC 7 cut(s) 12, 99, 1619, 1780, 2255, 2268, 2632
BspT104I TTCGAA 1 cut(s) 1960
BspTNI GGTCTC 2 cut(s) 2009, 2563
BsrGI TGTACA 1 cut(s) 1199
BsrI ACTGG 4 cut(s) 1185, 1210, 1628, 1841
BssECI CCNNGG 2 cut(s) 2224, 2646
BssHII GCGCGC 1 cut(s) 60
BssMI GATC 9 cut(s) 4, 91, 185, 1594, 1624, 1772, 2235, 2260, 2637
BssNI GRCGYC 2 cut(s) 963, 2671
BssSI CACGAG 1 cut(s) 9
BssT1I CCWWGG 2 cut(s) 2224, 2646
Bst2BI CACGAG 1 cut(s) 9
Bst2UI CCWGG 2 cut(s) 418, 867
Bst4CI ACNGT 3 cut(s) 232, 848, 2720
Bst6I CTCTTC 1 cut(s) 135
BstACI GRCGYC 2 cut(s) 963, 2671
BstAUI TGTACA 1 cut(s) 1199
BstBI TTCGAA 1 cut(s) 1960
BstC8I GCNNGC 5 cut(s) 41, 62, 66, 720, 1360
BstDEI CTNAG 9 cut(s) 102, 225, 294, 483, 2077, 2239, 2318, 2472, 2759
BstEII GGTNACC 1 cut(s) 179
BstF5I GGATG 4 cut(s) 664, 1739, 1942, 2018
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 3 cut(s) 62, 64, 1905
BstKTI GATC 9 cut(s) 7, 94, 188, 1597, 1627, 1775, 2238, 2263, 2640
BstMAI GTCTC 5 cut(s) 360, 814, 1407, 2009, 2563
BstMBI GATC 9 cut(s) 4, 91, 185, 1594, 1624, 1772, 2235, 2260, 2637
BstMWI GCNNNNNNNGC 6 cut(s) 285, 488, 715, 2668, 2769, 2778
BstNI CCWGG 2 cut(s) 418, 867
BstNSI RCATGY 3 cut(s) 446, 995, 2129
BstPAI GACNNNNGTC 1 cut(s) 796
BstPI GGTNACC 1 cut(s) 179
BstSCI CCNGG 2 cut(s) 416, 865
BstSFI CTRYAG 2 cut(s) 1704, 2430
BstSLI GKGCMC 1 cut(s) 1804
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 4 cut(s) 1025, 1284, 2368, 2444
BstV2I GAAGAC 2 cut(s) 966, 1367
BstX2I RGATCY 4 cut(s) 4, 1772, 2260, 2637
BstXI CCANNNNNNTGG 1 cut(s) 1320
BstYI RGATCY 4 cut(s) 4, 1772, 2260, 2637
Bsu15I ATCGAT 2 cut(s) 1648, 1848
BsuRI GGCC 8 cut(s) 456, 562, 1311, 1383, 1802, 2205, 2229, 2293
BsuTUI ATCGAT 2 cut(s) 1648, 1848
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 4 cut(s) 664, 1739, 1942, 2018
BtsI GCAGTG 2 cut(s) 1912, 2594
BtsIMutI CAGTG 3 cut(s) 1607, 1912, 2594
BveI ACCTGC 2 cut(s) 571, 2437
Cac8I GCNNGC 5 cut(s) 41, 62, 66, 720, 1360
CaiI CAGNNNCTG 1 cut(s) 2246
CciI TCATGA 1 cut(s) 1095
CfoI GCGC 3 cut(s) 62, 64, 1905
Cfr13I GGNCC 7 cut(s) 209, 455, 560, 1287, 1310, 1800, 1801
ClaI ATCGAT 2 cut(s) 1648, 1848
CseI GACGC 3 cut(s) 377, 971, 2660
Csp6I GTAC 5 cut(s) 1083, 1200, 1302, 2216, 2808
CviQI GTAC 5 cut(s) 1083, 1200, 1302, 2216, 2808
DdeI CTNAG 9 cut(s) 102, 225, 294, 483, 2077, 2239, 2318, 2472, 2759
DpnI GATC 9 cut(s) 6, 93, 187, 1596, 1626, 1774, 2237, 2262, 2639
DpnII GATC 9 cut(s) 4, 91, 185, 1594, 1624, 1772, 2235, 2260, 2637
EaeI YGGCCR 2 cut(s) 2203, 2227
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 1507
Eco130I CCWWGG 2 cut(s) 2224, 2646
Eco147I AGGCCT 1 cut(s) 1383
Eco24I GRGCYC 2 cut(s) 1509, 1804
Eco31I GGTCTC 2 cut(s) 2009, 2563
Eco47I GGWCC 2 cut(s) 209, 1287
Eco53kI GAGCTC 1 cut(s) 1507
Eco57I CTGAAG 3 cut(s) 930, 1152, 2268
Eco91I GGTNACC 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 1507
EcoO109I RGGNCCY 3 cut(s) 1287, 1800, 1801
EcoO65I GGTNACC 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 1602
EcoRII CCWGG 2 cut(s) 416, 865
EcoT14I CCWWGG 2 cut(s) 2224, 2646
EcoT38I GRGCYC 2 cut(s) 1509, 1804
ErhI CCWWGG 2 cut(s) 2224, 2646
Esp3I CGTCTC 2 cut(s) 360, 814
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 4 cut(s) 784, 1137, 1300, 1337
FauI CCCGC 2 cut(s) 1020, 1662
FauNDI CATATG 1 cut(s) 2397
FbaI TGATCA 1 cut(s) 1594
Fnu4HI GCNGC 5 cut(s) 707, 1014, 1298, 2382, 2433
FokI GGATG 4 cut(s) 671, 1726, 1949, 2005
FriOI GRGCYC 2 cut(s) 1509, 1804
Fsp4HI GCNGC 5 cut(s) 707, 1014, 1298, 2382, 2433
FspBI CTAG 6 cut(s) 23, 51, 344, 497, 1008, 2532
FspI TGCGCA 1 cut(s) 1904
GlaI GCGC 3 cut(s) 61, 63, 1904
GluI GCNGC 5 cut(s) 707, 1014, 1298, 2382, 2433
GsaI CCCAGC 2 cut(s) 283, 706
HaeIII GGCC 8 cut(s) 456, 562, 1311, 1383, 1802, 2205, 2229, 2293
HgaI GACGC 3 cut(s) 377, 971, 2660
HhaI GCGC 3 cut(s) 62, 64, 1905
Hin1I GRCGYC 2 cut(s) 963, 2671
Hin6I GCGC 3 cut(s) 60, 62, 1903
HinP1I GCGC 3 cut(s) 60, 62, 1903
HincII GTYRAC 2 cut(s) 1243, 2337
HindII GTYRAC 2 cut(s) 1243, 2337
HindIII AAGCTT 3 cut(s) 236, 2031, 2779
HpaI GTTAAC 1 cut(s) 2337
Hpy166II GTNNAC 7 cut(s) 209, 1200, 1243, 1304, 2337, 2424, 2622
Hpy188I TCNGA 6 cut(s) 190, 658, 1665, 1672, 1920, 2248
Hpy188III TCNNGA 9 cut(s) 323, 398, 470, 518, 1096, 1143, 1584, 1878, 2264
Hpy8I GTNNAC 7 cut(s) 209, 1200, 1243, 1304, 2337, 2424, 2622
Hpy99I CGWCG 2 cut(s) 329, 795
HpyAV CCTTC 7 cut(s) 935, 1684, 2035, 2305, 2452, 2626, 2814
HpyCH4III ACNGT 3 cut(s) 232, 848, 2720
HpyF10VI GCNNNNNNNGC 6 cut(s) 285, 488, 715, 2668, 2769, 2778
HpyF3I CTNAG 9 cut(s) 102, 225, 294, 483, 2077, 2239, 2318, 2472, 2759
Hsp92I GRCGYC 2 cut(s) 963, 2671
HspAI GCGC 3 cut(s) 60, 62, 1903
KflI GGGWCCC 1 cut(s) 1287
Ksp22I TGATCA 1 cut(s) 1594
KspAI GTTAAC 1 cut(s) 2337
Kzo9I GATC 9 cut(s) 4, 91, 185, 1594, 1624, 1772, 2235, 2260, 2637
LmnI GCTCC 2 cut(s) 730, 2549
Lsp1109I GCAGC 4 cut(s) 1025, 1284, 2368, 2444
LweI GCATC 6 cut(s) 356, 649, 866, 889, 1686, 2518
MaeI CTAG 6 cut(s) 23, 51, 344, 497, 1008, 2532
MaeIII GTNAC 4 cut(s) 179, 1724, 1854, 1866
MalI GATC 9 cut(s) 6, 93, 187, 1596, 1626, 1774, 2237, 2262, 2639
MboI GATC 9 cut(s) 4, 91, 185, 1594, 1624, 1772, 2235, 2260, 2637
MfeI CAATTG 3 cut(s) 95, 1520, 2453
MflI RGATCY 4 cut(s) 4, 1772, 2260, 2637
MhlI GDGCHC 2 cut(s) 1509, 1804
MlsI TGGCCA 2 cut(s) 2205, 2229
MluNI TGGCCA 2 cut(s) 2205, 2229
MlyI GAGTC 5 cut(s) 1381, 1596, 1719, 1890, 2698
MmeI TCCRAC 4 cut(s) 156, 409, 1390, 1875
Mox20I TGGCCA 2 cut(s) 2205, 2229
MscI TGGCCA 2 cut(s) 2205, 2229
MseI TTAA 8 cut(s) 893, 1350, 1437, 1758, 1821, 2159, 2336, 2558
MslI CAYNNNNRTG 3 cut(s) 204, 2396, 2419
Msp20I TGGCCA 2 cut(s) 2205, 2229
MspA1I CMGCKG 2 cut(s) 706, 2435
MspR9I CCNGG 2 cut(s) 418, 867
MunI CAATTG 3 cut(s) 95, 1520, 2453
MvaI CCWGG 2 cut(s) 418, 867
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 6 cut(s) 285, 488, 715, 2668, 2769, 2778
NdeI CATATG 1 cut(s) 2397
NdeII GATC 9 cut(s) 4, 91, 185, 1594, 1624, 1772, 2235, 2260, 2637
NlaIV GGNNCC 7 cut(s) 6, 383, 1288, 1289, 1802, 2262, 2738
NmuCI GTSAC 1 cut(s) 179
NsbI TGCGCA 1 cut(s) 1904
NspI RCATGY 3 cut(s) 446, 995, 2129
NspV TTCGAA 1 cut(s) 1960
PagI TCATGA 1 cut(s) 1095
PaqCI CACCTGC 2 cut(s) 571, 2437
PauI GCGCGC 1 cut(s) 60
PceI AGGCCT 1 cut(s) 1383
PciI ACATGT 1 cut(s) 2125
PfeI GAWTC 6 cut(s) 394, 450, 698, 1073, 1422, 2411
PflMI CCANNNNNTGG 1 cut(s) 279
PfoI TCCNGGA 1 cut(s) 865
PkrI GCNGC 5 cut(s) 708, 1015, 1299, 2383, 2434
PleI GAGTC 5 cut(s) 1380, 1595, 1718, 1889, 2697
PpsI GAGTC 5 cut(s) 1380, 1595, 1718, 1889, 2697
PpuMI RGGWCCY 1 cut(s) 1287
PscI ACATGT 1 cut(s) 2125
PshAI GACNNNNGTC 1 cut(s) 796
PshBI ATTAAT 1 cut(s) 2558
Psp124BI GAGCTC 1 cut(s) 1509
Psp5II RGGWCCY 1 cut(s) 1287
Psp6I CCWGG 2 cut(s) 416, 865
PspEI GGTNACC 1 cut(s) 179
PspFI CCCAGC 2 cut(s) 279, 702
PspGI CCWGG 2 cut(s) 416, 865
PspN4I GGNNCC 7 cut(s) 6, 383, 1288, 1289, 1802, 2262, 2738
PspOMI GGGCCC 1 cut(s) 1800
PspPI GGNCC 7 cut(s) 209, 455, 560, 1287, 1310, 1800, 1801
PspPPI RGGWCCY 1 cut(s) 1287
PstI CTGCAG 1 cut(s) 2434
PstNI CAGNNNCTG 1 cut(s) 2246
PsuI RGATCY 4 cut(s) 4, 1772, 2260, 2637
PteI GCGCGC 1 cut(s) 60
PvuII CAGCTG 1 cut(s) 2435
RsaI GTAC 5 cut(s) 1084, 1201, 1303, 2217, 2809
RsaNI GTAC 5 cut(s) 1083, 1200, 1302, 2216, 2808
RseI CAYNNNNRTG 3 cut(s) 204, 2396, 2419
SacI GAGCTC 1 cut(s) 1509
SaqAI TTAA 8 cut(s) 893, 1350, 1437, 1758, 1821, 2159, 2336, 2558
SatI GCNGC 5 cut(s) 707, 1014, 1298, 2382, 2433
Sau3AI GATC 9 cut(s) 4, 91, 185, 1594, 1624, 1772, 2235, 2260, 2637
Sau96I GGNCC 7 cut(s) 209, 455, 560, 1287, 1310, 1800, 1801
ScaI AGTACT 1 cut(s) 1084
SchI GAGTC 5 cut(s) 1381, 1596, 1719, 1890, 2698
ScrFI CCNGG 2 cut(s) 418, 867
SduI GDGCHC 2 cut(s) 1509, 1804
SfaNI GCATC 6 cut(s) 356, 649, 866, 889, 1686, 2518
SfcI CTRYAG 2 cut(s) 1704, 2430
SfuI TTCGAA 1 cut(s) 1960
SinI GGWCC 2 cut(s) 209, 1287
SmiMI CAYNNNNRTG 3 cut(s) 204, 2396, 2419
SmlI CTYRAG 3 cut(s) 119, 1141, 1582
SmoI CTYRAG 3 cut(s) 119, 1141, 1582
SpeI ACTAGT 1 cut(s) 50
SseBI AGGCCT 1 cut(s) 1383
SsiI CCGC 4 cut(s) 706, 1027, 1655, 2065
SspMI CTAG 6 cut(s) 23, 51, 344, 497, 1008, 2532
SstI GAGCTC 1 cut(s) 1509
StuI AGGCCT 1 cut(s) 1383
StyD4I CCNGG 2 cut(s) 416, 865
StyI CCWWGG 2 cut(s) 2224, 2646
TaaI ACNGT 3 cut(s) 232, 848, 2720
TaqI TCGA 6 cut(s) 691, 790, 807, 1648, 1848, 1960
TaqII GACCGA 1 cut(s) 198
TatI WGTACW 3 cut(s) 1082, 1199, 2807
TauI GCSGC 1 cut(s) 709
TfiI GAWTC 6 cut(s) 394, 450, 698, 1073, 1422, 2411
Tru1I TTAA 8 cut(s) 893, 1350, 1437, 1758, 1821, 2159, 2336, 2558
Tru9I TTAA 8 cut(s) 893, 1350, 1437, 1758, 1821, 2159, 2336, 2558
TscAI CASTG 3 cut(s) 1614, 1912, 2601
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 4 cut(s) 1013, 1297, 2381, 2432
Tsp45I GTSAC 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 3 cut(s) 1614, 1912, 2601
Van91I CCANNNNNTGG 1 cut(s) 279
VpaK11BI GGWCC 2 cut(s) 209, 1287
VspI ATTAAT 1 cut(s) 2558
XceI RCATGY 3 cut(s) 446, 995, 2129
XspI CTAG 6 cut(s) 23, 51, 344, 497, 1008, 2532
ZrmI AGTACT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.